骨髓间充质干细胞对 COPD-OSA 重叠综合征大鼠肺血管内皮损伤的修复作用观察.

Objective To investigate the repair effect of bone marrow mesenchymal stem cells (BMSCs) on pulmonary vascular endothelial injury in rats with chronic obstructive pulmonary disease (COPD) -obstructive sleep apnea (OSA) overlap syndrome and to explore its possible mechanism. Methods BMSCs were isolated from femur and tibial bone marrow of SPF-grade female SD rats, and then were cultured and identified. Sixteen female SD rats with SPF grade were selected to prepare the RAT models of COPD-OSA overlap syndrome by smoking combined with intermittent hypoxia. The rats were randomly divided into the observation group and control group, with 8 rats in each group. The rats in the observation group were injected with 2 × 106 BMSCs per time, once a week, for 4 times in total, and the rats in the control group were injected with normal saline of the same volume. One week after treatment, the rats in the two groups were sacrificed, the lung tissues were taken for paraffin section for HE staining and MASSON staining to observe the pathological changes of the lung tissues and pulmonary arteriole, and the apoptosis of vascular endothelial cells in the lung tissues was observed by the triple qualitative method of DAPI-CD34-TUNEL,the expression levels of endothelin 1 (ET-1), endothelial nitric oxide synthase (e NOS), vascular endothelial growth factor (VEGF) and stromal cell derived factor 1 (SDF-1) were detected by ELISA. Results Compared with the control group, the pathological changes of the lung tissues in the observation group were milder, such as emphysema, interstitial lymphocyte infiltration, neutrophil infiltration, bronchial wall lymphocyte hyperplasia, bronchiolar wall smooth muscle hyperplasia and goblet cell hyperplasia; the changes of pulmonary arterioles myosization, outer membrane collagen fiber deposition, abnormal vascular wall structure, perivascular inflammation, and pulmonary interstitial thickening were relatively mild. The apoptosis rates of pulmonary vascular endothelial cells in the observation group and the control group were 2. 3552% ± 0. 3923% and 1. 3339% ± 0. 1553%,respectively,with statistically significant difference (both P ＜ 0. 01). The content of ET-1 and VEGF in lung tissue homogenization of the observation group was lower than that of the control group, and the content of e NOS was higher than that of the control group, with statistically significant difference (all P ＜ 0. 05); the SDF-1α content in lung tissue homogenization in the observation group was lower than that in the control group, but no statistically significant difference was found between these two groups (P ＞ 0. 05). Conclusions BMSCs can improve the vascular endothelial injury and dysfunction in pulmonary tissues of rats with COPD-OSA overlap syndrome, reduce the apoptosis of pulmonary vascular endothelial cells, and improve the remodeling of pulmonary tissues and small pulmonary vessels to some extent. [ABSTRACT FROM AUTHOR]