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Turntable Paper-Based Device to Detect Escherichia coli
- Source :
- Micromachines, Micromachines, Vol 12, Iss 194, p 194 (2021), Volume 12, Issue 2
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Escherichia coli has been known to cause a variety of infectious diseases. The conventional enzyme-linked immunosorbent assay (ELISA) is a well-known method widely used to diagnose a variety of infectious diseases. This method is expensive and requires considerable time and effort to conduct and complete multiple integral steps. We previously proposed the use of paper-based ELISA to rapidly detect the presence of E. coli. This approach has demonstrated utility for point-of-care (POC) urinary tract infection diagnoses. Paper-based ELISA, while advantageous, still requires the execution of several procedural steps. Here, we discuss the design and experimental implementation of a turntable paper-based device to simplify the paper-based ELISA protocols for the detection of E. coli. In this process, antibodies or reagents are preloaded onto zones of a paper-based device and allowed to dry before use. We successfully used this device to detect E. coli with a detection limit of 105 colony-forming units (colony-forming unit [CFU])/mL.
- Subjects :
- Computer science
lcsh:Mechanical engineering and machinery
medicine.disease_cause
01 natural sciences
Article
03 medical and health sciences
medicine
Escherichia coli
lcsh:TJ1-1570
Electrical and Electronic Engineering
030304 developmental biology
Point of care
0303 health sciences
turntable
business.industry
Mechanical Engineering
010401 analytical chemistry
Paper based
0104 chemical sciences
Control and Systems Engineering
point-of-care
business
Computer hardware
paper-based ELISA
Subjects
Details
- Language :
- English
- ISSN :
- 2072666X
- Volume :
- 12
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Micromachines
- Accession number :
- edsair.doi.dedup.....8560901ee925f7a9ed399c35f740a420