49 results on '"Huitao Zhang"'
Search Results
2. Nitratireductor luteus sp. nov. isolated from saline-alkali land
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Xinping, Yang, Liuyan, Zhou, Xiaowu, Wang, Zhifang, Wang, Pengbin, Li, Jinping, Dai, Huitao, Zhang, and Yuqing, Xie
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General Medicine ,Molecular Biology ,Microbiology - Abstract
The Gram-staining negative, oxidase and catalase negative strain KC-ST17
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- 2022
3. Design and implementation of open laboratory management system based on 2DPCA face identification technology
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Huitao Zhang, Li Yang, Jingwu Sun, and Zhiguo Liu
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Computational Mathematics ,General Engineering ,Computer Science Applications - Abstract
According to the current management status of laboratories, an open laboratory management system based on 2DPCA face recognition technology is developed. Within this system, the environment of the lab is monitored from multiple aspects such as temperature and humidity, light intensity, PM2.5. The ZigBee module is adopted to realize the check-in and access control, which can automatically record the attendance of laboratory personnel and save the inconvenience of recording information manually. 2DPCA face recognition technology is used to ensure the security and theft prevention of the laboratory. The nodes composed of IoT, various sensors and microcontrollers help with the collection, transmission and processing of data between modules. Simultaneously, the IoT website is developed to record and display the environmental information of the lab, and the information will be published to the public network in real time through the ipv6 web server, thus allowing the users to view the usage of the work station through the net page on their cell phones. The innovation of this system design lies in the involvement of 2DPCA face recognition technology, which not only ensures the laboratory safety but also solves the problem of students not being able to enter due to the dim lighting and low resolution in the evening. Besides, the 2DPCA technology’ ability to meet the demand of open laboratory management system is verified by the simulation and experimental verification.
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- 2022
4. Hemodialysis Arteriovenous Fistula Dysfunction: Retrospective Comparison of Post-thrombotic Percutaneous Endovascular Interventions with Pre-emptive Angioplasty
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Xinyan Hu, Bing Li, Junjie Mao, Xiaojun Hu, Jingjing Zhang, Hui Guo, Dashuai Wang, Yongyu Zhang, Jianan He, Ni Zhao, Huitao Zhang, and Pengfei Pang
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Angioplasty ,Graft Occlusion, Vascular ,Thrombosis ,General Medicine ,Urokinase-Type Plasminogen Activator ,Arteriovenous Shunt, Surgical ,Treatment Outcome ,Renal Dialysis ,Arteriovenous Fistula ,Humans ,Surgery ,Cardiology and Cardiovascular Medicine ,Angioplasty, Balloon ,Vascular Patency ,Retrospective Studies - Abstract
We aimed to compare the clinical outcomes of pre-emptive angioplasty versus post-thrombotic percutaneous endovascular restoration of dysfunctional arteriovenous fistula (AVF).This retrospective study reviewed the data from 80 patients who underwent 114 endovascular interventions for a malfunctioning AVF from July 2016 to August 2019. Stenotic AVFs were treated with pre-emptive angioplasty. Thrombosed AVFs were treated with percutaneous pharmacomechanical fibrinolysis with urokinase used only during the operation or continuously infused. The differences in patency rates were evaluated using the Kaplan-Meier method. In addition, univariate and multivariate regression Cox models were used to determine influential factors on the postintervention primary patency.Post-thrombotic interventions and pre-emptive angioplasty yielded statistically similar rates in clinical success (100 vs. 100%), anatomic success (94 vs. 89%; P = 0.52), complication (4 vs. 11%; P = 0.29), as well as postintervention primary, assisted primary and secondary patency (P = 0.80; 0.57; 0.57). The use of pre-emptive angioplasty was associated with reduced total cost (¥25,108 vs. ¥30,833, P 0.001). The patients who used urokinase only during the operation prolonged both the primary and assisted primary patency (P = 0.02; 0.002), while those with continuous infusion of urokinase had worst patency rates and high costs (¥39,275 vs. ¥25,108 vs. ¥27,140, P 0.001). Compared with the other locations, dysfunction in the anastomotic or juxta-anastomotic segment (HR = 0.41, P = 0.001) was associated with prolonged postintervention primary patency.No clinical outcome differences were found between the post-thrombotic percutaneous endovascular interventions and pre-emptive angioplasty. However, pre-emptive angioplasty decreased access expenditure.
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- 2022
5. Reconstruction Algorithm-Based CT Imaging for the Diagnosis of Hepatic Ascites
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Huitao Zhang, Wenhao Lv, Haofeng Diao, and Li Shang
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Article Subject ,General Immunology and Microbiology ,Applied Mathematics ,Ascites ,Water ,General Medicine ,General Biochemistry, Genetics and Molecular Biology ,Artificial Intelligence ,Modeling and Simulation ,Humans ,Radiographic Image Interpretation, Computer-Assisted ,Calcium ,Tomography, X-Ray Computed ,Algorithms ,Iodine - Abstract
The study was aimed at exploring the diagnostic value of artificial intelligence reconstruction algorithm combined with CT image parameters on hepatic ascites, expected to provide a reference for the etiological evaluation of clinical abdominal effusion. Specifically, the adaptive iterative hard threshold (AIHT) algorithm for CT image reconstruction was proposed. Then, 100 patients with peritoneal effusion were selected as the research subjects. After 8 cases were excluded, the remaining was divided into 50 cases of the S1 group (hepatic ascites) and 42 cases of the D0 group (cancerous peritoneal effusion). Gemstone energy spectrum CT scanning was performed on all patients, and CT image parameters of the two groups were compared. It was found that CT value of mixed energy, CT value of 60-100 KeV single energy, concentration value of water (calcium), concentration value of water (iodine), and slope of energy spectrum curve in the S1 group were significantly lower than those in the D0 group ( P < 0.05 ). The effective atomic number in the S1 group was significantly higher than that in the D0 group ( P < 0.05 ). Of the 50 patients in the S1 group, 3 (6%) had an ascending and 47 (94%) had a descending spectral curve. Of the 42 patients in the D0 group, 37 (88.1%) had an ascending and 5 (11.9%) had a descending spectral curve. The sensitivity and specificity of water (iodine) were 0.927 and 0.836, respectively. The sensitivity and specificity of water (calcium) were 0.863 and 0.887, respectively. For different scan ranges ([0,90]; [0,120]), root mean square error (RMSE) of AIHT reconstructed image was significantly smaller than that of traditional algorithm, while peak signal-to-noise ratio (PSNR) was opposite. The differences were statistically significant ( P < 0.05 ). In conclusion, AIHT-based CT images can better display the distribution of hepatic ascites, and the parameters of CT value, effective atomic number, water (iodine), water (calcium), and spectral curve can all provide help for the identification of hepatic ascites. Especially, water (iodine) and water (calcium) demonstrated high diagnostic performance of hepatic ascites.
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- 2022
6. Analysis of Spatial Structure and Filling Mechanism of Paleokarst Conduit Against a Background of Denudation: A Case Study in the Tahe Oilfield, Tarim Basin
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Chenhe Zhang, Zhiqian Gao, Tailiang Fan, Duan Wei, Jun Wua, Debin Yang, Jinxian Liu, Huitao Zhang, Bo Li, Zhe Chang, Yu Gu, Ming Jiang, Ze Gao, Aolin Wang, and Qianxi Zou
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- 2023
7. Protein Post-translational Modification Site Prediction using Deep Learning
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Yujuan Deng, Yunfang Fu, Huitao Zhang, Xin Liu, and Zhiguo Liu
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General Earth and Planetary Sciences ,General Environmental Science - Published
- 2022
8. Encapsulating has-miR-29a into HuMSCs-derived Exosomes by a Nanoengineering Platform to Efficiently Alleviate Aβ1-42 Expression in APPswe-SH-SY5Y Cells
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Huitao Zhang, Hui Yang, Rui Hao, Zitong Yu, Shi Hu, Jingyi Ren, Juan Ding, and Yi Zhang
- Abstract
Background Exosomes derived from human umbilical cord mesenchymal stem cells (HuMSCs) have been utilized as a potential therapeutic candidate to alleviate amyloid β-protein (Aβ) expression in APP/PS1 or 5XFAD transgenic mice due to their low immunogenicity and extensive sources. Accumulated evidence also suggests that encysting appropriate cargos into exosomes can more effectively reduce Aβ expression by interfering with the metabolism of β-Amyloid precursor protein (APP). Therefore, exosomes loaded with bioactive substances to reduce Aβ expression have attracted much attention. A new strategy to load therapeutic exogenous cargoes of interest into exosomes in a high-throughput and straightforward fashion is desired. Results In this study, neuroblastoma SH-SY5Y cells were stably transfected with Swedish mutant APP695K, 595N plasmid to construct a cell model of Aβ overexpression. Then extracted Exosomes from HuMSCs and loaded with micro (miR)-29a via a microfluidic exosome nanoengineering platform (ExoNP) to target a post-transcriptional modification of the β-secretase1 (β-site APP cleaving enzyme 1, BACE1) gene to prevent protein expression. Finally, processed exosomes were utilized to treat APPswe-SH-SY5Y cells and decrease the expression of Aβ1−42 and BACE1 and further alleviated apoptosis. Conclusion HuMSCs-derived exosomes loaded with hsa-miR-29a via ExoNP to efficiently deliver to APPswe-SH-SY5Y cells and alleviate Aβ1−42 expression and promote cell survival.
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- 2022
9. Chattering suppression of the sliding mode observer for marine electric propulsion motor based on piecewise power function
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Xiangfeng Li, Shengqiang Zhan, Fang Guo, Zidan Zhuang, Huitao Zhang, Hui Liao, and Lili Qu
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Economics and Econometrics ,Fuel Technology ,Renewable Energy, Sustainability and the Environment ,Energy Engineering and Power Technology - Abstract
The sliding mode observer (SMO)–based sensorless control is essentially a discontinuous switching control algorithm. Therefore, there is large speed or torque chattering in the permanent magnet synchronous motor (PMSM) with existing sliding mode observer–based control methods. In order to solve this problem well, an algorithm based on an improved sliding mode observer is proposed. In detail, the piecewise power function approach is used to replace the traditional ones, that is, the sgn function or the sigmoid function approach. Simulation verifications are conducted to validate the effectiveness of the proposed observer. It shows that the piecewise power function–based sliding mode observer algorithm has obvious advantages in the stability of the three-phase current, namely, it improves the estimation accuracy of the rotor position, rotor speed, and dynamic response to various loads. For further verification, the proposed sliding mode observer algorithm is implemented and verified in a marine electric propulsion motor with a DSP chip of TMS320F28377D. Experimental results validate that the piecewise power function–based sliding mode observer algorithm has high control accuracy and fewer fluctuations, which are all vital in marine engineering.
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- 2022
10. One-Step Method for Material Quantitation Using In-Line Tomography With Single Scanning
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Suyu Liao, Shiwo Deng, Yining Zhu, Huitao Zhang, Peiping Zhu, Kai Zhang, and Xing Zhao
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Image and Video Processing (eess.IV) ,FOS: Electrical engineering, electronic engineering, information engineering ,Biomedical Engineering ,FOS: Physical sciences ,Medical Physics (physics.med-ph) ,Electrical Engineering and Systems Science - Image and Video Processing ,Physics - Medical Physics - Abstract
Objective: Quantitative technique based on In-line phase-contrast computed tomography with single scanning attracts more attention in application due to the flexibility of the implementation. However, the quantitative results usually suffer from artifacts and noise, since the phase retrieval and reconstruction are independent ("two-steps") without feedback from the original data. Our goal is to develop a method for material quantitative imaging based on a priori information specifically for the single-scanning data. Method: An iterative method that directly reconstructs the refractive index decrement delta and imaginary beta of the object from observed data ("one-step") within single object-to-detector distance (ODD) scanning. Simultaneously, high-quality quantitative reconstruction results are obtained by using a linear approximation that achieves material decomposition in the iterative process. Results: By comparing the equivalent atomic number of the material decomposition results in experiments, the accuracy of the proposed method is greater than 97.2%. Conclusion: The quantitative reconstruction and decomposition results are effectively improved, and there are feedback and corrections during the iteration, which effectively reduce the impact of noise and errors. Significance: This algorithm has the potential for quantitative imaging research, especially for imaging live samples and human breast preclinical studies.
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- 2022
11. The effect of bacteria inoculation on the lignocellulose degradation and the microbial properties during cow dung composting
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Liuyan Zhou, Xinping Yang, Yuqing Xie, Xiaowu Wang, Jinping Dai, Zhifang Wang, Huitao Zhang, and Lei Feng
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The goal of this work was to explore the potential effects of bacteria inoculation on the lignocellulose degradation and the underlying microbial mechanism during cow dung composting. The results suggested that bacteria inoculation better-accelerated temperature rose and increased thermophilic phase. Compared to the control (without inoculation, CK), the cellulose, hemicellulose, and lignin degradation rates of treated group (EG) increased from 53.3% to 70.0%, 50.2% to 61.3% and 46.4% to 60.0%, respectively. The microbial community structure and diversity were obviously changed by inoculation. Moreover, inoculation contributed to modulate the key compost microbial functional populations linking to the degradation of lignocelluloses by stamp analysis. Correlation matrix analysis indicated that the expression of bacterial lingo-cellulolytic enzymes were closely related to the key microbial functional populations. Overall, the results confirmed the importance of bacteria inoculation and have important implication for promoting the efficiency and quality of cow dung compost.
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- 2022
12. MALDI-MS-based biomarker analysis of extracellular vesicles from human lung carcinoma cells
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Chao Zhao, Wei Li, Zitong Yu, Qian Luo, Renjie Zhang, Huitao Zhang, Hui Yang, and Shi Hu
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0303 health sciences ,biology ,Cell growth ,Chemistry ,General Chemical Engineering ,010401 analytical chemistry ,S100A10 ,General Chemistry ,medicine.disease ,01 natural sciences ,RPS27A ,0104 chemical sciences ,Ribosome assembly ,03 medical and health sciences ,Cell culture ,Cancer cell ,biology.protein ,medicine ,Cancer research ,Lung cancer ,Fetal bovine serum ,030304 developmental biology - Abstract
Extracellular vesicles (EVs) are actively secreted by mammalian cells. They are increasingly recognized as promising circulating biomarkers of disease progression. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is currently one of the most powerful techniques for the rapid analysis of biological samples, especially for discovering biomarkers for disease diagnosis and prognosis. It is unclear what cell culture medium components and EV isolation methods are suitable for MALDI-TOF MS analysis. Using a human lung carcinoma cell line (A549), we investigated and optimized the critical experimental conditions for EVs' protein profiling by combining differential ultracentrifugation and MALDI-TOF MS. The results demonstrated that medium components and ultracentrifugation procedures to extract EVs played important roles in MS detection. Compared with EV-depleted serum and normal serum medium, conditioned medium with 2% fetal bovine serum in this study maintained cell proliferation and displayed significant protein profiling of EVs. RPS27A (ribosomal protein), which plays an essential role in mRNA translation and ribosome assembly for the differentiation of cancer cells, was detected from the EVs of lung cancer cells associated with cancer cell migration and invasion. We also found the known tumor diagnosis marker, which is S100A10_S100 calcium-binding protein A10. Therefore, MALDI-TOF MS-based EV analysis with optimized experimental protocols can contribute to future development of rapid screening techniques of protein biomarkers associated with early cancer diagnosis.
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- 2021
13. Multi-scale dilated dense reconstruction network for limited-angle computed tomography
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Haichuan Zhou, Yining Zhu, Huitao Zhang, Xing Zhao, and Peng Zhang
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Radiological and Ultrasound Technology ,Radiology, Nuclear Medicine and imaging - Abstract
Objective. Due to the incomplete projection data, the image reconstructed by limited-angle computed tomography (CT) usually suffers from significant artifacts, by which the structural details are heavily blurred. In this study, we aim to develop a novel approach to improve the limited-angle CT reconstruction performance, especially for the narrow scanning angular range. Approach. A deep learning based iterative framework for limited-angle tomography is proposed, which is named multi-scale dilated dense reconstruction network (MSDDRNet). The MSDDRNet utilizes a multi-scale dilated dense convolution neural network (MSDD-CNN) with conventional reconstruction algorithm for predicting image from incomplete projection data. The MSDD-CNN enhances the image features in the network by merging the DenseNet-Like structure, which serves to restore invisible singularities and reduce artifacts, as well as introducing constraints on the projection domain data into the iterative process to achieve better image detail recovery. Additionally, to improve the training speed of the network, we use a strategy of pre-training and model migration. Main results. Numerical experiments demonstrate that the proposed MSDDRNet performs well in terms of artifact correction, noise reduction and structure recovery compared to existing methods with limited scan angles, and we also extend the proposed method to more general scanning condition and other application such as dental CT data. Significance. The proposed method is a general framework, which can be applied to other CT problems, such as low dose CT, sparse-data CT and spectral CT.
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- 2023
14. Mechanical stimulation on a microfluidic device to highly enhance small extracellular vesicle secretion of mesenchymal stem cells
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Rui Hao, Shi Hu, Huitao Zhang, Xi Chen, Zitong Yu, Jingyi Ren, Hang Guo, and Hui Yang
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Biomaterials ,Biomedical Engineering ,Bioengineering ,Cell Biology ,Molecular Biology ,Biotechnology - Abstract
Small extracellular vesicles (sEVs) are recognized as promising detection biomarkers and attractive delivery vehicles, showing great potential in diagnosis and treatment of diseases. However, the applications of sEVs are usually restricted by their poor secretion amount from donor cells under routine cell culture conditions, which is especially true for mesenchymal stem cells (MSCs) due to their limited expansion and early senescence. Here, a microfluidic device is proposed for boosting sEV secretion from MSCs derived from human fetal bone marrow (BM-MSCs). As the cells rapidly pass through a microfluidic channel with a series of narrow squeezing ridges, mechanical stimulation permeabilizes the cell membrane, thus promoting them to secrete more sEVs into extracellular space. In this study, the microfluidic device demonstrates that mechanical-squeezing effect could increase the secretion amount of sEVs from the BM-MSCs by approximately 4-fold, while maintaining cellular growth state of the stem cells. Further, the secreted sEVs are efficiently taken up by immortalized human corneal epithelial cells and accelerate corneal epithelial wound healing
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- 2023
15. Design of Anti-Lock Braking System for FSAE Racing Vehicle Based on New Slip Ratio Observation Method
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Yicai Liu, Da Wang, Luxu Liang, Huitao Zhang, Wenjie Wang, Mo Lv, Yiyang Wang, Yi Sun, and Shuai Yang
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- 2022
16. Effect of SARS-CoV-2 Infection on the Microbial Composition of Upper Airway
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Changli Tu, Xiaobin Zheng, Huitao Zhang, Jing Liu, Pengfei Pang, Yingjian Liang, Zhenguo Wang, Yiming Wang, Xiaojun Hu, Hong Shan, and Zhonghe Li
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0301 basic medicine ,Pharmacology ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Opportunistic infection ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,030106 microbiology ,Microbial composition ,macromolecular substances ,Biology ,medicine.disease ,medicine.disease_cause ,biology.organism_classification ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Infectious Diseases ,medicine ,Pharmacology (medical) ,030212 general & internal medicine ,Airway ,Bacteria ,Coronavirus - Abstract
Methods Forty-four COVID-19 patients (severe/critical: N = 8, non-severe: N = 36) were examined by next generation sequencing (NGS) of nasopharyngeal test paper to observe the effect of novel coronavirus infection to the microbial composition in upper airway. Results In these nasopharyngeal test paper samples, 38 kinds of bacteria, 10 kinds of viruses except SARS-CoV-2, nine kinds of fungi and three kinds of atypical pathogens had been found. There was some difference in microbial composition in the upper airway between severe and non-severe cases. Summary These results are important for us to study the effect of SARS-CoV-2 on the local microbial composition of upper airway and prevent opportunistic infection in severe patients.
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- 2020
17. Synthesis of MnO2 modified porous carbon spheres by preoxidation-assisted impregnation for catalytic oxidation of indoor formaldehyde
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Changming Zhang, Caimei Fan, Huitao Zhang, Rui Li, Yaqi Wang, Xiaochao Zhang, and Song Wen
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Materials science ,Scanning electron microscope ,Mechanical Engineering ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Catalysis ,Adsorption ,X-ray photoelectron spectroscopy ,Chemical engineering ,Catalytic oxidation ,Mechanics of Materials ,Desorption ,General Materials Science ,Fourier transform infrared spectroscopy ,Temperature-programmed reduction ,0210 nano-technology - Abstract
Resin-based porous carbon spheres with well dispersed MnO2 particles were sucessfully synthesised by steam activation and preoxidation-assisted impregnation of manganese nitrate salt. X-ray diffractometer (XRD), Fourier Transform Infrared spectroscopy (FT-IR), N2 adsorption–desorption, scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), H2 temperature programmed reduction (H2-TPR), O2 temperature-programmed desorption (O2-TPD) and in-situ diffuse reflectance fourier transform spectrometry (DRIFTS) were applied to characterize the obtained porous carbon spheres, and the HCHO adsorption and stability performances were evaluated in a fixed bed reactor. The results demonstrated that surface chemical properties and HCHO removal performances were significantly enhanced after the modification of preoxidation-assisted impregnation. The optimal manganese nitrate loading value, and relative humidity were 6 and 50%, respectively. Moreover, the higher HCHO concentration showed a smaller breakthrough time. The HCHO removal efficiency of ACS–O-6% Mn remained 100% even after reaction for 20 h at room temperature, while the average HCHO removal efficiency only declines by 0.2% compared with the first adsorption after 10 regeneration cycles. The in-situ DRIFTS results showed the smaller accumulation and faster desorption of intermediate products over the ACS–O-6% Mn. The HCHO removal mechanism analysis indicated that the enriched Mn3+ and surface active chemisorbed oxygen accounted for the excellent catalytic oxidation activity and stability.
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- 2020
18. Generation and phenotype analysis of CysLTR1 L118F mutant mice
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Huitao Zhang, Bing Li, Junjie Mao, Xiaojun Hu, Hong Shan, Bin Zhou, and Pengfei Pang
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Male ,0301 basic medicine ,Immunoprecipitation ,Mutant ,Biochemistry ,Calcium in biology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Animals ,Protein Interaction Domains and Motifs ,Molecular Biology ,Microinjection ,Receptors, Leukotriene ,Messenger RNA ,Behavior, Animal ,Chemistry ,Myocardium ,Point mutation ,Cell Biology ,Phenotype ,Cell biology ,Mice, Inbred C57BL ,Cysteinyl leukotriene receptor 1 ,030104 developmental biology ,030220 oncology & carcinogenesis ,Mutation ,Calcium ,Female ,Mutant Proteins - Abstract
Cysteinyl leukotrienes (CysLTs) are a group of eicosanoids that regulate the pathogenesis of various human diseases, mainly by signaling through the cysteinyl leukotriene receptor 1 (CysLTR1). The aim of this study was to generate and examine the phenotype of CysLTR1 L118F mutant mice. CysLTR1 L118F mutant mice were generated by the simultaneous microinjection of single guide RNA, Cas9 messenger RNA, and donor plasmid into fertilized mouse eggs. The morphological and behavioral characteristics of the resultant CysLTR1 L118F mutant mice were analyzed using an animal phenotype analysis platform, which included the assessment of body length, tail length, grip strength, and locomotor activity. Immunoprecipitation coupled with mass spectrometry was performed to identify CysLTR1-interacting proteins, and the intracellular calcium levels were determined using fluorometric imaging plate reader assays. The body length and tail length of CysLTR1 L118F mutant mice were significantly increased compared with wild-type mice. In addition, the grip strength and locomotor activity were remarkably elevated in L118F mutant mice compared with wild-type mice. Only three proteins were found to interact with both wild-type and CysLTR1 L118F proteins, whereas 4 and 13 additional proteins interacted exclusively with wild-type and mutant CysLTR1, respectively. Lastly, the responsiveness of cardiac muscle cells to CysLTs were significantly impaired by the L118F substitution in CysLTR1 proteins. The CysLTR1 L118F point mutation induced significant changes in the mouse morphology and behavior, which might be mediated by alterations of its protein interaction profile.
- Published
- 2019
19. Olmesartan Medoxomil, An Angiotensin II-Receptor Blocker, Ameliorates Renal Injury In db/db Mice
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Ye Zhu, Ze-Liang Li, Weiping Zhu, Tongxia Cui, Huitao Zhang, Ao Ding, Hua Zhang, and Hui Yang
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0301 basic medicine ,Pharmacology ,medicine.medical_specialty ,Kidney ,Angiotensin receptor ,business.industry ,Pharmaceutical Science ,Renal function ,medicine.disease ,Streptozotocin ,Angiotensin II ,Diabetic nephropathy ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Endocrinology ,030220 oncology & carcinogenesis ,Internal medicine ,Diabetes mellitus ,Drug Discovery ,Medicine ,business ,Olmesartan ,medicine.drug - Abstract
Background Diabetic nephropathy (DN) is a common complication of diabetes mellitus (DM) and also a major cause of end-stage renal disease (ESRD). Olmesartan medoxomil (OM) is an angiotensin II receptor blocker (ARB) and has been shown to exhibit renoprotective effects on a streptozotocin (STZ)-induced diabetic rat model. Yet, whether OM affects DN progression and renal injury in db/db mice, a type 2 diabetic murine model, has not been established. Methods Wild-type (n = 15) and db/db mice (n = 15) were treated with control saline or OM via oral gavage. The physiological and biochemical parameters were evaluated and histological examinations of kidney specimens were performed. Results Compared with saline-treated db/db mice, db/db mice administered with OM showed ameliorated diabetic physiological and biochemical parameters. In addition, OM decreased urinary albumin excretion and plasma creatinine level in db/db mice. Moreover, histologically, OM reduced glomerular hypertrophy and injury, and also ameliorated tubular injury, thus suggesting that OM improves renal function and minimizes renal pathological deterioration in db/db mice. Conclusion Our study reveals a beneficial role of OM in ameliorating DN in db/db mice, which is associated with its renoprotective function.
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- 2019
20. Generation of three lines from multiorgan venous and lymphatic defect syndrome patients
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Xiaojun Hu, Junjie Mao, Ke Zhang, Huitao Zhang, Dan Li, Bin Zhou, Hong Shan, Bing Li, and Pengfei Pang
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DEAD-box RNA Helicases ,QH301-705.5 ,Induced Pluripotent Stem Cells ,Mutation ,Humans ,Cell Differentiation ,Cell Biology ,General Medicine ,Syndrome ,Biology (General) ,Fibroblasts ,Developmental Biology - Abstract
Multiorgan venous and lymphatic defect (MOVLD) syndrome is a unique visceral vascular malformations with complex etiologies. In this study, primary skin fibroblasts were obtained from three MOVLD patients and reprogrammed into iPSCs by Yamanaka’s classical strategy. The MOVLD- iPSCs carrying the DDX24 p.Glu271Lys mutation were confirmed by Sanger sequencing. The pluripotency of MOVLD-iPSCs was verified by the specific molecular markers and gene expression, trilineage differentiation potential. The establishment of the MOVLD-iPSCs will provide a useful model for understanding the mechanisms involved the MOVLD and promoting the development of medical treatment.
- Published
- 2021
21. Angular sensitivity of an x-ray differential phase contrast imaging system with real and virtual source images
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Huitao Zhang, Peiping Zhu, Yongshuai Ge, Dong Liang, Hairong Zheng, Jianwei Chen, and Jiecheng Yang
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Physics ,business.industry ,Phase contrast microscopy ,X-ray ,Phase-contrast imaging ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,Virtual source ,law.invention ,010309 optics ,Optics ,law ,Thin lens ,0103 physical sciences ,Astronomical interferometer ,Sensitivity (control systems) ,0210 nano-technology ,business ,Differential phase contrast - Abstract
In this work, a novel, to the best of our knowledge, approach based on an x-ray thin lens imaging theory is proposed to predict the angular sensitivity responses of dual-phase-grating differential phase contrast (DPC) interferometers. Experimental validations have been performed to demonstrate the high accuracy of theoretical predictions using two different setups: one with real source images and the other with virtual source images. This new sensitivity calculation method is helpful to optimize the DPC imaging performance of a dual-phase-grating system.
- Published
- 2021
22. New troodontid theropod specimen from Inner Mongolia, China clarifies phylogenetic relationships of later-diverging small-bodied troodontids and paravian body size evolution
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Huitao Zhang, Qigao Jiangzuo, Lin Tan, Shuo Wang, Qingwei Tan, and Qiyue Zhang
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China ,Phylogenetic tree ,Evolutionary biology ,Animals ,Body Size ,Bayes Theorem ,Body size ,Biology ,Inner mongolia ,Ecology, Evolution, Behavior and Systematics ,Phylogeny ,Dinosaurs - Abstract
A new troodontid (LH PV39) recovered from the Upper Cretaceous Wulansuhai Formation, Inner Mongolia, China, is described, highlighting the dorsoventrally compressed sacral centra. The completely fused neurocentral junctions indicate that LH PV39 had reached adulthood at the time of death, but its size is nevertheless 20% smaller than that of the sympatric Philovenator, demonstrating that it is the second small-bodied troodontid recovered from the Wulansuhai Formation. Phylogenetic analyses scoring LH PV39 using different strategies and performed with different algorithms unambiguously recovered it as a troodontid. While the parsimony-based analysis scoring LH PV39 as an independent OTU with all of its available characteristics included recovered it as a basal troodontid, the Bayesian analysis suggests a closer relationship of LH PV39 to Almas and an unnamed troodontid from Ukhaa Tolgod, Mongolia (MPC-D100/1126+D100/3500). Body size analysis confirmed a single trend of gigantism throughout the evolution of troodontids, and suggests that the Late Cretaceous troodontids evolved in two directions: (i) several size-independent characteristics evolved while retaining the small sizes that are typical of the Early Cretaceous relatives, resulting in the Late Cretaceous small-bodied troodontids; and (ii) size-dependent characteristics (e.g., the elongation of the rostrum) evolved accompanying the size increase, resulting in large-bodied derived troodontids. The mosaic features of the Late Cretaceous small-bodied troodontids place them intermediate between their Early Cretaceous basal relatives and the Late Cretaceous large-bodied taxa in a well-resolved phylogeny, which is crucial for understanding the size and morphological evolution of troodontids.
- Published
- 2021
23. Mediation effect of AhR expression between polycyclic aromatic hydrocarbons exposure and oxidative DNA damage among Chinese occupational workers
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Yanli Liu, Jisheng Nie, Ye Fu, Jinzhu Zhao, Yingying Niu, Hongjie Zhang, Huitao Zhang, Jin Yang, and Aimin Yang
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Adult ,Male ,China ,Mediation (statistics) ,medicine.medical_specialty ,Health, Toxicology and Mutagenesis ,Urinary system ,Naphthols ,010501 environmental sciences ,Toxicology ,01 natural sciences ,law.invention ,Oxidative dna damage ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Asian People ,Naphthalenesulfonates ,law ,Occupational Exposure ,Internal medicine ,medicine ,Humans ,Deoxyguanosine ,Polycyclic Aromatic Hydrocarbons ,Coke ,Chromatography, High Pressure Liquid ,Polymerase chain reaction ,Carcinogen ,0105 earth and related environmental sciences ,Aspartic Acid ,Fluorenes ,Pyrenes ,biology ,Chemistry ,General Medicine ,Aryl hydrocarbon receptor ,030210 environmental & occupational health ,Pollution ,Reverse transcriptase ,Oxidative Stress ,Endocrinology ,Receptors, Aryl Hydrocarbon ,8-Hydroxy-2'-Deoxyguanosine ,Carcinogens ,biology.protein ,Environmental Pollutants ,DNA Damage - Abstract
Polycyclic aromatic hydrocarbons (PAH) are well-known to be carcinogenic and the mechanisms that it contributes to oxidative DNA damage and aryl hydrocarbon receptor (AhR)-dependent induction are also well understood. However, little is known about the associations between PAH exposure, AhR expression, and oxidative DNA damage. We investigated their associations of AhR expression and oxidative DNA damage related to PAH exposure among 310 workers from a coke-oven plant in China. Urine biomarkers of PAH exposure (2-hydroxynaphthalene, 2-NAP; 2-hydroxyfluorene, 2-FLU; 9-hydroxyphenanthren, 9-PHE; and 1-hydroxypyrene, 1-OHP) and a marker of oxidative damage (8-hydroxy- 2′- deoxyguanosine, 8-OHdG) were measured by high performance liquid chromatography. AhR expression in venous blood was measured by reverse transcription -polymerase chain reaction. The results showed that increasing levels of urinary 1-OHP was positively associated with high 8-OHdG (OR (95% CI) was 4.01 (1.41–11.45) for 4th quartile, compared with 1st quartile, P for trend = 0.013). The similar associations were also found between urinary 1-OHP and high-AhR expressions (4th vs. 1st quartile = 3.50, 95% CI: 1.24–9.87, P for trend = 0.029). A significant association between AhR expression and high 8-OHdG was also found (4th vs. 1st quartile = 2.44, 95% CI: 1.05–5.70, P for trend = 0.027). In addition, mediation analysis showed the AhR expression could explain 35.9% of the association of oxidative DNA damage related to PAH exposure. Our findings implicated that the association between PAH exposure and oxidative DNA damage may be mediated by AhR expression among Chinese occupational workers.
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- 2018
24. Population Pharmacokinetic Analysis of Mizoribine in Chinese Renal Transplant Recipients
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Qing-Ling Fu, Huitao Zhang, B. Ren, Long-shan Liu, Su-xiong Deng, Jiajia Jiang, Ji-guang Fei, C. Wang, Likun Chen, Jiang Qiu, Guanghao Chen, and J. Li
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Adult ,Male ,medicine.medical_specialty ,Population ,030232 urology & nephrology ,Urology ,Renal function ,Absorption (skin) ,030204 cardiovascular system & hematology ,Models, Biological ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Pharmacokinetics ,Humans ,Medicine ,education ,Aged ,Distribution Volume ,Transplantation ,education.field_of_study ,Mizoribine ,business.industry ,Bayes Theorem ,Middle Aged ,Kidney Transplantation ,Transplant Recipients ,Confidence interval ,Female ,Surgery ,Ribonucleosides ,business ,Immunosuppressive Agents ,medicine.drug - Abstract
Background We calculated the population pharmacokinetics of mizoribine in adult Chinese patients and compared the parameters with those of Japanese patients to determine whether there are any ethnic differences in blood concentration transition between these 2 populations. Methods The blood concentrations of mizoribine in 21 Chinese patients who were administered mizoribine after renal transplantation were measured at 304 time points. The absorption lag time, absorption rate constant, apparent distribution volume, and oral clearance were thereafter calculated and compared with the respective Japanese references. Results The absorption lag time, absorption rate constant, and apparent distribution volume calculated in this study were, respectively, 0.353 hour, 0.856 hour−1, and 0.776 L/kg. The oral clearance was calculated as 2.18 times the creatinine clearance using creatinine clearance as a function. The absorption rate constant, apparent distribution volume, and oral clearance are determinants of the maximum blood concentration, trough, and area under the blood concentration time curve. The relative absorption rate constant, apparent distribution volume, and oral clearance were 0.9-, 0.9-, and 1.2-fold, respectively, in Chinese patients compared with those in Japanese patients. These values are within the confidence limit, suggesting that there is no significant PK difference between the 2 ethnic groups. Conclusions Results of this study showed no ethnic difference in blood mizoribine concentration transition between Chinese and Japanese patients. In addition, the population pharmacokinetic parameters obtained in this study are useful in determining the initial dosage or in the Bayesian analysis of mizoribine concentrations using scarce time points.
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- 2018
25. Effect of Early Immunosuppression Therapy on De Novo Anti–Human-Leukocyte-Antigen Antibody After Kidney Transplantation
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Huitao Zhang, Gan Huang, Yuxin Zheng, Shuang Wang, Wuguo Deng, Qing-Ling Fu, Chun-Zhi Wang, Ronghai Deng, J. Li, Long-shan Liu, and Huiting Huang
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Adult ,Graft Rejection ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,030232 urology & nephrology ,030230 surgery ,Gastroenterology ,Mycophenolic acid ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,HLA Antigens ,Internal medicine ,medicine ,Humans ,Transplantation, Homologous ,Dosing ,Prospective cohort study ,Kidney transplantation ,Autoantibodies ,Retrospective Studies ,Transplantation ,business.industry ,Graft Survival ,Hazard ratio ,Retrospective cohort study ,Immunosuppression ,Mycophenolic Acid ,medicine.disease ,Kidney Transplantation ,Calcineurin ,Female ,Surgery ,business ,Immunosuppressive Agents ,medicine.drug - Abstract
The aim of the study was to investigate the effect of immunosuppression therapy early after kidney transplantation, particularly exposure of mycophenolic acid (MPA) and calcineurin inhibitor (CNI), on posttransplantation de novo HLA antibody production. Methods A single-center retrospective cohort study was performed at the First Affiliated Hospital of Sun Yat-sen University, enrolling the kidney transplant or pancreas-kidney transplant recipients who had surgery between January 2010 and February 2016. Results A total of 214 recipients were included in the study with a median follow-up period of 1.06 years. A total of 30 recipients (14.0%) were positive in HLA antibody detection posttransplant with a median follow-up period of 1.46 years. Ten recipients (4.7%) lost their allograft function during follow-up, and 6 of them (60%) developed de novo HLA antibody after graft failure. Multivariate analysis showed that acute rejection significantly increased the risk of de novo HLA antibody (hazard ratio [HR], 2.732). Intensified MPA dosing therapy reduced the risk by 59.8% (HR, 0.402); low-dose CNI therapy increased the risk by 33.3% (HR, 1.333), and the effect of extremely low-dose CNI therapy was even larger (HR, 2.242). Conclusion The risk of de novo HLA antibody can be decreased by reducing the risk of acute rejection. A tendency was seen in low-dose CNI therapy to increase the risk of de novo HLA antibody, but intensified MPA dosing therapy may provide an umbrella protection effect by reducing the risk. Prospective study was required to confirm the effects.
- Published
- 2018
26. Silica/antibody-conjugated microcomb electrode sensor for cardiac biomarker analysis
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Thangavel Lakshmipriya, Huitao Zhang, Yuan Seng Wu, Haitao Li, Jing Li, Xingzhou Zhao, Shujiang Song, Subash C. B. Gopinath, Zihan Xue, and Jinpeng Xu
- Subjects
010302 applied physics ,Detection limit ,medicine.medical_specialty ,biology ,Chemistry ,02 engineering and technology ,General Chemistry ,Gold standard (test) ,Conjugated system ,021001 nanoscience & nanotechnology ,medicine.disease ,01 natural sciences ,Silica nanoparticles ,Internal medicine ,0103 physical sciences ,medicine ,Cardiology ,biology.protein ,Interdigitated electrode ,General Materials Science ,Biomarker Analysis ,Myocardial infarction ,Antibody ,0210 nano-technology - Abstract
Myocardial infarction (MI), which is generally known as a heart attack, occurs when there is a lack of blood supply to the heart. Electrocardiogram (ECG) is commonly used for the initial screening of MI but is not sufficiently sensitive. With ECG, cardiac biomarkers help to efficiently assess MI. The cardiac troponin I level has a high correlation with MI and is one of the gold standard biomarkers to identify MI. This study focuses on detecting cardiac troponin I by its parent antibody on a microcomb interdigitated electrode (IDE) sensor. To improve the detection, silica nanoparticles (SiNps) were complexed with antibody and immobilized on the IDE sensing surface. Amine-modified SiNps help to hold more antibodies on the sensing surface and interact with more cardiac troponin I molecules, leading to sensitive detection with a limit of detection of 1 fM (24 pg/mL). Moreover, the control proteins were failed to specifically interact, indicating the genuine detection of cardiac troponin I. This study helps to more easily and efficiently quantify the cardiac troponin I level to diagnose MI.
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- 2021
27. Myocardial infarction biomarker C-reactive protein detection on nanocomposite aptasensor
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Jing Li, Xingzhou Zhao, Haitao Li, Shujiang Song, Huitao Zhang, and Jinpeng Xu
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Aptamer ,Biomedical Engineering ,Myocardial Infarction ,Early detection ,Metal Nanoparticles ,Bioengineering ,Biosensing Techniques ,Applied Microbiology and Biotechnology ,Nanocomposites ,Limit of Detection ,Drug Discovery ,Troponin I ,medicine ,Humans ,Myocardial infarction ,Electrodes ,biology ,Chemistry ,Process Chemistry and Technology ,C-reactive protein ,General Medicine ,Electrochemical Techniques ,Aptamers, Nucleotide ,medicine.disease ,C-Reactive Protein ,Interdigitated electrode ,biology.protein ,Molecular Medicine ,Biomarker (medicine) ,Gold ,Biomarkers ,Biotechnology ,Biomedical engineering - Abstract
Myocardial infarction (MI) is considered as one of the major life-threatening health issues worldwide. Growing number of cases every year is demanding rapid, portable, and early detection by the sensing devices for the identification of MI. This research work introduces a modified interdigitated electrode (IDE) sensing surface constructed with single-walled carbon nanotube (SWCN) to detect the cardiac biomarker, C-reactive protein (CRP). CRP-specific aptamer was conjugated with gold nanoparticle and attached on SWCN-constructed IDE surface. This probe-modified sensing surface has reached the limit of CRP detection to 10 pM on a linear regression curve with the regression coefficient of R² = 0.9223 [y = 0.9198x - 0.4326]. Further, control molecules, such as random aptamer sequence and nontarget cardiac biomarker (Troponin I), did not show the current response, indicating the specific CRP detection. This sensing strategy helps to detect the lower level of CRP and diagnose the MI at its earlier stages.
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- 2020
28. Projection-to-image transform frame: a lightweight block reconstruction network for computed tomography
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Genwei Ma, Xing Zhao, Yining Zhu, and Huitao Zhang
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Radiological and Ultrasound Technology ,Phantoms, Imaging ,Research Design ,Image Processing, Computer-Assisted ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Radiology, Nuclear Medicine and imaging ,Neural Networks, Computer ,Radiation Dosage ,Tomography, X-Ray Computed ,Algorithms - Abstract
Several reconstruction networks have been invented to solve the problem of learning-based computed tomography (CT) reconstruction. However, the application of neural networks to tomographic reconstruction remains challenging due to unacceptable memory space requirements. In this study, we present a novel lightweight block reconstruction network (LBRN), which transforms the reconstruction operator into a deep neural network by unrolling the filter back-projection (FBP) method. Specifically, the proposed network contains two main modules, which respectively correspond to the filter and back-projection of the FBP method. The first module of the LBRN decouples the relationship of the Radon transform between the reconstructed image and the projection data. Therefore, the following module, block back-projection, can use the block reconstruction strategy. Because each image block is only connected with part-filtered projection data, the network structure is greatly simplified and the parameters of the whole network are dramatically reduced. Moreover, this approach is trained end-to-end, working directly from raw projection data, and does not depend on any initial images. Five reconstruction experiments are conducted to evaluate the performance of the proposed LBRN: full angle, low-dose CT, region of interest, metal artifact reduction and a real data experiment. The results of the experiments show that the LBRN can be effectively introduced into the reconstruction process and has outstanding advantages in terms of different reconstruction problems.
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- 2022
29. Enhancing the X-Ray Differential Phase Contrast Image Quality With Deep Learning Technique
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Yongshuai Ge, Jinchuan Guo, Huitao Zhang, Peizhen Liu, Jianwei Chen, Yifan Ni, Zhicheng Li, Hairong Zheng, Ting Su, Jiecheng Yang, and Dong Liang
- Subjects
Noise measurement ,business.industry ,Computer science ,Image quality ,X-Rays ,0206 medical engineering ,Biomedical Engineering ,Image processing ,02 engineering and technology ,Grating ,Radiation ,Signal-To-Noise Ratio ,020601 biomedical engineering ,Convolutional neural network ,Signal ,Imaging phantom ,Radiography ,Signal-to-noise ratio ,Deep Learning ,Image noise ,Image Processing, Computer-Assisted ,Computer vision ,Artificial intelligence ,business ,Algorithms - Abstract
Objective: The purpose of this work is to investigate the feasibility of using deep convolutional neural network (CNN) to improve the image quality of a grating-based X-ray differential phase contrast imaging (XPCI) system. Methods: In this work, a novel deep CNN based phase signal extraction and image noise suppression algorithm (named as XP-NET) is developed. The numerical phase phantom, the ex vivo biological specimen and the ACR breast phantom are evaluated via the numerical simulations and experimental studies, separately. Moreover, images are also evaluated under different low radiation levels to verify its dose reduction capability. Results: Compared with the conventional analytical method, the novel XP-NET algorithm is able to reduce the bias of large DPC signals and hence increasing the DPC signal accuracy by more than 15%. Additionally, the XP-NET is able to reduce DPC image noise by about 50% for low dose DPC imaging tasks. Conclusion: This proposed novel end-to-end supervised XP-NET has a great potential to improve the DPC signal accuracy, reduce image noise, and preserve object details. Significance: We demonstrate that the deep CNN technique provides a promising approach to improve the grating-based XPCI performance and its dose efficiency in future biomedical applications.
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- 2020
30. Long noncoding RNA ZFAS1 silencing alleviates rheumatoid arthritis via blocking miR-296-5p-mediated down-regulation of MMP-15
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Ning Jia, Jing Zheng, Youyou Zhou, Huitao Zhang, Juan Liao, Peiying Zeng, Weiping Zhu, and Lin Lin
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0301 basic medicine ,Male ,Immunology ,Arthritis ,Down-Regulation ,Biology ,Cell Line ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,In vivo ,microRNA ,Databases, Genetic ,medicine ,Immunology and Allergy ,Gene silencing ,Animals ,Humans ,RNA, Small Interfering ,Pharmacology ,Gene knockdown ,Binding Sites ,Matrix Metalloproteinase 15 ,medicine.disease ,Arthritis, Experimental ,Synoviocytes ,In vitro ,Long non-coding RNA ,Mice, Inbred C57BL ,MicroRNAs ,030104 developmental biology ,RNAi Therapeutics ,030220 oncology & carcinogenesis ,Cancer research ,Disease Progression ,Joints ,RNA Interference ,RNA, Long Noncoding ,Signal Transduction - Abstract
Rheumatoid arthritis (RA), a chronic inflammatory disease, deprives patients' walking ability and reduces their life quality worldwide. Though recent studies have indicated the role of long noncoding RNA (lncRNA) ZFAS1 in several diseases, however, its role in RA remains uncharacterized. The present study aimed to unravel the the effect of ZFAS1 on RA. Herein, the RA mouse model and the human RA synoviocyte MH7A cell lines stimulated with TNF-α were established. ZFAS1 was next determined to be highly expressed in the mice with RA-like symptoms and TNF-α-stimulated MH7A cells while inhibiting ZFAS1 was demonstrated to promote proliferation and suppress apoptosis of MH7A cells. Furthermore, ZFAS1 knockdown exerted anti-inflammation effect in vitro and in vivo and reduced the arthritis index value. Moreover, RNA immunoprecipitation and dual-luciferase reporter assays identified the binding of ZFAS1 to microRNA (miR)-296-5p as well as the binding of miR-296-5p to matrix metalloproteinase-15 (MMP-15). Of note, ZFAS1 could bind miR-296-5p to up-regulate the expression of MMP-15. Our results from in vitro and in vivo experiments demonstrated silencing ZFAS1 mitigated RA-like symptoms such as inflammation and hyperplasia via miR-296-5p-dependent inhibition of MMP-15. Taken altogether, our study confirmed that ZFAS1 involved in RA progression by competitively binding to miR-296-5p and regulating MMP-15 expression.
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- 2020
31. Survey of Anxiety and Depression in Patients with Suspected and Confirmed Cases of COVID-19 During Hospitalization and Isolation
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Zhonghe Li, Yilan Chen, Juan Wang, Xiaohua Gong, Fei Li, Meifang Mai, Dongliang Xia, Huitao Zhang, and Xinming Guo
- Subjects
medicine.medical_specialty ,Coronavirus disease 2019 (COVID-19) ,Isolation (health care) ,business.industry ,Internal medicine ,medicine ,Anxiety ,In patient ,medicine.symptom ,business ,Depression (differential diagnoses) - Abstract
Objective: To observe the anxiety and depression experienced by patients with suspected and confirmed COVID-19 during hospitalization and isolation.Methods: A cross-sectional survey was performed with 66 patients with suspected and confirmed COVID-19 who were admitted to the Fifth Affiliated Hospital of Sun Yat-sen University from February 9, 2020, to February 22, 2020. The data collected including sex, age, education level, and nucleic acid test results. Anxiety, depression, and sleep disorders were evaluated using the Self-Rating Anxiety Scale (SAS), Self-Rating Depression Scale (SDS) and Pittsburgh Sleep Quality Index Scale (PSQI), respectively. Statistical analysis: SPSS 23.0 software was used for data processing, and multifactor logistic regression analysis was used to identify the independent risk factors. Spearman correlation analysis was used to study the correlations among the observed indicators; P Results: The incidences of anxiety, depression, and sleep disorders in the suspected case group were 18.2%, 18.2%, and 39.4%; the incidences of anxiety, depression, and sleep disorders in the diagnosed group were 42.4%, 57.6%, and 69.7%. The incidence of sleep disorders was higher than the domestic norm, and the difference was statistically significant (P P Conclusion: Patients with suspected and confirmed COVID-19, especially people aged 50 years and over, have significant anxiety, depression and sleep disorders. Anxiety, depression and sleep disorders in patients with confirmed cases are more serious than those in patients with suspected cases. It is necessary to focus on the psychological state of such patients, actively conduct psychological counseling, and reduce their anxiety and depression.
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- 2020
32. Dual phase grating based X-ray differential phase contrast imaging with source grating: theory and validation
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Jianwei Chen, Dong Liang, Kai Zhang, Peiping Zhu, Hairong Zheng, Yongshuai Ge, Jinchuan Guo, Shi Wei, Shiwo Deng, Huitao Zhang, and Jun Yang
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Diffraction ,Physics ,Geometrical optics ,business.industry ,Phase (waves) ,Phase-contrast imaging ,Physics::Optics ,02 engineering and technology ,Grating ,021001 nanoscience & nanotechnology ,Physical optics ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,010309 optics ,Interferometry ,Optics ,Thin lens ,0103 physical sciences ,0210 nano-technology ,business - Abstract
In this work, we developed a new theoretical framework using wave optics to explain the working mechanism of the grating based X-ray differential phase contrast imaging (XPCI) interferometer systems consist of more than one phase grating. Under the optical reversibility principle, the wave optics interpretation was simplified into the geometrical optics interpretation, in which the phase grating was treated as a thin lens. Moreover, it was derived that the period of an arrayed source, e.g., the period of a source grating, is always equal to the period of the diffraction fringe formed on the source plane. When a source grating is utilized, the theory indicated that it is better to keep the periods of the two phase gratings different to generate large period diffraction fringes. Experiments were performed to validate these theoretical findings.
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- 2020
33. A Small-Bodied Troodontid (Dinosauria, Theropoda) from the Upper Cretaceous Wulansuhai Formation of Inner Mongolia, China
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Josef Steigler, Qiyue Zhang, Qingwei Tan, Lin Tan, Huitao Zhang, and Shuo Wang
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Sister group ,Philovenator ,Sinovenator ,Anatomy ,Biology ,Body size ,Inner mongolia ,biology.organism_classification ,Theropoda ,Linhevenator ,Cretaceous - Abstract
A new small-bodied troodontid (LH PV39) recovered from the Upper Cretaceous Wulansuhai Formation, Suhongtu, Inner Mongolia, China, is described. The new specimen preserves six postaxial cervical vertebrae, five completely fused sacral and four posterior caudal vertebrae in addition to two manual unguals. The completely fused neurocentral junctions indicate that a skeletally mature individual of the same species of LH PV39 would be smaller than Philovenator and comparable in body size to a skeletal mature individual of Almas. The extremely dorsoventrally compressed sacral centra and neural canal, and the middle three sacral centra that are shorter and wider than the first and the last one distinguishing LH PV39 from other known troodontids. A series of phylogenetic analyses were conducted using modified published matrices. By coding LH PV39 in different strategies, the troodontid affinity of LH PV39 is confirmed and it was recovered as the sister taxon of either Mei and Sinovenator (LH PV39 scored as a separate OTU) or Linhevenator (incorporating LH PV39 into Philovenator) in the best resolved coelurosaurian interrelationships. The referral of LH PV39 to Philovenator does not seriously alter the phylogenetic position of Philovenator nor the interrelationships of troodontids. This new finding confirms that the small and large sized troodontids are coexisted in the Gobi Desert of the Mongolia Plateau until the end of Cretaceous.
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- 2020
34. Urinary 1-hydroxypyrene and smoking are determinants of LINE-1 and AhRR promoter methylation in coke oven workers
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Huitao Zhang, Wubin Wang, Hongjie Zhang, Yanfeng Fan, Yanli Liu, and Jin Yang
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Adult ,Male ,0301 basic medicine ,Coke oven ,Health, Toxicology and Mutagenesis ,Urinary system ,Aryl hydrocarbon receptor repressor ,complex mixtures ,Epigenesis, Genetic ,Water Purification ,Young Adult ,03 medical and health sciences ,Occupational Exposure ,Basic Helix-Loop-Helix Transcription Factors ,Genetics ,Humans ,Epigenetics ,Food science ,Promoter Regions, Genetic ,Coke ,Chromatography, High Pressure Liquid ,Carcinogen ,Pyrenes ,Chemistry ,Smoking ,technology, industry, and agriculture ,Sequence Analysis, DNA ,Methylation ,DNA Methylation ,Middle Aged ,Repressor Proteins ,Logistic Models ,Long Interspersed Nucleotide Elements ,030104 developmental biology ,Case-Control Studies ,DNA methylation ,Pyrosequencing - Abstract
Coke oven emissions (COE) containing polycyclic aromatic hydrocarbons (PAHs) are predominant toxic constituents of particulate air pollution that have been linked to increased risk of lung cancer. Aberrant DNA methylation is one of the best known epigenetic changes in human cancers and healthy subjects exposed to carcinogens. The purpose of this study is to explore the factors influencing the methylation of long interspersed nuclear element-1 (LINE-1) and aryl-hydrocarbon receptor repressor (AhRR) in coke oven workers. The study population is composed by coke oven workers (348) and water treatment workers (131). And their urinary PAH metabolites were analyzed by high performance liquid chromatography; DNA methylation were measured by pyrosequencing. The urinary PAHs metabolites were significantly elevated in coke oven workers (P 0.01). The results from multivariate logistic regression analysis showed that a high level of urinary 1-hydroxypyrene was associated with a significantly increased risk of hypomethylation of LINE-1 (OR: 1.80; 95% CI: 1.25, 2.60), and heavy smoking was associated with a significantly increased risk of hypomethylation of AhRR (OR: 1.44; 95% CI: 1.04, 2.00). Our findings demonstrate that urinary 1-hydroxypyrene may be a useful biomarker for evaluating the role of PAHs exposure on hypomethylation of LINE-1 among coke oven workers and that smoking may be an important factor affecting hypomethylation of AhRR.
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- 2018
35. Understanding working conditions for Entererococcus sp. L1 on methane production: A low temperature condition-based biogas production experiment
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Wang Xiaou, Yang Xinping, Aihemati Guli, Wang Zhifang, Huitao Zhang, Jingping Dai, Xie Yuqing, Feng Lei, Jiang Li, and Jing Chen
- Subjects
biology ,Strain (chemistry) ,Renewable Energy, Sustainability and the Environment ,Chemistry ,Forestry ,biology.organism_classification ,chemistry.chemical_compound ,Biogas ,Fermentation ,Food science ,Methane production ,Citric acid ,Waste Management and Disposal ,Agronomy and Crop Science ,Cow dung ,Bacteria ,Biogas production - Abstract
To provide an elaborate understanding on the performance of Entererococcus sp. L1 in biogas production at different fermentation periods under controlled low temperature, biogas optimization experiments was undertaken. Specifically, microscopic and culture techniques, physiological and biochemical studies, and measurement of optical densities at 600 wavelengths (OD600) were undertaken to identify and characterize the Entererococcus sp. L1. The aim was to identify the strain and determine its optimum working conditions in biogas production at different fermentation periods under controlled low temperature. The result confirmed the strain as hydrogen-producing bacteria (Enteroccus sp. L1). It was also confirmed that the organic nitrogen source was significantly better than the inorganic nitrogen source in supporting the growth of the bacteria. The optimal pH and temperature for the growth and biogas production performance of the Entererococcus sp. L1 was 7 and 15 °C respectively. The Na2HPO4 concentration of 0.2% was confirmed to be best for the optimum growth of the bacteria, and addition of citric acid resulted to significant inhibitory effect on the growth of the Entererococcus sp. L1. The effect of adding different concentrations (0, 1%, 5%, 10%) of the L1 bacteria inoculum on cow dung at simulated low temperature (15 °C) biogas fermentation showed that 10% treatment had the highest gas production of 3121 ml, which was 89% higher than the control (0 concentration). The daily gas production rate was 0.02 m3/(m−3d−1), which was 90% higher than the control. This showed that 10% treatment provided the best gas production result, with highest gas production occurring at 10% treatment within 31–60 days of fermentation (p
- Published
- 2021
36. Smoking modify the effects of polycyclic aromatic hydrocarbons exposure on oxidative damage to DNA in coke oven workers
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Hongjie Zhang, Wubin Wang, Yanfeng Fan, Huitao Zhang, Jin Yang, and Yanli Liu
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Adult ,Male ,0301 basic medicine ,China ,Coke oven ,Urinary system ,Air Pollutants, Occupational ,Patient Health Questionnaire ,010501 environmental sciences ,complex mixtures ,01 natural sciences ,Oxidative damage ,Young Adult ,03 medical and health sciences ,chemistry.chemical_compound ,Age Distribution ,Risk Factors ,Occupational Exposure ,medicine ,Humans ,Food science ,Polycyclic Aromatic Hydrocarbons ,Lung cancer ,Coke ,Carcinogen ,0105 earth and related environmental sciences ,Guanosine ,Heavy smoking ,Smoking ,Public Health, Environmental and Occupational Health ,Middle Aged ,medicine.disease ,Oxidative Stress ,Logistic Models ,030104 developmental biology ,chemistry ,Air Pollution, Indoor ,Environmental chemistry ,Population study ,Female ,DNA ,DNA Damage - Abstract
Coke oven emissions containing polycyclic aromatic hydrocarbons (PAHs) are predominant toxic constituents of particulate air pollution that have been linked to increased risk of lung cancer. Numerous epidemiological studies have suggested that oxidative DNA damage may play a pivotal role in the carcinogenic mechanism of lung cancer. Little is known about the effect of interaction between PAHs exposure and lifestyle on DNA oxidative damage. The study population is composed by coke oven workers (365) and water treatment workers (144), and their urinary levels of four PAH metabolites and 8-hydroxydeoxyguanosine (8-OHdG) were determined. Airborne samples of exposed sites (4) and control sites (3) were collected, and eight carcinogenic PAHs were detected by high-performance liquid chromatography. The median values of the sum of eight carcinogenic PAHs and BaP in exposed sites were significantly higher than control sites (P
- Published
- 2017
37. Additional file 1: of CYP1A1 methylation mediates the effect of smoking and occupational polycyclic aromatic hydrocarbons co-exposure on oxidative DNA damage among Chinese coke-oven workers
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Yanli Liu, Xuejing Li, Zhang, Bin, Fu, Ye, Aimin Yang, Hongjie Zhang, Huitao Zhang, Yingying Niu, Jisheng Nie, and Yang, Jin
- Abstract
Table S1. Primers used for pyrosequencing size of the PCR amplicons and position of the primers from the transcription start point. Table S2. Distributions of urine PAH metabolites among 500 occupational workers. Table S3. The correlation coefficients (rs) of urine PAH metabolites among 500 occupational workers. Table S4. The correlation coefficients (rs) of CYP1A1 methylation among 500 occupational workers. Table S5. Contribution rates of smoking on urine PAH metabolites among 500 occupational workers. (DOC 67 kb)
- Published
- 2019
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38. Ubiquitin Protein Ligase Ring2 Is Involved in S-phase Checkpoint and DNA Damage in Cells Exposed to Benzo[a]pyrene
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Wentao Chen, Yanfeng Fan, Hongjie Zhang, Wubin Wang, Jin Yang, and Huitao Zhang
- Subjects
0301 basic medicine ,Small interfering RNA ,biology ,DNA damage ,Health, Toxicology and Mutagenesis ,General Medicine ,Cell cycle ,Toxicology ,Biochemistry ,Molecular biology ,Proliferating cell nuclear antigen ,Ubiquitin ligase ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,chemistry ,Benzo(a)pyrene ,030220 oncology & carcinogenesis ,polycyclic compounds ,biology.protein ,Molecular Medicine ,Molecular Biology ,DNA ,Nucleotide excision repair - Abstract
Previous studies in our laboratory demonstrated that Ring2 may affect DNA damage and repair through pathways other than through regulating the expression of the nucleotide excision repair protein. In a series of experiments using wild-type cell (16HBE and WI38) and small interfering RNA (siRNA) Ring2 cells exposed to benzo[a]pyrene (BaP), we evaluated the cell cycle and DNA damage. The benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE–DNA) adduct assay demonstrated that in vitro exposure to BaP increased DNA damage in a time- and dose-dependent manner in wild-type and siRNA Ring2 cells. Analysis of covariance showed that a decrease of Ring2 caused DNA hypersensitivity to BaP. Flow cytometry results and proliferating cell nuclear antigen levels indicated that inhibition of Ring2 attenuated the effect of BaP on S-phase arrest. Taken together, these data implied that the lower proportion of cells in the S phase induced by inhibition of Ring2 may play an important role in DNA hypersensitivity to BaP.
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- 2016
39. 3D Hessian deconvolution of thick light-sheet z-stacks for high-contrast and high-SNR volumetric imaging
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Ruyi Zheng, Ke Du, Liangyi Chen, Hongrun Yang, Zhe Zhang, Huitao Zhang, Dongzhou Gou, Fan Feng, Zhang Guangyi, Louis Tao, and Heng Mao
- Subjects
Hessian matrix ,Physics ,Deblurring ,Pixel ,Optical sectioning ,business.industry ,Image processing ,02 engineering and technology ,Iterative reconstruction ,021001 nanoscience & nanotechnology ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,010309 optics ,symbols.namesake ,Optics ,0103 physical sciences ,symbols ,Depth of field ,Deconvolution ,0210 nano-technology ,business - Abstract
Due to its ability of optical sectioning and low phototoxicity, z -stacking light-sheet microscopy has been the tool of choice for in vivo imaging of the zebrafish brain. To image the zebrafish brain with a large field of view, the thickness of the Gaussian beam inevitably becomes several times greater than the system depth of field (DOF), where the fluorescence distributions outside the DOF will also be collected, blurring the image. In this paper, we propose a 3D deblurring method, aiming to redistribute the measured intensity of each pixel in a light-sheet image to in situ voxels by 3D deconvolution. By introducing a Hessian regularization term to maintain the continuity of the neuron distribution and using a modified stripe-removal algorithm, the reconstructed z -stack images exhibit high contrast and a high signal-to-noise ratio. These performance characteristics can facilitate subsequent processing, such as 3D neuron registration, segmentation, and recognition.
- Published
- 2020
40. Higher Incidence of Renal Allograft Glomerulonephritis in Living-Related Donor Kidney Transplantation
- Author
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Long-shan Liu, Su-xiong Deng, C. Wang, Qing-Ling Fu, Y. Dai, Huitao Zhang, Yunyi Xiong, J. Li, and Rongrong Deng
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Adult ,Male ,medicine.medical_specialty ,Time Factors ,030232 urology & nephrology ,Urology ,030204 cardiovascular system & hematology ,Organ transplantation ,Nephropathy ,Diabetic nephropathy ,03 medical and health sciences ,0302 clinical medicine ,Glomerulonephritis ,Risk Factors ,Living Donors ,Medicine ,Humans ,Transplantation, Homologous ,Kidney transplantation ,Proportional Hazards Models ,Retrospective Studies ,Transplantation ,business.industry ,Incidence (epidemiology) ,Incidence ,Graft Survival ,Glomerulonephritis, IGA ,Middle Aged ,medicine.disease ,Kidney Transplantation ,Obstructive Nephropathy ,surgical procedures, operative ,Surgery ,Female ,business - Abstract
Glomerulonephritis recurrence has emerged as one of the leading causes of allograft loss. We aimed to investigate the effect of living-related and deceased donation on the incidence of renal allograft glomerulonephritis and its effect on renal allograft survival.Adult renal allograft recipients with primary glomerulonephritis were enrolled. Transplantation date was from Feb 2004 to Dec 2015. Exclusion criteria included combined organ transplantation, structural abnormality, diabetic nephropathy, hypertension nephropathy, obstructive nephropathy, and primary uric acid nephropathy. The incidence of biopsy-proven allograft glomerulonephritis was compared between the living-related donor group and the deceased donor group. Graft survival was assessed with Kaplan-Meier method, and Cox proportional hazard model was used to evaluate the effect of posttransplant glomerulonephritis on graft outcome.There were 525 living-related donor kidney transplant recipients (LRKTx) and 456 deceased donor kidney transplant recipients (DDKTx) enrolled. The incidence of IgA nephropathy was 8.8% in the LRKTx group and 1.3% in the DDKTx group (P .001); the incidence of focal segmental glomerulosclerosis (FSGS) was 3.8% in the LRKTx group and 1.5% in the DDKTx group (P = .03). FSGS increased the risk of graft failure compared with non-FSGS (hazard ratio [HR], 3.703 [1.459-9.397]; P = .006). IgA nephropathy increased the risk of graft failure by over 5 times 5 years after kidney transplantation compared with non-IgA nephropathy, but it did not affect early allograft survival (HR for ≥5 years, 6.139; 95% CI, 1.766-21.345; P = .004; HR for 5 years, 0.385 [0.053-2.814]; P = .35).Higher incidence of IgA nephropathy and FSGS in renal allograft was observed in living-related donor kidney transplantation compared with deceased donor kidney transplantation. De novo or recurrent IgA nephropathy and FSGS impaired long-term renal allograft survival.
- Published
- 2018
41. A large enantiornithine bird from the Lower Cretaceous of China and its implication for lung ventilation
- Author
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Defeng Chen, Zihui Zhang, Lianhai Hou, and Huitao Zhang
- Subjects
Rib cage ,biology ,Enantiornithes ,Jiufotang Formation ,Anatomy ,musculoskeletal system ,biology.organism_classification ,Cretaceous ,Time of death ,medicine.anatomical_structure ,Thoracic vertebrae ,medicine ,Mesozoic ,Lung ventilation ,Ecology, Evolution, Behavior and Systematics - Abstract
The Enantiornithes were the most taxonomically diverse bird group in the Mesozoic. Most of the known taxa are from Lower Cretaceous deposits of the Jehol Group in north-eastern China. A new specimen from the Jiufotang Formation in Jianchang, Liaoning Province, is described here; being a subadult individual at the time of death it had reached a relatively large size. The presence of uncinate processes, bicapitate and forked vertebral ribs, sternal ribs that were all of similar length, as well as the location of parapophyses and diapophyses on the thoracic vertebrae, may imply a rigid and volume-constant lung, and less efficient lung ventilation in enantiornithines. © 2014 The Linnean Society of London, Biological Journal of the Linnean Society, 2014, 113, 820–827. ADDITIONAL KEYWORDS: Jianchang – Jiufotang Formation – Liaoning province – respiratory apparatus – ribs – thoracic vertebrae.
- Published
- 2014
42. Ubiquitin Protein Ligase Ring2 Is Involved in S-phase Checkpoint and DNA Damage in Cells Exposed to Benzo[a]pyrene
- Author
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Jin, Yang, Wentao, Chen, Yanfeng, Fan, Huitao, Zhang, Wubin, Wang, and Hongjie, Zhang
- Subjects
Dose-Response Relationship, Drug ,Tumor Suppressor Proteins ,7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide ,Bronchi ,Epithelial Cells ,Fibroblasts ,Cell Line ,DNA Adducts ,Gene Expression Regulation ,Proliferating Cell Nuclear Antigen ,S Phase Cell Cycle Checkpoints ,Benzo(a)pyrene ,Humans ,RNA, Small Interfering ,Ubiquitin Thiolesterase ,Cell Proliferation ,DNA Damage ,Signal Transduction - Abstract
Previous studies in our laboratory demonstrated that Ring2 may affect DNA damage and repair through pathways other than through regulating the expression of the nucleotide excision repair protein. In a series of experiments using wild-type cell (16HBE and WI38) and small interfering RNA (siRNA) Ring2 cells exposed to benzo[a]pyrene (BaP), we evaluated the cell cycle and DNA damage. The benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE-DNA) adduct assay demonstrated that in vitro exposure to BaP increased DNA damage in a time- and dose-dependent manner in wild-type and siRNA Ring2 cells. Analysis of covariance showed that a decrease of Ring2 caused DNA hypersensitivity to BaP. Flow cytometry results and proliferating cell nuclear antigen levels indicated that inhibition of Ring2 attenuated the effect of BaP on S-phase arrest. Taken together, these data implied that the lower proportion of cells in the S phase induced by inhibition of Ring2 may play an important role in DNA hypersensitivity to BaP.
- Published
- 2016
43. Quantitative coherence analysis with an X-ray Talbot–Lau interferometer
- Author
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Peiping Zhu, Wanxia Huang, Ziyu Wu, Qingxi Yuan, Youli Hong, Xiaosong Liu, Kai Zhang, Kun Gao, Xin Ge, Zhili Wang, and Huitao Zhang
- Subjects
Physics ,Interferometric visibility ,business.industry ,Astrophysics::High Energy Astrophysical Phenomena ,Physics::Optics ,Degree of coherence ,Grating ,Biochemistry ,Analytical Chemistry ,Generator (circuit theory) ,Interferometry ,Optics ,Point (geometry) ,Physics::Atomic Physics ,Absorption (electromagnetic radiation) ,business ,Visibility - Abstract
Differential phase-contrast (DPC) X-ray imaging has been performed in the Talbot-Lau configuration, in which the X-ray source was a combination of an absorption grating and a laboratory X-ray generator. We report here quantitative analysis of partial coherence effects on the X-ray Talbot-Lau interferometer. Based on the visibility of the self-image, the well-known geometry condition is reproduced. It is shown that effects of partial coherence are determined by the opening ratio of the source grating, and that the effects are independent of the Talbot order and the type of the phase grating, a condition quite different from those in a Talbot interferometer. A possible explanation is discussed from the point of view of the effective spatial coherence length. Taking into account the available X-ray flux and experimental fluctuations, we present the optimum opening ratio. Furthermore, we mention that our results can also be successfully used to discuss the properties of a multiline X-ray source.
- Published
- 2010
44. Studies of Magnetic Fluid Plane Sealing in Split Sealing Device
- Author
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Huitao Zhang
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Materials science ,Plane (geometry) ,Applied Mathematics ,Mechanical Engineering ,Geometry ,Computer Science Applications - Published
- 2018
45. BPF-based reconstruction algorithm for multiple rotation–translation scan mode
- Author
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Peng Zhang, Ming Chen, and Huitao Zhang
- Subjects
business.industry ,Process (computing) ,Reconstruction algorithm ,Industrial computed tomography ,Translation (geometry) ,Feature (computer vision) ,General Materials Science ,Computer vision ,Artificial intelligence ,business ,General ,Rotation (mathematics) ,Image resolution ,Interpolation ,Mathematics - Abstract
In industrial CT, it is often required to inspect large objects using short line-detectors. To acquire the complete CT data for the scanning slice of large objects using short line-detectors, some multi-scan modes have been developed. But the existing methods reconstructing an image from the data scanned by multi-scan modes have to rebin the data into fan-beam or parallel-beam form via data interpolation. The data rebinning process not only increases great computational cost, but also degrades image resolution. In this paper, we propose a backprojection-filtration (BPF)-based reconstruction algorithm for rotation–translation (RT) multi-scan mode. An important feature of the proposed algorithm is that data rebinning process is not introduced. The simulation results have verified the validity of the proposed algorithm.
- Published
- 2008
- Full Text
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46. Bovine parathyroid hormone enhances osteoclast bone resorption by modulating V-ATPase through PTH1R
- Author
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Zhuo Li, Xinling Liang, Bin Zhang, Li Zhang, Jianchao Ma, Huitao Zhang, Zhonghe Li, Weiping Zhu, Wei Shi, Shuangxin Liu, and Sijia Li
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Vacuolar Proton-Translocating ATPases ,Parathyroid hormone ,Osteoclasts ,Biology ,vacuolar-type H+ adenosine triphosphatase ,Bone resorption ,Bone remodeling ,03 medical and health sciences ,Mice ,Western blot ,Osteoclast ,Bone Marrow ,Internal medicine ,Genetics ,medicine ,V-ATPase ,Animals ,Humans ,Bone Resorption ,Receptor ,Receptor, Parathyroid Hormone, Type 1 ,medicine.diagnostic_test ,General Medicine ,Articles ,Resorption ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Gene Expression Regulation ,Parathyroid Hormone ,proton pump ,osteoclast ,Cattle ,Bone Remodeling ,Macrolides - Abstract
The vacuolar-type H+ adenosine triphosphatase (V-ATPase) plays an important role in cellular acidification and bone resorption by osteoclasts. However, the direct effect of bovine parathyroid hormone (bPTH) on V-ATPase has not yet been elucidated. The aim of the present study was to assess the effects of bPTH on V-ATPase and osteoclasts. Osteoclasts from bone marrow (BM)-derived monocytes of C57BL/6 mice were cultured with or without bPTH. The mRNA and protein expression levels of the V-ATPase a3-subunit and d2-subunit (by RT-qPCR and western blot analysis), V-ATPase activity (using the V type ATPase Activity Assay kit) and the bone resorption function of osteoclasts (by bone resorption assay) were examined following treatment with various concentrations of bPTH (0.1, 1.0, 10 and 100 ng/ml) alone or with bPTH and its inhibitor, bafilomycin A1. Furthermore, the expression of parathyroid hormone (PTH) receptors in osteoclasts was also detected. The results revealed that the mRNA and protein expression levels of V-ATPase a3-subunit and d2-subunit increased in a dose‑dependent manner, paralleling the level of bPTH present. In addition, an increase in the concentration of bPTH was accompanied by the increased resorption capability of osteoclasts, whereas bone resorption was inhibited in the presence of bafilomycin A1. In addition, we confirmed the existence of parathyroid hormone 1 receptor (PTH1R) in osteoclasts using three different methods (RT-qPCR, western blot analysis and immunofluorescence staining). We found that bPTH enhanced the bone resorption capability of osteoclasts by modulating the expression of V-ATPase subunits, intracellular acidification and V-ATPase activity. Thus, we propose that PTH has a direct effect on osteoblasts and osteoclasts, and that this effect is mediated through PTH1R, thus contributing to bone remodeling.
- Published
- 2015
47. Expression and clinical evidence of miR-494 and PTEN in non-small cell lung cancer
- Author
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Nan Chen, Juan Wang, Yi-feng Liao, Huitao Zhang, Hongyu Zhang, Hongtao Chen, and Tianze Liu
- Subjects
Oncology ,Adult ,Male ,medicine.medical_specialty ,In situ hybridization ,Kaplan-Meier Estimate ,Biology ,Malignancy ,Disease-Free Survival ,Internal medicine ,Carcinoma, Non-Small-Cell Lung ,medicine ,Carcinoma ,Biomarkers, Tumor ,Tensin ,PTEN ,Humans ,Clinical significance ,Lung cancer ,In Situ Hybridization ,Aged ,Neoplasm Staging ,Aged, 80 and over ,PTEN Phosphohydrolase ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Lymphatic Metastasis ,biology.protein ,Immunohistochemistry ,Female - Abstract
The aim of this study was to explore the expression and clinical significance of miR-494 and PTEN (phosphatase and tensin homologue deleted on chromosome ten) in non-small cell lung cancer (NSCLC). Immunohistochemistry for PTEN and in situ hybridization (ISH) for miR-494 were performed in 92 NSCLC tissues and 10 normal lung tissues to detect their expression, and correlation between their expression with clinical characteristics and prognosis was analyzed. The expression of miR-494 was significantly higher in NSCLC than in normal lung tissues (P = 0.004). The positive expression of PTEN protein in the lung carcinoma tissues was significantly lower than that in the normal lung tissues (P = 0.013), while the level of miR-494 expression was negatively correlated with PTEN expression (r = −0.577, P
- Published
- 2014
48. Design and Implementation of Digital Campus Platform
- Author
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Lijiuan Zhang, Bo Feng, and Huitao Zhang
- Subjects
Scheme (programming language) ,business.industry ,Process (engineering) ,Computer science ,Information technology ,Investment (macroeconomics) ,Service process ,Engineering management ,Management information systems ,Information system ,business ,computer ,ComputingMilieux_MISCELLANEOUS ,computer.programming_language - Abstract
The digital campus is the use of methods and tools of information technology, campus resources, management and service process of digital campus, forming the digital environment, make the reality of campus environment with information system in time and space to be extended. This paper from the digitized campus overall scheme design were analyzed, the information in the data, process, investment and other aspects of integration of design, focusing on various types of management information system and all kinds of basic digital campus platform integration, and gives the corresponding integrated example.
- Published
- 2013
49. XQPage: A Webpage Programming Language Based on XQuery
- Author
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Huitao Zhang, Husheng Liao, and Zhiming Gui
- Subjects
computer.internet_protocol ,Programming language ,Computer science ,Event (computing) ,Context (language use) ,Construct (python library) ,computer.software_genre ,Query language ,Electronic mail ,XQuery ,ComputingMethodologies_DOCUMENTANDTEXTPROCESSING ,Document Object Model ,computer ,XML ,computer.programming_language - Abstract
The paper proposes a new webpage programming language that is based on XQuery: XQPage. It augments the abilities of XQuery to support the Document Object Model in browser, and to support the event response mechanism of DOM objects through extending the updating facility of XQuery. In contrast to the traditional ways using XQuery in webpage currently, it does not need any host language and can be directly applied in webpage design. It enables developers to construct, modify and operate webpage in native XML processing language. The demonstration illustrates that it is flexible, efficient, easy to use and can meet the requirement of most webpage programming.
- Published
- 2009
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