Additional file 5 of VA-TIRFM-based SM kymograph analysis for dwell time and colocalization of plasma membrane protein in plant cells

Additional file 5: Figure S3. Colocalization between AtRGS1-YFP and mCherry-AtREM1.3 analyzed by VA-TIRFM under different conditions. A–C Typical single-molecular viewer of AtRGS1-YFP (A), mCherry-AtREM1.3 (B), and merge (C) images at steady state. D–F Typical single-molecular viewer of AtRGS1-YFP (D), mCherry-AtREM1.3 (E), and merge (F) images upon JA treatment. Bar = 5 μm. The 6-day-old transgenic seedlings co-expressing AtRGS1-YFP and mCherry-AtREM1.3 were treated with ½ MS liquid medium (CK) and 100 μM MeJA (JA) for 8 h.