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Research Note Mesenchymal stem cells from skin lesions of psoriasis patients promote proliferation and inhibit apoptosis of HaCaT cells.
- Source :
-
Genetics and molecular research : GMR [Genet Mol Res] 2015 Dec 22; Vol. 14 (4), pp. 17758-67. Date of Electronic Publication: 2015 Dec 22. - Publication Year :
- 2015
-
Abstract
- Psoriasis is an inflammatory skin disease characterized by excessive proliferation and abnormal differentiation and apoptosis of keratinocytes (KCs). Mesenchymal stem cells (MSCs) from skin lesions of psoriasis patients demonstrate abnormal cytokine secretion, which may affect KC proliferation and apoptosis. Here, we explored how MSCs from skin lesions of psoriasis patients affect HaCaT cell proliferation and apoptosis. First, flow cytometry and multipotent differentiation methods were used to identify skin MSCs, which were then co-cultured with HaCaT cells. HaCaT cell proliferation was analyzed in real-time, and cell cycle progression and apoptosis were assessed by flow cytometry. Cell morphologies and multipotencies of skin MSCs were similar between the psoriasis group and healthy control group, with high levels of CD29, CD44, CD73, CD90, and CD105 and limited expression of CD34, CD45, and HLA-DR. MSCs from skin lesions of psoriasis patients promote KC proliferation more potently and are less capable of inducing KC apoptosis. This may underlie KC proliferation and abnormal apoptosis in psoriasis skin lesions, which results in abnormal thickening of the epidermis.
- Subjects :
- Adult
Antigens, CD biosynthesis
Antigens, CD genetics
Apoptosis genetics
Cell Differentiation genetics
Cell Proliferation genetics
Epidermis metabolism
Epidermis pathology
Female
Flow Cytometry
Humans
Keratinocytes metabolism
Male
Mesenchymal Stem Cells metabolism
Middle Aged
Psoriasis pathology
Skin metabolism
Keratinocytes pathology
Mesenchymal Stem Cells pathology
Psoriasis genetics
Skin pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1676-5680
- Volume :
- 14
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Genetics and molecular research : GMR
- Publication Type :
- Academic Journal
- Accession number :
- 26782421
- Full Text :
- https://doi.org/10.4238/2015.December.21.49