1. A Multi-Chamber Paper-Based Platform for the Detection of Amyloid β Oligomers 42 via Copper-Enhanced Gold Immunoblotting
- Author
-
Sungbo Cho and Le-Minh-Tu Phan
- Subjects
copper-enhanced gold nanoprobe ,Amyloid β ,Computer science ,Immunoblotting ,Nanoprobe ,Metal Nanoparticles ,Nanotechnology ,02 engineering and technology ,amyloid beta 42 oligomers ,Biosensing Techniques ,Microbiology ,01 natural sciences ,Biochemistry ,multi-chamber ,Amyloid Beta 42 ,wax printing ,Alzheimer Disease ,Limit of Detection ,Humans ,Medical diagnosis ,Molecular Biology ,Amyloid beta-Peptides ,Manufacturing process ,Communication ,010401 analytical chemistry ,Paper based ,021001 nanoscience & nanotechnology ,QR1-502 ,Peptide Fragments ,0104 chemical sciences ,Highly sensitive ,colorimetric immunoblot ,Gold ,0210 nano-technology ,Biosensor ,Alzheimer’s disease ,Biomarkers ,Copper - Abstract
The early diagnosis of Alzheimer’s disease (AD) remains a challenge for medical scientists worldwide, leading to a number of research efforts that focus on biosensor development for AD biomarkers. However, the application of these complicated biosensors is limited in medical diagnosis, due to the difficulties in robust sensing platform development, high costs, and the necessity for technical professionals. We successfully developed a robust straightforward manufacturing process for the fabrication of multi-chamber paper devices using the wax printing method and exploited it to detect amyloid beta 42 oligomers (AβO42, a significant biomarker of AD) using copper-enhanced gold nanoprobe colorimetric immunoblotting. Small hydrophilic reaction chambers could concentrate the target sample to the desired size to improve the sensing performance. The copper-enhanced gold nanoprobe immunoblot using the designed multi-chamber platform exhibited a highly sensitive performance with a limit of detection of 320 pg/mL by the naked eye and 23.7 pg/mL by a smartphone camera. This process from sensing manufacture to sensing conduction is simple to perform whenever medical technicians require time- and cost-savings, without complicated instruments or the need for technical professionals, making it feasible to serve as a diagnostic tool worldwide for the early monitoring of AD and scalable devices for the sensing application of various biomarkers in clinical settings.
- Published
- 2021