107 results on '"Makoto Sudo"'
Search Results
2. Effect of Shot Composition, Continuity Assurance Method and Shot Size on Subjective Perception of Continuity during Transitions while Viewing Videos
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Makoto SUDO and Kiyoko YOKOYAMA
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- 2022
3. Postoperative remission of diabetes mellitus after gastrectomy in patients with diabetes mellitus and gastric cancer
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Akihito Mizukami, Yoshihiko Kawaguchi, Kastutoshi Shoda, Hidenori Akaike, Ryo Saito, Suguru Maruyama, Kensuke Shiraishi, Shinji Furuya, Hidetake Amemiya, Hiromichi Kawaida, Makoto Sudo, Hiroshi Kono, and Daisuke Ichikawa
- Abstract
Background/aim: We investigated the postoperative treatment status for diabetes mellitus and perioperative HbA1c levels in patients with diabetes mellitus and examined the effects of clinical factors on the remission of diabetes mellitus. Patients and methods: In this study, 126 patients with gastric cancer were considered to have diabetes mellitus preoperatively, of whom 79 were treated with oral antidiabetic drugs and/or insulin treatment. We compared diabetic treatment status and HbA1c values between the preoperative and postoperative periods in patients who underwent gastrectomy and examined the effects of clinical factors on improving diabetes mellitus. Results: Of the 79 patients treated preoperatively for diabetes mellitus, 34(43%) discontinued all medications for diabetes mellitus and 37 (47%) reduced the therapeutic dose or switched from insulin to oral antidiabetic drugs. Total gastrectomy was an independent factor for the remission of antidiabetic treatments after gastrectomy. Concerning the HbA1c level, only the absence of preoperative insulin use was an independent factor for the improvement. However, reconstruction was not a significant correlated factor for the improvement of postoperative HbA1c levels and the remission of antidiabetic medications after distal gastrectomy. Conclusions: Almost all patients discontinued or reduced the dose of antidiabetic medications after gastrectomy in clinical practice, and special attention should be paid in the management methods for diabetes mellitus in patients who underwent total gastrectomy for gastric cancer.
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- 2023
4. Clinical Significance of Past History of Breast Cancer Screening for the Prognosis of Triple Negative Breast Cancer
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Yoshihiko Kawaguchi, Katsutoshi Shoda, Shingo Inoue, Naohiro Hosomura, Hidetake Amemiya, Makoto Sudo, Masato Ohmori, Hidenori Akaike, Ayako Kimura, Ryo Saito, Hiromichi Kawaida, Yuko Nakayama, Daisuke Ichikawa, Shinji Furuya, and Hiroshi Kono
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Adult ,Oncology ,Cancer Research ,medicine.medical_specialty ,Prognostic factor ,Multivariate analysis ,medicine.medical_treatment ,Triple Negative Breast Neoplasms ,Breast cancer screening ,Drug Therapy ,Internal medicine ,Overall survival ,medicine ,Humans ,Clinical significance ,Early Detection of Cancer ,Triple-negative breast cancer ,Aged ,Chemotherapy ,medicine.diagnostic_test ,business.industry ,General Medicine ,Middle Aged ,Prognosis ,Survival Analysis ,Past history ,Lymphatic Metastasis ,Female ,business - Abstract
BACKGROUND/AIM This study examined the prognostic impact of the past history of breast cancer screening within the last 2 years (PH-BCS), for patients with triple negative breast cancer (TNBC), a subtype that carries extremely poor prognosis. PATIENTS AND METHODS Eighty-six consecutive cases with TNBC, who underwent surgery at our faculty from 2009 to 2015, were divided into two groups according to PH-BCS. Prognostic analyses for disease-free survival and overall survival between the two groups were performed. RESULTS The positive PH-BCS group (n=44) had a significantly better prognoses than the negative PH-BCS group (n=42) (p
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- 2021
5. Impact of Insulin Treatment on Prognosis of non-B non-C Hepatocellular Carcinoma After Hepatectomy
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Daisuke Ichikawa, Ryo Saito, Hiroshi Kono, Naohiro Hosomura, Makoto Sudo, Hiromichi Kawaida, Hidenori Akaike, Yoshihiko Kawaguchi, Masanori Matsuda, and Hidetake Amemiya
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Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Survival ,medicine.medical_treatment ,Hepatitis C virus ,Insulins ,medicine.disease_cause ,Gastroenterology ,Insulin resistance ,Diabetes mellitus ,Internal medicine ,Diabetes Mellitus ,medicine ,Hepatectomy ,Humans ,Aged ,Postoperative Care ,Hepatitis B virus ,business.industry ,Insulin ,Liver Neoplasms ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,digestive system diseases ,Oncology ,Hepatocellular carcinoma ,Female ,Disease Susceptibility ,business ,Viral hepatitis - Abstract
Background/aim Several studies have reported that DM is closely associated with an increased incidence of hepatocellular carcinoma (HCC). To clarify the effects of diabetes mellitus (DM) and antidiabetic medications on the prognosis of patients with non-B non-C (NBNC) HCC following curative initial hepatectomy. Patients and methods HCC patients (n=156) were divided into three groups according to the presence or absence of chronic viral hepatitis: hepatitis B virus (HBV) group, hepatitis C virus (HCV) group, and NBNC group. The clinical characteristics and survival outcomes were compared. In the NBNC group, univariate and multivariate analyses were conducted to determine prognostic factors. Results The NBNC group had a higher incidence of DM, ethanol intake, and large nodules than the other groups. Disease-free survival (DFS) was significantly worse in the NBNC group than in the HBV group. In the NBNC group, insulin treatment was an independent prognostic factor for DFS and overall survival (OS). Conclusion Medications for DM that affect insulin resistance might be appropriate prognostic factors for NBNC-HCC.
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- 2021
6. Early Drain Removal Regardless of Drain Fluid Amylase Level Might Reduce Risk of Postoperative Pancreatic Fistula
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Hidenori Akaike, Shinji Furuya, Jun Itakura, Daisuke Ichikawa, Katsutoshi Shoda, Yoshihiko Kawaguchi, Takashi Nakayama, Naohiro Hosomura, Hiromichi Kawaida, Hidetake Amemiya, Yuuki Nakata, Hiroki Shimizu, Yudai Higuchi, Isamu Tsukahara, Naoki Ashizawa, Makoto Sudo, Hideki Fujii, Hiroshi Kono, and Ryo Saito
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Cancer Research ,medicine.medical_specialty ,Time Factors ,Abdominal cavity ,Gastroenterology ,Pancreatic Fistula ,03 medical and health sciences ,Pancreatectomy ,Postoperative Complications ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Humans ,Medicine ,In patient ,Amylase ,Device Removal ,Postoperative Care ,biology ,business.industry ,Incidence ,Disease Management ,General Medicine ,medicine.disease ,medicine.anatomical_structure ,Point of delivery ,ROC Curve ,Oncology ,Pancreatic fistula ,030220 oncology & carcinogenesis ,Amylases ,Pancreatic juice ,biology.protein ,Drainage ,Drain removal ,business ,Distal pancreatectomy ,Biomarkers - Abstract
BACKGROUND/AIM Drains are frequently placed at the time of distal pancreatectomy (DP) to evacuate pancreatic juice and intra-abdominal exudate and obtain information on abdominal cavity status. However, the timing of drain removal remains debatable. Meanwhile, prolonged drain placement might increase the risk of postoperative pancreatic fistula (POPF), with a prevalence of 5-40%. Therefore, we examined the effect of removing the drain within postoperative day (POD) 3 on the risk of POPF development. PATIENTS AND METHODS A total of 108 consecutive patients who underwent DP between April 2015 and March 2020 were examined and divided into two groups according to the day of drain removal; hence, for some patients, the drain was removed on POD 1 (POD 1 group) and for others on POD 3 (POD 3 group). Furthermore, risk factors, including drain fluid amylase (DFA) levels, for developing POPF were investigated. RESULTS The overall rate of clinically relevant POPF was 4.6% and did not significantly differ between the POD 1 and POD 3 groups [4.5% and 4.9%, respectively (p=0.924)]. DFA levels on POD 1 did not significantly differ between patients with and without POPF. On POD 3 and POD 5, C-reactive protein (CRP) levels were significantly higher in patients with POPF than in those without (p=0.03 and p
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- 2021
7. A Case of Non-tumor Forming and Anal Gland Originating Anal Canal Cancer with Pagetoid Spread
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Shugo Shiba, Michio Hara, Makoto Sudo, Daisuke Ichikawa, Hirotaka Okamoto, and Hiroshi Iino
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business.industry ,Pagetoid ,Anal gland ,General Engineering ,General Earth and Planetary Sciences ,Medicine ,Anatomy ,Anal Canal Cancer ,business ,General Environmental Science - Published
- 2021
8. Platelets enhance malignant behaviours of gastric cancer cells via direct contacts
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Shinji Furuya, Ryo Saito, Hidenori Akaike, Suguru Maruyama, Katsutoshi Shoda, Makoto Sudo, Naohiro Hosomura, Hidetake Amemiya, Hiromichi Kawaida, Koichi Takiguchi, Shingo Inoue, Yoshihiko Kawaguchi, Hiroshi Kono, Daisuke Ichikawa, Katsue Suzuki-Inoue, and Atsushi Yamamoto
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Blood Platelets ,Cancer microenvironment ,Cancer Research ,Epithelial-Mesenchymal Transition ,Microarray ,Cell ,Cell Communication ,Brief Communication ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Western blot ,Cell Movement ,Stomach Neoplasms ,Cell Adhesion ,medicine ,Humans ,Neoplasm Invasiveness ,Platelet ,Gene ,Cell Proliferation ,medicine.diagnostic_test ,Chemistry ,Cancer ,medicine.disease ,Coculture Techniques ,Up-Regulation ,medicine.anatomical_structure ,Matrix Metalloproteinase 9 ,Oncology ,Tissue Array Analysis ,030220 oncology & carcinogenesis ,Cancer cell ,Disease Progression ,Cancer research ,Gastric cancer ,Signal Transduction - Abstract
In this study, we aimed to analyse human cancer cell–platelet interactions in functional cell analyses and explore the molecular mechanisms behind tumour progression. Various functional analyses of gastric cancer (GC) cells were performed after direct/indirect co-incubation with platelets derived from GC patients. Further detailed expression and signalling analyses were performed after co-culture with direct and indirect GC cells–platelet contact. Malignant behaviours of cancer cells, such as proliferation, migration, invasion and adhesion, were significantly enhanced after direct co-incubation with platelets. Microarray analyses demonstrated changes in multiple genes, including epithelial–mesenchymal transition (EMT)-related genes. Among them, matrix metalloproteinase 9 was notably upregulated, which was validated by quantitative reverse transcription–polymerase chain reaction and western blot. Further, this change was only observed after direct co-incubation with platelets. This study demonstrated that platelets from GC patients promote malignant behaviours of GC cells through EMT-related signalling, especially by direct contact with tumour cells.
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- 2020
9. Liquid biopsy as a perioperative biomarker of digestive tract cancers: review of the literature
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Daisuke Ichikawa, Hidenori Akaike, Hiromichi Kawaida, Yoshihiko Kawaguchi, Shinji Furuya, Makoto Sudo, Hidetake Amemiya, Suguru Maruyama, Shingo Inoue, Katsutoshi Shoda, Hiroshi Kono, and Ryo Saito
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Oncology ,medicine.medical_specialty ,Digestive System Neoplasms ,03 medical and health sciences ,0302 clinical medicine ,Circulating tumor cell ,Internal medicine ,Biopsy ,Biomarkers, Tumor ,medicine ,Humans ,Sampling (medicine) ,Molecular Targeted Therapy ,Precision Medicine ,Liquid biopsy ,Perioperative Period ,Monitoring, Physiologic ,medicine.diagnostic_test ,business.industry ,Patient Selection ,Liquid Biopsy ,Cancer ,General Medicine ,Prognosis ,Precision medicine ,medicine.disease ,Body Fluids ,030220 oncology & carcinogenesis ,Biomarker (medicine) ,030211 gastroenterology & hepatology ,Surgery ,Personalized medicine ,Neoplasm Recurrence, Local ,business - Abstract
Tissue biopsies are the gold-standard for investigating the molecular characterization of tumors. However, a "solid" biopsy is an invasive procedure that cannot capture real-time tumor dynamics and may yield inaccurate information because of intratumoral heterogeneity. In this review, we summarize the current state of knowledge about surgical treatment-associated "liquid" biopsy for patients with digestive organ tumors. A liquid biopsy is a technique involving the sampling and testing of non-solid biological materials, including blood, urine, saliva, and ascites. Previous studies have reported the potential value of blood-based biomarkers, circulating tumor cells, and cell-free nucleic acids as facilitators of cancer treatment. The applications of a liquid biopsy in a cancer treatment setting include screening and early diagnosis, prognostication, and outcome and recurrence monitoring of cancer. This technique has also been suggested as a useful tool in personalized medicine. The transition to precision medicine is still in its early stages. Soon, however, liquid biopsy is likely to form the basis of patient selection for molecular targeted therapies, predictions regarding chemotherapy sensitivity, and real-time evaluations of therapeutic effects.
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- 2020
10. Is Intraluminal Washout Necessary for Patients with Sigmoid Colon Cancer to Eliminate Exfoliated Cancer Cells as in Patients with Rectal Cancer? A Pilot Study at a Single Institute
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Yoshihiko Kawaguchi, Shinji Furuya, Hiroki Shimizu, Suguru Maruyama, Tetsuo Kondo, Daisuke Ichikawa, Ryo Saito, Makoto Sudo, Hiromichi Kawaida, and Koichi Takiguchi
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medicine.medical_specialty ,sigmoid colon cancer ,business.industry ,Colorectal cancer ,medicine.medical_treatment ,Urology ,Papanicolaou stain ,Washout ,Anastomosis ,medicine.disease ,law.invention ,Sigmoidectomy ,Randomized controlled trial ,intraluminal washout ,law ,Cancer cell ,medicine ,lcsh:Diseases of the digestive system. Gastroenterology ,Original Research Article ,distal margin ,lcsh:RC799-869 ,rectal cancer ,business ,exfoliated cancer cells ,Saline - Abstract
Objectives: Rectal stump washout has been widely performed to prevent the implantation of exfoliated cancer cells (ECCs) in patients with rectal cancer. However, it remains unclear whether intraluminal washout before transection is required in patients with sigmoid colon cancer. Therefore, this pilot study was conducted to elucidate the necessity of intraluminal washout for sigmoid colon cancer patients in comparison with rectal cancer patients by cytological assessments. Methods: A total of 16 patients with sigmoid colon cancer and 24 patients with rectal cancer who underwent sigmoidectomy or anterior resection with anastomosis using double-stapling technique were enrolled. A transanal washout sample was collected before washout and after irrigation with 500 and 1,000 mL of saline. Cytological assessments were conducted according to the Papanicolaou classification, and class IV and V cells were defined as malignant. Results: Before washout, exfoliated cancer cells were found in 15 of 24 (62.5%) patients with rectal cancer and in 1 of 16 (6.2%) patients with sigmoid colon cancer (p < 0.001). Distal-free margin from the tumor was significantly shorter in patients with cancer cells (p = 0.002), and the length of the distal-free margin was significantly associated with the tumor location. After irrigation with 500 and 1,000 mL of saline, no cancer cell was found in all patients with sigmoid colon cancer, whereas ECCs were still found in five patients with rectal cancer (20.8%). Conclusions: Intraluminal washout with 1,000 mL may be sufficient for sigmoid colon cancer patients with longer distal-free margin. A large-scale, randomized controlled study is necessary to confirm these results.
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- 2020
11. Clinical Significance of Dynamic Neutrophil-lymphocyte Ratio Changes in Patients With Colorectal Cancer
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Shoda Katsutoshi, Shingo Inoue, Makoto Sudo, Yoshihiko Kawaguchi, Hiromichi Kawaida, Shinji Furuya, Naoki Ashizawa, Hidenori Akaike, Naohiro Hosomura, Daisuke Ichikawa, Hiroshi Kono, and Hidetake Amemiya
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Male ,Oncology ,Cancer Research ,Prognostic factor ,medicine.medical_specialty ,Multivariate analysis ,Neutrophils ,Colorectal cancer ,Lymphocyte ,Kaplan-Meier Estimate ,Stage ii ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,In patient ,Clinical significance ,Lymphocyte Count ,Lymphocytes ,neoplasms ,Aged ,Neoplasm Staging ,Retrospective Studies ,Aged, 80 and over ,business.industry ,fungi ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Multivariate Analysis ,Preoperative Period ,Female ,Colorectal Neoplasms ,business - Abstract
BACKGROUND/AIM Elevated neutrophil-lymphocyte ratio (NLR) has been reported to be a poor prognostic factor in patients with colorectal cancer (CRC). However, no studies have focused on the dynamic change of preoperative NLR (pre-NLR) in CRC patients. We investigated the prognostic value of the change in NLR (ΔNLR) in CRC patients before and after surgery. PATIENTS AND METHODS We retrospectively analyzed the data from 307 patients with stage II or III CRC. We compared the clinicopathological factors, OS, and DFS among the various NLR factors. RESULTS The 5-year OS rate of the high ΔNLR group was significantly lower than that of the low ΔNLR group (p
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- 2020
12. Postoperative Complications have Minimal Impact on Long-Term Prognosis in Immunodeficient Patients with Esophageal Cancer
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Hiromichi Kawaida, Ryo Saito, Makoto Sudo, Yoshihiko Kawaguchi, Hiroki Shimizu, Katsutoshi Shoda, Hidetake Amemiya, Shingo Inoue, Naohiro Hosomura, Hiroshi Kono, Hidenori Akaike, Suguru Maruyama, Daisuke Ichikawa, and Shinji Furuya
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medicine.medical_specialty ,Esophageal Neoplasms ,medicine.medical_treatment ,Splenectomy ,03 medical and health sciences ,Postoperative Complications ,0302 clinical medicine ,Gastrectomy ,Stomach Neoplasms ,Surgical oncology ,medicine ,Humans ,In patient ,Retrospective Studies ,business.industry ,Cancer ,Retrospective cohort study ,Immunosuppression ,Esophageal cancer ,Prognosis ,medicine.disease ,Surgery ,Esophagectomy ,Survival Rate ,Oncology ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,business - Abstract
Postoperative complications have been recognized to have an adverse prognostic impact in various types of cancer. However, in a recent study, it has been reported that postoperative complications of total gastrectomy with splenectomy have little impact on the long-term outcomes of patients with gastric cancer. In addition, the mechanisms underlying the effect of postoperative complications on outcomes remain to be elucidated. We hypothesized that immunosuppression by postoperative complications may affect long-term outcomes in patients with esophageal cancer. In this retrospective study, we assessed in 153 patients with esophageal cancer who underwent curative subtotal esophagectomy at our hospital and examined the correlation between postoperative complications, and multiple clinicopathological factors, and long-term outcomes with the patients stratified by total lymphocyte count (TLC). The median preoperative TLC was 1432. A total of 115 patients (75.2%) had a TLC of ≥ 1000/μL (high TLC group), and the remaining 38 patients (24.8%) had a TLC of
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- 2020
13. Prognostic Significance of Treatment Strategies for the Recurrent Hepatocellular Carcinomas After Radical Resection
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Hiroki Shimizu, Naohiro Hosomura, Hiroshi Kono, Ryo Saito, Shingo Inoue, Yoshihiko Kawaguchi, Hiromichi Kawaida, Suguru Maruyama, Hidetake Amemiya, Daiduke Ichikawa, Shinji Furuya, Makoto Sudo, and Hidenori Akaike
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Male ,Cancer Research ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Radiofrequency ablation ,medicine.medical_treatment ,Gastroenterology ,General Biochemistry, Genetics and Molecular Biology ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,law ,Internal medicine ,Hepatectomy ,Humans ,Medicine ,Transcatheter arterial chemoembolization ,Survival rate ,Aged ,Neoplasm Staging ,Aged, 80 and over ,Pharmacology ,High rate ,business.industry ,Liver Neoplasms ,Disease Management ,Middle Aged ,Prognosis ,medicine.disease ,Combined Modality Therapy ,digestive system diseases ,Tumor Burden ,Survival Rate ,Treatment Outcome ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Treatment strategy ,Female ,Neoplasm Recurrence, Local ,business ,Radical resection ,Research Article - Abstract
Background/aim The high rate of recurrence and repetitive features of hepatocellular carcinoma (HCC) require specific treatment strategies. This study aimed to evaluate the long-term outcomes of recurrent HCC focusing on clinicopathological factors. Patients and methods A total of 104 patients who were treated with re-hepatectomy, radiofrequency ablation (RFA) or transcatheter arterial chemoembolization for recurrent HCC were analyzed. Post-recurrent prognoses were compared between each treatment group based on the presence of adverse prognostic factors (APFs) identified. Results In the hepatectomy group, the prognosis of patients with APFs was significantly worse compared to those without APFs. By contrast, the survival rate of patients who underwent RFA was not significantly different from those with and without APFs. Conclusion Our results demonstrate the heterogeneity that exists in terms of the long-term survival of patients with recurrent HCC. The treatment strategy for recurrent HCC should be based on the assessment of presence of APFs to improve long-term prognosis.
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- 2020
14. Stratification of Prognosis in Patients With Ampullary Carcinoma After Surgery by Preoperative Platelet-to-lymphocyte Ratio and Conventional Tumor Markers
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Tadao Nakazawa, Shinji Furuya, Hidetake Amemiya, Mitsuaki Watanabe, Shinichi Takano, Hiroki Shimizu, Hideki Fujii, Mitsuharu Fukasawa, Ryo Saito, Yoshihiko Kawaguchi, Hiroshi Kono, Suguru Maruyama, Nobuyuki Enomoto, Erika Takahashi, Jun Itakura, Daisuke Ichikawa, Naohiro Hosomura, Masanori Matusda, Makoto Sudo, Hidenori Akaike, Tadashi Satou, Hiroko Shindo, and Hiromichi Kawaida
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Male ,Ampulla of Vater ,Cancer Research ,Poor prognosis ,medicine.medical_specialty ,medicine.medical_treatment ,Lymphocyte ,Gastroenterology ,Pancreaticoduodenectomy ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Biomarkers, Tumor ,medicine ,Carcinoma ,Humans ,Platelet ,In patient ,Lymphocyte Count ,Aged ,Tumor marker ,Ampullary carcinoma ,Platelet Count ,business.industry ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Survival Analysis ,Pancreatic Neoplasms ,body regions ,Treatment Outcome ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Female ,business ,Carcinoma, Pancreatic Ductal - Abstract
Background/aim The platelet-to-lymphocyte ratio (PLR) has recently been suggested as a new predictor of the prognosis in several carcinoma types. However, the clinical impact remains controversial in patients with ampullary carcinoma. Thus, the aim of this study was to investigate other useful biomarkers for identifying poor prognosis in patients with ampullary carcinoma. Patients and methods Forty-one patients with ampullary carcinoma underwent pancreaticoduodenectomy (PD) with curative resection between April 2000 and April 2017. Various clinicopathological findings of the patients and their tumors were evaluated as potential prognostic factors which might enable better stratification of prognosis. Results Platelet-to-lymphocyte ratio, as well as other markers, was found to be a prognostic factor in patients with ampullary carcinoma. The 2-year disease-free survival percentage was significantly higher in the group with low PLR than in the high PLR group (70.2% vs. 28.6%; p=0.005). Combinational analysis of the PLR and conventional TMs enabled us to stratify prognosis of the patients more clearly than by each marker alone. Conclusion PLR was a useful prognostic factor for patients with ampullary cancer. The combination of preoperative PLR and conventional TMs markers may be powerful predictive factors for postoperative prognosis in patients with ampullary carcinoma following PD.
- Published
- 2019
15. Differential miRNA Expression in Basaloid Squamous Cell Carcinoma of the Oesophagus: miR-3687 Targets PGRMC2
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Makoto Sudo, Hiroki Shimizu, Hidenori Akaike, Yoshihiko Kawaguchi, Naohiro Hosomura, Hidetake Amemiya, Jiro Nakamura, Hiroshi Kono, Shinji Furuya, Daisuke Ichikawa, Suguru Maruyama, Kotaro Hagio, Hiromichi Kawaida, and Kensuke Shiraishi
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Cancer Research ,Messenger RNA ,Cell growth ,General Medicine ,Transfection ,Biology ,Reverse transcription polymerase chain reaction ,03 medical and health sciences ,0302 clinical medicine ,Oncology ,030220 oncology & carcinogenesis ,microRNA ,Progesterone receptor ,Cancer research ,Basaloid Squamous Cell Carcinoma ,Gene - Abstract
Background/aim Basaloid squamous cell carcinoma of the oesophagus (BSCCE) has poorer prognosis than conventional oesophageal squamous cell carcinoma (ESCC). This study is the first report on highly expressed miRNAs in BSCCE and their target genes. Materials and methods BSCCE and ESCC patients who underwent esophagectomy were selected for this study. Total RNA was extracted from formalin-fixed paraffin-embedded blocks to examine expression of miRNAs and target genes. miRNA mimic or inhibitor transfected cells were used in validation experiments. miRNA and mRNA quantification were performed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results miRNA microarray analysis revealed four candidate miRNAs. Further investigations including cell line experiments demonstrated that miR-3687 was a candidate miRNA and progesterone receptor membrane component2 (PGRMC2) was its target gene. PGRMC2 was found to be related to cell proliferation and local progression. Conclusion miR-3687 may be a candidate miRNA conferring BSCCE aggressiveness, and PGRMC2 is one of its target genes.
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- 2019
16. Cytoglobin as a Prognostic Factor for Pancreatic Ductal Adenocarcinoma: A Retrospective Analysis of 75 Patients
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Makoto Sudo, Naohiro Hosomura, Shinji Furuya, Hidenori Akaike, Hiroshi Kono, Daisuke Ichikawa, Hiromichi Kawaida, Hidetake Amemiya, and Yoshihiko Kawaguchi
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Male ,Vascular Endothelial Growth Factor A ,Lymphovascular invasion ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Kaplan-Meier Estimate ,chemistry.chemical_compound ,Phosphatidylinositol 3-Kinases ,Endocrinology ,Internal Medicine ,Carcinoma ,Medicine ,Humans ,Stage (cooking) ,Aged ,Neoplasm Staging ,Retrospective Studies ,Aged, 80 and over ,Univariate analysis ,Hepatology ,business.industry ,Interleukin-6 ,Cytoglobin ,Middle Aged ,medicine.disease ,Prognosis ,Immunohistochemistry ,Vascular endothelial growth factor ,Pancreatic Neoplasms ,chemistry ,Pancreatectomy ,Multivariate Analysis ,Cancer research ,Female ,business ,Proto-Oncogene Proteins c-akt ,Carcinoma, Pancreatic Ductal - Abstract
Objectives The aim was to evaluate the relationship between cytoglobin (Cygb) expression and both clinicopathologic factors and prognosis in patients with pancreatic ductal adenocarcinoma (PDAC). Methods Seventy-five patients with PDAC who underwent pancreatectomy between 2009 and 2014 at our department were included. Diagnosis was based on World Health Organization standards, with staging by TNM classification of Union for International Cancer Control. Expressions of Cygb, phosphoinositide-3 kinase, phosphorylated protein kinase B, interleukin-6, and vascular endothelial growth factor were evaluated by immunohistochemical staining of resected surgical specimens and densitometrical analysis. Results Elevated expression of Cygb was found mainly in carcinoma cells of PDAC. Patients with low expression of Cygb showed significantly shorter disease-free survival and disease-specific survival than those with high expression. There was also a significant negative correlation between Cygb expression and the expressions of phosphoinositide 3-kinase, phosphorylated protein kinase B, interleukin-6, and vascular endothelial growth factor. In univariate analysis, Cygb expression, clinical stage, histologic tumor grade, lymphatic invasion, and vascular invasion were prognostic factors. In multivariate analysis, Cygb expression and the clinical stage were independent prognostic factors. Conclusions Loss of Cygb may contribute to tumor recurrence and poor prognosis of PDAC by increases in angiogenic factor.
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- 2021
17. 335 DIFFERENTIALLY EXPRESSED MICRORNAS AND PREDICTIVE TARGET GENES IN BASALOID SQUAMOUS CELL CARCINOMA OF THE ESOPHAGUS
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Hiroki Shimizu, Shinji Furuya, Jiro Nakamura, Hiroshi Kono, Kotaro Hagio, Daisuke Ichikawa, Naohiro Hosomura, Hidenori Akaike, Suguru Maruyama, Makoto Sudo, Hiromichi Kawaida, Yoshihiko Kawaguchi, Kensuke Shiraishi, and Hidetake Amemiya
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medicine.anatomical_structure ,business.industry ,microRNA ,Gastroenterology ,Cancer research ,Medicine ,General Medicine ,Esophagus ,Basaloid Squamous Cell Carcinoma ,business ,Gene - Abstract
Background/Aim: Esophageal cancer is the sixth leading cause of cancer-related deaths worldwide. Esophageal squamous cell carcinoma (ESCC) is considered as one of the most aggressive carcinomas of the gastrointestinal tract. Basaloid squamous cell carcinoma of the esophagus (BSCCE) is reported to have a poorer prognosis compared to conventional ESCC. The current study aimed to elucidate molecular differences between BSCCE and ESCC, using miRNA profiling and predictive target gene searching. Methods Materials and Methods: Four BSCCE and 94 ESCC patients who underwent esophagectomy were selected for this study. Cell lines were used for target gene validation. Total RNA samples, extracted from formalin-fixed paraffin-embedded blocks, were used for microarray profiling and validation of the miRNAs, selecting the candidate target genes, and elucidating their clinicopathological features. Furthermore, total RNA samples, extracted from miRNA mimic- and inhibitor-transfected cells in cell line experiments, were used for target gene validation. Both miRNA and mRNA quantifications were performed by quantitative reverse transcription-polymerase chain reaction. Results The microarray analysis revealed seven highly expressed miRNAs (miR-205-5p, −4732-5p, −1246, −3687, −3175, −6087, and − 1587) in the BSCCE patients when compared with control. We selected miR-4732-5p and − 3687 for the validation study, and target gene investigations were conducted for miR-3687 ultimately. Several candidates were selected after searching for the target genes via TargetScan and in the literature. Through a pilot and a validation study, progesterone receptor membrane component 2 (PGRMC2) was identified as a target gene. Further investigations revealed that PGRMC2 was associated with tumor size clinicopathologically. Conclusion miR-3687 may constitute a candidate marker of aggressiveness in BSCCE, and PGRMC2 is one of its target genes. Moreover, the gene may play a role in cell proliferation and local progression. Although the current study included only a small number of samples, this is the first report regarding differentially expressed miRNAs and predictive target genes in BSCCE patients.
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- 2021
18. NADPH oxidase 5 has a crucial role in cellular motility of colon cancer cells
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Hidetake Amemiya, Daisuke Ichikawa, Hiroki Shimizu, Shinji Furuya, Keita Katsurahara, Shingo Inoue, Atsushi Shiozaki, Makoto Sudo, Hidenori Akaike, Hiroshi Kono, Katsutoshi Shoda, Naohiro Hosomura, Hiromichi Kawaida, Yoshihiko Kawaguchi, and Naoki Ashizawa
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Male ,Cancer Research ,Colorectal cancer ,Cell ,Biology ,Cell Movement ,Cell Line, Tumor ,medicine ,Humans ,Aged ,Cell Proliferation ,Oligonucleotide Array Sequence Analysis ,Gene knockdown ,Oncogene ,Cell growth ,Gene Expression Profiling ,Cancer ,Cell migration ,Cell cycle ,HCT116 Cells ,medicine.disease ,digestive system diseases ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,medicine.anatomical_structure ,NADPH Oxidase 5 ,Oncology ,Colonic Neoplasms ,Disease Progression ,Cancer research ,Signal Transduction - Abstract
NADPH oxidases (NOXs) are a family of transmembrane proteins that generate reactive oxygen species. It was previously reported that patients with colon cancer who had high NOX5 expression had poor prognosis. However, no studies have investigated the cellular functions of NOX5 in colon cancer. The present study aimed to clarify the relationship between NOX5 and cancer development using an in vitro model. Reverse transcription‑quantitative PCR was performed to determine the NOX5 expression levels of colon cancer cell lines. NOX5‑knockdown experiments were conducted, and the effect on cell proliferation, migration, and invasion were analyzed. In addition, mRNA microarray was conducted to assess changes in gene profile. NOX5 mRNA expression was high in HCT116 cells and moderate in SW48 cells. NOX5 knockdown significantly inhibited cell migration and invasion in both HCT116 and SW48 cells; however, NOX5 knockdown reduced cell proliferation in only HCT116 cells. mRNA microarrays revealed a strong relationship between NOX5 expression levels and integrin‑linked kinase signaling pathways. The NOX5 expression in colon cancer cells affected cancer progression, especially cell motility. NOX5 may be a novel therapeutic target for the future development of treatments for colon cancer.
- Published
- 2021
19. Prognostic Significance of Hiatal Hernia in Patients with Gastric Cancer Located within the Upper-Third of the Stomach
- Author
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Suguru Maruyama, Hiroki Shimizu, Shingo Inoue, Hiromichi Kawaida, Ryo Saito, Shinji Furuya, Hidenori Akaike, Yoshihiko Kawaguchi, Hidetake Amemiya, Naohiro Hosomura, Daisuke Ichikawa, Makoto Sudo, Hiroshi Kono, and Kensuke Shiraishi
- Subjects
Adult ,Male ,medicine.medical_specialty ,030230 surgery ,Gastroenterology ,Hiatal hernia ,03 medical and health sciences ,0302 clinical medicine ,Stomach Neoplasms ,Internal medicine ,Humans ,Medicine ,Clinical significance ,Hernia ,Stage (cooking) ,Lymph node ,Aged ,Retrospective Studies ,business.industry ,Stomach ,Cancer ,Middle Aged ,Prognosis ,medicine.disease ,Hernia, Hiatal ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Female ,Surgery ,Esophagogastric Junction ,business ,Abdominal surgery - Abstract
Gastric cancers located within the upper-third of the stomach (UGC), especially the esophagogastric junction GC (EGJGC), have distinct clinicopathological features due to their potential for multidirectional lymphatic spread. In this study, we investigated the clinical significance of hiatal hernias (HH) in patients with UGC, including EGJGC. In this retrospective study, we assessed status of HH in 147 patients with UGC who underwent curative resection at our hospital and examined the correlation between the presence of HH (+) and multiple clinicopathological factors. Thirty-four patients (23%) were HH (+). However, we found no significant correlation between HH (+) and clinicopathological factors. HH (+) patients frequently developed lymph node recurrences. Prognosis was significantly better in patients with UGC and HH (−), compared to those with UGC and HH (+). Similarly, EGJGC patients who were HH (−) showed superior survival compared to HH (+) patients. Multivariate analysis found that the HH (+) (p = 0.004), histological type (p = 0.029), and nodal stage (p = 0.034) were independent prognostic factors. The presence of HH might affect lymphatic spread of tumor cells, and consequently prognosis of patients with UGC. Therefore, special attention is needed in developing surgical and postoperative strategies for such patients with UGC who are HH (+).
- Published
- 2019
20. Clinical Significance of NADPH Oxidase 5 in Human Colon Cancer
- Author
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Daisuke Ichikawa, Hidenori Akaike, Hiromichi Kawaida, Yoshihiko Kawaguchi, Naoki Ashizawa, Shinji Furuya, Makoto Sudo, Hiroki Shimizu, Hidetake Amemiya, Shingo Inoue, Naohiro Hosomura, and Hiroshi Kono
- Subjects
Male ,Cancer Research ,Colorectal cancer ,03 medical and health sciences ,0302 clinical medicine ,Biomarkers, Tumor ,medicine ,Humans ,Clinical significance ,Survival rate ,Aged ,Neoplasm Staging ,business.industry ,Histology ,General Medicine ,Prognosis ,medicine.disease ,Progression-Free Survival ,Gene Expression Regulation, Neoplastic ,Human colon cancer ,NADPH Oxidase 5 ,Oncology ,Tumor progression ,030220 oncology & carcinogenesis ,Colonic Neoplasms ,NADPH oxidase 5 ,Cancer research ,Immunohistochemistry ,Female ,Neoplasm Recurrence, Local ,business - Abstract
Background/aim Recent studies have reported the involvement of NADPH oxidases (NOXs) in tumor progression. However, the role of NOX5 in colon cancer is unclear. We examined the clinical significance of NOX5 expression in colon cancer. Patients and methods NOX5 expression was evaluated by immunohistochemistry in 119 patients with stage II or III colon cancer, and the relationship between NOX5 expression and clinicopathological data was analyzed. Results Of all tissues, 39.5% were negative and 60.5% were positive for NOX5 expression. Positive expression was significantly associated with undifferentiated histology (p=0.037) and lymph node metastasis (p=0.023). The 5-year progression-free survival rate of NOX5-positive patients was significantly worse than that of NOX5-negative patients (p=0.046). The rates of local recurrence observed in NOX5-positive patients were higher than that in NOX5-negative patients. Conclusion NOX5 expression may be related to poor prognostic factors and could be useful as a prognostic biomarker.
- Published
- 2019
21. Prognostic Factors for Post-recurrent Survival in Hepatocellular Carcinoma After Curative Resection
- Author
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Hidenori Akaike, Shinji Furuya, Shingo Inoue, Yoshihiko Kawaguchi, Makoto Sudo, Hiroki Shimizu, Naohiro Hosomura, Suguru Maruyama, Ryo Saito, Daisuke Ichikawa, Hiroshi Kono, Hidetake Amemiya, and Hiromichi Kawaida
- Subjects
Male ,Curative resection ,Oncology ,Cancer Research ,medicine.medical_specialty ,Poor prognosis ,Carcinoma, Hepatocellular ,Time Factors ,Multivariate analysis ,medicine.medical_treatment ,Clinical Decision-Making ,Risk Assessment ,Decision Support Techniques ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,medicine ,Hepatectomy ,Humans ,Surgical treatment ,Aged ,Retrospective Studies ,business.industry ,Liver Neoplasms ,General Medicine ,medicine.disease ,Recurrent Hepatocellular Carcinoma ,Clinical Practice ,Treatment Outcome ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Female ,Neoplasm Recurrence, Local ,business ,Algorithms - Abstract
BACKGROUND/AIM Treatment algorithms for primary and recurrent hepatocellular carcinoma (HCC) are described in the current Japanese Clinical Practice Guidelines; however, primary and recurrent tumors exhibit several differences in oncological characteristics such as clinicopathological features and prognostic factors. This study aimed to investigate the prognostic factors for recurrent HCC including time of recurrence after primary hepatectomy, to elucidate appropriate treatment strategies in these patients. PATIENTS AND METHODS One hundred and nine patients who had undergone radical resection of primary HCC at our Hospital and had experienced intrahepatic recurrence were included in this study. Patients were categorized into the early-recurrence (ER
- Published
- 2019
22. Prognostic Significance of Lymph Node Dissection Along the Upper-third-stomach in Patients With Lower-third Gastric Cancer
- Author
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Hidenori Akaike, Makoto Sudo, Hiromichi Kawaida, Suguru Maruyama, Hiroki Shimizu, Yoshihiko Kawaguchi, Hidetake Amemiya, Shinji Furuya, Daisuke Ichikawa, Shingo Inoue, Hiroshi Kono, Kensuke Shiraishi, Ryo Saito, and Naohiro Hosomura
- Subjects
Male ,Cancer Research ,medicine.medical_specialty ,Left gastric artery ,medicine.medical_treatment ,Kaplan-Meier Estimate ,Dissection (medical) ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Gastrectomy ,Stomach Neoplasms ,medicine.artery ,medicine ,Humans ,Survival rate ,Lymph node ,Aged ,business.industry ,Stomach ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Curvatures of the stomach ,medicine.anatomical_structure ,Oncology ,Lymphatic Metastasis ,030220 oncology & carcinogenesis ,Lymph Node Excision ,Female ,Radiology ,business - Abstract
Background/aim The extent of lymph node (LN) dissection is defined according to the type of gastrectomy regardless of tumor location in recent Japanese gastric cancer treatment guidelines. However, lymphatic flow from lower-third stomach mainly drain to supra- and infra-pyloric nodes, as well as to partially lesser curvature nodes along the descending limb of the left gastric artery. In this study, we evaluated the prognostic impact of LN dissection of right paracardial (No. 1) and left greater curvature (No. 4sb) nodes in gastric cancer of lower-third stomach (LGC). Patients and methods A total of 239 patients with LGC who underwent distal gastrectomy at our hospital were retrospectively analyzed. The therapeutic value index (TVI) of each node was calculated by multiplying the incidence of LN metastasis by the 5-year survival rate of patients with metastasis to each nodal station. Results The incidence of No. 1 LN metastasis was 4.5% (positive/negative; 5/110 cases, unknown or no description; 129 cases). The 5-year survival rate of patients with metastasis to the node was 0%, and consequently the TVI of No. 1 LN station was "0". Similarly, the TVI of No. 4sb was found to be "0". Conclusion Survival benefit of dissection of No. 1 and No. 4sb LNs was presumed to be extremely low, suggesting that dissection of these two LNs could be omitted in LGC patients when undergoing distal gastrectomy.
- Published
- 2019
23. High miR-3687 Expression Affects Migratory and Invasive Ability of Oesophageal Carcinoma
- Author
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Kotaro Hagio, Jiro Nakamura, Kensuke Shiraishi, Hiroki Shimizu, Daisuke Ichikawa, Naohiro Hosomura, Hiroshi Kono, Yoshihiko Kawaguchi, Shinji Furuya, Hiromichi Kawaida, Shingo Inoue, Hidetake Amemiya, Makoto Sudo, Hidenori Akaike, and Suguru Maruyama
- Subjects
Male ,Cancer Research ,Esophageal Neoplasms ,Motility ,Biology ,Polymerase Chain Reaction ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Cell Movement ,Cell Line, Tumor ,microRNA ,Humans ,Neoplasm Invasiveness ,RNA, Small Interfering ,Basaloid Squamous Cell Carcinoma ,neoplasms ,Aged ,Cell Proliferation ,Gene knockdown ,Cell growth ,Gene Expression Profiling ,Cell Differentiation ,Cell migration ,General Medicine ,Middle Aged ,Prognosis ,digestive system diseases ,Esophagectomy ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Oncology ,Cell culture ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Cancer research ,Female - Abstract
Background/aim Basaloid squamous cell carcinoma of the oesophagus (BSCCE) is a variant of oesophageal squamous cell carcinoma (ESCC), that has a more aggressive biological behaviour than that of typical ESCC. miR-3687 has been previously identified to be highly expressed in BSCCE. This study aimed to evaluate the prognostic impact of miR-3687 in ESCC and determine the role of miR-3687 in ESCC motility. Materials and methods miR-3687 expression in human ESCC cell lines and in primary tumour samples obtained from patients with ESCC who underwent esophagectomy were analyzed via real-time polymerase chain reaction (PCR). Knockdown and over-expression experiments were conducted with miR-3687 siRNA and miRNA mimic, and the effect on cell proliferation, migration, and invasion was analyzed. Results A total of 92 samples were analyzed. High miR-3687 expression was correlated with poor prognosis. miR-3687 upregulation promoted cell migration and invasion. Conclusion miR-3687 expression negatively impacts the prognosis of patients with ESCC.
- Published
- 2019
24. Use of a Reinforced Triple-row Stapler Following Distal Pancreatectomy Reduces the Incidence of Postoperative Pancreatic Fistula in Patients With a High BMI
- Author
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Naohiro Hosomura, Ryo Saito, Hideki Fujii, Hiroshi Kono, Yoshihiko Kawaguchi, Makoto Sudo, Hiroki Shimizu, Hiromichi Kawaida, Hidenori Akaike, Mitsuaki Watanabe, Jun Itakura, Daisuke Ichikawa, Masanori Matusda, Shinji Furuya, Kazunori Takahashi, Atsushi Yamamoto, and Hidetake Amemiya
- Subjects
Male ,Cancer Research ,medicine.medical_specialty ,Body Mass Index ,Pancreatic Fistula ,03 medical and health sciences ,Pancreatectomy ,Postoperative Complications ,Surgical Staplers ,0302 clinical medicine ,Risk Factors ,Surgical Stapling ,Humans ,Medicine ,In patient ,Obesity ,Prospective Studies ,Aged ,Perioperative management ,business.industry ,Incidence ,Incidence (epidemiology) ,Significant difference ,General Medicine ,Middle Aged ,medicine.disease ,Surgery ,Oncology ,Pancreatic fistula ,030220 oncology & carcinogenesis ,Female ,business ,Distal pancreatectomy ,Pancreatic stump ,Body mass index - Abstract
Background/aim Although perioperative management and operative techniques for pancreatic surgery have improved, postoperative pancreatic fistula (POPF) remains the major cause of morbidity and mortality following distal pancreatectomy (DP). The purpose of this study was to evaluate the superiority of the reinforced stapler compared to the bare triple row stapler. Patients and methods A total of 93 patients who underwent DP at the First Department of Surgery at Yamanashi University were examined. The patients were divided into two groups according to the closure method for the pancreatic stump; the bare triple-row stapler (BTRS) group and the reinforced triple-row stapler (RTRS) group. The postoperative outcomes were then compared in terms of several clinicopathological factors between the two groups. Results Seven patients were diagnosed with Grade B/C POPF in this series. The incidence of POPF in the RTRS group was lower than that in the BTRS group (3.6% vs. 13.5%), although there was no significant difference (p=0.077). Further detailed analysis demonstrated that RTRS significantly reduced POPF compared to BTRS in obese patients with a BMI >25.0 kg/m2 (p=0.038). Conclusion Reinforced triple-row staplers may reduce the incidence of severe POPF, especially in obese patients with a BMI >25 kg/m2.
- Published
- 2019
25. A 27×18 cm Solitary Fibrous Tumor Derived from the Mesorectum Resected by Transsacral followed by Abdominal Approach —A Case Report
- Author
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Takashi Nakayama, Hiroshi Iino, Kunio Mochizuki, Ryohei Kato, Yoshihiro Akazawa, Michio Hara, Makoto Sudo, and Daisuke Ichikawa
- Subjects
medicine.medical_specialty ,business.industry ,Family medicine ,Medicine ,business - Published
- 2019
26. Feasibility of Combination Therapy with Nab-paclitaxel Plus Gemcitabine in Patients with Recurrent Pancreatic Cancer
- Author
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Hiroki Shimizu, Naohiro Hosomura, Jun Itakura, Makoto Sudo, Daisuke Ichikawa, Hideki Fujii, Hidenori Akaike, Shinji Furuya, Shintaro Ichikawa, Yoshihiko Kawaguchi, Hiromichi Kawaida, Hiroshi Kono, Mitsuharu Fukasawa, Erika Takahashi, Mitsuaki Watanabe, Ryou Saitou, Hiroshi Hayakawa, Ayako Kimura, Shinichi Takano, Hiroko Shindo, Hidetake Amemiya, and Atsushi Yamamoto
- Subjects
Male ,0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,Paclitaxel ,Combination therapy ,medicine.medical_treatment ,Neutropenia ,Deoxycytidine ,Disease-Free Survival ,03 medical and health sciences ,0302 clinical medicine ,Albumins ,Pancreatic cancer ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Medicine ,In patient ,Aged ,Nab-paclitaxel ,business.industry ,General Medicine ,Middle Aged ,medicine.disease ,Gemcitabine ,Pancreatic Neoplasms ,Regimen ,Treatment Outcome ,030104 developmental biology ,030220 oncology & carcinogenesis ,Pancreatectomy ,Feasibility Studies ,Administration, Intravenous ,Female ,Neoplasm Recurrence, Local ,business ,medicine.drug - Abstract
Background/aim Nab-paclitaxel plus gemcitabine (nab-P+Gem) is one of most reliable and effective regimens for borderline or unresectable pancreatic cancer (PC). However, the feasibility and clinical benefits of this regimen have never been evaluated for patients with recurrent PC after pancreatectomy. The aim of this study was to investigate the feasibility of combination therapy with nab-paclitaxel plus gemcitabine (nab-P+Gem) for patients with recurrent PC. Patients and methods Twenty-two patients with recurrent PC received an intravenous infusion of nab-P (125 mg/m2) and Gem (1,000 mg/m2) on days 1, 8, and 15 of a 4-week cycle. The primary end-point of this study was completion of the 4 cycles. The secondary end-points were the safety, efficacy, and disease control rate. Results The treatment completion rate of the 4 cycles was 90.9%. The objective response rate was 13.6% and the disease control rate was 63.6%. The median progression-free survival was 7.2 months. The most common grade 3 or higher hematological toxicity was neutropenia (72.7%). There was no treatment-related death. Furthermore, the chemotherapeutic effects varied with the time of recurrence. Conclusion Combination nab-P+Gem therapy was well-tolerated and effective in patients with recurrent PC.
- Published
- 2018
27. A case of simultaneous laparoscopic surgery for double cancer comprising multiple early gastric cancer and advanced sigmoid colon cancer after revascularization
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Yoshihiko Kawaguchi, Katsutoshi Shoda, Naohiro Hosomura, Hiroshi Kono, Atsushi Yamamoto, Daisuke Ichikawa, Ryo Saito, Kazuyoshi Hirayama, Shinji Furuya, Hidetake Amemiya, Hidenori Akaike, Hiromichi Kawaida, Makoto Sudo, and Koichi Takiguchi
- Subjects
Laparoscopic surgery ,medicine.medical_specialty ,medicine.medical_treatment ,lcsh:Surgery ,Case Report ,Double cancer ,Anastomosis ,Revascularization ,Inferior mesenteric artery ,03 medical and health sciences ,0302 clinical medicine ,medicine.artery ,medicine ,business.industry ,lcsh:RD1-811 ,Blood flow ,Common iliac artery ,Internal iliac artery ,Surgery ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,After revascularization ,030211 gastroenterology & hepatology ,business ,Blood vessel - Abstract
Background Traditionally, the surgery for simultaneous double cancer of the stomach and colon required a large incision to the upper and lower region of the abdomen. In this case, an artificial blood vessel was located under the skin after revascularization. Considering ischemia due to graft compression by incision retractor during laparotomy, this was difficult to do. This is a report on laparoscopic surgery for simultaneous double cancer of the stomach and colon after revascularization. Case presentation A 69-year-old man had early gastric cancer and advanced sigmoid colon cancer. He had suffered from thromboangitis obliterans and has undergone revascularization many times due to poor blood flow in his lower limbs. He had had some artificial blood vessels inserted under the skin, confirmed by blood vessel construction image by preoperative computed tomography (CT). There was a bypass vessel from the left axillary artery to the left femoral artery under the skin of the left thoracoabdominal. In addition, there were two bypass vessels from the left external iliac artery to the right femoral artery under the skin of the lower abdomen. One of the two bypasses was occluded. In the blood flow to the intestinal tract, the inferior mesenteric artery was already occluded. Peripheral blood flow in the common iliac artery depended on blood flow from the artificial blood vessel, and blood flow from the internal iliac artery to the rectum was poor. Laparoscopic Hartmann’s operation was performed for Stage II B (UICC 8th Edition) sigmoid colon cancer. Because the blood flow in the intestinal tract on the anal side was poor, we thought that anastomosis was at a high risk for leakage. Laparoscopic total gastrectomy was also performed simultaneously for two Stage I (UICC 8th edition) gastric cancers in the cardia and body. The location of the port site and stoma was carefully determined preoperatively to prevent damage and infection to the artificial blood vessels. Minimal invasive surgery was performed using laparoscopic surgery. Conclusions Laparoscopic surgery with small incisions is useful for patients with double cancer who need an approach to the upper and lower abdomen. Furthermore, laparoscopic surgery has less interference on graft in patients with artificial blood vessels under the skin by intraperitoneal approach.
- Published
- 2021
28. Exposure to Blood Components and Inflammation Contribute to Pancreatic Cancer Progression
- Author
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Atsushi Yamamoto, Shingo Inoue, Hidenori Akaike, Koichi Takiguchi, Hiroshi Kono, Shinji Furuya, Suguru Maruyama, Yoshihiko Kawaguchi, Hiromichi Kawaida, Katsutoshi Shoda, Ryo Saito, Naohiro Hosomura, Makoto Sudo, Hidetake Amemiya, Jun Itakura, and Daisuke Ichikawa
- Subjects
medicine.medical_specialty ,medicine.medical_treatment ,Inflammation ,030230 surgery ,Gastroenterology ,03 medical and health sciences ,Pancreatic Fistula ,0302 clinical medicine ,Pancreatectomy ,Postoperative Complications ,Surgical oncology ,Risk Factors ,Internal medicine ,Pancreatic cancer ,medicine ,Overall survival ,Humans ,Platelet ,Retrospective Studies ,business.industry ,medicine.disease ,In vitro ,Pancreatic Neoplasms ,Oncology ,Pancreatic fistula ,030220 oncology & carcinogenesis ,Surgery ,medicine.symptom ,business - Abstract
Pancreatectomy is a highly invasive procedure with extensive intraoperative blood loss (IBL) and high risk of postoperative pancreatic fistula (POPF). We conducted an experimental and retrospective clinical study to determine whether the malignant behaviors of pancreatic cancer cells were enhanced by exposure to blood components in vitro and to evaluate the oncological significance of high IBL and POPF in pancreatic cancer. This study included 107 patients undergoing radical pancreatectomy in the University of Yamanashi Hospital between 2011 and 2017, classified into high (n = 29) and low (n = 78) IBL groups. In vitro experiments included functional analyses of Panc-1 pancreatic cancer and normal mesothelial cells exposed to patient blood components, and clinical data were used to assess the contribution of IBL and POPF to patient outcomes. The migration (p = 0.007), invasion (p < 0.001), and proliferation (p < 0.01) of Panc-1 cells were enhanced with platelet coculture. The ability of Panc-1 cells to adhere mesothelial cells was enhanced by plasma coincubation, especially in the presence of inflammation (p < 0.001). High IBL was associated with worse overall survival (p = 0.007) and increased locoregional recurrence (p = 0.003) in patients. POPF enhanced the negative prognostic significance of high IBL (p < 0.001 for overall survival, p = 0.001 for locoregional recurrence), indicating the oncological negative effects of high IBL and POPF. Blood components, especially platelets, and inflammation enhance the malignant behaviors of pancreatic cancer cells, potentially contributing to poor prognosis for pancreatic cancer patients.
- Published
- 2021
29. Recombinant Human Thrombomodulin Has Additive Effects in Septic Patients Undergoing Continuous Hemodiafiltration Due to Intestinal Perforation
- Author
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Hidetake Amemiya, Hiroshi Kono, Daisuke Ichikawa, Hidenori Akaike, Shinji Furuya, Makoto Sudo, Naohiro Hosomura, Hiromichi Kawaida, and Yoshihiko Kawaguchi
- Subjects
Male ,medicine.medical_specialty ,Thrombomodulin ,Perforation (oil well) ,Acute Lung Injury ,Hemodiafiltration ,Lung injury ,Critical Care and Intensive Care Medicine ,Gastroenterology ,Sepsis ,Cohort Studies ,Internal medicine ,medicine ,Animals ,Humans ,Platelet ,Aged ,Disseminated intravascular coagulation ,Aged, 80 and over ,APACHE II ,business.industry ,Middle Aged ,medicine.disease ,Recombinant Proteins ,Rats ,Disease Models, Animal ,Intestinal Perforation ,Emergency Medicine ,Tumor necrosis factor alpha ,Female ,business - Abstract
Background Disseminated intravascular coagulation (DIC) is associated with high mortality in patients with severe sepsis. The purpose of this study was to investigate the effects of recombinant human thrombomodulin (rhTM) in septic patients undergoing continuous hemodiafiltration (CHDF). Furthermore, effects of rhTM in acute lung injury, the first target organ in sepsis, was investigated using a sepsis model in rats. Methods Clinical laboratory data, and the DIC, Sequential Organ Failure Assessment (SOFA), and Acute Physiologic and Chronic Health Evaluation (APACHE) II scores were compared between patients undergoing CHDF alone (rhTM- group; n = 23 cases) and patients undergoing CHDF treated with rhTM (rhTM+ group; n = 21 cases). Rats underwent cecal ligation and puncture (CLP) treated with or without rhTM, and acute lung injury (ALI) was analyzed. Production of TNF-α by isolated tissue macrophages (Mfs) was assessed. Results The numbers of leukocytes, and C-reactive protein and D-dimer levels were significantly suppressed, and platelet counts recovered significantly faster in the rhTM+ group compared with the rhTM- group. The DIC score was reduced in both groups. The SOFA and APACHE II scores gradually reduced in the rhTM+ group. The CHDF treatment and ICU admission periods were shortened in the rhTM+ group compared with the rhTM- group. Treatment of rhTM was an independent factor for CHDF treatment period by multivariate analyses. CLP-induced ALI was significantly improved by rhTM. Increased TNF-α production by tissue Mfs was significantly suppressed by rhTM in vivo and in vitro. Conclusion Additive effects of rhTM treatment were observed in septic patients undergoing CHDF.
- Published
- 2021
30. Prognostic impact of lymph node micrometastasis in patients with gastric cancer
- Author
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Yoshihiko Kawaguchi, Atsushi Yamamoto, Makoto Sudo, Naoki Ashizawa, Daisuke Ichikawa, Hiroshi Kono, Hidenori Akaike, Shinji Furuya, Hiromichi Kawaida, Hidetake Amemiya, Kunio Mochizuki, Ryo Saito, Koichi Takiguchi, Katsutoshi Shoda, and Naohiro Hosomura
- Subjects
Adult ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,030230 surgery ,Gastroenterology ,03 medical and health sciences ,0302 clinical medicine ,Surgical oncology ,Stomach Neoplasms ,Internal medicine ,medicine ,Biomarkers, Tumor ,Humans ,Clinical significance ,Lymph node ,Aged ,Neoplasm Staging ,Retrospective Studies ,Aged, 80 and over ,business.industry ,Proportional hazards model ,Micrometastasis ,Cancer ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,medicine.anatomical_structure ,Neoplasm Micrometastasis ,030220 oncology & carcinogenesis ,Lymphatic Metastasis ,Surgery ,Gastrectomy ,Female ,Lymph ,Lymph Nodes ,business - Abstract
The clinical significance of lymph node micrometastasis (LNMM) remains controversial in gastric cancer (GC). In this study, we investigated the prognostic impact of LNMM in patients with GC. A total of 624 patients with pathologically lymph node metastasis-negative (pN0) and N1 status (pN1) who underwent gastrectomy between 2004 and 2018 were enrolled in this retrospective study. The diameter of tumor cell clusters in metastatic lymph nodes was measured in 120 patients with pN1 GC. Patients with lymph node tumors
- Published
- 2021
31. [Nab-Paclitaxel plus Gemcitabine for Patients with Recurrence after Resection for Adenocarcinoma of the Pancreas]
- Author
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Hiromichi, Kawaida, Naohiro, Hosomura, Hidetake, Amemiya, Yudai, Higuchi, Isamu, Tsukahara, Takashi, Nakayama, Ryo, Saito, Katsutoshi, Shoda, Yuuki, Nakata, Shinji, Furuya, Hidenori, Akaike, Yoshihiko, Kawaguchi, Makoto, Sudo, Hiroshi, Kono, and Daisuke, Ichikawa
- Subjects
Pancreatic Neoplasms ,Paclitaxel ,Albumins ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Adenocarcinoma ,Neoplasm Recurrence, Local ,Deoxycytidine ,Pancreas ,Gemcitabine - Abstract
The purpose of this study was to clarify the predictors of efficacy of nab-paclitaxel plus gemcitabine(GnP)for patients with recurrence after resection for adenocarcinoma of the pancreas.Patients who had received GnP at our hospital were divided into 2 groups; effective group and non-effective group and we compared them. In addition, we compared the therapeutic effect of patients between well-differentiated adenocarcinoma and moderately differentiated adenocarcinoma. Furthermore, we compared the efficacy depending on the time of recurrence.In patients with well-differentiated adenocarcinoma, the disease control rate was 93.6% and progression free survival was 8.6 months, whereas those in patients with moderately differentiated adenocarcinoma were 57.1% and 4.4 months, respectively. Patients who recurred at 7 months or later, had a better therapeutic response than the patients who recurred within 6 months after surgery.GnP may be effective in patients with well-differentiated adenocarcinoma and in patients who recurred at 7 months or later.
- Published
- 2021
32. [Pathological Complete Response of Intrapelvic Recurrence of Rectal Cancer after Chemoradiotherapy-A Case Report]
- Author
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Atsushi, Yamamoto, Shinji, Furuya, Koichi, Takiguchi, Kazuyoshi, Hirayama, Michio, Hara, Makoto, Sudo, Naoki, Ashizawa, Katsutoshi, Shoda, Hidenori, Akaike, Naohiro, Hosomura, Yoshihiko, Kawaguchi, Hidetake, Amemiya, Hiromichi, Kawaida, Hiroshi, Kono, and Daisuke, Ichikawa
- Subjects
Male ,Rectal Neoplasms ,Humans ,Lymph Node Excision ,Chemoradiotherapy ,Neoplasm Recurrence, Local ,Neoplasm Staging ,Pelvis - Abstract
50's man who have performed anterior pelvic exenteration with lateral lymph node dissection for rectal cancer with pT4bN0M0, pStage Ⅱc about 2 years ago, was admitted to our hospital for the treatment of intrapelvic recurrence of rectal cancer. No distant metastasis was found in the computed tomography examination but the tumor invaded the dorsal side of the pubis. Because radical excision was impossible with these findings, he received chemoradiotherapy(CRT). Post-CRT imaging showed that the tumor of intrapelvic recurrence region reduced the size, and invasion of pubis had disappeared and been markedly reduced. Thus, radical excision seemed possible at this point, and we decided to attempt operation after total 6 weeks of S-1(120 mg/day)regimen and radiation(40 Gy/20 Fr). We performed Miles' operation. The final pathological examination demonstrated that no viable tumor cells remained in the resected rectum specimen, confirming that a pathological complete response(pCR)had been achieved.
- Published
- 2021
33. Stroke Volume Variation Monitoring to Minimize Blood Loss in Hepatocellular Carcinoma Resection
- Author
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Hidenori Akaike, Takashi Nakayama, Katsutoshi Shoda, Hidetake Amemiya, Naohiro Hosomura, Daisuke Ichikawa, Yoshihiko Kawaguchi, Makoto Sudo, Shinji Furuya, Yudai Higuchi, Hiromichi Kawaida, Shingo Inoue, Ryo Saito, and Hiroshi Kono
- Subjects
Male ,Cancer Research ,medicine.medical_specialty ,Blood transfusion ,Carcinoma, Hepatocellular ,medicine.medical_treatment ,Blood Loss, Surgical ,Resection ,Postoperative Complications ,Blood loss ,Monitoring, Intraoperative ,medicine ,Hepatectomy ,Humans ,Blood Transfusion ,Aged ,Retrospective Studies ,business.industry ,Incidence (epidemiology) ,Liver Neoplasms ,Stroke Volume ,General Medicine ,Perioperative ,Stroke volume ,Middle Aged ,medicine.disease ,Surgery ,Oncology ,Hepatocellular carcinoma ,Heart Function Tests ,Female ,business ,Biomarkers - Abstract
BACKGROUND/AIM We aimed to evaluate the correlation between stroke volume variation (SVV) and intraoperative blood loss (IBL) in hepatocellular carcinoma (HCC) resection and examine the perioperative utility of SVV-based management. PATIENTS AND METHODS Ninety-five patients who underwent partial or sub-segmental hepatectomy for HCC between 2013 and 2019 at the University of Yamanashi Hospital were retrospectively analyzed. A correlation analysis between IBL and SVV was performed, and then all cases were divided into three groups: high, middle, and low-SVV groups. Perioperative short-term outcomes based on SVV groups were analyzed. RESULTS There was a weak but significant negative correlation between SVV and IBL (ρ=-0.372, p
- Published
- 2020
34. ASO Visual Abstract: Exposure to Blood Components and Inflammation Contribute to Pancreatic Cancer Progression
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Atsushi Yamamoto, Suguru Maruyama, Yoshihiko Kawaguchi, Makoto Sudo, Hidenori Akaike, Jun Itakura, Daisuke Ichikawa, Hidetake Amemiya, Katsutoshi Shoda, Shinji Furuya, Naohiro Hosomura, Hiroshi Kono, Shingo Inoue, Ryo Saito, Koichi Takiguchi, and Hiromichi Kawaida
- Subjects
Oncology ,medicine.medical_specialty ,business.industry ,Surgical oncology ,Pancreatic cancer ,Internal medicine ,medicine ,Surgery ,Inflammation ,medicine.symptom ,medicine.disease ,business - Published
- 2021
35. Cytoglobin-expressing cells in the splenic cords contribute to splenic fibrosis in cirrhotic patients
- Author
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Yuji, Iimuro, Akito, Yada, Toshihiro, Okada, Ikuo, Nakamura, Kazuhiro, Suzumura, Jinyang, Xu, Makoto, Sudo, Shuhei, Nishiguchi, Norifumi, Kawada, Etsuro, Hatano, and Jiro, Fujimoto
- Subjects
Liver Cirrhosis ,Male ,Cytoglobin ,Middle Aged ,Oxidative Stress ,Hypertension, Portal ,Disease Progression ,Splenectomy ,Humans ,Female ,Laparoscopy ,Biomarkers ,Spleen ,Aged ,Splenic Diseases - Abstract
Among several noninvasive evaluation methods of portal hypertension (PH), the measurement of spleen stiffness is a reliable method for predicting esophageal variceal bleeding; however, the underlying mechanisms for increased stiffness remain unclear. We attempted to elucidate the pathological changes to the spleen and the underlying mechanisms in patients with PH.Histological examination was performed using splenic tissues from 42 patients with PH who underwent laparoscopic splenectomy, and the results were compared with those from patients without PH.In addition to splenic sinus congestion, diffuse fibrosis was detected in the splenic cords in the red pulp of patients with PH. The degree of the fibrosis was well correlated with severity in thrombocytopenia and splenomegaly. Cells expressing α-smooth muscle actin dramatically increased in the splenic cord. Cytoglobin (Cygb) expression was detected in human splenic cords as reported in animal reticular cells, and fluorescent double immunostaining revealed that these cells expressed α-smooth muscle actin in patients with PH, suggesting transformation of Cygb-expressing cells to myofibroblastic cells. Expression levels of nicotinamide adenine dinucleotide phosphate oxidase (NOX) 2, nitrotyrosine, and transforming growth factor-β were markedly upregulated in the red pulp of patients with PH, implying a significant role of oxidative stress in the mechanism for splenic fibrosis.Splenic fibrosis progresses along with advancement of PH. Cygb-expressing cells in the splenic cord possibly participate in this process through mechanisms including oxidative stress.
- Published
- 2020
36. 82 POSSIBLE NOVEL BIOMARKER OF ESOPHAGEAL SQUAMOUS CELL CARCINOMA
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K Takiguchi, Hidenori Akaike, Suguru Maruyama, Hiromichi Kawaida, Hiroshi Kono, Daisuke Ichikawa, Shinji Furuya, Naohiro Hosomura, Yoshihiko Kawaguchi, Hidetake Amemiya, R. Saito, Makoto Sudo, Katsutoshi Shoda, and A Yamamoto
- Subjects
business.industry ,Gastroenterology ,Cancer research ,Medicine ,Biomarker (medicine) ,General Medicine ,business ,Esophageal squamous cell carcinoma - Abstract
Recently, patients with esophageal squamous cell carcinoma (ESCC) have been treated with combination therapy, including surgical resection, chemotherapy, and/or radiation therapy. Therefore, the development of novel and useful biomarkers is expected because the malignant behavior of tumors and the treatment effect vary in each case. In this study, we examined the clinical significance of microRNA-X (miR-X) as a biomarker because it has been reported in studies that examined ESCC cell-lines in vitro. Methods In all, 81 consecutive patients who underwent radical esophagectomy without preoperative treatment for ESCC were examined. Total RNA was extracted from formalin-fixed paraffin-embedded samples and miR-X expression levels were evaluated. In this process, the miR-X levels were standardized against the RNU6B expression levels and were analyzed using the ΔΔCq method. Clinicopathological features between high and low expression groups of miR-X were compared using the median expression level as the cutoff value. Prognostic analyses were performed using several factors, including miR-X expression levels, to gage the cancer-specific (CSS) and recurrence-free survival (RFS) of these patients. Results High miR-X expression was found to be significantly related to deep invasion (p Conclusion The present study revealed that high miR-X expression was related to deep invasion and high vascular invasion in ESCC. It was also independent adverse prognostic factor for patients with ESCC. Detailed examination including in vitro re-verification is required because these results were inconsistent with previous reports and miR-X might play different roles in clinical settings. In any case, further detailed studies might establish the significance of miR-X as a novel and useful biomarker of ESCC.
- Published
- 2020
37. Anastomosis Technique For Pancreatojejunostomy And Early Removal Of Drainage Tubes May Reduce Postoperative Pancreatic Fistula
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Masanori Matusda, Makoto Sudo, Hiroki Shimizu, Katsutoshi Shoda, Hidenori Akaike, Naohiro Hosomura, Ryo Saito, Mitsuaki Watanabe, Hideki Fujii, Yuuki Nakata, Hidetake Amemiya, Hiroshi Kono, Shinji Furuya, Yoshihiko Kawaguchi, Jun Itakura, Daisuke Ichikawa, and Hiromichi Kawaida
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medicine.medical_specialty ,medicine.medical_treatment ,lcsh:Surgery ,Amylase levels ,Modified method ,030230 surgery ,Anastomosis ,lcsh:RC254-282 ,Pancreaticoduodenectomy ,03 medical and health sciences ,Pancreatic Fistula ,0302 clinical medicine ,Postoperative Complications ,Drainage tubes ,Risk Factors ,Pancreaticojejunostomy ,medicine ,Humans ,Pancreas ,Retrospective Studies ,business.industry ,Research ,Anastomosis, Surgical ,lcsh:RD1-811 ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Prognosis ,Surgery ,medicine.anatomical_structure ,Oncology ,Pancreatic fistula ,030220 oncology & carcinogenesis ,Amylases ,Drainage ,business - Abstract
Background Postoperative pancreatic fistula (POPF) is one of the most serious complications after pancreaticoduodenectomy (PD). Various factors have been reported as POPF risks, but the most serious of these is soft pancreas. To reduce POPF occurrences, many changes to the PD process have been proposed. This study evaluates short-term results of anastomosis technique for PD. Methods In total, 123 patients with soft pancreases who had undergone PD at Yamanashi University between January 2012 and August 2020 were retrospectively analyzed. We divided these patients into two groups depending on the time PD was performed: a conventional group (n = 67) and a modified group (n = 56). Results The rate of clinically relevant POPF was significantly lower in the modified group than that in the conventional group (5.4% vs 22.4%, p value p value = 0.002). Conclusions Our novel anastomosis technique for pancreatojejunostomy reduced POPF in PD, especially in cases where the patient had a soft pancreas.
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- 2020
38. Lithium promotes malignant transformation of nontumorigenic cells in vitro
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Makoto Sudo, Masafumi Yoshinaga, Kazunori Hashimoto, Lisa Kondo-Ida, Mohammad Daud Azimi, Kiyoshi Yanagisawa, Nobuyuki Hamajima, Masashi Kato, and Said Hafizullah Fayaz
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MAPK/ERK pathway ,Keratinocytes ,Environmental Engineering ,010504 meteorology & atmospheric sciences ,Lithium (medication) ,010501 environmental sciences ,Lithium ,medicine.disease_cause ,01 natural sciences ,Malignant transformation ,Cell Line ,medicine ,Environmental Chemistry ,Humans ,Viability assay ,Waste Management and Disposal ,Protein kinase B ,0105 earth and related environmental sciences ,Chemistry ,Afghanistan ,Pollution ,In vitro ,HaCaT ,Cell Transformation, Neoplastic ,Cancer research ,Carcinogenesis ,medicine.drug - Abstract
Because of the deficiency of water caused by the regional disparities of rainfall due to global warming, attention has been given to the use of well water as drinking water in developing countries. Our fieldwork study in Afghanistan showed that there was a maximum value of 3371 μg/L and an average value of 233 μg/L of lithium in well drinking water. Since the level of lithium in well water is higher than the levels in other countries, we investigated the health risk of lithium. After confirming no influence of ≤1000 μM lithium on cell viability, we found that lithium at concentrations of 100 and 500 μM promoted anchorage-independent growth of human immortalized keratinocytes (HaCaT) and lung epithelial cells (BEAS-2B) but not that of human keratinocytic carcinoma cells (HSC-5) or lung epithelial carcinoma cells (A549). The same concentrations of lithium also promoted phosphorylation of c-SRC and MEK/ERK but not that of AKT in the keratinocytes. Inhibitors of c-SRC (PP2) and MEK (PD98059) suppressed the lithium-induced increase in anchorage-independent growth of the keratinocytes. Our results suggested that lithium promoted transformation of nontumorigenic cells rather than progression of tumorigenic cells with preferential activation of the c-SRC/MEK/ERK pathway. Since previous pharmacokinetics studies indicated that it is possible for the serum level of lithium to reach 100 μM by drinking 2.5 L of water containing 3371 μg/L of lithium per day, the high level of lithium contamination in well drinking water in Kabul might be a potential oncogenic risk in humans.
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- 2020
39. [Effects of Preoperative Immune and Nutritional Indices on the Prognosis of pStage Ⅱ and Ⅲ Colorectal Cancer]
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Yuuki, Nakata, Shinji, Furuya, Kouichi, Takiguchi, Naoki, Ashizawa, Hiroki, Shimizu, Hidenori, Akaike, Naohiro, Hosomura, Yoshihiko, Kawaguchi, Hidetake, Amemiya, Makoto, Sudo, Hiromichi, Kawaida, Shingo, Inoue, Hiroshi, Kono, and Daisuke, Ichikawa
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Nutrition Assessment ,Humans ,Lymphocytes ,Colorectal Neoplasms ,Prognosis ,Neoplasm Staging ,Retrospective Studies - Abstract
We examined the relationship between immune and nutritional indices and the outcome of colorectal cancer patients at our hospital. We retrospectively analyzed 196 patients with pStage Ⅱand Ⅲcolorectal cancer who underwent curative surgery in our institution between 2007 and 2013. The evaluation items were immune and nutritional indices, such as neutrophil/lym- phocyte ratio(NLR), platelet/lymphocyte ratio(PLR), lymphocyte/monocyte ratio(LMR), and the Onodera nutritional index (PNI). Moreover, we analyzed the relationship between immune and nutritional indices and outcome, overall survival(OS), and recurrence-free survival(RFS). Univariate and multivariate analyses showed that low LMR was significantly related to high mortality. Univariate analysis showed that high NLR, low LMR, and low PNIwere significantly related to a decrease in OS. Multivariate analysis showed that PNIwas an independent predictor of OS. LMR is suggested as a new predictor for postoperative complications. PNIis suggested as a new prognostic factor in pStageⅡand Ⅲcolorectal cancer.
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- 2020
40. [Efficacy of Surgery and Adjuvant Chemotherapy for Distal Cholangiocarcinoma]
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Kazunori, Takahashi, Hiromichi, Kawaida, Naohiro, Hosomura, Hidetake, Amemiya, Hiroshi, Kono, Yuuki, Nakata, Hiroki, Shimizu, Kensuke, Shiraishi, Shinji, Furuya, Hidenori, Akaike, Yoshihiko, Kawaguchi, Makoto, Sudo, Shingo, Inoue, and Daisuke, Ichikawa
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Cholangiocarcinoma ,Bile Ducts, Intrahepatic ,Bile Duct Neoplasms ,Chemotherapy, Adjuvant ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Disease-Free Survival ,Retrospective Studies - Abstract
Surgery alone shows an insufficient result for distal cholangiocarcinoma, and postoperative adjuvant chemotherapy is commonly used. However, no definite opinion has yet been accepted.A group of 46 patients who underwent surgery for distal bile duct cancer and who received adjuvant chemotherapy including gemcitabine (GEM)(Group A)and surgery alone group(Group S)were compared for disease-free survival(DFS)and overall survival (OS).Although the median DFS was 718 days in Group A and 367 days in Group S(p=0.306)and the median OS was 1,171 days in Group A and 859 days in Group S(p=0.07), no significant difference was observed; however, the prognosis improved.Postoperative adjuvant chemotherapy may improve prognosis.
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- 2020
41. [Pathologic Complete Response of Advanced Gastric Cancer after Chemotherapy with S-1 and Oxaliplatin and Conversion Surgery-A Case Report]
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Atsushi, Yamamoto, Hidenori, Akaike, Yoshihiko, Kawaguchi, Kazunori, Takahashi, Suguru, Maruyama, Yuuki, Nakata, Hiroki, Shimizu, Shinji, Furuya, Naohiro, Hosomura, Hidetake, Amemiya, Hiromichi, Kawaida, Makoto, Sudo, Hiroshi, Kono, Shingo, Inoue, and Daisuke, Ichikawa
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Male ,Oxaliplatin ,Gastrectomy ,Stomach Neoplasms ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Lymph Node Excision ,Aged - Abstract
A man in his 70s was admitted to our hospital for the treatment of gastric cancer type 3 located in the antrum of the stomach. Computed tomography revealed tumor invasion of the liver and metastatic lymph node invasion of the pancreatic head and splenic artery. The patient was diagnosed with unresectable T4bN3M0, Stage ⅢC advanced gastric cancer. As radical excision was impossible, the patient underwent chemotherapy with S-1 and oxaliplatin(SOX). After 13 courses of SOX, imaging showed reduction in the size of the primary tumor and disappearance or marked reduction in the size of the metastatic lymph nodes. Therefore, conversion surgery was attempted after 14 courses of SOX. Distal gastrectomy with D2 lymphadenectomy including station 14v was performed. Pathological examination demonstrated no viable tumor cells in the resected stomach specimen or dissected lymph nodes, confirming that a pathologic complete response(pCR)had been achieved.
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- 2020
42. Indications for extrahepatic bile duct resection due to perineural invasion in patients with gallbladder cancer
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Hiromichi Kawaida, Shingo Inoue, Yoshihiko Kawaguchi, Hidenori Akaike, Suguru Maruyama, Ryo Saito, Naohiro Hosomura, Daisuke Ichikawa, Hiroshi Kono, Makoto Sudo, Shinji Furuya, Hiroki Shimizu, and Hidetake Amemiya
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Male ,medicine.medical_specialty ,Lymphovascular invasion ,medicine.medical_treatment ,lcsh:Surgery ,Perineural invasion ,lcsh:RC254-282 ,Gastroenterology ,Bile Ducts, Extrahepatic ,Surgical oncology ,Internal medicine ,medicine ,Hepatectomy ,Humans ,Cholecystectomy ,Neoplasm Invasiveness ,Peripheral Nerves ,Stage (cooking) ,Gallbladder cancer ,Aged ,Retrospective Studies ,Extrahepatic bile duct resection ,Bile duct ,business.industry ,Research ,Cancer ,lcsh:RD1-811 ,Prognosis ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Survival Rate ,medicine.anatomical_structure ,Oncology ,Female ,Gallbladder Neoplasms ,Surgery ,Neoplasm Recurrence, Local ,business ,Follow-Up Studies - Abstract
BackgroundThe indications for extrahepatic bile duct (EHBD) resection remain a major controversy in the surgical management of patients with gallbladder cancer. On the other hand, perineural invasion (PNI) was reported as an important factor in patients with gallbladder cancer because gallbladder cancer cells frequently spread to the tissues surrounding the EHBD via perineural routes. We assessed the correlation of PNI with clinicopathological factors in patients with gallbladder cancer to elucidate EHBD resection indications specifically in patients with PNI.MethodsThis retrospective study assessed the PNI status of 50 patients with gallbladder cancer who underwent curative resection and examined the correlation between the presence of PNI and clinicopathological factors.ResultsThirteen patients (26%) were PNI positive. PNI was significantly correlated with male sex, proximal-type tumor, lymphatic and vascular invasion, and advanced T stage. Multivariate analysis found that PNI positivity (p< 0.001), lymphatic invasion (p= 0.007), and nodal stage (p< 0.001) were independent prognostic factors. PNI was never observed in patients with stage T1 cancer. Conversely, PNI was detected rarely in distal-type tumors, all of whom developed various types of recurrences.ConclusionsThese results clearly demonstrated the prognostic impact of PNI in patients with gallbladder cancer. We suggest that EHBD resection in combination with cholecystectomy may not be useful for distal-type tumors from a perspective of PNI.
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- 2019
43. Successful laparoscopic partial gastrectomy and spleen-preserving distal pancreatectomy for gastric duplication cyst connecting with the pancreatic tail
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Hiromichi Kawaida, Hiroshi Kono, Naohiro Hosomura, Hidenori Akaike, Hidetake Amemiya, Ayako Kimura, Mitsuaki Watanabe, Ei Takahashi, Tomohiro Inoue, Makoto Sudo, Masanori Matsuda, Hideki Fujii, Katsuhiro Sano, Yoshihiko Kawaguchi, Mitsuharu Fukasawa, and Daisuke Ichikawa
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Laparoscopic surgery ,medicine.medical_specialty ,Gastric duplication cyst ,medicine.medical_treatment ,Article ,Gastrointestinal duplication ,03 medical and health sciences ,0302 clinical medicine ,Case report ,Medicine ,Cyst ,Pancreas ,Gastric duplication ,business.industry ,Stomach ,medicine.disease ,Curvatures of the stomach ,Surgery ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,Gastrectomy ,business - Abstract
Highlights • Gastric duplication cyst(GDC) contiguous with the stomach and pancreatic tail is extremely rare. • GDCs are usually diagnosed at a younger age. In adults, they are very rare disease and the diagnosis may be difficult. • Surgical resection is considered to be the best treatment due to the difficulty of diagnosis. • Laparoscopic surgery is less invasive and should be selected whenever possible., Introduction Gastrointestinal duplication cyst is a congenital rare disease that may occur in any region from mouth to anus. Among them, gastric duplication cysts are very rare. Case report Here we report A 23-year-old Japanese man who visited our hospital to evaluate an abdominal tumor. Abdominal computed tomography showed a well-circumscribed homogenous low-density mass measuring 6.2 × 6.0 cm between the pancreatic tail and the upper posterior wall on the gastric greater curvature, and the mass seemed to originate from the pancreatic tail. We found intraoperatively that the mass adhered to the stomach and pancreatic tail strongly, so we performed laparoscopic partial gastrectomy and spleen-preserving distal pancreatectomy. Pathological findings showed that the lining epithelium of the cystic mass consisted of the gastric foveolar epithelium with fundic glands. Furthermore, the pancreatic tissue of the pancreatic tail and the muscular layer of the cystic mass were intermingled. Discussion GDCs are usually diagnosed at a younger age and in adults, they are very rare. Therefore, surgical resection is considered to be the best treatment due to the difficulty of diagnosis, and also that it mimics a pancreatic cystic tumor, and malignant transformation. Complete resection of the cyst is the ideal technique and laparoscopic surgery should be selected whenever possible. Conclusion We experienced a case of GDC continuous to both stomach and pancreatic tail. Laparoscopic surgery is safety and useful even if GDC is continuous with both the stomach and the pancreas.
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- 2018
44. Prognostic effect of sarcopenia in colorectal cancer recurrence
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Hiromichi Kawaida, Yoshihiko Kawaguchi, Daisuke Ichikawa, Shinji Furuya, Katsutoshi Shoda, Makoto Sudo, Naoki Ashizawa, Naohiro Hosomura, Hidetake Amemiya, Hidenori Akaike, Kazuyoshi Hirayama, Ryo Saito, Atsushi Yamamoto, Shingo Inoue, Hiroshi Kono, and Koichi Takiguchi
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Male ,Sarcopenia ,medicine.medical_specialty ,Colorectal cancer ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Stage ii ,Cancer recurrence ,Gastroenterology ,Computed tomographic ,Postoperative Complications ,Internal medicine ,Overall survival ,Humans ,Medicine ,Psoas Muscles ,Retrospective Studies ,Chemotherapy ,Nutrition and Dietetics ,business.industry ,Nutritional status ,Prognosis ,musculoskeletal system ,medicine.disease ,body regions ,Female ,Colorectal Neoplasms ,business ,human activities - Abstract
Objectives Nutritional status significantly influences postoperative prognosis in gastrointestinal cancers. It has been evaluated using sarcopenia before treatments such as surgery and chemotherapy, despite constant changes in nutritional status. We consider that nutritional status at cancer recurrence is one of the important factors that affect treatment choice and intensity. This study evaluated the prognostic effects of improved postoperative nutritional status for people with colorectal cancer recurrence. Methods We enrolled 209 participants with pathologically confirmed stage II or III colorectal cancer who underwent radical resection. Sarcopenia was diagnosed using the psoas muscle index obtained from analysis of three-dimensional computed tomographic images. We adopted the cutoff value that was proposed by Hamaguchi et al. (psoas muscle index Results Among the 209 participants, 81 (38.8%) had preoperative sarcopenia; this group had significantly lower overall survival than those without sarcopenia (P = 0.028). Colorectal cancer recurred in 48 participants. Of those 46, sarcopenia was evaluated at the time of recurrence; 19 of those 46 had preoperative sarcopenia. Preoperative sarcopenia did not affect the cancer recurrence ratio (sarcopenia, 23.5%; non-sarcopenia, 21.3%; P = 0.893). The sarcopenia-improvement group had higher overall survival than the sarcopenia-continuation group (P = 0.042). Conclusions Among participants with preoperative sarcopenia, the prognosis at the time of recurrence improved for the sarcopenia-improvement group compared to the sarcopenia-continuation group. In people with colorectal cancer and sarcopenia, nutritional management is important not only before but also after surgery.
- Published
- 2021
45. P51-1 A case of recurrent breast cancer with liver metastasis successfully treated with multi-disciplinary
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Masato Ohmori, Hiroshi Kono, Ayako Kimura, Hidenori Akaike, Daisuke Ichikawa, Yuko Nakayama, Shinji Furuya, Yoshihiko Kawaguchi, Naohiro Hosomura, Makoto Sudo, Katsutoshi Shoda, Shingo Inoue, Hiromiti Kawaida, and Hidetake Amemiya
- Subjects
Oncology ,medicine.medical_specialty ,Multi disciplinary ,business.industry ,Internal medicine ,medicine ,Hematology ,medicine.disease ,business ,Recurrent breast cancer ,Metastasis - Published
- 2021
46. Valosin-Containing Protein/p97 as a Novel Therapeutic Target in Acute Lymphoblastic Leukemia
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Qi Cao, De-Chen Lin, H. Phillip Koeffler, Sumiko Takao, Mark Rolfe, Ronan Le Moigne, Markus Müschen, Sigal Gery, Gabriele Gugliotta, Haibo Sun, Makoto Sudo, Michele Cavo, Gugliotta, Gabriele, Sudo, Makoto, Cao, Qi, Lin, De-chen, Sun, Haibo, Takao, Sumiko, Le Moigne, Ronan, Rolfe, Mark, Gery, Sigal, Müschen, Marku, Cavo, Michele, and Koeffler, H. Phillip
- Subjects
PEI, polyethylenimine ,0301 basic medicine ,Cancer Research ,Apoptosis ,ERAD, ER-associated degradation ,MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ,Gene Knockout Techniques ,chemistry.chemical_compound ,UPR, unfolded protein response ,Valosin Containing Protein ,Molecular Targeted Therapy ,Endoplasmic Reticulum Chaperone BiP ,Tumor ,Cell Cycle ,Drug Synergism ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Endoplasmic Reticulum Stress ,Biochemistry ,Signal Transduction ,medicine.drug ,Original article ,Vincristine ,Programmed cell death ,XBP1 ,HNA, 2-hydroxy-1-naphthaldehyde ,Cell Survival ,Clinical Sciences ,B-ALL, B acute lymphoblastic leukemia ,Antineoplastic Agents ,Biology ,lcsh:RC254-282 ,4-OHT, 4-hydroxy tamoxifen ,Cell Line ,ER, endoplasmic reticulum ,PI, propidium iodide ,03 medical and health sciences ,Cell Line, Tumor ,medicine ,Humans ,Oncology & Carcinogenesis ,Propidium iodide ,Cell Proliferation ,Endoplasmic reticulum ,030104 developmental biology ,Gene Expression Regulation ,chemistry ,Cell culture ,Unfolded Protein Response ,Cancer research ,Unfolded protein response ,Biomarkers - Abstract
B acute lymphoblastic leukemia (B-ALL) cells are distinctively vulnerable to endoplasmic reticulum (ER) stress. Recently, inhibition of p97 was shown to induce ER stress and subsequently cell death in solid tumors and in multiple myeloma. We investigated the role of a novel, orally available, p97 inhibitor (CB-5083; Cleave Biosciences) in B-ALL. CB-5083 induced a significant reduction in viability in 10 human B-ALL cell lines, harboring the most common fusion-genes involved in pediatric and adult B-ALL, with IC50s ranging from 0.34 to 0.76 μM. Moreover, CB-5083 significantly reduced the colony formation of OP1 and NALM6 cells. Early and strong induction of apoptosis was demonstrated in BALL1 and OP1 cells, together with a robust cleavage of PARP. CB-5083 induced ER stress, as documented through: 1) prominent expression of chaperones (GRP78, GRP94, PDI, DNAJC3, and DNAJB9); 2) increased activation of IRE1-alpha, as demonstrated by the splicing of XBP1; and 3) activation of PERK, which resulted in a significant overexpression of CHOP, and its downstream genes. CB-5083 reduced the viability also in GRP78−/−, GRP94−/−, and XBP1−/− cells, suggesting that none of these proteins alone was strictly required for CB-5083 activity. Moreover, we showed that the absence of XBP1 (XBP1−/−) increased the sensitivity to CB-5083, leading to the hypothesis that XBP1 splicing counteracts the activity of CB-5083, probably mitigating ER stress. Finally, vincristine was synergistic with CB-5083 in both BALL1 and OP1 cells. In summary, the targeting of p97 with CB-5083 is a novel promising therapeutic approach that should be further evaluated in B-ALL.
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- 2017
47. Targeting the vulnerability to NAD+ depletion in B-cell acute lymphoblastic leukemia
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William Senapedis, Markus Müschen, Anand Mayakonda, D-C Lin, Makoto Sudo, H P Koeffler, Michael Lill, E Park, Sigal Gery, Sumiko Takao, Erkan Baloglu, L-W Ding, Y. Y. Jiang, Wenwen Chien, Q-Y Sun, Liang Xu, Ye Chen, and Vikas Madan
- Subjects
0301 basic medicine ,chemistry.chemical_classification ,Cancer Research ,Cell growth ,Kinase ,Nicotinamide phosphoribosyltransferase ,Hematology ,Nicotinamide adenine dinucleotide ,Biology ,Serine ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Enzyme ,Oncology ,chemistry ,Biochemistry ,Cell culture ,Cancer research ,NAD+ kinase - Abstract
Although substantial progress has been made in the treatment of B-cell acute lymphoblastic leukemia (B-ALL), the prognosis of patients with either refractory or relapsed B-ALL remains dismal. Novel therapeutic strategies are needed to improve the outcome of these patients. KPT-9274 is a novel dual inhibitor of p21-activated kinase 4 (PAK4) and nicotinamide phosphoribosyltransferase (NAMPT). PAK4 is a serine/threonine kinase that regulates a variety of fundamental cellular processes. NAMPT is a rate-limiting enzyme in the salvage biosynthesis pathway of nicotinamide adenine dinucleotide (NAD) that plays a vital role in energy metabolism. Here, we show that KPT-9274 strongly inhibits B-ALL cell growth regardless of cytogenetic abnormalities. We also demonstrate the potent in vivo efficacy and tolerability of KPT-9274 in a patient-derived xenograft murine model of B-ALL. Interestingly, although KPT-9274 is a dual PAK4/NAMPT inhibitor, B-ALL cell growth inhibition by KPT-9274 was largely abolished with nicotinic acid supplementation, indicating that the inhibitory effects on B-ALL cells are mainly exerted by NAD+ depletion through NAMPT inhibition. Moreover, we have found that the extreme susceptibility of B-ALL cells to NAMPT inhibition is related to the reduced cellular NAD+ reserve. NAD+ depletion may be a promising alternative approach to treating patients with B-ALL.
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- 2017
48. [Effects of Preoperative Skeletal Muscle Mass on the Prognosis of StageⅡ and Ⅲ Colorectal Cancer]
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Yuuki, Nakata, Shinji, Furuya, Kouichi, Takiguchi, Naoki, Ashizawa, Hiroki, Shimizu, Hidenori, Akaike, Naohiro, Hosomura, Yoshihiko, Kawaguchi, Hidetake, Amemiya, Makoto, Sudo, Hiromichi, Kawaida, Shingo, Inoue, Hiroshi, Kono, and Daisuke, Ichikawa
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Male ,Nutrition Assessment ,Humans ,Female ,Colorectal Neoplasms ,Muscle, Skeletal ,Prognosis ,Retrospective Studies - Abstract
Some studies reported that the decrease in skeletal muscle mass worsens the immune and nutritional status and related to the poor prognosis in colorectal cancer. We examined the relationship of skeletal muscle mass, immune and nutritional index, and outcome in patients with colorectal cancer at our hospital.We retrospectively analyzed 196 patients of cStageⅡ andⅢ colorectal cancer who underwent curative surgery in our institution between 2007 and 2013. The cross-sectional area of the psoas muscle at the level of the third lumbar vertebra on preoperative computed tomography was assessed to calculate the psoas muscle index(PMI). Patients are divided into high PMIgroup(H-group)and low PMIgroup (L-group)with cut off value(6.36 cm / / 2/m2 for males and 3.92 cm2/m2 for females). Patient background, tumor factor, overall survival(OS), recurrence free survival(RFS)were examined retrospectively.There were 119 cases in H-group and 77 cases in L-group. Significant differences were recognized in gender, age, Alb value, BMI, and adjuvant chemotherapy between 2 groups. The 5-year survival rate was significantly different from 82.8% in H-group and 70.3% in L-group(p0.01). The 5-year recurrence-free survival rate was 74.0%in the H-group, and 68.3%in the L-group (p=0.46). Univariate and multivariate analysis showed that age(OR: 1.90, p0.01), high CEA(OR: 0.012, p0.05), depth of invasion(OR: 2.19, p0.05), lymph node metastasis(OR: 2.21, p0.01), and preoperative low PMI(OR: 2.05, p0.01), were significantly related to decrease of OS.Preoperative PMIsuggested to become prognostic factors in Stage Ⅱ and Ⅲ colorectal cancer.
- Published
- 2019
49. [Investigation of Lateral Lymph Node Dissection for Rectal Cancer in Our Department]
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Yoshihiro, Akazawa, Makoto, Sudo, Shinji, Furuya, Yuuki, Nakata, Hiroki, Shimizu, Hidenori, Akaike, Naohiro, Hosomura, Yoshihiko, Kawaguchi, Hidetake, Amemiya, Hiromichi, Kawaida, Shingo, Inoue, Hiroshi, Kono, Hiroshi, Iino, Hideki, Fujii, and Daisuke, Ichikawa
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Japan ,Rectal Neoplasms ,Lymphatic Metastasis ,Humans ,Lymph Node Excision ,Lymph Nodes - Abstract
No clear policy has been established in Japan for proper lymph node dissection for rectal cancer. In our department, we examined the frequency of lateral lymph node metastasis, its treatment outcomes, and whether lateral dissection can narrow down necessary cases. In 10 years from 2003 to 2013, 98 cases of lower rectal cancer surgically treated in our department were examined. The clinicopathological factors in these cases were examined, and the risk factors were examined based on their correlation with the presence or absence of lateral lymph node metastasis. Based on the postoperative prognostic analysis, the dissection effect index(metastasis positive rate×5-year survival rate in cases with positive metastases)was also examined. Forty-three lateral lymph node dissections were performed. Cases involving a circumferential resection margin (CRM)of 1mm or less had significantly more lateral lymph node metastases. In the prognostic analysis, the 5-year survival rate of lateral lymph node metastasis-positive cases was 19%, and the dissection effect index was 3.5. It was suggested that CRM-positive patients had a higher risk of lateral lymph node metastasis before surgery. However, considering the results of this study and the results of JCOG0212, the presence or absence of lateral lymph node metastasis may be a prognostic predictor, although the prognostic improvement effect by dissection is considered to be limited.
- Published
- 2019
50. Differential miRNA Expression in Basaloid Squamous Cell Carcinoma of the Oesophagus: miR-3687 Targets
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Jiro, Nakamura, Shinji, Furuya, Kotaro, Hagio, Suguru, Maruyama, Kensuke, Shiraishi, Hiroki, Shimizu, Hidenori, Akaike, Naohiro, Hosomura, Yoshihiko, Kawaguchi, Hidetake, Amemiya, Hiromichi, Kawaida, Makoto, Sudo, Hiroshi, Kono, and Daisuke, Ichikawa
- Subjects
Male ,Esophageal Neoplasms ,Gene Expression Profiling ,Membrane Proteins ,Middle Aged ,Up-Regulation ,Esophagectomy ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Cell Line, Tumor ,Carcinoma, Squamous Cell ,Disease Progression ,Humans ,Female ,Receptors, Progesterone ,Aged ,Cell Proliferation ,Neoplasm Staging ,Oligonucleotide Array Sequence Analysis - Abstract
Basaloid squamous cell carcinoma of the oesophagus (BSCCE) has poorer prognosis than conventional oesophageal squamous cell carcinoma (ESCC). This study is the first report on highly expressed miRNAs in BSCCE and their target genes.BSCCE and ESCC patients who underwent esophagectomy were selected for this study. Total RNA was extracted from formalin-fixed paraffin-embedded blocks to examine expression of miRNAs and target genes. miRNA mimic or inhibitor transfected cells were used in validation experiments. miRNA and mRNA quantification were performed by quantitative reverse transcription polymerase chain reaction (qRT-PCR).miRNA microarray analysis revealed four candidate miRNAs. Further investigations including cell line experiments demonstrated that miR-3687 was a candidate miRNA and progesterone receptor membrane component2 (PGRMC2) was its target gene. PGRMC2 was found to be related to cell proliferation and local progression.miR-3687 may be a candidate miRNA conferring BSCCE aggressiveness, and PGRMC2 is one of its target genes.
- Published
- 2019
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