Additional file 3 of VA-TIRFM-based SM kymograph analysis for dwell time and colocalization of plasma membrane protein in plant cells

Additional file 3: Figure S1. SCI of AtRGS1-YFP or mCherry-AtREM1.3 analyzed by ImageJ software under different conditions. The 6-day-old transgenic seedlings expressing AtRGS1-YFP (A) and mCherry-AtREM1.3 (B) were treated with ½ MS liquid medium (CK) and 100 μM MeJA (JA) for 8 h by TIRFM observation. ***P < 0.001. ns > 0.05. Student’s t-test. Error bars represent the SD. n = 32 to 67 per treatment.