Additional file 6 of VA-TIRFM-based SM kymograph analysis for dwell time and colocalization of plasma membrane protein in plant cells

Additional file 6: Figure S4. Colocalization between AtRGS1-YFP and mCherry-AtREM1.3 analyzed by traditional kymography under different conditions. A–C Typical 2D viewer of AtRGS1-YFP (A), mCherry-AtREM1.3 (B), and merge (C) images at steady state. D–F Typical 2D viewer of AtRGS1-YFP (D), mCherry-AtREM1.3 (E), and merge (F) images with JA treatment. Bar = 3 s. The 6-day-old transgenic seedlings co-expressing AtRGS1-YFP and mCherry-AtREM1.3 were treated with ½ MS liquid medium (CK) and 100 μM MeJA (JA) for 8 h.