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The article presents the results of experimental testing of the technique of tomographic reconstruction of circular birefringence maps of molecular complexes in a digital histological study of the age of damage to tissues of internal organs (brain, liver and kidney) of temporary monitoring of the mean value, dispersion, asymmetry and kurtosis, characterizing the distribution of the magnitude of the optical activity of histological sections of the brain, liver and kidney identification of the temporal extent of linear changes in statistical parameters and the accuracy of determining the duration of damage to human internal organs by digital histological methods of polarization reconstruction (tomography) of circular birefringence of molecular complexes. Aim of the work. Development of a technique for determining the duration of damage to human internal organs by digital histological methods of polarization reconstruction of circular birefringence of molecular complexes. Materials and methods. The object of the study was the histology of samples of human internal organs (brain, kidney and liver) with different duration of damage from 1 hour to 120 hours. For control, we used BT samples of those who died from coronary artery disease with different duration of damage from 1 hour to 120 hours. The studies were carried out using the technique of polarization reconstruction of circular birefringence of molecular complexes. Results. A set of treatment-relevant relationships between temporal changes in the statistical structure of topographic maps of circular birefringence of optically active molecular complexes of histological sections of human internal organs with different duration of damage and variations in the mean value, dispersion, asymmetry and kurtosis, characterizing the distribution of the value of this parameter of anisotropy, has been determined. Conclusions. A new original method has been developed for tomography of the optical activity of molecular complexes of tissues of human internal organs in a digital histological study of the age of damage to the tissues of the brain, liver and kidney, as well as the myocardium and lung tissue at a time interval of 1 hour. up to 120 hours

The article contains the results of a study of the effectiveness of forensic medical determination of the prescription of death by the method of differential Müller-matrix polarization-phase tomography, which is implemented by multichannel probing of rhizopolarization with laser beams of histological sections of the brain, myocardium, adrenal glands, liver and blood of the deceased and multichannel polarization filtration series images with algorithmic reproduction (reconstruction) of coordinate distributions (tomograms) of the average linear birefringence (LD) of polycrystalline networks. Aim of the work. Efficiency study by the method of differential Müller-matrix polarization-phase tomography for determining the duration of death. Materials and methods. The object of the study was the histology of samples of human internal organs (brain, myocardium, adrenal glands, and liver) with different duration of damage from 6 hours to 48 hours (we used BT samples from those who died from alcohol poisoning and carbon monoxide poisoning). For control, we used BT samples from those who died from ischemic heart disease with different duration of damage from 6 hours to 48 hours. The studies were carried out using the method of differential Muller-matrix polarization-phase tomography. Results. The range of linear change in the magnitude of the higher-order statistical moments, and accordingly the analytical determination of the DNS, lies in the range from 20 hours to 48 hours. The accuracy of determining the DNS is also significantly improved and amounts to 0.5 hours. Conclusion. The high efficiency of Muller matrix algorithmic reproduction of maps of average values of birefringence of the optically anisotropic component of biological preparations of the deceased was revealed.

Any physical object or medium is a complex, optically heterogeneous, structure. Such heterogeneity is characterized by the spatial distributions of the overall optical parameters (refractive indices and absorption) and their anisotropic components (linear and circular birefringence, linear and circular dichroism).

This section presents the results of the practical application of the theoretical correlation approach for combining the polarization.

Figures 3.1, 3.2, and Table 3.1 present the results of a wavelet analysis of polarization maps of microscopic images of a histological section of the skeletal muscle.

The analysis of the obtained data revealed a large degree of polarization uniformity of the microscopic image of the optically anisotropic spatially ordered fibrillar network of the skeletal muscle at small geometric scales, which are formed by optically active chains of myosin molecules in comparison with large window scales of the MHAT function. This fact is indicated by the different “structures” of the distribution of the values of the wavelet coefficients \(W_{a,b} \left\{ {V\left( {x,y} \right)} \right\}\) and their different-scale anharmonic distributions \(C_{a,b}\). As can be seen, at the small size window of the wavelet function, a rather harmonious (with a small modulation depth) modulation of the amplitude of the wavelet-coefficients \(W_{a = \hbox{min} ,b}\) of the coordinate distribution of the values of the CDMP modulus of the points of the microscopic image is formed. The opposite picture—the formation of anharmonic distributions (with a large modulation depth) of the amplitude of the wavelet-coefficients \(W_{a = \hbox{max} ,b}\)—takes place for the large-scale MHAT window—a function that distinguishes the manifestations of linear birefringence of the fibrillar network.

We introduce a Mueller-matrix imaging polarization-based approach for the quantitative digital screening of the polycrystalline structure of fibrillary-based biological tissues in vitro. The morphometric evaluation of histological sections of myocardium was performed utilizing the high-order statistical moments calculated based on the spatial distribution of linear and circular birefringence and dichroism obtained experimentally. We demonstrate that spatial distributions of phase of light and optical anisotropy of scattering inherent to fibrillar networks of myocardium at different necrotic stages can be effectively used as a quantitative marker of stages of myosin fibril degradation. Processing the images of phase of light scattered in biological tissues with high order statistical analysis provides a functional tool for the quantitative characterization of necrotic conditions of the myocardium.

The decomposition of the Mueller matrix of blood films has been carried out using differential matrices with polarized and depolarized parts. The use of a coherent reference wave is applied and the algorithm of digital holographic reconstruction of the field of complex amplitudes is used. On this basis, the 3D Mueller-matrix diffuse tomography method&mdash, the reconstruction of distributions of fluctuations of linear and circular birefringence of depolarizing polycrystalline films of human blood is analytically justified and experimentally tested. The dynamics of the change in the magnitude of the statistical moments of the first-fourth order, which characterize layer-by-layer distributions of fluctuations in the phase anisotropy of the blood film, is examined and analyzed. The most sensitive parameters for prostate cancer are the statistical moments of the third and fourth orders, which characterize the asymmetry and kurtosis of fluctuations in the linear and circular birefringence of blood films. The excellent accuracy of differentiation obtained polycrystalline films of blood from healthy donors and patients with cancer patients was achieved.