1. Antioxidative natural product protect against econazole-induced liver injuries
- Author
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Chun Ching Lin, Song-Chow Lin, Yun Ho Lin, Chia Hsien Lin, Chin Fa Chen, Chao Kuang Lin, and Chi Feng Liu
- Subjects
Male ,Antifungal Agents ,Econazole ,Aspartate transaminase ,Pharmacology ,Toxicology ,Ferric Compounds ,Antioxidants ,Propolis ,Lipid peroxidation ,Biological Factors ,chemistry.chemical_compound ,Chlorides ,Liver Function Tests ,Superoxides ,medicine ,Animals ,Aspartate Aminotransferases ,Rats, Wistar ,IC50 ,Liver injury ,biology ,Chemistry ,Alanine Transaminase ,Free Radical Scavengers ,Oxidants ,medicine.disease ,Rats ,Liver ,Alanine transaminase ,Biochemistry ,Toxicity ,biology.protein ,Lipid Peroxidation ,Liver function ,Chemical and Drug Induced Liver Injury ,medicine.drug - Abstract
The study objective of this research is in order to investigate the hepatoprotective and therapeutic effects of propolis ethanol extract (PEE) on acute econazole-induced liver injury. Positive control of various concentrations of PEE on liver function and the dose-response relationship of liver injury induced by various doses of econazole were firstly observed from biochemical assay of serum level of aspartate transaminase (SGOT) and serum alanine transaminase (SGPT) and histopathological microscopic examination. The hepatoprotective effects of various concentration of PEE on liver damage induced by hepatotoxic dose (300 mg/kg) of econazole were observed by the obvious decrement of SGOT and SGPT level and further confirmed by hepatohistological microscopic examination. The inhibitory effects of PEE on FeCl2-induced (in vitro) or econazole-induced (in vivo) lipid peroxidation were investigated from the measurement of the formed malonic dialdehyde (MDA) level in the rat liver homogenate. The IC50 (μM) of various concentrations of PEE in the superoxide scavenging activity in econazole (300 mg/kg)-damaged rat liver homogenate were assessed by cytochrome c reduction method and compared with that of (+)-alpha-tocopherol. It could be postulated that the hepatoprotective effect of PEE may be, at least in part, due to their inhibitory ability on membrane lipid peroxidation and free radical formation or due to their free radical scavenging ability.
- Published
- 2004