101. Saccharification of pretreated empty bunch by concentrated xylanase and assisted by cellulase.
- Author
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Wijaya, Hans, Kahar, Prihardi, Sasaki, Kengo, Rahmani, Nanik, Hermiati, Euis, Yopi, Ogino, Chiaki, Prasetya, Bambang, Simarmata, Rumella, Christita, Margaretta, and Kondo, Akihiko
- Subjects
CELLULASE ,XYLANASES ,MEMBRANE separation ,ETHANOL ,LIGNOCELLULOSE ,FILTER paper ,SACCHAROMYCES cerevisiae ,NANOFILTRATION - Abstract
The effect of the membrane separation process to concentrate the xylanase was tested to restrict cellulase dosage on the lignocellulosic waste. Xylanase produced from Streptomyces lividans expressing an endo-xylanase gene from Kitasatospora sp. was concentrated by nanofiltration membrane NTR-7410. This resulted in a seven-fold increase in xylanase activity without changing its specific activity. The saccharification of dilute acid-pretreated empty fruit bunch (EFB) was performed by applying membrane-concentrated xylanase compared with its un-concentrated to commercial cellulase loaded at one filter paper unit/g-biomass. Both glucose and xylose yields obtained by using concentrated xylanase (19.49% and 41.51%, respectively) were higher compared to un-concentrated xylanase (11.71% and 28.71%, respectively). Xylose-assimilating Saccharomyces cerevisiae yeast at initial of 0.5 g/L was then inoculated into the sugar solutions. The yeast had increased ethanol titer and productivity from sugars obtained by using membrane-concentrated xylanase (7.13±0.60 g/L and 0.59±0.06 g/L/h, respectively), compared by using un-concentrated xylanase (4.56±0.43 g/L and 0.38±0.02 g/L/h, respectively). [ABSTRACT FROM AUTHOR]
- Published
- 2023
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