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The use of transferable cross species/ genera SSR markers is considered as cost effective strategy to ensure availability of markers in genomic resources of cotton. Forty one of 88 SSR primer pairs constitutes 46.59 % [24 from safflower i.e 27.27% and 17 from pulses i.e 19.32%] were found transferable as they amplified SSR priming genomic regions of both the genotypes. Out of these 88 markers, 27 (30.68%) were shown monomorphism and 14 (15.91%) were shown polymorphism. Among amplified markers, 45.83% and 17.65% polymorphism was observed by these heterolgous markers of safflower and pulses respectively.

Native fish species in Brazil are an asset in fish farming, but their natural stocks have been significantly reduced in recent years. To mitigate this negative impact, studies on fish conservation are being conducted and genetic tools for the discrimination of population parameters are increasingly achieving great importance. Current analysis evaluates a set of microsatellite heterologous primers in the jundiá (Rhamdia quelen) and in the piapara (Leporinus elongatus). Samples from the caudal fin of 15 broodstock from each species were analyzed. DNA extraction was performed with NaCl protocol and the integrity of the extracted DNA was checked with agarose gel 1%. Twenty primers developed for Piaractus mesopotamicus, Colossoma macropomum, Prochilodus lineatus, Brycon opalinus and Oreochromis niloticus were evaluated. Cross amplification of four primers of the B. opalinus and P. lineatus species (BoM12, Pli43 and Pli60 in R. quelen and BoM2, Pli43 and Pli60 in L. elongatus) was assessed. Primers of P. mesopotamicus, C. macropomum and O. niloticus showed no cross amplification in the two species analyzed. Results revealed the possibility of using the four amplified heterologous primers in genetic studies for R. quelen and L. elongatus. As espécies nativas de peixes no Brasil tem alto potencial para utilização dentro da piscicultura, porém, seus estoques naturais vêm sofrendo redução nos últimos anos. Para diminuir esse impacto negativo, estudos voltados à conservação estão sendo realizados e cada vez mais, a obtenção de ferramentas genéticas que permitam a discriminação de parâmetros populacionais toma maior importância. Objetivou-se através do presente estudo avaliar um conjunto de primers microssatélites heterólogos em jundiá (Rhamdia quelen) e piapara (Leporinus elongatus). Foram analisadas amostras de nadadeira caudal de 15 reprodutores de cada espécie. A extração de DNA foi realizada utilizando o protocolo com NaCl. A integridade do DNA extraído foi checada em gel de agarose 1%. Foram avaliados 20 primers desenvolvidos para as espécies: Piaractus mesopotamicus, Colossoma macropomum, Prochilodus lineatus, Brycon opalinus e Oreochromis niloticus. Foi observada amplificação cruzada de quatro primers das espécies B. opalinus e P. lineatus (BoM12, Pli43 e Pli60 em R. quelen e BoM2, Pli43 e Pli60 em L. elongatus). Os primers de P. mesopotamicus, C. macropomum e O. niloticus não mostraram amplificação cruzada nas duas espécies analisadas. Os resultados indicaram a possibilidade de uso dos três primers heterólogos amplificados em estudos genéticos em R. quelen e L. elongatus.

EnglishBrycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species. Key words: Brycon orbignyanus; conservation; molecular markers; heterologous primers portuguesA piracanjuba (Brycon orbignyanus) e um peixe de grande valor economico que nos ultimos anos tem apresentado uma reducao drastica em suas populacoes naturais. Nesse contexto, estudos de variabilidade genetica dos estoques naturais e nos programas de repovoamento sao fundamentais para adocao de medidas conservacionistas. O objetivo do presente trabalho foi verificar a amplificacao cruzada de primers heterologos em B. orbignyanus. Foram avaliados um total de 52 primers das especies Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum e Oreochromis niloticus. Os primers com melhor reprodutibilidade foram aplicados a uma amostra de 20 individuos e os parâmetros geneticos foram calculados. Nove primers apresentaram resultados satisfatorios de amplificacao cruzada com B. orbignyanus, sendo das especies Brycon opalinus (BoM5 e BoM13), Brycon hilarii (Bh5, Bh6, Bh8, Bh13 e Bh16), Brycon insignis (Bc48-10) e Prochilodus argenteus (Par80). Os primers de Piaractus mesopotamicus, Colossoma macropomum e Oreochromis niloticus nao apresentaram amplificacao ou apresentaram inespecificidade. Os resultados revelaram a possibilidade da utilizacao de primers de diferentes especies e genero em B. orbignyanus, o que facilita a realizacao de estudos geneticos nessa especie. Palavras-chave: Brycon orbignyanus; conservacao; marcadores moleculares; primers heterologos

Made available in DSpace on 2013-09-27T14:53:34Z (GMT). No. of bitstreams: 1 WOS000277326200022.pdf: 217877 bytes, checksum: 7458c9df5d00ed51f927bc0f7bfaa557 (MD5) Previous issue date: 2010-01-01 Made available in DSpace on 2013-09-30T18:03:32Z (GMT). No. of bitstreams: 1 WOS000277326200022.pdf: 217877 bytes, checksum: 7458c9df5d00ed51f927bc0f7bfaa557 (MD5) Previous issue date: 2010-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:19:10Z No. of bitstreams: 1 WOS000277326200022.pdf: 217877 bytes, checksum: 7458c9df5d00ed51f927bc0f7bfaa557 (MD5) Made available in DSpace on 2014-05-20T13:19:10Z (GMT). No. of bitstreams: 1 WOS000277326200022.pdf: 217877 bytes, checksum: 7458c9df5d00ed51f927bc0f7bfaa557 (MD5) Previous issue date: 2010-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Transferability of microsatellite loci between closely related species has been reported in several species. This helps reduce costs involved with the development of primers for newly investigated species. Fifteen microsatellite primers developed for Rangifer tarandus, Cervus elaphus, C. axis, and Moschus berezovskii were tested on five species of Brazilian brocket deer of the genus Mazama (M. americana, M. bororo, M. gouazoubira, M. nana, and M. nemorivaga). These primers were tested with DNA extracted from blood samples of two individuals of each species obtained from the Nucleo de Pesquisa e Conservacao de Cervideos (NUPECCE) of Universidade Estadual Paulista (UNESP). Fourteen of the 15 primers tested amplified microsatellite regions of all five species of Mazama, confirmed by sequencing of the amplified fragments. We conclude that these primers could be used for population studies of brocket deer. Univ Estadual Paulista, Dept Zootecnia, Nucleo Pesquisa & Conservacao Cervideos, Fac Ciencias Agr & Vet Jaboticabal, Jaboticabal, SP, Brazil Univ Estadual Paulista, Dept Zootecnia, Nucleo Pesquisa & Conservacao Cervideos, Fac Ciencias Agr & Vet Jaboticabal, Jaboticabal, SP, Brazil