84 results on '"Huitao Zhang"'
Search Results
2. Associations of the circulating levels of cytokines with the risk of myeloproliferative neoplasms: a bidirectional mendelian-randomization study
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Hao Xiong, Huitao Zhang, Jun Bai, Yanhong Li, Lijuan Li, and Liansheng Zhang
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Myeloproliferative neoplasms ,Mendelian randomization ,Inflammatory ,Cytokines ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Objective In the pathogenesis of myeloproliferative neoplasms (MPN), inflammation plays an important role. However, it is unclear whether there is a causal link between inflammation and MPNs. We used a bidirectional, two-sample Mendelian randomization (MR) approach to investigate the causal relationship between systemic inflammatory cytokines and myeloproliferative neoplasms. Methods A genome-wide association study (GWAS) of 8293 European participants identified genetic instrumental variables for circulating cytokines and growth factors. Summary statistics of MPN were obtained from a GWAS including 1086 cases and 407,155 controls of European ancestry. The inverse-variance-weighted method was mainly used to compute odds ratios (OR) and 95% confidence intervals (Cl). Results Our results showed that higher Interleukin-2 receptor, alpha subunit (IL-2rα) levels, and higher Interferon gamma-induced protein 10 (IP-10) levels were associated with an increased risk of MPN (OR = 1.36,95%CI = 1.03–1.81, P = 0.032; OR = 1.55,95%CI = 1.09–2.22, P = 0.015; respectively).In addition, Genetically predicted MPN promotes expression of the inflammatory cytokines interleukin-10 (IL-10) (BETA = 0.033, 95% CI = 0.003 ~ 0.064, P = 0.032) and monokine induced by interferon-gamma (MIG) (BETA = 0.052, 95% CI = 0.002–0.102, P = 0.043) and, on activation, normal T cells express and secrete RANTES (BETA = 0.055, 95% CI = 0.0090.1, P = 0.018). Conclusion Our findings suggest that cytokines are essential to the pathophysiology of MPN. More research is required if these biomarkers can be used to prevent and treat MPN.
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- 2024
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3. Grating-based x-ray dark-field CT for lung cancer diagnosis in mice
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Peiyuan Guo, Li Zhang, Jincheng Lu, Huitao Zhang, Xiaohua Zhu, Chengpeng Wu, Xiangwen Zhan, Hongxia Yin, Zhenchang Wang, Yan Xu, and Zhentian Wang
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Disease progression ,Early detection of cancer ,Lung diseases ,Tomography (x-ray computed) ,X-rays ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 - Abstract
Abstract Background The low absorption of x-rays in lung tissue and the poor resolution of conventional computed tomography (CT) limits its use to detect lung disease. However, x-ray dark-field imaging can sense the scattered x-rays deflected by the structures being imaged. This technique can facilitate the detection of small alveolar lesions that would be difficult to detect with conventional CT. Therefore, it may provide an alternative imaging modality to diagnose lung disease at an early stage. Methods Eight mice were inoculated with lung cancers simultaneously. Each time two mice were scanned using a grating-based dark-field CT on days 4, 8, 12, and 16 after the introduction of the cancer cells. The detectability index was calculated between nodules and healthy parenchyma for both attenuation and dark-field modalities. High-resolution micro-CT and pathological examinations were used to crosscheck and validate our results. Paired t-test was used for comparing the ability of dark-field and attenuation modalities in pulmonary nodule detection. Results The nodules were shown as a signal decrease in the dark-field modality and a signal increase in the attenuation modality. The number of nodules increased from day 8 to day 16, indicating disease progression. The detectability indices of dark-field modality were higher than those of attenuation modality (p = 0.025). Conclusions Compared with the standard attenuation CT, the dark-field CT improved the detection of lung nodules. Relevance statement Dark-field CT has a higher detectability index than conventional attenuation CT in lung nodule detection. This technique could improve the early diagnosis of lung cancer. Key points • Lung cancer progression was observed using x-ray dark-field CT. • Dark-field modality complements with attenuation modality in lung nodule detection. • Dark-field modality showed a detectability index higher than that attenuation in nodule detection. Graphical Abstract
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- 2024
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4. A spider mating plug functions to protect sperm.
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He Jiang, Yongjia Zhan, Qingqing Wu, Huitao Zhang, Matjaž Kuntner, and Lihong Tu
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Medicine ,Science - Abstract
Mating plugs in animals are ubiquitous and are commonly interpreted to be products of mating strategies. In spiders, however, mating plugs may also take on functions beyond female remating prevention. Due to the vagaries of female genital (spermathecal) anatomy, most spiders face the problem of having to secure additional, non-anatomical, protection for transferred sperm. Here, we test the hypothesis that mating plugs, rather than (or in addition to) being adaptations for mating strategies, may serve as sperm protection mechanism. Based on a comparative study on 411 epigyna sampled from 36 families, 187 genera, 330 species of entelegyne spiders, our results confirm the necessity of a sperm protection mechanism. We divided the entelegyne spermathecae into four types: SEG, SED, SCG and SCD. We also studied detailed morphology of epigynal tracts in the spider Diphya wulingensis having the SEG type spermathecae, using 3D-reconstruction based on semi thin histological series section. In this species, we hypothesize that two distinct types of mating plug, the sperm plug and the secretion plug, serve different functions. Morphological details support this: sperm plugs are formed on a modified spermathecal wall by the spilled sperm, and function as a temporary protection mechanism to prevent sperm from leaking and desiccating, while secretion plugs function in postcopulation both as a permanent protection mechanism, and to prevent additional mating. Furthermore, with the modified spermathecal wall of S2 stalk, the problem of shunt of sperm input and output, and the possibility of female multiple mating have been resolved. Variation in spermathecal morphology also suggests that the problem of sperm protection might be resolved in different ways in spiders. Considering mating plugs of varying shapes and origins in the vast morphospace of spiders, we conclude that mating plugs might serve different purposes that relate both to mating strategies, as well as to sperm protection.
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- 2024
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5. Fracture-vuggy carbonate reservoir characterization based on multiple geological information fusion
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Yan Wang, Pengfei Xie, Huitao Zhang, Yuming Liu, and Aiying Yang
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carbonate reservoir ,Fractured-vuggy reservoir ,geological modeling ,probability fusion ,fracture ,Science - Abstract
The complexity and strong heterogeneity of carbonate reservoirs with fracture-vuggy structures present significant challenges in reservoir characterization. To address these challenges, we propose a novel multi-element information fusion modeling approach. This approach is designed to integrate multiple methods and incorporate multi-probability fusion at various facies and scales, thereby bridging the gap between geological information and reservoir modeling. Our methodology involves four key steps. First, the statistics between frequency of karst and geological information are acquired, and we quantify the statistics to regression equations. Second, these regression equations are transferred to probability bodies. The probability bodies can be applied in modeling as a soft control. But just one single body can be input in modeling process. Third, multiple probability bodies are fused into a fusion probability body by a probability fusion algorithm, which can keep the potential information of probability bodies. Finally, we apply the probability body in modeling workflow. By this way, the fusion method bridges the gap between geological information and modeling. The model established through our proposed method showed a significant level of consistency with reservoir re-evaluation, achieving an impressive 90% degree of alignment. Furthermore, the history match analysis revealed a high correlation, indicating the model's reliability. The method effectively integrates various scales and types of geological information, offering an accurate approach to complex carbonate reservoir modeling.
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- 2024
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6. Mechanical stimulation on a microfluidic device to highly enhance small extracellular vesicle secretion of mesenchymal stem cells
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Rui Hao, Shi Hu, Huitao Zhang, Xi Chen, Zitong Yu, Jingyi Ren, Hang Guo, and Hui Yang
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Microfluidics ,Small extracellular vesicles ,Mechanical squeezing ,Secretion amount ,Mesenchymal stem cells ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Small extracellular vesicles (sEVs) are recognized as promising detection biomarkers and attractive delivery vehicles, showing great potential in diagnosis and treatment of diseases. However, the applications of sEVs are usually restricted by their poor secretion amount from donor cells under routine cell culture conditions, which is especially true for mesenchymal stem cells (MSCs) due to their limited expansion and early senescence. Here, a microfluidic device is proposed for boosting sEV secretion from MSCs derived from human fetal bone marrow (BM-MSCs). As the cells rapidly pass through a microfluidic channel with a series of narrow squeezing ridges, mechanical stimulation permeabilizes the cell membrane, thus promoting them to secrete more sEVs into extracellular space. In this study, the microfluidic device demonstrates that mechanical-squeezing effect could increase the secretion amount of sEVs from the BM-MSCs by approximately 4-fold, while maintaining cellular growth state of the stem cells. Further, the secreted sEVs are efficiently taken up by immortalized human corneal epithelial cells and accelerate corneal epithelial wound healing in vitro, indicating that this technique wound not affect the functionality of sEVs and demonstrating the application potentials of this technique.
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- 2023
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7. Optimization of Protoplast Preparation System from Leaves and Establishment of a Transient Transformation System in Apium graveolens
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Jiageng Du, Huitao Zhang, Weilong Li, Xiaoyan Li, Zhuo Wang, Ying Zhang, Aisheng Xiong, and Mengyao Li
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celery ,protoplast preparation ,transient transformation system ,subcellular localization ,Agriculture - Abstract
Protoplast culture and transformation technology offer a novel method for developing new plant varieties. Nonetheless, the effective preparation of protoplasts and transformation technology specific to celery has yet to be achieved. This study utilized celery seedling leaves as the primary materials to examine the key factors influencing protoplast isolation. The aim was to prepare leaf protoplasts with a high yield and of high quality and subsequently conduct transient gene transformation and expression. The findings indicated that the most effective procedure for isolating and purifying protoplasts was enzymatic digestion using an enzyme solution consisting of 2.0% cellulase, 0.1% pectolase, and 0.6 M mannitol for a duration of 8 h. Subsequently, the protoplasts were filtered through a 400-mesh sieve and purified through centrifugation at 200× g. Within this system, the overall protoplast yield was exceptionally high, reaching a viability rate of up to 95%. The transient transformation system yielded a maximum transformation efficiency of approximately 53%, as evaluated using the green fluorescent protein (GFP) as a reporter gene. The parameters of the transient transformation system were as follows: a protoplast concentration of 5 × 105 cells·mL−1, exogenous DNA concentration of 500 μg·mL−1, final concentration of PEG4000 at 40%, and transformation duration of 15 min. The transient transformation system was also utilized to further analyze the protein localization characteristics of the celery transcription factor AgMYB80. The findings indicated that AgMYB80 predominantly localizes in the nucleus, thereby confirming the reliability and effectiveness of the transient transformation system. This study successfully established an efficient system for isolating, purifying, and transforming celery protoplasts, and will serve as a basis for future studies on molecular biology and gene function.
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- 2023
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8. Chattering suppression of the sliding mode observer for marine electric propulsion motor based on piecewise power function
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Xiangfeng Li, Shengqiang Zhan, Fang Guo, Zidan Zhuang, Huitao Zhang, Hui Liao, and Lili Qu
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PMSM ,sliding mode observer ,sensorless control ,piecewise power function ,chatter suppression ,General Works - Abstract
The sliding mode observer (SMO)–based sensorless control is essentially a discontinuous switching control algorithm. Therefore, there is large speed or torque chattering in the permanent magnet synchronous motor (PMSM) with existing sliding mode observer–based control methods. In order to solve this problem well, an algorithm based on an improved sliding mode observer is proposed. In detail, the piecewise power function approach is used to replace the traditional ones, that is, the sgn function or the sigmoid function approach. Simulation verifications are conducted to validate the effectiveness of the proposed observer. It shows that the piecewise power function–based sliding mode observer algorithm has obvious advantages in the stability of the three-phase current, namely, it improves the estimation accuracy of the rotor position, rotor speed, and dynamic response to various loads. For further verification, the proposed sliding mode observer algorithm is implemented and verified in a marine electric propulsion motor with a DSP chip of TMS320F28377D. Experimental results validate that the piecewise power function–based sliding mode observer algorithm has high control accuracy and fewer fluctuations, which are all vital in marine engineering.
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- 2022
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9. Generation of three lines from multiorgan venous and lymphatic defect syndrome patients
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Xiaojun Hu, Junjie Mao, Ke Zhang, Huitao Zhang, Dan Li, Bin Zhou, Hong Shan, Bing Li, and Pengfei Pang
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Biology (General) ,QH301-705.5 - Abstract
Multiorgan venous and lymphatic defect (MOVLD) syndrome is a unique visceral vascular malformations with complex etiologies. In this study, primary skin fibroblasts were obtained from three MOVLD patients and reprogrammed into iPSCs by Yamanaka’s classical strategy. The MOVLD- iPSCs carrying the DDX24 p.Glu271Lys mutation were confirmed by Sanger sequencing. The pluripotency of MOVLD-iPSCs was verified by the specific molecular markers and gene expression, trilineage differentiation potential. The establishment of the MOVLD-iPSCs will provide a useful model for understanding the mechanisms involved the MOVLD and promoting the development of medical treatment.
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- 2022
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10. A finite element analysis of the impact of split pole shoes on magnetic liquid sealing performance
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Zhiguo Liu, Huitao Zhang, and Li Yang
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split magnetic liquid sealing ,split pole shoe ,magnetic sealant ,sealing performance ,finite element analysis ,Mathematics ,QA1-939 - Abstract
Though split pole shoe can solve the problems of increased cost and decreased efficiency caused by replacing pole shoes in the test, assembling and use of split sealing devices, it fails to overcome the issue of sealing in the gap between split pole shoe's junction surface and shaft. In this paper, a split magnetic liquid sealing structure is designed, combining magnetic liquid sealing and split pole shoe's plane sealing. In our study, we have adopted three methods for split sealing and use theory and simulation to ascertain the sealing performance of the split magnetic liquid sealing structure designed by us. The results indicate that magnetic sealant bonded split pole shoes show better sealing performance as compared to those bonded by ordinary sealant, and that the structural optimisation results can meet the requirement for sealing performance. The simulation results coincide with theoretical results, which proves the correctness of this research.
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- 2021
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11. Multi-Scale Dilated Convolution Neural Network for Image Artifact Correction of Limited-Angle Tomography
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Haichuan Zhou, Yining Zhu, Qian Wang, Jinqiu Xu, Ge Li, Defeng Chen, Yingying Dong, and Huitao Zhang
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Limited-angle tomography ,artifact correction ,multi-scale ,dilated convolution ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
Limited-angle computed tomography (CT) has arisen in some medical and industrial applications. It is also a challenging problem since some scan views are missing and the directly reconstructed images often suffer from severe distortions. For such kind of problems, we analyze the features of limited-angle CT images and propose a multi-scale dilated convolution neural network (MSD-CNN) to correct the artifacts and to restore the image. In this network, the dilated convolution layer and multi-scale pooling layer are combined to form a group and exited in the whole encoder-decoder process. Since the dilated convolutions support an exponential expansion of the receptive field without losing resolution and coverage, the obtained artifact features possess the multi-scale characteristic. Furthermore, to improve the effectiveness and accuracy of the training step, we employ a preprocessing method, which extracts image patches. Numerical experiments verify the out-performance of the proposed method compared with some conventional methods, such as Unet based deep learning,TV- and L0 -based optimization methods.
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- 2020
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12. CYP1A1 methylation mediates the effect of smoking and occupational polycyclic aromatic hydrocarbons co-exposure on oxidative DNA damage among Chinese coke-oven workers
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Yanli Liu, Xuejing Li, Bin Zhang, Ye Fu, Aimin Yang, Hongjie Zhang, Huitao Zhang, Yingying Niu, Jisheng Nie, and Jin Yang
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Oxidative DNA damage ,Polycyclic aromatic hydrocarbons ,Smoking ,Cytochrome P4501A1 ,DNA methylation ,Industrial medicine. Industrial hygiene ,RC963-969 ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background Multiple factors, including co-exposure between lifestyle and environmental risks, are important in susceptibility to oxidative DNA damage. However, the underlying mechanism is not fully understood. This study was undertaken to evaluate whether Cytochrome P4501A1 (CYP1A1) methylation can mediate the co-exposure effect between smoking and occupational polycyclic aromatic hydrocarbons (PAH) in development of oxidative DNA damage. Methods We explored the associations between smoking and occupational PAH co-exposure effect, CYP1A1 methylation and oxidative DNA damage among 500 workers from a coke-oven plant in China. Urine biomarkers of PAH exposure (1-hydroxypyrene, 1-OHP; 2-hydroxynaphthalene, 2-NAP; 2-hydroxyfluorene, 2-FLU; and 9-hydroxyphenanthren, 9-PHE) and a marker of oxidative DNA damage (8-hydroxy- 2′- deoxyguanosine, 8-OHdG) were measured by high performance liquid chromatography. CYP1A1 methylation was measured by pyrosequencing. Finally, mediation analysis was performed to investigate whether CYP1A1 methylation mediated smoking and occupational PAH co-exposure effect on oxidative DNA damage. Results We observed significant associations of smoking and 1-OHP co-exposure with CYP1A1 hypomethylation (OR: 1.87, 95% CI: 1.01–3.47) and high 8-OHdG (OR: 2.13, 95% CI: 1.14–3.97). There was a significant relationship between CYP1A1 hypomethylation and high 8-OHdG (1st vs. 3rd tertile = 1.58, 95% CI: 1.01–2.47, P for trend = 0.046). In addition, mediation analysis suggested CYP1A1 hypomethylation could explain 13.6% of effect of high 8-OHdG related to smoking and 1-OHP co-exposure. Conclusions Our findings suggested that the co-exposure effect of smoking and occupational PAH could increase the risk of oxidative DNA damage by a mechanism partly involving CYP1A1 hypomethylation.
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- 2019
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13. A method for material decomposition and quantification with grating based phase CT.
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Shiwo Deng, Yining Zhu, Huitao Zhang, Qian Wang, Peiping Zhu, Kai Zhang, and Peng Zhang
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Medicine ,Science - Abstract
Material decomposition (MD) is an important application of computer tomography (CT). For phase contrast imaging, conventional MD methods are categorized into two types with respect to different operation sequences, i.e., "before" or "after" image reconstruction. Both categories come down to two-step methods, which have the problem of noise amplification. In this study, we incorporate both phase and absorption (PA) information into MD process, and correspondingly develop a simultaneous algebraic reconstruction technique (SART). The proposed method is referred to as phase & absorption material decomposition-SART (PAMD-SART). By iteratively solving an optimization problem, material composition and substance quantification are reconstructed directly from absorption and differential phase projections. Comparing with two-step MD, the proposed one-step method is superior in noise suppression and accurate decomposition. Numerical simulations and synchrotron radiation based experiments show that PAMD-SART outperforms the classical MD method (image-based and dual-energy CT iterative method), especially for the quantitative accuracy of material equivalent atomic number.
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- 2021
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14. Design and Performance Simulation of Computer Control System for Automatic Monitoring of Upper Computer Communication Operation State
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Li Yang and Huitao Zhang
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Mechanical engineering and machinery ,TJ1-1570 - Abstract
The upper computer communication operation state automatic monitoring system is mainly used to remotely monitor the equipment, obtain various parameter indexes in the operation process of remote equipment, realize remote monitoring and fault diagnosis, and improve the management efficiency of decentralized equipment. This paper completes the design of communication, data storage, query, and other subsystems of upper and lower computers. The lower computer establishes a data channel with the OPC server through the MPI protocol and uploads the collected data to the OPC server in real time. The upper computer reads the data through the OPC server and displays the changes of monitored parameters in real time through the monitoring interface, so as to give an alarm under abnormal conditions. In addition, since the default database of Kingview is access, considering that the Microsoft Access database can store up to 2G of content, in order to upgrade and expand the subsequent system, SQL Server database is selected for data query, backup, and saving. The parameter setting method of communication control system is analyzed, the simulation model of industrial boiler control system is established by using Matlab/Simulink, and the interface between host computer software (IBCCS-e) and the model is provided. This paper analyzes the results of communication parameter adjustment. The simulation results show that the industrial boiler computer control system (IBCCS) has stable performance, low cost, convenient operation, and good maintainability. After further improvement, it has certain application value in the operation transformation of new small- and medium-sized boilers and original boilers.
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- 2021
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15. Comparative morphology refines the conventional model of spider reproduction.
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Yongjia Zhan, He Jiang, Qingqing Wu, Huitao Zhang, Zishang Bai, Matjaž Kuntner, and Lihong Tu
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Medicine ,Science - Abstract
Our understanding of spider reproductive biology is hampered by the vast anatomical diversity and difficulties associated with its study. Although authors agree on the two general types of female spider genitalia, haplogyne (plesiomorphic) and entelegyne (apomorphic), our understanding of variation within each group mostly concerns the external genital part, while the internal connections with the reproductive duct are largely unknown. Conventionally and simplistically, the spermathecae of haplogynes have simple two-way ducts, and those of entelegynes have separate copulatory and fertilization ducts for sperm to be transferred in and out of spermathecae, respectively. Sperm is discharged from the spermathecae directly into the uterus externus (a distal extension of the oviduct), which, commonly thought as homologous in both groups, is the purported location of internal fertilization in spiders. However, the structural evolution from haplo- to entelegyny remains unresolved, and thus the precise fertilization site in entelegynes is ambiguous. We aim to clarify this anatomical problem through a widely comparative morphological study of internal female genital system in entelegynes. Our survey of 147 epigyna (121 examined species in 97 genera, 34 families) surprisingly finds no direct connection between the fertilization ducts and the uterus externus, which, based on the homology with basal-most spider lineages, is a dead-end caecum in entelegynes. Instead, fertilization ducts usually connect with a secondary uterus externus, a novel feature taking over the functional role of the plesiomorphic uterus externus. We hypothesize that the transition from haplo- to entelegyny entailed not only the emergence of the two separate duct systems (copulatory, fertilization), but also involved substantial morphological changes in the distal part of the oviduct. Thus, the common oviduct may have shifted its distal connection from the uterus externus to the secondary uterus externus, perhaps facilitating discharge of larger eggs. Our findings suggest that the conventional model of entelegyne reproduction needs redefinition.
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- 2019
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16. Generation of Stable Cell Lines of APP695 (K595N, M596L) Gene Over-Expression via Lentiviral Over-Expression System
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Huitao Zhang, Hao, Rui, Yu, Zitong, Hu, Shi, Ren, Jingyi, Hong, Yanhang, Wang, Shifan, Sun, Huihui, Zhang, Pengcheng, Zhang, Yi, Yang, Hui, and Ding, Juan
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- 2024
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17. Available energy routing algorithm considering QoS requirements for LEO satellite network.
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Li Yang, Huitao Zhang, Yaowen Qi, and Qilong Huang
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- 2024
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18. One-Step Method for Material Quantitation Using In-Line Tomography With Single Scanning.
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Suyu Liao, Shiwo Deng, Yining Zhu, Huitao Zhang, Peiping Zhu, Kai Zhang, and Xing Zhao
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- 2023
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19. Design and implementation of open laboratory management system based on 2DPCA face identification technology.
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Huitao Zhang, Li Yang, Jingwu Sun, and Zhiguo Liu
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- 2022
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20. Protein Post-translational Modification Site Prediction using Deep Learning.
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Yujuan Deng, Yunfang Fu, Huitao Zhang, Xin Liu, and Zhiguo Liu
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- 2021
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21. Enhancing the X-Ray Differential Phase Contrast Image Quality With Deep Learning Technique.
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Yongshuai Ge, Peizhen Liu, Yifan Ni, Jianwei Chen, Jiecheng Yang, Ting Su, Huitao Zhang, Jinchuan Guo, Hairong Zheng, Zhicheng Li 0001, and Dong Liang 0001
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- 2021
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22. Fast Iterative Reconstruction for Multi-spectral CT by a Schmidt Orthogonal Modification Algorithm (SOMA).
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Huiying Pan, Shusen Zhao, Weibin Zhang, Huitao Zhang, and Xing Zhao
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- 2022
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23. Influence of different microbial inoculants on nitrogen retention and diazotroph community succession during cotton straw composting
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Liuyan Zhou, Yuqing Xie, Xiaowu Wang, Pengbing Li, Yuyue Liu, Zhifang Wang, Jinping Dai, Huitao Zhang, and Xinping Yang
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Environmental Engineering ,General Chemical Engineering ,Environmental Chemistry ,Safety, Risk, Reliability and Quality - Published
- 2023
24. Nitratireductor luteus sp. nov. isolated from saline-alkali land
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Xinping, Yang, Liuyan, Zhou, Xiaowu, Wang, Zhifang, Wang, Pengbin, Li, Jinping, Dai, Huitao, Zhang, and Yuqing, Xie
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General Medicine ,Molecular Biology ,Microbiology - Abstract
The Gram-staining negative, oxidase and catalase negative strain KC-ST17
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- 2022
25. Hemodialysis Arteriovenous Fistula Dysfunction: Retrospective Comparison of Post-thrombotic Percutaneous Endovascular Interventions with Pre-emptive Angioplasty
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Xinyan Hu, Bing Li, Junjie Mao, Xiaojun Hu, Jingjing Zhang, Hui Guo, Dashuai Wang, Yongyu Zhang, Jianan He, Ni Zhao, Huitao Zhang, and Pengfei Pang
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Angioplasty ,Graft Occlusion, Vascular ,Thrombosis ,General Medicine ,Urokinase-Type Plasminogen Activator ,Arteriovenous Shunt, Surgical ,Treatment Outcome ,Renal Dialysis ,Arteriovenous Fistula ,Humans ,Surgery ,Cardiology and Cardiovascular Medicine ,Angioplasty, Balloon ,Vascular Patency ,Retrospective Studies - Abstract
We aimed to compare the clinical outcomes of pre-emptive angioplasty versus post-thrombotic percutaneous endovascular restoration of dysfunctional arteriovenous fistula (AVF).This retrospective study reviewed the data from 80 patients who underwent 114 endovascular interventions for a malfunctioning AVF from July 2016 to August 2019. Stenotic AVFs were treated with pre-emptive angioplasty. Thrombosed AVFs were treated with percutaneous pharmacomechanical fibrinolysis with urokinase used only during the operation or continuously infused. The differences in patency rates were evaluated using the Kaplan-Meier method. In addition, univariate and multivariate regression Cox models were used to determine influential factors on the postintervention primary patency.Post-thrombotic interventions and pre-emptive angioplasty yielded statistically similar rates in clinical success (100 vs. 100%), anatomic success (94 vs. 89%; P = 0.52), complication (4 vs. 11%; P = 0.29), as well as postintervention primary, assisted primary and secondary patency (P = 0.80; 0.57; 0.57). The use of pre-emptive angioplasty was associated with reduced total cost (¥25,108 vs. ¥30,833, P 0.001). The patients who used urokinase only during the operation prolonged both the primary and assisted primary patency (P = 0.02; 0.002), while those with continuous infusion of urokinase had worst patency rates and high costs (¥39,275 vs. ¥25,108 vs. ¥27,140, P 0.001). Compared with the other locations, dysfunction in the anastomotic or juxta-anastomotic segment (HR = 0.41, P = 0.001) was associated with prolonged postintervention primary patency.No clinical outcome differences were found between the post-thrombotic percutaneous endovascular interventions and pre-emptive angioplasty. However, pre-emptive angioplasty decreased access expenditure.
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- 2022
26. Effects of bacterial inoculation on lignocellulose degradation and microbial properties during cow dung composting.
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Liuyan Zhou, Xinping Yang, Xiaowu Wang, Lei Feng, Zhifang Wang, Jinping Dai, Huitao Zhang, and Yuqing Xie
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- 2023
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27. Reconstruction Algorithm-Based CT Imaging for the Diagnosis of Hepatic Ascites
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Huitao Zhang, Wenhao Lv, Haofeng Diao, and Li Shang
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Article Subject ,General Immunology and Microbiology ,Applied Mathematics ,Ascites ,Water ,General Medicine ,General Biochemistry, Genetics and Molecular Biology ,Artificial Intelligence ,Modeling and Simulation ,Humans ,Radiographic Image Interpretation, Computer-Assisted ,Calcium ,Tomography, X-Ray Computed ,Algorithms ,Iodine - Abstract
The study was aimed at exploring the diagnostic value of artificial intelligence reconstruction algorithm combined with CT image parameters on hepatic ascites, expected to provide a reference for the etiological evaluation of clinical abdominal effusion. Specifically, the adaptive iterative hard threshold (AIHT) algorithm for CT image reconstruction was proposed. Then, 100 patients with peritoneal effusion were selected as the research subjects. After 8 cases were excluded, the remaining was divided into 50 cases of the S1 group (hepatic ascites) and 42 cases of the D0 group (cancerous peritoneal effusion). Gemstone energy spectrum CT scanning was performed on all patients, and CT image parameters of the two groups were compared. It was found that CT value of mixed energy, CT value of 60-100 KeV single energy, concentration value of water (calcium), concentration value of water (iodine), and slope of energy spectrum curve in the S1 group were significantly lower than those in the D0 group ( P < 0.05 ). The effective atomic number in the S1 group was significantly higher than that in the D0 group ( P < 0.05 ). Of the 50 patients in the S1 group, 3 (6%) had an ascending and 47 (94%) had a descending spectral curve. Of the 42 patients in the D0 group, 37 (88.1%) had an ascending and 5 (11.9%) had a descending spectral curve. The sensitivity and specificity of water (iodine) were 0.927 and 0.836, respectively. The sensitivity and specificity of water (calcium) were 0.863 and 0.887, respectively. For different scan ranges ([0,90]; [0,120]), root mean square error (RMSE) of AIHT reconstructed image was significantly smaller than that of traditional algorithm, while peak signal-to-noise ratio (PSNR) was opposite. The differences were statistically significant ( P < 0.05 ). In conclusion, AIHT-based CT images can better display the distribution of hepatic ascites, and the parameters of CT value, effective atomic number, water (iodine), water (calcium), and spectral curve can all provide help for the identification of hepatic ascites. Especially, water (iodine) and water (calcium) demonstrated high diagnostic performance of hepatic ascites.
- Published
- 2022
28. Analysis of Spatial Structure and Filling Mechanism of Paleokarst Conduit Against a Background of Denudation: A Case Study in the Tahe Oilfield, Tarim Basin
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Chenhe Zhang, Zhiqian Gao, Tailiang Fan, Duan Wei, Jun Wua, Debin Yang, Jinxian Liu, Huitao Zhang, Bo Li, Zhe Chang, Yu Gu, Ming Jiang, Ze Gao, Aolin Wang, and Qianxi Zou
- Published
- 2023
29. A GPU-based multi-resolution approach to iterative reconstruction algorithms in x-ray 3D dual spectral computed tomography.
- Author
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Jingjing Hu, Xing Zhao, and Huitao Zhang
- Published
- 2016
- Full Text
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30. Protein Post-translational Modification Site Prediction using Deep Learning
- Author
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Yujuan Deng, Yunfang Fu, Huitao Zhang, Xin Liu, and Zhiguo Liu
- Subjects
General Earth and Planetary Sciences ,General Environmental Science - Published
- 2022
31. Encapsulating has-miR-29a into HuMSCs-derived Exosomes by a Nanoengineering Platform to Efficiently Alleviate Aβ1-42 Expression in APPswe-SH-SY5Y Cells
- Author
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Huitao Zhang, Hui Yang, Rui Hao, Zitong Yu, Shi Hu, Jingyi Ren, Juan Ding, and Yi Zhang
- Abstract
Background Exosomes derived from human umbilical cord mesenchymal stem cells (HuMSCs) have been utilized as a potential therapeutic candidate to alleviate amyloid β-protein (Aβ) expression in APP/PS1 or 5XFAD transgenic mice due to their low immunogenicity and extensive sources. Accumulated evidence also suggests that encysting appropriate cargos into exosomes can more effectively reduce Aβ expression by interfering with the metabolism of β-Amyloid precursor protein (APP). Therefore, exosomes loaded with bioactive substances to reduce Aβ expression have attracted much attention. A new strategy to load therapeutic exogenous cargoes of interest into exosomes in a high-throughput and straightforward fashion is desired. Results In this study, neuroblastoma SH-SY5Y cells were stably transfected with Swedish mutant APP695K, 595N plasmid to construct a cell model of Aβ overexpression. Then extracted Exosomes from HuMSCs and loaded with micro (miR)-29a via a microfluidic exosome nanoengineering platform (ExoNP) to target a post-transcriptional modification of the β-secretase1 (β-site APP cleaving enzyme 1, BACE1) gene to prevent protein expression. Finally, processed exosomes were utilized to treat APPswe-SH-SY5Y cells and decrease the expression of Aβ1−42 and BACE1 and further alleviated apoptosis. Conclusion HuMSCs-derived exosomes loaded with hsa-miR-29a via ExoNP to efficiently deliver to APPswe-SH-SY5Y cells and alleviate Aβ1−42 expression and promote cell survival.
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- 2022
32. One-Step Method for Material Quantitation Using In-Line Tomography With Single Scanning
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Suyu Liao, Shiwo Deng, Yining Zhu, Huitao Zhang, Peiping Zhu, Kai Zhang, and Xing Zhao
- Subjects
Image and Video Processing (eess.IV) ,FOS: Electrical engineering, electronic engineering, information engineering ,Biomedical Engineering ,FOS: Physical sciences ,Medical Physics (physics.med-ph) ,Electrical Engineering and Systems Science - Image and Video Processing ,Physics - Medical Physics - Abstract
Objective: Quantitative technique based on In-line phase-contrast computed tomography with single scanning attracts more attention in application due to the flexibility of the implementation. However, the quantitative results usually suffer from artifacts and noise, since the phase retrieval and reconstruction are independent ("two-steps") without feedback from the original data. Our goal is to develop a method for material quantitative imaging based on a priori information specifically for the single-scanning data. Method: An iterative method that directly reconstructs the refractive index decrement delta and imaginary beta of the object from observed data ("one-step") within single object-to-detector distance (ODD) scanning. Simultaneously, high-quality quantitative reconstruction results are obtained by using a linear approximation that achieves material decomposition in the iterative process. Results: By comparing the equivalent atomic number of the material decomposition results in experiments, the accuracy of the proposed method is greater than 97.2%. Conclusion: The quantitative reconstruction and decomposition results are effectively improved, and there are feedback and corrections during the iteration, which effectively reduce the impact of noise and errors. Significance: This algorithm has the potential for quantitative imaging research, especially for imaging live samples and human breast preclinical studies.
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- 2022
33. The effect of bacteria inoculation on the lignocellulose degradation and the microbial properties during cow dung composting
- Author
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Liuyan Zhou, Xinping Yang, Yuqing Xie, Xiaowu Wang, Jinping Dai, Zhifang Wang, Huitao Zhang, and Lei Feng
- Abstract
The goal of this work was to explore the potential effects of bacteria inoculation on the lignocellulose degradation and the underlying microbial mechanism during cow dung composting. The results suggested that bacteria inoculation better-accelerated temperature rose and increased thermophilic phase. Compared to the control (without inoculation, CK), the cellulose, hemicellulose, and lignin degradation rates of treated group (EG) increased from 53.3% to 70.0%, 50.2% to 61.3% and 46.4% to 60.0%, respectively. The microbial community structure and diversity were obviously changed by inoculation. Moreover, inoculation contributed to modulate the key compost microbial functional populations linking to the degradation of lignocelluloses by stamp analysis. Correlation matrix analysis indicated that the expression of bacterial lingo-cellulolytic enzymes were closely related to the key microbial functional populations. Overall, the results confirmed the importance of bacteria inoculation and have important implication for promoting the efficiency and quality of cow dung compost.
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- 2022
34. MALDI-MS-based biomarker analysis of extracellular vesicles from human lung carcinoma cells
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Chao Zhao, Wei Li, Zitong Yu, Qian Luo, Renjie Zhang, Huitao Zhang, Hui Yang, and Shi Hu
- Subjects
0303 health sciences ,biology ,Cell growth ,Chemistry ,General Chemical Engineering ,010401 analytical chemistry ,S100A10 ,General Chemistry ,medicine.disease ,01 natural sciences ,RPS27A ,0104 chemical sciences ,Ribosome assembly ,03 medical and health sciences ,Cell culture ,Cancer cell ,biology.protein ,medicine ,Cancer research ,Lung cancer ,Fetal bovine serum ,030304 developmental biology - Abstract
Extracellular vesicles (EVs) are actively secreted by mammalian cells. They are increasingly recognized as promising circulating biomarkers of disease progression. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is currently one of the most powerful techniques for the rapid analysis of biological samples, especially for discovering biomarkers for disease diagnosis and prognosis. It is unclear what cell culture medium components and EV isolation methods are suitable for MALDI-TOF MS analysis. Using a human lung carcinoma cell line (A549), we investigated and optimized the critical experimental conditions for EVs' protein profiling by combining differential ultracentrifugation and MALDI-TOF MS. The results demonstrated that medium components and ultracentrifugation procedures to extract EVs played important roles in MS detection. Compared with EV-depleted serum and normal serum medium, conditioned medium with 2% fetal bovine serum in this study maintained cell proliferation and displayed significant protein profiling of EVs. RPS27A (ribosomal protein), which plays an essential role in mRNA translation and ribosome assembly for the differentiation of cancer cells, was detected from the EVs of lung cancer cells associated with cancer cell migration and invasion. We also found the known tumor diagnosis marker, which is S100A10_S100 calcium-binding protein A10. Therefore, MALDI-TOF MS-based EV analysis with optimized experimental protocols can contribute to future development of rapid screening techniques of protein biomarkers associated with early cancer diagnosis.
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- 2021
35. Multi-scale dilated dense reconstruction network for limited-angle computed tomography
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Haichuan Zhou, Yining Zhu, Huitao Zhang, Xing Zhao, and Peng Zhang
- Subjects
Radiological and Ultrasound Technology ,Radiology, Nuclear Medicine and imaging - Abstract
Objective. Due to the incomplete projection data, the image reconstructed by limited-angle computed tomography (CT) usually suffers from significant artifacts, by which the structural details are heavily blurred. In this study, we aim to develop a novel approach to improve the limited-angle CT reconstruction performance, especially for the narrow scanning angular range. Approach. A deep learning based iterative framework for limited-angle tomography is proposed, which is named multi-scale dilated dense reconstruction network (MSDDRNet). The MSDDRNet utilizes a multi-scale dilated dense convolution neural network (MSDD-CNN) with conventional reconstruction algorithm for predicting image from incomplete projection data. The MSDD-CNN enhances the image features in the network by merging the DenseNet-Like structure, which serves to restore invisible singularities and reduce artifacts, as well as introducing constraints on the projection domain data into the iterative process to achieve better image detail recovery. Additionally, to improve the training speed of the network, we use a strategy of pre-training and model migration. Main results. Numerical experiments demonstrate that the proposed MSDDRNet performs well in terms of artifact correction, noise reduction and structure recovery compared to existing methods with limited scan angles, and we also extend the proposed method to more general scanning condition and other application such as dental CT data. Significance. The proposed method is a general framework, which can be applied to other CT problems, such as low dose CT, sparse-data CT and spectral CT.
- Published
- 2023
36. Design of Anti-Lock Braking System for FSAE Racing Vehicle Based on New Slip Ratio Observation Method
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Yicai Liu, Da Wang, Luxu Liang, Huitao Zhang, Wenjie Wang, Mo Lv, Yiyang Wang, Yi Sun, and Shuai Yang
- Published
- 2022
37. Effect of SARS-CoV-2 Infection on the Microbial Composition of Upper Airway
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Changli Tu, Xiaobin Zheng, Huitao Zhang, Jing Liu, Pengfei Pang, Yingjian Liang, Zhenguo Wang, Yiming Wang, Xiaojun Hu, Hong Shan, and Zhonghe Li
- Subjects
0301 basic medicine ,Pharmacology ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Opportunistic infection ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,030106 microbiology ,Microbial composition ,macromolecular substances ,Biology ,medicine.disease ,medicine.disease_cause ,biology.organism_classification ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Infectious Diseases ,medicine ,Pharmacology (medical) ,030212 general & internal medicine ,Airway ,Bacteria ,Coronavirus - Abstract
Methods Forty-four COVID-19 patients (severe/critical: N = 8, non-severe: N = 36) were examined by next generation sequencing (NGS) of nasopharyngeal test paper to observe the effect of novel coronavirus infection to the microbial composition in upper airway. Results In these nasopharyngeal test paper samples, 38 kinds of bacteria, 10 kinds of viruses except SARS-CoV-2, nine kinds of fungi and three kinds of atypical pathogens had been found. There was some difference in microbial composition in the upper airway between severe and non-severe cases. Summary These results are important for us to study the effect of SARS-CoV-2 on the local microbial composition of upper airway and prevent opportunistic infection in severe patients.
- Published
- 2020
38. Synthesis of MnO2 modified porous carbon spheres by preoxidation-assisted impregnation for catalytic oxidation of indoor formaldehyde
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Changming Zhang, Caimei Fan, Huitao Zhang, Rui Li, Yaqi Wang, Xiaochao Zhang, and Song Wen
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Materials science ,Scanning electron microscope ,Mechanical Engineering ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Catalysis ,Adsorption ,X-ray photoelectron spectroscopy ,Chemical engineering ,Catalytic oxidation ,Mechanics of Materials ,Desorption ,General Materials Science ,Fourier transform infrared spectroscopy ,Temperature-programmed reduction ,0210 nano-technology - Abstract
Resin-based porous carbon spheres with well dispersed MnO2 particles were sucessfully synthesised by steam activation and preoxidation-assisted impregnation of manganese nitrate salt. X-ray diffractometer (XRD), Fourier Transform Infrared spectroscopy (FT-IR), N2 adsorption–desorption, scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), H2 temperature programmed reduction (H2-TPR), O2 temperature-programmed desorption (O2-TPD) and in-situ diffuse reflectance fourier transform spectrometry (DRIFTS) were applied to characterize the obtained porous carbon spheres, and the HCHO adsorption and stability performances were evaluated in a fixed bed reactor. The results demonstrated that surface chemical properties and HCHO removal performances were significantly enhanced after the modification of preoxidation-assisted impregnation. The optimal manganese nitrate loading value, and relative humidity were 6 and 50%, respectively. Moreover, the higher HCHO concentration showed a smaller breakthrough time. The HCHO removal efficiency of ACS–O-6% Mn remained 100% even after reaction for 20 h at room temperature, while the average HCHO removal efficiency only declines by 0.2% compared with the first adsorption after 10 regeneration cycles. The in-situ DRIFTS results showed the smaller accumulation and faster desorption of intermediate products over the ACS–O-6% Mn. The HCHO removal mechanism analysis indicated that the enriched Mn3+ and surface active chemisorbed oxygen accounted for the excellent catalytic oxidation activity and stability.
- Published
- 2020
39. Multi-Scale Dilated Convolution Neural Network for Image Artifact Correction of Limited-Angle Tomography
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Yining Zhu, Huitao Zhang, Yingying Dong, Jinqiu Xu, Qian Wang, Haichuan Zhou, Ge Li, and Defeng Chen
- Subjects
0209 industrial biotechnology ,General Computer Science ,Computer science ,multi-scale ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Scale (descriptive set theory) ,artifact correction ,02 engineering and technology ,Convolutional neural network ,Image (mathematics) ,Convolution ,020901 industrial engineering & automation ,0202 electrical engineering, electronic engineering, information engineering ,Preprocessor ,General Materials Science ,Computer vision ,Artifact (error) ,business.industry ,Deep learning ,General Engineering ,Process (computing) ,dilated convolution ,Limited-angle tomography ,020201 artificial intelligence & image processing ,Artificial intelligence ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,business ,lcsh:TK1-9971 - Abstract
Limited-angle computed tomography (CT) has arisen in some medical and industrial applications. It is also a challenging problem since some scan views are missing and the directly reconstructed images often suffer from severe distortions. For such kind of problems, we analyze the features of limited-angle CT images and propose a multi-scale dilated convolution neural network (MSD-CNN) to correct the artifacts and to restore the image. In this network, the dilated convolution layer and multi-scale pooling layer are combined to form a group and exited in the whole encoder-decoder process. Since the dilated convolutions support an exponential expansion of the receptive field without losing resolution and coverage, the obtained artifact features possess the multi-scale characteristic. Furthermore, to improve the effectiveness and accuracy of the training step, we employ a preprocessing method, which extracts image patches. Numerical experiments verify the out-performance of the proposed method compared with some conventional methods, such as Unet based deep learning,TV- and L0 -based optimization methods.
- Published
- 2020
40. Generation and phenotype analysis of CysLTR1 L118F mutant mice
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Huitao Zhang, Bing Li, Junjie Mao, Xiaojun Hu, Hong Shan, Bin Zhou, and Pengfei Pang
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Male ,0301 basic medicine ,Immunoprecipitation ,Mutant ,Biochemistry ,Calcium in biology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Animals ,Protein Interaction Domains and Motifs ,Molecular Biology ,Microinjection ,Receptors, Leukotriene ,Messenger RNA ,Behavior, Animal ,Chemistry ,Myocardium ,Point mutation ,Cell Biology ,Phenotype ,Cell biology ,Mice, Inbred C57BL ,Cysteinyl leukotriene receptor 1 ,030104 developmental biology ,030220 oncology & carcinogenesis ,Mutation ,Calcium ,Female ,Mutant Proteins - Abstract
Cysteinyl leukotrienes (CysLTs) are a group of eicosanoids that regulate the pathogenesis of various human diseases, mainly by signaling through the cysteinyl leukotriene receptor 1 (CysLTR1). The aim of this study was to generate and examine the phenotype of CysLTR1 L118F mutant mice. CysLTR1 L118F mutant mice were generated by the simultaneous microinjection of single guide RNA, Cas9 messenger RNA, and donor plasmid into fertilized mouse eggs. The morphological and behavioral characteristics of the resultant CysLTR1 L118F mutant mice were analyzed using an animal phenotype analysis platform, which included the assessment of body length, tail length, grip strength, and locomotor activity. Immunoprecipitation coupled with mass spectrometry was performed to identify CysLTR1-interacting proteins, and the intracellular calcium levels were determined using fluorometric imaging plate reader assays. The body length and tail length of CysLTR1 L118F mutant mice were significantly increased compared with wild-type mice. In addition, the grip strength and locomotor activity were remarkably elevated in L118F mutant mice compared with wild-type mice. Only three proteins were found to interact with both wild-type and CysLTR1 L118F proteins, whereas 4 and 13 additional proteins interacted exclusively with wild-type and mutant CysLTR1, respectively. Lastly, the responsiveness of cardiac muscle cells to CysLTs were significantly impaired by the L118F substitution in CysLTR1 proteins. The CysLTR1 L118F point mutation induced significant changes in the mouse morphology and behavior, which might be mediated by alterations of its protein interaction profile.
- Published
- 2019
41. Olmesartan Medoxomil, An Angiotensin II-Receptor Blocker, Ameliorates Renal Injury In db/db Mice
- Author
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Ye Zhu, Ze-Liang Li, Weiping Zhu, Tongxia Cui, Huitao Zhang, Ao Ding, Hua Zhang, and Hui Yang
- Subjects
0301 basic medicine ,Pharmacology ,medicine.medical_specialty ,Kidney ,Angiotensin receptor ,business.industry ,Pharmaceutical Science ,Renal function ,medicine.disease ,Streptozotocin ,Angiotensin II ,Diabetic nephropathy ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Endocrinology ,030220 oncology & carcinogenesis ,Internal medicine ,Diabetes mellitus ,Drug Discovery ,Medicine ,business ,Olmesartan ,medicine.drug - Abstract
Background Diabetic nephropathy (DN) is a common complication of diabetes mellitus (DM) and also a major cause of end-stage renal disease (ESRD). Olmesartan medoxomil (OM) is an angiotensin II receptor blocker (ARB) and has been shown to exhibit renoprotective effects on a streptozotocin (STZ)-induced diabetic rat model. Yet, whether OM affects DN progression and renal injury in db/db mice, a type 2 diabetic murine model, has not been established. Methods Wild-type (n = 15) and db/db mice (n = 15) were treated with control saline or OM via oral gavage. The physiological and biochemical parameters were evaluated and histological examinations of kidney specimens were performed. Results Compared with saline-treated db/db mice, db/db mice administered with OM showed ameliorated diabetic physiological and biochemical parameters. In addition, OM decreased urinary albumin excretion and plasma creatinine level in db/db mice. Moreover, histologically, OM reduced glomerular hypertrophy and injury, and also ameliorated tubular injury, thus suggesting that OM improves renal function and minimizes renal pathological deterioration in db/db mice. Conclusion Our study reveals a beneficial role of OM in ameliorating DN in db/db mice, which is associated with its renoprotective function.
- Published
- 2019
42. Generation of three lines from multiorgan venous and lymphatic defect syndrome patients
- Author
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Xiaojun Hu, Junjie Mao, Ke Zhang, Huitao Zhang, Dan Li, Bin Zhou, Hong Shan, Bing Li, and Pengfei Pang
- Subjects
DEAD-box RNA Helicases ,QH301-705.5 ,Induced Pluripotent Stem Cells ,Mutation ,Humans ,Cell Differentiation ,Cell Biology ,General Medicine ,Syndrome ,Biology (General) ,Fibroblasts ,Developmental Biology - Abstract
Multiorgan venous and lymphatic defect (MOVLD) syndrome is a unique visceral vascular malformations with complex etiologies. In this study, primary skin fibroblasts were obtained from three MOVLD patients and reprogrammed into iPSCs by Yamanaka’s classical strategy. The MOVLD- iPSCs carrying the DDX24 p.Glu271Lys mutation were confirmed by Sanger sequencing. The pluripotency of MOVLD-iPSCs was verified by the specific molecular markers and gene expression, trilineage differentiation potential. The establishment of the MOVLD-iPSCs will provide a useful model for understanding the mechanisms involved the MOVLD and promoting the development of medical treatment.
- Published
- 2021
43. Angular sensitivity of an x-ray differential phase contrast imaging system with real and virtual source images
- Author
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Huitao Zhang, Peiping Zhu, Yongshuai Ge, Dong Liang, Hairong Zheng, Jianwei Chen, and Jiecheng Yang
- Subjects
Physics ,business.industry ,Phase contrast microscopy ,X-ray ,Phase-contrast imaging ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,Virtual source ,law.invention ,010309 optics ,Optics ,law ,Thin lens ,0103 physical sciences ,Astronomical interferometer ,Sensitivity (control systems) ,0210 nano-technology ,business ,Differential phase contrast - Abstract
In this work, a novel, to the best of our knowledge, approach based on an x-ray thin lens imaging theory is proposed to predict the angular sensitivity responses of dual-phase-grating differential phase contrast (DPC) interferometers. Experimental validations have been performed to demonstrate the high accuracy of theoretical predictions using two different setups: one with real source images and the other with virtual source images. This new sensitivity calculation method is helpful to optimize the DPC imaging performance of a dual-phase-grating system.
- Published
- 2021
44. New troodontid theropod specimen from Inner Mongolia, China clarifies phylogenetic relationships of later-diverging small-bodied troodontids and paravian body size evolution
- Author
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Huitao Zhang, Qigao Jiangzuo, Lin Tan, Shuo Wang, Qingwei Tan, and Qiyue Zhang
- Subjects
China ,Phylogenetic tree ,Evolutionary biology ,Animals ,Body Size ,Bayes Theorem ,Body size ,Biology ,Inner mongolia ,Ecology, Evolution, Behavior and Systematics ,Phylogeny ,Dinosaurs - Abstract
A new troodontid (LH PV39) recovered from the Upper Cretaceous Wulansuhai Formation, Inner Mongolia, China, is described, highlighting the dorsoventrally compressed sacral centra. The completely fused neurocentral junctions indicate that LH PV39 had reached adulthood at the time of death, but its size is nevertheless 20% smaller than that of the sympatric Philovenator, demonstrating that it is the second small-bodied troodontid recovered from the Wulansuhai Formation. Phylogenetic analyses scoring LH PV39 using different strategies and performed with different algorithms unambiguously recovered it as a troodontid. While the parsimony-based analysis scoring LH PV39 as an independent OTU with all of its available characteristics included recovered it as a basal troodontid, the Bayesian analysis suggests a closer relationship of LH PV39 to Almas and an unnamed troodontid from Ukhaa Tolgod, Mongolia (MPC-D100/1126+D100/3500). Body size analysis confirmed a single trend of gigantism throughout the evolution of troodontids, and suggests that the Late Cretaceous troodontids evolved in two directions: (i) several size-independent characteristics evolved while retaining the small sizes that are typical of the Early Cretaceous relatives, resulting in the Late Cretaceous small-bodied troodontids; and (ii) size-dependent characteristics (e.g., the elongation of the rostrum) evolved accompanying the size increase, resulting in large-bodied derived troodontids. The mosaic features of the Late Cretaceous small-bodied troodontids place them intermediate between their Early Cretaceous basal relatives and the Late Cretaceous large-bodied taxa in a well-resolved phylogeny, which is crucial for understanding the size and morphological evolution of troodontids.
- Published
- 2021
45. CYP1A1 methylation mediates the effect of smoking and occupational polycyclic aromatic hydrocarbons co-exposure on oxidative DNA damage among Chinese coke-oven workers
- Author
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Bin Zhang, Yingying Niu, Xuejing Li, Jisheng Nie, Ye Fu, Hongjie Zhang, Huitao Zhang, Aimin Yang, Jin Yang, and Yanli Liu
- Subjects
Adult ,Male ,China ,medicine.medical_specialty ,Coke oven ,Health, Toxicology and Mutagenesis ,Oxidative dna damage ,03 medical and health sciences ,chemistry.chemical_compound ,lcsh:RC963-969 ,Occupational Exposure ,Internal medicine ,Cytochrome P-450 CYP1A1 ,medicine ,polycyclic compounds ,Humans ,Deoxyguanosine ,Cytochrome P4501A1 ,0303 health sciences ,DNA methylation ,business.industry ,Research ,lcsh:Public aspects of medicine ,Smoking ,Public Health, Environmental and Occupational Health ,030311 toxicology ,lcsh:RA1-1270 ,Methylation ,Middle Aged ,Pah exposure ,Polycyclic aromatic hydrocarbons ,Oxidative Stress ,Cross-Sectional Studies ,Endocrinology ,chemistry ,Oxidative DNA damage ,lcsh:Industrial medicine. Industrial hygiene ,Pyrosequencing ,Female ,Co exposure ,business ,DNA Damage - Abstract
Background Multiple factors, including co-exposure between lifestyle and environmental risks, are important in susceptibility to oxidative DNA damage. However, the underlying mechanism is not fully understood. This study was undertaken to evaluate whether Cytochrome P4501A1 (CYP1A1) methylation can mediate the co-exposure effect between smoking and occupational polycyclic aromatic hydrocarbons (PAH) in development of oxidative DNA damage. Methods We explored the associations between smoking and occupational PAH co-exposure effect, CYP1A1 methylation and oxidative DNA damage among 500 workers from a coke-oven plant in China. Urine biomarkers of PAH exposure (1-hydroxypyrene, 1-OHP; 2-hydroxynaphthalene, 2-NAP; 2-hydroxyfluorene, 2-FLU; and 9-hydroxyphenanthren, 9-PHE) and a marker of oxidative DNA damage (8-hydroxy- 2′- deoxyguanosine, 8-OHdG) were measured by high performance liquid chromatography. CYP1A1 methylation was measured by pyrosequencing. Finally, mediation analysis was performed to investigate whether CYP1A1 methylation mediated smoking and occupational PAH co-exposure effect on oxidative DNA damage. Results We observed significant associations of smoking and 1-OHP co-exposure with CYP1A1 hypomethylation (OR: 1.87, 95% CI: 1.01–3.47) and high 8-OHdG (OR: 2.13, 95% CI: 1.14–3.97). There was a significant relationship between CYP1A1 hypomethylation and high 8-OHdG (1st vs. 3rd tertile = 1.58, 95% CI: 1.01–2.47, P for trend = 0.046). In addition, mediation analysis suggested CYP1A1 hypomethylation could explain 13.6% of effect of high 8-OHdG related to smoking and 1-OHP co-exposure. Conclusions Our findings suggested that the co-exposure effect of smoking and occupational PAH could increase the risk of oxidative DNA damage by a mechanism partly involving CYP1A1 hypomethylation. Electronic supplementary material The online version of this article (10.1186/s12940-019-0508-0) contains supplementary material, which is available to authorized users.
- Published
- 2019
46. Mediation effect of AhR expression between polycyclic aromatic hydrocarbons exposure and oxidative DNA damage among Chinese occupational workers
- Author
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Yanli Liu, Jisheng Nie, Ye Fu, Jinzhu Zhao, Yingying Niu, Hongjie Zhang, Huitao Zhang, Jin Yang, and Aimin Yang
- Subjects
Adult ,Male ,China ,Mediation (statistics) ,medicine.medical_specialty ,Health, Toxicology and Mutagenesis ,Urinary system ,Naphthols ,010501 environmental sciences ,Toxicology ,01 natural sciences ,law.invention ,Oxidative dna damage ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Asian People ,Naphthalenesulfonates ,law ,Occupational Exposure ,Internal medicine ,medicine ,Humans ,Deoxyguanosine ,Polycyclic Aromatic Hydrocarbons ,Coke ,Chromatography, High Pressure Liquid ,Polymerase chain reaction ,Carcinogen ,0105 earth and related environmental sciences ,Aspartic Acid ,Fluorenes ,Pyrenes ,biology ,Chemistry ,General Medicine ,Aryl hydrocarbon receptor ,030210 environmental & occupational health ,Pollution ,Reverse transcriptase ,Oxidative Stress ,Endocrinology ,Receptors, Aryl Hydrocarbon ,8-Hydroxy-2'-Deoxyguanosine ,Carcinogens ,biology.protein ,Environmental Pollutants ,DNA Damage - Abstract
Polycyclic aromatic hydrocarbons (PAH) are well-known to be carcinogenic and the mechanisms that it contributes to oxidative DNA damage and aryl hydrocarbon receptor (AhR)-dependent induction are also well understood. However, little is known about the associations between PAH exposure, AhR expression, and oxidative DNA damage. We investigated their associations of AhR expression and oxidative DNA damage related to PAH exposure among 310 workers from a coke-oven plant in China. Urine biomarkers of PAH exposure (2-hydroxynaphthalene, 2-NAP; 2-hydroxyfluorene, 2-FLU; 9-hydroxyphenanthren, 9-PHE; and 1-hydroxypyrene, 1-OHP) and a marker of oxidative damage (8-hydroxy- 2′- deoxyguanosine, 8-OHdG) were measured by high performance liquid chromatography. AhR expression in venous blood was measured by reverse transcription -polymerase chain reaction. The results showed that increasing levels of urinary 1-OHP was positively associated with high 8-OHdG (OR (95% CI) was 4.01 (1.41–11.45) for 4th quartile, compared with 1st quartile, P for trend = 0.013). The similar associations were also found between urinary 1-OHP and high-AhR expressions (4th vs. 1st quartile = 3.50, 95% CI: 1.24–9.87, P for trend = 0.029). A significant association between AhR expression and high 8-OHdG was also found (4th vs. 1st quartile = 2.44, 95% CI: 1.05–5.70, P for trend = 0.027). In addition, mediation analysis showed the AhR expression could explain 35.9% of the association of oxidative DNA damage related to PAH exposure. Our findings implicated that the association between PAH exposure and oxidative DNA damage may be mediated by AhR expression among Chinese occupational workers.
- Published
- 2018
47. Population Pharmacokinetic Analysis of Mizoribine in Chinese Renal Transplant Recipients
- Author
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Qing-Ling Fu, Huitao Zhang, B. Ren, Long-shan Liu, Su-xiong Deng, Jiajia Jiang, Ji-guang Fei, C. Wang, Likun Chen, Jiang Qiu, Guanghao Chen, and J. Li
- Subjects
Adult ,Male ,medicine.medical_specialty ,Population ,030232 urology & nephrology ,Urology ,Renal function ,Absorption (skin) ,030204 cardiovascular system & hematology ,Models, Biological ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Pharmacokinetics ,Humans ,Medicine ,education ,Aged ,Distribution Volume ,Transplantation ,education.field_of_study ,Mizoribine ,business.industry ,Bayes Theorem ,Middle Aged ,Kidney Transplantation ,Transplant Recipients ,Confidence interval ,Female ,Surgery ,Ribonucleosides ,business ,Immunosuppressive Agents ,medicine.drug - Abstract
Background We calculated the population pharmacokinetics of mizoribine in adult Chinese patients and compared the parameters with those of Japanese patients to determine whether there are any ethnic differences in blood concentration transition between these 2 populations. Methods The blood concentrations of mizoribine in 21 Chinese patients who were administered mizoribine after renal transplantation were measured at 304 time points. The absorption lag time, absorption rate constant, apparent distribution volume, and oral clearance were thereafter calculated and compared with the respective Japanese references. Results The absorption lag time, absorption rate constant, and apparent distribution volume calculated in this study were, respectively, 0.353 hour, 0.856 hour−1, and 0.776 L/kg. The oral clearance was calculated as 2.18 times the creatinine clearance using creatinine clearance as a function. The absorption rate constant, apparent distribution volume, and oral clearance are determinants of the maximum blood concentration, trough, and area under the blood concentration time curve. The relative absorption rate constant, apparent distribution volume, and oral clearance were 0.9-, 0.9-, and 1.2-fold, respectively, in Chinese patients compared with those in Japanese patients. These values are within the confidence limit, suggesting that there is no significant PK difference between the 2 ethnic groups. Conclusions Results of this study showed no ethnic difference in blood mizoribine concentration transition between Chinese and Japanese patients. In addition, the population pharmacokinetic parameters obtained in this study are useful in determining the initial dosage or in the Bayesian analysis of mizoribine concentrations using scarce time points.
- Published
- 2018
48. Effect of Early Immunosuppression Therapy on De Novo Anti–Human-Leukocyte-Antigen Antibody After Kidney Transplantation
- Author
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Huitao Zhang, Gan Huang, Yuxin Zheng, Shuang Wang, Wuguo Deng, Qing-Ling Fu, Chun-Zhi Wang, Ronghai Deng, J. Li, Long-shan Liu, and Huiting Huang
- Subjects
Adult ,Graft Rejection ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,030232 urology & nephrology ,030230 surgery ,Gastroenterology ,Mycophenolic acid ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,HLA Antigens ,Internal medicine ,medicine ,Humans ,Transplantation, Homologous ,Dosing ,Prospective cohort study ,Kidney transplantation ,Autoantibodies ,Retrospective Studies ,Transplantation ,business.industry ,Graft Survival ,Hazard ratio ,Retrospective cohort study ,Immunosuppression ,Mycophenolic Acid ,medicine.disease ,Kidney Transplantation ,Calcineurin ,Female ,Surgery ,business ,Immunosuppressive Agents ,medicine.drug - Abstract
The aim of the study was to investigate the effect of immunosuppression therapy early after kidney transplantation, particularly exposure of mycophenolic acid (MPA) and calcineurin inhibitor (CNI), on posttransplantation de novo HLA antibody production. Methods A single-center retrospective cohort study was performed at the First Affiliated Hospital of Sun Yat-sen University, enrolling the kidney transplant or pancreas-kidney transplant recipients who had surgery between January 2010 and February 2016. Results A total of 214 recipients were included in the study with a median follow-up period of 1.06 years. A total of 30 recipients (14.0%) were positive in HLA antibody detection posttransplant with a median follow-up period of 1.46 years. Ten recipients (4.7%) lost their allograft function during follow-up, and 6 of them (60%) developed de novo HLA antibody after graft failure. Multivariate analysis showed that acute rejection significantly increased the risk of de novo HLA antibody (hazard ratio [HR], 2.732). Intensified MPA dosing therapy reduced the risk by 59.8% (HR, 0.402); low-dose CNI therapy increased the risk by 33.3% (HR, 1.333), and the effect of extremely low-dose CNI therapy was even larger (HR, 2.242). Conclusion The risk of de novo HLA antibody can be decreased by reducing the risk of acute rejection. A tendency was seen in low-dose CNI therapy to increase the risk of de novo HLA antibody, but intensified MPA dosing therapy may provide an umbrella protection effect by reducing the risk. Prospective study was required to confirm the effects.
- Published
- 2018
49. Silica/antibody-conjugated microcomb electrode sensor for cardiac biomarker analysis
- Author
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Thangavel Lakshmipriya, Huitao Zhang, Yuan Seng Wu, Haitao Li, Jing Li, Xingzhou Zhao, Shujiang Song, Subash C. B. Gopinath, Zihan Xue, and Jinpeng Xu
- Subjects
010302 applied physics ,Detection limit ,medicine.medical_specialty ,biology ,Chemistry ,02 engineering and technology ,General Chemistry ,Gold standard (test) ,Conjugated system ,021001 nanoscience & nanotechnology ,medicine.disease ,01 natural sciences ,Silica nanoparticles ,Internal medicine ,0103 physical sciences ,medicine ,Cardiology ,biology.protein ,Interdigitated electrode ,General Materials Science ,Biomarker Analysis ,Myocardial infarction ,Antibody ,0210 nano-technology - Abstract
Myocardial infarction (MI), which is generally known as a heart attack, occurs when there is a lack of blood supply to the heart. Electrocardiogram (ECG) is commonly used for the initial screening of MI but is not sufficiently sensitive. With ECG, cardiac biomarkers help to efficiently assess MI. The cardiac troponin I level has a high correlation with MI and is one of the gold standard biomarkers to identify MI. This study focuses on detecting cardiac troponin I by its parent antibody on a microcomb interdigitated electrode (IDE) sensor. To improve the detection, silica nanoparticles (SiNps) were complexed with antibody and immobilized on the IDE sensing surface. Amine-modified SiNps help to hold more antibodies on the sensing surface and interact with more cardiac troponin I molecules, leading to sensitive detection with a limit of detection of 1 fM (24 pg/mL). Moreover, the control proteins were failed to specifically interact, indicating the genuine detection of cardiac troponin I. This study helps to more easily and efficiently quantify the cardiac troponin I level to diagnose MI.
- Published
- 2021
50. A method for material decomposition and quantification with grating based phase CT
- Author
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Peiping Zhu, Qian Wang, Yining Zhu, Huitao Zhang, Shi-Wo Deng, Peng Zhang, and Kai Zhang
- Subjects
Computer science ,Contrast Media ,Optical Analysis ,Computed tomography ,01 natural sciences ,Diagnostic Radiology ,030218 nuclear medicine & medical imaging ,Synchrotron Radiation ,0302 clinical medicine ,Medicine and Health Sciences ,Signal Amplification ,Tomography ,Multidisciplinary ,medicine.diagnostic_test ,Refractive Index ,Phantoms, Imaging ,Radiology and Imaging ,Applied Mathematics ,Simulation and Modeling ,Physics ,Electromagnetic Radiation ,Phase-contrast imaging ,Bone Imaging ,Physical Sciences ,Engineering and Technology ,Medicine ,Algorithm ,Algorithms ,Research Article ,Imaging Techniques ,Iterative method ,Science ,Phase (waves) ,Neuroimaging ,Iterative reconstruction ,Research and Analysis Methods ,010309 optics ,03 medical and health sciences ,Diagnostic Medicine ,0103 physical sciences ,medicine ,Humans ,Computer Simulation ,Chemical Characterization ,Biology and Life Sciences ,Computed Axial Tomography ,X-Ray Radiography ,Noise ,Simultaneous Algebraic Reconstruction Technique ,Signal Processing ,Tomography, X-Ray Computed ,Mathematics ,Neuroscience - Abstract
Material decomposition (MD) is an important application of computer tomography (CT). For phase contrast imaging, conventional MD methods are categorized into two types with respect to different operation sequences, i.e., “before” or “after” image reconstruction. Both categories come down to two-step methods, which have the problem of noise amplification. In this study, we incorporate both phase and absorption (PA) information into MD process, and correspondingly develop a simultaneous algebraic reconstruction technique (SART). The proposed method is referred to as phase & absorption material decomposition-SART (PAMD-SART). By iteratively solving an optimization problem, material composition and substance quantification are reconstructed directly from absorption and differential phase projections. Comparing with two-step MD, the proposed one-step method is superior in noise suppression and accurate decomposition. Numerical simulations and synchrotron radiation based experiments show that PAMD-SART outperforms the classical MD method (image-based and dual-energy CT iterative method), especially for the quantitative accuracy of material equivalent atomic number.
- Published
- 2021
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