1. Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera
- Author
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Yu Zhou, Weihua Gao, Mingyuan Liu, Xiaolei Liu, Hualin Yang, Yan-Song Li, Liu Yan, Ning Xu, and Bin Tang
- Subjects
Life Cycles ,Nematoda ,Swine ,Physiology ,Trichinella ,RC955-962 ,Pathology and Laboratory Medicine ,Biochemistry ,Serology ,Mice ,Larvae ,Medical Conditions ,Arctic medicine. Tropical medicine ,Immune Physiology ,Medicine and Health Sciences ,Parasite hosting ,Enzyme-Linked Immunoassays ,Nematode Infections ,Swine Diseases ,Mammals ,Clonorchis sinensis ,Immune System Proteins ,biology ,Eukaryota ,Trichinellosis ,Helminth Proteins ,Recombinant Proteins ,Infectious Diseases ,Vertebrates ,Public aspects of medicine ,RA1-1270 ,Antibody ,Research Article ,medicine.drug_class ,Trichinella spiralis ,Immunology ,Antibodies, Helminth ,Enzyme-Linked Immunosorbent Assay ,Monoclonal antibody ,Research and Analysis Methods ,Virus ,Antibodies ,medicine ,Parasitic Diseases ,Animals ,Humans ,Immunoassays ,Public Health, Environmental and Occupational Health ,Organisms ,Biology and Life Sciences ,Proteins ,biology.organism_classification ,Virology ,Cystatins ,Invertebrates ,Amniotes ,biology.protein ,Immunologic Techniques ,Zoology ,Developmental Biology - Abstract
Objectives Trichinella spiralis is a zoonotic parasite with a complex parasitic life cycle and exposed to animals or humans by infectious meat. To control transmissions of T. spiralis through the food chain to humans, sensitive and selective multihost sera-diagnosis is urgent needed for monitoring T. spiralis exposure. Methods A competition enzyme-linked immunosorbent assay (cELISA) for T. spiralis infection diagnosis in multihost sera was developed based on recombinant cystatin-like protein (rCLP-cELISA) as well as monoclonal antibodies. The sensitivity and accuracy of the rCLP-cELISA were quantified using swine (n = 1316), mice (n = 189) and human (n = 157) serum samples. T. spiralis-antibody targeting test ability of the rCLP-cELISA in swine (n = 22) and human (n = 36), instead of other parasites or viruses antibodies, was evaluated. Results The rCLP-cELISA showed high agreement with commercial ELISA kits in field swine sera assessed by Cohen’s kappa value (κ = 0.7963). And it showed 100% specificity in human trichinellosis detection with sensitivity of 96.49%, no cross-reaction with other parasite or virus infections, and high positive detection rate of 87.5% in low-dose infected swine. Besides, the rCLP-cELISA exhibited potential in the detection of T. spiralis, T. nelsoni and Trichinella T8 infections. Conclusions The rCLP-cELISA can be used for T. spiralis-associated antibody test in multihost sera., Author summary Infections with T. spiralis that lives in host muscles for long periods of time are commonly difficult to diagnosis without causing a strong immune response or symptoms. The habit of eating raw/undercooked pork meat accidentally introduces humans into the exposure of T. spiralis that circulates between herds and rodents. There is an urgent need for serological antibody test of T. spiralis to monitor the infection of humans as well as hosts in the food chain, which is restricted by the mixture type of current used antigens and species-specific secondary antibodies for different hosts. We developed a novel recombinant cystatin-like protein-based competition enzyme-linked immunosorbent assay (rCLP-cELISA) employing monoclonal antibody. The proposed method showed considerable sensitivity and specificity in filed swine sera and human serum samples with good versatility in mice. Taking advantage of its controllable quality stability, the rCLP-cELISA had potential applications for screening of T. spiralis infections for multihost sera in one test. With the development of monoclonal antibody modification strategies and the discovery of antigenic proteins from parasitic pathogens, the proposed competition ELISA also provides useful reference for the improvement of serological assay for monitoring the exposure of zoonotic parasites.
- Published
- 2021