Back to Search
Start Over
Low serum neutralizing anti-SARS-CoV-2 S antibody levels in mildly affected COVID-19 convalescent patients revealed by two different detection methods.
- Source :
-
Cellular & molecular immunology [Cell Mol Immunol] 2021 Apr; Vol. 18 (4), pp. 936-944. Date of Electronic Publication: 2020 Nov 02. - Publication Year :
- 2021
-
Abstract
- Neutralizing antibodies targeting the receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) block severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) entry into cells via surface-expressed angiotensin-converting enzyme 2 (ACE2). We used a surrogate virus neutralization test (sVNT) and SARS-CoV-2 S protein-pseudotyped vesicular stomatitis virus (VSV) vector-based neutralization assay (pVNT) to assess the degree to which serum antibodies from coronavirus disease 2019 (COVID-19) convalescent patients interfere with the binding of SARS-CoV-2 S to ACE2. Both tests revealed neutralizing anti-SARS-CoV-2 S antibodies in the sera of ~90% of mildly and 100% of severely affected COVID-19 convalescent patients. Importantly, sVNT and pVNT results correlated strongly with each other and to the levels of anti-SARS-CoV-2 S1 IgG and IgA antibodies. Moreover, levels of neutralizing antibodies correlated with the duration and severity of clinical symptoms but not with patient age. Compared to pVNT, sVNT is less sophisticated and does not require any biosafety labs. Since this assay is also much faster and cheaper, sVNT will not only be important for evaluating the prevalence of neutralizing antibodies in a population but also for identifying promising plasma donors for successful passive antibody therapy.
- Subjects :
- Adult
Aged
Angiotensin-Converting Enzyme 2 metabolism
COVID-19 blood
Cell Line
Convalescence
Female
Humans
Immunoglobulin A blood
Immunoglobulin G blood
Male
Middle Aged
Neutralization Tests methods
Antibodies, Neutralizing blood
Antibodies, Viral blood
COVID-19 immunology
Spike Glycoprotein, Coronavirus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2042-0226
- Volume :
- 18
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cellular & molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 33139905
- Full Text :
- https://doi.org/10.1038/s41423-020-00573-9