中心切割反相&反相二维液相色谱-串联质谱法 测定烟叶潜香类糖苷.

A method for glycosides detection in tobacco leaves by heart-cutting Rp/Rp two- dimensional liquid chromatography-tandem mass spectrometry was established. Three glycosides were determined, including 3-oxo-7, 8-dihydro-α-ionol-,β-D-glucopyranoside, 3-oxo-α-ionol-, β-D-glucopyranoside and 3-hydroxy-, β-dihydro-damascone-pi-β-glucopyranoside. Only extraction and filtration were required in sample pretreatment. The RSDs of intra-day and inter-day were in the range of 1. 0 % -3. 2 % and 2. 8 % -8. 4 %, respectively, which showed good reproducibility. Since sample pretreatment was very simple, the method was quite suitable for daily detection. Different types of tobacco were determined, including Virginian-type tobacco, Burley tobacco, oriental tobacco and sun-cured tobacco. The results revealed that glycosides influecured and fresh Virginian-type tobacco were significantly higher than other types, which agreed well with previous reported articles. 157 kinds of Virginian-type tobacco samples from different regions were monitored and investigated. Among the factors, which influenced contents of glucosides in Virginian-type tobacco, sugar was no longer the major one, catabolite of carotenoids (C13-isoprenol glucosides) and temperature were also considered an important factor in our study. [ABSTRACT FROM AUTHOR]