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By comparing and analyzing the solidification/stabilization technology of heavy metals in fly ash, it is found that the chemical stabilization method is simple in operation, low in energy eonsumption, and easy to realize the large-scale disposal of fly ash, but there is also a problem of expensive chemicals. If the chemical additive agent is combined w让h the mechanochemical method to dispose the heavy metals in fly ash, it can react with heavy metal compounds under the action of mechanical force, and generate insoluble heavy metal compounds to achieve the effect of stabilizing heavy metals. The disposal process has low energy consumption, less add让ive amount and can stabilize multiple heavy metals at the same time, which is expected to realize the harmless and resource utilization of fly ash on a large scale. In addition, the leaching risk of heavy metals in fly ash after disposal is easily affected by environmental factors, and the control mechanism of different leaching stages is also different. The eommonly used environmental Risk assessment code(RAC) only considers the proportion of mild acido-soluble fraction state when calculating the environmental risk of fly ash after disposal, which may lead to differences in the evaluation results. In order to accurately predict the environmental risk of fly ash after disposal, a combination of multiple evaluation methods to evaluate the environmental risk of fly ash after disposal may better reflect the accuracy of the evaluation results. The development of new effective and cost chemical stabilizers combined w让h meehanochemieal methods to treat heavy metals in fly ash is an important means to control heavy metals in waste incineration fly ash, and it is necessary to further improve the environmental risk assessment system of fly ash after disposal. [ABSTRACT FROM AUTHOR]

In order to improve the efficiency of domestic wastewater treatment process design, a wastewater treatment process database was established to save data samples abstracted from real domestic wastewater treatment processes, and the process was selected by the support vector machine classification algorithm based on boundary points(BP-SVM). Support vector machine classification algorithm based on boundary points(BP-SVM)was used to select the process. Multiple BP-SVMs were set up to solve the multi-classification problem. Parameter optimization of BP-SVM parameters was performed using genetic algorithm(GA). The results of the study show that the process design algorithm gives a suitable solution, verifies an accuracy of 94%, and consumes less compared to other algorithms. The feasibility and effectiveness of the algorithm is proved. [ABSTRACT FROM AUTHOR]

In order to understand the present situation of germplasm resources of different Cuora flavomarginata in Zhejiang Province, the genetic diversity and genetic structure of eight C. flavomarginata populations from wild and breeding farms in Zhejiang Province were compared based on mitochondrial DNA COI sequence. The results showed that the se⁃ quence length of COI gene of C. flavomarginata was 1，040 bp, and the average base content was A=27. 4%、T=30. 5%、C= 24. 6%、G=17. 5%, with strong AT preference. Nine haplotypes were identified in the eight populations, and the haplotype diversity (Hd) was (0. 79900±0. 21000), and the nucleotide diversity (Pi) was (0. 00209±0. 00147), showing“ high Hd and low Pi” genetic distribution. The genetic distance between different populations ranged from 0 to 0. 003. The genetic distance within populations was between 0 and 0. 004, among which the genetic distance within Pan’an Houge Village (YS) population was the largest, which was 0. 004. The haplotype phylogenetic tree did not show obvious pedigree structure. The genetic differentiation index (FST) interval was from -0. 34752 to 0. 82222, and the genetic differentiation was not significant (p>0. 05) in eight populations. Tajima’s D and Fu’s FS test values were either positive (Tajima’s D=-0. 36627, Fu’s FS=-0. 605), but statistically insignificant. The distribution of nucleotide mismatches indicated that the population of the C. flavomarginata in Zhejiang Province did not experience a population expansion event. Mantel test showed that there was no significant correlation between genetic distance and geographical distance (R=0. 2938，p>0. 05). AMOVA analysis found that the genetic variation within populations was higher than that between populations (85. 06%>14. 94%). The results showed that the populations of C. flavomarginata in Zhejiang Province are characterized by high haplotype diversity and low nucleic acid genetic diversity, and there was a certain level of genetic differentiation, and there was obvious gene exchange among the populations. The wild C. flavomarginata obtained in Pan’an County was identified as Zhejiang species according to COI gene analysis. Moreover, it was also found that there were hybrids between Zhejiang species and Anhui species. It is suggested that the C. flavomarginata populations with different degrees of differentiation should be divided into different units for protection. This study uses genetic diversity analysis to provide a basis for the development and utilization of C. flavomarginata germplasm resources in Zhejiang Province and the formulation of reasonable protection measures. [ABSTRACT FROM AUTHOR]

The research progress of benthic diatoms on sandy beach at home and abroad was reviewed in this paper. As the main flora of sandy beach, benthic diatoms adapted to the changeable sandy environment through a series of mechanisms. In the sediments of the intertidal zone, benthic diatoms were an important part of primary productivity and beach food webs. Sediment, temperature, salinity, light, nutrients and biological factors all affected the growth and community distribution of benthic diatoms. In recent years, there had been studies on the distribution characteristics of benthic diatom communities in the intertidal zone of China, but generally speaking, there were relatively few studies on benthic diatoms in sandy environment, and further investigation is needed to improve the study of diatom ecology in China. [ABSTRACT FROM AUTHOR]

Objective: To investigate symptoms of anxiety and depression among primary and middle school students quarantined in hotels during the COVID-19 epidemic. Methods: Anxiety and depression symptoms among 726 primary and middle school students quarantined in hotels were investigated with The Screen for Child Anxiety Related Emotional Disorders (SCARED) and Depression Self-rating Scale for Children (DSRSC) from September to October 2022 in Chifeng City, Inner Mongolia Autonomous Region. There were 624 students completed investigation with response rate of 86%. The positive score of SCARED was 23 and DSRSC was 15. Results: The detection rates of anxiety and depression were 17.9% and 15.4% respectively. The detection rates of anxiety and depression were higher in middle school students than in primary school students (Ps <0.05). The scores of generalized anxiety and social phobia factors were higher in female students than in male students (Ps <0.05). The scores of dissociative anxiety factor and depression were higher in middle school students than in primary school students (Ps <0.05). Conclusion: During the COVID-19 epidemic, middle school students quarantined in hotels are more likely to have anxiety and depression symptoms than primary school students, and female students are more likely to have anxiety symptoms than male students. [ABSTRACT FROM AUTHOR]

Objective To observe the clinical characteristics of benign congenital eustachian tube tumor in the middle ear in infants and the application of otic endoscope during the surgery. Methods A retrospective analysis of 3 patients with benign congenital eustachian tube tumor was performed. The clinical characteristics, radiologic characters, surgery, pathology and prognosis of all patients were recorded. All cases aged under 2 years and the course duration was between 20 days and 15 months. The main complaints were ear effusion in 2 cases and respiratory obstruction in 1 case. CT or magnetic resonance imaging (MRI) demonstrated the eustachian tube tumor. Hearing test showed a conductive hearing loss. All 3 patients got middle ear tumor resection surgery from retroauricular and auditory canal approach under microscope and endoscope. Results The lesion was originated from the eustachian tube, and the mass was exposed with the use of endoscopy. The surrounding large blood vessels could be identified, and there was no nerve or vascular damage during the operation. The postoperative pathological results confirmed 2 cases of choristoma and 1 case of teratoma. All patients were followed up for 1 to 4 years, and there was no recurrence. Conclusions Because of its special location, insidious onset and difficult operation, microscopy combined with endoscopic surgery could achieve better results and better prognosis. [ABSTRACT FROM AUTHOR]

Belt and Road countries and regions have increasingly recognized the urgent need for climate mitigation and adaptation in order to achieve the Paris Agreement and the Sustainable Development Goals (SDGs). Transferring and disseminating climate technologies are effective ways in which developing countries in the region can enhance their capacity to address the challenges of climate change. This study assesses the characteristics of climate change technology needed in Belt and Road countries and regions, based on the United Nations Framework Convention on Climate Change's Technology Needs Assessment (TNA) report as well as the Cooperative Patent Classification (CPC) system. It identifies and analyzes the priority needs for mitigation and adaptation technology needs in different areas. Results shows that climate technologies are in high demand in the Belt and Road region. A total of 726 climate technologies are included in the TNAs of 66 Belt and Road countries and regions, of which 262 are mitigation technologies and 464 are adaptation technologies. Almost all countries prioritize renewable energy for electricity generation (Y02E-10) for mitigation, with 135 technologies listed, covering Gross Domestic Production (GDP) of $5.3 trillion and a population of 1.9 billion. The most urgent need for adaptation technology is in agriculture, forestry, livestock, and fisheries production (Y02A-40), with 48 countries claiming 234 technologies for adaptation across this sector, covering $3.8 trillion in GDP and 1.61 billion in population. There is a high degree of heterogeneity in mitigation technology demands across regions. African countries are most in need of renewable energy generation technologies (Y02E-10), non-fossil fuel technologies (Y02E-50), and mitigation technologies for the land sector (Y02A-60). Asian countries are most interested in renewable energy generation and combustion technologies (Y02E-20), while Latin American and Oceania are most interested in mitigation technologies for road transport (Y02T-10). There is a high degree of commonality in the adaptation technology needs of all regions, with the priority needs of each region being primarily for adaptation technologies in agriculture, forestry, livestock and fishery production (Y02A-40) and technologies for harvesting, conserving, and efficiently using water (Y02A-20). In order to promote South-South technology cooperation between China and BRI developing countries, the proposed suggestions include: (1) better matching the technology supply by China with the demands in BRI countries and regions; (2) enhancing the transparency of technology information in China through various measures; (3) strengthening country-specific studies on climate technology needs and transfer strategy for BRI countries. [ABSTRACT FROM AUTHOR]

Objective A retrospective analysis of pediatric cases of total endoscopic ear surgery (EES) through the auditory canal was made to explore the indications, preoperative evaluation, and key points of intraoperative application of EES in children’s middle and inner ear surgery. Our purpose was to promote the application of the visualized and minimally invasive technology in children’s middle and inner ear surgery. Methods A retrospective analysis of the patients with EES from Beijing Children’s Hospital from January 2016 to January 2021 was made. The patients’ age, disease name, preoperative temporal bone CT findings, hearing, intraoperative findings, and postoperative follow-up were included. The key points of preoperative evaluation and the clinic paths were summarized. Results A total of 123 cases (123 ears) of EES were collected, including 70 cases (70 ears) of tympanoplasty, 30 cases (30 ears) of middle ear cholesteatoma resection, and 23 cases (23 ears) of cerebrospinal fluid otorrhea repair. The minimum age was 1 year and 1 month, and the maximum age was 17 years. The median age for tympanoplasty was 8 years and 5 months. The median age for middle ear cholesteatoma resection with ossicular chain reconstruction was 4 years and 3 months, and the median age for cerebrospinal fluid (CSF) otorrhea repair was 4 years and 2 months. The transverse diameter of the external ear canal (EAC) in children undergoing tympanoplasty was 5.0~7.15 mm, and the longitudinal diameter was 4.5~6.5 mm. The transverse diameter of the EAC in children undergoing middle ear cholesteatoma resection was 4.6~6.0 mm, and the longitudinal diameter was 4.5~6.5 mm. The transverse diameter of the EAC in patients with CSF otorrhea was 4.5~6.0mm, and the longitudinal diameter was 4.5~6.5 mm. Children with middle ear cholesteatoma had lesions limited to the middle tympanic cavity. Five cases had lesions limited to the upper tympanic cavity. Ten cases of lesions located in both the middle and upper tympanic cavities. Ten cases involved the superior recess of the eustachian tube and/or the tympanic sinus. Six children with tympanic sinus cholesteatma underwent intraoperative combined use of 0 ° and 70 ° endoscopes. The fistula of CSF otorrhea repair surgery was located at and around the vestibular window. After removing the stapes, varying degrees of bone defects could be seen on the base plate.Tympanoplasty classification: 66 cases of type Ⅰ, 1 case of type Ⅱ, and 3 cases of type Ⅲ. The postoperative hearing result of patients undergoing middle ear cholesteatoma resection or tympanoplasty was better than before(P＜0.001). Two (2/66) children with type Ⅰ tympanoplasty had small perforations of the tympanic membrane six months after surgery, and the remaining patients had good healings of the tympanic membranes. There had been no recurrent cases of cholesteatoma. Two cases of CSF otorrhea underwent secondary endoscopic closure due to the recurrence of otorrhea after surgery, and no recurrence happened again. Conclusions EES in children could be suitable for middle and inner ear surgery and might achieve good prognosis. Preoperative evaluation of ear canal width, lesion range, and leak location was the guarantee for a successful EES [ABSTRACT FROM AUTHOR]

Microbially Induced Carbonate Precipitation (MICP) technology is widely used in the control of soil heavy metal pollution. However, the composition of the soil can reduce the nucleation rate of calcium carbonate, in order to solve the problems of slow nucleation during its mineralization and unstable crystalline form of mineralization products. In this study, six regulatory factors were selected: sodium citrate (C6H5Na3O7), magnesium oxide(MgO), magnesium chloride (MgCl2), chitosan ((C6H11 NO4)n), ethanol (C2H5OH) and silicon dioxide (SiO2). The results showed that sodium citrate (TKa) was able to accelerate the crystallization and produce the highest amount of precipitation, and the removal rate of lead reached 90%. The results showed that sodium citrate stabilized the sphalerite, accelerated the mineralisation process and increased the mineralisation precipitation to improve the removal of heavy metals. Therefore, sodium citrate has the ability to increase the rate of MICP bioremediation, which is important for improving the efficiency of MICP technology application. [ABSTRACT FROM AUTHOR]

As the dividing line of the political entity's jurisdiction, the border causes the discontinuity of social and economic, and then forms political, economic and cultural differences. The border is a key interface for the free cross-border flow of elements, and its shielding or mediating effect affects the welfare level of a country or region's social and economic development. Using the literature review method, this paper sorted out the origins and basic concepts of the border effect, summarized the development context of the border effect from measurement method, research scale and analysis conclusion, and discussed the research perspective and main content of border effect in Economic Geography, World Economic, Regional Economics and Industrial Economics. Based on the heterogeneity of the research perspective, five main measurement methods of border effect were summarized, namely, the gravity model, the law of one price, the Ricardian model, the computable general equilibrium model and the regional growth model. Finally, this paper discusses the future research direction of border effect based on China's practical problems: exploring the countermeasures to overcome the implicit border barrier, strengthening the research on compensation modes of border effect in border areas, and exploring the collaborative optimization mechanism of administrative boundaries at all levels, to promote the high-quality development of China's economy. [ABSTRACT FROM AUTHOR]

Objective To investigate the application value of conventional MRI signal intensity ratio (SIR) in differential diagnosis of glioblastoma (GBM) and primary central nervous system lymphoma (PCNSL) and the correlation with Ki-67 expression. Methods Retrospective analysis of 72 cases of GBM and 32 cases of PCNSL MRI data, t test were used to analyze signal intensity ratio of tumors, four indexes were included: signal ratio of T1WI (rT1), signal intensity ratio of T2WI (rT2), enhanced signal ratio of T1WI (rT1CE) and signal ratio of T2WI/T1WI (T2/T1). The differential diagnostic performance was evaluated by receiver operating characteristic (ROC) curve. Spearman test was used to analyze the correlation between SIR and (Ki-67 label index, Ki-67 LI). Results The rT1 and rT1CE (rT1: 0.482±0.117, rT1CE: 1.319±0.243) of GBM were significantly lower than that of PCNSL (rT1: 0.557±0.478, rT1CE: 1.594±0.156), while rT2 and T2/T1 (rT2: 1.814±0.285, T2/T1: 3.995±1.189) were significantly higher than that of PCNSL (rT2: 1.435±0.135,T2/T1: 2.600±0.354), the comparison between the two groups was statistically significant (P＜0.001). ROC curve analysis showed that rT1, rT2, rT1CE and T2/T1 could be used to distinguish GBM from PCNSL. A single parameter of T2/T1 had the best diagnostic efficacy (AUC: 0.877), with a sensitivity of 93.8% and a specificity of 76.4%. rT1 +rT1CE +rT2 +T2/T1 combined parameters had the highest differential diagnostic efficiency (AUC: 0.966), with sensitivity of 87.5% and specificity of 91.7%. Correlation analysis showed that rT1 and rT1CE were positively correlated with Ki-67 LI, the correlation coefficients were 0.301 and 0.399, while rT2 and T2/T1 were negatively correlated with Ki-67 LI, and the correlation coefficients were-0.428 and-0.459 respectively. Conclusion Tumor SIR in MRI is important for discriminating GBM from PCNSL, and SIR as a quantitative parameter can provide an important complement to subjective judgment and preliminarily evaluation for the proliferation level of tumor cells. [ABSTRACT FROM AUTHOR]

The command control (C2) is the most important part of the war for all dynasties. In information age, the future battlefield poses new challenges for C2. By analyzing the characteristics of network enabled command and control in the information age, this paper studies the C2 function and approach space, defines five C2 archetype, discriminates in detail the objectives and requirements of each archetype, analyzes its maturity level and maturity migration model, and finally discusses the challenges to C2 in the future battlefield. It is important to help the combatant analyze the problems existing in current C2, to improve the C2 ability of the current and future, to improve the control of the commanders, and to improve the manage ability of complex, dynamic and uncertain combat missions. [ABSTRACT FROM AUTHOR]

In order to reduce the temperature stress caused by the large temperature difference inside and outside the anchorage mass concrete structure, the fly ash and the self-developed hydration inhibitor were mixed into the concrete. The effects of hydration inhibitor on the temperature-shrinkage deformation of mass concrete were investigated. As a result, the hydration inhibitor can effectively reduce the loss of cement fluidity and prolong the setting time of cement by more than 4 hours. The early hydration temperature of mass concrete decreases with the increase of the content of fly ash and hydration inhibitor, and the peak value of hydration temperature moves backward. The micro-strain of mass concrete decreases with the increase of fly ash and hydration inhibitor, and the inhibition effect of hydration inhibitor on hydration is more obvious at lower content. The shrinkage value of concrete without fly ash in 7 d has exceeded 70% of the age of 180 d, while the shrinkage value of concrete with fly ash in 7 d is only about 50% of the age of 180 d, and the shrinkage value of 180 d is 70% in 28 d. On this basis, it takes 45 d for concrete with hydration inhibitor to reach 70% of the shrinkage of 180 d. Considering the comprehensive effect and cost, it is recommended to use 40% fly ash and 0.2% hydration inhibitor to prepare anchorage mass concrete. 40% fly ash and 0.2% hydration inhibitor to prepare Anchorage mass concrete. [ABSTRACT FROM AUTHOR]

Objective·To evaluate the efficacy of the preservation of alveolar crest in situ for regeneration of periodontal osseous defect distal to the mandibular second molar (M2) after mandibular third molar extractions. Methods·After screening by evaluation, a total of 19 patients were enrolled and accepted the mandibular third molar extractions with modified ridge preservation at Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from August 2020 to August 2021. Meanwhile, 8 cases of traditional tooth extraction were included in the control group. All the patients were followed up at 1 week, 3 months and 6 months after operation. Postoperative symptoms including pain, swelling and nerve injury were also recorded in detail. Intraoral clinical examination was performed on M2, including cold and heat stimulation, tooth percussion results and looseness. Osseous defect depth (ODD) on the disto-buccal aspect of M2 was taken as the primary outcome variable. Probing depth (PD) and clinical attachment level (CAL) were taken as the secondary outcome variables during the follow-up period. In the meantime, all the patients received cone beam computed tomography (CBCT), and the data were analyzed and reconstructed for the measurement of ODD through CS3DImaging software. Results·No obvious postoperative complications such as infection or never injury were found in any patient. The PD, CAL and ODD on the disto-buccal aspect of M2 at 6 months after operation in the two groups were both improved compared with those at 3 months after operation (P<0.05 for all). Furthermore, all the data in the experimental group were lower than those of the control group at any time of the follow-up (P<0.05 for all). Conclusion·Modified ridge preservation has a notable improvement in regeneration of periodontal osseous defect distal to the M2 after mandibular third molar extractions. [ABSTRACT FROM AUTHOR]

Objective To better carries out the review of the interaction between triazole antifungal drugs and statins, ensure drug safety for patients and improve the efficiency of the manual review of pharmacists. Methods The advantageous combination with less interaction between the two kinds of drug was obtained by summarizing the interaction information of triazole antifungal drugs and statins. At the same time, optimizing the process of full manual reviews for drug interaction, and the advantageous combination of the two kinds of drug will be set up in advance on the doctor side of the review system. Doctors can adjust the drug in time according to the prompts of the information system when there is a significant interaction of the two kinds of drug in the prescription or medical order, which is issued by the doctors. Results After optimization, most doctors choose the drug combination of less interaction between the two kinds of drug according to the system prompts. The number of drug combinations of significant interaction which is manually reviewed by pharmacists is significantly reduced (P<0.05), and the review efficiency is improved, the drug safety risks brought by drug interactions to patients are reduced. Conclusion Refinement review of drug interactions between triazole antifungal agents and statins based on the optimization of the pre-audit system plays a positive role in improving the safety of patients’ medication and the efficiency of pharmacists' manual auditing. [ABSTRACT FROM AUTHOR]

报道成都市第五人民医院收治的1例初诊断2型糖尿病（T2DM）患者入院予以胰岛素强 化降糖后，转换为利拉鲁肽联合口服降糖药物治疗的诊疗经过。患者为54岁女性，因“口干、多饮、多 尿伴消瘦3个月”入院，入院后完善各项相关检查，明确诊断为T2DM。初始启用胰岛素强化降糖，血 糖平稳后转换为利拉鲁肽皮下注射联合二甲双胍口服控制血糖，患者血糖控制良好，无明显胃肠道不 良反应。利拉鲁肽是一种胰高糖素样肽‑1受体激动剂，可以联合口服降糖药物及胰岛素控制血糖，降 糖效果较好，特别是针对伴有肥胖的患者，同时还能带来心血管、肾脏方面的获益。 [ABSTRACT FROM AUTHOR]

Shanghai has been carrying out many municipal land consolidation projects to improve the intensive use of land, increase newly cultivated land, protect cultivated land, and ensure national food security. The landscape pattern of cultivated land is a direct reflection of cultivated land agroecosystems. Based on a municipal land consolidation project in Shanghai, this study selected three typical administrative villages (Village C, L, and N) to analyze the relationship between cultivated land quality and cultivated land landscape patterns at the village scale, using spatial autocorrelation analysis and a geographically weighted regression model. This study provides a theoretical reference for farmland planning for land consolidation projects. The results showed that the cultivated land quality of the three villages was strongly affected by patch density (PD), area-weighted average shape factor (AWMSI), maximum patch area (LPI), and Shannon diversity (SHDI). Village A had the best– cultivated land quality. Although the degree of cultivated land fragmentation was higher than that of Villages L and N, Village C had the advantage of rich landscape ecology and high connectivity. The degree of fragmentation of cultivated land in Village C was mainly affected by the cutting effect of agricultural infrastructure, such as ditches and roads. Modern agriculture was developed in Village C. Reasonable planning of farmland landscape elements such as roads and ditches and improvement of cultivated land quality from the perspective of optimizing landscape patterns can effectively promote the coordinated development of cultivated land production and ecological function. [ABSTRACT FROM AUTHOR]

To introduce a new flap design method which can be applied in the fingertip replantation of Wassel type VI polydactyly. Methods There were 3 operations in the Department of Plastic Surgery of Fujian Maternal and Child Health Hospital from January 2015 to October 2019. The lingual flap was designed on the radial side of thumb and the corresponding incision was designed on the ulnar side of thumb during the operation. The blood supply of distal phalanx and proximal phalanx was provided by ulnar digital artery. The new thumb was reconstructed by combination of the broken ends of bone, fixation of K-wire and tendon transfer. After the K-wire was removed at 4 to 6 weeks after operation, active and passive functional exercises should be carried out. Results All patients were followed up for 12 to 24 months, with an average of 16.67 months. Good efficacy was achieved. The stability and mobility of thumb were close to those of healthy finger. All parents were satisfied with the postoperative function and appearance. Conclusion The new design method can provide a new idea for fingertip replantation of Wassel type Ⅵ polydactyly. Long follow-up is needed to verify the final results. [ABSTRACT FROM AUTHOR]

A method for glycosides detection in tobacco leaves by heart-cutting Rp/Rp two- dimensional liquid chromatography-tandem mass spectrometry was established. Three glycosides were determined, including 3-oxo-7, 8-dihydro-α-ionol-,β-D-glucopyranoside, 3-oxo-α-ionol-, β-D-glucopyranoside and 3-hydroxy-, β-dihydro-damascone-pi-β-glucopyranoside. Only extraction and filtration were required in sample pretreatment. The RSDs of intra-day and inter-day were in the range of 1. 0 % -3. 2 % and 2. 8 % -8. 4 %, respectively, which showed good reproducibility. Since sample pretreatment was very simple, the method was quite suitable for daily detection. Different types of tobacco were determined, including Virginian-type tobacco, Burley tobacco, oriental tobacco and sun-cured tobacco. The results revealed that glycosides influecured and fresh Virginian-type tobacco were significantly higher than other types, which agreed well with previous reported articles. 157 kinds of Virginian-type tobacco samples from different regions were monitored and investigated. Among the factors, which influenced contents of glucosides in Virginian-type tobacco, sugar was no longer the major one, catabolite of carotenoids (C13-isoprenol glucosides) and temperature were also considered an important factor in our study. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To probe into the effect of Dangua Humai oral liquid on vascular endothelial function and oxidative stress indicators in patients with type 2 diabetes mellitus (T2DM) complicated with non-alcoholic fatty liver disease ( NAFLD) under the guidance of co-treatment of phlegm and stasis. METHODS: Totally 92 patients with T2DM complicated with NAFLD admitted into the Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine from Jan. 2018 to Jul. 2020 were selected and divided into control group ( 46 cases) and observation group ( 46 cases) according to the admission sequences. The control group was given conventional therapy, while the observation group was given Dangua Humai oral liquid based on the control group under the guidance of co-treatment of phlegm and stasis, both groups were treated for 3 months. The clinical efficacy, blood glucose [fasting blood glucose( FBG), 2 h postprandial blood glucose ( 2 hBG) and glycosylated hemoglobin ( HbA1 c) J levels, blood lipid [ total cholesterol ( TC), triglyceride ( TG), high-density lipoprotein ( HDL ) and low-density lipoprotein ( LDL) ] levels, liver function indicators [alanine aminotransferase (ALT), aspartate transaminase ( AST) and y - glutamyltransferase ( GGT) J, vascular endothelial function indicators [vascular endothelial growth factor ( VEGF ), endothelin (ET-1) J and oxidative stress indicators [malondialdehyde (MDA), superoxide dismutase ( SOD) J levels were compared between two groups before and after treatment. RESULTS: The total effective rate of observation group was 89. 13% ( 41/46), which was significantly higher than that of the control group ( 71. 74%, 33/ 46 ), with statistically significant difference ( P <0. 05) . After treatment, the FBG, 2 hBG, HbA1 c, TC, TG, LDL, ALT, AST, GGT, ET-1 and MDA levels of both groups had been significantly decreased, while the HDL, VEGF and SOD levels were significantly increased, with statistically significant differences (PO. 05 ), the 2 hBG, TC, TG, LDL, ET-1 and MDA levels of observation group were significantly lower than those of the control group, while the HDL, VEGF and SOD levels were significantly higher than those of the control group, with statistically significant differences ( P < 0. 05) . CONCLUSIONS: Under the guidance of co-treatment of phlegm and stasis, Dangua Humai oral liquid can better regulate blood lipid and blood glucose metabolism in patients with T2DM complicated with NAFLD, improve their vascular endothelial function, reduce oxidative stress and promote therapeutic effect, without significant adverse drug effects. [ABSTRACT FROM AUTHOR]

A method for the determination of 50 pesticide residues such as dichlobenil, fluxapyroxad, fluopicolide, metrafenone, etc, in dry chilli was established using solid phase extraction (SPE) combined with gas chromatography-tandem mass spectrometry (GC-MS/MS). After being soaked in water, the samples were extracted with acetonitrile, salted out with sodium chloride, and then centrifuged. The supernatant was cleaned-up by SPE column of CARB/NH2, then dissolved and diluted to 1.0 mL with ethyl acetate. The targeted compounds were detected by GC-MS/MS and then quantified by the matrix matched external standard calibration method. The results showed that among the 50 pesticides, 46 analytes displayed good linearity in the range of 0.005-0.5 mg/L, with determination coefficient (R² ) > 0.99. The limits of quantitation (LOQ) ranged from 0.01 to 0.1 mg/kg. The average recoveries for the 46 pesticides at spiked levels of 0.01, 0.1, 1.0 mg/kg in dry chilli were in the range of 72%-120%, and the relative standard deviations (RSD) were between 0.7% and 17%. The method is simple, rapid, highly efficient, and the sensitivity, accuracy, and precision meet the daily routine testing requirements. It is suitable for the simultaneous detection of multiple pesticide residues in dry chilli samples. [ABSTRACT FROM AUTHOR]

Human activities have dramatically changed the subsurface flow and caused problems such as salinization and desertification that degrade groundwater-dependent the ecosystem. Taking the shallow groundwater layer as the link, a conceptual model of phreatic water evaporation in arid plains is established. The model analyses the groundwater depth conditions of desertification and salinization in arid areas, and calculates threshold water table required for preventing these problems. The Luocheng Irrigation District at the junction of the middle and lower reaches of Heihe River is taken as an example to study the relation between groundwater depths and ecosystems patterns. The contributions of this study include: ① Describing the soil water movement principle caused by desertification outside the salinized oasis in the arid area, based on which, the method for calculating the threshold groundwater depth for preventing salinization is provided. By calculation, the critical groundwater depth leading to soil salinization is 1.3~1.5 m and the calculated critical groundwater level is 8~13 m; ② Discussing the appropriate values of soil equivalent pore diameter and liquid surface tension used for estimating the maximum rising height of capillary water; ③ Proposing a scheme to regulate the groundwater level regulation via controlling irrigation in the area to protect groundwater-dependent ecosystems while satisfying water demand for irrigation and other purposes. [ABSTRACT FROM AUTHOR]

[Objective] By analyzing the expression of CmGolS1 (galactinol synthasel) and the changes of soluble sugar content in Miben' pumpkin under drought stress, the physiological, biochemical and gene level changes of pumpkin under drought stress were analyzed, so as to improve the drought tolerance of pumpkin. [Method The Cucurbita cultivar Miben' was selected as the research material and treated with drought for 2-7 days. The changes of CmGolS1 gene expression and soluble sugar content were detected by fluorescence quantitative PCR and soluble sugar content assay. [Result] CmGolS1 was almost not expressed in the control group, but with the progress of drought treatment, its expression level increased significantly, reached a significant high value on the third day of drought treatment, and then fluctuated within 3-7 days of drought treatment, but remained high compared with the control group. The soluble sugar content of the first true leaf of pumpkin in the control group was maintained at about 220 µg/g; it began to increase significantly at 3 days after drought treatment, and then maintained at 320-330 µg/g. The soluble sugar content remained relatively stable in the middle and late stage of drought treatment. [Conclusion] Under drought stress, the expression of CmGolS1 is up-regulated, and its synthetic product galactose is the precursor of a variety of soluble sugars, which may be related to the up regulation of soluble sugars in physiology and biochemistry. The accumulation of soluble sugar is an important physiological response in the process of plant drought resistance, which helps to improve cell water potential and resist drought environment. CmGolS1 gene can provide important information for physiological and biochemical changes of pumpkin under drought stress and molecular mechanism of drought resistance breeding. [ABSTRACT FROM AUTHOR]

Objective To investigate the impact of exosomes derived from adipose tissue mesenchymal stem cells (ADSCs-exo) on dendritic epidermal T cells (DETC) and inflammation in cutaneous wounds of mice. Method From January to December 2023, ADSCs were isolated using the type I collagenase digestion method, while ADSCs-exo were obtained through differential ultracentrifugation. DETCs were extracted and cultured in primary culture, with cell purity confirmed by flow cytometry. An inflammation model of DETC cells was established in vitro using cleaved soybean protein A (ConA), and the mRNA expressions of IL-2 and IL-17A in cells were analyzed by RT-qPCR. A full-thickness skin defect wound model was created on the back skin of C57 mice, which were then divided into a blank control group, a PBS injection group, and an ADSCs-exo injection group. The levels of IL-17A, Akt, and P-Akt at the wound edge were assessed by Western blotting. The expression of DETCs at the wound edge was observed via flow cytometry. Results In the DETC in vitro inflammation model, the mRNA expressions of IL-2 and IL-17A were significantly elevated in the ConA group compared to the Control group. However, in the ConA+ADSCs-exo groups, there was a noticeable decrease in mRNA expressions of IL-2 and IL-17A when compared to the ConA group. During the mouse wound healing process, lower levels of IL-17A content were detected at the skin edge of wounds treated with ADSCs-exo compared to those treated with PBS or left untreated (control). Additionally, higher expression levels of P-Akt were observed at these sites as well. On day one after acute trauma, both PBS and ADSCs-exo treatments resulted in significantly increased migration and enrichment degree of DETC cells at wound edges compared to control samples. By day three post-injury, while DETC expression remained significantly higher for PBS-treated wounds relative to controls; its expression decreased within ADSCs-ex-o-treated wounds and reached similar levels as seen in control samples. Conclusion ConA can stimulate activated DETC to secrete IL-17A and IL-2, whereas ADSCs-exo significantly suppress the expression of IL-2 and IL-17A. During the process of wound healing in mice, ADSCs-exo inhibit the expression of IL-17A while promoting P-Akt expression. The expression of DETC at the wound site can be upregulated in response to acute trauma; although ADSCs-exo do not exhibit obvious chemotactic enrichment effect on DETC, the inhibition of DETC expression effectively impedes the occurrence of persistent and excessive inflammatory reactions in the wound, thereby ensuring a more controlled and regulated healing process. [ABSTRACT FROM AUTHOR]

Objective To explore the effectiveness of automatic detection, segmentation and quantitative analysis of melanin nevi in whole-face images in multi-center clinical trials. Methods Logistic regression and Newton method were used to detect the nevus area based on the whole-face images of patients from January to June 2019. Python and OpenCV were used to detect the lesion edge and calculate the area. The effectiveness of the algorithm was evaluated in multi-center clinical trials. Results The algorithm detected 1290 nevi in 300 patients, 80 missed diagnosis and 125 misdiagnosis. The recall rate was 93.57%, the accuracy rate was 90.31%, and the F value was 0.92. Kappa value between two groups was > 0.8; Paired t test showed no significant difference in area between two groups (P=0.720, P> 0.05). Conclusion Within the limitations of this study, we demonstrate that the algorithm's detection is highly consistent with the physician's diagnosis. The algorithm has achieved good results in detection of nevus, edge segmentation and area measurement. [ABSTRACT FROM AUTHOR]

目的了解医院护士长关怀行为现状,探讨护士长关怀行为与护士工作满意度的相关性,为制定护士长关怀行为 标准提供依据.方法 采用护士工作满意度量表和护士长关怀评价量表对自贡市某三甲医院 763 名在职护士进行问卷调查。结 果护士长关怀评价得分为(112.70±18.00)分,处于中等水平;婚姻状况、月平均夜班数、所在科室是护士工作满意度､内在满意 度維度及外在滿意度維度的主要影响因素;月平均夜班数是护士长关怀评价总分、工作关怀与帮助维度､生活关怀与帮助维度､信 任与支持维度､尊重与理解维度,重視与鼓励维度的主要影响因素。结论护士长关怀对护士人文关怀能力及工作滿意度有机械 作用,护理管理者应加强对护士的关怀,提高护士关怀能力及工作满意度,营造和谐护患关系,提高患者满意度。 [ABSTRACT FROM AUTHOR]

目的 探究 1 型糖尿病（T1DM）患者胰岛自身抗体与甲状腺抗体的相关性。方法 选取 T1DM 患者 55 例 为观察组及同期健康体检人群60例为对照组。两组禁食10 h以上，于次日清晨抽取空腹静脉血 5 mL，采用化学发 光免疫分析法检测胰岛细胞抗体（ICA）；采用化学发光法检测胰岛素抗体（IAA）、谷氨酸脱羧酶抗体（GADA）；采用 化学发光免疫夹心分析法检测甲状腺功能指标FT3、FT4、TSH、甲状腺过氧化物酶抗体（TPOAb）和甲状腺球蛋白抗 体（TGAb）。比较两组甲状腺功能异常情况，胰岛自身抗体 ICA、IAA、GADA 及甲状腺自身抗体 TPOAb、TGAb阳性 率，分析 T1DM 患者胰岛自身抗体与甲状腺抗体的相关性。结果 观察组甲状腺功能异常发生率高于对照组 （23. 64% vs 6. 67%，P<0. 05）；观察组IAA、GADA任一抗体阳性率高于对照组（40. 00 % vs 1. 67%），TGAb阳性率高 于对照组（18. 18% vs 3. 33%），TPOAb 阳性率高于对照组（21. 82% vs 9. 09%），差异均有统计学意义（P 均<0. 05）。 观察组 IAA、GADA 阳性患者 TGAb、TPOAb 阳性率高于相应阴性患者（P 均<0. 05）。结论 T1DM 患者甲状腺功能 异常及甲状腺抗体阳性率高于健康人群，GADA、IAA阳性T1DM患者伴发自身免疫性疾病的风险更高。 [ABSTRACT FROM AUTHOR]

To explore the effects of different fe1tilization formulas on the growth of large-diameter timber of a Chinese fit( Cunninghamia lanceolata) plantation, 12 fe1tilization treatments, including "3414" incomplete treatment (potassium fixation), soil testing formula, tree testing formula, and microelement balance formula, were selected to analyze and compare the diameter structure, large diameter timber output, and timber yield of a Chinese fir plantation under different fe1tilization treatments. The results provide scientific references for selecting the best fertilization amount and formula for the cultivation of large-diameter timber of Chinese fir. Our results showed that after 4 years of fertilization, the number of large-diameter timber in Chinese fir plantations was the highest, accounting for 53.33% of the total number. Treatment 10( N-soil P-soil K-soil) resulted in the largest increase in timber output, reaching 199.64 m3 ·hm-2, which was 63.77% more than the control treatment l(N0 P0 K0 ). Treatment 10 and treatment 8(N3P2K2 ) resulted in higher timber yields and growth than the other treatments. Among them, treatment 10 resulted in the highest timber yield of 70.65%, and treatment 8 the largest increase in growth of 17.28%. These results showed that soil testing and fe1tilizer treatment 10 had the best effect on large-diameter timber cultivation of Chinese fir near a mature forest in the sample area when the application rates of nitrogen, phosphorus, and potassium were 140, 500, and 60 g per plant, respectively. There was no significant difference in timber yield among the treatments in a short time. The fe1tilization scheme of treatment 8 followed by treatment 10 indicated that the combination of higher concentrations of nitrogen fertilizer and moderate concentrations of phosphorus fertilizer was also an effective means to promote the cultivation of large-diameter timber of Chinese fit near a mature forest. [ABSTRACT FROM AUTHOR]

Objective To investigate the repair effect of bone marrow mesenchymal stem cells (BMSCs) on pulmonary vascular endothelial injury in rats with chronic obstructive pulmonary disease (COPD) -obstructive sleep apnea (OSA) overlap syndrome and to explore its possible mechanism. Methods BMSCs were isolated from femur and tibial bone marrow of SPF-grade female SD rats, and then were cultured and identified. Sixteen female SD rats with SPF grade were selected to prepare the RAT models of COPD-OSA overlap syndrome by smoking combined with intermittent hypoxia. The rats were randomly divided into the observation group and control group, with 8 rats in each group. The rats in the observation group were injected with 2 × 106 BMSCs per time, once a week, for 4 times in total, and the rats in the control group were injected with normal saline of the same volume. One week after treatment, the rats in the two groups were sacrificed, the lung tissues were taken for paraffin section for HE staining and MASSON staining to observe the pathological changes of the lung tissues and pulmonary arteriole, and the apoptosis of vascular endothelial cells in the lung tissues was observed by the triple qualitative method of DAPI-CD34-TUNEL,the expression levels of endothelin 1 (ET-1), endothelial nitric oxide synthase (e NOS), vascular endothelial growth factor (VEGF) and stromal cell derived factor 1 (SDF-1) were detected by ELISA. Results Compared with the control group, the pathological changes of the lung tissues in the observation group were milder, such as emphysema, interstitial lymphocyte infiltration, neutrophil infiltration, bronchial wall lymphocyte hyperplasia, bronchiolar wall smooth muscle hyperplasia and goblet cell hyperplasia; the changes of pulmonary arterioles myosization, outer membrane collagen fiber deposition, abnormal vascular wall structure, perivascular inflammation, and pulmonary interstitial thickening were relatively mild. The apoptosis rates of pulmonary vascular endothelial cells in the observation group and the control group were 2. 3552% ± 0. 3923% and 1. 3339% ± 0. 1553%,respectively,with statistically significant difference (both P ＜ 0. 01). The content of ET-1 and VEGF in lung tissue homogenization of the observation group was lower than that of the control group, and the content of e NOS was higher than that of the control group, with statistically significant difference (all P ＜ 0. 05); the SDF-1α content in lung tissue homogenization in the observation group was lower than that in the control group, but no statistically significant difference was found between these two groups (P ＞ 0. 05). Conclusions BMSCs can improve the vascular endothelial injury and dysfunction in pulmonary tissues of rats with COPD-OSA overlap syndrome, reduce the apoptosis of pulmonary vascular endothelial cells, and improve the remodeling of pulmonary tissues and small pulmonary vessels to some extent. [ABSTRACT FROM AUTHOR]

Microbilly induced calcite precipitation (MICP) was used to stabilized Pb by Bacillus pasteurii to investigate the remediation effects in polluted soil. The leaching rate of Pb in soil decreased by 76, 34 %，the content of available state decreased by 41. 64% ? and the content of carbonate binding state increased by 13.24% by the bacterium due to its co precipitation with calcium carbonate. Pb were co precipitated with calcium carbonateon thesurface of soil particles according to SEM and XRD analysis. MICP technology has great applicationprospectson the remediation of Pb pollution soil. [ABSTRACT FROM AUTHOR]

Comprehensive scoring models are one of the main methods for liver function evaluation and an important part of the treatment strategy for hepatocellular carcinoma. This article summarizes the value and limitations of traditional scoring models(Child-Turcotte-Pugh score and Model for End-Stage Liver Disease score) and new scoring models(albumin-bilirubin score,platelet-albumin-bilirubin score,and albumin-indocyanine green score) in radiofrequency ablation for hepatocellular carcinoma and points out that the new scoring models,especially platelet-albumin-bilirubin score,are advantageous. [ABSTRACT FROM AUTHOR]

BACKGROUND: The combined action of mechanical compression, inflammatory factors, and matrix metalloproteinases leads to intervertebral disc degeneration. Rhizoma drynaria flavones have been shown to have anti-inflammatory effect. However, its protective effects against degeneration of the intervertebral discs remain unclear. OBJECTIVE: To investigate the potential molecular mechanisms underlying the protective effects of rhizoma drynaria flavones in a rat model of cervical intervertebral discs degeneration and analyze the clinical value. METHODS: Fifty Sprague-Dawley rats (half male and half female) were randomly divided into two groups: 10 in sham-operated group and 40 in degeneration group. Animal models of cervical intervertebral discs degeneration due to dynamic and static imbalance were made in the degeneration group. After successful modeling, 36 model rats were randomized into model group, high-dose group, middle-dose group and low-dose group (n=9 per group). Rats in the sham and model groups were intragastrically given 4 mL of normal saline per day, and those in the high-, middle- and low-dose groups were given 62.5, 125, and 250 mg/kg rhizoma drynaria solution, respectively. The C3-4, C4-5, C5-6, and C6-7 segments of the rat spine were taken. Immunohistochemistry and ELISA were used to detect expression of tumor necrosis factor α and matrix metalloproteinase 3 proteins. Quantitative RT-PCR was used to determine the mRNA expression of tumor necrosis factor α and matrix metalloproteinase 3. Western blot was used to detect the phosphorylation of IkBα. RESULTS AND CONCLUSION: Results from the immunohistochemistry, western blot, and RT-PCR, and ELISA showed that the phosphorylation of IkBα and expression of tumor necrosis factor α and matrix metalloproteinase 3 were significantly higher in the model group than the sham group (P < 0.01). However, interventions with rhizoma drynaria flavones, especially with low-dose rhizoma drynaria flavones, significantly lowered the phosphorylation of IkBα and expression of tumor necrosis factor α and matrix metalloproteinase 3. To conclude, high-,middle- and low-dose rhizoma drynaria flavones can exert protective effects against degeneration of the intervertebral discs by suppressing the nuclear factor-κB pathway and inhibiting the expressions of tumor necrosis factor α and matrix metalloproteinase 3. And low-dose rhizoma drynariae flavonoids have the optimal outcomes. [ABSTRACT FROM AUTHOR]

Objective To investigate the clinical value of acoustic radiation force impulse (ARFI) elastography in predicting esophageal variceal bleeding in patients with liver cirrhosis. Methods A retrospective analysis was performed for the clinical data of 271 patients with liver cirrhosis who attended the Fifth Medical Center of Chinese PLA General Hospital from October 2014 to May 2017, and ARFI elastography was performed for all patients to measure the elasticity of the liver and the spleen. According to the presence or absence of esophageal variceal bleeding, the patients were divided into bleeding group and non-bleeding group, and related indices were compared between the two groups. The receiver operating characteristic (ROC) curve was used to evaluate the value of ARFI elasticity in the diagnosis of esophageal variceal bleeding in liver cirrhosis. The t-test was used for comparison of normally distributed continuous data between two groups, and the Mann-Whitney U test was used for comparison of non-normally distributed continuous data between two groups; the chi-square test was used for comparison of categorical data between two groups. Results The bleeding group had a significantly higher ARFI elasticity of the spleen than the non-bleeding group ［3.89(3.49-4.11) m/s vs 3.46(2.93-3.80) m/s, Z=-4.941, P＜0.001］, and there was no significant difference in the ARFI elasticity of the liver between the bleeding group and the non-bleeding group ［2.08 (1.57-2.74) m/s vs 1.98 (1.49-2.70) m/s, Z=-1.025, P=0.305］. The areas under the ROC curve for ARFI elasticity values of the spleen and the liver were 0.714 and 0.544, respectively, in predicting esophageal variceal bleeding in patients with liver cirrhosis (P=0.002 5). At the cut-off value of 3.71 m/s, the ARFI elasticity of the spleen had a sensitivity of 0.68 and a specificity of 0.69 in predicting esophageal variceal bleeding. Conclusion The ARFI elasticity value of the spleen has a better value than that of the liver in predicting the risk of esophageal variceal bleeding and thus holds promise for clinical application. [ABSTRACT FROM AUTHOR]

Objective: To verify that reducing levels of serum androgen could induce abnormal activation of autophagy by di-n-butyl phthalate (DBP) dming maternal exposure and further investigate the specific mechanism of DBP-induced hypospadias in male offspring. Methods: Pregnant rats were randomly divided into the DBP-treated group and the control group, the former were intragastrically treated with DBP at 750mg per kilogram of the body weight per day on the 14-18 days during gestation while the latter with the equivalent peanut oil. The hypothalamic model of the offspring newborn rats was successfully constructed according to the methods above. The expression level of autophagy markers, including LC3B and Beclinl, in the genital tuber cl e (Gf) was measured by immunohistochemistry (IHC) staining after GT was haivested from male off spring and stored in Formalin. The level of serum testosterone in the rats were measured by radioimmunoassay with blood samples collected after anesthesia in off spring. The expression levels of LC3I, LC 3II and Beclinl were detected by Western blot in primary urethral epithelial cells (PUECs) under treatment with or without dihydrotestosterone. Results: The rate of hypospadias in the DBP-treated group was 42.3%, while no hypospadias was observed in the control group. The autophagy expression was increased apparently in Gf tissues of DBP-treated groups while compared to the control group. The levels of serum testosterone in the DBP group (n= 1 0) were significantly different from those in the control group (n= 1 O)(P<0.05). In vitro studies, the Beclinl and ratio of LC3II/LC3I in the DHT -deficient group were high er than those in the control group. Conclusions: Maternal Exposure to DBP can induce hypospadias, which may be due to DBP-induced autophagy by reducing serum androgen in off-spring. However, the mechanism of this disease still needs further research. [ABSTRACT FROM AUTHOR]

Objective：To probe into the clinical value of laparoendoscopic single-port surgery(LESS) in the treatment of benign gynecological disease. Methods：68 patients who underwent transumbilical single-port laparoscopic surgery at the gynecological department of our hospital between January 2018 and April 2019 were selected as the research group, and another 70 patients with same diseases who underwent conventional multiple ports laparoscopic surgery were chosen as the control group. The indexes such as intraoperative bleeding volume, highest postoperative body temperature, postoperative time for passage of gas by anus, postoperative out of bed activity time, postoperative hospitalized duration, 24 h postoperative VAS, BIS (body image scale) score and incision aesthetic satisfaction CS score, surgical duration and perioperative complications in both groups were compared. Results：The surgeries for patients in both groups were completed smoothly, no intraoperative assisted manipulation was needed and no conversion to laparotomy was done. No severe intraoperative or postoperative complications occurred. There were no significant differences between the intraoperative bleeding volume, highest postoperative body temperature, postoperative time for passage of gas by anus, postoperative activity time, postoperative hospitalized duration in both groups (P＞0.05). However, the differences between surgical duration, 24 h postoperative VAS among patients，body image scale BIS score and incision aesthetic satisfaction CS score were statistically significant (P＜0.05). Conclusions: The transumbilical single-port laparoscopy with conventional instruments do not need special instruments or devices, which conforms to the characteristics of health economics; it is equipped with good cosmetic effect and the advantage of convenient specimen extraction; the application of LESS in the treatment of benign gynecological diseases is safe and feasible. [ABSTRACT FROM AUTHOR]

Objective ：To prepare gype no sides--loaded nanostructured lipid carriers (GPS-NLCs) by hot melting high-pressure homogenization method and investigate its properties and in vitro release. Methods: The effects of stirring power, stirring time, homogeneous pressure and cycle times on the particle size and polydispersity index (PDI) of GPS-NLCs were investigated by single-factor test. The morphology of GPS-NLCs was characterized by transmission electron microscopy (TEM) and dissolution behavior of GPS-NLCs was evaluated in vitro. Results: The optimized particle size of GPS-NLCs was (159 .6士6 .6) nm, PDI was (0 .221 士CX 009) and entrapment efficiency (EE) and drug loading (DL) were (74，30士3 .17)5 and (4 .91 士0 .39)， respectively. As compared with GPS powder and gypenosides-loaded solid lipid nano particles, GPS-NLCs could improve drug dissolution significantly. Conclusion: The particle size distribution of GPS-NLCs prepared by high pressure homogenization method was uniform，the drug entrapment efficiency was high，the dissolution rate of the drug in vitro could be significantly improved with obvious sustained drug release. [ABSTRACT FROM AUTHOR]

Objective To observe and evaluate the clinical efficacy and safety of albumin-bound paclitaxel as first-line treatment for patients with advanced pancreatic cancer in China, and to explore the prognosis-related molecules in pancreatic cancer based on next-generation sequencing (NGS) of tumor tissues. Methods From December 2018 to December 2020, patients with locally advanced or metastatic pancreatic cancer were recruited to accept albumin-bound paclitaxel as first-line treatment in the oncology departments of 24 hospitals in East China. The primary endpoints were overall survival (OS) and treatment related adverse events, and the secondary endpoint was progression-free survival (PFS). Adverse effects were graded using Common Terminology Criteria for Adverse Events 5.0 (CTCAE 5.0). NGS sequencing on the primary or metastatic tissue samples of pancreatic cancer obtained through surgical resection or biopsy was performed. Results This study recruited 229 patients, including 70 patients with locally advanced pancreatic cancer (LAPC) and 159 patients with metastatic pancreatic cancer (mPC). The disease control rate was 79.9% and the objective response rate is 36.3%.The common adverse effects during treatment were anaemia (159 cases), leucopenia (170 cases), neutropenia (169 cases), increased aminotransferases (110 cases), and thrombocytopenia (95 cases), and the incidence of grade 3-4 neutropenia is 12.2% (28/229). The median follow-up time was 21.2 months (95% CI: 18.5-23.1 months). The median PFS (mPFS) was 5.3 months (95% CI: 4.37-4.07 months) and the median OS (mOS) was 11.2 months (95% CI: 9.5-12.9 months). The mPFS of patients with LAPC was 7.4 months (95% CI: 6.6-11.2 months), and their mOS was 15.5 months (95% CI: 12.6-NA months). The mPFS of patients with mPC was 3.9 months (95% CI: 3.4-5.1 months), and their mOS was 9.3 months (95% CI: 8.0-10.8 months). Multivariate Cox regression analysis showed that clinical stage (HR=1.47, 95% CI: 1.06-2.04), primary tumor site (HR=0.64, 95% CI: 0.48-0.86), Eastern Cooperative Oncology Group Performance Status (ECOG PS) score (HR=2.66, 95% CI: 1.53-4.65), and whether to combine radiotherapy (HR=0.65, 95% CI: 0.42-1.00) were independent influencing factors for the PFS of these patients. The primary tumor site (HR=0.68, 95% CI: 0.48-0.95), ECOG score (HR=5.82, 95% CI: 3.14-10.82), and whether to combine radiotherapy (HR=0.58, 95% CI: 0.35-0.96) were independent influencing factors of the OS of these patients. The most frequent gene mutations in these advanced stage pancreatic patients were KRAS (89.66%), TP53 (77.01%), CDKN2A (32.18%), and SMAD4 (21.84%) by NGS of tumor tissues from 87 pancreatic cancer patients with sufficient specimens. Further analysis revealed that mutations in CDKN2B, PTEN, FGF6, and RBBP8 genes were significantly associated with an increased risk of death (P<0.05). Conclusion Albumin-bound paclitaxel as first-line treatment demonstrated feasible anti-tumor efficacy and manageable safety for patients with advanced pancreatic cancer in China. [ABSTRACT FROM AUTHOR]

6082-T6 aluminum alloy additive block was successfully obtained by FSAM technology in this paper， and its corrosion resistance was studied. The results show that the corrosion resistance of the additive is better than that of the base materalby both spal ng corroson rat ng and stat c weghtloss method. The potentodynamc polarization curves and immersion corrosion test also show that the corrosion tendency and corrosion rate of the additive are lower than that of the base material and the corrosion tendency and corrosion rate gradualy decrease from the botom up along the drecton of the addtve heght. After FSAM，the coarse zonal grans of 6082-T6 aluminum alloy are transformed into fine equiaaxial grains，and the amount and distribution of Mg2Si phase are reduced and uniform ，which are the main reasons for the corrosion resistance of the additve body is better than that of the base metal. [ABSTRACT FROM AUTHOR]

Objective To investigate the upstream transcription mechanism of acylglycerol kinase (AGK) in T lymphocyte leukemia, construct recombinant plasmids with different truncated fragments of the AGK gene promoter and detect their transcriptional activity. Methods Public databases such as TIMER2.0, The Human Protein Atlas, and Gene Expression Profiling Interactive Analysis were used to explore the role of AGK in non⁃solid tumors. Using the pGL4. 20⁃pAGK promoter luciferase full⁃length plasmid as a template, PCR was used to amplify truncated fragments of different lengths of the AGK promoter. The amplified fragments were inserted into the pGL4. 20⁃basic vector respectively to construct recombinant plasmids with different truncated fragments of the AGK promoter. After double enzyme digestion and sequence identification were correct, the recombinant plasmid was electroporated and transfected into human T lymphocytic leukemia cell Jurkat, and the dual⁃luciferase reporter gene assay was used to determine the dual luciferase activity of different truncated fragments of the AGK promoter. Results The expression of AGK was up⁃regulation in a variety of tumor types (P<0.05), and the expression level of AGK in leukemia cells was at a higher level. Log⁃rank test showed that the overall survival of patients with high AGK expression was lower than that of patients with low AGK expression (P=0.028）. Three high ⁃scoring binding regions of transcription factor TEAD and AGK were predicted by JASPAR website, and the recombinant plasmids containing different truncated fragments of the AGK gene promoter were successfully constructed. Dual luciferase reporter gene assay showed that the transcriptional activity of AGK promoter was higher in the -540 bp to -80 bp region in T lymphocyte leukemia cells. Conclusions The transcription activity of the AGK gene promoter in the -540 bp to -80 bp region was higher in human T lymphocytic leukemia cells, laying the foundation for further research on the transcription and upstream regulatory mechanisms of AGK in T lymphocyte leukemia. [ABSTRACT FROM AUTHOR]

目的 观察左西孟旦治疗急性冠脉综合征经皮冠状动脉介入治疗（PCI）手术后无复流伴发心衰的临床效 果。方法 选取急性冠脉综合征PCI手术后病变血管无复流伴发心衰患者298例，随机分为观察组和对照组各149 例。两组均冠脉内推注盐酸硝普钠 200 µg+盐酸替罗非班 10 µg/kg，观察组同时微泵静脉滴注左西孟旦 0. 2 µg/（kg·min），持续滴注24 h。记录两组治疗后2周TIMI血流分级、TMPG心肌灌注分级及治疗1年后不良心血管事件 （MACE）发生率，比较其治疗前及治疗后2周血清N末端氨基末端脑钠肽前体（NT-proBNP）水平、心脏超声检查相关 指标［左心室射血分数（LVEF）、心脏指数（CI）、每搏输出量（SV）］。结果 两组TIMI血流分级及TMPG心肌灌注分 级情况比较 P均>0. 05。与治疗前比较，两组治疗后 2周血清 NT-proBNP水平均降低，LVEF、CI、SV均升高，且观察 组变化更明显（P 均<0. 05）。观察组发生再发心绞痛 5 例、再发心肌梗死 3 例、心源性死亡 0 例、心衰再入院 4 例， MACE 发生率为 8. 05%（12/149），对照组分别为 9、5、1、8 例及 15. 44%（23/149），两组 MACE 发生率比较 P<0. 05。 结论 左西孟旦对急性冠脉综合征PCI手术后病变血管无复流且伴发心衰患者的血流灌注影响不大，但能有效改 善患者的心功能，安全性较高。 [ABSTRACT FROM AUTHOR]

Objective: To detect the expression of micro RNA-210 (miR-210) and hypoxia-inducible factor 1α (HIF- 1α) in placenta of pre-eclampsia (PE) patients, and analyze the relationship between them as well as the correlation between the expression and clinicopathological characteristics of PE patients. Methods: 46 pregnant women with PE admitted to our hospital from January 2016 to February 2018 were selected as the study subjects. According to the severity of PE, 21 cases were divided into mild group and 25 cases into severe group. Another 34 pregnant women with normal pregnancy examination and cesarean delivery were selected as the control group. The clinical symptoms of PE patients were analyzed by routine biochemical indicators test. Fluorescent quantitative PCR was used to detect the expression of miR-210 and HIF-1α mRNA in placenta. Immunohistochemical staining was used to detect the expression of HIF -1α in placenta. And the correlation between the expressions of miR-210 and HIF-1α in placenta of PE patients and their relationships with the basic pathological data of patients were analyzed. The receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of miR-210 and HIF-1α mRNA expression levels in PE. Results: Systolic blood pressure (SBP), diastolic blood pressure (DBP) and urinary protein (PRO) levels in PE patients were significantly higher than those in the control group (P＜0.05), and severe PE group were significantly higher than mild PE group (P＜0.05). The levels of miR-210 and HIF-1α in placenta of PE patients were significantly higher than those of control group (P＜0.05), and severe PE group were significantly higher than mild PE group (P＜0.05). The levels of miR-210 and HIF-1α in placenta were correlated with the levels of SBP, DBP, PRO and the severity of PE (P＜0.05). The expressions of miR-210 and HIF-1α in placenta of PE patients were positively correlated (P＜0.05). ROC analysis showed that the cut-off values of miR-210 and HIF-1α mRNA were 1.06 and 1.27, and the sensitivity of PE diagnosis was 80.43% and 78.26%, the specificity was 70.59% and 82.35%. Conclusions: MiR-210 and HIF-1α are highly expressed in the placenta of PE patients, and they are positively correlated with each other, and participate in the occurrence and development of PE disease. [ABSTRACT FROM AUTHOR]

Aiming at the limited usage of hybrid permanent-magnet synchronous motor in servo-control applications, based on its structural features, the flux-weakening performance was analyzed, and the control strategies were summarized and studied. A sensorless control strategy was proposed which applies constant current-frequency ratio control in its low-speed range and uses state-observer as the feedback for rotor position during the high-speed range, the experimental platform based on STM32 ARM Cortex-M4 MCU was set up, the closed-loop control mode can be switched to when 20 percent of the base speed is reached, and the speed can be expanded to 4 times of the base speed, this verified the effectiveness of the control strategy. The results indicate that the hybrid permanent-magnet synchronous motor has excellent fluxweakening performance, and the sensorless control strategy can meet the performance requirements of the servo system with wide speed range on the basis of cost advantages. It expands the application range of the hybrid PMSM driving system. [ABSTRACT FROM AUTHOR]

Topology optimization technology was introduced into the design of nodes in cable-strut structures. Taking several typical nodes in a herringbone ribbed cable dome structure as research objects, the optimization program Altair Solidthinking Inspire was used to carry out static topology optimization under given load cases, where maximization of stiffness under volume constraint and minimization of mass under stress constraint were taken as optimization objectives. Key topology features were further extracted from the optimized results and new models were built by the usage of Evolve, leading to nodes with rational load-bearing behavior and design aesthetic perception. Finite element software ABAQUS was employed to analyze and compare the mechanical property of optimized nodes with initial designed ones in order to comment on the mechanical performance of the new designed nodes. For the nodes which are supposed to directly provide the stiffness，maximization of stiffness under volume constraint should be chosen as the primary optimization objective. For the nodes that serve as connecting components rather than providing stiffness, minimization of mass could be the optimization objective. Finally, additive manufacturing is utilized to realize practical manufacturing of the optimized nodes with complex and irregular geometry. [ABSTRACT FROM AUTHOR]

Groundwater in a many regions is an important support or supplement for ecological stability, the critical depth of recharge of surface vegetation by groundwater is critical to groundwater management and ecological security. Taking the West Liaohe Plain as an example, five results were concluded as follows: (1) The moisture movement structure in vadose zone was innovatively demarcated based on recharge of the vegetation by groundwater, and the mechanism and physical process of recharge of the vegetation by groundwater were described, and the critical depth of recharge of the vegetation by groundwater and related physical concepts were defined. (2) According to the physical mechanism of absorbing the phreatic evaporation by the root system of the vegetation, the phreatic water affected layer which is formed by the rise of the capillary water is the key to critical depth. (3) The rational formula of the soil effective pore size was deduced, and the porosity parameter was calculated based on crystal structure model of soil particles, which dissolved the precise calculation of the maximum height of capillary water. (4) The calculation model for critical depth of recharge of the vegetation by groundwater was built by thickness of the root system. (5) The results were confirmed by massive field surveys. The results of the study were timely applied in the Horqin Grassland. [ABSTRACT FROM AUTHOR]

瘢痕的防治一直是临床的重点和难点, 颗粒脂肪经过加工可得到乳糜化脂肪, 含有基质血管成分、脂肪间充质干 细胞和多种活性因子, 通过抑制病理性瘢痕相关信号通路、提高瘢痕局部氧供给、抗氧化应激损伤、调节免疫和减轻炎症反应等 机制防治增生性瘢痕, 对萎缩性瘢痕也具有良好的治疗效果, 可单一或与其他方法联合使用, 具有简单安全、效果较好、并发症 较少等优点, 是具有广阔应用前景的新型瘢痕防治方法。笔者回顾总结了增生性瘢痕治疗方法的特点, 乳糜化脂肪的主要功能 成分, 防治增生性瘢痕的机制, 制备方法、过程对基质血管成分细胞的影响及防治瘢痕的临床应用作一综述, 以期为乳糜化脂肪 的临床应用和基础研究提供参考。 [ABSTRACT FROM AUTHOR]

Objective To explore the regulatory role of immunoinflammatory mechanism regulated by tiny RNAs (miRNAs) in the pathogenesis of spinal cord injury-induced osteoporosis (CSCI-OP). Methods Thirty patients with CSCI-OP admitted to our hospital from July 2021 to July 2022 were selected as the observation group， fifty patients with senile osteoporosis were selected as the control group， and fifty healthy people were selected as the normal control group. The three groups were compared in terms of hormone indexes， inflammatory factors， miR⁃ NAs expression and bioinformatics analysis. The main factors affecting the pathogenesis of CSCI-OP patients were detected. Results Compared with the spinal cord injury group， the T and TNF-α levels were increased， and the E2， IL-6 and BLAP levels were decreased in the senile osteoporosis group (P < 0.05)， but there was no significant difference in 25-OHD and PTH levels between the two groups (P > 0.05). Univariate analysis showed that the inci⁃ dence of CSCI-OP was mainly related to age， sex， duration of spinal cord injury and diabetes mellitus (P < 0.05). Compared with the normal control group， the expression of IL-6 miRNA and BLAP miRNA in the control group and observation group was increased and the expression of TNF-α miRNA was decreased (P < 0.05). Conclusion MiRNAs can be effectively regulated in CSCI-OP. It can significantly slow down the occurrence and development of CSCI-OP through targeted immune inflammatory mechanisms. [ABSTRACT FROM AUTHOR]

Bronchial asthma is a worldwide chronic airway inflammatory respiratory disease, in recent years, with the increase of air pollution and the increasing number of smokers, the incidence of the disease has increased year by year, which has attracted more and more people’s attention. Bronchial asthma is caused by a variety of factors such as genetic factors, biological factors, psychological factors and other factors, with the deepening of research, great progress has been made in the study of the effect of psychological factors on bronchial asthma, there are obvious personality characteristics and emotional disorders in the patients with bronchial asthma, and the patient’s personality characteristics and emotional disorders will have a certain impact on the patient’s condition and quality of life. In view of this, this article gives a brief account of the personality characteristics, emotional characteristics and its influence on the disease for the clinical reference. [ABSTRACT FROM AUTHOR]

Objective To investigate the clinical effect of transjugular intrahepatic portosystemic shunt(TIPS) alone versus TIPS combined with stomach and esophageal variceal embolization(SEVE) in the treatment of gastroesophageal variceal rebleeding in cirrhotic patients.Methods A total of 142 cirrhotic patients with gastroesophageal variceal bleeding who underwent TIPS or TIPS + SEVE in Department of Gastroenterology,Drum Tower Hospital,from January 2009 to December 2013 were enrolled and divided into TIPS group with 31 patients and TIPS + SEVE group with 111 patients. The two groups were analyzed in terms of intraoperative conditions,follow-up results,and risk factors for postoperative rebleeding. The independent samples t-test was used for comparison of continuous data between groups,the chi-square test was used for comparison of categorical data between groups,and the Mann-Whitney U test was used for comparison of ranked data between groups. The Kaplan-Meier method was used to plot the curves of rebleeding rate,shunt dysfunction rate,and survival rate,and the Log-rank test was used for the comparison of cumulative rebleeding rate,shunt dysfunction rate,and survival rate. The Cox proportional hazards regression model was used for univariate and multivariate analyses. Results All 142 patients completed the surgery successfully. There were no significant differences between the TIPS group and the TIPS + SEVE group in mean follow-up time(34 ± 23 months vs32 ± 21 months,t = 0. 501,P > 0. 05) and incidence rate of hepatic encephalopathy after surgery(12. 9% vs 18. 9%,χ2= 0. 641,P >0. 05). There were no significant differences between the TIPS group and the TIPS + SEVE group in 1-,2-,3-,and 5-year cumulative rebleeding rates during follow-up(χ2= 1. 511,P > 0. 05),while there were significant differences between the two groups in 1-,2-,3-,and 5-year cumulative shunt dysfunction rates(13%/21%/34%/34% vs 7%/12%/13%/13%,χ2= 4. 087,P < 0. 05).There were no significant differences between the two groups in 1-,2-,3-,and 5-year cumulative survival rates during follow-up(χ2= 0. 471,P > 0. 05). Preoperative Child-Pugh class(hazard ratio [HR]= 0. 385,95% confidence interval [CI]: 0. 165-0. 898,P <0. 05),stent type(HR = 0. 429,95% CI: 0. 240-0. 766,P < 0. 05),and postoperative shunt dysfunction(HR = 5. 840,95% CI: 2. 566-13. 294,P < 0. 05) were influencing factors for postoperative rebleeding in cirrhotic patients with gastroesophageal variceal bleeding. The multivariate analysis showed that bare stent(relative risk [RR]= 0. 527,95% CI: 0. 290-0. 954,P < 0. 05) and postoperative shunt dysfunction(RR = 4. 436,95% CI: 1. 899-10. 360,P < 0. 05) were independent risk factors for postoperative rebleeding in cirrhotic patients with gastroesophageal variceal bleeding. Conclusion Compared with TIPS alone,TIPS + SEVE can reduce the incidence rate of postoperative shunt dysfunction in cirrhotic patients with gastroesophageal variceal bleeding,but it cannot reduce postoperative rebleeding rate or increase survival rate. Bare stent and shunt dysfunction are independent risk factors for postoperative rebleeding in cirrhotic patients with gastroesophageal variceal bleeding. [ABSTRACT FROM AUTHOR]

BACKGROUND: Immune eye diseases such as hyperthyroidism exophthalmos and uveitis seriously endanger the eye health of patients, which are common and difficult eye diseases. Current treatments for these diseases include oral administration of hormones and immunosuppressive agents, with poor efficacy, recurrent attacks and poor prognosis. Meanwhile, these treatments can induce systemic adverse reactions. Lymphocytes are directly or indirectly involved in these diseases. Therefore, we try to make papua eye patch carrying immunosuppressant, and deliver the drug through the topical use. Cyclosporin A microemulsion targeting lymphocytes can treat or control palpebral lymph nodes involved in the immune eye diseases. It is a topical method rather than the systemic medication, which is targeted and has small doses of drugs. If possible, this treatment can effectively treat immune eye diseases and avoid systemic drug adverse reactions and long-term adverse reactions induced by original drugs. OBJECTIVE: To study the preparation of cyclosporin A microemulsion papua cream eye patch, and its transdermal absorption characteristics in vitro. METHODS: Cyclosporine A microemulsion was fully mixed with water-soluble polymer materials at a ratio of 1 mg:1 mL, including sodium polyacrylate, polyvinyl alcohol, polyvinylpyrrolidone, gelatin, peach gum, sodium carboxymethylcellulose, hydroxypropylcellulose, and then coated onto the non-woven fabric to prepare Babu cream. Permeability of the Babu cream on the abdominal skin of ICR mice was determined by Franz diffusion cell method. High-performance liquid chromatography was used to detect the concentration of cyclosporine A, and skin irritation and anaphylaxis were also measured. RESULTS AND CONCLUSION: Cyclosporin A microemulsion papua cream eye patch was successfully prepared with appropriate viscosity, good permeability, good permeability, comfortable application, no skin irritation and allergic reaction. The content of cyclosporine A was 10 mg/tablet, and the concentration was 1 g/L. The concentration of cyclosporine A microemulsion increased with the increase of time, and it had good transdermal effect. This study proved that it is feasible to prepare cyclosporine A microemulsion into papua patch. It has good performance in skin permeability, adhesion and skin comfort. [ABSTRACT FROM AUTHOR]