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Expression and functional characterization of odorantā€binding protein genes in the endoparasitic wasp Cotesia vestalis

Authors :
Wang Zehua
Sicong Zhou
Xue-Xin Chen
Yuenan Zhou
Fei Li
Pei Yang
Huang Jianhua
Min Shi
Shuang Xie
Lan Pang
Jiani Chen
Source :
Insect Science. 28:1354-1368
Publication Year :
2020
Publisher :
Wiley, 2020.

Abstract

Odorant-binding proteins (OBPs) are crucial in insect's olfactory perception, which participate in the initial step of odorant molecules transporting from the external environment to olfactory receptor neurons. To better understand the roles for OBPs in olfactory perception in Cotesia vestalis, a solitary larval endoparasitoid of diamondback moth, Plutella xylostella, we have comprehensively screened the genome of C. vestalis, and obtained 20 CvesOBPs, including 18 classic OBPs and two minus-C OBPs. Motif-pattern analysis indicates that the motifs of C. vestalis OBPs are highly conserved in Hymenoptera. The results of tissue expression analysis show that five OBPs (CvesOBP1/11/12/14/16) are highly expressed in male antennae, whereas six other OBP genes (CvesOBP7/8/13/17/18/19) are significantly transcriptionally enriched in female antennae. The results of RNA interference experiments for three most highly expressed OBP genes (CvesOBP17/18/19) in female antennae demonstrate that they are likely involved in parasitic processes of female wasps, as the wasps take a longer time to target the hosts when they are knocked down.

Details

ISSN :
17447917 and 16729609
Volume :
28
Database :
OpenAIRE
Journal :
Insect Science
Accession number :
edsair.doi.dedup.....31812ac9446ead69fc077d84c6e6d96b