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Additional file 4: Figure S2. Dwell time distribution of AtRGS1-YFP and mCherry-AtREM1.3 on the PM analyzed by traditional kymography methods under different conditions. The 6-day-old transgenic seedlings expressing AtRGS1-YFP (A, B) and mCherry-AtREM1.3 (C, D) were treated with ½ MS liquid medium (CK) and 100 μM MeJA (JA) for 8 h. Bar = 2 s.

Additional file 3: Figure S1. SCI of AtRGS1-YFP or mCherry-AtREM1.3 analyzed by ImageJ software under different conditions. The 6-day-old transgenic seedlings expressing AtRGS1-YFP (A) and mCherry-AtREM1.3 (B) were treated with ½ MS liquid medium (CK) and 100 μM MeJA (JA) for 8 h by TIRFM observation. ***P < 0.001. ns > 0.05. Student’s t-test. Error bars represent the SD. n = 32 to 67 per treatment.

Additional file 6: Figure S4. Colocalization between AtRGS1-YFP and mCherry-AtREM1.3 analyzed by traditional kymography under different conditions. A–C Typical 2D viewer of AtRGS1-YFP (A), mCherry-AtREM1.3 (B), and merge (C) images at steady state. D–F Typical 2D viewer of AtRGS1-YFP (D), mCherry-AtREM1.3 (E), and merge (F) images with JA treatment. Bar = 3 s. The 6-day-old transgenic seedlings co-expressing AtRGS1-YFP and mCherry-AtREM1.3 were treated with ½ MS liquid medium (CK) and 100 μM MeJA (JA) for 8 h.

Additional file 5: Figure S3. Colocalization between AtRGS1-YFP and mCherry-AtREM1.3 analyzed by VA-TIRFM under different conditions. A–C Typical single-molecular viewer of AtRGS1-YFP (A), mCherry-AtREM1.3 (B), and merge (C) images at steady state. D–F Typical single-molecular viewer of AtRGS1-YFP (D), mCherry-AtREM1.3 (E), and merge (F) images upon JA treatment. Bar = 5 μm. The 6-day-old transgenic seedlings co-expressing AtRGS1-YFP and mCherry-AtREM1.3 were treated with ½ MS liquid medium (CK) and 100 μM MeJA (JA) for 8 h.