35 results on '"Yuenan Zhou"'
Search Results
2. Neofunctionalization of an ancient domain allows parasites to avoid intraspecific competition by manipulating host behaviour
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Jiani Chen, Gangqi Fang, Lan Pang, Yifeng Sheng, Qichao Zhang, Yuenan Zhou, Sicong Zhou, Yueqi Lu, Zhiguo Liu, Yixiang Zhang, Guiyun Li, Min Shi, Xuexin Chen, Shuai Zhan, and Jianhua Huang
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Science - Abstract
Evolutionary arms races can drive adaptations in hosts and parasites as well as among competing parasites. A combination of multi-omics and functional tests identifies a set of genes that allow a parasitic wasp to minimize intraspecific competition by inducing hosts to escape before more wasps can parasitize them.
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- 2021
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3. The Dual Functions of a Bracovirus C-Type Lectin in Caterpillar Immune Response Manipulation
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Xiaotong Wu, Zhiwei Wu, Xiqian Ye, Lan Pang, Yifeng Sheng, Zehua Wang, Yuenan Zhou, Jiachen Zhu, Rongmin Hu, Sicong Zhou, Jiani Chen, Zhizhi Wang, Min Shi, Jianhua Huang, and Xuexin Chen
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bracovirus ,C-type lectin ,immunosuppression ,hemocytes proliferation ,agglutination ,hypoimmunity ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Parasitoids are widespread in natural ecosystems and normally equipped with diverse viral factors to defeat host immune responses. On the other hand, parasitoids can enhance the antibacterial abilities and improve the hypoimmunity traits of parasitized hosts that may encounter pathogenic infections. These adaptive strategies guarantee the survival of parasitoid offspring, yet their underlying mechanisms are poorly understood. Here, we focused on Cotesia vestalis, an endoparasitoid of the diamondback moth Plutella xylostella, and found that C. vestalis parasitization decreases the number of host hemocytes, leading to disruption of the encapsulation reaction. We further found that one bracovirus C-type lectin gene, CvBV_28-1, is highly expressed in the hemocytes of parasitized hosts and participates in suppressing the proliferation rate of host hemocytes, which in turn reduces their population and represses the process of encapsulation. Moreover, CvBV_28-1 presents a classical bacterial clearance ability via the agglutination response in a Ca2+-dependent manner in response to gram-positive bacteria. Our study provides insights into the innovative strategy of a parasitoid-derived viral gene that has dual functions to manipulate host immunity for a successful parasitism.
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- 2022
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4. Two novel venom proteins underlie divergent parasitic strategies between a generalist and a specialist parasite
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Jianhua Huang, Jiani Chen, Gangqi Fang, Lan Pang, Sicong Zhou, Yuenan Zhou, Zhongqiu Pan, Qichao Zhang, Yifeng Sheng, Yueqi Lu, Zhiguo Liu, Yixiang Zhang, Guiyun Li, Min Shi, Xuexin Chen, and Shuai Zhan
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Science - Abstract
Parasitism is a widespread evolutionary strategy. A study that spans functional and evolutionary genomics identifies the molecular basis and history underlying two genes that have mediated divergent parasitic strategies (specialist vs generalist) between two sister species of parasitoid wasp.
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- 2021
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5. The genomes of two parasitic wasps that parasitize the diamondback moth
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Min Shi, Zhizhi Wang, Xiqian Ye, Hongqing Xie, Fei Li, Xiaoxiao Hu, Zehua Wang, Chuanlin Yin, Yuenan Zhou, Qijuan Gu, Jiani Zou, Leqing Zhan, Yuan Yao, Jian Yang, Shujun Wei, Rongmin Hu, Dianhao Guo, Jiangyan Zhu, Yanping Wang, Jianhua Huang, Francesco Pennacchio, Michael R. Strand, and Xuexin Chen
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Cotesia vestalis ,Diadromus collaris ,Parasitic wasps ,Genome ,Transcriptome ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Parasitic insects are well-known biological control agents for arthropod pests worldwide. They are capable of regulating their host’s physiology, development and behaviour. However, many of the molecular mechanisms involved in host-parasitoid interaction remain unknown. Results We sequenced the genomes of two parasitic wasps (Cotesia vestalis, and Diadromus collaris) that parasitize the diamondback moth Plutella xylostella using Illumina and Pacbio sequencing platforms. Genome assembly using SOAPdenovo produced a 178 Mb draft genome for C. vestalis and a 399 Mb draft genome for D. collaris. A total set that contained 11,278 and 15,328 protein-coding genes for C. vestalis and D. collaris, respectively, were predicted using evidence (homology-based and transcriptome-based) and de novo prediction methodology. Phylogenetic analysis showed that the braconid C. vestalis and the ichneumonid D. collaris diverged approximately 124 million years ago. These two wasps exhibit gene gains and losses that in some cases reflect their shared life history as parasitic wasps and in other cases are unique to particular species. Gene families with functions in development, nutrient acquisition from hosts, and metabolism have expanded in each wasp species, while genes required for biosynthesis of some amino acids and steroids have been lost, since these nutrients can be directly obtained from the host. Both wasp species encode a relative higher number of neprilysins (NEPs) thus far reported in arthropod genomes while several genes encoding immune-related proteins and detoxification enzymes were lost in both wasp genomes. Conclusions We present the annotated genome sequence of two parasitic wasps C. vestalis and D. collaris, which parasitize a common host, the diamondback moth, P. xylostella. These data will provide a fundamental source for studying the mechanism of host control and will be used in parasitoid comparative genomics to study the origin and diversification of the parasitic lifestyle.
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- 2019
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6. Bracoviruses recruit host integrases for their integration into caterpillar's genome.
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Zehua Wang, Xiqian Ye, Yuenan Zhou, Xiaotong Wu, Rongmin Hu, Jiachen Zhu, Ting Chen, Elisabeth Huguet, Min Shi, Jean-Michel Drezen, Jianhua Huang, and Xuexin Chen
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Genetics ,QH426-470 - Abstract
Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown. Here, we find that Cotesia vestalis bracovirus (CvBV), a polydnavirus of the parasitic wasp C. vestalis (Haliday), integrates its DNA circles into host Plutella xylostella (L.) genome by two distinct strategies, conservatively and randomly, through high-throughput sequencing analysis. We confirmed that the conservatively integrating circles contain an essential "8+5" nucleotides motif which is required for integration. Then we find CvBV circles are integrated into the caterpillar's genome in three temporal patterns, the early, mid and late stage-integration. We further identify that three CvBV-encoded integrases are responsible for some, but not all of the virus circle integrations, indeed they mainly participate in the processes of early stage-integration. Strikingly, we find two P. xylostella retroviral integrases (PxIN1 and PxIN2) are highly induced upon wasp parasitism, and PxIN1 is crucial for integration of some other early-integrated CvBV circles, such as CvBV_04, CvBV_12 and CvBV_24, while PxIN2 is important for integration of a late-integrated CvBV circle, CvBV_21. Our data uncover a novel mechanism in which CvBV integrates into the infected host genome, not only by utilizing its own integrases, but also by recruiting host enzymes. These findings will strongly deepen our understanding of how bracoviruses regulate and integrate into their hosts.
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- 2021
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7. Characterization of Molting Process during the Different Developmental Stages of the Diamondback Moth Plutella xylostella
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Licheng Gu, Zhiwei Wu, Xiaotong Wu, Yuenan Zhou, Pei Yang, Xiqian Ye, Min Shi, Jianhua Huang, and Xuexin Chen
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Plutella xylostella ,behavioral sequence ,molting ,ecdysis ,morphological characters ,Science - Abstract
The molting process of the lepidopteran insects is observed for many species. However, the detailed description of the morphological transformation and behavioral sequence during molting are rarely provided and visualized. Here, we described the molting process of the diamondback moth Plutella xylostella by providing the duration and photographic details of staging criteria of each stage using stereo microscopy and a digital video camera. We divided the morphological transformation of egg development and hatching into five stages, the larval–larval ecdysis and the larval–pupal metamorphosis into five stages, the pupal development and eclosion into three stages, and the post-eclosion behavior into four stages. Several new characters in the molting process that were not previously described in other lepidopteran insects were found, i.e., the larvae contracted anterior-posteriorly then dorsal-ventrally during pre-ecdysis, and the antennae waved backward then forward in the post-eclosion behavior. Our findings will deepen the knowledge of the molting biology of lepidopteran insects and facilitate the study of the underlying mechanisms.
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- 2022
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8. Comparative Transcriptome Analysis Reveals Sex-Based Differences during the Development of the Adult Parasitic Wasp Cotesia vestalis (Hymenoptera: Braconidae)
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Yuenan Zhou, Pei Yang, Shuang Xie, Min Shi, Jianhua Huang, Zhizhi Wang, and Xuexin Chen
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Cotesia vestalis ,Iso-Seq ,RNA-Seq ,sex-biased gene expression ,lncRNA ,alternative splicing ,Genetics ,QH426-470 - Abstract
The endoparasitic wasp Cotesia vestalis is an important biological agent for controlling the population of Plutella xylostella, a major pest of cruciferous crops worldwide. Though the genome of C. vestalis has recently been reported, molecular mechanisms associated with sexual development have not been comprehensively studied. Here, we combined PacBio Iso-Seq and Illumina RNA-Seq to perform genome-wide profiling of pharate adult and adult development of male and female C. vestalis. Taking advantage of Iso-Seq full-length reads, we identified 14,466 novel transcripts as well as 8770 lncRNAs, with many lncRNAs showing a sex- and stage-specific expression pattern. The differentially expressed gene (DEG) analyses showed 2125 stage-specific and 326 sex-specific expressed genes. We also found that 4819 genes showed 11,856 alternative splicing events through combining the Iso-Seq and RNA-Seq data. The results of comparative analyses showed that most genes were alternatively spliced across developmental stages, and alternative splicing (AS) events were more prevalent in females than in males. Furthermore, we identified six sex-determining genes in this parasitic wasp and verified their sex-specific alternative splicing profiles. Specifically, the characterization of feminizer and doublesex splicing between male and female implies a conserved regulation mechanism of sexual development in parasitic wasps.
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- 2021
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9. ACE: an efficient and sensitive tool to detect insecticide resistance-associated mutations in insect acetylcholinesterase from RNA-Seq data
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Dianhao Guo, Jiapeng Luo, Yuenan Zhou, Huamei Xiao, Kang He, Chuanlin Yin, Jianhua Xu, and Fei Li
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RNA-Seq data ,Insecticide resistance ,Mutations ,Ace ,Detection ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background Insecticide resistance is a substantial problem in controlling agricultural and medical pests. Detecting target site mutations is crucial to manage insecticide resistance. Though PCR-based methods have been widely used in this field, they are time-consuming and inefficient, and typically have a high false positive rate. Acetylcholinesterases (Ace) is the neural target of the widely used organophosphate (OP) and carbamate insecticides. However, there is not any software available to detect insecticide resistance associated mutations in RNA-Seq data at present. Results A computational pipeline ACE was developed to detect resistance mutations of ace in insect RNA-Seq data. Known ace resistance mutations were collected and used as a reference. We constructed a Web server for ACE, and the standalone software in both Linux and Windows versions is available for download. ACE was used to analyse 971 RNA-Seq data from 136 studies in 7 insect pests. The mutation frequency of each RNA-Seq dataset was calculated. The results indicated that the resistance frequency was 30%–44% in an eastern Ugandan Anopheles population, thus suggesting this resistance-conferring mutation has reached high frequency in these mosquitoes in Uganda. Analyses of RNA-Seq data from the diamondback moth Plutella xylostella indicated that the G227A mutation was positively related with resistance levels to organophosphate or carbamate insecticides. The wasp Nasonia vitripennis had a low frequency of resistant reads (
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- 2017
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10. Expression and functional characterization of odorant‐binding protein genes in the endoparasitic wasp Cotesia vestalis
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Wang Zehua, Sicong Zhou, Xue-Xin Chen, Yuenan Zhou, Fei Li, Pei Yang, Huang Jianhua, Min Shi, Shuang Xie, Lan Pang, and Jiani Chen
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Male ,media_common.quotation_subject ,Wasps ,Insect ,Vestalis ,Moths ,Receptors, Odorant ,General Biochemistry, Genetics and Molecular Biology ,RNA interference ,medicine ,Animals ,Cotesia vestalis ,Gene ,Phylogeny ,Ecology, Evolution, Behavior and Systematics ,media_common ,Genetics ,Olfactory receptor ,Diamondback moth ,biology ,fungi ,biology.organism_classification ,medicine.anatomical_structure ,Larva ,Insect Science ,Odorant-binding protein ,biology.protein ,Insect Proteins ,Female ,Agronomy and Crop Science - Abstract
Odorant-binding proteins (OBPs) are crucial in insect's olfactory perception, which participate in the initial step of odorant molecules transporting from the external environment to olfactory receptor neurons. To better understand the roles for OBPs in olfactory perception in Cotesia vestalis, a solitary larval endoparasitoid of diamondback moth, Plutella xylostella, we have comprehensively screened the genome of C. vestalis, and obtained 20 CvesOBPs, including 18 classic OBPs and two minus-C OBPs. Motif-pattern analysis indicates that the motifs of C. vestalis OBPs are highly conserved in Hymenoptera. The results of tissue expression analysis show that five OBPs (CvesOBP1/11/12/14/16) are highly expressed in male antennae, whereas six other OBP genes (CvesOBP7/8/13/17/18/19) are significantly transcriptionally enriched in female antennae. The results of RNA interference experiments for three most highly expressed OBP genes (CvesOBP17/18/19) in female antennae demonstrate that they are likely involved in parasitic processes of female wasps, as the wasps take a longer time to target the hosts when they are knocked down.
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- 2020
11. A teratocyte-specific serpin from the endoparasitoid wasp Cotesia vestalis inhibits the prophenoloxidase-activating system of its host Plutella xylostella
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Qijuan Gu, Zhiwei Wu, Yuenan Zhou, Zhizhi Wang, Min Shi, Jianhua Huang, and Xuexin Chen
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Enzyme Precursors ,Insect Science ,Larva ,Wasps ,Genetics ,Animals ,Moths ,Molecular Biology ,Catechol Oxidase ,Serpins ,Host-Parasite Interactions - Abstract
Many endoparasitoids adopt several parasitic factors, such as venom, polydnavirus and teratocytes, to suppress the immune response of their associated hosts including melanization for successful parasitism. A teratocyte-specific expressed serpin gene, designated as CvT-serpin6, was identified from the parasitoid Cotesia vestalis. The immunoblot result suggested that CvT-serpin6 was secreted into extracellular space. qPCR results showed that CvT-serpin6 was mainly transcribed at later stages of parasitism, and the transcriptional abundance of CvT-serpin6 in teratocytes was significantly increased in response to the challenge of bacteria. Inhibitory assay indicated that recombinant CvT-serpin6 (rCvT-serpin6) could inhibit the activation of Plutella xylostella prophenoloxidase and ultimately resulted in the inhibition of melanization in P. xylostella haemolymph. Furthermore, we confirmed that rCvT-serpin6 could form SDS-stable complexes with activated PxPAP1 and PxPAP3 in a dose-dependent manner. Altogether, our results further shed insight into the molecular mechanisms that teratocytes involved in controlling host immune response.
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- 2021
12. A serpin (CvT-serpin15) of teratocytes contributes to microbial-resistance in Plutella xylostella during Cotesia vestalis parasitism
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Qijuan, Gu, Zhiwei, Wu, Yuenan, Zhou, Zhizhi, Wang, Jianhua, Huang, Min, Shi, and Xuexin, Chen
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Lepidoptera ,Staphylococcus aureus ,Larva ,Wasps ,Animals ,Moths ,Serpins ,Host-Parasite Interactions - Abstract
Parasitic wasps are an important group of entomophagous insects for pest control. As parasitic wasps often lay eggs on or into their associated hosts, parasitoids evolve to utilize several factors including venom, polydnavirus (PDV) to alter host physiology for successful parasitism. Some taxa of endoparasitoids produce teratocytes, which are a type of cell that is released into host insects when wasp eggs hatch. Teratocytes display multifunction in parasitism such as host nutritional exploration, immune and developmental regulation, by secreting plenty of proteins into host hemocoel.A serpin (CvT-serpin15) secreted by teratocytes was characterized. QPCR results showed the expressional level of CvT-serpin15 was upregulated following bacterial challenges. Enzyme activity experiment indicated the recombinant CvT-serpin15 protein could interfere with the growth of Gram-positive bacteria Staphylococcus aureus. The survival rate assay demonstrated CvT-serpin15 increased survival rate of Plutella xylostella infected by S. aureus.CvT-serpin15 secreted by teratocytes would boost the host immune system when pathogens invade host hemocoel during parasitism, and ultimately protect the development of wasp larva from bacterial infection. © 2021 The Authors. Pest Management Science published by John WileySons Ltd on behalf of Society of Chemical Industry.
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- 2021
13. Protein Adsorption Behavior on the Surface of the Microfiltration Membrane Based on a Quartz Crystal Microbalance (QCM)
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Zhou Wang, Natsagdorj Khaliunaa, Yuenan Zhou, Tungalagtamir Bold, Zhan Wang, Yadong Kong, Khan Bushra, Qian Zhang, and Rooha Khurram
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Langmuir ,Membrane ,Materials science ,Adsorption ,Chemical engineering ,Fouling ,Ionic strength ,Freundlich equation ,Quartz crystal microbalance ,Protein adsorption - Abstract
How to fast and efficiently determinate the fouling behavior of the microfiltration membrane has great significance for the industrial membrane application. In this paper, the MF membrane was put on the surface of a gold-coated quartz crystal of QCM to study the adsorption behavior of protein at different conditions. The adsorbed mass increased with the increasing of concentration, ionic strength and temperature while decreased with the increasing of pH. Then the BSA adsorption results were compared with the corresponding membrane flux in dead-end cell at the identical conditions. Furthermore, the BSA adsorption process can be described by Langmuir and Freundlich isotherms very well. These results suggested that directly putting the membrane on the surface of a gold-coated quartz crystal of QCM can be used as a rapid and efficient approach to study protein fouling on the membrane surface. This approach using QCM and a small piece of the membrane could yield quantitative information for adsorption kinetics investigation and reduce the workload in large-scale industrial project.
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- 2018
14. Two novel venom proteins underlie divergent parasitic strategies between a generalist and a specialist parasite
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Xue-Xin Chen, Zhongqiu Pan, Qichao Zhang, Yifeng Sheng, Huang Jianhua, Yuenan Zhou, Sicong Zhou, Zhiguo Liu, Gangqi Fang, Min Shi, Lan Pang, Yueqi Lu, Guiyun Li, Shuai Zhan, Yixiang Zhang, and Jiani Chen
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Male ,0106 biological sciences ,0301 basic medicine ,Behavioural ecology ,Science ,media_common.quotation_subject ,Genome, Insect ,Wasps ,General Physics and Astronomy ,Parasitism ,Insect ,Generalist and specialist species ,010603 evolutionary biology ,01 natural sciences ,Article ,Evolutionary genetics ,Host Specificity ,General Biochemistry, Genetics and Molecular Biology ,Host-Parasite Interactions ,Parasitoid ,03 medical and health sciences ,Protein Domains ,Species Specificity ,Animals ,Parasites ,Phylogeny ,media_common ,Multidisciplinary ,biology ,Host (biology) ,fungi ,Immunity ,Mucins ,Animal Structures ,General Chemistry ,biology.organism_classification ,030104 developmental biology ,Evolutionary biology ,RNAi ,Horizontal gene transfer ,Insect Proteins ,Drosophila ,Host adaptation ,Adaptation ,Entomology - Abstract
Parasitoids are ubiquitous in natural ecosystems. Parasitic strategies are highly diverse among parasitoid species, yet their underlying genetic bases are poorly understood. Here, we focus on the divergent adaptation of a specialist and a generalist drosophilid parasitoids. We find that a novel protein (Lar) enables active immune suppression by lysing the host lymph glands, eventually leading to successful parasitism by the generalist. Meanwhile, another novel protein (Warm) contributes to a passive strategy by attaching the laid eggs to the gut and other organs of the host, leading to incomplete encapsulation and helping the specialist escape the host immune response. We find that these diverse parasitic strategies both originated from lateral gene transfer, followed with duplication and specialization, and that they might contribute to the shift in host ranges between parasitoids. Our results increase our understanding of how novel gene functions originate and how they contribute to host adaptation., Parasitism is a widespread evolutionary strategy. A study that spans functional and evolutionary genomics identifies the molecular basis and history underlying two genes that have mediated divergent parasitic strategies (specialist vs generalist) between two sister species of parasitoid wasp.
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- 2021
15. Bracoviruses recruit host integrases for their integration into caterpillar’s genome
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Wang Zehua, Xiaotong Wu, Jiachen Zhu, Yuenan Zhou, Ting Chen, Elisabeth Huguet, Rong-min Hu, Chen Xuexin, Ye Xiqian, Jean-Michel Drezen, Huang Jianhua, Min Shi, Zhejiang University, Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Université de Tours-Centre National de la Recherche Scientifique (CNRS)
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Cancer Research ,Life Cycles ,Hemocytes ,Wasps ,Moths ,QH426-470 ,Genome ,chemistry.chemical_compound ,White Blood Cells ,Medical Conditions ,Larvae ,Animal Cells ,Invertebrate Genomics ,Medicine and Health Sciences ,Cotesia vestalis ,Genetics (clinical) ,Genetic Interference ,Caterpillars ,Genetics ,0303 health sciences ,biology ,Polydnavirus ,030302 biochemistry & molecular biology ,Eukaryota ,Genomics ,3. Good health ,Insects ,Moths and Butterflies ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Cellular Types ,Bracovirus ,Research Article ,food.ingredient ,Arthropoda ,Immune Cells ,Immunology ,Human Genomics ,Host-Parasite Interactions ,03 medical and health sciences ,food ,Parasitic Diseases ,Animals ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,Blood Cells ,Integrases ,Host (biology) ,Organisms ,Biology and Life Sciences ,Cell Biology ,biology.organism_classification ,Invertebrates ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,chemistry ,Animal Genomics ,Polydnaviridae ,DNA, Viral ,Zoology ,Entomology ,DNA ,Developmental Biology - Abstract
Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown. Here, we find that Cotesia vestalis bracovirus (CvBV), a polydnavirus of the parasitic wasp C. vestalis (Haliday), integrates its DNA circles into host Plutella xylostella (L.) genome by two distinct strategies, conservatively and randomly, through high-throughput sequencing analysis. We confirmed that the conservatively integrating circles contain an essential “8+5” nucleotides motif which is required for integration. Then we find CvBV circles are integrated into the caterpillar’s genome in three temporal patterns, the early, mid and late stage-integration. We further identify that three CvBV-encoded integrases are responsible for some, but not all of the virus circle integrations, indeed they mainly participate in the processes of early stage-integration. Strikingly, we find two P. xylostella retroviral integrases (PxIN1 and PxIN2) are highly induced upon wasp parasitism, and PxIN1 is crucial for integration of some other early-integrated CvBV circles, such as CvBV_04, CvBV_12 and CvBV_24, while PxIN2 is important for integration of a late-integrated CvBV circle, CvBV_21. Our data uncover a novel mechanism in which CvBV integrates into the infected host genome, not only by utilizing its own integrases, but also by recruiting host enzymes. These findings will strongly deepen our understanding of how bracoviruses regulate and integrate into their hosts., Author summary Parasitoid wasps are a very large group of animals that live in or on the other arthropods. Polydnaviruses (PDVs) are a special kind of DNA virus associated parasitic wasps (primary hosts), which do not replicate in their infected caterpillar hosts (secondary hosts). During parasitoid oviposition, PDVs enter infected hosts and integrate their DNA into the genome of infected hosts, then their virulence genes begin to express and manipulate multiple biological process of hosts to fulfill all the requirements of parasitoids offspring. However, the mechanisms for integration remain largely unknown. Our research found the integration of PDVs is not only by utilizing its own integrases, but also by recruiting host enzymes. Here, we provide a new concept that host intergrases are involved in the integration of PDVs into host genome, and it greatly enhance our understanding of how PDVs regulate and integrate into their hosts, and may also inspire studies on how some DNA viruses may integrate their DNAs into human genome.
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- 2021
16. Neofunctionalization of an ancient domain allows parasites to avoid intraspecific competition by manipulating host behaviour
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Yuenan Zhou, Shuai Zhan, Zhiguo Liu, Gangqi Fang, Huang Jianhua, Xue-Xin Chen, Qichao Zhang, Yifeng Sheng, Yueqi Lu, Guiyun Li, Min Shi, Yixiang Zhang, Lan Pang, Jiani Chen, and Sicong Zhou
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Central Nervous System ,Male ,Behavioural ecology ,Science ,Population ,Wasps ,General Physics and Astronomy ,Gene Expression ,Escape response ,macromolecular substances ,Leptopilina ,Generalist and specialist species ,General Biochemistry, Genetics and Molecular Biology ,Intraspecific competition ,Article ,Evolutionary genetics ,Host-Parasite Interactions ,Biological Coevolution ,Eating ,Avoidance Learning ,Animals ,education ,education.field_of_study ,Multidisciplinary ,biology ,Behavior, Animal ,Host (biology) ,fungi ,GTPase-Activating Proteins ,food and beverages ,General Chemistry ,biology.organism_classification ,Drosophila melanogaster ,Evolutionary biology ,Larva ,Multigene Family ,RNAi ,Insect Proteins ,Neofunctionalization ,Female ,Adaptation ,Reactive Oxygen Species ,Entomology - Abstract
Intraspecific competition is a major force in mediating population dynamics, fuelling adaptation, and potentially leading to evolutionary diversification. Among the evolutionary arms races between parasites, one of the most fundamental and intriguing behavioural adaptations and counter-adaptations are superparasitism and superparasitism avoidance. However, the underlying mechanisms and ecological contexts of these phenomena remain underexplored. Here, we apply the Drosophila parasite Leptopilina boulardi as a study system and find that this solitary endoparasitic wasp provokes a host escape response for superparasitism avoidance. We combine multi-omics and in vivo functional studies to characterize a small set of RhoGAP domain-containing genes that mediate the parasite’s manipulation of host escape behaviour by inducing reactive oxygen species in the host central nervous system. We further uncover an evolutionary scenario in which neofunctionalization and specialization gave rise to the novel role of RhoGAP domain in avoiding superparasitism, with an ancestral origin prior to the divergence between Leptopilina specialist and generalist species. Our study suggests that superparasitism avoidance is adaptive for a parasite and adds to our understanding of how the molecular manipulation of host behaviour has evolved in this system., Evolutionary arms races can drive adaptations in hosts and parasites as well as among competing parasites. A combination of multi-omics and functional tests identifies a set of genes that allow a parasitic wasp to minimize intraspecific competition by inducing hosts to escape before more wasps can parasitize them.
- Published
- 2020
17. CLP gene family, a new gene family of Cotesia vestalis bracovirus inhibits melanization of Plutella xylostella hemolymph
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Min Shi, Yuenan Zhou, Xue-Xin Chen, Xiaotong Wu, Huang Jianhua, Ye Xiqian, Wang Zehua, and Pei Yang
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food.ingredient ,Genes, Viral ,Wasps ,Moths ,General Biochemistry, Genetics and Molecular Biology ,Parasitoid ,Microbiology ,Host-Parasite Interactions ,food ,Leucine ,Hemolymph ,Gene family ,Animals ,Cotesia vestalis ,Isoleucine ,Ecology, Evolution, Behavior and Systematics ,Melanins ,biology ,Monophenol Monooxygenase ,Pigmentation ,Polydnavirus ,fungi ,Plutella ,biology.organism_classification ,Drosophila melanogaster ,Cotesia ,Polydnaviridae ,Insect Science ,Larva ,Bracovirus ,Agronomy and Crop Science - Abstract
Polydnaviruses (PDVs) are obligatory symbionts of parasitoid wasps and play an important role in suppressing host immune defenses. Although PDV genes that inhibit host melanization are known in Microplitis bracovirus, the functional homologs in Cotesia bracoviruses remain unknown. Here, we find that Cotesia vestalis bracovirus (CvBV) can inhibit hemolymph melanization of its host, Plutella xylostella larvae, during the early stages of parasitization, and that overexpression of highly expressed CvBV genes reduced host phenoloxidase activity. Furthermore, CvBV-7-1 in particular reduced host phenoloxidase activity within 12 h, and the injection of anti-CvBV-7-1 antibody increased the melanization of parasitized host larvae. Further analyses showed that CvBV-7-1 and three homologs from other Cotesia bracoviruses possessed a C-terminal leucine/isoleucine-rich region and had a similar function in inhibiting melanization. Therefore, a new family of bracovirus genes was proposed and named as C-terminal Leucine/isoleucine-rich Protein (CLP). Ectopic expression of CvBV-7-1 in Drosophila hemocytes increased susceptibility to bacterial repression of melanization and reduced the melanotic encapsulation of parasitized D. melanogaster by the parasitoid Leptopilina boulardi. The formation rate of wasp pupae and the eclosion rate of C. vestalis were affected when the function of CvBV-7-1 was blocked. Our findings suggest that CLP genes from Cotesia bracoviruses encoded proteins that contain a C-terminal leucine/isoleucine-rich region and function as melanization inhibitors during the early stage of parasitization, which is important for successful parasitization.
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- 2020
18. Parasitic insect-derived miRNAs modulate host development
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Xue-Xin Chen, Yuenan Zhou, Rong-min Hu, Ye Xiqian, Zhizhi Wang, Wei Shujun, Chuanlin Yin, Fei Li, Dianhao Guo, Min Shi, Bo-Ying Zheng, Yanping Wang, Qi-juan Gu, Le-qing Zhan, Xiaotong Wu, Zou Jiani, Ting Chen, Xiao-dong Fang, Michael R. Strand, Wang Zehua, Huang Jianhua, and Na-na Hu
- Subjects
0301 basic medicine ,Receptors, Steroid ,Insecta ,food.ingredient ,Science ,media_common.quotation_subject ,Wasps ,General Physics and Astronomy ,Parasitism ,Insect ,Moths ,Article ,General Biochemistry, Genetics and Molecular Biology ,Host-Parasite Interactions ,03 medical and health sciences ,0302 clinical medicine ,food ,Animals ,Parasites ,Gene Silencing ,Cotesia vestalis ,lcsh:Science ,media_common ,Regulation of gene expression ,Multidisciplinary ,Diamondback moth ,biology ,Host (biology) ,fungi ,Gene Expression Regulation, Developmental ,General Chemistry ,biology.organism_classification ,Cell biology ,MicroRNAs ,030104 developmental biology ,Polydnaviridae ,Larva ,RNA Interference ,Female ,lcsh:Q ,Bracovirus ,Ecdysone receptor ,030217 neurology & neurosurgery - Abstract
Parasitic wasps produce several factors including venom, polydnaviruses (PDVs) and specialized wasp cells named teratocytes that benefit the survival of offspring by altering the physiology of hosts. However, the underlying molecular mechanisms for the alterations remain unclear. Here we find that the teratocytes of Cotesia vestalis, an endoparasitoid of the diamondback moth Plutella xylostella, and its associated bracovirus (CvBV) can produce miRNAs and deliver the products into the host via different ways. Certain miRNAs in the parasitized host are mainly produced by teratocytes, while the expression level of miRNAs encoded by CvBV can be 100-fold greater in parasitized hosts than non-parasitized ones. We further show that one teratocyte-produced miRNA (Cve-miR-281-3p) and one CvBV-produced miRNA (Cve-miR-novel22-5p-1) arrest host growth by modulating expression of the host ecdysone receptor (EcR). Altogether, our results show the first evidence of cross-species regulation by miRNAs in animal parasitism and their possible function in the alteration of host physiology during parasitism., The moth Plutella xylostella during its larval stage is the host of the endoparasitic wasp Cotesia vestalis. Here the authors show that the parasitoids deliver microRNAs to their hosts through their symbiotic virus and specialized cells leading to induced developmental delay.
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- 2018
19. ACE: an efficient and sensitive tool to detect insecticide resistance-associated mutations in insect acetylcholinesterase from RNA-Seq data
- Author
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Fei Li, Yuenan Zhou, Kang He, Jiapeng Luo, Huamei Xiao, Jianhua Xu, Chuanlin Yin, and Dianhao Guo
- Subjects
0106 biological sciences ,0301 basic medicine ,Insecticides ,medicine.medical_treatment ,Moths ,medicine.disease_cause ,Chilo suppressalis ,01 natural sciences ,Biochemistry ,Nasonia vitripennis ,Structural Biology ,Mutation frequency ,lcsh:QH301-705.5 ,Phylogeny ,Genetics ,Mutation ,education.field_of_study ,Diamondback moth ,biology ,Methodology Article ,Applied Mathematics ,Anopheles ,Organophosphates ,Computer Science Applications ,Detection ,Acetylcholinesterase ,lcsh:R858-859.7 ,Mutations ,Carbamate ,Insecticide resistance ,Population ,lcsh:Computer applications to medicine. Medical informatics ,RNA-Seq data ,03 medical and health sciences ,medicine ,Animals ,education ,Molecular Biology ,Ace ,Sequence Analysis, RNA ,business.industry ,biology.organism_classification ,Biotechnology ,010602 entomology ,030104 developmental biology ,lcsh:Biology (General) ,RNA ,business ,Sequence Alignment ,Software - Abstract
Background Insecticide resistance is a substantial problem in controlling agricultural and medical pests. Detecting target site mutations is crucial to manage insecticide resistance. Though PCR-based methods have been widely used in this field, they are time-consuming and inefficient, and typically have a high false positive rate. Acetylcholinesterases (Ace) is the neural target of the widely used organophosphate (OP) and carbamate insecticides. However, there is not any software available to detect insecticide resistance associated mutations in RNA-Seq data at present. Results A computational pipeline ACE was developed to detect resistance mutations of ace in insect RNA-Seq data. Known ace resistance mutations were collected and used as a reference. We constructed a Web server for ACE, and the standalone software in both Linux and Windows versions is available for download. ACE was used to analyse 971 RNA-Seq data from 136 studies in 7 insect pests. The mutation frequency of each RNA-Seq dataset was calculated. The results indicated that the resistance frequency was 30%–44% in an eastern Ugandan Anopheles population, thus suggesting this resistance-conferring mutation has reached high frequency in these mosquitoes in Uganda. Analyses of RNA-Seq data from the diamondback moth Plutella xylostella indicated that the G227A mutation was positively related with resistance levels to organophosphate or carbamate insecticides. The wasp Nasonia vitripennis had a low frequency of resistant reads (
- Published
- 2017
20. A trypsin inhibitor-like protein secreted by Cotesia vestalis teratocytes inhibits hemolymph prophenoloxidase activation of Plutella xylostella
- Author
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Xue-Xin Chen, Huang Jianhua, Shi-min Zhou, Min Shi, Qi-juan Gu, and Yuenan Zhou
- Subjects
0106 biological sciences ,0301 basic medicine ,Transcriptional Activation ,Physiology ,Trypsin inhibitor ,Wasps ,chemical and pharmacologic phenomena ,01 natural sciences ,Host-Parasite Interactions ,03 medical and health sciences ,Immune system ,Hemolymph ,medicine ,Animals ,Amino Acid Sequence ,Cotesia vestalis ,Enzyme Precursors ,biology ,Host (biology) ,hemic and immune systems ,Prophenoloxidase ,biology.organism_classification ,Trypsin ,Cell biology ,010602 entomology ,030104 developmental biology ,Insect Science ,Larva ,Humoral immunity ,Insect Proteins ,Trypsin Inhibitors ,Sequence Alignment ,Catechol Oxidase ,medicine.drug - Abstract
To establish successful infections, endoparasitoid wasps must develop strategies to evade immune responses of the host. Here, we identified and characterized a teratocytes-expressed gene encoding a trypsin inhibitor-like protein containing a cysteine-rich domain from Cotesia vestalis, CvT-TIL. CvT-TIL had a high expression level during the later developmental stage of teratocytes and was secreted into host hemolymph. Further experiments showed CvT-TIL strongly suppressed the prophenoloxidase activation of host hemolymph in a dose-dependent manner by interacting with PxPAP3 of PO cascade. Our results not only provide evidence for an inhibition between CvT-TIL gene and the host's melanization activity, but also expand our knowledge about the mechanisms by which parasitoids regulate humoral immunity of the host.
- Published
- 2019
21. The genomes of two parasitic wasps that parasitize the diamondback moth
- Author
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Jian Yang, Min Shi, Qi-juan Gu, Yuenan Zhou, Hongqing Xie, Zhizhi Wang, Chuanlin Yin, Zou Jiani, Dianhao Guo, Le-qing Zhan, Fei Li, Xiaoxiao Hu, Yuan Yao, Wang Zehua, Xue-Xin Chen, Huang Jianhua, Rong-min Hu, Jiangyan Zhu, Ye Xiqian, Michael R. Strand, Wei Shujun, Yanping Wang, Francesco Pennacchio, and Pennacchio, Francesco
- Subjects
0106 biological sciences ,Cotesia vestalis, Diadromus collaris, Parasitic wasps, Genome, Transcriptome ,lcsh:QH426-470 ,lcsh:Biotechnology ,Genome, Insect ,Wasps ,Biological pest control ,Genes, Insect ,Vestalis ,Moths ,010603 evolutionary biology ,01 natural sciences ,Genome ,Parasitoid ,Parasitic wasps ,03 medical and health sciences ,lcsh:TP248.13-248.65 ,Genetics ,Animals ,Cotesia vestalis ,Phylogeny ,030304 developmental biology ,Whole genome sequencing ,Comparative genomics ,0303 health sciences ,Diamondback moth ,biology ,fungi ,Immunity ,biology.organism_classification ,lcsh:Genetics ,Evolutionary biology ,Multigene Family ,Diadromus collaris ,Transcriptome ,Research Article ,Biotechnology - Abstract
Background Parasitic insects are well-known biological control agents for arthropod pests worldwide. They are capable of regulating their host’s physiology, development and behaviour. However, many of the molecular mechanisms involved in host-parasitoid interaction remain unknown. Results We sequenced the genomes of two parasitic wasps (Cotesia vestalis, and Diadromus collaris) that parasitize the diamondback moth Plutella xylostella using Illumina and Pacbio sequencing platforms. Genome assembly using SOAPdenovo produced a 178 Mb draft genome for C. vestalis and a 399 Mb draft genome for D. collaris. A total set that contained 11,278 and 15,328 protein-coding genes for C. vestalis and D. collaris, respectively, were predicted using evidence (homology-based and transcriptome-based) and de novo prediction methodology. Phylogenetic analysis showed that the braconid C. vestalis and the ichneumonid D. collaris diverged approximately 124 million years ago. These two wasps exhibit gene gains and losses that in some cases reflect their shared life history as parasitic wasps and in other cases are unique to particular species. Gene families with functions in development, nutrient acquisition from hosts, and metabolism have expanded in each wasp species, while genes required for biosynthesis of some amino acids and steroids have been lost, since these nutrients can be directly obtained from the host. Both wasp species encode a relative higher number of neprilysins (NEPs) thus far reported in arthropod genomes while several genes encoding immune-related proteins and detoxification enzymes were lost in both wasp genomes. Conclusions We present the annotated genome sequence of two parasitic wasps C. vestalis and D. collaris, which parasitize a common host, the diamondback moth, P. xylostella. These data will provide a fundamental source for studying the mechanism of host control and will be used in parasitoid comparative genomics to study the origin and diversification of the parasitic lifestyle.
- Published
- 2019
22. The developmental transcriptome of Trichopria drosophilae (Hymenoptera: Diapriidae) and insights into cuticular protein genes
- Author
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Zhongqiu Pan, Jiani Chen, Min Shi, Lan Pang, Yuenan Zhou, Chaoqun Li, Huang Jianhua, Xue-Xin Chen, Ying Wang, and Sicong Zhou
- Subjects
animal structures ,Physiology ,Animal Scales ,Genes, Insect ,Hymenoptera ,Real-Time Polymerase Chain Reaction ,Biochemistry ,Transcriptome ,03 medical and health sciences ,Genetics ,Animals ,KEGG ,Drosophila suzukii ,Molecular Biology ,Gene ,030304 developmental biology ,0303 health sciences ,biology ,fungi ,030305 genetics & heredity ,RNA ,biology.organism_classification ,Diapriidae ,PEST analysis - Abstract
The pupal endoparasitoid wasp Trichopria drosophilae (Hymenoptera: Diapriidae) plays an important role in biological control of many frugivorous fruit fly species including Drosophila suzukii, a well-known invasive pest. Here, we report the transcriptomes of T. drosophilae among different developmental stages. A total of 601,148,438 high-quality reads were obtained and de novo assembled into 187,704 unigenes with an average length of 1096 bp. Among them, 21,735 unigenes were annotated into 52 Gene Ontology terms and 36,898 were assigned to 25 Cluster of Orthologous Groups categories, whereas 30,585 unigenes were mapped to 270 Kyoto Encyclopedia of Genes and Genomes different pathways. Numbers of differentially expressed genes were found through comparisons between different developmental stages. We further identified 137 cuticular protein genes (CPs) from T. drosophilae transcriptome, including 59 from CPR family, 2 from Tweedle family, 1 from CPF family, 46 from CPAP family, and 29 from other CP families. We analyzed expression patterns of the CPs at different developmental stages of T. drosophilae, and found some stage-specific CPs. Quantitative real-time PCR results confirmed RNA sequencing findings based on the relative expression levels of eight randomly selected CPs. This study provides a valuable transcriptomic resource for a comprehensive understanding of the development and physiology of T. drosophilae, and will help to improve their parasitism efficiency for biological control purposes.
- Published
- 2018
23. The prediction and recovery of the flux of PVDF membrane during dead-end microfiltration
- Author
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Lina Cheng, Yuenan Zhou, Kui Gao, Zhan Wang, Zhongya Zhu, Liping Liu, and Ximing Zhang
- Subjects
General Chemical Engineering ,Microfiltration ,Flux ,02 engineering and technology ,law.invention ,Inorganic Chemistry ,chemistry.chemical_compound ,020401 chemical engineering ,law ,0204 chemical engineering ,Suspension (vehicle) ,Waste Management and Disposal ,Filtration ,Chromatography ,Fouling ,Renewable Energy, Sustainability and the Environment ,Organic Chemistry ,Membrane fouling ,021001 nanoscience & nanotechnology ,Pollution ,Mixed liquor suspended solids ,Fuel Technology ,chemistry ,Chemical engineering ,Sodium hydroxide ,0210 nano-technology ,Biotechnology - Abstract
BACKGROUND This research focused on the fouling behavior of 0.1 µm PVDF membrane caused by activated sludge suspension in dead-end filtration mode and the chemical cleaning of the fouled PVDF membrane by alkaline solutions. A combined intermediate pore blockage and cake filtration model was established to describe flux decline behavior. A series of experiments were performed to validate the proposed model and to explore the impacts of operating conditions on flux decline and recovery. RESULTS Flux decline was much faster with increasing transmembrane pressure (TMP) and concentration of mixed liquor suspended solids (MLSS). The proposed model predictions in this paper showed good agreement with the experimental data. The cleaning effect of sodium hypochlorite solution was superior to that of sodium hydroxide solution. Temperature was the dominant factor affecting flux recovery in the cleaning operation and the optimum chemical cleaning operating conditions were as follows: CNaOCl= 1%, r = 300 rpm, t = 30 min, T = 318 K. CONCLUSIONS The combined model was valid in predicting the flux decline process and more accurate than classical fouling models under the same operating conditions. Flux recovery was significantly improved under optimized cleaning conditions. © 2015 Society of Chemical Industry
- Published
- 2015
24. Additional file 1: Table S1. of ACE: an efficient and sensitive tool to detect insecticide resistance-associated mutations in insect acetylcholinesterase from RNA-Seq data
- Author
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Dianhao Guo, Jiapeng Luo, Yuenan Zhou, Huamei Xiao, He, Kang, Chuanlin Yin, Jianhua Xu, and Li, Fei
- Subjects
animal structures ,fungi ,genetic processes ,information science ,natural sciences - Abstract
The NCBI accession numbers of insect ace-1 and ace-2 genes
- Published
- 2017
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25. Additional file 3: Table S3. of ACE: an efficient and sensitive tool to detect insecticide resistance-associated mutations in insect acetylcholinesterase from RNA-Seq data
- Author
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Dianhao Guo, Jiapeng Luo, Yuenan Zhou, Huamei Xiao, He, Kang, Chuanlin Yin, Jianhua Xu, and Li, Fei
- Subjects
genetic processes ,natural sciences - Abstract
The SRA accession numbers of 971 RNA-Seq data used for detecting mutations
- Published
- 2017
- Full Text
- View/download PDF
26. Preparation of porous membrane by combined use of thermally and chemical reaction-introduced nonsolvent-induced phase separations
- Author
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Ximing Zhang, Zhan Wang, Qian Zhang, Yuenan Zhou, Wentao Yang, and Lina Cheng
- Subjects
Sodium bicarbonate ,Aqueous solution ,Fouling ,Sodium ,Analytical chemistry ,Polyacrylonitrile ,chemistry.chemical_element ,Ocean Engineering ,Pollution ,Chemical reaction ,chemistry.chemical_compound ,Membrane ,chemistry ,Chemical engineering ,Coagulation (water treatment) ,Water Science and Technology - Abstract
Microporous polyacrylonitrile membranes were prepared via the combination of thermally and chemical reaction-introduced nonsolvent-induced phase separations (TIPS and CRINIPS). In the membrane preparation, glacial acetic acid (GA) and aqueous solution of 2 wt.% sodium bicarbonate were used as additive and coagulation media, respectively, and chemical reaction between GA and sodium bicarbonate resulted in uniform microporous membranes. Pore connectivity and asymmetry were significantly improved when coagulation bath temperature was lowered to 15°C. The fouling mechanisms of the prepared membrane were analyzed by nonlinear regression method and cake filtration model was the most appropriate for the membrane prepared via combining TIPS and CRINIPS. The pore structure was made connective and asymmetric by using a 15°C aqueous solution of 2 wt.% sodium bicarbonate as coagulation media in membrane precipitation process, and the average pore size reduced from about 0.125 to 0.097 μm while decreasing the ...
- Published
- 2013
27. Study on the control of pore sizes of membranes using chemical methods Part IV. The role of organic acids
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Na Chen, Qian Zhang, Xiao Ye, Yuenan Zhou, Wentao Yang, Zhan Wang, Ximing Zhang, Wenyue Dong, Longyue Shi, and Lina Cheng
- Subjects
chemistry.chemical_classification ,Fumaric acid ,Chromatography ,Mechanical Engineering ,General Chemical Engineering ,Polyacrylonitrile ,General Chemistry ,Polymer ,Chemical reaction ,chemistry.chemical_compound ,Acetic acid ,Membrane ,Chemical engineering ,chemistry ,General Materials Science ,Phase inversion (chemistry) ,Citric acid ,Water Science and Technology - Abstract
In this paper, polyacrylonitrile (PAN) porous membranes were prepared via combination of phase inversion and chemical reaction method with different organic acids (glacial acetic acid, fumaric acid and citric acid) as additive. The experimental results showed that pure water flux of the membrane decreased with increase in polymer concentration and membrane prepared with moderate polymer concentration presented better performance. The influence of different organic acids on the properties of the casting solution was examined by viscosity, and the relationship between viscosity and membrane performance was analyzed. The prepared membranes were characterized in terms of pure water flux, retention, pore size distribution and scanning electron microscopy (SEM) observation. It was found that the membrane, prepared with glacial acetic acid, has the typical asymmetric membrane morphology with optimal combination of narrow pore size distribution and better permeability. The contribution of different organic acids in casting solution to membrane performance during chemical reaction was quantitatively analyzed, and the effects of different organic acids on membrane structures were discussed. Meantime, compaction analysis demonstrated that the membranes prepared with glacial acetic acid had improvement in resistance against compaction compared to that prepared with other acids.
- Published
- 2013
28. Prediction of impacts of fabrication conditions on the filtration performance of homemade VC-co-VAc-OH microfiltration membrane by Support Vector Machine (SVM)
- Author
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Longyue Shi, Wentao Yang, Dong Wenyue, Zhan Wang, Jing Zhang, Lina Cheng, Yuenan Zhou, Xuejie Xi, and Na Chen
- Subjects
Fabrication ,Chromatography ,Materials science ,Precipitation (chemistry) ,information science ,Evaporation ,Flux ,Ocean Engineering ,Pollution ,law.invention ,Support vector machine ,Membrane ,law ,Relative humidity ,Biological system ,Filtration ,Water Science and Technology - Abstract
In this study, the support vector machine (SVM) model which was based on restricted data sets (the size of the training set is small or small training sample) was applied to predict the permeate flux and rejection of Bovine serum albumin (BSA) of homemade VC-co-VAc-OH microfiltration membrane as the function of fabrication conditions. The membrane preparation conditions (the solid content, the additive content, environmental temperature, the relative humidity, evaporation time of a volatile solvent, precipitation temperature, and precipitation time) were input variables; pure water flux and rejection of BSA were output variables. The results showed that the detailed relationships between fabrication conditions and filtration performance of the membranes could be established. Excellent agreements between the prediction of SVM model and the experiments validate that SVM model has sufficient accuracy. Furthermore, the results predicted by SVM model were compared with those predicted by artificial ne...
- Published
- 2013
29. Equilibrium and thermodynamic studies on adsorption of BSA using PVDF microfiltration membrane
- Author
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Wentao Yang, Jing Zhang, Xuejie Xi, Yuenan Zhou, Qian Zhang, and Zhan Wang
- Subjects
Langmuir ,Chromatography ,Aqueous solution ,Chemistry ,Mechanical Engineering ,General Chemical Engineering ,technology, industry, and agriculture ,General Chemistry ,Membrane bioreactor ,Polyvinylidene fluoride ,chemistry.chemical_compound ,Membrane ,Adsorption ,Chemical engineering ,Ionic strength ,General Materials Science ,Freundlich equation ,Water Science and Technology - Abstract
It was the aim of this work to investigate equilibrium and thermodynamic aspects for static adsorption of bovine serum albumin (BSA) as model protein of extracellular polymer substances (EPS) from membrane bioreactor (MBR) on hydrophilic polyvinylidene fluoride (PVDF) microfiltration membrane. In present paper, the influences of temperature, pH and ionic strength were studied and eight different isotherm models were used to fit equilibrium data by nonlinear regression method. The obtained results showed that the best-fitted adsorption isotherm models were in the order of: Langmuir and Freundlich > BET > Langmuir ≈ Redlich–Peterson ≈ Temkin > Freundlich > Fowler and Guggenheim (Hill–De Boer), of which, Langmuir–Freundlich isotherm is the best suitable. Thermodynamic investigation indicated that this process was not spontaneous and endothermic and maybe attributed to physicosorption, and the distribution of BSA adsorbed on PVDF membrane was more chaotic than that in the aqueous solution. The results from the static adsorption of BSA on PVDF membrane can be a useful tool to evaluate the behavior of membrane biofouling in MBR and also provide basis for performance comparison among different membranes.
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- 2012
30. The influence of operating conditions on the filtration behavior of actual extracellular polymeric substances (EPS) using dead-end membrane filtration cell
- Author
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Wenyue Dong, Qian Zhang, Yin Song, Liying Yang, Jing Zhang, Longyue Shi, Yuenan Zhou, Zhan Wang, and Mei Liu
- Subjects
Chromatography ,Chemistry ,Microfiltration ,Ocean Engineering ,Pollution ,Cross-flow filtration ,law.invention ,Activated sludge ,Extracellular polymeric substance ,Membrane ,Volume (thermodynamics) ,Dead end ,law ,Filtration ,Water Science and Technology - Abstract
The EPS solution extracted from the activated sludge of sequencing batch reactors (SBRs) by the formaldehyde–NaOH extraction method was filtered in dead-end cell with 0.1 μm PVDF micro filtration membrane under various operating conditions, and the filtration behaviors of actual EPS solution were investigated. The experimental results show that: firstly, the membrane filtration mechanism is governed by cake filtration, and the cake is compressible; secondly, the cake specific resistance increased with the increase of transmembrane pressure (TMP) and decreased as the concentration increased; thirdly, all operating conditions had a significant influence on the cumulative filtrate volume (CFV) of actual EPS solution; finally, the CFV increased with the rise of temperature and TMP, but decreased as EPS concentration increased. The sequence of influence degree of operating conditions is the temperature (38.1%) > the TMP (34.8%) > the EPS concentration (27.1%). A quantitative regression relationship be...
- Published
- 2012
31. Study of the contribution of the main pollutants in the oilfield polymer-flooding wastewater to the critical flux
- Author
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Liying Yang, Zhan Wang, Yuenan Zhou, Xiuyan Wang, Xuyan Wang, Xuejie Xi, and Wenjuan Li
- Subjects
Suspended solids ,Materials science ,Fouling ,Mechanical Engineering ,General Chemical Engineering ,Polyacrylamide ,Membrane fouling ,Environmental engineering ,Flux ,General Chemistry ,Pulp and paper industry ,Membrane technology ,law.invention ,chemistry.chemical_compound ,chemistry ,Wastewater ,law ,General Materials Science ,Filtration ,Water Science and Technology - Abstract
One of the major technical requirements of the crude oil exploitation industry is to minimize the concentration of hydrolyzed polyacrylamide (HPAM), crude oil and suspended solid (SS) in the oilfield polymer-flooding wastewater and realize the reuse of this wastewater. In this study, the membrane technology was used to achieve this goal in laboratory-scale dead-end test unit with flat polyvinylidene fluoride (PVDF) membrane (MW 100 kDa). We systematically inspected the membrane fouling mechanism, the variety of total membrane resistance, total fouling resistance, the dominant resistance, membrane fouling driving force and the filtration proceeds in the filtration process. The orthogonal method and multivariate linear regression method were applied to analyze the influencing degree of the main pollutant concentration on the critical flux. According to comparison of the average rates of change of the critical flux for the HPAM concentration, oil concentration and SS concentration in single solute solution, double solute solution and oilfield polymer-flooding wastewater, HPAM can decrease the average rate of change of the critical flux for other two solutes and has the crucial effect on the critical flux. The sequence of influence degree on the critical flux is the HPAM concentration (84.58%) > oil concentration (14.36%) > SS concentration (1.06%).
- Published
- 2011
32. Preparation of porous membrane by combined use of thermally and chemical reaction-introduced nonsolvent-induced phase separations.
- Author
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Wentao Yang, Zhan Wang, Yuenan Zhou, Lina Cheng, Qian Zhang, and Ximing Zhang
- Subjects
PHASE separation ,COAGULATION ,CHEMICAL reactions ,POROSITY ,SODIUM bicarbonate ,NONLINEAR regression ,AQUEOUS solutions - Abstract
Microporous polyacrylonitrile membranes were prepared via the combination of thermally and chemical reaction-introduced nonsolvent-induced phase separations (TIPS and CRINIPS). In the membrane preparation, glacial acetic acid (GA) and aqueous solution of 2 wt.% sodium bicarbonate were used as additive and coagulation media, respectively, and chemical reaction between GA and sodium bicarbonate resulted in uniform microporous membranes. Pore connectivity and asymmetry were significantly improved when coagulation bath temperature was lowered to 15˚C. The fouling mechanisms of the prepared membrane were analyzed by nonlinear regression method and cake filtration model was the most appropriate for the membrane prepared via combining TIPS and CRINIPS. The pore structure was made connective and asymmetric by using a 15˚C aqueous solution of 2 wt.% sodium bicarbonate as coagulation media in membrane precipitation process, and the average pore size reduced from about 0.125 to 0.097 μm while decreasing the glass plate temperature from 80 to 20˚C. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
33. The influence of operating conditions on the filtration behavior of actual extracellular polymeric substances (EPS) using dead-end membrane filtration cell.
- Author
-
Mei Liu, Zhan Wang, Longyue Shi, Yin Song, Wenyue Dong, Yuenan Zhou, Jing Zhang, Liying Yang, and Qian Zhang
- Subjects
MEMBRANE separation ,SEQUENCING batch reactor process ,REGRESSION analysis ,MICROFILTRATION ,FORMALDEHYDE - Abstract
The EPS solution extracted from the activated sludge of sequencing batch reactors (SBRs) by the formaldehyde-NaOH extraction method was filtered in dead-end cell with 0.1 μm PVDF micro-filtration membrane under various operating conditions, and the fi ltration behaviors of actual EPS solution were investigated. The experimental results show that: firstly, the membrane filtration mechanism is governed by cake filtration, and the cake is compressible; secondly, the cake specific resistance increased with the increase of transmembrane pressure (TMP) and decreased as the concentration increased; thirdly, all operating conditions had a significant influence on the cumulative filtrate volume (CFV) of actual EPS solution; finally, the CFV increased with the rise of temperature and TMP, but decreased as EPS concentration increased. The sequence of influence degree of operating conditions is the temperature (38.1%) > the TMP (34.8%) > the EPS concentration (27.1%). A quantitative regression relationship between the CFV and the temperature (T), TMP (ΔP) and EPS concentration (C) was obtained as follows: [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
34. Mathematical Modeling of Thin-layer Infrared Drying of Dewatered Municipal Sewage Sludge (DWMSS)
- Author
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Yiying Jin and Yuenan Zhou
- Subjects
Materials science ,Moisture ,Infrared ,020209 energy ,Environmental engineering ,Hasibuan and Daud model ,Municipal sewage ,02 engineering and technology ,Activation energy ,Thermal diffusivity ,Pulp and paper industry ,thin-layer drying ,activation energy ,Scientific method ,Sludge cake ,0202 electrical engineering, electronic engineering, information engineering ,General Earth and Planetary Sciences ,Nonlinear regression ,sludge cake ,moisture diffusivity ,General Environmental Science - Abstract
In this paper, thin-layer infrared drying behavior of dewatered municipal sewage sludge was systematically investigated. Drying experiments were conducted at drying temperatures from 65 to 135C and the thickness of sludge cake from 2 to 10 mm. Twenty thin-layer drying kinetic models were selected to model the drying process, and model parameters were evaluated by nonlinear regression method, of which, two different nonlinear error functions (R 2 , RSS) were examined to gauge the goodness-of-fit. The experimental results manifested that Hasibuan and Daud model is the best suitable model with highest R 2 values from 0.99951 to 0.99996 and lowest RSS values from 3.3×10 -4 to 0.00117 under our experimental conditions; drying rate increased with increase in drying temperature and decrease in the thickness of sludge cake; effective moisture diffusivity notably increased as drying temperature increased; activation energy for drying process was 34.9211 kJ/mol.
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35. Prediction of impacts of fabrication conditions on the filtration performance of homemade VC-co-VAc-OH microfiltration membrane by Support Vector Machine (SVM).
- Author
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Xuejie Xi, Zhan Wang, Jing Zhang, Yuenan Zhou, Na Chen, Longyue Shi, Dong Wenyue, Lina Cheng, and Wentao Yang
- Subjects
SUPPORT vector machines ,ARTIFICIAL neural networks ,SERUM albumin - Abstract
In this study, the support vector machine (SVM) model which was based on restricted data sets (the size of the training set is small or small training sample) was applied to predict the permeate flux and rejection of Bovine serum albumin (BSA) of homemade VC-co-VAc-OH microfiltration membrane as the function of fabrication conditions. The membrane preparation conditions (the solid content, the additive content, environmental temperature, the relative humidity, evaporation time of a volatile solvent, precipitation temperature, and precipitation time) were input variables; pure water flux and rejection of BSA were output variables. The results showed that the detailed relationships between fabrication conditions and filtration performance of the membranes could be established. Excellent agreements between the prediction of SVM model and the experiments validate that SVM model has sufficient accuracy. Furthermore, the results predicted by SVM model were compared with those predicted by artificial neural network (ANN) model which was widely used in the optimization of nonlinear relationships. It is found that the deviations of both the training and the predicting data obtained by SVM model are much smaller than those by ANN models. Hence, SVM model can be used as an efficient approach to optimize fabrication conditions of homemade VC-co-VAc-OH microfiltration membrane. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
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