LncRNA SNHG12 调控 miR-138-5p/HIF-1α 轴改善缺氧/复氧人血管内皮 细胞损伤的研究.

Objective: To study the effect of Long non-coding RNA (LncRNA) small nucleolar RNA host gene 12 (SNHG12) regulating miR-138-5p/hypoxia inducible factor-1 (HIF-1α) axis on improving the damage of hypoxia/reoxygenation (H/R) human vascular endothelial cells. Methods: Human umbilical vein endothelial cells (HUVECs) were cultured in vitro and randomly divided into control group, H/R model group, H/R+LncRNA SNHG12 overexpression group, H/R+miR-138-5p mimics group, H/R+co-transfection group and H/R+co-transfection negative control group, each transfection group was transfected separately, and except for control group, the remaining groups were given hypoxia for 5 hours and then reoxygenated for 1 hour to induce the cell models, and then the cell viability of each group was detected by CCK-8 experiment; the cell apoptosis in each group was detected by flow cytometry experiment, and the apoptosis rate of each group was compared; the levels of reactive oxygen species (ROS), lactate dehydrogenase (LDH) and inflammatory factors IL-6, IL-17 and IL-18 in each group were measured by the kit; the expressions of miR-138-5p and HIF-1α mRNA in cells of each group were measured by real-time quantitative PCR (qRT-PCR) experiment; the expressions of apoptotic proteins caspase-9, Bcl-2-associated X protein (Bax) and HIF-1α in each group were evaluated by Western blot. Results: Compared with control group, the apoptosis rate, cellular ROS, LDH, IL-6, IL-17 and IL-18 levels, cellular HIF-1α mRNA and protein levels, cellular caspase-9, Bax and HIF-1α protein levels were increased in H/R model group (P<0.05), the cell viability and miR-138-5p level were decreased (P<0.05). Compared with H/R model group and H/R+co-transfection group, the cell viability, cell HIF-1α mRNA and protein levels were increased in H/R+LncRNA SNHG12 overexpression group (P<0.05), the apoptosis rate，cellular ROS, LDH, IL-6, IL-17 and IL-18 levels，cellular caspase-9 and Bax protein levels, and miR-138-5p level were decreased (P<0.05); the cell viability，cellular HIF-1α mRNA and protein levels were decreased in H/R+miR-138-5p mimics group (P<0.05), the apoptosis rate, cellular ROS, LDH, IL-6, IL-17 and IL-18 levels，cellular caspase-9 and Bax protein levels were increased (P<0.05). Compared with H/R model group, there was no significant difference in cell index levels between the H/R+co-transfection negative control group and the H/R+co-transfection group (P>0.05). Conclusion: LncRNA SNHG12 can upregulate HIF-1α expression by downregulating miR-138-5p expression, inhibit H/R-induced inflammation and oxidative stress in HUVECs, and reduce cell damage and apoptosis. [ABSTRACT FROM AUTHOR]