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CD155 is involved in negative selection and is required to retain terminally maturing CD8 T cells in thymus.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2010 Feb 15; Vol. 184 (4), pp. 1681-9. Date of Electronic Publication: 2010 Jan 04. - Publication Year :
- 2010
-
Abstract
- During their final maturation in the medulla, semimature single-positive (SP) thymocytes downregulate activation markers and subsequently exit into the periphery. Although semimature CD4(+) SP cells are sensitive to negative selection, the timing of when negative selection occurs in the CD8 lineage remains elusive. We show that the abundance of terminally matured CD8(+) SP cells in adult thymus is modulated by the genetic background. Moreover, in BALB/c mice, the frequency of terminally matured CD8(+) SP cells, but not that of CD4(+) SP cells present in thymus, varies depending on age. In mice lacking expression of the adhesion receptor CD155, a selective deficiency of mature CD8(+) SP thymocytes was observed, emerging first in adolescent animals at the age when these cells start to accumulate in wild-type thymus. Evidence is provided that the mature cells emigrate prematurely when CD155 is absent, cutting short their retention time in the medulla. Moreover, in nonmanipulated wild-type mice, semimature CD8(+) SP thymocytes are subjected to negative selection, as reflected by the diverging TCR repertoires present on semimature and mature CD8(+) T cells. In CD155-deficient animals, a shift was found in the TCR repertoire displayed by the pool of CD8(+) SP cells, demonstrating that CD155 is involved in negative selection.
- Subjects :
- Aging genetics
Aging immunology
Animals
Apoptosis genetics
Apoptosis immunology
CD8-Positive T-Lymphocytes metabolism
Cell Differentiation genetics
Cell Movement genetics
Cellular Senescence genetics
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred DBA
Mice, Knockout
Receptors, Virus deficiency
Receptors, Virus genetics
Thymus Gland metabolism
CD8-Positive T-Lymphocytes cytology
CD8-Positive T-Lymphocytes immunology
Cell Differentiation immunology
Cell Movement immunology
Cellular Senescence immunology
Receptors, Virus physiology
Thymus Gland cytology
Thymus Gland immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 184
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 20048123
- Full Text :
- https://doi.org/10.4049/jimmunol.0900062