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In order to transfer and reduce the drought risk in the Weibei region, and ensure economic income and food production security for farmers, meteorological data, spring maize yield and growth period from 1990 to 2020 for 23 counties of the Weibei region were used in this study. The model between drought index and yield reduction rate was established in the specific growth period. Then the spring maize drought weather index insurance product in the Weibei region was designed and the pricing research was carried out. Therefore, the insurance rate was adjusted by using the drought risk assessment results in case to reduce the basis risk. The results showed that: (1) the seedling-milk ripe stage was the key period of water demand for spring maize in the Weibei region. And there was a significant linear relationship between drought index (I) and yield reduction rate (D) during this growth period: D=0.383I-3.959 (P<0.05). (2) The precipitation during the seedling-milk ripe period decreased from northwest to southeast while the drought risk increased from northwest to southeast. And the drought risk in southeast was higher than that in the northwest. (3) Based on the drought yield reduction rate model and the insurance regulations, 40% of the drought index was taken as the compensation trigger value. Then the different drought index compensation standards were determined, and the adjusted insurance rates were between 6.9%-8.9% and the premiums were 375-480 Yuan per hectare based on the drought risk assessment results. This study can provide a scientific basis for the Weibei spring maize weather index insurance products designation, premium pricing, and basis risk effectively reduction. [ABSTRACT FROM AUTHOR]

The one-dimensional nuclear magnetic resonance (NMR) method cannot distinguish the relaxation signals of oil and water during flooding, so longitudinal relaxation-transverse relaxation (T1 -T2 ) two-dimensional NMR method was used to distinguish the type and distribution of fluids in the cores. The enhanced oil recovery (EOR) of the SP flooding system consisting of polymer BRH-325 and surfactant GSS-A was studied by flooding experiments at 65, 70, 75 ℃, respectively. T1 -T2 two-dimensional NMR method was used to visually study the flooding law and distribution characteristics of oil and water in the cores. And the experimental measurement results were compared with the NMR measurement results. The results indicate that the SP flooding has the most significant effect on EOR at 70 ℃ . Therefore, T1 -T2 two-dimensional NMR can easily and clearly distinguish the relaxation signals of oil and water during flooding. [ABSTRACT FROM AUTHOR]

Traditional methods have various problems such as poor robustness and low accuracy in the remaining service life of rolling bearings. In recent years, the development of deep learning has provided new ideas for solving these problems. In order to further improve the accuracy of predicting bearing life, a rolling bearing life prediction method based on ConvNeXt network, stacked bidirectional long shortterm memory network (SBiLSTM) and self-attention mechanism (Self-Attention) was proposed. Firstly, continuous wavelet transform (CWT) was used to construct the time-frequency map of the vibration signal, in order to better capture the time-domain and frequency-domain characteristics of the signal. Then, the obtained time-frequency map was input into the constructed ConvNeXt network, and key features of the time-frequency map were extracted through operations such as convolution, pooling, and layer normalization. Finally, the extracted features were input into the SBiLSTM-Self-Attention module for further extraction of temporal information and feature weight allocation. The PHM2012 challenge data set was used for experimental verification. The root means square error (RMSE) and mean absolute error (MAE) of the proposed method were experimentally analyzed. The results show that, comparing with existing technical methods, the average RMSE of this method is 0. 031. Comparing with the other three comparison methods, convolutional neural network (CNN), deep residual networkbidirectional gated recurrent unit ( DRN-BiGRU) and deep convolutional neural network-self attention-bidirectional gated recurrent unit (DCNN-Self Attention-BiGRU), its average RMSE values respectively decrease by 79%, 74% and 55%, the average MAE values respectively decrease by 78%, 73% and 53%. This method has achieved good performance in predicting the remaining life of rolling bearings. [ABSTRACT FROM AUTHOR]

The evolution of rock pore structure during the hydraulic fracturing process is highly complex, and quantifying changes in pore volume is crucial for understanding the mechanism of hydraulic fracturing. The hydraulic fracturing experiments were conducted on rock cores using a self-designed experimental setup, and NMR(nuclear magnetic resonance) technology was employed to quantitatively characterize the evolution of pore structure throughout the hydraulic fracturing process. The results show as follows. The pressure response of rocks during hydraulic fracturing is influenced by the injection rate and is correlated with the initial permeability of the rock core. Lower initial permeability results in a smaller response in pressure accumulation to injection rate, whereas higher initial permeability cores exhibit a significant response to injection rate variation. Complete pore volume evolution during the hydraulic fracturing process was obtained through NMR. Changes in macropore volume dominate the variation in rock pore volume, with a significant increase observed with increasing injection rate. Moreover, this effect becomes more pronounced with a higher initial permeability of the rock core. The fluid injected during the hydraulic fracturing process not only creates hydraulic fractures but also causes significant changes in rock pore volume, ultimately enhancing the initial permeability of the rock. The injected fluid causes changes in rock porosity and permeability. What is traditionally considered ineffective fluid leak-off in conventional hydraulic fracturing plays a crucial role in reservoir stimulation. The findings provide novel insights for further enhancing oil and gas recovery. [ABSTRACT FROM AUTHOR]

BACKGROUND: With the continuous improvement and progress of artificial intelligence technology in the treatment of spinal deformity, a large number of studies have been invested in this field, but the main research status, hot spots and development trends are still unclear. OBJECTIVE: To visually analyze the literature related to artificial intelligence in the field of spinal deformities by using bibliometrics, identify the research hotspots and shortcomings in this field, and provide references for future research. METHODS: The core database of Web of Science was used to search the articles related to artificial intelligence in the field of spinal deformities published from inception to 2023. The data were visually analyzed by Citespace 5.6.R5 and VOSviewer 1.6.19. RESULTS AND CONCLUSION: (1) A total of 165 papers were included, and the number of papers published in this field showed a fluctuating upward trend. The author with the largest number of articles is Lafage V, and the country with the largest number of articles is China. (2) Keyword analysis results show that adolescent scoliosis, deep learning, classification, precision and robot are the main keywords. (3) The in-depth analysis results of co-cited and highly cited articles show that artificial intelligence has three hotspots in the field of spinal deformities, including the use of U-shaped architecture (a mature mode of deep learning convolutional neural networks) to automatically measure imaging parameters (Cobb angle and accurate segmentation of paraspinal muscles), multiview correlation network architecture (i.e., spine curvature assessment framework), and robot-guided spinal surgery. (4) In the field of artificial intelligence treatment of spinal malformations, the mechanism research such as genomics is very weak. In the future, unsupervised hierarchical clustering and other machine learning techniques can be used to study the basic mechanism of susceptibility genes in the field of spinal deformities by genome-wide association analysis and other genomics research methods. [ABSTRACT FROM AUTHOR]

创伤后成长是指个体在经历创伤性应激事件后产生的积极心理变化, 对个体的成长与发展具有正向导向作 用。护生是创伤事件发生率较高的群体之一。对护生创伤后成长的测评工具与方法、研究现状与影响因素以及提高 护生创伤后成长的干预策略进行综述, 以期提高护理管理者对护生创伤后成长的重视, 为制定合理、有效的护生创 伤后成长培养模式提供参考依据。. [ABSTRACT FROM AUTHOR]

Objective To summarize the existing animal models of cholelithiasis, and to explore a pathological model that can better reflect the characteristics of clinical syndromes of traditional Chinese and western medicine and meet the needs of the development of clinical and basic research of traditional Chinese medicine. Methods The animal models for experimental research on cholelithiasis at home and abroad were collected and sorted out by searching CNKI, Wanfang, VIP, SinoMed, PubMed and other databases. The animal types, modeling methods, modeling cycles, detection indicators and positive drugs of the models were statistically analyzed. Results Among the 128 articles included, the animal types of cholelithiasis models were mainly guinea pigs, rabbits and C57BL/6J mice. The most used modeling method is high-fat diet, and the feeding cycle takes eight weeks to complete. High-frequency detection indicators were stone formation rate, total cholesterol, phospholipids, total bilirubin, total bile acid, etc. The commonly used intervention methods are traditional Chinese medicine compound, western medicine and single Chinese medicine or extract. Ursodeoxycholic acid (UDCA) is mainly used for western medicine intervention, whereas the intervention of traditional Chinese medicine mainly includes traditional Chinese medicine compound, acupuncture, exercise, diet and other methods. The positive control drug in the experiment was mostly UDCA. Conclusion With the continuous improvement and development of cholelithiasis animal model, there are many kinds of modeling methods to simulate the clinical manifestations of cholelithiasis in Chinese and western medicine, but there are also some limitations. This paper aims to provide reference for the selection, application and improvement of cholelithiasis model through data mining and characteristic analysis. [ABSTRACT FROM AUTHOR]

Autophagy is a highly conserved cellular degradation pathway that degrades lipid droplets through a process called “lipophagy”. Lipophagy can selectively recognize lipid substances and degrade them, promoting β oxidation and thereby maintaining the balance of intracellular lipid metabolism. The liver regulates lipid droplet metabolism through lipophagy signaling pathways or key molecules, thereby alleviating hepatic steatosis and improving nonalcoholic fatty liver disease (NAFLD) . This article reviews the latest advances in the degradation of hepatic lipid droplets through the three autophagic pathways of macroautophagy, molecular chaperone-mediated autophagy, and microautophagy. The major signaling pathways of AMPK/mTORULK1, ATGL-SIRT1, FGF21-JMJD3, and Akt are involved in the regulation of the lipophagy process and help to maintain the homeostasis of lipid metabolism in the liver, so as to provide new ideas for clinical prevention and treatment of NAFLD. [ABSTRACT FROM AUTHOR]

Objective To explore syndrome differentiation of traditional Chinese medicine (TCM) and medication rules of functional dyspepsia, and to provide reference for modern TCM clinical treatment. Methods The clinical research literature on the treatment of functional dyspepsia with TCM were searched from CNKI, Wanfang, VIP, PubMed and Web of Science databases, TCM compounds were collected and database was established. Excel 2019, Clementine 12.0 and SPSS 21.0 were used for frequency analysis of TCM syndrome types, drug frequency, efficacy classifications, properties, tastes, and meridian tropisms of functional dyspepsia, and association rule analysis, factor analysis and cluster analysis were performed on high-frequency drugs. Results A total of 428 articles were included, and 442 prescriptions were extracted, involving 225 Chinese medicines and 24 high-frequency drugs, among which Codonopsis Radix, Poria, Citri Reticulatae Pericarpium and Bupleuri Radix were most frequently used. Tonic deficiency drugs and regulating qi drugs were the most common efficacy categories. The property was mainly warm, the tastes were mainly bitter, pungent and sweet, most of which belonged to spleen, lung and stomach meridians. Twenty drug combinations were obtained by association rule analysis, nine common factors were extracted by factor analysis, and drugs could be divided into four categories by cluster analysis. Functional dyspepsia with the liver-stomach disharmony is the most common syndrome. Twelve drug combinations were obtained by association rule analysis, three common factors were extracted by factor analysis, and drugs could be divided into two categories by cluster analysis. Conclusion The clinical treatment of functional dyspepsia in TCM is mainly to replenish qi and invigorate spleen, dredge liver and promote qi, replenish deficiency with sweet and warm drugs, disperse pungent and descend bitter, which were compatible with drugs for resolving dampness, relieving external symptoms and clearing heat according to syndrome differentiation. [ABSTRACT FROM AUTHOR]

Objective To verify the performance and explore the clinical application value of a multi-modal pulmonary nodule diagnosis model combined with metabolic fingerprints, protein biomarker CEA and Image-AI via random forest (MPI-RF). Methods • This study enrolled 289 patients with pulmonary nodules who were admitted to the Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine and were detected by low-dose helical computed tomography (LDCT). The patients were divided into malignant nodule group (n=197) and benign nodule group (n=92) based on postoperative pathological results, and the basic information of the two groups was collected and compared. Electrochemiluminescence was used to detect the preoperative serum CEA levels of the patients in the two groups, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used to detect the serum metabolic fingerprints, and the CT image artificial intelligence model Image-AI was used to calculate the image scores. CEA data, serum metabolic fingerprints data and image scores were integrated and input into MPI-RF to calculate the malignant probability score of each patient. The receiver operator characteristic curve (ROC curve) and area under the curve (AUC) were used to evaluate the performance of different models, and the DeLong test was used for comparative analysis, including the diagnostic performance of MPI-RF in different types (solid nodule, pure ground-glass nodule and part-solid nodule) and sizes (diameter<8 mm and diameter 8 mm) of pulmonary nodules, the diagnostic performance comparison of MPI-RF with Mayo Clinic model, veterans administration (VA) model and Brock model, and the diagnostic performance comparison of MPI-RF with lung imaging reporting and data system (Lung-RADS) in benign and malignant nodules. Results • MPI-RF had good diagnostic performance in the differentiation of benign and malignant pulmonary nodules (AUC=0.887, 95% CI 0.848-0.925, sensitivity 81.22%, specificity 83.70%). Among them, the AUC of MPI-RF for solid nodules was 0.877 (95% CI 0.820-0.934), for part-solid nodules was 0.858 (95% CI 0.771-0.946), and for pure ground-glass nodules was 0.978 (95% CI 0.923-1.000). The AUC of MPI- RF was 0.840 (95% CI 0.716-0.963) for nodules within 8 mm diameter and 0.891 (95% CI 0.849-0.933) for nodules larger than 8 mm diameter. Compared with the existing models, the diagnostic performance of MPI-RF was better than that of Mayo Clinic model, VA model and Brock model (all P=0.000). Compared with Lung-RADS, MPI-RF had better diagnostic performance in the total samples and different types of nodules (all P=0.000). Conclusion • MPI-RF is a model for the differential diagnosis of benign and malignant pulmonary nodules with excellent performance, and has potential clinical application value. [ABSTRACT FROM AUTHOR]

Objective: To investigate the evaluation of left ventricular longitudinal systolic function in patients with thyroid dysfunction by three-dimensional speckle tracking imaging (3D-STI) and its relationship with cardiac function indexes and serum myocardial injury markers. Methods: 120 patients with abnormal thyroid function (study group) and 80 patients with normal thyroid function (control group) were selected, the parameters of global circumferential strain (GCS), global longitudinal strain (GLS), left ventricular systolic global area strain (GAS), left ventricular ejection fraction (LVEF), left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic diameter (LVEDS) and left ventricular end-diastolic diameter (LVEDD) were compared between two groups, the levels of serum troponin 1 (cTn1), creatine kinase (CK) and CK isoenzyme (CK-MB) were detected in two groups. The correlation between 3D-STI parameters and conventional ultrasound parameters and the levels of serum cTn1, CK and CK-MB was analyzed by Pearson correlation analysis. Results: There was no significant difference in LVEDD, LVEDS, LVEDV and LVEF between study group and control group (P>0.05), the absolute values of GAS, GLS and GCS in study group were lower than those in control group, The levels of cTn1, CK and CK-MB were higher than those in control group (P<0.05). The absolute values of GAS, GLS and GCS in study group were negatively correlated with the levels of serum cTn1, CK and CK-MB (P<0.05), but not with LVEDD, LVEDS, LVEDV and LVEF (P>0.05). Conclusion: 3D-STI parameters GAS, GLS and GCS are decrease in patients with thyroid dysfunction, and the levels of serum cTn1, CK and CK-MB are increase, 3D-STI parameters can effectively assess the longitudinal function of the left ventricular contraction in patients with abnormal thyroid function. [ABSTRACT FROM AUTHOR]

AIM： To investigate the molecular mechanism of long noncoding RNA (lncRNA) SNHG1 in regulating ferroptosis to alleviate inflammation in CHME-5 human microglia induced by HIV-1 gp120 V3 loop. METHODS： CHME-5 human microglia were cultured in vitro, and were divided into 7 groups： blank group, random peptide group，gp120 V3 loop group (HIV-1 gp120 group), HIV-1 gp120+shCon group, HIV-1 gp120+SNHG1 sh2 group, HIV-1 gp120+SNHG1 sh2+ferrostatin-1(Fer-1； ferroptosis inhibitor) group, and HIV-1 gp120+SNHG1 sh2+EX527(Sirt1 inhibitor) group. Normal CHME-5 cells were treated with random peptide or gp120 V3 loop for 24 h. After pretreatment of SNHG1 sh2 cells with inhibitors for 2 h, the cells were then treated with gp120 V3 loop for 24 h. The levels of inflammatory cytokines in the cell supernatants were detected by ELISA. Western blot was used to detect the protein expression levels of solute carrier family 7 member 11(SLC7A11), glutathione peroxidase 4(GPX4), Sirt1 and p53. Microplate reader was used to detect the levels of intracellular ferrous ion (Fe2+ ) and malondialdehyde (MDA). RESULTS：(1) The results of ELISA showed that the expression levels of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and IL-1β in HIV-1 gp120 group were significantly higher than those in blank group (P<0. 05). Compared with HIV-1 gp120 group, the expression levels of inflammatory cytokines in HIV-1 gp120+SNHG1 sh2 group were significantly decreased (P<0. 05). Compared with HIV-1 gp120+SNHG1 sh2 group, the expression levels of inflammatory factors in HIV-1 gp120+SNHG1 sh2+Fer-1 were significantly decreased (P<0. 05), but those in HIV-1 gp120+SNHG1 sh2+EX527 group were significantly increased (P<0. 01). (2) The results of Western blot showed that compared with blank group, the expression levels of ferroptosis-related proteins SLC7A11 and GPX4 in HIV-1 gp120 group were significantly down-regulated (P<0. 01). Compared with HIV-1 gp120 group, the expression levels of SLC7A11 and GPX4 in HIV-1 gp120+SNHG1 sh2 group were significantly up-regulated (P<0. 01). Compared with HIV-1 gp120+SNHG1 sh2 group, the expression levels of SLC7A11 and GPX4 in HIV-1 gp120+SNHG1 sh2+Fer-1 group were significantly up-regulated (P<0. 05), but the expression levels of SLC7A11 and GPX4 in HIV-1 gp120+SNHG1 sh2+EX527 group were significantly down-regulated (P<0. 01), and the expression level of p53 was significantly up-regulated (P<0. 05). (3) Compared with blank group, the levels of Fe2+ and MDA in HIV-1 gp120 group were significantly increased (P<0. 05). Compared with HIV-1 gp120 group, the levels of Fe2+ and MDA in HIV-1 gp120+SNHG1 sh2 group were significantly decreased (P<0. 01). Compared with HIV-1 gp120+SNHG1 sh2 group, Fe2+ and MDA in HIV-1 gp120+SNHG1 sh2+Fer-1 group were significantly decreased (P<0. 05), but those in HIV-1 gp120+SNHG1 sh2+EX527 group was significantly increased (P<0. 05). CONCLUSION： Knockdown of SNHG1 can attenuate the inflammation in microglia induced by HIV-1 gp120 V3 loop, which may be achieved by regulating ferroptosis-related signaling molecules through the Sirt1/p53 signaling pathway. [ABSTRACT FROM AUTHOR]

Objective: To discuss the effect of forkhead box O1 (FOXO1) gene on the autophagy and apoptosis of the vascular smooth muscle cells in abdominal aortic aneurysm (AAA), and to clarify its possible mechanism. Methods: The aneurysm tissue of nineteen AAA patients (AAA group) and adjacent normal aortic tissue of nineteen AAA patients (control group) were collected. Real-time fluorenscence quantitative PCR (RT-qPCR) method was used to detect the expression level of FOXO1 mRNA in aneurysm tissue of the subjects in two groups; transmission electron microscope was used to observe the autophagolysosome formation in aneurysm tissue of the subjects in two groups; Western blotting method was used to detect the expression levels of FOXO1 and autophagy-related proteins B cell lymphoma-2 (Bcl-2) -binding protein (Beclin1), microtubule-associated protein 1 light chain 3α (LC3), and P62 proteins in aneurysm tissue of the subjects in two groups. The human aortic vascular smooth muscle cells (hVSMCs) were cultured in vitro and infected with FOXO1 siRNA (si-FOXO1) and its negative control (si-NC) lentivirus, then treated with 10 μmol·L-1 angiotensin Ⅱ (Ang Ⅱ ) combined with autophagy activator rapamycin (Rap) . The cells were divided into blank control group, Ang Ⅱ group, Ang Ⅱ + si-NC group，Ang Ⅱ + si-FOXO1 group, Ang Ⅱ + si-NC + Rap group, and Ang Ⅱ + siFOXO1 + Rap group. CCK-8 assay was used to detect the proliferation activities of the cells in various groups; flow cytometry was used to detect the apoptotic rates of the cells in various groups; ELISA method was used to detect the levels of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in the cell supernatant in various groups; RT-qPCR method was used to detect the expression level of FOXO1 mRNA in the cells in various groups; Western blotting method was used to detect the expression levels of FOXO1, Bcl-2, Bcl-2 associated X prorein (Bax), Cleaved-cysteinyl aspartate specific proteinase-3 (Cleaved caspase-3), Beclin1, LC3, and P62 proteins in the cells in various groups. Results: Compared with control group, the expression level of FOXO1 mRNA in aneurysm tissue of the subjects in AAA group was increased (P<0. 05), the number of autophagolysosomes was increased (P<0. 05), the levels of Beclin1 protein and the ratio of LC3 Ⅱ/ LC3 Ⅰ were increased (P<0. 05), and the expression level of P62 protein was decreased (P<0. 05) . Compared with blank control group, the proliferation activity of the hVSMCs in Ang Ⅱ group was decreased (P<0. 05), the apoptotic rate of the cells was increased (P<0. 05), the levels of MMP-2 and MMP-9 in the cell supernatant were increased (P<0. 05), the expression levels of Bax, Cleaved caspase3, and Beclin1 proteins in the cells and the ratio of LC3 Ⅱ/LC3 Ⅰ were increased (P<0. 05), and the expression levels of Bcl-2 and P62 proteins were decreased (P<0. 05) . Compared with Ang Ⅱ + si-NC group, the proliferation activity of the hVSMCs in Ang Ⅱ + si-FOXO1 group was increased (P< 0. 05), the apoptotic rate of the cells was decreased (P<0. 05), the levels of MMP-2 and MMP-9 in the cell supernatant were decreased (P<0. 05), the expression levels of Bax, cleaved caspase-3, and Beclin1 proteins in the cells and the ratio of LC3 Ⅱ/LC3 Ⅰ were decreased (P<0. 05), and the expression levels of Bcl-2 and P62 proteins were increased (P<0. 05). Compared with Ang Ⅱ+ si-FOXO1 group, the apoptotic rate of the hVSMCs in Ang Ⅱ+ si-FOXO1 + Rap group was increased (P<0. 05), the levels of MMP-2 and MMP-9 in the cell supernatant were increased (P<0. 05), the expression level of Beclin1 protein in the cells and the ratio of LC3 Ⅱ/LC3 Ⅰ were decreased (P<0. 05), and the expression level of P62 protein was increased (P<0. 05). Conclusion: Silencing the FOXO1 gene may increase the proliferation activity of the hVSMCs exposed to Ang Ⅱ by reducing the autophagy level and inhibiting the apoptosis, thus participating in the pathogenesis of AAA. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To probe into the effects of different perioperative analgesic regimens on pain degree and rehabilitation efficacy after arthroscopic rotator cuff repair, so as to provide references for optimizing individualized analgesic regimen, promoting early rehabilitation of rotator cuff injury and improving shoulder joint function. METHODS: A total of 150 patients with rotator cuff injury admitted into the hospital from May 2020 to May 2022 were extracted to be divided into three groups via random number table method, with 50 cases in each group. Patients in group A was given ropivacaine injection during surgery, patients in group B was given ropivacaine injection during surgery and oral celecoxib after surgery, patients in group C was given ropivacaine injection during surgery and oral celecoxib during perioperative period. The postoperative rehabilitation, visual analogue scale (VAS) scores at different time points after surgery, inflammatory indicators [interleukin 6 (IL-6), interleukin 10 (IL-10) and prostaglandin E2 (PGE2)], functional rehabilitation [ University of California at Los Angeles ( UCLA) shoulder joint score and American Society for Shoulder and Elbow Surgery (AESE) score], adverse drug reactions of analgesic drugs, rotator cuff recovery at 3 months after surgery were compared among three groups. RESULTS: The hospital stays of group B and group C were respectively (2. 57±0. 56) d and (2. 74±0. 41) d, shorter than (3. 56±0. 34) d of group A; the hospitalization costs of group B and group C were respectively (27. 1±5. 2) thousand yuan and (26. 9±6. 3) thousand yuan, both were shorter than (31. 2±4. 8) thousand yuan of group A; the incidences of system inflammatory reaction syndrome (SIRS) of group B and group C were respectively 14% (7 / 50) and 6% (3 / 50), both were lower than 36% (18 / 50) of group A; at 3 days after surgery, the VAS score of group B and group C was lower than that of group A, all the above differences were statistically significant (P<0. 05). There were no statistical significance in differences in postoperative hospital stay, hospitalization cost, incidence of SIRS, VAS score at 3 days after surgery between group B and group C (P>0. 05). In the comparisons of VAS scores at 7 days, 14 days and 1 month after surgery, group C < group B < group A, with statistically significant differences (P<0. 05). At 7 days, 14 days and 1 month after surgery, the serum IL-6 and PGE2 levels of the three groups decreased, the IL-10 levels increased; in the comparison of serum IL-6 and PGE2 levels, group C < group B < group A, and in the comparison of serum IL-10 levels, group C > group B > group A, with statistically significant differences (P<0. 05). At 14 days, 1 month and 3 months after surgery, the UCLA and ASES scores of the three groups increased, and group C > group B > group A, with statistically significant differences (P<0. 05). The incidences of adverse analgesic drug reactions of group B and group C were respectively 12% (6 / 50) and 18% (9 / 50), the differences were not statistically significant (P > 0. 05). The healing rates of rotator cuff in group A, group B, and group C were respectively 86% (43 / 50), 90% (45 / 50) and 88% (44 / 50), the incidences of re-tear were respectively 14% (7/ 50), 10% (5/ 50) and 8% (4/ 50) in group A, group B and group C, the differences were not statistically significant (P>0. 05). CONCLUSIONS: The analgesia after arthroscopic rotator cuff repair is contributes to shortening hospital stay, accelerating rehabilitation process, relieving postoperative pain, controlling inflammatory reaction caused by stress and improving shoulder joint function, and intraoperative injection of ropivacaine combined with perioperative oral celecoxib is superior in analgesia, anti-inflammation and rehabilitation. [ABSTRACT FROM AUTHOR]

The development of medical imaging technology has generated a massive amount of medical image data, which reflects the internal structural features of the human body. Medical image segmentation technology can improve the efficiency of medical diagnosis, making it an important assistive tool for modern medical diagnosis. However, noise or artifacts that are inevitably present in the imaging process bring great challenges to the segmentation work. In existing segmentation models, single-frame medical image semantic segmentation models do not consider the relationship between image frames, while video semantic segmentation models utilize temporal information but have some limitations in edge extraction. To address these issues, this paper proposes a U-Net-based temporal semantic segmentation model modified by optical flow. This model can extract optical flow information between consecutive frames and perform feature extraction and weight allocation on the current frame and optical flow for correction. Experiments show that the model obtains optimal results on three evaluation metrics, namely Dice similarity, pixel accuracy and cross-merge ratio, on different types of datasets, namely Drosophila electron micrographs, combined healthy abdominal organ segmentation and coronary angiogram, which validate the effectiveness and generalization of the proposed model. [ABSTRACT FROM AUTHOR]

Objective: To study the effect of Long non-coding RNA (LncRNA) small nucleolar RNA host gene 12 (SNHG12) regulating miR-138-5p/hypoxia inducible factor-1 (HIF-1α) axis on improving the damage of hypoxia/reoxygenation (H/R) human vascular endothelial cells. Methods: Human umbilical vein endothelial cells (HUVECs) were cultured in vitro and randomly divided into control group, H/R model group, H/R+LncRNA SNHG12 overexpression group, H/R+miR-138-5p mimics group, H/R+co-transfection group and H/R+co-transfection negative control group, each transfection group was transfected separately, and except for control group, the remaining groups were given hypoxia for 5 hours and then reoxygenated for 1 hour to induce the cell models, and then the cell viability of each group was detected by CCK-8 experiment; the cell apoptosis in each group was detected by flow cytometry experiment, and the apoptosis rate of each group was compared; the levels of reactive oxygen species (ROS), lactate dehydrogenase (LDH) and inflammatory factors IL-6, IL-17 and IL-18 in each group were measured by the kit; the expressions of miR-138-5p and HIF-1α mRNA in cells of each group were measured by real-time quantitative PCR (qRT-PCR) experiment; the expressions of apoptotic proteins caspase-9, Bcl-2-associated X protein (Bax) and HIF-1α in each group were evaluated by Western blot. Results: Compared with control group, the apoptosis rate, cellular ROS, LDH, IL-6, IL-17 and IL-18 levels, cellular HIF-1α mRNA and protein levels, cellular caspase-9, Bax and HIF-1α protein levels were increased in H/R model group (P<0.05), the cell viability and miR-138-5p level were decreased (P<0.05). Compared with H/R model group and H/R+co-transfection group, the cell viability, cell HIF-1α mRNA and protein levels were increased in H/R+LncRNA SNHG12 overexpression group (P<0.05), the apoptosis rate，cellular ROS, LDH, IL-6, IL-17 and IL-18 levels，cellular caspase-9 and Bax protein levels, and miR-138-5p level were decreased (P<0.05); the cell viability，cellular HIF-1α mRNA and protein levels were decreased in H/R+miR-138-5p mimics group (P<0.05), the apoptosis rate, cellular ROS, LDH, IL-6, IL-17 and IL-18 levels，cellular caspase-9 and Bax protein levels were increased (P<0.05). Compared with H/R model group, there was no significant difference in cell index levels between the H/R+co-transfection negative control group and the H/R+co-transfection group (P>0.05). Conclusion: LncRNA SNHG12 can upregulate HIF-1α expression by downregulating miR-138-5p expression, inhibit H/R-induced inflammation and oxidative stress in HUVECs, and reduce cell damage and apoptosis. [ABSTRACT FROM AUTHOR]

In view of the differences in party positions and values, the changes in the situation on the Korean Peninsula and the influence of the US's Indo-Pacific strategy, the Moon Jae-in's administration and the Yoon Suk Yeol's administration's policies towards Japan are significantly different. During the reign of Moon Jae-in's administration, the diplomatic policies of ROK towards Japan were more positive in the early stage. But there was a significant change in the middle and late stages and the government took a tough stance on bilateral historical issues. ROK chose to confront Japan and launched an economic and trade war. There was friction with Japan at security affairs and refused to participate in joint military exercises with Japan. After Yoon Seok Yeol took office, ROK was committed to strengthening the US ROK alliance, restarting ROK Japan "shuttle diplomacy" and making concessions on historical issues for further improvement in bilateral relationship. The two countries resumed economic exchanges and established an "economic security dialogue" mechanism. They also restarted military intelligence cooperation. Compared with the Moon Jae-in administration, the adjustment and shift of the policies towards Japan of Yoon Suk Yeol's administration did not bring progress to the current situation on the Korean Peninsula, but made the situation in Northeast Asia more complex. [ABSTRACT FROM AUTHOR]

Cyclic olefin copolymer (COC) is produced through the copolymerization of ethylene and cyclic olefins, and has been used for the manufacturing of optical devices and diagnostic containers due to its high transparency and excellent water vapor barrier. However, COC with high glass-transition temperature usually needs high cycloolefin insertion in copolymer and has increased brittleness, which has significantly hampered its many end uses. The introduction of α -olefin into ethylene/cycloolefin binary copolymers that have been extensively studied is expected to expand the application of cyclic olefin copolymers. In this paper, we report the terpolymerization of ethylene/norbornene/1-octene and ethylene/dicyclopentadiene/1-octene catalyzed by modified cyclopentadienyl scandium complexes 1−3 activated with [Ph3C] [B (C6F5 ) 4 ] and Ali Bu3 because rare earth catalysts have shown excellent catalytic performance towards olefin polymerization. These rare earth catalysts exhibit high catalytic activities (4. 4×105 ~21. 4×105 g/ (mol (Sc) ·h·bar) ) . The resultant terpolymers show moderate number average molecular weight (Mn=3. 8×104 ~20. 3×104 ) and relatively narrow polydispersity index (PDI=1. 2~2. 4) . The introduction of 1-octene significantly improves the toughness of ethylene/norbornene/1-octene terpolymers, and the P3 sample (Mn 13. 7×104, norbornene 37. 1%, 1-octene 11. 0%) exhibits 2. 0 times higher elongation at break (29. 9% vs. 9. 9%) than the P1 sample (Mn 14. 0×104, NB 42. 6%, OCT 6. 4%) [ABSTRACT FROM AUTHOR]

In order to explain the difference of survival response of plants at different levels in evergreen broad-leaved forest to the Phyllostachys edulis expansion from the perspective of ecological stoichiometry homeostasis, this study used the method of space-time substitution, a typical P. edulis evergreen broad-leaved forest interface including P. edulis forest, P. edulis broad-leaved mixed forest and evergreen broad-leaved forest was selected in Jinggangshan National Nature Reserve, Jiangxi Province. The N and P contents of soil and leaves of P. edulis, broad-leaved trees, shrubs and herbaceous layer plants in each forest were compared and analyzed. The results were as follows:(1)From bamboo forest to evergreen broad-leaved forest, the soil N content and N:P increased, while soil P content decreased; the leaf P contents of tree species(Machilus thunbergii, Alniphyllum fortunei and Daphniphyllum macropodum)decreased and the N:P increased. Unlike the trees, the leaf N and P stoichiometric characteristics of plants both in shrub and herbaceous layers showed no significant change, except the shrub tree Lindera erythrocarpa.(2)The soil N:P was positively correlated with the leaf N:P of trees, negatively correlated with that of shrubs and had no correlation with that of herbaceous plants, respectively.(3)The leaf N and P contents and N:P for P. edulis remained stable. In conclusion, P. edulis expansion changes plant leaf N and P stoichiometric characteristics by altering the soil N and P stoichiometric characteristics. Shrubs and herbaceous plants are less affected, however, it causes the imbalance of plant N and P stoichiometric characteristics of tree layer plants, which may be an important reason for the survival of tree species in evergreen broad-leaved forest. [ABSTRACT FROM AUTHOR]

The experiment was to investigate effect of Eucommia ulmoides Oliv leaves powder on production performance, egg quality, serum biochemical indexes and yolk cholesterol of Hyline brown laying hens. A total of 720 Hyline brown laying hens reared at 327 d of age were randomly divided into five groups, with four replicates in each group and 36 laying hens in each replicate. The hens in control group were fed with basic diet, the hens in experiment groups were fed with 0.5%, 1.0% and 1.5% Eucommia ulmoides Oliv leaves powder in basic diet respectively. The pre-experiment period was 1 w, and formal experiment period was 7 w. The results showed that the laying rate of laying hens in 1.0% group and 1.5% group were significantly higher than that of control group (P<0.05). The eggs yolk color in 1% group was significantly higher than that of control group (P<0.05). The content of TC of laying eggs in control group was significantly higher than that in 1.0% group and 1.5% group (P<0.05). The T-AOC and CAT activity of laying hens in each experiment group were significantly higher than those in control group (P<0.05). The content of egg yolk cholesterol of laying hens in 1.0% group and 1.5% group were significantly lower than that in control group (P<0.05). The experiment indicates that adding Eucommia ulmoides Oliv leaves owder in diet can improve laying rate and egg quality of laying hens, reduce content of serum total cholesterol and egg yolk cholesterol, improve antioxidant capacity, and recommended amount is 1.0%. [ABSTRACT FROM AUTHOR]

AIM: To investigate the relationship between the inflammatory response and ferroptosis induced by human immunodeficiency virus type 1 （HIV-1） gp120 V3 loop in CHME-5 microglia, and to observe the effects of p53 and ferroptosis on inflammatory response and its possible mechanisms. METHODS: Human CHME-5 microglia were cultured in vitro, and were dividied into 5 group: blank group, random peptide group, HIV-1 gp120 V3 loop group, HIV-1 gp120 V3 loop+ferrostatin-1 （Fer-1; an inhibitor of ferroptosis） group and HIV-1 gp120 V3 loop+pifithrin-α （an inhibitor of p53） group. The CHME-5 cells were treated with HIV-1 gp120 V3 loop（ final concentration: 2 mg/L） and random peptide （final concentration: 2 mg/L）for 24 h, or pretreated with Fer-1 （final concentration: 20 μmol/L） or pifithrin-a （final concentration: 10 μmol/L） for 2 h, and then HIV-1 gp120 V3 loop for another 24 h. ELISA was used to detect the expression levels of inflammatory factors in the cell supernatant of each group. Western blot was used to measure the expression levels of ferroptosis-related proteins ［glutathione peroxidase 4 （GPX4）, transferrin receptor-1 （TFR-1） and solute carrier family 7 member 11（ SLC7A11）］ and p53. The level of intracellular ferrous ion（ Fe2+） and the activity of glutathione peroxidase（ GSH-Px） were measured. RESULTS: Compared with blank group and random peptide group, the levels of interleukin-1β（ IL-1β）, tumor necrosis factor-α（ TNF-α） and IL-6 in HIV-1 gp120 V3 loop group were significantly increased （P<0. 01）. Compared with HIV-1 gp120 V3 loop group, the levels of IL-1β, IL-6 and TNF-α were significantly decreased in the HIV-1 gp120 V3 loop+Fer-1 group and HIV-1 gp120 V3 loop+pifithrin-α group （P<0. 01）. Western blot showed that compared with blank group and random peptide group, protein p53 was significantly upregulated（P<0. 01）, ferroptosis-related proteins TFR-1 was up-regulated（ P<0. 01）, SLC7A11 and GPX4 were significantly downregulated（P<0. 01） in HIV-1 gp120 V3 loop group. Compared with HIV-1 gp120 V3 loop group, ferroptosis-related protein TFR-1 was significantly decreased （P<0. 01）, GPX4 and SLC7A11 were significantly up-regulated in HIV-1 gp120 V3 loop+pifithrin-α group（ P<0. 05）. Compared with blank group and random peptide group, the level of Fe2+ was significantly increased（ P< 0. 01）, and the activity of GSH-Px was significantly decreased （P<0. 01） in HIV-1 gp120 V3 loop group. Compared with HIV-1 gp120 V3 loop group, the level of Fe2+ was reduced （P<0. 05） and the activity of GSH-Px was significantly improved in HIV-1 gp120 V3 loop+Fer-1 group and HIV-1 gp120 V3 loop+pifithrin-α group （P<0. 01）. CONCLUSION: Ferroptosis exists in CHME-5 microglial inflammation induced by HIV-1 gp120 V3 loop, and inhibition of ferroptosis can attenuate inflammation. The CHME-5 microglial inflammation induced by HIV-1 gp120 V3 loop is related to the regulation of ferroptosis by p53, and inhibition of p53 can attenuate ferroptosis and inflammatory. [ABSTRACT FROM AUTHOR]

[Purpose/Significance] At present, governments at different levels in China are faced with some problems and challenges in opening up government data such as lagging policy legislation, data unable to meet public demand, insufficient platform service function and integration, imperfect data openness innovation mechanism, and a lack of unified management institutions. The purpose of this paper aims at construction of a stage maturity model to build an evaluation indicator system to measure government data openness maturity, It is important for the local government to understand which level of development their open data is at and which areas need to be improved, in order to comprehensively understand and grasp the actual level of local government data openness in China, which is important for strengthening the construction of sunshine government and e-government, optimizing the process of government data openness, and promoting the innovative development of government data openness. [Method/Process] Based on the idea of the software capability maturity model Capability Maturity Model (CMM), the Chinese government data openness maturity model was constructed, and then a data openness evaluation indicator system was also established on the basis of the maturity model. The government data openness maturity model includes strategic level, data level, technical level, organizational level and application level, and the levels are divided into five categories: initial level, document level, development level, open level and optimization level. The evaluation indicator system consists of 5 primary indicators, 11 secondary indicators and 35 tertiary indicators. The combination weights of the indicators were calculated by using the AHP-entropy weight method. Then, we designed the scoring rules, set the scoring criteria, and scored the four municipalities directly under the central government of China according to the actual situation. [Results/Conclusions] According to the ratings of the four municipalities, Shanghai has a good overall performance, followed by Beijing, Tianjin, and Chongqing. The municipalities directly under the central government are better in terms of related policies launched, but they are lacking in terms of platform function construction, the degree of perfection of local policies and regulations, and the interaction ability with users, which need to be further strengthened. The open government data evaluation indicator system proposed in this paper needs continuous improvement in terms of indicator selection and scoring criteria. In some aspects, the effect of using one indicator alone may not be satisfactory, and the follow-up study involving more elements will summarize more experiences and collect more experts' opinions in the field of government data openness to further improve the government data openness maturity evaluation indicator system. [ABSTRACT FROM AUTHOR]

Objective To conduct a case-control study on precocious puberty as an example to introduce the establishment and design of the electronic Data capture and management platform using Research Electronic data Capture (REDCap) system and support the development of clinical research. Methods Based on the clinical REDCap system, the case-control research project of precocious puberty was created, the case report forms were designed, the user rights were set, and the data quality control rules were formulated. Results We established the electronic data capture and management platform for our research, which had 15 case report forms, to collect the data of the participants, including sociodemographic information, time for rest and activities, diet, exposure to environmental internal-secretion interfering-substances, physical examination and biochemical indicators. We conducted project management by setting up features such as user permissions and workgroups, and added data quality verification rules to control data quality. The data could be exported in various file formats for analysis and sharing. Conclusion The application of REDCap to establish the data capture and management platform of precocious puberty case-control study has promoted the efficient implementation of clinical research, which can be further popularized and applied to clinical researches in other fields. [ABSTRACT FROM AUTHOR]

Objective To validate and evaluate the performance of AVE-562 automatic feces analyzer according to Pharmaceutical Industry Standard of the People’s Republic of China (YY/T 1745-2021). Methods A total of 517 routine feces specimens from outpatients and inpatients at the hospital from November 17 to December 17, 2020, and 100 fecal occult blood positive specimens by manual method from patients with gastric cancer and 100 with colorectal cancer admitted to the hospital from April 6 to 13, 2022 were collected. The results of manual method were regarded as the gold standard. The test performance analysis, including feces physical index, detection limit, detection rate, repeatability, carrying pollution test, coincidence rate, operation speed and the transportation of supplementary feces collector, clinical applicability in patients with gastric cancer and colorectal cancer, were comprehensively evaluated. Results The verification results of feces physical index, detection limit, detection rate, repeatability and carrying pollution test of the AVE-562 automatic feces analyzer were all in line with the evaluation criteria. The coincidence rates of red blood cell (RBC), white blood cell (WBC), fungus and occult blood test were 96. 1%, 90. 5%, 99. 0% and 94. 0%, respectively. The Kappa values of RBC, WBC, fungus and occult blood test were 0. 90, 0. 73, 0. 80, 0. 66, respectively. For patients with gastric and colorectal cancer, the positive coincidence rates of AVE-562 and artificial fecal occult blood results were 96% and 97%, the differences were statistically significant (P<0. 05) concordance rates in fecal occult blood test were observed between AVE-562 and manual methods. The analyzer had good performance in cell recognition and its test indicators were consistent with the manual method. Conclusion The AVE-562 automatic feces analyzer can effectively reduce human resources and improve the efficiency of detection with its characters of high degree of automation, simple operation and high detection rate. It is suitable for clinical application. [ABSTRACT FROM AUTHOR]

Objective: To explore the relationship between rumination and non-suicidal self-injury in adolescents. Methods: Databases such as CNKI, WanFang Data, CBM, PubMed, Web of Science, EMbase, PsycINFO were searched, and the literatures about non-suicidal self-injury and rumination were collected and analyzed by meta-analysis. Results: A total of 15 articles were included, and there were 17 440 subjects. The results of meta-analysis showed that adolescent rumination was positively correlated with non-suicidal self-injury (r=0. 22, Z=12. 82, P<0. 001) . The results of the subgroup analysis showed that different sample sources, cultural backgrounds and measurement tools did not affect the correlation between rumination and non-suicidal self-injury (Q=0. 03, 3. 45, 5. 39, 0. 56, P>0. 05. Conclusion: It suggests that there is a positive correlation between rumination and non-suicidal self-injury in adolescents. [ABSTRACT FROM AUTHOR]

The experiment was to study the effect of dietary complex plant essential oil on growth performance, serum biochemical indexes and intestinal microbiota of broilers. A total of 480 one-day-old broilers were randomly divided into five groups, with six replicates in each group and 16 broilers in each replicate. Broilers in control group were fed with basal diet, and broilers in experimental groups were fed with basal diet supplemented with 35 mg/kg antibiotics (group D1), 50 (group D2), 100 (group D3) and 150 mg/kg (group D4) plant essential oil. The results showed that compared with control group, the average daily gain of broilers in experimental group was significantly increased (P<0.05). The feed to gain ratio of broilers in group D1, group D3 and group D4 was lower than that in control group. Compared with control group, the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum of broilers in group D2, group D3 and group D4 were significantly decreased (P<0.05), and the content of total protein (TP) and urea nitrogen (BUN) were significantly decreased (P<0.05). Compared with control group, the serum total superoxide dismutase (T-SOD) activity of broilers in group D2, group D3 and group D4 was significantly increased (P<0.05). Compared with control group, the total number of bacteria, the number of Escherichia coli and the number of Salmonella in jejunum, ileum, cecum of broilers in experimental group were significantly decreased (P<0.05). The experiment indicates that plant essential oils can significantly improve the growth performance of broilers, reduce the incidence of oxidative stress and liver damage, protect the intestinal health of broilers, and improve the protein utilization rate. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To probe into the effects of serum homocysteine (Hcy) and vascular endothelial growth factor (VEGF) levels on therapeutic effects of Alginic sodium diester tablets in the treatment of patients with transient ischemic attack (TIA). METHODS: Totally 160 patients with TIA admitted into the hospital from Jan. 2020 to Feb. 2021 were selected, all patients were given conventional therapy combined with Alginic sodium diester tablets for 21 d, and 6-month followed up was conducted, the therapeutic effect was evaluated at the end of follow-up. The general information questionnaire was designed by the researchers, the serum Hcy and VEGF levels and other laboratory indicators on the day of admission were detected, the effect of serum Hcy and VEGF levels on the therapeutic effect of Alginic sodium diester tablets in the treatment of patients with TIA was mainly analyzed on the day of admission. RESULTS: Among 160 patients with TIA, 20 cases were ineffective, with the ineffective rate was 12. 50%; the total cholesterol (TC), triglyceride ( TG), Hcy, VEGF, high-shear whole blood viscosity ( HWBV), low-shear whole blood viscosity (LWBV) and plasma viscosity (PV) levels in the ineffective group on the day of admission were higher than those in the effective group, and the high density lipoprotein cholesterol ( HDL-C) level was lower in the ineffective group, with statistically significant differences (P<0. 05). There were no statistical significant differences in comparison of low density lipoprotein cholesterol ( LDL-C) and fibrinogen ( FIB) levels between two groups (P>0. 05). Results of multiple logistic regression and forest plot showed that the high levels of serum PV, LWBV, Hcy and VEGF on the day of admission were the risk factors of the ineffective treatment of Alginic sodium diester tablets for patients with TIA (OR>1, P<0. 05). The high level of serum HDL-C was the protective factor of the ineffective treatment for TIA patients (OR<1, P<0. 05). The receiver operating curve (ROC) was drawn to show that the AUC of serum Hcy and VEGF levels on the day of admission and the combined prediction of ineffective risk of Alginic sodium diester tablets for patients with TIA were 0. 753,0. 721 and 0. 822, respectively, which had a certain predictive value; the decision curve was drawn to show that within the threshold range from 0. 22 to 0. 58 and 0. 61 to 0. 98, the net yield of combined prediction model of serum Hcy and VEGF levels for the prediction of ineffective of Alginic sodium diester tablets for patients with TIA was better than that of serum Hcy or VEGF alone. CONCLUSIONS: The levels of serum Hcy and VEGF can affect the therapeutic effect of Alginic sodium diester tablets in patients with TIA. When the levels of serum Hcy and VEGF of patients increase, the risk of ineffective treatment increases. [ABSTRACT FROM AUTHOR]

Two sols of Al2 ()3 and TiO- were prepared by the sol-gel method, and coated on the surface of fly ash/loess support to prepare ceramic membranes. The thermal stability, crystal phase composition of the membrane materials were analyzed by thermogravimetric analyzer and X-ray diffractometer, and the microscopic morphology, porosity, membrane flux, and membrane flux recovery rate of the ceramic membranes were tested and characterized. The results show that the sintering temperature and drying conditions can effectively control the crystal phase change and integrity of the ceramic membrane. The AU()3 and Ti()2 membrane materials are dried at 5() °C and 25 °C, respectively, and sintered at 60() °C. The prepared AU ()3 membrane material is 7- AU ()3, and the TiO, membrane material is rutile TiO--The porosity of AU ()3 and TiO- ceramic membranes are 28.56% and 34.12%, the membrane fluxes are 1 ()92.67 L/(m2 • h) and 2 983.33 I>/(m2 • h), and the membrane flux recovery rates are 97% and 89%. Compared with the uncoated support, the permeability of AU()3 and Ti()2 ceramic membranes is reduced, but the anti-pollution ability is stronger due to its smooth and flat surface. [ABSTRACT FROM AUTHOR]

The study was to screen Bacillus antagonistic to Vibrio alginolyticus HY9901. A total of 117 strains of Bacillus were isolated from the root soil of mangrove in Zhanjiang, Guangdong province. A ZJ2009 strain with strong inhibitory effect on V. alginolyticus was obtained through primary and secondary screening. Its identification and biological characteristics were analyzed. The results showed that ZJ2009 strain was identified as Bacillus amyloliquefaciens. The optimal growth condition was BHI medium with 10% to 20% bottled load, initial pH value 6.0 to 8.0 and 1.5% NaCl added for more than 12 h. The strain had broad spectrum bacteriostasis, and failed to produce hemolytic loops, with less side effects on zebrafish and has a certain protection rate. The experiment indicates that Bacillus amyloliquefaciens can inhibit V. alginolyticus and provide a new idea for the prevention and control of vibriosis, which has potential development in the field of aquaculture. [ABSTRACT FROM AUTHOR]

Objective: To investigate the pathogenic bacteria composition, drug resistance characteristics and death influencing factors of patients with acute respiratory distress syndrome (ARDS) complicated with pulmonary infection. Methods: From January 2017 to January 2020, 98 patients with ARDS complicated with pulmonary infection who were treated in our hospital were included in this study. Sputum isolation and culture were carried out for all patients, and drug sensitivity test was carried out for pathogenic bacteria obtained from the separation. In addition, the number of patients who died within 28 days after admission was counted, univariate and multivariate Logistic regression analysis of influencing factors of death in patients with ARDS complicated with pulmonary infection. Results: A total of 163 strains of pathogenic bacteria were detected in 98 patients with ARDS complicated with pulmonary infection, from high to low, 123 strains of Gram-negative bacteria accounted for 75.46%, 33 strains of Gram-positive bacteria accounted for 20.25%, and 7 strains of fungus accounted for 4.29%. Among them, Gram-negative bacteria were mainly Pseudomonas aeruginosa (50 strains, accounting for 30.67%), A cinetobacter baumannii (32 strains, accounting for 19.63%) and Klebsiella pneumoniae (21 strains, accounting for 12.88% ). Pseudomonas aeruginosa, A cinetobacter baumannii, Staphylococcus aureus and hemolytic Staphylococcus had different degrees of resistance to common antibiotics, while Klebsiella pneumoniae had 0.00% resistance to Cefoperazone/sulbactam, cefepime, piperacillin/tazobactam and levofloxacin. Multivariate Logistic regression analysis showed that age ≥ 60 years, elevated C-reactive protein (CRP) and procalcitonin (PCT) levels, Acute physiological Function and Chronic health status scoring system Ⅱ (APACHE Ⅱ) score ≥ 25 scores, oxygenation index < 80 mmHg, length of Medical Intensive Care Unit (MICU) stay ≥ 10 d and mechanical ventilation time ≥ 6 d were all risk factors for death in patients with ARDS complicated with pulmonary infection (all OR>1, P<0.05). Conclusions: The pathogenic bacteria of patients with ARDS complicated with pulmonary infection are mainly Gram-negative bacteria, among which, Klebsiella pneumoniae has good sensitivity to Cefoperazone /sulbactam, cefepime, piperacillin/tazobactam and levofloxacin. Age, CRP, PCT level, APACHE Ⅱ score, oxygenation index, length of MICU stay and mechanical ventilation time are the influencing factors for the death of these patients. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To systematically review the efficacy and safety of Bufei Yishen method in the treatment of bronchial asthma in remission stage. METHODS: CNKI, Wanfang Data, VIP, PubMed and the Cochrane Library database were retrieved to collect the randomized controlled trials of Bufei Yishen method in the treatment of bronchial asthma in remission stage. The retrieval time was from the establishment of the database to Dec. 2021. Two evaluators screened the retrieved literature and extracted valid data respectively. Cochrane software was used to evaluate the literature quality, and RevMan 5. 3 software was used for Meta-analysis. RESULTS: Fifteen randomized controlled trials with a total of 1 305 patients were ultimately included. Meta-analysis results showed that Bufei Yishen method was better than conventional western medicine alone in improving the clinical efficiency, traditional Chinese medicine syndrome score, lung function, interleukin ( IL)-4 and IL-8 and other outcome indicators of bronchial asthma in remission stage, the differences were statistically significant(P< 0. 05). The incidence of adverse drug reactions of Bufei Yishen method was similar to that of conventional western medicine, and the incidence of adverse drug reactions of Bufei Yishen method was lower with higher safety. There was no definite evidence that Bufei Yishen method was superior to conventional western medicine in improving serum NO concentration and IL-5 level. CONCLUSIONS: The results suggest that the application of Bufei Yishen method in the treatment of bronchial asthma in remission stage is superior to conventional western medicine, and has certain feasibility and application value. However, due to the low quality of clinical evidence, it is necessary to perform high-quality randomized controlled trials for further verification. [ABSTRACT FROM AUTHOR]

BACKGROUND: In sports, side cutting is the most common attacking technique, which is influenced by landing mode and angle. The different patterns and angles of side cutting during landing may increase the risk of knee injury in the lower limbs. OBJECTIVE: To explain the impact of different foot strike patterns and angles in side cutting on the biomechanics of the lower extremity. METHODS: PubMed, Web of Science, and CNKI databases were searched for relevant studies published before August 2020. The keywords were “cutting maneuver; cutting maneuver angle; cutting foot strike; cutting biomechanics; change-of-direction” in Chinese and English, respectively. RESULTS AND CONCLUSION: The use of rear-foot strike patterns and larger angles for side cutting indicates an increased risk of anterior cruciate ligament injury. However, the current research only focuses on the analysis of foot strike patterns or larger angles alone, and rarely combines the foot strike patterns with the angle of side cutting. There is no clear conclusion about the interaction between foot strike patterns and angles in the side cutting, and there is not enough evidence to support that changing the foot strike pattern when the side cut angle increases will reduce the risk of lower extremity injury, which still requires a lot of research for in-depth demonstration. [ABSTRACT FROM AUTHOR]

Elastomers have a wide range of applications and a large market capacity, while general-purpose elastomers have the disadvantages of poor heat resistance and complex synthesis processes. Compared with traditional elastomer, polytriazole elastomer (PTAE) has the advantages of good heat resistance and simple synthesis, while the existed PTAE has poor mechanical properties (tensile strength ＜ 10 MPa). In order to obtain PTAE with excellent mechanical properties, a series of novel PTAEs with soft segments and hard segments alternately distributed along the main chain were synthesized via a metal-free 1,3-dipolar cycloaddition reaction. Based on the structural characteristics, alkynyl-terminated polyethylene glycol (DPPEG) with various amounts of polyether segments, biphenyl dibenzyl azide (BPDBA) with rigid aromatic rings and diethynylbenzene (m-DEB) were employed as soft segments, hard segments, and chain extender, respectively. The PTAEs possess good mechanical properties and thermal stability. With the increase of the DPPEG molar content, the tensile strength decreases from 16.27 MPa to 1.70 MPa, the elongation at break first increases and then decreases, ranging from 249% to 990%. And with the increase of DPPEG molecular weight, the tensile strength first increases and then decreases, ranging from 0.80 MPa to 22.5 MPa, and the elongation at break first decreases and then increases, ranging from 196% to 325%. Although the thermal stability of PTAE decreases with the increase of molar content and molecular weight of DPPEG, the decomposition temperature of 5% mass loss still exceeds 335 ℃, which proves that the structure has good thermal stability. The glass transition temperature of PTAE is less than 10 ℃ ，which can maintain elasticity at room temperature. [ABSTRACT FROM AUTHOR]

Objective: To investigate the levels of serum homocysteine(Hcy), D-Dimer(D-D) and B-type natriuretic peptide(BNP) in patients with nephrotic syndrome(NS) and their relationship with thromboembolism (TE). Methods: 241 patients with NS admitted to our hospital from January 2017 to January 2019 were selected and divided into TE group (n=56) and non TE group (n=185) according to whether they were complicated with TE or not. Another 65 healthy people were selected as the control group. The levels of serum Hcy, D-D and BNP in each group were compared.The influencing factors of NS patients complicated with TE were analyzed by multivariate logistic regression. Results: There were significant differences in serum Hcy, D-D and BNP levels among the three groups (P<0.05). The serum levels of Hcy, D-D and BNP in TE group were significantly higher than those in non TE group. The serum levels of Hcy, D-D and BNP in non TE group were significantly higher than those in control group (P<0.05). The proportion of history of hypertension,history of diuretic use, history of glucocorticoid use, BUN and 24hUPQ levels in TE group were significantly higher than those in non TE group (P<0.05). Multivariate logistic regression analysis showed that Hcy, D-D, BNP and 24hUPQ were independent influencing factors of NS patients complicated with TE (P<0.05). ROC curve showed that the AUC of serum Hcy+D-D+BNP level in diagnosing NS patients complicated with TE was significantly higher than that of serum Hcy, D-D and BNP alone, and the sensitivity and specificity were also higher than that of each index alone. Conclusion: The serum levels of Hcy, D-D and BNP in patients with NS were significantly increased, and further increased after combined with TE. The serum levels of Hcy, D-D and BNP were risk factors for the occurrence of TE in patients with NS, and combined detection can improve the diagnostic value. [ABSTRACT FROM AUTHOR]

In order to investigate the antifungal activities and the inhibitory mechanism of cantharidin derivatives against Sclerotinia sclerotiorum, the inhibitory effects of five halogenated aromatic amines(BY, BY-1, BY-2, BY-3 and BY-4) on the mycelial growth and the sclerotia formation of S. sclerotiorum were determined. The relative control efficacy against Sclerotinia rot on detached leaves of rapeseed and the antifungal mechanism of cantharidin derivatives were analyzed by measuring the mycelial morphology, the mycelial relative conductivity, the malondialdehyde content, the reduction of sugar content and soluble protein content, the activity of chitinase and the content of oxalic acid in the hypha treated with cantharidin derivatives. The results showed that five cantharidin derivatives significantly inhibited the mycelial growth of S. sclerotiorum, and the EC50 values of BY, BY-1, BY-2, BY-3 and BY-4 were 37.6, 8.78, 6.45, 12.2 and 50.4 μg/mL, respectively. The inhibitory effect of the derivative BY-2 on sclerotia formation was the strongest. And the inhibition rate was 86.52% at the concentration of 12.5 μg/mL. The treatment of the detached rapeseed leaves with the derivative BY-2 (100 μg/mL) afforded 97.71% relative control efficacy. These results indicated that the introduction of a chlorine atom at the C-3′ site of benzene ring could significantly improve the inhibitory activity of cantharidin against S. sclerotiorum. After the hypha was treated with cantharidin derivatives, the surface of mycelia turned rough, or dehydrated and shriveled, or deformed and expanded. The cell membrane permeability was increased, and the reducing sugar content and soluble protein content were decreased. Furthermore, the chitinase activity was also increased, and oxalic acid content was significantly decreased. Above all, the results showed that the cantharidin derivatives could effectively prevent and cure Sclerotinia stem rot, and provided theoretical basis for the development of biological fungicide. [ABSTRACT FROM AUTHOR]

Based on ArcGIS platform,Hancang River Basin in the east of Jinan City is taken as the research object,and the cumulative consumption distance model is used to analyze and optimize the landscape pattern of the basin to form the landscape ecological network.The catchment area unit and water system distribution of the research area are extracted by the watershed hydrological analysis method,and the ecological water network is constructed by combining the landscape network distribution of the basin.The ecological water network constructed according to the characteristics of landscape ecological pattern is closely related to the landscape elements in the basin,and promotes each other. The ecological flow in the study area runs smoothly,and the stability of water ecology is enhanced,thus forming a water ecological resilient space. [ABSTRACT FROM AUTHOR]

BACKGROUND: Previous studies found that exosomes from bone marrow mesenchymal stem cells could reduce the number of reactive astrocytes after spinal cord injury, thereby improving post-injury motor function. OBJECTIVE: To explore the role of bone marrow mesenchymal stem cell-derived exosomes in the activation of A1 astrocytes as well as in the repair of spinal cord injury. METHODS: Bone marrow mesenchymal stem cells from Sprague-Dawely rats were cultured, and then the exosomes from the cell supernatant were extracted and identified by ultracentrifugation. Eighty adult male Sprague-Dawley rats were randomly divided into sham operation group, spinal cord injury group (model group), bone marrow mesenchymal stem cell group (cell transplantation group) and exosome group (n=20/group). Except the sham operation group, an animal model of spinal cord injury was made in all the groups by Allen method. Two hours postoperatively, the equal volume of PBS, bone marrow mesenchymal stem cell suspension or bone marrow mesenchymal stem cell-derived exosome suspension was injected through the tail vein respectively, every 3 days, for three sessions. Basso, Beattie and Bresnahan scoring for motor function of the hind limb was performed preoperatively and and 1, 3, 7, 14, 28 days postoperatively. Hematoxylin-eosin staining and transmission electron microscope were used to observe the morphology of spinal cord tissue at 7 days postoperatively. Immunofluorescence staining was used to detect the number of A1 astrocytes, NeuN positive neurons and Caspase-3 positive apoptotic cells. ELISA assay was used to detect the levels of tumor necrosis factor-α and interleukin-1α in the spinal cord tissue. Western blot assay was used to determine relative protein expression levels of C3 and GFAP. RESULTS AND CONCLUSION: Exosomes from bone marrow mesenchymal stem cells showed a saucer-like appearance under transmission electron microscope, expressing exosome markers CD63 and CD9. The exosomes reduced inflammatory cell infiltration of spinal cord tissue, reduced myelin degeneration and axonal degeneration, reduced the expression levels of tumor necrosis factor-α, interleukin-1α and C3, reduced the number of A1 astrocytes, and reduced cell apoptosis, but increased the number of residual neurons, and improved limb motor function. To conclude, exosomes from bone marrow mesenchymal stem cells can reduce the activation of type A1 astrocytes in the spinal cord and promote the recovery of motor function after spinal cord injury. [ABSTRACT FROM AUTHOR]

Acute myocardial infarction (AMI) is a severe disease caused by the persistent coronary artery obstruction, posing a great threat to people's health. In recent years, Toll-like receptor 4 (TLR4), an important pattern recognition receptor, has been widely studied and found to play a crucial role in AMI. This review introduces the recent progress of TLR4 in regulating the progression and prognosis of AMI, and summarizes recent TLR4-targeted therapies using clinical drugs, TLR4 inhibitors, mesenchymal stem cells, and natural bioactive molecules, providing valuable insights for addressing myocardial damage caused by AMI and improving prognosis. [ABSTRACT FROM AUTHOR]

目的比较一次性和复用型活检枪在经皮肾穿刺活检术中的应用效果及价值,为临床医生合理选取手术器械提供帮助。方法选择2017年6月至 2019 年 6 月在该院拟行经皮肾穿刺活检术患者 53 例,将其随机分为A、B 组,分别采用 TRU-CORETM一次性肾穿刺活检枪和复用型Bard 自动活检枪进行肾穿刺活检手术,比较两组术后并发症、手术成功率及病理取材效果的差异。结果A组手术患者腰痛、肉眼血尿、肾周血肿的发生率与B组比较,差异均无统计学意义 (P>0.05);A、B 组病理取材良好分别为 24 例 (92.31%)、26例(96.30%),二者比较差异无统计学意义 (P>0.05)。结论在肾穿刺活检术中,复用型半自动活检枪的手术效果不低于一次性穿刺活检枪,但价格是后者的一半,值得在基层医院推广应用。 [ABSTRACT FROM AUTHOR]

目的探讨大剂量静脉补钠治疗老年低钠血症患者的疗效。方法选取2018年4月至2019年4月该院收治的老年低钠血症患者62例，随机分为A组（32例）和B组（30例）,分别采取外周静脉常规浓度和中心静脉高浓度补钠的治疗方案，对比2种不同方法的临床疗效。结果62例老年低钠血症患者实施补钠治疗72 h后，血清钠水平升至正常56例。2组患者治疗前后血清钠水平比较，差异均有统计学意义(P＜0.05)。B组患者低钠血症纠正率［100.00%(30/30)］高于A组［81.25%（26/32）］，差异有统计学意义(χ²=2.742, P=0.015)。结论经中心静脉高浓度 补钠是治疗老年尤其是重症低钠血症患者的首选方案，效果确切且显著，值得临床推广应用。 [ABSTRACT FROM AUTHOR]

Objective: To investigate whether there are pathogenic rare variants of gamma-aminobutyric acid type A (GABAA) receptor genes cluster in chromosome 15ql2 in children with autism. Methods: The GABAA receptor genes cluster in 96 Chinese Han autistic patients who fulfilled the criteria of the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition were sequenced by target sequencing and validated the rare variants u-sing Sanger sequencing. Furthermore, the sample size was expanded. The Continuity correction chi-square test was used to compare the difference of frequencies of these rare variations between 384 children with autism and 384 normal controls. Results: Through the target sequencing, 8 rare variants were found in the 96 autistic patients, including two rare missense mutations in GABRG3 (rs201602655 (p. Val233Met), rs201427468 (p. Pro365Ser)) and 6 rare mutations in noncoding regions of GABRB3. After validation of these results by Sanger sequencing and expansion of sample size, the frequency of heterozygous rs201606255 in autistic children was higher than that in controls (2. 1% vs. 0.3%, P <0.05). Functional prediction suggested that rs201602655 was a deleterious mutation that might affect the function of GABRG3. Conclusion: GABRG3 harbors rare mutations that changes amino acid in autistic children. GABRG3 may be a susceptible gene for autism and is involved in the pathogenesis of autism. [ABSTRACT FROM AUTHOR]

Intrusion detection systems are essential to protect the network security. However, it is hard for a traditional single algorithm to attain satisfied detection results for different attack classes. To solve this problem, this paper proposes an intrusion detection method based on Extreme Learning Machine (ELM) and modified K-means. ELM algorithm is optimized by Parametric Rectified Linear Unit (PReLU) activation function. The modified K-means algorithm can automatically select the initial centroids of clusters and the number of clusters by setting the distance threshold. Based on cascade algorithms, a hybrid intrusion detection method is designed based on improved ELM and modified K-means. The experimental results on NSL-KDD dataset show that, compared with other traditional algorithms such as BP neural network, Support Vector Machine (SVM) and ELM,the proposed method improves the detection results and reduces the false alarm rate. [ABSTRACT FROM AUTHOR]

Integrated water quality index(WPCNI)method was applied to evaluate the water quality in the mainstream of the Yellow River in seven monitoring sections from 2004 to 2016. The water pollution index(WPI)value was used as a sub-index, used principal component analysis to determine weight and introduced the Nemero Index. The WPCNI was used to analyze the overall state of pollution, temporal and spatial characteristics. The results show that the general water quality in the given period is good and 90. 93% of water body reaches Class Ⅲ standards; water quality has been improved during the study period; the proportion of water in class I-III is gradually increased; viewing from the spatial variation, the most polluted sections are Haibowan of Wuhai and Huajiangyingzi of Baotou, the main pollutant is NH3 -N and the water quality of upstream and downstream is better. [ABSTRACT FROM AUTHOR]

Objective: To investigate the current situation and intention of pharmacist appointment rotation in Nanyang area of Henan, so as to optimize the management of appointment rotation for pharmacists. Methods: Through sampling, a questionnaire survey on the intention of appointment rotation was conducted in 141 pharmacists from 13 hospitals in Nanyang area, and the data obtained from the survey were collected and analyzed by Excel software. Results: In the 13 hospital that underwent the survey, 12 hospitals (92.3%) had appointment rotation between the outpatient dispensary and inpatient dispensary, and 11 hospitals (84.6%) had appointment rotation between the post of the drug warehouses, but there was almost no appointment rotation in clinical pharmacy, pharmacy intravenous admixture service (PIVAS) and other units. The intention for appointment rotation was most strong in clinical pharmacy and outpatient pharmacy, where 129 (91.7%) and 113 (80.0%) medical personnel had the intention for rotation. PIVAS and inpatient dispensary were most preferred by surveyed pharmacists. There were 119 (84.7%) and 88(62.4%) pharmacists willing to go to the PIVAS and inpatient dispensary, respectively. The intention for appointment rotation among junior medical personnel was stronger than that among the older ones. Conclusion: With regard to the intention for appointment rotation, there exist certain differences or tendency among pharmacists, so the hospital pharmacy managers should make proper arrangement for them to have appointment rotation and should do as much as it can in accordance with the principle of overall consideration. [ABSTRACT FROM AUTHOR]

This article takes the agricultural transformation practice in M County as an example, starting from the perspective of farmers' differentiation and thinking, and considering the transformation of agricultural production and management models in the context of land transfer, exploring its transformation dynamics. Research has found that there are four modes of land transfer; spontaneous transfer, policy oriented accelerated transfer, capital oriented transfer, and market driven transfer. Agricultural production and operation entities have undergone four transformations; the agricultural production and operation mode has shown a diversified development trend of moderate scale operation, joint operation, trusteeship operation, and new agricultural operation in practice; policy assistance, market guidance, and technological support have played an important role in promoting the transformation of agricultural production and operation models. [ABSTRACT FROM AUTHOR]

BACKGROUND: Dexmedetomidine has the effect of anti-ischemia-reperfusion injury, but the comprehensive and systematic review of its signaling pathway is less. OBJECTIVE: To focus on the review of dexmedetomidine’s signaling pathway in the mechanisms of antioxidant stress, inhibition of inflammation, anti-apoptosis, autophagy, and so on. METHODS: The relevant articles on PubMed, CNKI, WanFang, and VIP databases were searched by computer with the key words “ischemia-reperfusion inquiry; dexmedetomidine; signal path; oxidative stress; inflammation; apoptosis” in Chinese and English. After excluding repetitive research and some basic articles with low correlation, 57 articles were finally included for review. RESULTS AND CONCLUSION: (1) Dexmedetomidine plays an important role in organ protection through many mechanisms, such as anti-oxidative stress injury, anti-inflammation, anti-apoptosis and autophagy. This involves many pathways, including Nrf2 and its downstream protein antioxidant stress pathway, Toll-like receptor 4 family and nuclear factor-κB-related anti-inflammatory pathway, JAK2/STAT3-related anti-inflammatory pathway, and cholinergic anti-inflammatory pathway, and the cholinergic pathway is the upstream mechanism of many nuclear factor-κB signaling pathways. (2) PI3K/Akt pathway plays different roles according to its activated downstream signals, inhibiting the activation of NLRP3 inflammatory body, activating signal molecules endothelial nitric oxide synthase, mammalian target of rapamycin, and hypoxia-inducible factor 1α to play an anti-inflammatory role, and activate Bad or Bax residues to play an antiapoptotic role, and PI3K/Akt activates glycogen synthetase kinase-3β. It can also play an anti-inflammatory and anti-apoptotic role. (3) Dexmedetomidine activates SIRT3 to mediate anti-apoptosis and inhibit endoplasmic reticulum stress to produce anti-apoptosis. (4) The detailed review of the anti-ischemiareperfusion injury signaling pathway of dexmedetomidine can provide a basis for future mechanism research and diagnosis and treatment decisions. [ABSTRACT FROM AUTHOR]

Objective To construct the miR-31 lentiviral vector and to investigate its effect on proliferation and migration of cervical cancer HeLa cells. Methods PCR was used to amplify the target gene which included gene miR-31. The target gene was designed with appropriate enzyme sites at the end of the primer pairs. We inserted the PCR products into linearization of lentiviral vector after the double digestion. Restriction endonuclease analysis and DNA sequencing had confirmed the sequence of the recombinant plasmid pCDH-CMV-MCS-EF1-copGFP-miR-31. HeLa cells were co-transfected with the lentiviral vectors pCDH-CMV-MCS-EF1-copGFP-miR-31，pPACKH1-GAG，pPACKH1-REV and pVSV-G，the titer of the lentivirus was detected. HeLa cells in the logarithmic phase were randomly divided into the control group without any treatment，the miR-31 group treated with lentivirus m iR-31，and the negative control group given empty lentivirus. The qPCR was used to detect the expression of miR-31 in HeLa cells of each group. The cell proliferation was detected by clone formation assay and MTT assay， and the cell migration ability was detected by Transwell assay. Results The recombinant lentivirus expression plasmid was constructed successfully，with lentivirus titer of 3 x 107 TU/mL. The expression of miR- 31 in HeLa cells of the miR-31 group was significantly higher than that of the other two groups ( all P < 0 . 05 ) ; no significant difference was found between the control group and the negative control group ( P > 0 . 05 ). Compared with the other two groups， the proliferation and migration abilities of HeLa cells were increased in the miR-31 group ( all P < 0 .05 ) ; no significant difference was found between the control group and the negative control group ( P > 0. 05 ) . Conclusion The miR-31 lentivirus expression plasmid is constructed successfully，and the proliferation and migration abilities of HeLa cells with stale expression of miR-31 are significantly enhanced. [ABSTRACT FROM AUTHOR]

ObjectiveTo investigate the clinical value of transient elastography (FibroScan) in the diagnosis of moderate-to-severe liver fibrosis in patients with chronic hepatitis B (CHB). MethodsA total of 245 patients with pathologically confirmed CHB by liver biopsy who underwent FibroScan and serological examinations in Shanghai Public Health Clinical Center from 2012 to 2015 were enrolled and divided into mild liver fibrosis group (S1-S2, 189 patients) and moderate-to-severe liver fibrosis group (S3-S3, 56 patients). The FibroScan value, aspartate aminotransferase-to-platelet ratio index (APRI) ［(aspartate aminotransferase/upper limit of normal×100)/platelet count］, and levels of hyaluronic acid (HA), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were measured. The t-test was used for comparison of normally distributed continuous data between groups, and the Mann-Whitney U rank sum test was used for comparison of non-normally distributed continuous data between groups. A logistic regression analysis was used for risk prediction. The receiver operating characteristic (ROC) curve, 95% confidence interval (95%CI), and area under the ROC curve (AUC) were used to investigate the accuracy of FibroScan in the diagnosis of moderate-to-severe liver fibrosis. The FibroScan value corresponding to the maximum of the sum of sensitivity and specificity was the optimal cut-off value for liver fibrosis. ResultsThere were significant differences in age, the levels of ALT, AST, and HA, APRI, and FibroScan value between the two groups (all P＜0.05). The univariate analysis showed that the severity of liver fibrosis was associated with the measured FibroScan value, HA level, and APRI (OR=2.56, 1.03, and 31.73, all P＜0.001), and the multivariate analysis showed that only FibroScan value was associated with moderate-to-severe liver fibrosis (OR=2.25, P＜0.001). The AUCs of FibroScan, HA, and APRI in the diagnosis of moderate-to-severe liver fibrosis were 0.985 (95%CI:0.969-1.000), 0.842 (95%CI:0.774-0.911), and 0.868 (95%CI:0.813-0.922), respectively, at cut-off values of 11.65, 104.4, and 0.69, respectively. In the diagnosis of moderate-to-severe liver fibrosis, FibroScan, HA, and APRI had sensitivities of 989%, 96.3%, and 96.3%, specificities of 85.7%, 55.4%, and 48.2%, positive predictive values of 95.9%, 87.9%, and 86.3%, and negative predictive values of 96.0%, 81.6%, and 79.4%. ConclusionFibroScan has a high clinical value in the diagnosis of moderate-to-severe liver fibrosis and plays an important role in early identification of liver fibrosis and accurate evaluation of fibrosis degree. [ABSTRACT FROM AUTHOR]

With the development of spectral technology, many researchers have done a lot of research on the nondestructive testing methods of nitrate content in leaves and have achieved some results. However, most of the studies have used visible and near infrared spectroscopy or mid infrared spectroscopy technology, and the chlorophyll fluorescence spectrum technology is still not used. Therefore, this paper used chlorophyll fluorescence spectrum analysis technology to study the relationship between nitrate content and fluorescence spectrum. With aerosol cultured lettuce as the research object, the chlorophyll fluorescence spectrum characteristic parameters were extracted to establish the function relationship between the characteristic parameters of chlorophyll fluorescence spectrum and the nitrate content of lettuce leaves, which laid a theoretical foundation for the rapid and nondestructive detection of nitrate content in lettuce leaves. The peak values of fluorescence intensity of 500-550, 650-715 and 715-800 nm were the characteristic parameters of chlorophyll fluorescence spectrum. It could be seen that the characteristic parameter of 500-550 nm was relatively weak, and it showed irregular variation with the change of nitrate content in lettuce leaves. Therefore, this band was not the desired band of this paper. The characteristic parameters of 650-715 and 715-800 nm showed clear and regular variation with the change of nitrate content in lettuce leaves, so they could be used for further study. In this paper, non dimensional treatment and standardized treatment of nitrate content in lettuce leaves and the characteristic parameters of 650-715 and 715-800 nm were in progress, and the linear fitting method was adopted. The results showed that the characteristic parameter of 650-715 nm and the nitrate content of lettuce leaves presented a significant linear relationship. The determination coefficient of regression model between them was 0.816 and the standard error was 0.147. Adding another variable in the regression model could improve the accuracy of the model a little; and the determination coefficient of regression model was 0.820 and the standard error was 0.146. And as the sample capacity was certain, the increase of the independent variable would cause the decrease of the degree of freedom, which then would affect the goodness of the fit of the regression model. Therefore, this paper chose the characteristic parameter of 650-715 nm to establish the regression model, which could well reflect the relationship between nitrate content of lettuce leaves and characteristic parameters of chlorophyll fluorescence spectrum. Thirty samples from the same batch were used as the calibration set of the regression model, and the regression model already established was used to predict the nitrate content of lettuce leaves. After verification, the determination coefficient between the predicted value and the measured value was 0.752, and the standard error was 0.172. The results show that the regression model has a good predictive effect on nitrate content of lettuce leaves and it can be used as the basis for rapid and nondestructive detection of nitrate content in lettuce leaves. [ABSTRACT FROM AUTHOR]

Objective To improve the hydrophobic properties of the ordered mesoporous carbon nanoparticles (MCN) by PVP or DSPE-mPEG２０００ modification, and to compare the effect on the dispersion and cytotoxicity of MCN before and after modification. Methods MCN was synthesized by low concentration hydrothermal method. The surface was modified by PVP or DSPE-mPEG２０００. The properties of the materials were characterized by transmission electron microscopy (TEM）, scanning electron microscopy (SEM）, nitrogen sorption analysis, particle size and infrared spectroscopy (IR）. The effect on the dispersion of MCN was investigated. CCK-８ method was used to investigate the cytotoxicity of the materials. Flow cytometry was used to investigate the effect of cell oxidative stress. Results The distribution of synthesized MCN was uniform, with average particle size about ９０ nm. The particle size and Zeta potential increased slightly after surface modification, while the dispersibility was significantly improved. No significant difference was observed in the toxicity of L９２９ and HeLa cells before and after modification. However, the modified MCN showed significant effect of reducing production of cellular reactive oxygen species. Conclusion Ordered mesoporous carbon nanoparticles with surface modified by PVP or DSPE-mPEG２０００ provide good bio- compatibility and can significantly reduce oxidative stress. [ABSTRACT FROM AUTHOR]