Back to Search Start Over

Cross-amplification of heterologous microsatellite markers in Piracanjuba

Authors :
Pedro Luiz de Castro
Ricardo Pereira Ribeiro
Silvio Carlos Alves dos Santos
Elenice Souza dos Reis Goes
Felipe Pinheiro de Souza
Angela Rocio Poveda-Parra
Lauro Vargas
Angela Maria Urrea-Rojas
Nelson Mauricio Lopera-Barrero
Source :
Ciência Rural, Vol 47, Iss 12 (2017)
Publication Year :
2017
Publisher :
Universidade Federal de Santa Maria, 2017.

Abstract

ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species.

Details

Language :
English, Portuguese
ISSN :
16784596 and 01038478
Volume :
47
Issue :
12
Database :
Directory of Open Access Journals
Journal :
Ciência Rural
Publication Type :
Academic Journal
Accession number :
edsdoj.9685f896856c4706a98ce53a7a18c7e3
Document Type :
article
Full Text :
https://doi.org/10.1590/0103-8478cr20170374