1. The murine pan T cell marker CD96 is an adhesion receptor for CD155 and nectin-1
- Author
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Seth, S., Maier, M.K., Qiu, Q., Ravens, I., Kremmer, E., Förster, R., and Bernhardt, G.
- Subjects
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IMMUNOGLOBULINS , *T cells , *MONOCLONAL antibodies , *CELL communication - Abstract
Abstract: The CD155 ligand CD96 is an immunoglobulin-like protein tentatively allocated to the repertoire of human NK receptors. We report here that the CD96/CD155-interaction is preserved between man and mouse although both receptors are only moderately conserved in amino acid sequence. Moreover, murine CD96 (mCD96) binds to nectin-1, a receptor related to CD155. Applying newly generated monoclonal antibodies specifically recognizing mCD96, an expression profile is revealed resembling closely that of human CD96 (hCD96) on cells of hematopoietic origin. A panel of anti-mCD96 but also recently established anti-mCD155 antibodies effectively prevents formation of CD96/CD155-complexes. This was exploited to demonstrate that the only available receptor for mCD96 present on thymocytes is mCD155. Moreover, T cell adhesion to insect cells expressing mCD155 is blocked by these antibodies depending on the T cell subtype. These results suggest a function of the CD96/CD155-adhesion system in T cell biology. [Copyright &y& Elsevier]
- Published
- 2007
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