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3,201 results on '"Gupte A"'

1. Sex Differences in TB Clinical Presentation, Drug Exposure, and Treatment Outcomes in India

Author

Sona Deshmukh, Manasi Sane, Sanjay Gaikwad, Tushar Sahasrabudhe, Madhusudan Barthwal, Rahul Lokhande, Swapnil Raskar, Anju Kagal, Sujata Dharmshale, Neeta Pradhan, Akshay Gupte, Omamah Alfarisi, Amita Gupta, Kelly E. Dooley, Nikhil Gupte, Jonathan E. Golub, and Vidya Mave

Subjects
Pulmonary and Respiratory Medicine, Cardiology and Cardiovascular Medicine, Critical Care and Intensive Care Medicine
Published
2023

2. Scaffold-associated procedures are superior to microfracture in managing focal cartilage defects in the knee: A systematic review & meta-analysis

Author

Seewoonarain, S, Ganesh, D, Perera, E, Popat, R, Jones, J, Sugand, K, and Gupte, C

Subjects
Orthopedics and Sports Medicine
Abstract

BACKGROUND: Debate continues as to whether surgical treatment with chondral-regeneration devices is superior to microfracture for focal articular cartilage defects in the knee. PURPOSE: To evaluate the superiority of scaffold-associated chondral-regeneration procedures over microfracture by assessing: (1) Patient-reported outcomes; (2) Intervention failure; (3) Histological quality of cartilage repair. STUDY DESIGN: A three-concept keyword search strategy was designed, in accordance with PRISMA guidelines: (i) knee (ii) microfracture (iii) scaffold. Four databases (Ovid Medline, Embase, CINAHL and Scopus) were searched for comparative clinical trials (Level I-III evidence). Critical appraisal used two Cochrane tools: the Risk of Bias tool (RoB2) for randomized control trials and the Risk of Bias in Non-randomized Studies-of Interventions (ROBINS-I). Study heterogeneity permitted qualitative analysis with the exception of three patient-reported scores, for which a meta-analysis was performed. RESULTS: Twenty-one studies were identified (1699 patients, age range 18-66 years): ten randomized control trials and eleven non-randomized study interventions. Meta-analyses of the International Knee Documentation Committee (IKDC), Knee Injury And Osteoarthritis Outcome Score (KOOS) for pain and activities of daily living, and Lysholm score demonstrated statistically significant improvement in outcomes for scaffold procedures compared to microfracture at two years. No statistical difference was seen at five years. CONCLUSION: Despite the limitations of study heterogeneity, scaffold-associated procedures appear to be superior to MF in terms of patient-reported outcomes at two years though similar at five years. Future evaluation would benefit from studies using validated clinical scoring systems, reporting failure, adverse events and long-term clinical follow up to determine technique safety and superiority.

Published
2023

3. Comparative Evaluation Of The Effectiveness Of Dexamethasone And Adrenaline With 2% Lignocaine For Pterygomandibular Nerve Blocks: A Randomized Controlled Clinical Study

Author

Rinku Kalra, Ranjana S Patnaik, Shreyas Gupte, Nitin Bapu Jadhav, Mangal More, and Manthan Mehta

Subjects
General Medicine
Abstract

Background and Aim: Dexamethasone, a glucocorticoid, has been used in local anesthetics as an additive for various locoregional nerve blocks. Although, its use in the dental field has not been unexplored and concealed. The current study was conducted to evaluate and compare the calibre of anesthesia, their hemodynamic response, vasoconstrictive effects, and control of pain while administering pterygomandibular nerve blocks using 2% Lignocaine hydrochloride solution with Dexamethasone(4mg/ml) with that of the standard 2% Lignocaine hydrochloride solution and Adrenaline bitartrate (1:80000). Materials and Methods: This triple-blind randomized controlled study included 80 healthy subjects, aged between 18-45 years with an indication for surgical removal of impacted mandibular third molars. With the aid of a computer-generated sequence, the subjects were arbitrarily assigned into 2 groups (40/group)- Group 1: patients administered with 2% Lignocaine hydrochloride and Dexamethasone Sodium Phosphate (4mg/ml) solution, and Group 2: patients administered with 2% Lignocaine hydrochloride with Adrenaline bitartrate (Adr) 1: 80,000 (12.5 μgm/ml) solution. The variables were assessed based on their time of onset, depth (pain) and duration of anesthesia, systolic, diastolic, and mean arterial blood pressures, heart rate, and blood loss. Results: Statistically significant difference (P0.05) for pain, blood loss, cardiovascular variables (systolic, diastolic and mean arterial blood pressures and heart rate) at various intervals was noted. Conclusion: Dexamethasone added to Lignocaine shortens onset, prolongs duration of action and yields better control of post-operative pain as compared to Lignocaine with Adr. Vasoconstrictive properties, hemodynamic variables and intra-op pain control were comparable. Bangladesh Journal of Medical Science Vol.22 (Special Issue) 2023 p.172-178

Published
2023

4. Characterization of pigment produced by high carotenoid yielding bacteria Paracoccus marcusii RSPO1 and evaluation of its anti-diabetic, anti-microbial and antioxidant properties

Author

Naik, Raj and Gupte, Shilpa

Subjects
Organic Chemistry, Plant Science, Biochemistry, Analytical Chemistry
Abstract

High pigment producing bacteria was isolated and identified as Paracoccus marcusii RSPO1 using biochemical and 16s rRNA identification. Bacterial pigment production was optimised using parameters like inoculum size, nitrogen source, pH, temperature, and agitation speed. Carotenoids production was 7,240 ± 41 µg L−1 after optimization. The silica column purified pigment was characterized using UV-visible spectroscopy, TLC, FTIR, LC-ESI-MS and NMR, which revealed its composition as astaxanthin, zeaxanthin, ζ-carotene and β-zeacarotene. The inhibition assays against α-amylase and α-glucosidase showed IC50 values as 226 µg ml−1 and 0.7548 µg ml−1 respectively. The MIC of 1000 µg ml−1 of carotenoid was found to be effective against Escherichia coli and Enterobacter aerogenes when tested for antibacterial activity. Moreover, antioxidant activity of carotenoid sample was also determined where antioxidant potential of extracted carotenoid for DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) inhibition were 65 ± 0.06% and 42 ± 07% respectively at 20 µg ml−1 concentration.

Published
2023

5. The Effectiveness and Flexibility of Practice-Based Teaching: A Long-term, Cross-disciplinary Evaluation

Author

Jacey A. Greece, Candice Bangham, James Wolff, Malcolm Bryant, Gouri Gupte, and Donna McGrath

Subjects
Schools, Public Health, Teaching, Public Health, Environmental and Occupational Health, Administrative Personnel, Humans, Public Health, Students, Faculty
Abstract

Objectives: Schools of public health (SPHs) must train students through real-world situations. Practice-based teaching (PBT) uses immediate application for skill development through multiple modes of delivery including virtual collaborations. PBT provides short-term benefits but is less understood long term. We sought to understand the longer-term impacts of PBT on students, public health agencies, faculty, and SPH administration and determine if the reported benefits of PBT outweigh the challenges. Methods: We used a mixed-methods pre–post-test evaluation to assess short-term outcomes in spring 2015 and long-term outcomes 3 years later using quantitative and qualitative assessments. The evaluation included 4 PBT courses spanning disciplines in 1 SPH. Participants included students (n = 56), collaborating agencies (n = 9), teaching faculty (n = 7), and SPH administrators (n = 8). Results: Students reported acquisition of competencies, application at follow-up, and being workforce ready (91.0%) with greater appreciation 3 years post-semester (78.6%). Collaborating agencies reported successful implementation of deliverables (77.8%) and enhanced networks (88.7%) with cost savings and better outcomes for the communities served. Faculty recognized beneficial outcomes to students including workforce preparation (71.4%), and administration recognized the benefits and worth of PBT to the school for teaching (100%) and research opportunities (87.5%). Conclusions: Given the shifting higher education landscape as a result of the pandemic, public health courses are being redesigned to provide opportunities for skill development, regardless of delivery mode, and enhanced connections to the field are essential. PBT is a flexible pedagogy that results in lasting and innovative resources to agencies and prepares public health students with technical skills and professional competencies to be workforce ready.

Published
2024

10. Abstract P1-08-06: Oncology nursing in India: are we up to speed?

Author

Garvit Chitkara, Sridevi Murali-Nanavati, Nikhil Bardeskar, Ajinkya Gupte, Shruti Behal, Mugdha Lad, Prajakta Dongarkar, Muzammil Shaikh, and Kaustav Talapatra

Subjects
Cancer Research, Oncology
Abstract

Background: Cancer management is a critical component of healthcare worldwide. Improvement in cancer care has improved the longevity of patients, and they require continued care from healthcare workers, including nurses. Although nurses in India are trained to care for patients in general, courses dedicated to cancer care are few. The present study evaluated the knowledge of nurses involved in cancer management via an online survey. Materials and methods: A survey pertaining to nursing practices in cancer management was distributed electronically to nurses in India from January to April 2022. The survey was designed to evaluate treatment-specific knowledge and confidence of the nurses in managing patients with cancer. The data received was analyzed using Microsoft Excel 2016. The responses were divided into two groups: responses marked 4 and 5 on the scale were grouped as confident responses, whereas those marked 1, 2, and 3 were grouped as unconfident responses. Chi-squared analyses were performed using the OpenEpi online tool. Results: A total of 422 nurses replied to the questionnaire; of these, 399 (94.55%) said that they have experience in caring for patients with cancer. Of the 399, 198 (49.62%) worked in specialized cancer care centers and the remaining 201 (50.38%) worked in general hospitals. Nearly two-third of the nurses (n = 262; 65.7%) replied that they studied cancer care as a part of their nursing curriculum, whereas the remaining learnt through personal experience. A total of 335 (84%) nurses had undergone a specialized course in chemotherapy management, and most were confident about their knowledge on chemotherapy administration, central line management, and side effect and precaution management. Only 41 (10.3%) had undergone a specialized course in oncology-directed surgical management; yet based on their primary nursing training more than half of the nurses were confident of performing pre- and postoperative management. With respect to a more specialized surgical care, only 144 (36.1%) were confident in providing stoma care and 181 (45.4%) were confident in teaching preventive lymphedema care after surgery. Although a majority had completed a specialized course in post-radiation management (n = 255; 93.2%), only half were confident in identifying skin-induced changes and providing care for patients receiving radiotherapy. Furthermore, only half of the nurses were aware of the role of nursing staff in brachytherapy (n = 200; 50.1%). Most nurses did not undergo any specialized course (n = 360; 90.2%) in palliative management; 124 (31.1%) nurses said that they were not very confident in adequately counselling patient relatives with regard to symptomatic treatment. Nurses working in specialized cancer centers (n = 198) were significantly more confident in administering and managing central lines for chemotherapy; performing adequate chemotherapy drug disposal; and assessing the needs of cancer patients and their family members than their counterparts working in general hospitals (n = 201) (all p < 0.05). Only 54.4% of the respondents were aware of oncology-directed nursing programs available in the country, and only half were able to access them (n = 160; 55.2%) owing to the lack of guidance (n = 130; 32.58%), time (n = 93; 23.31%), and funds (n = 46; 11.53%). Hence, when asked whether they would be inclined to attend a hybrid course, majority (n = 349; 87.47%) indicated in the affirmative. Also, 338 (84.7%) nurses replied that they would be inclined to participate in cancer screening programs after pursuing such a specialized course. Conclusions: The results of this study highlight the lacunae in nurses’ oncology training. Thus, there is an obvious need to redesign the existing oncology-directed nursing programs to include palliative care and organ-site specific care. Designing these courses in the hybrid format may improve its accessibility and the willingness of nurses to attend. Citation Format: Garvit Chitkara, Sridevi Murali-Nanavati, Nikhil Bardeskar, Ajinkya Gupte, Shruti Behal, Mugdha Lad, Prajakta Dongarkar, Muzammil Shaikh, Kaustav Talapatra. Oncology nursing in India: are we up to speed? [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr P1-08-06.

Published
2023

13. Development of a Data Analysis Module

Author

Atharva Gupte, Kaushal Bhide, Swarupa Deshpande, Manas Apte, Rohan Rasane, and Atharva Gupte

Subjects
Model Building, Data Collection, Data Analysis, Data Pre-processing, Extract, Transform, Load, SQL Database, API, DLL, NoSQL, Data Aggregation, OOP, Script Generation
Abstract

A module that gathers data from various sources like SQL databases or CSVs and then, with the help of this data, provides meaningful insights to the customer. The software will be an efficient way to track data from varied sources in real time. It will provide a centralized system to monitor and analyze the performance of any business/organization. With the help of this software, or dashboard an environment can be created for business analysis as well as management. This module includes all phases of the Data Analysis life cycle, like data collection, data preprocessing, data analysis, visualization, and eventually effective decision making. A holistic solution for each step is given by the software so as to yield as many insights as possible. In today's time where data is the new currency, this software or module or dashboard will provide users with a wide range of options to work with and around data. With the help of this software, one can achieve effective monitoring and evaluation of the business sector. Due to the lack of such software, the available raw data is often not transformed and used for decision-making. To fill this void, this module will play a vital role. The software will provide customers with the options to check factors like tracking progress towards a set target, effective decision-making for planning, and predicting sector trends and performances. The customers will have autonomy to work with variable sizes and types of data. Propagating the results is also an important thing for the customers; therefore, the module or dashboard provides effective data visualization tools as well. Thus, this software is defined as an end-to-end solution for the customers.

Published
2023

14. Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Author

Global Health Research Unit on Global Surgery, National Institute for Health and Care Research, Adisa, Adewale, Bahrami-Hessari, Michael, Bhangu, Aneel, George, Christina, Ghosh, Dhruv, Glasbey, James, Haque, Parvez, Ingabire, J C Allen, Kamarajah, Sivesh Kathir, Kudrna, Laura, Ledda, Virginia, Li, Elizabeth, Lillywhite, Robert, Mittal, Rohin, Nepogodiev, Dmitri, Ntirenganya, Faustin, Picciochi, Maria, Simões, Joana Filipa Ferreira, Booth, L, Elliot, R, Kennerton, A S, Pettigrove, K L, Pinney, L, Richard, H, Tottman, R, Wheatstone, P, Wolfenden, John W D, Smith, A, Sayed, A Emad, Goswami, Aakansha Giri, Malik, Aamer, Mclean, Aaron Lawson, Hassan, Abbas, Nazimi, Abd Jabar, Aladna, Abdallah, Abdelgawad, Abdalrahman, Saed, Abdel, Abdelmageed, Abdelfatah, Ghannam, Abdelilah, Mahmoud, Abdelrahman, Alvi, Abdul, Ismail, Abdulaziz, Adesunkanmi, Abdulhafiz, Ebrahim, Abdulla, Al-Mallah, Abdullah, Alqallaf, Abdullah, Durrani, Abdullah, Gabr, Abdullah, Kirfi, Abdullahi Musa, Altaf, Abdulmalik, Almutairi, Abdulmjeed, Sabbagh, Abdulrahman J, Ajiya, Abdulrazak, Haddud, Abdurrahman, Alnsour, Abed Alfattah Mahmoud, Singh, Abhinav, Mittal, Abhishek, Semple, Abigail, Adeniran, Abiodun, Negussie, Abraham, Oladimeji, Abraham, Muhammad, Abubakar Bala, Yassin, Abubaker, Gungor, Abuzer, Tarsitano, Achille, Soibiharry, Adaiah, Dyas, Adam, Frankel, Adam, Peckham-Cooper, Adam, Truss, Adam, Issaka, Adamu, Ads, Adel Mohamed, Aderogba, Adeleke Akeem, Adeyeye, Ademola, Ademuyiwa, Adesoji, Sleem, Adham, Papa, Adrian, Cordova, Adriana, Appiah-Kubi, Adu, Meead, Adullah, Nacion, Aeris Jane D, Michael, Afieharo, Forneris, Agustin Albani, Duro, Agustin, Gonzalez, Agustin Rodriguez, Altouny, Ahmad, Ghazal, Ahmad, Khalifa, Ahmad, Ozair, Ahmad, Quzli, Ahmad, Haddad, Ahmad, Othman, Ahmad Faidzal, Yahaya, Ahmad Shuib, Elsherbiny, Ahmed, Nazer, Ahmed, Tarek, Ahmed, Abu-Zaid, Ahmed, Al-Nusairi, Ahmed, Azab, Ahmed, Elagili, Ahmed, Elkazaz, Ahmed, Kedwany, Ahmed, Nuhu, Ahmed Mohammed, Sakr, Ahmed, Shehta, Ahmed, Shirazi, Ahmed, Mohamed, Ahmed Mohamed Ibrahim, Sherif, Ahmed Elshawadfy, Awad, Ahmed K, Abbas, Ahmed M, Abdelrahman, Ahmed Saber, Ammar, Ahmed Siddique, Azzam, Ahmed Y, Ciftci, Ahmet Burak, Dural, Ahmet Cem, Sanli, Ahmet Necati, Rahy-Martín, Aida Cristina, Tantri, Aida Rosita, Khan, Aimal, Al-Touny, Aiman, Tariq, Aiman, Gmati, Aimen, Costas-Chavarri, Ainhoa, Auerkari, Aino, Landaluce-Olavarria, Aitor, Puri, Ajay, Radhakrishnan, Ajay, Ubom, Akaninyene Eseme, Pradhan, Akhilesh, Turna, Akif, Adepiti, Akinfolarin, Kuriyama, Akira, Kassam, Al-Faraaz, Hassouneh, Ala, El-Hussuna, Alaa, Habeebullah, Alaa, Ads, Alaa Mohamed, Mousli, Alaa, Biloslavo, Alan, Hoang, Alan, Kirk, Alan, Santini, Alasdair, Melero, Alba Vazquez, Calvache, Albaro José Nieto, Baduell, Albert, Chan, Albert, Abrate, Alberto, Balduzzi, Alberto, Sánchez, Alberto Cabañero, Navarrete-Peón, Alberto, Porcu, Alberto, Brolese, Alberto, Barranquero, Alberto G, Saibene, Alberto Maria, Adam, Albushra Altayeb, Vagge, Aldo, Maquilón, Alejandra Jara, Leon-Andrino, Alejandro, Sekulić, Aleksandar, Trifunovski, Aleksandar, Mako, Aleksandro, Bedada, Alemayehu Ginbo, Broglia, Alessandro, Coppola, Alessandro, Giani, Alessandro, Grandi, Alessandro, Iacomino, Alessandro, Moro, Alessandro, D’amico, Alessia, Malagnino, Alessia, Tang, Alethea, Doyle, Alex, Alfieri, Alex, Haynes, Alex, Wilkins, Alex, Baldwin, Alexander, Heriot, Alexander, Laird, Alexander, Lazarides, Alexander, O'connor, Alexander, Trulson, Alexander, Rokohl, Alexander Christopher, Caziuc, Alexandra, Triantafyllou, Alexandra, Anesi, Alexandre, Nikova, Alexandrina, Andrianakis, Alexandros, Charalabopoulos, Alexandros, Tsolakidis, Alexandros, Chirca, Alexandru, Arnaud, Alexis P, Narvaez-Rojas, Alexis Rafael, Kavalakat, Alfie, Spina, Alfio, Recordare, Alfonso, Annicchiarico, Alfredo, Conti, Alfredo, Mohammed, Alhassan Datti, Kocataş, Ali, Almhimid, Ali, Arnaout, Ali, Fahmy, Ali, Mangi, Ali, Modabber, Ali, Ulas, Ali Bilal, Mohamedahmed, Ali Yasen Y, Frontali, Alice, Moynihan, Alice, Yunus, Alif, Ahmad, Aline, Kent, Alistair J, Khamees, Almu'atasim, Ugwu, Aloy Okechukwu, Turan, Alparslan, Mohammed, Alsnosy Abdullah Khalefa, Navarro-Barrios, Alvaro, Yebes, Alvaro, De Sousa, Álvaro Francisco Lopes, Moreno, Amabelle, Sethi, Aman, Dawson, Amanda Caroline, Othman, Amani Alsayd Abdulsalam, Kaur, Amanjot, Wolde, Amanuel, Antonelli, Amedeo, Scifo, Amedeo, Alhamad, Ameen, Davis, Amelia, Alderazi, Amer, Harky, Amer, Mohammed-Durosinlorun, Amina, Seguya, Amina, Okhakhu, Amina, Chamakhi, Amine, Sebai, Amine, Souadka, Amine, Asla, Amir, Agrawal, Amit, Persad, Amit, Gupta, Amit, Elgazar, Amr, Kulkarni, Amruta, Coates, Amy, Bellés, Ana Ciscar, Hadzibegovic, Ana Danic, Jotic, Ana, Kowark, Ana, Martins, Ana, Pineda, Ana Mejía, Peral, Ana, Gollarte, Ana Sánchez, Senent-Boza, Ana, Camarena, Ana Isabel Avellaneda, Castaño-Leon, Ana M, Bravo, Ana Maria Minaya, Moro, Ana María Gimeno, Musina, Ana-Maria, Tapia-Herrero, Ana-María, Kothari, Anai, Gupta, Anand, Raja, Anand, Aljaiuossi, Anas, Taha, Anas, Majbar, Anass Mohammed, Prodromidou, Anastasia, Kanatas, Anastasios, Gupte, Anaya, Zakaria, Andee Dzulkarnaen, Balla, Andrea, Barberis, Andrea, Bondurri, Andrea, Bottari, Andrea, Costantino, Andrea, Figus, Andrea, Lauretta, Andrea, Mingoli, Andrea, Romanzi, Andrea, Sagnotta, Andrea, Scacchi, Andrea, Picchetto, Andrea, Valadez, Andrea Estefania Cueto, Luzzi, Andrea-Pierre, Älgå, Andreas, Fontalis, Andreas, Hecker, Andreas, Demetriades, Andreas K, Serban, Andreea-Madalina, Văcărașu, Andrei Bogdan, Cokan, Andrej, Isaza-Restrepo, Andres, Beamish, Andrew, Schache, Andrew, Stevenson, Andrew, Yiu, Andrew, Cockbain, Andrew J, Litvin, Andrey, Abad-Motos, Ane, Becerra, Angel, Ramos, Ángel Cilleruelo, Chiaradia, Angela, Dell, Angela, Romano, Angela, Pascale, Angelo, Marra, Angelo Alessandro, Dimas, Angelos, Kolias, Angelos, Cerovac, Anis, Koneru, Anish, Tidjane, Anisse, Agbeko, Anita Eseenam, Bajaj, Ankur, Gosain, Ankush, Allan, Anna, Carreras-Castañer, Anna, D'amore, Anna, Dare, Anna, Maffioli, Anna, Palepa, Anna, Paspala, Anna, Konney, Anna, Gatta, Anna Nunzia Della, Ezanno, Anne-Cecile, Yiallourou, Anneza, Kinnair, Anthony, Rayner, Anthony, Scafa, Anthony Kevin, Bowan, Antoinette Bediako, Veglia, Antonella, Russo, Antonino, Maniaci, Antonino, Castaldi, Antonio, Gil-Moreno, Antonio, Maffuz-Aziz, Antonio, Meola, Antonio, Nenna, Antonio, Ferrer, Antonio Perez, Bonilla, Antonio Ramos, Ramos-De La Medina, Antonio, Infante, Antonio Rodriguez, Santoro, Antonio, Laganà, Antonio Simone, Bateman, Antony, Michael, Antony Louis Rex, Abozid, Anwaar, Seidu, Anwar Sadat, Lowery, Aoife, Tantraworasin, Apichat, Rasheed, Aqsa, Picciariello, Arcangelo, Isik, Arda, Saif, Areeba, Anjum, Arfa, Ioannidis, Argyrios, Abeldaño, Ariel, Hussain, Arif, Nathan, Arjun, Bedzhanyan, Arkady, Perfecto, Arkaitz, De Virgilio, Armando, Galvan, Armando, Sablotzki, Armin, Böttcher, Arne, Pellacani, Arrigo, Gatti, Arthur, Ibrahimli, Arturan, Menon, Arun, Sahni, Arun, Mwenda, Aruyaru Stanley, Choudhry, Asad, Jayawardane, Asanka, Gupta, Ashish, Ramasamy, Ashok, Mitul, Ashrarur Rahman, Bawa, Ashvind, Nugur, Ashwani, Rammohan, Ashwin, Sachdeva, Ashwin, Mehraj, Asif, Yildirim, Asif, Alqaseer, Asma, Radwan, Asmaa, Sallam, Asser, Syllaios, Athanasios, Tampakis, Athanasios, Alwael, Athari, Samara, Athina, Eroglu, Atilla, Rahman, Atiqur, Ulkucu, Attila, Zaránd, Attila, Dulskas, Audrius, Tawiah, Augustine, Zani, Augusto, Vas, Aurelia, Lukosiute-Urboniene, Ausra, Adamu, Auwal, Aujayeb, Avinash, Malik, Awais Amjad, İplikçi, Ayberk, Mahmud, Ayesha, Cil, Aylin Pelin, Makanjuola, Ayomide, Akwaisah, Ayoub, Galandarova, Aysuna, Saracoglu, Ayten, Regan, Azel, Barlas, Aziz Mutlu, Alhassan, Baba Alhaji Bin, Mostafa, Badr, Hamida, Baha Ben, Torun, Bahar Canbay, Abdullah, Bahiyah, Balagobi, Balasingam, Banky, Balazs, Singh, Baljit, Alegbeleye, Bamidele, Yigit, Banu, Hajjaj, Baraa Nabil, Burgos-Blasco, Barbara, Seeliger, Barbara, Alayande, Barnabas, Alhazmi, Barrag, Enodien, Bassey, Torre, Beatrice, Pérez, Beatriz Gómez, Tamayo, Beatriz Villota, De Andrés-Asenjo, Beatriz, Quintana-Villamandos, Begoña, Girgin, Behiç, Barmayehvar, Behrad, Beisenov, Bekzat, Creavin, Ben, Dunne, Ben, Marson, Ben, Waterson, Ben, Martin, Benjamin, Zucker, Benjamin, Wong, Benjamin Ngie Xiong, Ozmen, Berk Baris, Hammond, Bernard, Mbwele, Bernard, Núñez, Bernardo, Dhondt, Bert, Gafsi, Besma, Mcleish, Bethan, Lieske, Bettina, Tailor, Bhavesh, La Pira, Biagia, Picardi, Biagio, Zampogna, Biagio, Casagranda, Biagio, Festa, Bianca Maria, Panda, Bijnya, Kirmani, Bilal, Sulaiman, Bilal, Gurung, Binay, Zacharia, Bincy, Bette, Birgit, Ayana, Birhanu, Nikolovska, Bisera, Vilaró, Blanca Capdevila, De Vega Sánchez, Blanca, Hameed, Bm Zeeshan, Diaconescu, Bogdan, Kovacevic, Bojan, Bumber, Boris, Sakakushev, Boris, Tadic, Boris, Malek, Bouhani, Alrayes, Bourhan, Thomas, Bradley, Gális, Branislav, Gallagher, Brendan, Knowles, Brett, Cunningham, Brian, Daley, Brian, Mishra, Brijesh, Ashford, Bruce, Pirozzi, Brunella Maria, Berselli, Bruno, Martinez-Leo, Bruno, Sensi, Bruno, Nardo, Bruno, Celik, Burak, Giray, Burak, Abud, Burçin, Almiqlash, Bushray, Pramesh, C S, Taskiran, Cagatay, De Campos Prado, Caio Antonio, Cipolla, Calogero, Kumar, Calvin, English, Camilla, Riccetti, Camilla, Vanni, Camilla, Brasset, Camille, Downey, Candice, Duffy, Caoimhe, Chwat, Carina, Cutmore, Carina, Sars, Carl, Ratto, Carlo, Pacilio, Carlo Alberto, De La Infiesta García, Carlos, Moreno, Carlos Guijarro, Magalhães, Carlos, Prada, Carlos, Zapata, Carlos Shiraishi, Senni, Carlotta, Flumignan, Carolina Dutra Queiroz, Martinez-Perez, Carolina, Duarte, Carolina Lugo, Garcia, Carolina Soledad Romero, Anderson, Caroline, Hing, Caroline, Cullinane, Carolyn, Cina, Caterina, Zabkiewicz, Catheriner, Sohrabi, Catrin, Guldogan, Cem Emir, Ciubotaru, Cezar, Desai, Chaitya, Raut, Chandrajit, Demetriou, Charis, Handford, Charles, Okpani, Charles, Paranjape, Charudutt, Koh, Cherry, Khatri, Chetan, Parmar, Chetan, Mok, Chi Wei, Caricato, Chiara, Marafante, Chiara, Echieh, Chidiebere Peter, Tan, Chih Ying, Ong, Chon Sum, Conso, Christel, Jardinez, Christiaan, Konrads, Christian, Warner, Christian, Makwe, Christian Chigozie, Henein, Christin, Fleming, Christina, Roland, Christina L, Maurus, Christine, Nitschke, Christine, Mittermair, Christof, Mallmann, Christoph, Andro, Christophe, Harmston, Christopher, Kuppler, Christopher, Lotz, Christopher, Nahm, Christopher, Rowe, Christopher, Ryalino, Christopher, Wallis, Christopher, Millward, Christopher Paul, Anthoulakis, Christos, Apostolou, Christos, Chouliaras, Christos, Kalfountzos, Christos, Kaselas, Christos, Vosinakis, Christos, Okereke, Chukwuma, Chean, Chung Shen, Barlow, Ciaran, Tatar, Cihad, Clancy, Cillian, Forde, Cillian, Sharpin, Claire, Mccarthy, Claire, Nestor, Claire, Warden, Claire, Ávila, Clara Castro, Massaguer, Clara, Fang, Clarissa Ern Hui, Martins, Claudia Pinto, Guerci, Claudio, Mauriello, Claudio, Holzmeister, Clemens, Miller, Clemens, Weber, Clemens, Wiesinger, Clemens Georg, Kenington, Cleo, Noel, Colin, Sue-Chue-Lam, Colin, Adumah, Collins, Neary, Colm, Sen, Comert, Fitzgerald, Conall, Ezeme, Constantine, Nastos, Constantinos, Mesina, Cristian, Bombardini, Cristina, Torregrosa, Cristina, Valdespino, Cristina Puentes, Don, Cristine Pathirannehalage, Wickramasinghe, Dakshitha, Milanesi, Damiano, Armijos, Daniel, Asiimwe, Daniel, Beswick, Daniel, Clerc, Daniel, Cox, Daniel, Doherty, Daniel, Martínez, Daniel Fernández, Lechuga, Daniel Garay, Gero, Daniel, Gil-Sala, Daniel, Lindegger, Daniel, Reim, Daniel, Shaerf, Daniel, Shmukler, Daniel, Branzan, Daniela, Filipescu, Daniela, Rega, Daniela, Bernardi, Daniele, Bissacco, Daniele, Fusario, Daniele, Morezzi, Daniele, Sabella, Danielle, Zimak, Danijela Mrazovac, Vinci, Danilo, Sale, Danjuma, Khan, Danyal Zaman, Thereska, Dariel, Andreotti, Dario, Tartaglia, Dario, Abdulai, Darling Ramatu, Mukherjee, Dattatreya, Verdi, Daunia, Idowu, 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Prashanth, Salunke, Pravin, Shah, Pritik A, Punjabi, Prof Prakash P, Christodoulou, Prokopi, Hamdan, Qusai, Tawalbeh, Ra'fat, Gadelkareem, Rabea, Awad, Rabih, Callcut, Rachael, Clegg, Rachael, Choron, Rachel, Payne, Rachel, Gefen, Rachel, Costea, Radu, Drasovean, Radu, Mirica, Radu Mihail, Ravindra, Raevin, Fajardo, Rafael Torre, Nunes, Rafael Leite, Aspide, Raffaele, Lombardi, Raffaele, Vidya, Raghavan, Elboraei, Rahma, Saaid, Rahmah, Ghodke, Rahul, Gupta, Rahul, Sharma, Rahul Deo, Lunevicius, Raimunda, Kalayarasan, Raja, Mohan, Rajashekar, Singh, Rajdeep, Sivaprakasam, Rajesh, Seenivasagam, Rajkumar Kottayasamy, Rajendram, Rajkumar, Radulescu, Raluca Bievel, Goicea, Raluca, Seshadri, Ramakrishnan Ayloor, Sarı, Ramazan, Nataraja, Ramesh, Aslam, Ramisha, Abdelemam, Rania, Shrestha, Rashmit, Bharathan, Rasiah, Pellini, Raul, Guevara, Raul, Agarwal, Ravi, Vissapragada, Ravi, Alharmi, Rawan Arahman, Sayyed, Raza, Browning, Rebecca, Critchley, Rebecca, Mallick, Rebecca, Alarabi, Rehab, Beron, Reinaldo Isaac, Függer, Reinhold, Othman, Rema, Saad, Rema, Amores, Remedios Revilla, Colombari, Renan Carlo, Radivojević, Renata Curic, Patrone, Renato, Novysedlák, René, Huatuco, René Manuel Palacio, Baertschiger, Reto, Liang, Rhea, Luckwell, Rhy, Escrevente, Ricardo, Rezende, Ricardo Fernande, Cruz, Ricardo Pedrini, Lenzi, Riccardo, Rosati, Riccardo, Donovan, Richard, Egan, Richard, Morris, Richard, Page, Richard, Seglenieks, Richard, Unsworth, Richard, Wilkin, Richard, Skipworth, Richard Je, Davies, Richard Justin, Bezirci, Rifat, Talwar, Rishi, Azami, Rizwan, Bohmer, Rob, Crichton, Robert, Fruscio, Robert, Hooker, Robert, Jach, Robert, Parker, Robert, Pillerstorff, Robert, Sinnerton, Robert, Stabler, Robert, O'connell, Robert Michael, Ragozzino, Roberta, Tutino, Roberta, Angelico, Roberta, Cammarata, Roberto, Colasanti, Roberto, Macchiavello, Roberto, Peltrini, Roberto, Pirrello, Roberto, Vaschetti, Roberto, Pires, Robinson Esteve, Papalia, Rocco, Arrangoiz, Rodrigo, Hompes, Roel, Salah, Rokeya, Pinto, Romina, Flumignan, Ronald, Callan, Rory, Cuthbert, Rory, Dennis, Rory, Scaramuzzo, Rosa, Macías, Rosa Montero, Sánchez, Rosa, Ogu, Rosemary, Ramely, Rosnelifaizur, Sgarzani, Rossella, Ramli, Roszalina, Hillier, Roxane, Thumbadoo, Ruben, Ooi, Rucira, Abdus-Salam, Rukiyat, Masri, Ruqaya, Hodgson, Russell, Mathew, Ryan, Wade, Ryckie, D'archi, Sabatino, Khan, Sabina, Ngaserin, Sabrina, Kale, Sachin, Hassan, Sadiq, Merghani, Safa, Benamar, Safia, Muhammad, Sagir, Badran, Saif, Elsahli, Saifaleslam, Heta, Saimir, Hammouche, Salah, Baeesa, Saleh, Paiella, Salvatore, Eldeen, Samaher Taj Eldeen Hassan Taj, Arkani, Samara, Mittal, Samarth, Hirji, Sameer, Tebha, Sameer, Emile, Sameh, Dbouk, Samer, Bandyopadhyay, Samik Kumar, Muhammad, Saminu, Olori, Samson, Asirifi, Samuel Amoako, Hailu, Samuel, Ling, Samuel, Newman, Samuel, Ross, Samuel, Wanjara, Samuel, Kumar, Sandip, Seneviratne, Sanjeewa, Tamburello, Sara, Suarez, Sara Busto, Ingallinella, Sara, Irshaidat, 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Pazmiño, P, Coughlin, P, Kirchweger, P, Pessaux, P, Maguire, P, Petrone, P, Cullis, P, Köglberger, P, Marriott, P, Nankivell, P, Santos-Costa, P, Martins, P, Panahi, P, Botelho, P, Teixeira, P, Escobar, P, Vázquez, P, Gribnev, P, Nolte, P, Agbonrofo, P, Bobak, P, Choong, P, Elbe, P, Hutchinson, P, Labib, P, Paal, P, Pockney, P, Reemst, P, Szatmary, P, Vaughan-Shaw, P, Alexander, P, Pucher, P, Stather, P, Foessleitner, P, Winnand, P, Zehnder, P, Kruse, P, Matos, P, Lapolla, P, Cicerchia, P, Solli, P, Di Lascio, P, Zarif, P, Champagne, P, Anoldo, P, Bertoglio, P, Fransvea, P, Familiari, P, Lombardi, P, Stogowski, P, Bruzzaniti, P, Tripathi, P, D'Sa, P, Salunke, P, Shah, P, Punjabi, P, Christodoulou, P, Hamdan, Q, Tawalbeh, R, Gadelkareem, R, Awad, R, Callcut, R, Clegg, R, Choron, R, Payne, R, Gefen, R, Costea, R, Drasovean, R, Mirica, R, Ravindra, R, Fajardo, R, Nunes, R, Aspide, R, Lombardi, R, Vidya, R, Elboraei, R, Saaid, R, Ghodke, R, Gupta, R, Sharma, R, Lunevicius, R, Kalayarasan, R, Mohan, R, Singh, R, Sivaprakasam, R, Seenivasagam, R, Rajendram, R, Radulescu, R, Goicea, R, Seshadri, R, Sarı, R, Nataraja, R, Aslam, R, Abdelemam, R, Shrestha, R, Bharathan, R, Pellini, R, Guevara, R, Agarwal, R, Vissapragada, R, Alharmi, R, Sayyed, R, Browning, R, Critchley, R, Mallick, R, Alarabi, R, Beron, R, Függer, R, Othman, R, Saad, R, Amores, R, Colombari, R, Radivojević, R, Patrone, R, Novysedlák, R, Huatuco, R, Baertschiger, R, Liang, R, Luckwell, R, Escrevente, R, Rezende, R, Cruz, R, Lenzi, R, Rosati, R, Donovan, R, Egan, R, Morris, R, Page, R, Seglenieks, R, Unsworth, R, Wilkin, R, Skipworth, R, Davies, R, Bezirci, R, Talwar, R, Azami, R, Bohmer, R, Crichton, R, Fruscio, R, Hooker, R, Jach, R, Parker, R, Pillerstorff, R, Sinnerton, R, Stabler, R, O'Connell, R, Ragozzino, R, Tutino, R, Angelico, R, Cammarata, R, Colasanti, R, Macchiavello, R, Peltrini, R, Pirrello, R, Vaschetti, R, Pires, R, Papalia, R, Arrangoiz, R, Hompes, R, Salah, R, Pinto, R, Flumignan, R, Callan, R, Cuthbert, R, Dennis, R, Scaramuzzo, R, Macías, R, Sánchez, R, Ogu, R, Ramely, R, Sgarzani, R, Ramli, R, Hillier, R, Thumbadoo, R, Ooi, R, Abdus-Salam, R, Masri, R, Hodgson, R, Mathew, R, Wade, R, D'Archi, S, Khan, S, Ngaserin, S, Kale, S, Hassan, S, Merghani, S, Benamar, S, Muhammad, S, Badran, S, Elsahli, S, Heta, S, Hammouche, S, Baeesa, S, Paiella, S, Eldeen, S, Arkani, S, Mittal, S, Hirji, S, Tebha, S, Emile, S, Dbouk, S, Bandyopadhyay, S, Olori, S, Asirifi, S, Hailu, S, Ling, S, Newman, S, Ross, S, Wanjara, S, Kumar, S, Seneviratne, S, Tamburello, S, Suarez, S, Ingallinella, S, Irshaidat, S, Konswa, S, Mambrilla, S, Nasser, S, Parini, S, Pitoni, S, Ornaghi, S, Rodrigues, S, Abdelmohsen, S, Aitken, S, Tian, S, Badiani, S, Ahmad, S, Swed, S, Muthu, S, Lakpriya, S, Alzahrani, S, Mikalauskas, S, Lasrado, S, Satoskar, S, Bawa, S, Altiner, S, Garcia, S, Stevens, S, Demir, S, Ken-Amoah, S, Tranca, S, Ziemann, S, Awad, S, Atici, S, Subramaniam, S, Erel, S, Jiang, S, Efetov, S, Efremov, S, Katorkin, S, Valladares, S, Contreras, S, Meriç, S, Zenger, S, Safi, S, Leventoğlu, S, Elsalhawy, S, Shaikh, S, Sheik, S, Islam, S, Shamim, S, Waqar, S, Farid, S, Seraj, S, Sundarraju, S, Karandikar, S, Sambhwani, S, Chopra, S, Chowdhury, S, Laura, S, Ahmed, S, Wason, S, Tan, S, Fraser, S, Williams, S, Ghozy, S, Abdelmawgoud, S, Shehata, S, Sharma, S, Al-Touny, S, Ramzanali, S, Nah, S, Jansen, S, Rajan, S, Dindyal, S, Amin, S, Shoukrie, S, Karar, S, Patkar, S, Abdulsalam, S, Lin, S, Hegde, S, Fiorelli, S, Quaresima, S, Redondo, S, Palmisano, S, Ruggiero, S, Balogun, S, Cais, S, Cole, S, Federer, S, Le Roux, S, Ippoliti, S, Meneghini, S, Viola, S, Manfredelli, S, Novello, S, Gananadha, S, Mesli, S, Tani, S, Malik, S, Anastasiadou, S, Boligo, S, Esposito, S, Valanci, S, Xenaki, S, Pejkova, S, Trungu, S, Basu, S, Alkhatib, S, Pérez-Bertólez, S, Flores, S, Donoghue, S, Lunca, S, Orsoo, S, Potamianos, S, Devarakonda, S, Suresh, S, Croghan, S, Turi, S, Capella, S, Lucchini, S, Magnone, S, Salizzoni, S, Scabini, S, Scaringi, S, Cioffi, S, Seyfried, S, Degener, S, Potten, S, Taha-Mehlitz, S, Ali, S, Angamuthu, S, Mcaleer, S, Knight, S, White, S, Mantziari, S, Kykalos, S, Goh, S, Ibrahim, S, Elzwai, S, Bansal, S, Tripathy, S, Amrayev, S, Anwar, S, Banerjee, S, Thakar, S, Saeed, S, Venkatappa, S, Das, S, Techapongsatorn, S, Dube, S, Lee, S, González-Suárez, S, Henriques, S, Konjevoda, S, Gisbertz, S, Bravo, S, Mannan, S, Bukhari, S, Zafar, S, Batista, S, Chin, S, Arif, T, Lawal, T, Aktokmakyan, T, Osborn, T, Szakmany, T, Sztipits, T, Triantafyllou, T, Valadez, T, Singh, T, Khaliq, T, Patel, T, Fadalla, T, Jichi, T, Sammour, T, Al-Shaiji, T, Naggs, T, Barišić, T, Nikolouzakis, T, Bisgin, T, Perra, T, Uprak, T, Dagklis, T, Liakakos, T, Sidiropoulos, T, Adjeso, T, Dölker, T, Oung, T, Aherne, T, Diehl, T, Pinkney, T, Raymond, T, Rhomberg, T, Schmitz-Rixen, T, Madhuri, T, Lohmann, T, Yeoh, T, Zaimis, T, Bright, T, Vilz, T, Glowka, T, Board, T, Hardcastle, T, Cohnert, T, Mahečić, T, William, T, Klatte, T, Abbott, T, Watcyn-Jones, T, Mendes, T, Kulis, T, Sečan, T, Campagnaro, T, Frisoni, T, Simoncini, T, Violante, T, Safranovs, T, Risteski, T, Pang, T, Akinyemi, T, Yotsov, T, Laeke, T, Kochiyama, T, Sholadoye, T, Alekberli, T, Ezomike, U, Giustizieri, U, Grossi, U, Köksoy, Ü, Bork, U, Kisser, U, Ronellenfitsch, U, Saeed, U, Bracale, U, Jayarajah, U, Rauf, U, Bumbasirevic, U, Ferrer, U, Ahmed, U, Bello, U, Jogiat, U, Sadia, U, Galandarov, V, Narayanan, V, Calu, V, Bianchi, V, Ciniero, V, Tonini, V, Silvestri, V, Vijay, V, Dewan, V, Lohsiriwat, V, Thuduvage, V, Mousafeiris, V, Dragisic, V, Sasireka, V, Santric, V, Kusuma, V, Kolli, V, Alonso, V, De Simone, V, Picotti, V, Martínez, V, Panduro-Correa, V, Kakotkin, V, Angulo, V, Turrado-Rodriguez, V, Krishnamoorthy, V, Ban, V, Shah, V, Maiola, V, Giordano, V, La Vaccara, V, Lizzi, V, Papagni, V, Schiavone, V, Satchithanantham, V, Garcia-Virto, V, Jimenez, V, Kumar, V, Shelat, V, Bhat, V, Sodhai, V, Graziadei, V, Kutuzov, V, Stoyanov, V, Oktseloglou, V, Flis, V, Elhassan, W, Yang, W, Soon, W, Tashkandi, W, Al-Khyatt, W, Mabood, W, Bijou, W, Wijenayake, W, D, W, Krawczyk, W, Atkins, W, Bolton, W, White, W, Ceelen, W, Vagena, X, Gozal, Y, Baba, Y, Subramani, Y, Jansen, Y, Mittal, Y, Kara, Y, Zwain, Y, Noureldin, Y, Alawneh, Y, Aydin, Y, Lam, Y, Tang, Y, Lim, Y, Dean, Y, Tanas, Y, Su, Y, Fujimoto, Y, Altinel, Y, Frolova, Y, Oshodi, Y, Fadel, Z, Zahid, Z, Elahi, Z, Djama, Z, Zaheen, Z, Jawad, Z, Demetrashvili, Z, Gebremeskel, Z, Gudisa, Z, Alyami, Z, Garoufalia, Z, Li, Z, Zimak, Z, Radin, Z, and Balogh, Z

Subjects
general surgery, acute care surgery, Settore MED/18 - CHIRURGIA GENERALE, climate change, trauma, Settore MED/40 - Ginecologia E Ostetricia, environmental impact, surgery, Settore MED/31 - Otorinolaringoiatria, None, Medicine and Health Sciences, Surgery, operating theatre, environment
Abstract

Background: Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods: This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low-middle-income countries. Results: In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of 'single-use' consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low-middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion: This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high- and low-middle-income countries.

Published
2023

15. Global leprosy scenario

Author

Mohan Gupte

Subjects
General Medicine, General Biochemistry, Genetics and Molecular Biology
Published
2023

16. Association of maternal vitamin D status with the risk of preeclampsia

Author

Juhi Nema, Nisha Wadhwani, Karuna Randhir, Kamini Dangat, Hemlata Pisal, Vrushali Kadam, Savita Mehendale, Girija Wagh, Bharati Kulkarni, Harshpal Singh Sachdev, Caroline Fall, Sanjay Gupte, and Sadhana Joshi

Subjects
General Medicine, Food Science
Abstract

The aim of this study was to examine serum vitamin D concentrations from early pregnancy until delivery in women who did and did not develop preeclampsia.

Published
2023

17. Endoscopic Submucosal Dissection for Esophageal Squamous Cell High-grade Dysplasia in a Patient with Plummer Vinson Syndrome

Author

Gaurav Patil, Amol Vadgaonkar, Ankit Dalal, Sanil Parekh, Animesh Shah, Poorva Haridas, Prajakta Gupte, Sehajad Vora, and Amit Maydeo

Subjects
General Environmental Science
Abstract

A 35-year female with Plummer Vinson syndrome (PVS) presented with a history of progressive dysphagia over six months, not responding to iron therapy and endoscopic dilatations. Her upper gastrointestinal endoscopy showed a post-cricoid web dilated using a Savary-Gilliard dilator. On NBI, a long segment circumferential lesion with abnormal microvascular architecture was noted in the mid esophagus. Biopsy showed high-grade dysplastic squamous epithelium. The patient underwent minimally invasive, circumferential endoscopic submucosal dissection (ESD) and received oral prednisolone to prevent stricture formation. Resected margins were free of dysplasia. At follow-up there was no evidence of recurrence or stricture formation. To our knowledge, this is the first case of PVS with squamous proliferation with high-grade dysplasia that was successfully treated with circumferential ESD. Screening endoscopy helps in the downstaging of early cancer, and timely intervention helps to treat this with a minimally invasive approach like ESD.

Published
2022

19. Evaluation of Bioactive Attributes and Emulsification Potential of Exopolysaccharide Produced by a Brown-rot Fungus Fomitopsis meliae AGDP-2

Author

Darshankumar Prajapati, Ashish Bhatt, and Akshaya Gupte

Subjects
Bioengineering, General Medicine, Molecular Biology, Applied Microbiology and Biotechnology, Biochemistry, Biotechnology
Abstract

Mushrooms possess wide array of biologically active secondary metabolites and have been traditionally used for their medicinal properties. Exopolysaccharide (EPS) is one of such bioactive metabolites. The bioactive attributes and emulsification capabilities of the exopolysaccharides produced by a novel brown-rot fungus Fomitopsis meliae AGDP-2 under submerged fermentation has been thoroughly investigated in the present study. Exopolysaccharide displayed anti-oxidant activities in dose dependent manner with the maximum scavenging of ABTS radicals (42.45%), DPPH radicals (75.34%), Hydroxyl radicals (63.64%), Superoxide anion radical (76.54%) and Ferric Reducing Antioxidant Power with IC

Published
2022

21. Gray squirrels consume anthropogenic food waste most often during winter

Author

Rebecca Rimbach, Gabrielle Butler, Pratik R. Gupte, Jörg Jäger, Claire Parker, Herman Pontzer, and Weissing group

Subjects
Waste, Urbanization, Trash bin, Anthropogenic food sources, Population density, Animal Science and Zoology, Garbage, Ecology, Evolution, Behavior and Systematics
Abstract

Urban habitats provide wildlife with predictable, easily accessible and abundant food sources in the form of human food waste. Urban eastern gray squirrels (Sciurus carolinensis) are commonly observed feeding in trash bins, but we lack data regarding the type, quantity and seasonal changes in food waste usage. We observed five trash bins on an urban university campus during four different observation periods. We recorded the time squirrels spent on and inside trash bins and type of retrieved food items. We also recorded ambient temperature, human presence and trash bin filling. Moreover, we determined changes in squirrel population density in a natural and three anthropogenic habitats during the same periods. Trash bins were fuller when human presence was higher. The higher human presence, the more squirrels went on and inside the bin, but there was no effect on number of retrieved food items. Trash bin usage by squirrels decreased when ambient temperature and bin filling increased. Most food items were retrieved during the coldest observation period, a period of high human presence, and the majority of retrieved food items were starchy foods (e.g., bread, French fries). The relationship between the number of squirrels observed along transects and a measure of urbanization, the normalized difference built-up index, was negative in periods with high ambient temperatures and positive in periods with low ambient temperatures, indicating winter may be less challenging in urban areas, likely facilitated by the availability of anthropogenic food sources, allowing a higher level of activity throughout winter.

Published
2022

22. A scoping review of interventions to mitigate common non-communicable diseases among people with TB

Author

K, Romanowski, A, Oravec, M, Billingsley, K, Shearer, A, Gupte, M A, Huaman, G J, Fox, J E, Golub, and J C, Johnston

Subjects
Counseling, Pulmonary and Respiratory Medicine, Infectious Diseases, Alcohol Drinking, Substance-Related Disorders, Humans, Comorbidity, Noncommunicable Diseases
Abstract

BACKGROUND: Recommendations have been made to integrate screening for common non-communicable diseases (NCDs) within TB programs. However, we must ensure screening is tied to evidence-based interventions before scale-up. We aimed to map the existing evidence regarding interventions that address NCDs that most commonly affect people with TB.METHODS: We systematically searched PubMed, Medline, and Embase for studies that evaluated interventions to mitigate respiratory disease, cardiovascular disease, alcohol and substance use disorder, and mental health disorders among people with TB. We excluded studies that only screened for comorbidity but resulted in no further intervention. We also excluded studies focusing on smoking cessation interventions for which evidence-based guidelines are well established.RESULTS: The search identified 20 studies that met our inclusion criteria. The most commonly evaluated intervention was referral for diabetes care (6 studies). Other interventions included pulmonary rehabilitation (5 studies), care programs for alcohol use disorder (4 studies), and psychosocial support or individual counselling (5 studies).CONCLUSION: There is limited robust evidence to support identified interventions in changing individual outcomes, and a significant knowledge gap remains on the long-term durability of the interventions´ clinical benefit, reach, and effectiveness. Implementation research demonstrating feasibility and effectiveness is needed before scaling up.

Published
2022

23. Concomitant pulmonary disease is common among patients with extrapulmonary TB

Author

S V B Y, Shivakumar, C, Padmapriyadarsini, A, Chavan, M, Paradkar, B M, Shrinivasa, A, Gupte, K, Dhanasekaran, B, Thomas, N, Suryavanshi, C K, Dolla, S, Selvaraju, A, Kinikar, S, Gaikwad, R, Kohli, G N, Sivaramakrishnan, N, Pradhan, L E, Hanna, V, Kulkarni, A, DeLuca, S R, Cox, L, Murali, K, Thiruvengadam, S, Raskar, G, Ramachandran, J E, Golub, N, Gupte, V, Mave, S, Swaminathan, A, Gupta, and R C, Bollinger

Subjects
Adult, Pulmonary and Respiratory Medicine, Infectious Diseases, Cough, Sputum, Humans, India, Tuberculosis, Child, Tuberculosis, Pulmonary
Abstract

BACKGROUND: Microbiologic screening of extrapulmonary TB (EPTB) patients could inform recommendations for aerosol precautions and close contact prophylaxis. However, this is currently not routinely recommended in India. Therefore, we estimated the proportion of Indian patients with EPTB with microbiologic evidence of pulmonary TB (PTB).METHODS: We characterized baseline clinical, radiological and sputum microbiologic data of 885 adult and pediatric TB patients in Chennai and Pune, India, between March 2014 and November 2018.RESULTS: Of 277 patients with EPTB, enhanced screening led to the identification of 124 (45%) with concomitant PTB, including 53 (19%) who reported a cough >2 weeks; 158 (63%) had an abnormal CXR and 51 (19%) had a positive sputum for TB. Of 70 participants with a normal CXR and without any cough, 14 (20%) had a positive sputum for TB. Overall, the incremental yield of enhanced screening of patients with EPTB to identify concomitant PTB disease was 14% (95% CI 12–16).CONCLUSIONS: A high proportion of patients classified as EPTB in India have concomitant PTB. Our results support the need for improved symptom and CXR screening, and recommends routine sputum TB microbiology screening of all Indian patients with EPTB.

Published
2022

24. Previous tuberculosis disease as a risk factor for chronic obstructive pulmonary disease

Author

Robert A. Wise, Antonio Bernabe-Ortiz, Suzanne L. Pollard, Akshay Gupte, Frederik van Gemert, Dewan S. Alam, Laura Gutierrez, J. Jaime Miranda, Muhammad Ashique Haider Chowdhury, William Checkley, Trishul Siddharthan, Shakir Hossen, Rupert Jones, Katarina Kamenar, Vilma Irazola, Bruce Kirenga, and Adolfo Rubinstein

Subjects
Pulmonary and Respiratory Medicine, Male, medicine.medical_specialty, Vital capacity, Tuberculosis, Population, Vital Capacity, URBAN, AIR-FLOW OBSTRUCTION, FEV1/FVC ratio, Pulmonary Disease, Chronic Obstructive, Risk Factors, Internal medicine, Forced Expiratory Volume, medicine, Humans, COPD, Risk factor, education, Tuberculosis, Pulmonary, History of tuberculosis, education.field_of_study, business.industry, ASSOCIATION, Middle Aged, medicine.disease, respiratory tract diseases, PREVALENCE, FUNCTION IMPAIRMENT, Cross-Sectional Studies, tuberculosis, Spirometry, Attributable risk, Female, business, COPD epidemiology, CHRONIC RESPIRATORY-DISEASE
Abstract

BackgroundRisk factors for COPD in high-income settings are well understood; however, less attention has been paid to contributors of COPD in low-income and middle-income countries (LMICs) such as pulmonary tuberculosis. We sought to study the association between previous tuberculosis disease and COPD by using pooled population-based cross-sectional data in 13 geographically diverse, low-resource settings.MethodsWe pooled six cohorts in 13 different LMIC settings, 6 countries and 3 continents to study the relationship between self-reported previous tuberculosis disease and lung function outcomes including COPD (defined as a postbronchodilator forced expiratory volume in one second (FEV1)/forced vital capacity (FVC) below the lower limit of normal). Multivariable regressions with random effects were used to examine the association between previous tuberculosis disease and lung function outcomes.ResultsWe analysed data for 12 396 participants (median age 54.0 years, 51.5% male); 332 (2.7%) of the participants had previous tuberculosis disease. Overall prevalence of COPD was 8.8% (range 1.7%–15.5% across sites). COPD was four times more common among those with previous tuberculosis disease (25.7% vs 8.3% without previous tuberculosis disease, p1(−0.70, 95% CI −0.84 to −0.55), FVC (−0.44, 95% CI −0.59 to −0.29) and the FEV1:FVC ratio (−0.63, 95% CI −0.76 to −0.51) when compared with those without previous tuberculosis disease.ConclusionsPrevious tuberculosis disease is a significant and under-recognised risk factor for COPD and poor lung function in LMICs. Better tuberculosis control will also likely reduce the global burden of COPD.

Published
2022

25. Longitudinal Assessment of Calcium and Magnesium Levels in Women with Preeclampsia

Author

Nisha, Wadhwani, Kamini, Dangat, Karuna, Randhir, Anupam, Poddar, Prachi, Joshi, Hemlata, Pisal, Vrushali, Kadam, Ravleen, Bakshi, Nomita, Chandhiok, Sanjay, Lalwani, Savita, Mehendale, Girija, Wagh, Sanjay, Gupte, Harshpal Singh, Sachdev, Caroline, Fall, and Sadhana, Joshi

Subjects
Inorganic Chemistry, Endocrinology, Diabetes and Metabolism, Biochemistry (medical), Clinical Biochemistry, General Medicine, Biochemistry
Abstract

The present study reports the levels of maternal serum calcium and magnesium from early pregnancy until delivery, along with cord levels, in women who developed preeclampsia (PE) and compares them with those without PE. A total of 324 pregnant women (216 non-PE and 108 PE women) were included in this retrospective case-control study of prospectively collected data nested in an observational cohort study. Maternal blood was collected at 4 time points during pregnancy (V1 = 11-14 weeks, V2 = 18-22 weeks, V3 = 26-28 weeks, and V4 = at delivery) and umbilical cord blood at delivery. Independent t tests were used to compare calcium, magnesium, and their ratio between two groups, and their associations with PE were studied using regression models. Calcium levels were similar between groups at all time points. Magnesium levels were lower (p = 0.021) at V2 in PE group as compared with non-PE group. Maternal calcium and magnesium levels were negatively associated, with blood pressure in early pregnancy. In fully adjusted logistic regression analysis, lower magnesium levels were associated with an increased risk of PE at V2 (OR 0.25 [95% CI 0.07, 0.94] p = 0.04). Lower magnesium in mid-pregnancy was associated with higher risk of PE. These changes were observed before the diagnosis of PE, thereby suggesting that they may have a role in the etiology of PE.

Published
2022

26. Acute Hepatitis of Unknown Etiology Among Young Children: Research Agenda by the ESPGHAN Hepatology Committee

Author

Giuseppe, Indolfi, Piotr, Czubkowski, Emer, Fitzpatrick, Emmanuel, Gonzales, Girish, Gupte, Sara, Mancell, Yael, Mozer-Glassberg, Emanuele, Nicastro, Junge, Norman, Xavier, Stephenne, Aglaia, Zellos, Marianne, Samyn, UCL - SSS/IREC/PEDI - Pôle de Pédiatrie, and UCL - (SLuc) Service de gastro-entérologie et hépatologie pédiatrique

Subjects
COVID-19 Vaccines, SARS-CoV-2, Child, Preschool, Acute Disease, Pediatrics, Perinatology and Child Health, Gastroenterology, COVID-19, Humans, Perinatology and Child Health, Child, Pediatrics, Societies, Medical, Hepatitis
Abstract

In April 2022, an increased incidence of acute hepatitis cases of unknown etiology among previously healthy children across the United Kingdom was described. Since, more than 270 cases from the United Kingdom and hundreds more from all across the world have been reported. The majority of affected children were younger than 6 years of age. The clinical presentation was nonspecific with diarrhea and vomiting usually preceding the appearance of jaundice, abdominal pain, nausea, and malaise. Approximately 5% have required liver transplantation. An infectious etiology has been considered likely given the epidemiological and clinical features of the reported cases. Between 50 and 60% of the children tested were diagnosed with adenovirus infection although a clear etiological connection has still to be demonstrated. No link with SARS-CoV-2 infection and COVID-19 vaccine was found. What is not clear to date is whether the high number of acute hepatitis cases reported is related to a true increase in incidence or heightened awareness following on from the initial reports from the United Kingdom. The Hepatology Committee of the European Society of Pediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) developed a paper on the current outbreak of acute hepatitis of unknown etiology recognizing its importance and the need of approaching the current situation with a scientifically rigorous approach. The aims of the article are to summarize the current knowledge and to identify the most pertinent issues regarding the diagnosis and management of this condition and the research questions raised.

Published
2022

27. Urban Popular Economies

Author

Benjamin, Solomon, Castronovo, Alioscia, Cavallero, Luci, Cielo, Cristina, Gago, Véronica, Guma, Prince, Gupte, Rupali, Habermehl, Victoria, Salman, Lana, Shetty, Prasad, Simone, AbdouMaliq, Smith, Constance, and Tonucci, João

Subjects
Arts and Humanities (miscellaneous), Social Psychology, Communication
Abstract

What is a life worth living and how is it concretely actualized by an urban majority making often unanticipated, unformatted uses of the urban to engender livelihoods in a dynamic and open-ended process? This is the key question undertaken in this collectively written piece. This means thinking about work, paid and unpaid, in ways that highlight the everyday practices of urban inhabitants as they put together territories in which to operate, which sustain their imaginations of well-being as part of a process of being with others—in households, neighborhoods, communities, and institutions. What is it that different kinds of workers have in common; what links them; where does the household begin and end; what is the difference between productive and reproductive work?

Published
2022

28. Odevixibat treatment in progressive familial intrahepatic cholestasis

Author

Richard J Thompson, Henrik Arnell, Reha Artan, Ulrich Baumann, Pier Luigi Calvo, Piotr Czubkowski, Buket Dalgic, Lorenzo D'Antiga, Özlem Durmaz, Björn Fischler, Emmanuel Gonzalès, Tassos Grammatikopoulos, Girish Gupte, Winita Hardikar, Roderick H J Houwen, Binita M Kamath, Saul J Karpen, Lise Kjems, Florence Lacaille, Alain Lachaux, Elke Lainka, Cara L Mack, Jan P Mattsson, Patrick McKiernan, Hasan Özen, Sanjay R Rajwal, Bertrand Roquelaure, Mohammad Shagrani, Eyal Shteyer, Nisreen Soufi, Ekkehard Sturm, Mary Elizabeth Tessier, Henkjan J Verkade, Patrick Horn, and Center for Liver, Digestive and Metabolic Diseases (CLDM)

Subjects
Cholestasis, Adolescent, Hepatology, Pruritus, Medizin, Gastroenterology, Infant, Cholestasis, Intrahepatic, Bile Acids and Salts, Benzodiazepines, Butyrates, Child, Preschool, Humans, Child
Abstract

BACKGROUND: Progressive familial intrahepatic cholestasis (PFIC) is a group of inherited paediatric liver diseases resulting from mutations in genes that impact bile secretion. We aimed to evaluate the effects of odevixibat, an ileal bile acid transporter inhibitor, versus placebo in children with PFIC.METHODS: Patients eligible for this 24-week, randomised, double-blind, completed, phase 3 study were paediatric outpatients diagnosed with PFIC1 or PFIC2 who had pruritus and elevated serum bile acids at screening. Patients were randomly assigned (1:1:1) using an interactive web-based system to once a day oral placebo, odevixibat 40 μg/kg, or odevixibat 120 μg/kg. Randomisation was done in a block size of six and stratified by PFIC type and patient age; patients, clinicians, and study staff were blinded to treatment allocation. Patients were enrolled at one of 33 global sites. Two primary endpoints were evaluated: proportion of positive pruritus assessments (PPAs; ie, scratching score of ≤1 or ≥1-point decrease as assessed by caregivers using the Albireo observer-reported outcome [ObsRO] PRUCISION instrument) over 24 weeks, and proportion of patients with serum bile acid response (ie, serum bile acids reduced by ≥70% from baseline or concentrations of ≤70 μmol/L) at week 24. Efficacy and safety were analysed in randomly allocated patients who received one or more doses of study drug. This study is registered with ClinicalTrials.gov, NCT03566238.FINDINGS: Between June 21, 2018, and Feb 10, 2020, 62 patients (median age 3·2 [range 0·5-15·9] years) were randomly allocated to placebo (n=20), odevixibat 40 μg/kg per day (n=23), or odevixibat 120 μg/kg per day (n=19). Model-adjusted (least squares) mean proportion of PPAs was significantly higher with odevixibat versus placebo (55% [SE 8] in the combined odevixibat group [58% in the 40 μg/kg per day group and 52% in the 120 μg/kg per day group] vs 30% [SE 9] in the placebo group; model-adjusted mean difference 25·0% [95% CI 8·5-41·5]; p=0·0038). The percentage of patients with serum bile acid response was also significantly higher with odevixibat versus placebo (14 [33%] of 42 patients in the combined odevixibat group [10 in the 40 μg/kg per day group and four in the 120 μg/kg per day group] vs none of 20 in the placebo group; adjusting for stratification factor [PFIC type], the proportion difference was 30·7% [95% CI 12·6-48·8; p=0·0030]). The most common treatment-emergent adverse events (TEAEs) were diarrhoea or frequent bowel movements (13 [31%] of 42 for odevixibat vs two [10%] of 20 for placebo) and fever (12 [29%] of 42 vs five [25%] of 20); serious TEAEs occurred in three (7%) of 42 odevixibat-treated patients and in five (25%) of 20 placebo-treated patients.INTERPRETATION: In children with PFIC, odevixibat effectively reduced pruritus and serum bile acids versus placebo and was generally well tolerated. Odevixibat, administered as once a day oral capsules, is a non-surgical, pharmacological option to interrupt the enterohepatic circulation in patients with PFIC.FUNDING: Albireo Pharma.

Published
2022

29. small forces

Author

Rupali Gupte and Prasad Shetty

Subjects
Arts and Humanities (miscellaneous), Social Psychology, Communication
Abstract

This essay discusses cities as composites of small forces of energetic selves. Energetic self here is the dimension of the self that drives one to undertake activities connected to one's desires. These could include collecting strange objects, achieving mundane targets, opposing new ideas, behaving like a spy, counting every tree, tracking obscure data, occupying obscure spaces, and so on. Energetic selves also express themselves in everyday friendships and compassions. These practices go beyond the acts of routine and are considered unproductive in conceptualizing cities. They remain small and are often discarded as stray individual preoccupations, anecdotes, or subjective obsessions. While some of these are related to earnings and occupations, others are simply “useless.” However, everyone seems to have a trip that one lives with and for, and which provides individuals with their energies and cities with their oneiric spaces. Such energies, expressed in absurd quests, unusual obsessions, and bizarre interests cumulatively appear to be producing the city. In many ways the city seems to be a madhouse and madness seems to be running it. The city seems to acquire its generative energy from such small forces. Urban theory and pedagogy have however seldom engaged with an understanding of these small forces or extended it for speculative or projective purposes. Spatial professionals often take up the burden of acting like and being the modern state, which has to operate through modern imperatives of empiricism, technolegality, property regimes, boundaries, and so on. But while these imperatives are limited in understanding life, they are also not completely capable of handling the complexities of the urban. The paper further discusses a variety of ideas like settling; semi-fictional stories and montages; the blur; and transactional capacities to rethink the ways in which one could articulate newer ways to engage with the city.

Published
2022

30. Pineal Parenchymal Tumor of Intermediate Differentiation and DICER1 Syndrome: A Case Report

Author

Avanti, Gupte, Sandeep, Sood, William J, Kupsky, Deniz, Altinok, Steven, Miller, Sumita, Roy, and Kanta, Bhambhani

Subjects
Oncology, Pediatrics, Perinatology and Child Health, Hematology
Abstract

DICER1 syndrome is a rare inherited tumor predisposition syndrome associated with an increased risk for several malignant and benign tumors. We present a patient with pineal parenchymal tumor of intermediate differentiation who was found to have a germline pathogenic variant in DICER1 gene. Pineoblastoma is a known DICER1-related tumor; however, the association between pineal parenchymal tumor of intermediate differentiation and DICER1 mutation is rare with only 1 recent large molecular study that has reported this association. This report adds to the evolving tumor spectrum of DICER1 and highlights the importance of molecular evaluation of pediatric brain tumors, for both therapeutic decisions and long-term surveillance.

Published
2022

32. Statistical Modeling and Analysis of Online Examinations in Covid-19

Author

R. Gupte and R. Ramakrishnan

Abstract

In this paper, the authors build a model that predicts the grade point from a collection of independent variables including student characteristics and exam marks achieved in four marketing management courses using data from four courses. The data from four courses in marketing management, for an off-line class in a master’s in business management program taught in 2019 and the same set of four courses in marketing management taught to an on-line class in 2020 was taken. Although the number of students enrolled was almost equal, and the courses, despite being offered a year apart, were nearly comparable in structure and content, the teaching and assessment for 2019 was conducted offline, whereas it was conducted online in 2020. In the set of four courses offered in 2019 using the offline mode, the exam was proctored and offline but for the same courses offered in 2020, the final exam was also proctored but online. The authors predicted that if exams were taken without any misconduct, the prediction model would have the same explanatory power for all exams, and that if there was malpractice, the explanatory power would be lower. Their findings show that the two datasets are similar; there are variations in the independent variables that statistically and significantly predicted the Grade Point Average (GPA). The R-squared statistic suggests that the model for prediction is strong. Hence there is reason to believe that malpractice was taking place when the examinations were online, in spite of it being proctored. The goal of this paper is to provide teachers with practical ideas for administering proctored tests in their online courses.

Published
2022

33. Clinical Spectrum of Children with Acute Hepatitis of Unknown Cause

Author

Chayarani Kelgeri, Michael Couper, Girish L. Gupte, Alexandra Brant, Mitul Patel, Lauren Johansen, Joseph Valamparampil, Evelyn Ong, Hermien Hartog, M.T.P.R. Perera, Darius Mirza, Indra van Mourik, Khalid Sharif, and Jane Hartley

Subjects
General Medicine
Published
2022

34. Bicondylar Tibial Plateau Fractures: What Predicts Infection?

Author

Nathan Olszewski, Givenchy Manzano, Eleanor Wilson, Noah Joseph, Heather Vallier, Amanda Pawlak, Stephen Kottmeier, Adam Miller, Joshua Gary, Joshua Namm, Anna Miller, Guarang Gupte, Andre Rodriguez-Buitrago, William Obremskey, Donald Willier, Andrew Marcantonio, Laura Phieffer, Elizabeth Sheridan, Katerine Li, Madhav Karunakar, Juan Vargas-Hernandez, Brandon Yuan, Joshua Shapiro, Lincoln Pratson, Darin Friess, David Jenkins, Ross Leighton, Rashed Alqudhaya, Waael Aljilani, Brian Mullis, Konstantin Gruenwald, Benjamin Ollivere, Yulanda Myint, Christopher Odom, Clay Spitler, Patrik Suwak, Sagar Shah, Daniela Rocha, Daniel Horwitz, and Paul Tornetta

Subjects
Tibial Fractures, Fracture Fixation, Internal, Open Fracture Reduction, Treatment Outcome, Fracture Fixation, Humans, Orthopedics and Sports Medicine, Surgery, Retrospective Studies
Abstract

The purpose of this study was to identify the patient, injury, and treatment factors associated with infection of bicondylar plateau fractures and to evaluate whether center variation exists.Retrospective review.Eighteen academic trauma centers.A total of 1,287 patients with 1,297 OTA type 41-C bicondylar tibia plateau fractures who underwent open reduction and internal fixation were included. Exclusion criteria were follow-up less than 120 days, insufficient documentation, and definitive treatment only with external fixation.Open reduction and internal fixation.Superficial and deep infection.One hundred one patients (7.8%) developed an infection. In multivariate regression analysis, diabetes (DM) (OR [odds ratio] 3.24; P ≤ 0.001), alcohol abuse (EtOH) (OR 1.8; P = 0.040), dual plating (OR 1.8; P ≤ 0.001), and temporary external fixation (OR 2.07; P = 0.013) were associated with infection. In a risk-adjusted model, we found center variation in infection rates (P = 0.030).In a large series of patients undergoing open reduction and internal fixation of bicondylar plateau fractures, the infection rate was 7.8%. Infection was associated with DM, EtOH, combined dual plating, and temporary external fixation. Center expertise may also play a role because one center had a statistically lower rate and two trended toward higher rates after adjusting for confounders.Level IV-Therapeutic retrospective cohort study.

Published
2022

35. Reverse Knowledge Distillation: Training a Large Model using a Small One for Retinal Image Matching on Limited Data

Author

Nasser, Sahar Almahfouz, Gupte, Nihar, and Sethi, Amit

Subjects
FOS: Computer and information sciences, Computer Vision and Pattern Recognition (cs.CV), Computer Science - Computer Vision and Pattern Recognition
Abstract

Retinal image matching plays a crucial role in monitoring disease progression and treatment response. However, datasets with matched keypoints between temporally separated pairs of images are not available in abundance to train transformer-based model. We propose a novel approach based on reverse knowledge distillation to train large models with limited data while preventing overfitting. Firstly, we propose architectural modifications to a CNN-based semi-supervised method called SuperRetina that help us improve its results on a publicly available dataset. Then, we train a computationally heavier model based on a vision transformer encoder using the lighter CNN-based model, which is counter-intuitive in the field knowledge-distillation research where training lighter models based on heavier ones is the norm. Surprisingly, such reverse knowledge distillation improves generalization even further. Our experiments suggest that high-dimensional fitting in representation space may prevent overfitting unlike training directly to match the final output. We also provide a public dataset with annotations for retinal image keypoint detection and matching to help the research community develop algorithms for retinal image applications.

Published
2023

37. Insight into the increased risk associated with CRISPR-generated African G6PD variant (N126D) in rat model of sugen-5416-induced pulmonary hypertension

Author

Christina Signoretti, Shun Matsumura, Samuel Fatehi, and Sachin Gupte

Subjects
Physiology
Abstract

Pulmonary Hypertension (PH) is a cardiopulmonary disease associated with sustained increase in pulmonary arterial and right ventricular pressures, resulting in smooth muscle cell remodeling and the thickening of vasculature. Glucose-6-phosphate dehydrogenase (G6PD) is the rate limiting enzyme in the pentose phosphate pathway and is often associated with oxidative stress and the pulmonary vascular remodeling mechanism seen in PH. CRISPR-mediated single nucleotide polymorphism modeling was used to generate the African G6PD variant (N126D; G6PDN126D), which is an X-linked gene mutation resulting in a 20% decrease in G6PD activity and subsequent hypothesized increased risk of cardiovascular diseases for sub-Saharan individuals. The goal of this study was to determine the effects of the G6PDN126D variant using the Sugen-5416 (20 mg/kg in DMSO)/Normoxia (SU/NX)-induced PH rat model. Cardiac catheterization determined that the G6PDN126D variant-SU/Nx group had higher (G6PDN126D: 29.3 ± 5.2 vs WT: 19.5 ± 2.2; mmHg; pN126D variant-SU/Nx had lower nitric oxide levels (G6PDN126D: 10.6 vs WT: 13.6; mM/mg protein; pN126D: 4.00 vs WT: 0.52; ng/mg protein; p RO1HL132574 (SAG), RO1HL166546 (SAG), AHA Grant-in-Aid 17GRNT33670454 (SAG) This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Published
2023

38. Controversy and consensus on the management of elevated sperm DNA fragmentation in male infertility: a global survey, current guidelines, and expert recommendations

Author

Ala'a Farkouh, Ashok Agarwal, Taha Abo-Almagd Abdel-Meguid Hamoda, Parviz Kavoussi, Ramadan Saleh, Armand Zini, Mohamed Arafa, Ahmed M Harraz, Murat Gul, Vilvapathy Senguttuvan Karthikeyan, Damayanthi Durairajanayagam, Amarnath Rambhatla, Florence Boitrelle, Eric Chung, Ponco Birowo, Tuncay Toprak, Ramy Abou Ghayda, Rossella Cannarella, Nguyen Ho Vinh Phuoc, Fotios Dimitriadis, Giorgio I Russo, Ioannis Sokolakis, Taymour Mostafa, Konstantinos Makarounis, Imad Ziouziou, Shinnosuke Kuroda, Marion Bendayan, Raneen Sawaid Kaiyal, Andrian Japari 33, Mara Simopoulou 34, Lucia Rocco, Nicolas Garrido 36, Nazim Gherabi 37, Kadir Bocu 38, Oguzhan Kahraman 39, Tan V Le 27 28, Christine Wyns 40, Kelton Tremellen 41, Selcuk Sarikaya 42, Sheena Lewis 43, Donald P Evenson 44, Edmund Ko 45, Aldo E Calogero 25, Fahmi Bahar 46, Marlon Martinez 47, Andrea Crafa 25, Quang Nguyen 48 49, Rafael F Ambar 50 51, Giovanni Colpi 52, Mustafa Emre Bakircioglu 53, Ralf Henkel 54 55, Hussein Kandil 56, Ege Can Serefoglu 57, Abdullah Alarbid 13, Akira Tsujimura 58, Alireza Kheradmand 59, Christina Anagnostopoulou 60, Angelo Marino 61, Aram Adamyan 62, Birute Zilaitiene 63, Cevahir Ozer 39, Edoardo Pescatori 64, Paraskevi Vogiatzi 65, Gian Maria Busetto 66, Giancarlo Balercia 67, Haitham Elbardisi 9 10, Hamed Akhavizadegan 68, Hesamoddin Sajadi 69, Hisanori Taniguchi 70, Hyun Jun Park 71 72, Israel Maldonado Rosas 73, Mohamed Al-Marhoon 74, Mohammad Ali Sadighi Gilani 69, Naif Alhathal 75, Germar-Michael Pinggera 76, Priyank Kothari 77, Nasser Mogharabian 78, Sava Micic 79, Sheryl Homa 80, Sara Darbandi 81 82, Tran Quang Tien Long 83, Wael Zohdy 10, Widi Atmoko 22, Marjan Sabbaghian 69, Wael Ibrahim 84, Ryan P Smith 85, Christopher Chee Kong Ho 86, Jean de la Rosette 87, Ahmed I El-Sakka 88, Mirko Preto 89, Cătălina Zenoaga-Barbăroșie 90, Saad Mohammed Abumelha 91, Aykut Baser 92, Kaan Aydos 93, Liliana Ramirez-Dominguez 73, Vijay Kumar 94, Teng Aik Ong 95, Tiago Cesar Mierzwa 96, Ricky Adriansjah 97, Saleem A Banihani 98, Kasonde Bowa 99, Shinichiro Fukuhara 100, Marcelo Rodriguez Peña 101, Mohamad Moussa 102 103, Umut Çağın Ari 104, Chak-Lam Cho 105, Nicholas N Tadros 106, Muhammet Rasit Ugur 107, Edouard Amar 108, Marco Falcone 109, Frederic Romain Santer 76, Arif Kalkanli 110, Keshab Kumar Karna 111, Kareim Khalafalla 9 112 113, Ranjit B Vishwakarma 114, Federica Finocchi 67, Carlo Giulioni 115, Erman Ceyhan 39, Gökhan Çeker 116, Chadi Yazbeck 117, Osvaldo Rajmil 118, Mehmet Yilmaz 119, Baris Altay 120, Trenton L Barrett 121, Kay Seong Ngoo 122, Shubhadeep Roychoudhury 123, Gianmaria Salvio 67, Haocheng Lin 124, Ates Kadioglu 125, Massimiliano Timpano 109, Tomer Avidor-Reiss 126 127, Lukman Hakim 128, Puneet Sindhwani 128, Giorgio Franco 129, Rajender Singh 130, Filippo Giacone 131, Mikhail Ruzaev 132, Raghavender Kosgi 133, Nikolaos Sofikitis 134, Ayad Palani 135, Gokhan Calik 87, Deniz Kulaksız 136, Davor Jezek 137, Manaf Al Hashmi 138 139, Panagiotis Drakopoulos 140 141, Huda Omran 142, Sofia Leonardi 143, Ciler Celik-Ozenci 144, Nur Dokuzeylül Güngör 145, Jonathan Ramsay 146, Toshiyasu Amano 147, Emrullah Sogutdelen 148, Gede Wirya Kusuma Duarsa 149, Koji Chiba 150, Sunil Jindal 151, Missy Savira 22, Luca Boeri 152, Edson Borges 153, Deepak Gupte 154, Fatih Gokalp 155, Guadalupe Hernández Hebrard 73, Suks Minhas 156, Rupin Shah 114, Global Andrology Forum, Farkouh, Ala'A, Agarwal, Ashok, Abo-Almagd Abdel-Meguid Hamoda, Taha, Kavoussi, Parviz, Saleh, Ramadan, Zini, Armand, Arafa, Mohamed, M Harraz, Ahmed, Gul, Murat, Senguttuvan Karthikeyan, Vilvapathy, Durairajanayagam, Damayanthi, Rambhatla, Amarnath, Boitrelle, Florence, Chung, Eric, Birowo, Ponco, Toprak, Tuncay, Abou Ghayda, Ramy, Cannarella, Rossella, Ho Vinh Phuoc, Nguyen, Dimitriadis, Fotio, I Russo, Giorgio, Sokolakis, Ioanni, Mostafa, Taymour, Makarounis, Konstantino, Ziouziou, Imad, Kuroda, Shinnosuke, Bendayan, Marion, Sawaid Kaiyal, Raneen, Japari 33, Andrian, Simopoulou 34, Mara, Rocco, Lucia, Garrido 36, Nicola, Gherabi 37, Nazim, Bocu 38, Kadir, Kahraman 39, Oguzhan, V Le 27 28, Tan, Wyns 40, Christine, Tremellen 41, Kelton, Sarikaya 42, Selcuk, Lewis 43, Sheena, P Evenson 44, Donald, Ko 45, Edmund, E Calogero 25, Aldo, Bahar 46, Fahmi, Martinez 47, Marlon, Crafa 25, Andrea, Nguyen 48 49, Quang, F Ambar 50 51, Rafael, Colpi 52, Giovanni, Emre Bakircioglu 53, Mustafa, Henkel 54 55, Ralf, Kandil 56, Hussein, Can Serefoglu 57, Ege, Alarbid 13, Abdullah, Tsujimura 58, Akira, Kheradmand 59, Alireza, Anagnostopoulou 60, Christina, Marino 61, Angelo, Adamyan 62, Aram, Zilaitiene 63, Birute, Ozer 39, Cevahir, Pescatori 64, Edoardo, Vogiatzi 65, Paraskevi, Maria Busetto 66, Gian, Balercia 67, Giancarlo, 9 10, Haitham Elbardisi, Akhavizadegan 68, Hamed, Sajadi 69, Hesamoddin, Taniguchi 70, Hisanori, Jun Park 71 72, Hyun, Maldonado Rosas 73, Israel, Al-Marhoon 74, Mohamed, Ali Sadighi Gilani 69, Mohammad, Alhathal 75, Naif, Pinggera 76, Germar-Michael, Kothari 77, Priyank, Mogharabian 78, Nasser, Micic 79, Sava, Homa 80, Sheryl, Darbandi 81 82, Sara, Quang Tien Long 83, Tran, Zohdy 10, Wael, Atmoko 22, Widi, Sabbaghian 69, Marjan, Ibrahim 84, Wael, P Smith 85, Ryan, Chee Kong Ho 86, Christopher, de la Rosette 87, Jean, I El-Sakka 88, Ahmed, Preto 89, Mirko, Zenoaga-Barbăroșie 90, Cătălina, Mohammed Abumelha 91, Saad, Baser 92, Aykut, Aydos 93, Kaan, Ramirez-Dominguez 73, Liliana, Kumar 94, Vijay, Aik Ong 95, Teng, Cesar Mierzwa 96, Tiago, Adriansjah 97, Ricky, A Banihani 98, Saleem, Bowa 99, Kasonde, Fukuhara 100, Shinichiro, Rodriguez Peña 101, Marcelo, Moussa 102 103, Mohamad, Çağın Ari 104, Umut, Cho 105, Chak-Lam, N Tadros 106, Nichola, Rasit Ugur 107, Muhammet, Amar 108, Edouard, Falcone 109, Marco, Romain Santer 76, Frederic, Kalkanli 110, Arif, Kumar Karna 111, Keshab, 9 112 113, Kareim Khalafalla, B Vishwakarma 114, Ranjit, Finocchi 67, Federica, Giulioni 115, Carlo, Ceyhan 39, Erman, Çeker 116, Gökhan, Yazbeck 117, Chadi, Rajmil 118, Osvaldo, Yilmaz 119, Mehmet, Altay 120, Bari, L Barrett 121, Trenton, Seong Ngoo 122, Kay, Roychoudhury 123, Shubhadeep, Salvio 67, Gianmaria, Lin 124, Haocheng, Kadioglu 125, Ate, Timpano 109, Massimiliano, Avidor-Reiss 126 127, Tomer, Hakim 128, Lukman, Sindhwani 128, Puneet, Franco 129, Giorgio, Singh 130, Rajender, Giacone 131, Filippo, Ruzaev 132, Mikhail, Kosgi 133, Raghavender, Sofikitis 134, Nikolao, Palani 135, Ayad, Calik 87, Gokhan, Kulaksız 136, Deniz, Jezek 137, Davor, Al Hashmi 138 139, Manaf, Drakopoulos 140 141, Panagioti, Omran 142, Huda, Leonardi 143, Sofia, Celik-Ozenci 144, Ciler, Dokuzeylül Güngör 145, Nur, Ramsay 146, Jonathan, Amano 147, Toshiyasu, Sogutdelen 148, Emrullah, Wirya Kusuma Duarsa 149, Gede, Chiba 150, Koji, Jindal 151, Sunil, Savira 22, Missy, Boeri 152, Luca, Borges 153, Edson, Gupte 154, Deepak, Gokalp 155, Fatih, Hernández Hebrard 73, Guadalupe, Minhas 156, Suk, Shah 114, Rupin, and Andrology Forum, Global

Abstract

PURPOSE: Sperm DNA fragmentation (SDF) has been associated with male infertility and poor outcomes of assisted reproductive technology (ART). The purpose of this study was to investigate global practices related to the management of elevated SDF in infertile men, summarize the relevant professional society recommendations, and provide expert recommendations for managing this condition. MATERIALS AND METHODS: An online global survey on clinical practices related to SDF was disseminated to reproductive clinicians, according to the CHERRIES checklist criteria. Management protocols for various conditions associated with SDF were captured and compared to the relevant recommendations in professional society guidelines and the appropriate available evidence. Expert recommendations and consensus on the management of infertile men with elevated SDF were then formulated and adapted using the Delphi method. RESULTS: A total of 436 experts from 55 different countries submitted responses. As an initial approach, 79.1% of reproductive experts recommend lifestyle modifications for infertile men with elevated SDF, and 76.9% prescribe empiric antioxidants. Regarding antioxidant duration, 39.3% recommend 4-6 months and 38.1% recommend 3 months. For men with unexplained or idiopathic infertility, and couples experiencing recurrent miscarriages associated with elevated SDF, most respondents refer to ART 6 months after failure of conservative and empiric medical management. Infertile men with clinical varicocele, normal conventional semen parameters, and elevated SDF are offered varicocele repair immediately after diagnosis by 31.4%, and after failure of antioxidants and conservative measures by 40.9%. Sperm selection techniques and testicular sperm extraction are also management options for couples undergoing ART. For most questions, heterogenous practices were demonstrated. CONCLUSIONS: This paper presents the results of a large global survey on the management of infertile men with elevated SDF and reveals a lack of consensus among clinicians. Furthermore, it demonstrates the scarcity of professional society guidelines in this regard and attempts to highlight the relevant evidence. Expert recommendations are proposed to help guide clinicians.

Published
2023

39. Addition of tabulated equation of state and neutrino leakage support to i llinois grmhd

Author

Leonardo R. Werneck, Zachariah B. Etienne, Ariadna Murguia-Berthier, Roland Haas, Federico Cipolletta, Scott C. Noble, Lorenzo Ennoggi, Federico G. Lopez Armengol, Bruno Giacomazzo, Thiago Assumpção, Joshua Faber, Tanmayee Gupte, Bernard J. Kelly, Julian H. Krolik, Werneck, L, Etienne, Z, Murguia-Berthier, A, Haas, R, Cipolletta, F, Noble, S, Ennoggi, L, Lopez Armengol, F, Giacomazzo, B, Assumpcao, T, Faber, J, Gupte, T, Kelly, B, and Krolik, J

Subjects
High Energy Astrophysical Phenomena (astro-ph.HE), FOS: Physical sciences, General Relativity and Quantum Cosmology (gr-qc), numerical relativity, general relativity, magnetohydrodynamics, Astrophysics - High Energy Astrophysical Phenomena, Astrophysics - Instrumentation and Methods for Astrophysics, Instrumentation and Methods for Astrophysics (astro-ph.IM), General Relativity and Quantum Cosmology
Abstract

We have added support for realistic, microphysical, finite-temperature equations of state (EOS) and neutrino physics via a leakage scheme to IllinoisGRMHD, an open-source GRMHD code for dynamical spacetimes in the Einstein Toolkit. These new features are provided by two new, NRPy+-based codes: NRPyEOS, which performs highly efficient EOS table lookups and interpolations, and NRPyLeakage, which implements a new, AMR-capable neutrino leakage scheme in the Einstein Toolkit. We have performed a series of strenuous validation tests that demonstrate the robustness of these new codes, particularly on the Cartesian AMR grids provided by Carpet. Furthermore, we show results from fully dynamical GRMHD simulations of single unmagnetized neutron stars, and magnetized binary neutron star mergers. This new version of IllinoisGRMHD, as well as NRPyEOS and NRPyLeakage, is pedagogically documented in Jupyter notebooks and fully open source. The codes will be proposed for inclusion in an upcoming version of the Einstein Toolkit., Comment: 20 pages, 9 figures. v2 matches PRD version

Published
2023

42. Controversy and Consensus on Indications for Sperm DNA Fragmentation Testing in Male Infertility: A Global Survey, Current Guidelines, and Expert Recommendations

Author

Agarwal, A, Farkouh, A, Saleh, R, Abdel-Meguid Hamoda, TA-A, Harraz, AM, Kavoussi, P, Arafa, M, Salvio, G, Rambhatla, A, Toprak, T, Gül, M, Phuoc, NHV, Boitrelle, F, Birowo, P, Ghayda, RA, Cannarella, R, Kuroda, S, Durairajanayagam, D, Zini, A, Wyns, C, Sarikaya, S, Tremellen, K, Mostafa, T, Sokolakis, I, Evenson, DP, Henkel, R, Zohdy, W, Chung, E, Ziouziou, I, Falcone, M, Russo, GI, Al-Hashimi, M, Calogero, AE, Ko, E, Colpi, G, Lewis, S, Serefoglu, EC, Bahar, F, Martinez, M, Nguyen, Q, Ambar, RF, Bakircioglu, ME, Kandil, H, Mogharabian, N, Sabbaghian, M, Taniguchi, H, Tsujimura, A, Sajadi, H, Ibrahim, W, Atmoko, W, Vogiatzi, P, Gunes, S, Gilani, MAS, Roychoudhury, S, Güngör, ND, Hakim, L, Adriansjah, R, Kothari, P, Jindal, S, Amar, E, Park, HJ, Long, TQT, Homa, S, Karthikeyan, VS, Zilaitiene, B, Rosas, IM, Marino, A, Pescatori, E, Ozer, C, Akhavizadegan, H, Garrido, N, Busetto, GM, Adamyan, A, Al-Marhoon, M, Elbardisi, H, Dolati, P, Darbandi, M, Darbandi, S, Balercia, G, Pinggera, G-M, Micic, S, Ho, CCK, Moussa, M, Preto, M, Zenoaga-Barbăroșie, C, Smith, RP, Kosgi, R, Rosette, JDL, El-Sakka, AI, Abumelha, SM, Mierzwa, TC, Ong, TA, Banihani, SA, Bowa, K, Fukuhara, S, Boeri, L, Danacıoğlu, YO, Gokalp, F, Selim, OM, Cho, C-L, Tadros, NN, Ugur, MR, Ozkent, MS, Chiu, P, Kalkanli, A, Khalafalla, K, Vishwakarma, RB, Finocchi, F, Andreadakis, S, Giulioni, C, Çeker, G, Ceyhan, E, Malhotra, V, Yilmaz, M, Timpano, M, Barrett, TL, Kim, SHK, Ahn, S-T, Giacone, F, Palani, A, Duarsa, GWK, Kadioglu, A, Gadda, F, Zylbersztejn, DS, Aydos, K, Kulaksız, D, Gupte, D, Calik, G, Karna, KK, Drakopoulos, P, Baser, A, Kumar, V, Molina, JMC, Rajmil, O, Ferreira, RH, Leonardi, S, Avoyan, A, Sogutdelen, E, Franco, G, Ramsay, J, Ramirez, L, Shah, R, Global Andrology Forum, Agarwal, Ashok, Farkouh, Ala'A, Saleh, Ramadan, Abdel-Meguid Hamoda, Taha Abo-Almagd, Harraz, Ahmed M, Kavoussi, Parviz, Arafa, Mohamed, Salvio, Gianmaria, Rambhatla, Amarnath, Toprak, Tuncay, Gül, Murat, Phuoc, Nguyen Ho Vinh, Boitrelle, Florence, Birowo, Ponco, Ghayda, Ramy Abou, Cannarella, Rossella, Kuroda, Shinnosuke, Durairajanayagam, Damayanthi, Zini, Armand, Wyns, Christine, Sarikaya, Selcuk, Tremellen, Kelton, Mostafa, Taymour, Sokolakis, Ioanni, Evenson, Donald P, Henkel, Ralf, Zohdy, Wael, Chung, Eric, Ziouziou, Imad, Falcone, Marco, Russo, Giorgio I, Al-Hashimi, Manaf, Calogero, Aldo E, Ko, Edmund, Colpi, Giovanni, Lewis, Sheena, Serefoglu, Ege Can, Bahar, Fahmi, Martinez, Marlon, Nguyen, Quang, Ambar, Rafael F, Bakircioglu, Mustafa Emre, Kandil, Hussein, Mogharabian, Nasser, Sabbaghian, Marjan, Taniguchi, Hisanori, Tsujimura, Akira, Sajadi, Hesamoddin, Ibrahim, Wael, Atmoko, Widi, Vogiatzi, Paraskevi, Gunes, Sezgin, Gilani, Mohammad Ali Sadighi, Roychoudhury, Shubhadeep, Güngör, Nur Dokuzeylül, Hakim, Lukman, Adriansjah, Ricky, Kothari, Priyank, Jindal, Sunil, Amar, Edouard, Park, Hyun Jun, Long, Tran Quang Tien, Homa, Sheryl, Karthikeyan, Vilvapathy Senguttuvan, Zilaitiene, Birute, Rosas, Israel Maldonado, Marino, Angelo, Pescatori, Edoardo, Ozer, Cevahir, Akhavizadegan, Hamed, Garrido, Nicola, Busetto, Gian Maria, Adamyan, Aram, Al-Marhoon, Mohamed, Elbardisi, Haitham, Dolati, Parisa, Darbandi, Mahsa, Darbandi, Sara, Balercia, Giancarlo, Pinggera, Germar-Michael, Micic, Sava, Ho, Christopher Chee Kong, Moussa, Mohamad, Preto, Mirko, Zenoaga-Barbăroșie, Cătălina, Smith, Ryan P, Kosgi, Raghavender, Rosette, Jean de la, El-Sakka, Ahmed I, Abumelha, Saad Mohammed, Mierzwa, Tiago Cesar, Ong, Teng Aik, Banihani, Saleem A, Bowa, Kasonde, Fukuhara, Shinichiro, Boeri, Luca, Danacıoğlu, Yavuz Onur, Gokalp, Fatih, Selim, Osama Mohamed, Cho, Chak-Lam, Tadros, Nicholas N, Ugur, Muhammet Rasit, Ozkent, Mehmet Serkan, Chiu, Peter, Kalkanli, Arif, Khalafalla, Kareim, Vishwakarma, Ranjit B, Finocchi, Federica, Andreadakis, Sotiri, Giulioni, Carlo, Çeker, Gökhan, Ceyhan, Erman, Malhotra, Vineet, Yilmaz, Mehmet, Timpano, Massimiliano, Barrett, Trenton L, Kim, Shannon Hee Kyung, Ahn, Sun-Tae, Giacone, Filippo, Palani, Ayad, Duarsa, Gede Wirya Kusuma, Kadioglu, Ate, Gadda, Franco, Zylbersztejn, Daniel Suslik, Aydos, Kaan, Kulaksız, Deniz, Gupte, Deepak, Calik, Gokhan, Karna, Keshab Kumar, Drakopoulos, Panagioti, Baser, Aykut, Kumar, Vijay, Molina, Juan Manuel Corral, Rajmil, Osvaldo, Ferreira, Raphael H, Leonardi, Sofia, Avoyan, Armen, Sogutdelen, Emrullah, Franco, Giorgio, Ramsay, Jonathan, Ramirez, Liliana, Shah, Rupin, and Rocco, Lucia

Subjects
Male infertility, Practice guideline, Delphi method, DNA fragmentation, Survey, Sperm
Abstract

PURPOSE: Sperm DNA fragmentation (SDF) testing was recently added to the sixth edition of the World Health Organization laboratory manual for the examination and processing of human semen. Many conditions and risk factors have been associated with elevated SDF; therefore, it is important to identify the population of infertile men who might benefit from this test. The purpose of this study was to investigate global practices related to indications for SDF testing, compare the relevant professional society guideline recommendations, and provide expert recommendations. MATERIALS AND METHODS: Clinicians managing male infertility were invited to take part in a global online survey on SDF clinical practices. This was conducted following the CHERRIES checklist criteria. The responses were compared to professional society guideline recommendations related to SDF and the appropriate available evidence. Expert recommendations on indications for SDF testing were then formulated, and the Delphi method was used to reach consensus. RESULTS: The survey was completed by 436 experts from 55 countries. Almost 75% of respondents test for SDF in all or some men with unexplained or idiopathic infertility, 39% order it routinely in the work-up of recurrent pregnancy loss (RPL), and 62.2% investigate SDF in smokers. While 47% of reproductive urologists test SDF to support the decision for varicocele repair surgery when conventional semen parameters are normal, significantly fewer general urologists (23%; p=0.008) do the same. Nearly 70% would assess SDF before assisted reproductive technologies (ART), either always or for certain conditions. Recurrent ART failure is a common indication for SDF testing. Very few society recommendations were found regarding SDF testing. CONCLUSIONS: This article presents the largest global survey on the indications for SDF testing in infertile men, and demonstrates diverse practices. Furthermore, it highlights the paucity of professional society guideline recommendations. Expert recommendations are proposed to help guide clinicians.

Published
2023

47. Usefulness of Gastropanel for Validation of Efficacy of Drugs from Traditional Systems of Medicine in Functional Dyspepsia

Author

Sharada Hendre, Poonam Gupte, Supriya Bhalerao, Madhavi Mahajan, and Ajit S. Kolatkar

Subjects
General Medicine
Abstract

Gastropanel, serological ELISA test comprising of stomach biomarkers; serum pepsinogen I, pepsinogen II, gastrin-17, and Helicobacter pylori antibody depicts clearly the morphological and functional status of stomach mucosa in patients suffering from dyspepsia. Although the traditional system of medicine is a huge resource of efficient formulations useful in gastrointestinal disorders such as functional dyspepsia, lack of robust scientific evidence, and qualitative/subjective parameters like symptom scores do not suffice the need for the same. This study was thus planned to assess usefulness of gastropanel tests to validate efficacy of Avipattikar choorna, well-known antacid remedy in functional dyspepsia. A. choorna was given to patients of dyspeptic disorders following which gastropanel was performed pre- and post-treatment. The gastropanel findings obtained, prior and post-interventions were compared using Wilcoxon MPSR test and a level of P < 0.05 was considered for statistical significance. It was observed that although symptom scores showed improvement in all patients after treatment, change in pre- and post-values of gastropanel was seen only in few patients. Gastropanel could differentiate between true responders and non-responders, identification of which was difficult merely with symptom scores. Another highlight was that A. choorna proved more effective in H. pylori IgG positive cases. Gastropanel may be used as an effective tool to understand and validate the efficacy of traditional medicines in functional dyspepsia and gastric disorders along with its effect on stomach physiology and acid regulation. Need for employment of quantitative/objective parameters for traditional system drugs validation is also highlighted.

Published
2022

48. Clinical experience of total body irradiation performed on Halcyon™ for a patient diagnosed with acute myeloid leukemia

Author

Kaustav Talapatra, Anand Shamsunder Parab, Anuradha Singh, Shubhangi Barsing, Ajinkya Gupte, and Balkrishna Padate

Subjects
Pharmacology (medical)
Abstract

We aim to publish/document a clinically novel case of total body irradiation (TBI) performed on the Halcyon™. A 21-year-old gentleman diagnosed with high-risk tp53-positive acute myeloid leukemia planned for haploidentical bone marrow transplant post-chemotherapy following which he was declared minimal residual disease, negative was referred for TBI. The patient was placed in the supine position, arms by side with feet together. Immobilization was achieved using a full-body Vac-Lok. Two CT images of the cranial and caudal body parts were acquired. The PTV comprised the whole body minus 3 mm. The prescribed dose was 3 Gy in a single fraction. To achieve the desired dose distribution, we used 15 isocenters. Constraints were given to spare lungs and kidneys. The final plan was evaluated where 90% of the dose is received by 95% of volume, whereas lungs and kidneys received 80% of the prescribed dose, maximum dose of

Published
2022

49. Effect Of AJI-NO-MOTO On Kidney Of Adult Albino Rat: A Histopathological Evaluation

Author

Midhat Syed, Prenika Shangloo, Bonita Gupte, and Sangeeta Gupta

Subjects
General Earth and Planetary Sciences, General Environmental Science
Abstract

Introduction: Aji-No-Moto (MSG), the wonder flavoring agent, has been reportedly overused in all packed food products and the cuisines being served in restaurants. This salt effect almost all the organs of the body but the evidences regarding its ill effects are very limited. Thus, no guidelines are there for the safe limits of Aji-No-Moto use. In current study we planned to analyses the histopathological effects of Aji-No-Moto on kidney. Methods: The study was conducted on 18 inbred adult albino rats of either sex. The rats of control group (C) received only standard diet with distilled water, low dose test group (T₁) rats received 0.5mg/kg of MSG dissolved in distilled water and high dose test group (T₂) rats received 1.5mg/kg of MSG dissolved in distilled water per orally for 28 days. After the experimental period, the rats were sacrificed to dissect out the renal tissue which was later subjected to histological processing and tissue sectioning. Observations: The kidney tissue sections of the control group (C) revealed normal renal architecture consisting of cortical labyrinth and medulla. The cortical labyrinth consisted of glomeruli, PCT and DCT whereas the medulla consisted of the ascending and descending limbs of loop of henle and collecting ducts. On the other hand, renal sections of low dose group (T₁) showed focal shrinkage of renal glomerulus and widening of the Bowman’s space. Furthermore, these changes were more pronounced in high dose group (T₂) along with hypercellularity of glomeruli, dilatation, hyperaemia and congestion in the intertubular cortical blood vessels and mononuclear cell infiltrate. Conclusion: Aji-No-Moto is the most widely used flavoring agent whose minimal dose for use has to be evaluated. The current study was planned to access the minimal low dose limit of MSG for use. The results of aforementioned study revealed that even small dose of 0.5mg/kg/day is capable of producing histopathological effects on kidney.

Published
2022

50. Combinatorial Treatment with PARP-1 Inhibitors and Cisplatin Attenuates Cervical Cancer Growth through Fos-Driven Changes in Gene Expression

Author

Rebecca Gupte, Ken Y. Lin, Tulip Nandu, Jayanthi S. Lea, and W. Lee Kraus

Subjects
Cancer Research, Oncology, Molecular Biology
Abstract

Cervical cancer continues to be a significant cause of cancer-related deaths in women. The most common treatment for cervical cancer involves the use of the drug cisplatin in conjunction with other therapeutics. However, the development of cisplatin resistance in patients can hinder the efficacy of these treatments, so alternatives are needed. In this study, we found that PARP inhibitors (PARPi) could attenuate the growth of cells representing cervical adenocarcinoma and cervical squamous cell carcinoma. Moreover, a combination of PARPi with cisplatin increased cisplatin-mediated cytotoxicity in cervical cancer cells. This was accompanied by a dramatic alteration of the transcriptome. The FOS gene, which encodes the transcription factor Fos, was one of the most highly upregulated genes in the dual treatment condition, leading to increased Fos protein levels, greater Fos binding to chromatin, and the subsequent induction of Fos target genes. Increased expression of Fos was sufficient to hinder cervical cancer growth, as shown by ectopic expression of Fos in cervical cancer cells. Conversely, Fos knockdown enhanced cell growth. Collectively, these results indicate that by inducing FOS expression, PARPi treatment in combination with cisplatin leads to inhibition of cervical cancer proliferation, likely through a Fos-specific gene expression program. Implications: Our observations, which link the gene regulatory effects of PARPi + cisplatin to the growth inhibitory effects of FOS expression in cervical cancer cells, strengthen the rationale for using PARPi with cisplatin as a therapy for cervical cancer.

Published
2022

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