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Copyright of Van Health Sciences Journal / Van Sağlık Bilimleri Dergisi is the property of Van Yuzuncu Yil University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Turkiye Klinikleri Journal of Nursing Sciences is the property of Turkiye Klinikleri and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Kolon kanseri, tüm dünyada yaygın olan ve yüksek oranda ölümcül bir kanser tipidir. Tüm kanserlerde olduğu gibi kolon kanserinin seyrinin izlenmesi kritik bir öneme sahiptir. 5-Florourasil (5-FU) kolon kanserlerinde sıklıkla kullanılan bir antikanser ilaçtır ve hücre ölümü esnasında hücrelerde bir takım biyokimyasal ve moleküler farklılıklara neden olmaktadır. Bu çalışmada HCT116 ve HT29 kolon kanseri hücreleri 5-FU kemoterapi ilacı ile inkübe edildikten sonra, 5FU’nun hücreler üzerinde oluşturduğu biyokimyasal değişikliğin tespiti için Fourier transform kızılötesi (FTIR) spektroskopisi kullanılmıştır. 5-FU'nun inhibisyon yüzdesinin 50'ye eşit olduğu konsantrasyonu (IC50), MTT yöntemi kullanılarak belirlendi. IC50 değeri saptandıktan sonra FTIR ölçümleri alındı. Buna göre, 5-FU IC50 değeri sırasıyla HCT116 hücreleri için 12,69 µg/ml, HT29 hücreleri için 10,10 µg/ml bulunmuştur. 5-FU’nun sebep olduğu hücre ölümünün göstergeleri olduğu söylenen lipit içeriğindeki artış ile nükleik asit oranı, total hücresel proteinlerin α-sarmal ve β-yaprak ikincil yapıları ve aminoasit kalıntılarının azalması, kollajen konformasyonundaki değişiklikler gibi IR biyobelirteçlerindeki değişiklikler FTIR spektroskopisi ile gösterilmiştir. Sonuç olarak hem ucuz ve hem de hızlı bir yöntem olan FTIR ile kanser hücre ölümünün incelenmesi alternatif bir yöntem olarak kullanılabilir.

Objective: Colon cancer is a chronic fatal disease that severely affects the quality of life of patients and is associated with various complications. Vitamin B complex shows positive anti-inflammatory effects when taken as a food supplement. The aim of this study was to investigate the anti-cancer effect of vitamin B complex with liposomes in colon cancers (HCT-116 and HT-29 cell lines) using photodynamic therapy. Material-Method: Vitamin B complex, which is the first step of the study, was encapsulated with liposome. Vitamin B complex with liposome was characterized by Zetasizer device. The MTT (3-4,5-dimethyl-thiazolyl-2,5-diphenyltetrazolium bromide) assay was used to determine the % survival rates in HCT-116 and HT-29 cell lines. The same cell lines were also analyzed by flow cytometry to show which death pathways were selected by the vitamin B complex with the effect of photodynamic therapy. Results: It was found that the dimensions of the vitamin B complex with liposomes varied between 100 nm and 200 nm. Liposomal vitamin B complex (photosensitizer) at 7.5 J/cm2 light dose at 0.25 mg/mL in the dark experiment, while the percentage of HCT-116 cell viability was 75%, this rate decreased to 47% after the light experiment (****p <0.001). While the percentage of HT-29 cell viability was 70% in the dark test for 0.5 mg/mL vitamin B complex solution, this rate decreased to 35% after the light test (****p<0.001). The desired photodynamic efficiency is closer to these results. In the cell death pathway assay, the concentration of liposomal vitamin B complex was applied to HCT-116 and HT-29 cells at 1 mg/mL. While 40% apoptosis was observed in the HCT-116 cell line, 60% apoptosis was observed in the HT-29 cell line. Conclusion: Repositioning of marketed drugs has emerged as a new approach to the discovery of new antitumor drugs. The development of new uses for marketed drugs has proven to be an efficient and rapid way to develop new anti-tumor drugs. Our data suggest that the in vitro use of vitamin B complex with photodynamic therapy is promising in some types of colorectal cancer. [ABSTRACT FROM AUTHOR]

High IL-6 levels are associated with aggressive tumor growth and response to therapy in many types of cancer. Patients with high circulating IL-6 levels are generally associated with poorer prognosis and shorter survival, while a lower IL-6 level is associated with better response to treatment. Studies conducted in recent years emphasize that colorectal cancer (CRC) is regulated by IL-6, serum IL-6 level is increased in CRC patients and this increase is correlated with tumor size. Having hydratase activity, which plays an important role in maintaining the proper acid-base balance in organisms, CA3 also protects cells from damage caused by oxidative stress by destroying reactive oxygen radicals, and also facilitates the rapid conversion of glycolytic intermediates oxaloacetate and citrate, enabling them to participate in fatty acid metabolism. Recent studies have focused on the relationship between CAIII and cancer. It has been determined that CAIII increases the invasion of cells and the expression of CAIII is significantly decreased in Liver carcinoma (HCC). It has also been determined that CAIII expression is suppressed through MEK-1 and PI3K pathways in colon cancer cell line HT-29 cells. Finally, it has been shown that HIF1-α, the regulatory protein of the hypoxic pathway in prostate cancer, directly binds to the CAIII promoter. In our study, the regulation of IL-6 cytokine on the CAIII gene was evaluated in 2 different steps. The effect of CAIII mRNA levels and protein levels were determined by Real Time PCR and Western blot analysis, respectively. The sample in which total RNAs and protein obtained from HT-29 cells treated with 500 U of IL-6 cytokine were used for both analysis. It was determined that the expression of CAIII at both mRNA and protein levels were decreased by the effective regulation of IL-6.In conclusion, this study elucidates the regulatory effect of IL-6 cytokine on cancer-related protein CA3 gene regulation. [ABSTRACT FROM AUTHOR]

Amaç : Kolorektal kanserlede ( KRK) venöz invazyonun(Vİ) prognostik önemi uzun yıllardır bilinmektedir. Vİ uzak organ metastazları için bağımsız prediktör olarak kabul edilmektedir. KRK'lerde beklenen Vİ oranı en az % 25’dir. Gastrointestinal kanal spesmenlerine spesifik bakan patologların olduğu merkezlerde bu oran genel patalogların baktığı spesmenlerle karşılaştırıldığında daha fazladır. Elastik Lif Boyası (ELB) kullanımının VI oranlarını artırdığı ileri sürülmektedir. Bu çalışmada amacımız Vİ olmayan KRK olgularına ELB uygulayarak yeniden değerlendirmek, yanlış yorumlamaya neden olan etkenleri belirlemek ve ELB'nin rutinde kullanımını sorgulamaktır. Gereç ve Yöntemler: 2018-2020 tarihleri arasında tanı almış 166 KRK materyalinden Vİ bulunmayan, Vİ belirlenirken boyama işlemi yapılmamış, 77 olgunun preparatları yeniden incelendi. Seçilen bir bloğa ELB uygulandıktan sonra yeniden değerlendirildi. Bulgular: Olguların 49’u Erkek (%63,6), 28’i Kadın (%36,4), E/K oranı: 1,75 idi. Yaş ortalaması 63,5 (29-101) yaş idi. Ortalama tümör çapı 4,85 cm (1-11 cm) idi. Olguların 6’sı (%7.8) high grade, 71’i (%92.2) low grade histolojik dereceye sahipti. Olguların patolojik T evreleri sırasıyla, 3 olgu pT1 (%3,9), 14 olgu pT2 (%18,2), 50 olgu pT3 (%65), 10 olgu pT4 (%12,9) idi. Patolojik N evreleri sırasıyla, 2 olgu pNx (%2,6), 57 olgu pN0 (%74), 14 olgu pN1 (%18,2), 4 olgu pN2 (%5,2) idi. ELB yapılan preparatlar yeniden değerlendirildi. 77 olgunun 6’sında (%7,8) ELB boyama sonrası Vİ tespit edildi. Sonuç: Çalışmamızda ELB ile Vİ tespit edilen olgularda, oblitere olmuş damar duvarlarında tümörü çevreleyen alanın desmoplastik stroma lehine değerlendirildiğini gözlemledik. Sonuç olarak H E kesitlerde Vİ bulunmayan olguların tamamına ELB gibi damar duvarı belirleyicilerinin uygulanmasını önermekteyiz. Böylece hastanın tedavi protokolünü ve sağ kalımını etkileyen Vİ’nin yorumlanmasında oluşabilecek hatalar azaltılmış olacaktır.

Copyright of Duzce University Journal of Science & Technology is the property of Duzce University Journal of Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: Laparoscopic colorectal surgery has become a widely used method in many centers today. In our study, it was aimed to compare the postoperative clinical and oncological results in patients who underwent laparoscopic and open colorectal surgery and to compare them with the literature. Material and Method: The data of patients who underwent colorectal surgery between January 2012 and December 2018 at the Dışkapı Yıldırım Beyazıt Training and Research Hospital General Surgery clinic were analyzed retrospectively. Results: A total of 544 patients were included in our study, of which 438 (80.5%) underwent open resection, 73 (13.4%) laparoscopic, 33 (6.1%) underwent resection with conversion. In the series, the number of cases that were operated urgently was 158 (29.0%), and the number of elective cases was 386 (71.0%). A total of 156 (28.7%) postoperative complications were detected. When evaluated according to the groups; There were 20 (27.4%) postoperative complications in the laparoscopic surgery group, 126 (28.8%) in the open surgery group, and 10 (30.3%) in the conversion group. The mean number of lymph nodes removed in rectal cancers is 19.0±9.7 in the open procedure and 22.3±11.6 in the laparoscopic procedure (p: 0.198). A positive circumferential surgical margin was found in 1 (2.3%) and 8 (5.9%) patients with rectal cancer in the laparoscopic and open surgery groups, respectively (p: 0.456). The mean distal surgical margin was 45.1±39.8 mm in the open group and 41.1±37.7 mm in the laparoscopic group (p: 0.608). Conclusion: Laparoscopic colorectal cancer surgery has comparable clinical and oncological outcomes to the open procedure. Given the many known advantages of minimally invasive surgery, laparoscopic surgery will likely become the standard treatment for colon cancer. [ABSTRACT FROM AUTHOR]

Aim:Liquid biopsy-based measurement of post-translational histone modifications (PTHMs) in bodily fluids of patients with cancer represents a novel area of biomarker research. Here, we tested the applicability of an approach combining enzyme-linked immunosorbent assays (ELISA)-like measurement of histone methylation and quantitative polymerase chain reaction to extract and quantify pericentric histone 4 lysine 20 trimethylation (H4K20me3)-related circulating DNA.Materials and Methods:After adding plasma into ELISA wells and letting H4K20me3-related nucleosomes bind to the antibody, nucleosomal DNA was detached from immune complexes using a buffer including NaHCO3, SDS, and proteinase K and purified. A single copy- and a multiple copy-sequence from pericentric regions of chromosomes 16 and 1 were amplified to quantify H4K20me3-related DNA from patients with colon cancer or colonic polyps.Results:Relative plasma level of H4K20me3-related nucleosomal DNA was lower in the patients with colon cancer than in controls for both target sequences, with a statistically significant difference at the chr16-specific target (relative values were 0.037 vs. 0.073, respectively; p=0.008).Conclusion:That H4K20me3-related nucleosomal DNA is present in decreased levels in colon cancer confirms our previous findings attained using other techniques. In conclusion, our findings indicate the applicability of direct extraction of protein-bound DNA from ELISA immune complexes and it could provide a surrogate means in the assessment of PTHMs or other protein-DNA interactions.

Objective: Anti-cancer drug research plays an important role for chemotherapeutic treatments in various types of cancer. Pyrolidine derived compounds have been reported by many researchers to be a potent anti-cancer compound. It is aimed to investigate the effects of pyrolidine-derived compounds that are thought to be new drug candidates with antiproliferative activity on DLD-1 and CCD-18CO cell lines. Material and Methods: The antiproliferative activity of the pyrrolidine-derived compound was determined for 24 hours at different concentrations (25-100 µM) on DLD-1 (human colon cancer) and CCD-18CO (normal colon fibroblast) cell lines by comparing MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5 Diphenyltetrazolium bromide) and RTCA (real-time cell analysis) assays. The significance of the differences between data sets in the MTT assay was analyzed statistically by ANOVA with SPSS 20.0 program for DLD-1 and CCD-18CO cell lines. Results: It has been determined that pyrrolidine-derived compounds reduce the number of DLD-1 cancer cells according to negative control with the MTT method and suppress the DLD-1 cell according to the RTCA assay results. Thus, the compounds have been shown to inhibit cell proliferation and have antiproliferative activity. Conclusion: Pyrrolidine-derived compounds will be the first step for antiproliferative activity studies in DLD-1 cancer cells and will guide the next studies.

Berry fruits and their products are known as “functional foods” and have a growing interest in the food industry. Berries have high antioxidant capacity and anthocyanin contents in comparison to other fruits, and they are considered to have protective effects on human health by reducing the risks and symptoms of diseases. Although Turkey is in a prominent position with fruit production, having in the fifth place in the world according to the 2019 Turkey Statistical Institute (TUIK) data, the amount of berry fruit production is low, and its importance is still poorly understood. For this reason, it is very important to increase the interest in berry fruits, which are thought to have a positive effect on health, in our country, both scientifically and economically in order to increase their production. This review aims to evaluate the bioactive compounds of berry fruits and their health effects on human with clinical studies conducted in the last decade. To reach this goal, a literature search was conducted in DergiPark, PubMed, Elsevier and Google Scholar databases and a total of 32 international clinical studies were included in this non-systematic review. No clinical human study was found in Turkey. Results showed that berry fruits have anti-oxidant, antiinflammatory, anti-diabetes effect and protective effect on the cardiovascular system, effect of improved lipid metabolism, anti-hypertension effect, and reducing effect of colon cancer symptoms and beneficial effects on microbiota by positively affecting human health. More clinical studies are needed to understand the exact mechanisms and long-term effects. [ABSTRACT FROM AUTHOR]

R. acetosella is a perennial herbaceous plant in the Polygonaceae family and is consumed as a vegetable among the people in Turkey. In this study, antimicrobial (Escherichia coli, Bacillus megaterium, Bacillus subtilis, Proteus vulgaris, Pseudomonas aeruginosa, Listeria monocytogenes, Klebsiella pneumoniae, Staphylococcus aureus bacteria and Candida albicans yeast-fungi), antiradical (against to DPPH, ABTS and OH radicals), anticancer (against to human prostate cancer (PC-3), human colon cancer (HCT-116), human ovarian cancer (A2780) and human breast cancer (MCF-7) cell lines) and phytochemical properties (lipid-soluble vitamins, fatty acids, flavonoids, phenolic acids, phytosterols, total phenolic compounds, total flavonoid, and total proanthocyanidins) of ethanol, water and methanol extracts of R. acetosella aerial parts were investigated. According to our results, it was determined that R. acetosella extracts have higher activity than the BHT in scavenging tests of ABTS, DPPH and OH radicals. It has also been understood that this plant has high antimicrobial activity and phytochemical content and effective anticancer activities against to PC-3 human prostate cancer cell lines. Also the presented study is the first study on the in vitro anticancer activity of R. acetosella extracts. [ABSTRACT FROM AUTHOR]

Copyright of Karaelmas Science & Engineering Journal / Karaelmas Fen ve Mühendislik Dergisi is the property of Karaelmas Science & Engineering Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: The aim of this study is to investigate the degree of risk and significance of prognostic and demographic factors affecting the survival of patients diagnosed with colon cancer (CC). Material and Method: This was a retrospective study conducted by reviweing the medical records of 80 patients diagnosed with metastatic CC at the Oncology Clinic at the Mustafa Kemal University between 01/2018 – 01/2021. Cox regression analysis was performed to determine the prognostic and demographic factors affecting the CC and Kaplan-Meier method was used to estimate the overall survival (OS). Difference between the variable levels in terms of survival probabilities was investigated using the log-rank test. Results: The average age of the patients was 56.72 ± 12.42. The median survival time of patients with colon cancer was 48.42 months (95% confidence interval (CI) = 41.97-54.87). Cox regression analysis showed that age, incident type 2 diabetes mellitus (DB), and family history of CC were significantly associated with OS (p lt;0.005). Patients with CC aged 50 years; patients with DB and patients with a family history of CC were approximately 3 times more at risk compared to patients lt;50 years; patients without DB and patients without CC in the family, consecutively. Discussion and Conclusion: The effect of prognostic and demographic factors affecting the survival of patients with CC was evaluated. Our findings showed that age, DB and family history of CC can be used to estimate the survial of patients with CC. We believe that our results will help guide practitioners for the treatment of patients diagnosed with metastatic CC.

This study presents a rare case of unilateral posterior scleritis as an ophthalmic manifestation of a paraneoplastic syndrome. A 61-year-old man presented to our department complaining of gradual worsening of vision in his left eye. Visual acuity was 10/10 and 3/10 in his right and left eye, respectively. He also mentioned that he experienced posterior ocular pain while sleeping at night, but was otherwise asymptomatic. His past ophthalmic and medical history were clear. A thorough clinical, imaging (fundus photography, optical coherence tomography, fluorescein angiography, and B-scan), and laboratory investigation was carried out. A diagnosis of posterior scleritis was made, but no obvious cause or underlying disease was identified even after a thorough systematic assessment. Regular follow-up within the next few months did not reveal any further pathological findings. Finally, 6 months after the initial presentation, the patient was diagnosed with colon cancer. Posterior scleritis can present as an ophthalmic manifestation of a paraneoplastic syndrome in patients with an underlying malignancy, even months before the presentation of systemic symptoms and diagnosis of the underlying disease. In conclusion, in patients (especially older adults) with posterior scleritis, the possibility of a malignant neoplasia must not be ignored or underestimated (paraneoplastic syndrome).

This research was aimed to determine the level of consciousness and consumption status of probiotic foods of the students in the Food Engineering and Chemistry Departments in Sakarya University (Sakarya, Turkey). The study was carried out between April and May-2019 with the participation of 200 students in Chemistry Department and 200 in Food Engineering Department. The research data was collected by using an interview method with a questionnaire. Minitab program was used to evaluate data. Tables with mean, probability value (p value) and percentage (%) values were prepared, and chi-square (χ²) materiality test was used. It was determined that 44% of students in Food Engineering Department and 20.10% of students in Chemistry Department had information about probiotic foods (p<0.05). 32.16% of students in Food Engineering Department and 12.18% of students in Chemistry Department consumed probiotic foods (p<0.05). According to results, students in Food Engineering Department had more information about probiotic foods. Furthermore, students in Food Engineering Department consumed probiotic foods more. In recent years; with the consumption of probiotic foods, digestive system disorders improved significantly, diarrhea decreased, cholesterol level decreased, body cells were renewed, immune system was strengthened and even colon cancer was suppressed. Parallel to this, it was determined that the majority of students consumed probiotic food because of strengthening the immune system. e [ABSTRACT FROM AUTHOR]

Objective In recent years, metallic nanoparticles have attracted the attention in the field of nanomedicine with their antioxidant, antimicrobial and anticancer activities. Especially, metal nanoparticles produced by green synthesis method from medicinal plants with known pharmacological efficacy in cancer treatment have been spotlighted by researchers. Gold nanoparticles; become prominentamong metal nanoparticles with their unique chemical, physical and non-toxic properties. In this study, it was aimed to perform green synthesis of gold nanoparticles using Vitis vinifera (grape seed) aqueous extract and to evaluate cytotoxic, anti-proliferative and apoptotic effects of gold nanoparticles (Vv-AuNP) alone and with Gemcitabine co-administration on HT-29 human colon cancer cell line. Materials and Methods Green synthesis of gold nanoparticles was carried out using the aqueous extract of Vitis vinifera. Vv-AuNPs were characterized by various analytical techniques such as UV-visible spectrophotometer, scanning electron microscope (SEM) and Energy Dispersive X-ray Spectroscopy (EDX). Vv-AuNPs were treated with HT-29 cells at a dose range of 0-80 µg / mL for 72 hours, alone and in combination with Gemcitabine (100 µg / mL or 200 µg / mL fixed dose). Cytotoxic effects were evaluated by MTT assay. In addition, the antiproliferative effects of Vv-AuNPs at increasing doses (0-400 µg / mL) alone and combined with Gemcitabine were determined by the BrdU test. After treatment of gold nanoparticles with HT-29 cells, the level of Caspase-3 activation was analyzed by Western-blot method. Results According to MTT results, when the dose of Vv-AuNPs was increased to 80 µg / mL, it was determined that HT-29 cells did not show the expected level of cytotoxic effect. According to the results of the BrdU cell proliferation test, IC50 values of Vv-AuNPs alone and combined with Gemcitabine were determined as 147.9 and 39.43 µg / mL, respectively. It was observed that the activation of caspase-3 due to Vv-AuNP doses increased compared to control, and its combined use with Gemcitabine reinforced this effect. Conclusion As a result, green synthesized AuNPs exhibited anti-cancer properties on colon cancer cells in vitro. The results of this study show that Vv-AuNPs can be considered as a potential option for colon cancer treatment. [ABSTRACT FROM AUTHOR]

In this review, we present the current innovations in the field of metastatic colon cancer (mCRC). Tumor placement is important in the mCRC treatment decision, and KRAS, NRAS, BRAF mutation analysis is performed in terms of prognosis/predictivity. Immunotherapy is the treatment option in patients with 5-6% and dMMR/MSI. The concept of NeoRAS will become more on the agenda in the near future as high-level evidence becomes available. Molecular evaluations have become extremely important in terms of prognosis and predictions in the treatment of mCRC, as in many tumors today. [ABSTRACT FROM AUTHOR]

Dietary fiber is a plant food component that passes through the human digestive system without undergoing any major changes. It is important in terms of removing harmful compounds accumulated in the intestine, lowering cholesterol by binding bile acids, controlling blood sugar levels and being prebiotic. Dietary fiber prevents constipation by increasing the stool volume in intestines, while reducing the risk of colon cancer by shortening the intestinal transit time. It is generally divided into two sub-groups as water-soluble and insoluble fibers. Water soluble fibers include pectin, gums and mucilage while the latter includes cellulose, hemicellulose and lignin components. Dietary fibers are mainly found in vegetables and fruits, as well as in grains. Factors such as an increase in interest on healthy nutrition, the development of technology, and people's awareness have increased studies on dietary fiber, and it may be expected that it will become one of the indispensable products in many areas of the food industry with an increase in the importance of functional foods in the future. In this study, the properties of dietary fibers and their importance for human health are reviewed. [ABSTRACT FROM AUTHOR]

Objective: Environmental pollution and nutrition with plants and animals exposed to chemical substances such as 1,2-Dimethylhydrazine (DMH) causes many diseases. Colon cancer is one of them. DMH has been found to accumulate in the gastrointestinal tract, impairing the functions of its organs and causing cancer. Zeolite is a volcanic mineral which contains aluminum silicate, which is used in many areas due to its absorption and ion exchange capability, and which has had widespread medical use in recent years. Clinoptilolite, the most well-known species of zeolite, proved to be harmless, did not enter the blood when administered orally to humans and was excreted as a faeces from the gastrointestinal tract. In addition, clinoptilolite administered orally to humans passes through the gastrointestinal tract without dissolving in the blood. Material and Method: The aim of this study was to investigate the effects of clinoptilolite nutrition on colon cancer induced by DMH in rats. The rats were divided into four experimental groups which were control, clinoptilolite, DMH, and clinoptilolite+ DMH using a total of 46 Wistar albino rats. Rats of DMH and clinoptilolite + DMH groups were injected withDMH subcutaneously once a week for 16 weeks at a dose of 20 mg/kg and also clinoptilolite and clinoptilolite+DMH groups were fed a diet with 50% clinoptilolite for eight months. Results: Molecular changes were examined by immunohistochemical staining in colon tissue samples with s-catenin antibody specific for Wnt-s-catenin signaling pathway, which is common in colon cancer. The reaction intensity of s-catenin was determined as DMH> clinoptilolite control> DMH + clinoptilolite, respectively. Conclusion: We may think that clinoptilolite reduces the potential toxic effects of DMH by decreasing cell proliferation [ABSTRACT FROM AUTHOR]

Objective: The most important prognostic factor in early stage Colon Cancer is Lypmh Node (LN) metastasis. In addition, staging is the most important condition for appropriate treatment plan. Therefore, the presence of LN metastasis at the time of diagnosis has a critical role in terms of both correct staging and correct treatment and determining the prognosis. Aim: We aimed to determine the effectiveness of preoperative PET/CT for showing Lymph node metastasis in early stage Colon Cancer Methods: In our study, we retrospectively analyzed the information of 83 early-stage Colon Cancer patients treated in our clinic between 2014 and 2019. Preoperative PET / CT compared with their pathological stages Results: We determined the sensitivity, specificity and accuracy of PET/CT in early stage colon cancer for showing Lymph node metastasis as respectively 66,7%, 64%, 65,1% Discussion: In our study, we did not find satisfactory the sensitivity and specificity of PET/CT for lymph node staging in early stage colon cancer. We found that PET/CT is not more useful than conventional methods in the initial staging of early-stage colon cancer. Therefore, we think that it is more rational to use PET / CT for systemic screening in the initial staging of Colon Cancer or for suspicious lesions. Conclusion: We believe that our study can contribute to the literature in this regard, as it includes a homogeneous patient group with Colon Cancer, but it should be supported by other studies with larger series. [ABSTRACT FROM AUTHOR]

Fourier Transform Infrared (FTIR) spectroscopy is used in studies to determine cancer from blood samples due to its ability to detect chemical changes. The major challenge in studies distinguishing between the FTIR signal of patients and that of healthy people is the lack of clear spectral difference in the FTIR signals. In previous studies, blood samples were dried to overcome this difficulty and peak values or ratios were used in the signal obtained by FTIR measurement. In the proposed method, unlike the literature, plasma samples were measured in liquid form and the resulting FTIR signal then examined as a whole. The FTIR signal was decomposed into sub-bands using wavelet transform. Colon cancer patients and healthy subjects were classified by using the features extracted from the sub-bands. Artificial Neural Networks (ANN), Support Vector Machines (SVM) and k-Nearest Neighbors (k-NN) were used for classification. Colon cancer patients and healthy subjects were classified with an accuracy of 97.14% with SVM. Experimental results indicate that the proposed method may be useful in distinguishing between colon cancer patients and healthy individuals. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Polytechnic is the property of Journal of Polytechnic and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In mammals, the central circadian clock is located in the hypothalamus, coordinating peripheral clocks with neuronal and endocrine signals. It has been shown that circadian clock genes and proteins continue their rhythmic expression in peripheral cells and tissues independent of central clock. The rhythm continues even in the immortalized cell lines, but intercellular coordination is impaired. Cell lines are good mediators for studying cancer-related pathways and circadian rhythm relationships. However, it should first be shown that rhythmic gene expression continues. Some mediators are used to achieve intercellular synchronization. The aim of this study was to investigate the role of strong synchronization initiators, FBS, and dexamethasone, on the expression of the PER2 clock gene in the HT-29 colon cancer cell line. For this purpose; following FBS or dexamethasone administration, HT-29 cells were harvested at 6 different times to isolate RNA and the PER2 and ACTB gene expressions were quantified by real-time PCR assay. The results were evaluated by Linear Cosinor analysis. When the change in mRNA expression of the PER2 gene was compared to the ACTB reference gene, it was shown that rhythmic expression was achieved with both initiators. According to the Cosinor analysis, a significant rhythmic change was observed in both 18 and 24 hour periods. However, in the synchronization with dexamethasone, the confidence interval in the time Period was also significant in the 24 hour period. In this study, it was determined that the initiators may vary in the calculation of circadian Periods which was carried out with two different initiators in HT-29 cell line. [ABSTRACT FROM AUTHOR]

Copyright of Van Tip Dergisi is the property of Yuzuncu Yil University, Faculty of Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: Laparoscopic colon surgery has the clinical benefits of short hospital stay, less pain after surgery and early return of intestinal function. However, hemodynamic changes may occur in the abdominal organs due to prolonged operation time and increased intra-abdominal pressure compared to open surgery. Methods: In this study, we compared the results of laparoscopic colon surgery with the results of open colon surgery in terms of the effects on the abdominal organs and the changes in acute phase reactants. Results: Patients who underwent colon surgery due to benign and malignant diseases in gastrointestinal surgery department between January 2015 and January 2018 were evaluated. Age, sex, operative time, preoperative and postoperative 24th hour blood values (neutrophil, platelet, albumin, alanine transferase, aspartate transferase, total billirubin, urea, creatinine, c-reactive protein) were compared between the two groups. Operative time in group I (open surgery) was significantly lower than group II (laparoscopic surgery) (p < 0.05). In group I, postoperative c-reactive protein (CRP) increase was significantly lower than group II (p<0.05). In group I, postop albumin decrease was significantly higher than group II (p<0.05). Conclusion: Although the operation time was longer in colorectal surgery, there was no significant impairment in renal and liver function in laparoscopic surgery compared to open surgery. Moreover, this study has shown that laparoscopic surgery was less traumatic than open surgery due to minimal change in albumin and CRP values. [ABSTRACT FROM AUTHOR]

Copyright of Turkiye Klinikleri Journal of Internal Medicine / Türkiye Klinikleri İç Hastalıkları Dergisi is the property of Turkiye Klinikleri and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Sosyal Güvence Dergisi is the property of Sosyal Guvenlik Uzmanlari Dernegi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: Cancer is a very common deadly disease in the world. In the cancer formation oxidative stress on the cellular level is thought to be effective for many reasons. The aim of this study was to investigate the changes in oxidative status in cancer types in lung, breast and colon cancers. Methods: Forty-four lung cancer patients, 37 breast cancer patients and 20 colon cancer patients in the medical oncology department were included in the study. All patients were histopathologically diagnosed. Total Antioxidant Levels (TAS), Total Oxidant Levels (TOS) and Catalase (CAT) and Myeloperoxidase (MPO) enzyme activities were measured by photometric method in autoanalyser and compared with 43 healthy volunteers. Results: TOS was significantly higher in all cancer types than control group, but not statistically significant, but significantly higher in breast cancer (p<0.002). Oxidative Stress Index (OSI) were found to be significantly lower in all three types of cancer than in the control group (p<0.001). TAS, CAT and MPO values were significantly higher in all three types of cancer than in the control group (p<0.001). Conclusion: In the study, it was concluded that oxidative stress increased in cancer, but increased oxidative stress may decrease OSI level due to induction of antioxidant defense system. [ABSTRACT FROM AUTHOR]

Many of the colorectal cancers originated from polips. We wanted to discuss a case accompanied with literature who has a complicated giant rectal polip which presented with an unusual clinic and confusion in diagnosis. 36 years old male patient. He has cramp like stomache and changing in volonic habits for about a year. During colonoscopy a malign mass in caecum and a giant villous adenoma in rectum is identified. The mass in caecum is excised via laparoscopic surgery. The mass in rectum received polipectomy via local excision. Altough a cancer has seen in the polip the surgery is considered opprepriote because of the surgical frontier and acceptable histologic properties. Colorectal polips are lesions which has the risk to turn into cancers thus, detoiled histopatologic examination and gently decission of surgery is needed. [ABSTRACT FROM AUTHOR]

DUAs with all cancer types, the initiation, development, and metastatic stages of prostate and colorectal cancers, are very important to understand the relationship between tumor cells and normal cells in the stroma, the changes in gene and signaling pathways, as well as their behavior towards the immune response. Therefore, for experimental research, the formation of tumor cell lines, the formation of tissues in the laboratory environment, and the formation of tumor models via injection or implantation of animal or human tumor cells or tissues in experimental animals, which are the silent heroes of science, and their molecular imaging are very important. Only in this way can there be an effective treatment of these diseases, or they can be prevented before they occur. [ABSTRACT FROM AUTHOR]

76 years old female patient applied our clinic with bilateral painless decreased vision for 5-6 months. Best corrected visual acuities were 20/640 in the right, 20/500 in the left eye. Slit lamp examination was unremarkable except bilateral pseudophakia. Intraocular pressures were within normal limits. Bilateral severe cystoid macular edema was identified in indirect retina examination and optical coherence tomography (OCT). Patient was initiated oral capecitabine therapy 10 months ago and the therapy was ceased 4 months ago. There wasn't any other topical or systemic medication history except capecitabine. After the treatment with oral acetazolamide and topical steroid and non-steroidal drops, best corrected visual acuities increased to 20/200 in right eye and 20/63 in the left eye. In OCT examination cystoid macular edema was decreased in the right eye and disappeared in the left eye. This is the first case reporting bilateral cystoid macula edema related to capecitabine therapy in the literature. [ABSTRACT FROM AUTHOR]

Aim: The aim of this study is to synthesize zinc oxide nanoparticles (ZnONPs) by green chemistry method and investigate anti-bacterial and anticancer effects of these nanoparticles. Material and Methods: ZnONPs were synthesized by the green chemistry method using zinc ions and aqueous artichoke leaf (Cynara scolymus) extract. Ultraviolet-visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), zetasizer and energy dispersive X-ray spectroscopy (EDX) were used to confirm the formation and characterization of ZnONPs. Antibacterial activities of ZnONPs on four different bacteria species (E. coli, S. aureus, P. aeruginosa and E. faecalis) were measured by minimal inhibitory concentration (MIC) and agar well diffusion method. Cytotoxic effects of ZnONPs on HT-29 human colon cancer cells were determined as concentration and time dependent. Results: In the UV-Vis spectrum, absorbance increase was observed in 320-335 nm range which is specific to ZnO. In the FTIR spectrum, stretching vibrations of ZnO were determined in 426 cm-1 and 540 cm-1. The particle size was 276-309 nm in SEM analysis. In the zeta-sizer measurements of ZnONPs, the particle size was 137.8 nm and the particle charge was -6.34 meV. In the antibacterial activity measurements, it was determined that synthesized nanoparticles induce inhibition of bacterial activity in E. coli and S. aureus. ZnONPs showed cytotoxic effects on HT-29 cells at concentrations higher than 10 µg/mL. Conclusion: This study showed that ZnONPs can be prepared at low cost and have potential to be used as a carrier system for new drug formulations in clinical therapies. [ABSTRACT FROM AUTHOR]

Hepatitis B virus (HBV) and hepatitis C virus (HCV) are associated with significant morbidity and mortality among patients with cancer who received cytotoxic chemotherapy. To our knowledge, there are not satisfying available data in regard to hepatitis B surface antigen (HBsAg) positivity ratios in solid cancer patients. Prevalence and reactivation rates of chronic HBV and HCV infections in gastrointestinal cancer patients were retrospectively investigated. Statistical significant relationship was detected for HBsAg positivity in gastric and esophageal cancers (5.67%) and rectum cancer (5.6%). Statistical significant relationship was not detected for HCV. Screenings for viral hepatitis is required in order to prevent treatment discontinuations. [ABSTRACT FROM AUTHOR]

Colorectal cancer during reproductive ages is a rare diagnosis and it is usually of advanced stage when the diagnosis is made during pregnancy. In our case the diagnosis was made with detection of liver metastases . After the diagnosis an elective caesarean section was made both for treatment and intra-operative evaluation of abdominal cavity for future treatment procedures. Intra-operative evaluation revealed miliary disseminated liver metastases and deemed unresectable. After the operation chemotherapy was initiated however initial response to therapy was unsatisfactory and patient progressed to terminal stage shortly after. Due to overlapping symptomps of early colorectal cancer and pregnancy diagnosis is usually made through symptoms of advanced disease such as metastases. When a colorectal cancer diagnosis is made during pregnancy, disease is often of advanced nature and prognosis is poor. [Cukurova Med J 2015; 40(4.000): 807-810]

Reprodüktif dönemde kolorektal kanser çok nadir bir durumdur ve kolon kanseri ile komplike gebeliklerde tanı genellikle ileri evrede olmaktadır. Bizim vakamız 36 yaşında ve 3.trimesterde tanısı konabilen ileri evre bir kolon kanseri vakasıdır. Hastalığın ilk dönemi asemptomatik olarak seyretmiştir. Karaciğer metastazı olduktan sonra bulgu vermiştir. Tanı sonrasında tedavi için doğum gerçekleştirilmiştir ve hasta postoperatif dönemde kemoterapi almaya başlamıştır. Kolon kanseri reprodüktif dönemde kolon kanserinin az görülmesi ve gebeliğin kolon kanserinin semptomlarını taklit etmesi nedeniyle erken tanıda şansı çok düşük olan bir hastalıktır, genellikle ileri evrede tanı almaktadır. Bundan dolayı kötü prognozludur. Bizim hastamızda ileri evrede yakalanabilen bir hastaydı. Bundan dolayı tanı sonrasında kısa sürede gebelik sonlandırılıp tedavisi başlanmış olsa bile prognozu kötü olmuştur ve hasta kısa süre içinde terminal döneme geçmiştir. Sonuçta kolon kanserinin her ne kadar erken tanı şansı olsa da genç yaşta ve gebelikle birlikte olan vakalarda bu şans daha da azalmaktadır

Copyright of Gümüshane Üniversitesi Saglik Bilimleri Dergisi is the property of Gumushane University, Faculty of Health Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abdominal pain is one of the basic clinical presentations of the hereditary angioedema and danazol is a common medicine which has been used for long years in patients with hereditary angioedema. We present two hereditary angioedema patients with abdominal pain albeit under danazol treatment, whose final diagnoses was colon carcinoma. There are two consequences in this article which shall be insisted on: First; in patients with hereditary angioedema, the differential diagnosis of and ldquo;abdominal pain and rdquo; is always important even though hereditary angioedema diagnosis exists. And the second; It can be hardy speculated that long term danazol treatment may cause different malignancies. [Cukurova Med J 2016; 41(3.000): 567-569]

Aim:The aim of this study is sharing the morbidity and mortality rates and risc factors efecting the morbidity and mortality rates of patients underwent emergency operation in our clinic.Materials and Methods:Between January 2008 - July 2012 eighteen patients, 10 men and 8 women, who operated because of obstructive colorectal cancer, were reviewed. Patients were examined fora ge, sex, complication, operation type, morbidity and mortality. The effects of age, sex and tumor location on morbidity and mortality were examined.Results:Mean age of patients was 66 ± 8,6. Ten patients were elder than 70 (% 56,6). Complet obstruction was seen in 16 patients (% 88,8). In one patient perforation was seen wtih obstrucion (% 5,6). Hartmann’s procedure was performed for 12 patients (% 66,7), loop colostomy was performed for two patients (% 11,2), right hemicolectomy was performed for two patients (% 11,2), total colectomy with ileorectal anastomosis was performed for 1 patient (% 5,6), right hemicolectomy with end ileostomy was performed for 1 patient (% 5,6). Morbidity was seen in 5 patients (% 27,8). Mortality was seen in two patients (% 11,2). Both patients with mortality were elder than 70 (p=0.21). There weren’t any significant difference for sex and tumor’s location.Conclusion:Morbidity and mortality rates increases in patients whom underwent emergency surgery for obstructive colorectal cancer. Being elder patient is one of the reasons. And also accompanied complications like perforation searously increase mortality and morbidity.

Aim: Nurses have an indispensable role in knowing the cancer screening programs, education of society related to identifying risks groups and importance of early detection methods, determining the signs and symptoms of cancer. In this study, it is aimed to determine the knowledge of nurses working in a tertiary health care institution about cancer screening programs and Cancer Early Diagnosis, Screening and Training Centers (KETEM), and status of screening tests. Material and Methods: The research conducted between February and March 2016, is a descriptive study and carried out with 325 nurses working at Adnan Menderes University Application and Research Hospital. The 18-item questionnaire form was applied to participants by face to face interview method. Descriptive statistics are given as the frequency and percentage for categorical data, and the median (minimum-maximum) values for numerical data by examining the normal distribution. Results: The median age of participants is 25, and 85.5% of them are females. 30.1% of the participants stated that they had a family history of cancer, and only 9.1% of them were recommended for screening. One out of every five participants did not hear KETEM. 32% of the participants stated that they learned about cancer screening from television-radio after the graduation. Only 46.4% of participants answered the goal of cancer screenings exactly as 'catching cancer in early stage'. 20.7% of the participants over age 30 have had pap-smear and 45.4% of the participants over age 40 have had mammography. Conclusion: This study has shown that participants' knowledge of KETEM and cancer screening programs aren't sufficient. The increase in nurses' own awareness will also increase the awareness of the community. Nurses should be provided with in-service training to update knowledge throughout their professional life. Thus, accurate and complete information will be provided for both themselves and the consulting services they will provide. [ABSTRACT FROM AUTHOR]

Skleroderma cilt altında ve iç organlarda artmış kollajen birikimi ile karakterize bir bağ dokusu hastalığıdır. Etiyolojisi tam aydınlatılamamakla birlikte bazı kimyasal ajanların ve çevresel faktörlerin etyopatogenezde rol oynadığı kabul edilmektedir. Progresif sistemik skleroderma olan olgularda gastrointestinal kanal karsinomlarının arttığına dair sınırlı veri bulunmaktadır. Kolon karsinomu tedavisinde kullanılan 5-florourasil ile skleroderma ilişkisine dair literatürde sadece iki olgu bildirilmiştir. 5-florourasil ve folinik asit tedavisi verilen timik karsinomlu bir olguda ise skleroderma benzeri klinik tablo ve otoantikor pozitifliği bildirilmiştir. Bu yazıda, 51 yaşında kolon karsinomu tanısıyla 3 kür 5-florourasil ve folinik asit tedavisi alan ve daha sonra scleroderma geliştiren bir olgu sunulmuştur.

Sistemic sclerosis is a connective tissue disease which is characterized by the increase amount of collagene in subcutaneous tissue and internal organs. Several chemical agents and environmental factors are accused fort he etiopathogenesis however the etiology is not clear. There is limited data abput the increased frequency of colon carcinoma in the cases with sistemic sclerosis. There are 2 cases in the literature about the association between scleroderma and 5-fluorouracil which is used for the treatment of colon carcinoma. Similar clinical course and autoantibody positivity was also reported in a patient with tymic carcinoma who had been treated with 5-FU and folinic acid. In this article, a 51-year-old woman who developed scleroderma after 5-FU and folinic acid therapy which had been started for colon carcinoma has been reported. [Cukurova Med J 2014; 39(2.000): 362-364]