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A paper radioimmunosorbent test (PRIST) for the detection of larva-specific antibodies to toxocara canis in human sera
- Source :
- Journal of Immunological Methods. 37:47-55
- Publication Year :
- 1980
- Publisher :
- Elsevier BV, 1980.
-
Abstract
- A paper radioimmunosorbent test (PRIST) was shown to be sensitive and reproducible when used with excretory/secretory antigen of Toxocara canis second stage larvae. Whatman No. 50 filter paper (5 mm discs) gave the most consistent and clear results with antigen at a concentration of 100 micrograms/ml, and could be stored for up to 3 weeks in vacuo at -70 degrees C. Antigen coated discs were incubated with test sera at 1:10 dilution for 3 h at room temperature (21 degrees C), reacted with [125I]anti-human IgG for 1 h and counts determined in a gamma counter. Sera from patients with fascioliasis, taeniasis, schistosomiasis, oxyuriasis, trichinellosis and ancyclostomiasis gave counts similar to cord serum controls. Sera from patients with ascariasis gave counts of up to twice as great as controls, but sera from patients with toxicariasis produced counts of 7,000-13,000, a 4-6-fold increase.
- Subjects :
- Time Factors
Preservation, Biological
Immunology
Dose-Response Relationship, Immunologic
Schistosomiasis
Cross Reactions
Trichinosis
Radioimmunosorbent Test
Incubators
Dogs
Blood serum
Antigen
Antibody Specificity
medicine
Animals
Humans
Immunology and Allergy
Toxocara
Sheep
biology
Radioimmunoassay
medicine.disease
biology.organism_classification
Molecular biology
Larva
biology.protein
Toxocariasis
Female
Antibody
Toxocara canis
Subjects
Details
- ISSN :
- 00221759
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Journal of Immunological Methods
- Accession number :
- edsair.doi.dedup.....cefb94408aaa3787ffaa06baa61d0004
- Full Text :
- https://doi.org/10.1016/0022-1759(80)90180-5