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[Objective] The study aimed to investigate the effect of macular pollutants on the release of pyrolytic products from cigarette paper, and provide a method basis for the classification of macular pollutants. [Method] The pyrolytic particulate matter of different cigarette papers was captured by a self-built temperature-controlled pyrolytic device. The differences in pyrolytic components of different cigarette paper samples were analyzed by using GC-MS. And the classification of macular pollutants was achieved using principal component and cluster analysis based on the differences in pyrolytic products. [Result] The different pollutants changed the microscopic surface morphology of cigarette paper fibers. And the pyrolytic products chromatograms of all the contaminated cigarette papers exhibited a similarity of less than 0.417 when compared with that of blank cigarette papers. A total of 78 kinds of pyrolytic products were detected in the blank cigarette papers, including ketones, phenols, benzene derivatives, aldehydes, furans, alcohols, heterocycles and other chemicals, etc. Moreover, the pyrolytic products released from contaminated cigarette paper not only had common components with blank cigarette paper, but also had many unique pyrolysis components, and there were significant differences in the number and content distribution of various pyrolysis components. Specifically, after the contamination of Premalube red and Nordic grease, the phenolic compounds in the pyrolytic products significantly reduced from 36.07% to 5.58% and 8.32%, respectively. Meanwhile the release of hydrocarbons remarkably increased, but the composition and distribution of hydrocarbon substances exhibited significant differences. Flavor and sugar contaminants reduced the content of aldehydes, ketones, phenols, benzene rings and heterocyclic pyrolytic components in the pyrolytic products, but their own unique propylene glycol/glycerol solvents or esters could release to the particulate matter during the heating process through the evaporation. Principal component analysis based on pyrolytic products showed that cigarette papers contaminated with different pollutants could be effectively distinguished from the blank sample. Hierarchical clustering analysis also showed that samples contaminated with grease could be classified into one, and cigarette papers contaminated with flavor and sugar could be grouped into another one. [Conclusion] The results in the study would be beneficial for the classification and identification of macular pollutants source based on the differences in pyrolytic products that were captured by self-built temperature-controlled pyrolytic device and analyzed by multiple statistical methods. [ABSTRACT FROM AUTHOR]

[ Objective ] The study aimed to research on the effect of β-cyclodextrin on aroma retention of paper-making reconstituted tobacco, this experiment was carried out. [ Methods ] The reconstituted tobacco was prepared according to the conventional paper-making process, and β-cyclodextrin was added into the tobacco raw material during the extraction process. The aroma components of the extraction concentrate were extracted by two-step simultaneous distillation extraction, and the free and combined aroma components were quantitatively detected by GC-MS internal standard. The difference of free and combined aroma before and after adding β-cyclodextrin was compared and analyzed. [ Results ] After adding β-cyclodextrin, the total amount of free aroma components decreased, while the combined aroma components in creased. After 8 hours of extraction, the total amount of aroma components extracted from the concentrate decreased by 12. 75% . It should be that after 8 hours of simultaneous distillation and extraction, some of the volatile aroma components were in the complex state with β-cyclodextrin, but not released by extraction. [ Conclusion ] β-cyclodextrin can slow release and keep the aroma of the extract and concentrate of paper-making reconstituted tobacco. [ABSTRACT FROM AUTHOR]

[Objective] The study aimed to elucidate the effects of two kinds of organic potassium salt cigarette auxiliaries on the pyrolysis performance of cigarette paper. [Method] By adding two kinds of organic acid potassium salt with different concentrations to cigarette paper, the effects on the pyrolysis performance of cigarette paper were studied by means of simultaneous thermal analysis, mainstream smoke detection and sensory quality evaluation. [Result] With the increase of the concentration of potassium carbonate and potassium citrate, the initial temperature of thermogravimetric loss gradually decreased, and the increase of weight loss rate and weight loss in the initial heating period corresponded basically with the addition concentration. With the addition of potassium citrate in the cigarette paper, the number of smoking pons decreased gradually, and the content of nicotine and tar also decreased gradually. When the addition amount was 3 %, the release of CO was the lowest, and then increased with the increase of the addition amount. With the addition of potassium carbonate in cigarette paper increasing, the number of smoking mouthfuls firstly decreased and then increased slowly, and the content of nicotine and tar also showed a decreasing trend. When the addition amount of potassium carbonate in cigarette paper was 3.5 %, the content of nicotine in cigarette paper and the number of smoking mouthfuls were the least. When the concentration was 2.5 %, the CO content was the lowest. [Conclusion] The use of cigarette paper additives catalyzed the thermal cracking process of cigarette paper, which resulted in the decrease of tar and CO content in mainstream smoke and affected the sensory quality of cigarettes. [ABSTRACT FROM AUTHOR]

[Objective]The present paper aimed to search for some technical methods which could reduce the tar content of reconstituted tobacco effectively. [Method]The effect of some factors such as ratio of stems to leaves, beating degree, basis weight, coating rate, proportion of plus fiber and calcium carbonate on the tar content of paper-making reconstituted tobacco was studied and those factors that could reduce the tar content effectively had also been verified. [Result] (i)Beating degree, basis weight, proportion of plus fiber, potassium salt and calcium carbonate had significant influence on the tar content of paper-making reconstituted tobacco;(ii)The ratio of stems to leaves of raw materials also had a certain influence on the tar content of paper-making reconstituted tobacco, and the tar content was the lowest when the ratio of stems to leaves was 2-1;(iii)There was a positive correlation between the coating rate and the tar content of paper-making reconstituted tobacco, the increase of coating rate would lead to the increase of tar content.(IV) The tar content of paper-making reconstituted tobacco prepared under a set of factors, including fiber proportion, beating degree, basis weight had fallen by more than 50 % ,and blending it in cigarette products had also reduced the tar content of cigarettes obviously. [Conclusion] The tar content of reconstituted tobacco could be reduced by adjusting the process parameters. While the PRT sample integrated with some tar-reduced factors was blended into cigarettes, it could greatly reduce the tar content of cigarettes. Therefore, some technical methods could be taken properly to reduce the tar content of PRT, and achieve ‘ reducing the harmful and tar content’ after blending it into cigarettes. [ABSTRACT FROM AUTHOR]

[Objective] Feasibility study of applying writing paper as sealing materials in oyster mushroom’s cultivation was conducted in or¬der to provide theoretical basis for increasing the safety of oyster mushroomproducts. ' Method】 The experiments and production demonstration were carried out by setting 2 treatments, ie. treatment Awith writing paper as sealing materials and treatment Bwith newspapers! CK) ,and the growth of mycelium, number and fresh weight of fruiting bodies, cost and economic returns as well as heavy metal contents in fruiting bodies were investigated and evaluated. ' Result】 The growth rate of mycelium was 0. 97 cm/d of treatment A, which was significantly higher than that of treatment B (% < 0 . 05 , the same as below), and the average number and fresh weight of fruiting bodies per bag (total of the first flush and second flush, hereafter) were 43.47 ,257. 59 g,5. 57 more and 11 . 7 g higher than that of treatment B, respectively. The results showed that the heavy metal content of Pb, Cd, Hg and As in fruiting bodies of both treatment A and Bwas lower than the limits (1 . 000, 0. 200 ,0. 100,0. 500 mg/kg, respectively) set by both national food safety ytandard and national aaricultural standard for green food mush¬ room, and the content of Pb, Cd, Hg, As of treatment A was only 0. 001, 0. 085 , 0.002 and 0. 037 mg/kg, which was far more lower tlian the safety limits mentioned above, respectively. Besides, Cr was not found in the fruiting bodies of treatment A. The results indicated that the oyster mushroom of treatment A was much safer than that of treatment B. Compared to treatment B， the cost per bag of sealing materials and their processing increased by 0.002 yuan， while the output valiae increased by 0. 140 yuan, thus the eco¬nomic retiams per bag of treatment A was 0. 138 yuan higher than that of treatment B. The results of production demonstra¬tion showed that，when compared witli treatment B，treatment A could shorten the period of spawn running by 3 days and shorten the average harvesting time of the first and second flush by 3 and 4 days, respectively, besides, treatment Acould increase thefresh weight per bag by 24. 00 gin total. ' Conclusion】 Based on the in¬crease of mycelium ’ s growth rate, number and fresh weight of fruiting bodies, economic returns, as well as decrease of heavy metal contents, it is recommended that writing paper could be applied instead of newspapers as sealing materials in oyster mushroom5 s production [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to get high efficient cellase-producing bacteria and determine the taxonomy. [Method] Sodium carboxymethylcellulose was selected as the sole carbon source to isolate cellulose-producing microorganism. A total of 51 bacteria and actinomyces were isolated from natural fermentation material of tobacco straw.Nine isolates possessed high cellulase activities in congo dye experiment. The phylogenetic relationships of cellulase producing bacteria and actinomyces were determined through the analyses of phylogenetic trees constructed using 16 S rRNA gene sequences. [Result] Luteibacter sp.L43 possessed the highest cellulase activities in enzyme activity tests. The effects of the single factor including pH, carbon and nitrogen concentration on filter paper enzyme, endo-β-1, 4-glucanases and exo-β-1, 4-glucanases for L43 were determined.Under the condition of pH6, sodium carboxymethylcellulose concentration 12.5 g/L and NaNO3 concentration 5 g/L, the highest cellulase activities of endo-β-1, 4-glucanases and exo-β-1, 4-glucanases of L43 were 16.9 and39.62 U/mL, respectively. [Conclusion] This study provided a good cellulose producing bacteria for tobacco straw fermentation and laid a foundation for the development and utilization L43. [ABSTRACT FROM AUTHOR]

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