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Hepatoprotective and therapeutic effects of tetramethylpyrazine on acute econazole-induced liver injury
- Source :
- Planta medica. 68(6)
- Publication Year :
- 2002
-
Abstract
- 2,3,5,6-Tetramethylpyrazine (TMP) is well known as a true calcium antagonist. The aim of this study was to investigate the hepatoprotective and therapeutic effects of TMP on acute econazole-induced liver injury. The hepatological effect of various concentrations of TMP was first assessed by the biochemical assays of SGOT and SGPT and then by hepatohistological microscopic examination. The dose-response relationship of liver injury induced by various doses of econazole was observed simultaneously from serum biochemical assay of SGOT and SGPT, and also from hepatohistological microscopic examination, by determination of the hepatoprotective effects of various concentrations of TMP on SGOT and SGPT elevation induced by a hepatotoxic dose of econazole (300 mg/kg). The inhibitory effect of various concentrations of TMP or vitamin E (positive control, 0.5 mM in vitro, 0.69 mM in vivo) on FeCl 2 -induced (in vitro) or econazole-induced (in vivo) lipid peroxidation was also investigated. The superoxide scavenging activity of various concentrations of TMP in econazole-damaged rat liver homogenate was assessed by the cytochrome C reduction method. Results showed that the hepatoprotective effect of TMP might be, at least in part, due to its inhibitory ability on membrane lipid peroxidation and free radical formation, or due to its free radical scavenging ability. Improvement of serum transaminases and MDA levels in rat liver homogenate, hepatohistological microscopic examination, and assessment of free radical scavenging activity by the cytochrome C reduction method were used to detect hepatoprotective and therapeutic effects of TMP on acute econazole-induced liver injury.
- Subjects :
- Male
Antioxidant
Econazole
medicine.medical_treatment
Pharmaceutical Science
Cytochrome c Group
Pharmacology
Analytical Chemistry
Lipid peroxidation
chemistry.chemical_compound
In vivo
Superoxides
Malondialdehyde
Drug Discovery
medicine
Tetramethylpyrazine
Animals
Vitamin E
Aspartate Aminotransferases
Ferrous Compounds
Rats, Wistar
Liver injury
biology
Dose-Response Relationship, Drug
Chemistry
Liver Diseases
Organic Chemistry
Alanine Transaminase
Free Radical Scavengers
medicine.disease
Rats
Complementary and alternative medicine
Biochemistry
Alanine transaminase
Liver
Pyrazines
Toxicity
Acute Disease
biology.protein
Molecular Medicine
Lipid Peroxidation
Chemical and Drug Induced Liver Injury
medicine.drug
Silymarin
Subjects
Details
- ISSN :
- 00320943
- Volume :
- 68
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Planta medica
- Accession number :
- edsair.doi.dedup.....645ff6fd41ede0fbf055033b8aa42268