Back to Search
Start Over
Differential miRNA Expression in Basaloid Squamous Cell Carcinoma of the Oesophagus: miR-3687 Targets PGRMC2
- Source :
- Anticancer Research. 39:6471-6478
- Publication Year :
- 2019
- Publisher :
- Anticancer Research USA Inc., 2019.
-
Abstract
- Background/aim Basaloid squamous cell carcinoma of the oesophagus (BSCCE) has poorer prognosis than conventional oesophageal squamous cell carcinoma (ESCC). This study is the first report on highly expressed miRNAs in BSCCE and their target genes. Materials and methods BSCCE and ESCC patients who underwent esophagectomy were selected for this study. Total RNA was extracted from formalin-fixed paraffin-embedded blocks to examine expression of miRNAs and target genes. miRNA mimic or inhibitor transfected cells were used in validation experiments. miRNA and mRNA quantification were performed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results miRNA microarray analysis revealed four candidate miRNAs. Further investigations including cell line experiments demonstrated that miR-3687 was a candidate miRNA and progesterone receptor membrane component2 (PGRMC2) was its target gene. PGRMC2 was found to be related to cell proliferation and local progression. Conclusion miR-3687 may be a candidate miRNA conferring BSCCE aggressiveness, and PGRMC2 is one of its target genes.
- Subjects :
- Cancer Research
Messenger RNA
Cell growth
General Medicine
Transfection
Biology
Reverse transcription polymerase chain reaction
03 medical and health sciences
0302 clinical medicine
Oncology
030220 oncology & carcinogenesis
microRNA
Progesterone receptor
Cancer research
Basaloid Squamous Cell Carcinoma
Gene
Subjects
Details
- ISSN :
- 17917530 and 02507005
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Anticancer Research
- Accession number :
- edsair.doi...........f705115a86b20d5a2aa3426a315f90e6