HT-29 Kolon Kanser Hücre Hattında Senkronizasyon Başlatıcıların Sirkadiyen Ritimdeki Rolü.

In mammals, the central circadian clock is located in the hypothalamus, coordinating peripheral clocks with neuronal and endocrine signals. It has been shown that circadian clock genes and proteins continue their rhythmic expression in peripheral cells and tissues independent of central clock. The rhythm continues even in the immortalized cell lines, but intercellular coordination is impaired. Cell lines are good mediators for studying cancer-related pathways and circadian rhythm relationships. However, it should first be shown that rhythmic gene expression continues. Some mediators are used to achieve intercellular synchronization. The aim of this study was to investigate the role of strong synchronization initiators, FBS, and dexamethasone, on the expression of the PER2 clock gene in the HT-29 colon cancer cell line. For this purpose; following FBS or dexamethasone administration, HT-29 cells were harvested at 6 different times to isolate RNA and the PER2 and ACTB gene expressions were quantified by real-time PCR assay. The results were evaluated by Linear Cosinor analysis. When the change in mRNA expression of the PER2 gene was compared to the ACTB reference gene, it was shown that rhythmic expression was achieved with both initiators. According to the Cosinor analysis, a significant rhythmic change was observed in both 18 and 24 hour periods. However, in the synchronization with dexamethasone, the confidence interval in the time Period was also significant in the 24 hour period. In this study, it was determined that the initiators may vary in the calculation of circadian Periods which was carried out with two different initiators in HT-29 cell line. [ABSTRACT FROM AUTHOR]