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LITAF, HHEX, and DUSP1 expression in mesenchymal stem cells from patients with psoriasis.
- Source :
-
Genetics and molecular research : GMR [Genet Mol Res] 2015 Dec 02; Vol. 14 (4), pp. 15793-801. Date of Electronic Publication: 2015 Dec 02. - Publication Year :
- 2015
-
Abstract
- Psoriasis is a common chronic relapsing inflammatory skin disease, in which mesenchymal stem cells (MSCs) have been hypothesized to play an important role in abnormal localized inflammation and vascular proliferation observed in skin lesions. Previous studies have revealed abnormal gene expression patterns, DNA methylation status, and cytokine secretion of MSCs in psoriatic skin lesions, as well as some gene expression abnormalities related to inflammation and angiogenesis. We further verified the gene and protein expressions of inflammation-related lipopolysaccharide-induced tumor necrosis factor-alpha transcription factor (LITAF), dual-specificity protein phosphatase 1 (DUSP1), and angiogenesis-related hematopoietically expressed homeobox (HHEX) in MSCs derived from the skin lesions of psoriasis patients. The gene expression of LITAF, DUSP1, and HHEX in dermal MSCs was measured at the mRNA level using reverse transcription-polymerase chain reaction and the corresponding protein expression levels were analyzed by western blotting analysis. The gene and protein expression levels of LITAF, HHEX, and DUSP1 in dermal MSCs were significantly lower in psoriasis patients compared to controls. Amplification and western blotting results were consistent with our previously reported gene chip data. Our results suggest that dermal MSCs in psoriatic skin lesions may be involved in the development, progression, and regulation of localized inflammatory abnormalities by reducing the expression of LITAF, HHEX, and DUSP1, which are related to inflammation and angiogenesis.
- Subjects :
- Adult
Case-Control Studies
Dual Specificity Phosphatase 1 metabolism
Female
Homeodomain Proteins metabolism
Humans
Male
Middle Aged
Nuclear Proteins metabolism
Psoriasis diagnosis
RNA, Messenger genetics
RNA, Messenger metabolism
Severity of Illness Index
Transcription Factors metabolism
Young Adult
Dual Specificity Phosphatase 1 genetics
Gene Expression
Homeodomain Proteins genetics
Mesenchymal Stem Cells metabolism
Nuclear Proteins genetics
Psoriasis genetics
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1676-5680
- Volume :
- 14
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Genetics and molecular research : GMR
- Publication Type :
- Academic Journal
- Accession number :
- 26634547
- Full Text :
- https://doi.org/10.4238/2015.December.1.31