34 results on '"Liu, R. F."'
Search Results
2. Molecular adsorption and strain-induced ferromagnetic semiconductor-metal transition in half-hydrogenated germanene.
- Author
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Wang, X., Liu, G., Liu, R. F., Luo, W. W., Sun, B. Z., Lei, X. L., Ouyang, C. Y., and Xu, B.
- Subjects
TETRACYANOQUINODIMETHANE ,FERROMAGNETIC materials ,STRAINS & stresses (Mechanics) ,ELECTROPHILES ,CHARGE transfer ,DEFORMATIONS (Mechanics) ,FERMI level - Abstract
Very recently, half-hydrogenated germanene has been achieved in an experiment. In this paper, we investigate the effects of tetracyanoquinodimethane (TCNQ) molecular adsorption and strain on the electronic properties of half-hydrogenated germanene through first-principles. As an electron-acceptor molecule, TCNQ is exploited to non-covalently functionalize the half-hydrogenated germanene. However, this physical adsorption induces a ferromagnetic semiconductor–metal transition in half-hydrogenated germanene due to charge transfer from the substrate to the TCNQ molecule. More importantly, the superstructure of half-hydrogenated germanene/TCNQ is extremely sensitive to biaxial tensile strain. Under the biaxial tensile strain of 0.25%, the ferromagnetic semiconductor–metal transition induced by molecular adsorption can surprisingly be overturned. Meanwhile, a strong p-type doping is exhibited. Remarkably, it would return from a ferromagnetic semiconductor to a metal again when the biaxial tensile strain increases to 1.5%. Our analysis based on the structural and electronic properties of half-hydrogenated germanene/TCNQ indicates that such metal–semiconductor–metal transition in half-hydrogenated germanene/TCNQ under biaxial tensile strain may originate from the strong local deformation, resulting in the energy of the valence band maximum decreasing below or increasing above the Fermi level. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
3. Hydrothermal-electrochemical codeposited hydoxyapatite/yttria-stabilized zirconia composite coating
- Author
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Xiao, X. F., Liu, R. F., and Zheng, Y. Z.
- Published
- 2006
- Full Text
- View/download PDF
4. The Application of K Value Adaptive VMD Method in Pipeline Leakage Location.
- Author
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Liu, R. F., Yang, M. J., Sun, C. Q., and Zeng, S.
- Subjects
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SIGNAL separation , *LEAKAGE , *DECOMPOSITION method , *WATER distribution , *WATER supply - Abstract
The research about online monitoring and leakage automatic location of water distribution networks (WDN) has a wide range of applications that include water resource protection, monitoring, and allocation. Variational mode decomposition (VMD) and cross-correlation (CC) based leakage location is a popular and effective method in WDN. However, the value of K intrinsic mode functions (IMFs) based on VMD decomposition needs to be determined artificially, which affects the separation effect of signal frequency band characteristics directly. Hence, this work proposes an adaptive method to determine the parameter K of leakage vibration signal's IMFs, which will be applied to automatic leakage location in WDN. Firstly, the number of saddle points in the frequency domain envelope of the sampled signal in different step sizes is calculated. The parameter K is determined according to the curvature change of the number of saddle points and the sampled signal. Finally, the selective IMFs are reconstituted into a new signal, which can determine a leak position using CC based time-delay estimation (TDE). To verify the effectiveness of the proposed algorithm, the different methods based on EMD and Fast ICA are compared. The experimental results demonstrate that the proposed parameter K value adaptive VMD (KVA-VMD) decomposition method is more suitable for leakage location in WDN. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
5. FLGC-Fusion GAN: An Enhanced Fusion GAN Model by Importing Fully Learnable Group Convolution.
- Author
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Yuan, C., Sun, C. Q., Tang, X. Y., and Liu, R. F.
- Subjects
IMAGE fusion ,MATHEMATICAL convolutions ,INFRARED radiation ,NETWORK effect - Abstract
The purpose of image fusion is to combine the source images of the same scene into a single composite image with more useful information and better visual effects. Fusion GAN has made a breakthrough in this field by proposing to use the generative adversarial network to fuse images. In some cases, considering retain infrared radiation information and gradient information at the same time, the existing fusion methods ignore the image contrast and other elements. To this end, we propose a new end-to-end network structure based on generative adversarial networks (GANs), termed as FLGC-Fusion GAN. In the generator, using the learnable grouping convolution can improve the efficiency of the model and save computing resources. Therefore, we can have a better trade-off between the accuracy and speed of the model. Besides, we take the residual dense block as the basic network building unit and use the perception characteristics of the inactive as content loss characteristics of input, achieving the effect of deep network supervision. Experimental results on two public datasets show that the proposed method performs well in subjective visual performance and objective criteria and has obvious advantages over other current typical methods. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
6. Rod-like CuMnOx transformed from mixed oxide particles by alkaline hydrothermal treatment as a novel catalyst for catalytic combustion of toluene.
- Author
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Li, W. B., Liu, Z. X., Liu, R. F., Chen, J. L., and Xu, B. Q.
- Abstract
Rod-like copper manganese mixed oxides (CuMn
x -NR) have been synthesized from copper manganese mixed oxide particles by sodium hydroxide hydrothermal treatment, and a higher BET surface area of 221 m2 g−1 is obtained on the nanorod-like sample, which exhibits superior catalytic activity toward toluene combustion at 210 °C due to the increase in its oxygen mobility of the chemisorbed oxygen species as well as the increase in surface concentrations of higher valance cations, Cu2+ , Mn3+ and Mn4+ , in the samples. [ABSTRACT FROM AUTHOR]- Published
- 2016
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- View/download PDF
7. Expression and activity analysis reveal that heme oxygenase (decycling) I is associated with blue egg formation.
- Author
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Wang, Z. P., Liu, R. F., Wang, A. R., Li, J. Y., and Deng, X. M.
- Subjects
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HEME oxygenase , *EGGS , *GENE expression , *EGGSHELLS , *MESSENGER RNA - Abstract
Biliverdin is responsible for the coloration of blue eggs and is secreted onto the eggshell by the shell gland. Previous studies confirmed that a significant difference exists in biliverdin content between blue eggs and brown eggs, although the reasons are still unknown. Because the pigment is derived from oxidative degradation of heme catalyzed by heme oxygenase (HO), this study compared heme oxygenase (decycling) 1 (HMOX1), the gene encoding HO expression and HO activity, in the shell glands of the Dongxiang blueshelled chicken (n = 12) and the Dongxiang brownshelled chicken (n = 12). Results showed that HMOX1 was highly expressed at the mRNA (1.58-fold; P < 0.05) and protein levels in blue-shelled chickens compared with brown-shelled chickens. At the functional level, blue-shelled chickens also showed 1.40-fold (P < 0.05) higher HO activity than brown-shelled chickens. To explore the reasons for the differential expression of HMOX1, an association study of 6 SNP capturing the majority of HMOX1 variants with the blue egg coloration was performed. Results showed no significant association between SNP and the blue egg coloration in HMOX1 (P > 0.05). Taken together, these results show that blue egg formation is associated with high expression of HMOX1 in the shell gland of Dongxiang blue-shelled chickens, and suggest that differential expression of HMOX1 in the 2 groups of chickens is most likely to arise from an alteration in the trans-acting factor. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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8. The biomimetic deposition of carbonate apatite induced by titanium base calcium enriched silica films.
- Author
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Xiao, X. F., Liu, R. F., and Gao, Y. J.
- Subjects
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BIOMIMETIC chemicals , *CARBONATES , *APATITE , *TITANIUM , *CALCIUM silicates - Abstract
In order to obtain a fast biomimetic coating on titanium substrates, calcium enriched silica films were prepared on pure titanium substrates by sol-gel method under the following conditions: pH = 3 and the molar ratio of tetraethyl orthosilicate (TEOS)/H2O/EtOH/Ca2+ = 1∶4∶11∶0·08. The titanium base calcium enriched silica film is soaked in a slightly supersaturated Ca/P solution, with the concentration of calcium ion and phosphate group being 1·5 times of that of simulated body fluid, for seven days, a uniform apatite layer is precipitated onto the surfaces of calcium enriched silica films, which is quite rapidly. The investigation adopted SEM, EDX, Fourier transform infrared spectroscope and XRD to indicate that the coating was carbonate hydroxyapatite. The results show that it is a feasible way to obtain carbonate hydroxyapatite through functionalising the pure titanium substrates by preparing calcium enriched silica films on it from a sol-gel method. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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9. Preparation and bioactivity of embedded-style hydroxyapatite--titania nanotube arrays.
- Author
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XIAO, X.-F., LIU, R.-F., and TIAN, T.
- Subjects
- *
HYDROXYAPATITE , *NANOTUBES , *TITANIUM compounds , *ELECTROLYTIC oxidation , *MICROSTRUCTURE , *CRYSTALLIZATION - Abstract
Embedded-style hydroxyapatite-titania nanotube arrays were successfully prepared by anodic oxidation of titanium substrate and centrifugal filling hydroxyapatite precursor sol into hollow nanotubes. The morphology, microstructure and thermal stability of the samples were characterized by X-ray diffraction, environmental scanning electron microscopy, and energy dispersive X-ray analysis. The results show that the structure of titania nanotube arrays is stable at 500 °C or below, and the crystallized hydroxyapatite could be formed from hydroxyapatite precursor sol after calcining at 500 °C for 4 h. The optimum calcining temperature for this material is 500 °C. An obvious apatite layer formed on the surface of the embedded-style material after soaking in simulated body fluid for 5 days, indicating that the material possesses a good in vitro apatite forming ability on its surface. [ABSTRACT FROM AUTHOR]
- Published
- 2009
10. Hydrothermal preparation of nanocarbonated hydroxyapatite crystallites.
- Author
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Xiao, X.-F., Liu, R.-F., and Gao, Y.-J.
- Subjects
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HYDROXYAPATITE , *CALCIUM , *UREA , *CRYSTALLIZATION , *CARBON , *MICROSTRUCTURE - Abstract
Needle-like nanocarbonated hydroxyapatite (CHA) powders were prepared by hydrothermal method using Ca(NO3)2, (NH4)3PO4 and urea as reagents. Element analyse (EMA), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and thermogravimetry differential thermogravimetry (TG-DTA) were used to characterise the crystalline phase, microstructure, chemical composition, morphology and thermal stability of CHA. The results show that AB type CHA can be obtained through this method. The carbonate content and the ratio of length to diameter of CHA can be adjusted by controlling the concentration of urea. The results show that CO32-incorporated into the apatite structure has an influence on the properties of CHA, such as the morphology and the size of crystal grain, the lattice parameters and the unit cell volume and the crystallisation degree and the stability. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
11. Biomimetic synthesis of spherical nano-hydroxyapatite with polyvinylpyrrolidone as template.
- Author
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Qiu, C. F., Xiao, X. F., Liu, R. F., and She, H. D.
- Subjects
HYDROXYAPATITE ,MATERIALS analysis ,CHEMICAL reagents ,POVIDONE ,X-ray spectroscopy ,TRANSMISSION electron microscopy - Abstract
Spherical nano-hydroxyapatite (HA) was synthesised successfully. Ca(NO
3 )2 4H2 O and (NH4 )3 PO4 3H2 O were used as reagents and polyvinylpyrrolidone (PVP) as template. The crystal phase, chemical composition, microstructure of the obtained HA were characterised by X-ray powder diffraction analysis, Fourier transform infrared spectroscopy, X-ray fluorescence spectrometry, thermogravimetric analysers microcomputer differential thermal analysers and transmission electron microscopy. The results show that the concentration of PVP significantly affects the morphologies of the nanometre HA. Spherical nano-hydroxyapatite particle with diameter of 30–50 nm can be synthesised using PVP of a certain concentration (05–5%) as template. The crystallinity of HA decreases continuously as the PVP content increases. Spherical apatite crystal forms because of a high affinity between HA and PVP. [ABSTRACT FROM AUTHOR]- Published
- 2008
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- View/download PDF
12. Hydroxyapatite nanospindles by biomimetic synthesis with chitosan as template.
- Author
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He, D., Xiao, X. F., Liu, F., and Liu, R. F.
- Subjects
CHITOSAN ,CHITIN ,MICROSTRUCTURE ,MATERIALS ,FOURIER transforms ,INFRARED spectroscopy - Abstract
Based on the principles of biomineralisation, spindle shaped hydroxyapatites (HA) were synthesised through the biomimetic method with chitosan (CS) as template. The microstructure, chemical composition and thermal behaviour were characterised by means of X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy and thermogravimetry differential thermal analysis (TGDTA) respectively. The results show that the spindle shaped nanoHA, 30–40 nm in length and 7–8 nm in width, were synthesised with 0·5 wt-%CS as template in 6% (v/v) hydrogen peroxide solution for 7 days. The crystallinity of samples increased with the aging time. TGDTA analyses show that the HA powders synthesised with CS as template contain CS molecules and have good thermal stability up to 800°C. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
13. Image Gallery: Trigeminal trophic syndrome: nonhealing facial ulcer with corneal ulceration.
- Author
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Wu, M.‐Y., Liu, R.‐F., and Chang, Y.‐Y.
- Subjects
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ULCERS , *SYNDROMES , *MEDULLA oblongata , *THERAPEUTICS , *CRANIAL nerves - Published
- 2019
- Full Text
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14. Yield and nutritive value of rapeseed protein concentrate
- Author
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Jones, J. D., Liu, R. F. K., and Thompson, L. U.
- Subjects
- *
RAPESEED , *PROTEINS - Published
- 1982
15. Functional properties and food applications of rapeseed protein concentrate
- Author
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Jones, J. D., Thompson, L. U., and Liu, R. F. K.
- Subjects
FOOD industry ,NUTRITION ,PROTEINS ,RAPESEED - Published
- 1982
16. ON THE FALSE DEGENERACY OF THE HELMHOLTZ BOUNDARY INTEGRAL EQUATIONS
- Author
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YEIH, W., KUO, S. R., LIU, R. F., and CHANG, J. R.
- Subjects
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INTEGRAL equations , *HELMHOLTZ equation , *BOUNDARY value problems , *EIGENVALUES - Abstract
In this paper, the false degeneracy of the Helmholtz boundary integral equations is examined. A new theory to explain the false degeneracy of the Helmholtz boundary integral equations is given. In this proposed theory, a false degeneracy of the boundary integral equation is explained as finding a non-trivial source distribution such that it results in trivial field quantities inside the domain interested and non-trivial field quantities for its counter part, i.e., outside the domain interested. It is clearly explained that such a false degeneracy is independent of prescribed boundary conditions but dependent on the integral equation one selects. Moreover, the false degeneracy of the integral equation for the interested domain relates to the eigenproblem for its counter part. Under such a unified theory, the fictitious eigenvalue, spurious eigenvalue and pseudo-fictitious eigenvalue can be explained in a simple mathematical frame. It is concluded from our theoretical analysis that a multiply connected domain results in the pseudo-fictitious eigenvalue even the complex-valued formulations are used. In order to eliminate various kinds of false degeneracy, two methods are employed according to the previous research. A unified view of these two methods is provided such that they can be thought to be equivalent from mathematical point of view. Several numerical examples are given to show the validity of current approach. [Copyright &y& Elsevier]
- Published
- 2002
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17. Effects of lysine deficiency or excess on growth and the expression of lipid metabolism genes in slow-growing broilers.
- Author
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Tian, D L, Guo, R J, Li, Y M, Chen, P P, Zi, B B, Wang, J J, Liu, R F, Min, Y N, Wang, Z P, Niu, Z Y, and Liu, F Z
- Subjects
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CHOLESTERYL ester transfer protein , *LIPID metabolism , *LIPID synthesis , *ACETYL-CoA carboxylase , *ABDOMINAL adipose tissue , *CARRIER proteins - Abstract
This experiment was conducted to evaluate the effects of lysine deficiency or excess on growth and the expression of lipid metabolism genes in slow-growing birds. A total of 360 one-day-old chicks were randomly divided into 3 groups, with 6 replicates of 20 birds each. The birds fed the basal diet with a total lysine 0.60% (LL), 1.00% (ML), or 1.40% (HL). The amount of lysine (ML) as the control group, LL and HL as the experimental group, the trial period last 3 wk. The results showed that compared with ML, LL significantly decreased average daily gain and average daily feed intake and remarkably increased feed conversion ratio of birds at 21 day old (P < 0.01), while the above indices in HL had no significant effects (P > 0.05). Besides, LL reduced the pectoral muscle rate (P < 0.01) and decreased the percentage of abdominal fat significantly (P < 0.05). In addition, compared with ML, the expression of fatty acid binding protein 1 (FABP1), acetyl-CoA carboxylase (ACC), malic enzyme (ME), and sterol regulatory element binding protein 1 (SREBP1c) mRNA of liver in LL was significantly decreased (P < 0.05), and the expression of cholesteryl ester transfer protein (CETP) mRNA was significantly increased (P < 0.01), whereas LL had no significant effects on the expression of peroxisome proliferator activated receptor alpha (PPARα) mRNA (P > 0.05). Moreover, compared with ML, HL significantly reduced the expression of FABP1, ACC, ME, SREBP-1c , and PPARα mRNA in the liver (P < 0.05), and had no significant effects on the expression of CETP mRNA (P > 0.05). The results of current research suggest that dietary lysine deficiency could reduce the growth and fat deposition of slow-growing broilers mainly by downregulating the expression of lipid synthesis genes. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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18. [A single-center study on the oncological outcomes of active surveillance of thyroid nodules measuring≤1 cm with highly suspicious ultrasound features].
- Author
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Liu CH, Zhao H, Xia Y, Cao Y, Zhang LY, Zhao Y, Gao LY, Liu RF, Liu YW, Liu HF, Meng ZL, Liu SZ, Lu Y, Palashate Y, and Li XY
- Subjects
- Male, Female, Humans, Adult, Middle Aged, Cohort Studies, Prospective Studies, Watchful Waiting, Ultrasonography methods, Retrospective Studies, Disease Progression, Thyroid Nodule diagnostic imaging, Thyroid Nodule pathology, Thyroid Neoplasms
- Abstract
Objective: To investigate the oncological outcomes of active surveillance (AS) in patients showing thyroid nodules measuring≤1 cm with highly suspicious ultrasound features. Methods: A prospective single-center cohort study. A total of 534 patients with highly suspicious thyroid nodules (2015 American Thyroid Association Nodule Sonographic Patterns and Risk of Malignancy: High Suspicion) were enrolled in this study, the patients received AS at Peking Union Medical College Hospital between January 2017 and November 2022 to assess oncological outcomes (disease progression, recurrence/metastasis rate, etc). The patients were followed up every 6 months for physical examination and neck ultrasound examination. And the value of tumor volume changes in evaluating tumor enlargement was explored too. Results: There were 413 females and 121 males in this cohort, with a mean age of (42.6±11.8) years. During a median follow-up period of 45.6 months (ranged from 3.5 to 176.0 months), disease progression occurred in 26 patients (4.9%) with highly suspicious thyroid nodules, characterized by a minimum 3-mm increase in tumor diameter in 19 patients (3.6%) and lymph node metastases in 7 patients (1.3%). Forty-seven (8.8%) patients opted for delayed surgery, with 29 patients due to a change in preference. There was no significant differences in pathologic and follow-up outcomes between patients with disease progression and preference change. Patients aged≤40 years had a higher cumulative incidence of 5-year disease progression than those aged>40 years (4.9% vs 1.9%, P =0.060). No patients experienced distant metastases or deaths. Among the 595 high-risk thyroid nodules with continuous volume assessment results and an increase in nodule diameter of less than 3 mm (including all high-risk nodules in patients with single or multiple nodules), 184 (30.9%) and 79 (13.3%) nodules exhibited volume increases of more than 50% and 100%, respectively, in multiple measurements. Among the nodules with volume changes exceeding 50% and 100%, the proportion of nodules with a baseline tumor diameter of≤0.5 cm was significantly higher than those with a diameter of>0.5 cm, at 69.0% vs 31.0% ( P <0.001) and 77.2% vs 22.8% ( P <0.001), respectively . Conclusions: Active surveillance in patients with highly suspicious subcentimeter thyroid nodules has good short-term oncological outcomes and can be considered a safe alternative to surgery. Due to the large variability in the measurement results of tumor volume, it is not suitable as an indicator for evaluating tumor enlargement .
- Published
- 2023
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- View/download PDF
19. Strain-tunable molecular doping in germanane: a first-principles study.
- Author
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Wang X, Liu G, Liu RF, Luo WW, Wu MS, Sun BZ, Lei XL, Ouyang CY, and Xu B
- Abstract
Germanane, fully hydrogenated germanene, has recently attracted great interest, both theoretical and experimental. In this paper we thoroughly study strain-tunable n/p-type doping in germanane by adsorption of tetrathiafulvalene (TTF)/tetracyanoquinodimethane (TCNQ) molecules through first-principles calculations. The results show that both TTF and TCNQ molecules can non-covalently functionalize the electronic properties of germanane. Not surprisingly, TTF molecular adsorption induces n-type doping in germanane because the TTF molecule is a typical electron donor. Moreover, a linearly tunable band gap of germanane and differing n-type doping strengths can be realized by a biaxial strain ranging from -3% to 3%. Analysis indicates that tensile strain would promote the doping effect whereas compressive strain would inhibit it. Comparatively, TCNQ molecular adsorption induces a germanane/TCNQ system which exhibits metallic characteristics. Surprisingly, however, under a tensile strain of 2.5%, a strong p-type doping effect is achieved in germanene/TCNQ. In particular, with increasing tensile strain over the range 2.5%-3%, the strain-tunable p-type doping effect decreases gradually. Such a multiple effect of molecular adsorption and strain on the electronic properties of germanane could be helpful for potential future applications of germanane-based electron devices.
- Published
- 2018
- Full Text
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20. [Clinical research about needle-tract assisted standard percutaneous nephrolithotomy for the treatment of complicated upper urinary tract calculi].
- Author
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Duan B, Chen B, Huang HC, Liu RF, Wang HQ, Zheng JX, Zeng YK, and Xing JC
- Subjects
- Humans, Retrospective Studies, Treatment Outcome, Urinary Calculi, Kidney Calculi therapy, Nephrolithotomy, Percutaneous
- Abstract
Objective: To investigate the safety and efficacy of needle-tract assisted standard percutaneous nephrolithotomy (PCNL) for the treatment of complicated upper urinary tract calculi. Methods: The clinical data of 1 562 patients with complicated upper urinary calculi who received standard PCNL from December 2013 to December 2017 at Department of Urology, the First Affiliated Hospital of Xiamen University were retrospectively analyzed. There were large residual stones in 256 patients through B-ultrasound exploration after standard PCNL, could't be detected with nephoscope in standard PCNL tracts. 16 F mini PCNL tract were established in 120 cases for treatment of residual stones, while needle-tract were established in order to guide nephroscope to find residual stones in 126 cases. Needle-tract were transferred to 16 F mini PCNL tract for treatment of residual stones in 10 patients if these residual stones could't be detected through needle-tract. Operation time, change of hemoglobin level after operation, incidence of postoperative complications, time of hospitalization and rate of stone clearance were measured in two groups. The statistical methods used included t test, Wilcoxon rank sum test, and χ
2 test. Results: There were 1 to 3 mini tracts ( M ( QR ): 1(1)) established in the mini tracts group and 1 to 7 needle-tracts ( M ( QR ): 3(2)) established in the needle-tract group ( Z =-10.57, P =0.000). Compared with mini tract group, the operation time ((62.0±18.0) minutes vs . (84.0±15.5) minutes, t =10.242, P =0.000), hospitalization time ((4.40±0.86) days vs . (5.20±0.81) days, t =7.570, P =0.049), hemoglobin dropped ((1.31±0.47) g/L vs . (2.74±0.63) g/L, t =20.12, P =0.000), and incidence of postoperative complications (7.9% (10/126) vs . 19.2% (23/120), χ2 =6.674, P =0.01) of needle-tract group were lower, while postoperative stone clearance rate was higher (89.7% vs . 76.7%, χ2 =7.497, P =0.006). No perioperative severe complications such as pleural injury, pneumatothorax, perforation of renal, trauma of abdominal organ occurred in two groups. Conclusion: Needle-tract assisted standard PCNL for the treatment of complicated upper urinary calculi can significantly improve stone clearance rate, reduce operation time, decrease risk of kidney and surrounding organs damage.- Published
- 2018
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21. Increased miR-155-5p expression in dermal mesenchymal stem cells of psoriatic patients: comparing the microRNA expression profile by microarray.
- Author
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Hou RX, Liu RF, Zhao XC, Jia YR, An P, Hao ZP, Li JQ, Li XH, Yin GH, and Zhang KM
- Subjects
- Adaptor Proteins, Signal Transducing metabolism, Adult, Case-Control Studies, Dermis pathology, Female, Humans, Male, Mesenchymal Stem Cells pathology, MicroRNAs metabolism, Middle Aged, Nitric Oxide Synthase Type II genetics, Nitric Oxide Synthase Type II metabolism, Oligonucleotide Array Sequence Analysis, Psoriasis metabolism, Psoriasis pathology, Real-Time Polymerase Chain Reaction, Severity of Illness Index, Signal Transduction, Adaptor Proteins, Signal Transducing genetics, Dermis metabolism, Gene Expression Regulation, Mesenchymal Stem Cells metabolism, MicroRNAs genetics, Psoriasis genetics
- Abstract
Mesenchymal stem cells (MSCs) have pleiotropic immuno-modulatory effects and pro-angiogenic ability, leading to the presumption that MSCs may be involved in the pathogenesis of many inflammatory or autoimmune disorders, including psoriasis. In a previous study, we reported the specific gene expression profile of dermal MSCs from psoriasis. Inflammation- and angiogenesis-related genes, such as lipopolysaccharide-induced tumor necrosis factor-alpha transcription factor (LITAF), dual-specificity protein phosphatase 1 (DUSP1), vascular endothelial growth factor α (VEGFα), and insulin-like growth factor-binding protein-5 (IGFBP5), are abnormally expressed in psoriatic dermal MSCs. As a key regulator of gene expression, miRNA are involved in a wide variety of biological processes; in fact, several miRNAs have been implicated in the development and progression of inflammatory or autoimmune disorders. In this study, we compared the miRNA expression profiles of dermal MSCs from patients with psoriasis to those in MSCs from normal individuals by microarray, and found that the pro-inflammatory miRNA miR-155 was significantly overexpressed in psoriatic MSCs (2.44 fold, P < 0.001). Additionally, the expression of miR-155 target gene TAB2 (8.47 ± 1.55 vs 6.38 ± 2.10, P < 0.01,) and the downstream gene iNOS (5.26 ± 2.58 vs 3.73 ± 1.89, P < 0.05) was found to be inhibited in psoriatic dermal MSCs by real-time PCR. Therefore, we speculated that the elevation in miR-155 levels may be an indicator of, or a key regulatory pathway in, the pathogenesis of psoriasis, resulting in functionally impaired dermal MSCs.
- Published
- 2016
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22. Rod-like CuMnOx transformed from mixed oxide particles by alkaline hydrothermal treatment as a novel catalyst for catalytic combustion of toluene.
- Author
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Li WB, Liu ZX, Liu RF, Chen JL, and Xu BQ
- Abstract
Rod-like copper manganese mixed oxides (CuMnx-NR) have been synthesized from copper manganese mixed oxide particles by sodium hydroxide hydrothermal treatment, and a higher BET surface area of 221 m(2) g(-1) is obtained on the nanorod-like sample, which exhibits superior catalytic activity toward toluene combustion at 210 °C due to the increase in its oxygen mobility of the chemisorbed oxygen species as well as the increase in surface concentrations of higher valance cations, Cu(2+), Mn(3+) and Mn(4+), in the samples.
- Published
- 2016
- Full Text
- View/download PDF
23. Research Note Mesenchymal stem cells from skin lesions of psoriasis patients promote proliferation and inhibit apoptosis of HaCaT cells.
- Author
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Liu RF, Wang F, Wang Q, Zhao XC, and Zhang KM
- Subjects
- Adult, Antigens, CD biosynthesis, Antigens, CD genetics, Apoptosis genetics, Cell Differentiation genetics, Cell Proliferation genetics, Epidermis metabolism, Epidermis pathology, Female, Flow Cytometry, Humans, Keratinocytes metabolism, Male, Mesenchymal Stem Cells metabolism, Middle Aged, Psoriasis pathology, Skin metabolism, Keratinocytes pathology, Mesenchymal Stem Cells pathology, Psoriasis genetics, Skin pathology
- Abstract
Psoriasis is an inflammatory skin disease characterized by excessive proliferation and abnormal differentiation and apoptosis of keratinocytes (KCs). Mesenchymal stem cells (MSCs) from skin lesions of psoriasis patients demonstrate abnormal cytokine secretion, which may affect KC proliferation and apoptosis. Here, we explored how MSCs from skin lesions of psoriasis patients affect HaCaT cell proliferation and apoptosis. First, flow cytometry and multipotent differentiation methods were used to identify skin MSCs, which were then co-cultured with HaCaT cells. HaCaT cell proliferation was analyzed in real-time, and cell cycle progression and apoptosis were assessed by flow cytometry. Cell morphologies and multipotencies of skin MSCs were similar between the psoriasis group and healthy control group, with high levels of CD29, CD44, CD73, CD90, and CD105 and limited expression of CD34, CD45, and HLA-DR. MSCs from skin lesions of psoriasis patients promote KC proliferation more potently and are less capable of inducing KC apoptosis. This may underlie KC proliferation and abnormal apoptosis in psoriasis skin lesions, which results in abnormal thickening of the epidermis.
- Published
- 2015
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24. LITAF, HHEX, and DUSP1 expression in mesenchymal stem cells from patients with psoriasis.
- Author
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Chang WJ, Niu XP, Hou RX, Li JQ, Liu RF, Wang Q, Wang CF, Li XH, Yin GH, and Zhang KM
- Subjects
- Adult, Case-Control Studies, Dual Specificity Phosphatase 1 metabolism, Female, Homeodomain Proteins metabolism, Humans, Male, Middle Aged, Nuclear Proteins metabolism, Psoriasis diagnosis, RNA, Messenger genetics, RNA, Messenger metabolism, Severity of Illness Index, Transcription Factors metabolism, Young Adult, Dual Specificity Phosphatase 1 genetics, Gene Expression, Homeodomain Proteins genetics, Mesenchymal Stem Cells metabolism, Nuclear Proteins genetics, Psoriasis genetics, Transcription Factors genetics
- Abstract
Psoriasis is a common chronic relapsing inflammatory skin disease, in which mesenchymal stem cells (MSCs) have been hypothesized to play an important role in abnormal localized inflammation and vascular proliferation observed in skin lesions. Previous studies have revealed abnormal gene expression patterns, DNA methylation status, and cytokine secretion of MSCs in psoriatic skin lesions, as well as some gene expression abnormalities related to inflammation and angiogenesis. We further verified the gene and protein expressions of inflammation-related lipopolysaccharide-induced tumor necrosis factor-alpha transcription factor (LITAF), dual-specificity protein phosphatase 1 (DUSP1), and angiogenesis-related hematopoietically expressed homeobox (HHEX) in MSCs derived from the skin lesions of psoriasis patients. The gene expression of LITAF, DUSP1, and HHEX in dermal MSCs was measured at the mRNA level using reverse transcription-polymerase chain reaction and the corresponding protein expression levels were analyzed by western blotting analysis. The gene and protein expression levels of LITAF, HHEX, and DUSP1 in dermal MSCs were significantly lower in psoriasis patients compared to controls. Amplification and western blotting results were consistent with our previously reported gene chip data. Our results suggest that dermal MSCs in psoriatic skin lesions may be involved in the development, progression, and regulation of localized inflammatory abnormalities by reducing the expression of LITAF, HHEX, and DUSP1, which are related to inflammation and angiogenesis.
- Published
- 2015
- Full Text
- View/download PDF
25. PRESBYOPIA OPTOMETRY METHOD BASED ON DIOPTER REGULATION AND CHARGE COUPLE DEVICE IMAGING TECHNOLOGY.
- Author
-
Zhao Q, Wu XX, Zhou J, Wang X, Liu RF, and Gao J
- Subjects
- Humans, Diagnostic Imaging instrumentation, Diagnostic Imaging methods, Eye pathology, Eye physiopathology, Microcomputers, Optometry instrumentation, Optometry methods, Presbyopia diagnosis, Presbyopia physiopathology
- Abstract
With the development of photoelectric technology and single-chip microcomputer technology, objective optometry, also known as automatic optometry, is becoming precise. This paper proposed a presbyopia optometry method based on diopter regulation and Charge Couple Device (CCD) imaging technology and, in the meantime, designed a light path that could measure the system. This method projects a test figure to the eye ground and then the reflected image from the eye ground is detected by CCD. The image is then automatically identified by computer and the far point and near point diopters are determined to calculate lens parameter. This is a fully automatic objective optometry method which eliminates subjective factors of the tested subject. Furthermore, it can acquire the lens parameter of presbyopia accurately and quickly and can be used to measure the lens parameter of hyperopia, myopia and astigmatism.
- Published
- 2015
26. Salt stress represses production of extracellular proteases in Bacillus pumilus.
- Author
-
Liu RF, Huang CL, and Feng H
- Subjects
- Bacillus drug effects, Endopeptidases genetics, Fermentation, Gene Expression Regulation, Bacterial drug effects, Sodium Chloride pharmacology, Bacillus enzymology, Endopeptidases biosynthesis, Stress, Physiological drug effects
- Abstract
Bacillus pumilus is able to secrete subtilisin-like prote-ases, one of which has been purified and characterized biochemically, demonstrating great potential for use in industrial applications. In the current study, the biosynthesis and transcription of extracellular pro-teases in B. pumilus (BA06) under salt stress were investigated using various methods, including a proteolytic assay, zymogram analysis, and real-time PCR. Our results showed that total extracellular proteolytic activity, both in fermentation broth and on milk-containing agar plates, was considerably repressed by salt in a dosage-dependent manner. As Bacillus species usually secret multiple extracellular proteases, a vari-ety of individual extracellular protease encoding genes were selected for real-time PCR analysis. It was shown that proteases encoded by the aprE and aprX genes were the major proteases in the fermentation broth in terms of their transcripts in B. pumilus. Further, transcription of aprE, aprX, and epr genes was indeed repressed by salt stress. In con-trast, transcription of other genes (e.g., vpr and wprA) was not repressed or significantly affected by the salt. Conclusively, salt stress represses total extracellular proteolytic activity in B. pumilus, which can largely be ascribed to suppression of the major protease-encoding genes (aprE, aprX) at the transcriptional level. In contrast, transcription of other pro-tease-encoding genes (e.g., vpr, wprA) was not repressed by salt stress.
- Published
- 2015
- Full Text
- View/download PDF
27. Impact of BMMSCs from different sources on proliferation of CD34⁺ cells.
- Author
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Liu RF, Li JQ, Hou RX, Wang R, and Zhang KM
- Subjects
- Adult, Cell Proliferation, Cell Separation, Cells, Cultured, Female, Flow Cytometry, Humans, Male, Middle Aged, Psoriasis pathology, Young Adult, Antigens, CD34 metabolism, Bone Marrow Cells cytology, Mesenchymal Stem Cells cytology
- Abstract
There are significant differences on the biological characteristics of bone marrow mesenchymal stem cells (BMMSCs), immunological response, and antigen-presenting functions between patients with psoriasis and normal subjects, but there are no significant differences in aborted fetuses. We examined the differences in BMMSCs between aborted fetuses and patients with psoriasis in this study. Bone marrow from normal subjects, aborted fetuses, and patients with psoriasis were obtained using a MidiMACS machine. Density gradient centrifugation method was used to isolate the bone marrow mononuclear cells of patients with psoriasis and aborted fetus and the cells were cultivated. Bone marrow CD34(+) cells from normal subjects were isolated. MTT colorimetric detection was used to test the proliferation activity of bone marrow CD34(+) cells. The purity of bone marrow CD34(+) cells and BMMSCs was determined by flow cytometry. The BMMSC culture supernatant fluid of patients with psoriasis and aborted fetuses showed no statistically significant difference with bone marrow CD34(+) cell proliferation in normal subjects (P > 0.05).
- Published
- 2015
- Full Text
- View/download PDF
28. [Pinworm infection in primary school students in Luoyang City].
- Author
-
Wang XC and Liu RF
- Subjects
- Child, China epidemiology, Female, Humans, Male, Prevalence, Enterobiasis epidemiology
- Published
- 2001
29. [Survey of pediculosis capitis in primary school pupils in the suburbs of Luoyang City].
- Author
-
Wang XC and Liu RF
- Subjects
- Child, Female, Humans, Male, Prevalence, Urban Health, Lice Infestations epidemiology
- Published
- 2000
30. [Prevention and treatment of intrauterine device caused abnormal menstruation by huan-an powder].
- Author
-
Liu RF
- Subjects
- Adult, Female, Hemoglobins metabolism, Humans, Menorrhagia drug therapy, Menorrhagia etiology, Menstruation Disturbances etiology, Menstruation Disturbances prevention & control, Drugs, Chinese Herbal therapeutic use, Intrauterine Devices adverse effects, Menstruation Disturbances drug therapy
- Abstract
Huan-an Powder (HA) was used to prevent and treat the intrauterine device (IUD) caused abnormal menstruation in 155 cases, who were divided into the prevention group and treatment group. In the former, 70 cases had taken the powder, resulting in no obvious difference in their amounts of menstrual blood, menstrual period, menstrual cycle and hemoglobin between after and before the applying of IUD (P > 0.05). In the latter, there were 85 cases, of whom, 30 with hypermenorrhea, 30 with menostaxis, and 25 with preceded menstrual cycle, the total effective rate was 90%, 93% and 88% respectively. After HA was used, the amount of the menstrual blood was significantly reduced, the menstrual period markedly shortened (P < 0.01), while the menstrual cycle was prolonged and hemoglobin increased significantly (P < 0.01). The results showed that HA is a good recipe in preventing and treating abnormal menstruation caused by applying IUD, with highly effective and safe, and without any side effects and contraindications.
- Published
- 1994
31. Relationship between radioprotective and neuromotor effects of S-2(3-aminopropyl-amino)ethylphosphorothioate (WR-2721) in mice.
- Author
-
Liu WF, Shih JH, Liu RF, Ma C, Lin CH, Liu CY, Chang CL, and Wu MT
- Subjects
- Amifostine administration & dosage, Amifostine pharmacology, Animals, Behavior, Animal drug effects, Dose-Response Relationship, Drug, Male, Mice, Mice, Inbred ICR, Motor Activity drug effects, Radiation-Protective Agents, Amifostine toxicity, Neuromuscular Junction drug effects, Organothiophosphorus Compounds toxicity
- Abstract
The radioprotective and neuromotor effects of WR-2721 were studied in male albino ICR strain mice. The protective activity was evaluated by graded doses of WR-2721 (50-400 mg/kg, IP) against whole body 60Co gamma irradiation at a single maximal lethal threshold dose rad (i.e., 1250 rad). The neuromotor effects of the drug were assayed by its action, at the same dose range as used in the protection assay, on spontaneous motor activity (SMA) and wire hanging performance (WHP). Drug doses were administered 30 min before radiation exposure and neurobehavioral testings. The results showed a dose-dependent increase in radioprotection and an inhibition in the neuromotor tasks. The radioprotective efficacy was seen at doses at which intrinsic neuromotor deficits were detected (100-400 mg/kg). A dose-related parallelism of protective efficacy and neurobehavioral toxicity (i.e., SMA inhibition and WHP disruption) was also observed. The present findings suggest that WR-2721 induces neuromotor dysfunctions along with its radioprotectivity.
- Published
- 1987
- Full Text
- View/download PDF
32. [Superficial keratectomy (report of 60 cases) (author's transl)].
- Author
-
Liu RF
- Subjects
- Cornea blood supply, Humans, Cornea surgery, Corneal Diseases surgery
- Published
- 1981
33. [Studies on the elimination of nithiocyamine from patients with schistosomiasis and its toxicities to the liver-bile system of rats].
- Author
-
Liu CX, Huang WT, Liu RF, Ye GZ, Xiang JJ, Zhang ZL, and Hu JC
- Subjects
- Adolescent, Adult, Aged, Animals, Diphenylamine analogs & derivatives, Diphenylamine toxicity, Female, Humans, Male, Middle Aged, Rats, Thiocyanates toxicity, Aniline Compounds metabolism, Bile drug effects, Diphenylamine metabolism, Isothiocyanates, Liver drug effects, Schistosomiasis metabolism, Schistosomicides metabolism, Thiocyanates metabolism
- Published
- 1983
34. Kinetic studies and structure-activity relationships of bispyridinium oximes as reactivators of acetylcholinesterase inhibited by organophosphorus compounds.
- Author
-
Su CT, Wang PH, Liu RF, Shih JH, Ma C, Lin CH, Liu CY, and Wu MT
- Subjects
- Acetylcholinesterase, Animals, Antidotes pharmacology, Binding Sites, Cattle, Kinetics, Phosphorylation, Structure-Activity Relationship, Cholinesterase Reactivators pharmacology, Organophosphorus Compounds toxicity, Oximes pharmacology
- Abstract
The kinetics of the reactivation of acetylcholinesterase inhibited by isopropyl methylphosphonofluoridate was studied. The reactivators used include nine bispyridinium monooximes and three bispyridinium dioximes. The dissociation constant (Kd) and the rate constant (k2) of dephosphorylation of the complex formed from the organophosphorus acetylcholinesterase (OP-AChE) and the oxime were measured. The reactivation parameters obtained from the in vitro kinetic studies were used to elucidate the structure-activity relationships. The hydrophobic property of a nonoxime substituent at the 3-position on the pyridinium ring can exert a positive effect on their binding affinity to OP-AChE. However, the rate constants (k2) of the nucleophilic displacement of OP-AChE by oximes depend negatively on these physical and structural factors of the oximes. The correlations of the in vivo antidotal efficacy (ED50) of these bispyridinium oximes have been analyzed with their pharmacological properties, e.g., reactivation potency, antimuscarinic activities, and antinicotinic activities. However, no satisfactory correlations were observed. It may be concluded that the detoxication mechanism of poisoning by isopropyl methylphosphonofluoridate is different from those of pinacolyl methylphosphonofluoridate and paraoxon.
- Published
- 1986
- Full Text
- View/download PDF
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