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Expression and functional characterization of odorantābinding protein genes in the endoparasitic wasp Cotesia vestalis
- Source :
- Insect Science. 28:1354-1368
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Odorant-binding proteins (OBPs) are crucial in insect's olfactory perception, which participate in the initial step of odorant molecules transporting from the external environment to olfactory receptor neurons. To better understand the roles for OBPs in olfactory perception in Cotesia vestalis, a solitary larval endoparasitoid of diamondback moth, Plutella xylostella, we have comprehensively screened the genome of C. vestalis, and obtained 20 CvesOBPs, including 18 classic OBPs and two minus-C OBPs. Motif-pattern analysis indicates that the motifs of C. vestalis OBPs are highly conserved in Hymenoptera. The results of tissue expression analysis show that five OBPs (CvesOBP1/11/12/14/16) are highly expressed in male antennae, whereas six other OBP genes (CvesOBP7/8/13/17/18/19) are significantly transcriptionally enriched in female antennae. The results of RNA interference experiments for three most highly expressed OBP genes (CvesOBP17/18/19) in female antennae demonstrate that they are likely involved in parasitic processes of female wasps, as the wasps take a longer time to target the hosts when they are knocked down.
- Subjects :
- Male
media_common.quotation_subject
Wasps
Insect
Vestalis
Moths
Receptors, Odorant
General Biochemistry, Genetics and Molecular Biology
RNA interference
medicine
Animals
Cotesia vestalis
Gene
Phylogeny
Ecology, Evolution, Behavior and Systematics
media_common
Genetics
Olfactory receptor
Diamondback moth
biology
fungi
biology.organism_classification
medicine.anatomical_structure
Larva
Insect Science
Odorant-binding protein
biology.protein
Insect Proteins
Female
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 17447917 and 16729609
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Insect Science
- Accession number :
- edsair.doi.dedup.....31812ac9446ead69fc077d84c6e6d96b