Search

Umbilical vein catheterization (UVC) is a common operation for vascular access in preterm infants. However, there are complications associated with their use. We here a case of extravasation of the fluids due to misplacement of the catheter causing hepatic collection of TPN in a very low-birth weight preterm (VLBW) infant.

Hen eggs (HEs) provide valuable nutrients for humans, including proteins, carbohydrates, lipids and vitamins. Recent studies revealed a number of novel egg-derived proteins/peptides (EDPs), and EDPs may play a crucial role in food industry and medical therapy. First, these EDPs were purified from the enzyme-catalyzed hydrolysates of egg proteins and were characterized by biochemical assays such as gel electrophoresis, HPLC, mass spectrometry, proteomic and peptideomic analysis, etc. Second, some EDPs can be used as nontoxic bio-preservatives and functional nutraceuticals for replacing harmful sodium nitrite, inhibiting foodborne pathogens, promoting metal-ion absorption and improving meat-product quality, and these new features will be widely used in the field of food production. Third, novel medical properties of EDPs comprise anti-oxidative, anti-microbial, anti-inflammatory and anti-nociceptive activities, which will benefit prevention of cardiovascular diseases, cancers, diabetic mellitus, immune disorders, etc. In summary, this review gives a real insight into the novel nutritional, biological and medical functions of EDPs, predictably facilitating the applications of EDPs in production of nutritive supplements, functional nutraceuticals and therapeutic medicines.

Coacervation of the lipase from Aspergillus oryzae (AOL) with chitosan was a feasible way to fabricate lipase-loaded particles and the optimum conditions were phase separation pH 5.5, chitosan to AOL mass ratio 1:5, and temperature 25 °C in the absence of NaCl, which conferred an AOL loading efficiency of up to 95.48% and activity recovery of 69.60%. The AOL-chitosan coacervates were highly porous and more susceptible to weight loss upon heating. Coacervation with chitosan increased the activity of AOL and shifted its optimum pH from 7.0 to 6.0, but exerted no effect on its optimum temperature (45 °C). Thermal deactivation kinetics analysis revealed that the coacervated AOL was more thermal stable, while the Michaelis-Menten kinetics analysis indicated that coacervation with chitosan increased the V

We report here the synthesis, characterization of seventeen (E)-2-methyl-3-ferrocenyl-N-acrylamides. The single crystal structures of the key intermediate b and two target compounds d5 and d6 were confirmed by X-ray crystallographic analysis. Antitumor activity of all compounds was tested in vitro. Compound d1 was the most significant one against B16 F10 cell line, indicating that it could be a good candidate for further study. Furthermore, cancer cell apoptosis assay was performed and the result indicated that compound d1 effectively fueled B16 F10 cells apoptosis in a dose-dependent manner.

Many industrial wireless sensor networks deploy the IEEE 802.15.4-2015 standard with low power consumption and the Internet networking capabilities. The time-slotted channel hopping (TSCH) is an amendment to the medium access control protocol defined by the IEEE 802.15.4 standard. Furthermore, the Internet engineering task force (IETF) defines an architecture for IPv6 over the TSCH mode of IEEE 802.15.4 (6TiSCH) to provide deterministic properties on wireless sensor networks. The TSCH leverages the scheduled time slot to achieve low power operation and frequency hopping to combat external interference and multi-path fading. Although TSCH provides the channel hopping mechanism to increase transmission reliability, it is still susceptible to periodic interference for the specific frequency. In this research, we focus on the issue of random channel hopping in the error-prone wireless channel condition and the scheduling for different service requirements. We propose a priority-based scheduling using best channel (P-SBC) mechanism for periodic sensing data and emergency packets transmission in 6TiSCH networks. The simulation results show that the proposed P-SBC achieves higher reliability with significantly lower end-to-end latency for emergency packets transmission as compared to TSCH standard.

Idiopathic pulmonary fibrosis is known as a life-threatening disease with high mortality and limited therapeutic strategies. In addition, the molecular mechanism by which pulmonary fibrosis developed is not fully understood. Asiatic acid (AA) is a triterpenoid, isolated from Centella asiatica, exhibiting efficient anti-inflammatory and anti-oxidative activities. In our study, we attempted to explore the effect of Asiatic acid on bleomycin (BLM)-induced pulmonary fibrosis in mice. The findings indicated that pre-treatment with Asiatic acid inhibited BLM-induced lung injury and fibrosis progression in mice. Further, Asiatic acid down-regulates inflammatory cells infiltration in bronchoalveolar lavage fluid (BALF) and pro-inflammatory cytokines expression in lung tissue specimens induced by BLM. Also, Asiatic acid apparently suppressed transforming growth factor-beta 1 (TGF-β1) expression in tissues of lung, accompanied with Collagen I, Collagen III, α-SMA and matrix metalloproteinase (TIMP)-1 decreasing, as well as Smads and ERK1/2 inactivation. Of note, Asiatic acid reduces NOD-like receptor, pyrin domain containing-3 (NLRP3) inflammasome. The findings indicated that Asiatic acid might be an effective candidate for pulmonary fibrosis and inflammation treatment.

Objective A novel genetic and molecular basis of nonalcoholic fatty liver disease (NAFLD) was explored. Study design A 38-year-old male, who has no bad living and dietary habits, was diagnosed as NAFLD. The potential pathogenic role of Pin1 was evaluated by enzyme-linked immunosorbent (ELISA) assay and single nucleotide polymorphism (SNP) sequencing. Results ELISA determined a six-time higher concentration of plasma Pin1 compared to our previous data. Nine PIN1 SNPs were sequenced and classified according to their NAFLD-pathogenic risks, suggesting that rs2233678 and rs2287839 may be the most important genotypes that result in Pin1 overexpression and NAFLD development. Conclusion In summary, this work explores a novel basis for early-onset NAFLD and highlights that elevated plasma Pin1 may predict NAFLD risk at early stage. Hypothetically, inhibiting Pin1 may benefit NAFLD prevention in the future., Highlights • We explored a novel genetic and molecular basis for a young NAFLD patient. • The individual had an obviously high concentration of Pin1 in blood plasma. • Nine PIN1 SNPs were measured and their NAFLD-pathogenic risks were predicted. • Pin1's clinical values will lie in early-diagnosis and precision treatment of NAFLD.

According to recent studies, food-borne microorganisms are closely related to human diseases, and dysbiosis of the intestinal microbiome was found to be a common pathogenic mechanism underlying several kinds of human diseases such as cancer, cardiovascular disease, inflammatory bowel disease, neural deficiency, etc

In a field experiment with three levels of irrigation, i.e., CI, DI通过大田试验研究了灌水量分别为充分灌水的100%(CI)、75% (DI

The red loam had abundant rainfall, rich in light and heat resource of Yunnan Province. But the uneven distribution of rainfall temporal during the year, it restricts the development of agriculture in this region. Thus how to use soil water resources reasonably and efficiency become the key to ensure crop and vegetation growth of needed water and improve the ecological environment. In this study, the key factor is district’s slope. Setting the runoff district’s rainfall intensity was 28.7mm/24h under rainfall simulation, selected four Yunnan Province read loam’ slop: 7.5°、10°、12.5°、15°.The results showed that at the same soil prophase water content and rainfall intensity, the higher runoff district’s slope will make it less time to product flow; At the beginning of product flow, flow of underground runoff was little, product flow was reached stable increase.

The recent IEEE 802.15.4-2015 standard proposes a number of Medium Access Control (MAC) layer protocols for low-power and lossy networks. The Timeslotted Channel Hopping (TSCH) is one of the proposed MAC operation modes, which take the advantage of channel hopping to make the transmissions more reliable and deterministic. In TSCH, slotframe is sliced into a timeslots and random channel hopping is used to mitigate the effects of co-channel interference and multipath fading. In this paper, we focus on the issue of random channel hopping in the error-prone wireless channel condition. In this research, a QoS Channel Allocation Scheme (QCAS) is proposed to enhance the reliability of emergency packet transmission in 6TiSCH networks. Based on our simulation results, we have observed that the proposed QCAS can achieve higher reliability of transmission over selected channel to provide higher throughput and higher delivery ratio than the original TSCH.

Studies of gene rearrangements and the consequent oncogenic fusion proteins have laid the foundation for targeted cancer therapy. To identify oncogenic fusions associated with glioma progression, we catalogued fusion transcripts by RNA-seq of 272 gliomas. Fusion transcripts were more frequently found in high-grade gliomas, in the classical subtype of gliomas, and in gliomas treated with radiation/temozolomide. Sixty-seven in-frame fusion transcripts were identified, including three recurrent fusion transcripts: FGFR3-TACC3, RNF213-SLC26A11, and PTPRZ1-MET (ZM). Interestingly, the ZM fusion was found only in grade III astrocytomas (1/13; 7.7%) or secondary GBMs (sGBMs, 3/20; 15.0%). In an independent cohort of sGBMs, the ZM fusion was found in three of 20 (15%) specimens. Genomic analysis revealed that the fusion arose from translocation events involving introns 3 or 8 of PTPRZ and intron 1 of MET. ZM fusion transcripts were found in GBMs irrespective of isocitrate dehydrogenase 1 (IDH1) mutation status. sGBMs harboring ZM fusion showed higher expression of genes required for PIK3CA signaling and lowered expression of genes that suppressed RB1 or TP53 function. Expression of the ZM fusion was mutually exclusive with EGFR overexpression in sGBMs. Exogenous expression of the ZM fusion in the U87MG glioblastoma line enhanced cell migration and invasion. Clinically, patients afflicted with ZM fusion harboring glioblastomas survived poorly relative to those afflicted with non-ZM-harboring sGBMs (P < 0.001). Our study profiles the shifting RNA landscape of gliomas during progression and reveled ZM as a novel, recurrent fusion transcript in sGBMs.

Bombyx mori cathepsin B (BmCatB) is involved in the programmed cell death of the fat body during B. mori metamorphosis. For a better understanding of the functional regulatory mechanism, the promoter region of BmCatB in the transcriptional regulation has been identified and analyzed in the present study. BmCatB promoter region performed by the 5' truncation or mutagenesis of EcREs was inserted in the pFA3Luc-A3RL double fluorescence expression vector to activate the fireflies luciferase (FLuc) gene. The results indicated that the dual-luciferase activity of BmCatB gene in the silkworm larval fat body is regulated by the length of promoter. Site-directed mutagenesis of EcRE experiment has shown that the EcREs are up-regulated significantly in the regulation of the BmCatB promoter. A 142bp region (-1165 to -1023) and EcREs are the mainly fat-body tissue-specificity related region and could function as a core promoter element.

In our Study, we Find that Location-Assisted on-Demand Routing (LAOR) Algorithm has Redundancy Control Overhead. Furthermore, LAOR does Not make Full Use of the Effective Information of New Path to Complete the Follow-up Path. to Address these Issues, an Improved Effective on-Demand Routing, Effective Improved on-Demand Routing (EIOR) Algorithm, is Proposed in this Paper. EIOR Makes Full Use of the Information of Control Packet to Update more Routing Paths, Reduces the Control Overhead for Finding Path and Increases the Real Time of Routing Updates. Moreover, EIOR Increases the Probability of Intermediate Satellite Replies Request Instead of the Destination Satellite, which Shortens the Setup Time of Path and Reduces the End-to-End Delay. it also Designs the RREP Immune Mechanism to Reduce the Control Overhead of the Network. Theoretical Analysis and Simulation Results Show that EIOR Outperforms LAOR. the EIOR can Reduce at Least 7% of the Normalized Control Overhead and 4% of the Average End-to-End Delay.

While screening for cellulase-producing fungi from insect gut, a fungus with high endoglucanase (carboxymethyl cellulase; CMCase) activity was isolated from the larval gut of Bombyx mori. Based on morphological characteristics and using an 18S rRNA-based molecular phylogenetic approach, the fungus, strain BMC-2, was identified as a Mucor sp. expressing a novel alkalotolerant cellulase. The maximum production of cellulase by the BMC-2 strain was observed at 55 °C and pH 8.0. The CMCase activity was inhibited by Cu2 + > Na+ > Zn2 + > Mg2 + > Ba2 +, and induced by Ca2 +, Mn2 +, Fe2 +, and K+.

The gene encoding cathepsin D of silkworm, Bombyx mori (BmCatD) is specifically expressed in the larval fat body and pupal gut, and plays an important role in the programmed cell death during metamorphosis. To identify element involved in this transcription-dependent spatial restriction, truncation and deletion of the 5' terminal from the BmCatD promoter were conducted in vivo. The recombinant AcMNPV vector (Autographa californica multiple nucleopolyhedrovirus) with a dual-luciferase quantitative assay system was used as the transfer. A 289 bp DNA sequence (-1,214 to -925) upstream of the transcriptional start site is found to be responsible for promoting tissue-specific transcription. Further analysis of a series of deletion within the 289 bp region of overlapping deletion showed that a 33 bp region (-1,071 to -1,038) sequence suppresses the ectopic expression of the BmCatD promoter. These results suggest that this 33 bp region could function as a promoter element in the tissue-specificity expression.

Natural Leymus Chinensis evapotranspiration in the Otindag sandy area under shallow ground-water level condition is calculated based on Soil-Water-Atmosphere-Plant (SWAP) Model developed by Wageningen Agricultural College. The observed values in open test and interior experiment, the meteorological observation and crop index, and the data of soil volumetric moisture content in 2005, are used to calibrate parameters of SWAP model, determine and check out the water transfer parameters of SWAP model with soil volumetric moisture content in 2006. The result shows that SWAP model has high precision and can be use to simulate evapotranspiration under shallow groundwater level condition.

In opportunistic networks, routing algorithms based on community division have some redundant forwarding in the process of message transmission. In this paper, we propose a community-based message transmission algorithm (CMTA). CMTA makes fully use of the nodes within communication overlapping areas to forward messages and optimizes forwarding determination mechanism to carry message nodes, then CMTA redesigns the message transmission condition and reduces the number of message forwarding which improve the efficiency of forwarding messages and reduce the average end-end delivery delay. Theoretical analysis and simulation results show that CMTA outperforms an existing Epidemic routing algorithm and the Prophet algorithm and CHMTS in terms of delivery ratio, average end-end delivery delay, relay ratio, etc.

The worn Cu-based friction disks were repaired and remanufactured by the spark plasma sintering (SPS) technology. The morphology and component of the repaired friction disk were analysed by SEM and EDS. The mechanical properties were characterized by microhardnessmeter and friction tester. The results indicated that the microstructure of the repaired areas combined closely with the worn areas and the components were well-distributed. The friction coefficient of the repaired materials is close to the unrepaired, but the hardness declined slightly.

La0.6Sr0.4Co0.2Fe0.8O3-δ composite oxide was prepared and characterized. Dilatometer and four-probe DC were exploited to investigate the thermal expansion and electrical conductivity, respectively. The thermal expansion curve was linear, but it became steeper at the high temperature region, as a result of the loss of lattice oxygen and the formation of oxygen vacancies. The conductivity increased with temperature up to about 600oC, and then decreased due to the loss of lattice oxygen. The maximum conductivity was more than 300 S cm-1. The chemical diffusion coefficient in La0.6Sr0.4Co0.2Fe0.8O3-δ was estimated by analyzing the conductivity relaxation behavior. The relaxation process of the conductivity change for La0.6Sr0.4Co0.2Fe0.8O3-δ was traced as a function of time, at a fixed temperature. It was found that the chemical diffusion coefficients measured at temperatures 720-770oC vary from 710-8 to 110-7 cm2 S-1. The activation energy for oxygen diffusion in La0.6Sr0.4Co0.2Fe0.8O3-δ, derived from the chemical diffusion coefficient is 40.8±3.6 kJ mol-1.

In order to improve simulation precision of saturated hydraulic conductivity, use RETC and PTFs function to simulate saturated hydraulic conductivity by measured research area surface soil (0-20cm) physicochemical property and soil texture data. The simulation results were assessed by the approach of consisting in presenting a graphical representation of model simulated compared with observed values and by mean square error. The main results indicate that the RETC-PTFs and local PTFs mean square errors are 1.19 and 0.40 respectively. The precision of RETC-PTFs is lower than local PTFs function obviously using soil physicochemical property and soil texture data. The RETC-PTFs is inappropriate to this area. Accuracy of each input data precision of RETC-PTFs play a big part in because of less data needed. More complete data not always get better simulating results.

The research aims to develop inorganic thermal-insulating materials of high performance, decrease high geothermal emissions from hot rock to the roadway, reduce the roadway airflow temperature, and achieve mine cooling and energy saving eventually. Firstly, the best proportion of inorganic thermal-insulating materials of high performance was designed, and a test model for thermal conductivity and mechanical analysis has been built. And through the test of thermal-insulating of the material and mechanical properties, the relation equations between vitreous micro-bead content (the main agent of thermal-insulating material) and thermal conductivity, uniaxial compressive strength, confined compressive strength have been obtained respectively. Secondly, the feature, the fitting and coupling features between the experimental models and engineering conditions in practice were analyzed comparatively, which could provide theoretical basis for the design and application of thermal-insulating materials of high performance. The industrial test shows that with thermal-insulating materials of high performance in application, the roadway airflow temperature was reduced significantly and could meet the requirements of mine pressure. Therefore, the research could offer an effective way for the mine thermal-insulating and cooling under high geothermal conditions.

According to the questions of long eliminating coal and gas outburst period, large projects and slow driving came from the regional measurement of drilling hole ,the paper select 2371 (1) rail transport roadway as experiment for using along seam long drilling as a regional measurement to eliminate coal and gas outburst. Since the regional measurement of along seam long drilling had been carried out, the remaining gas content decreased more than 60％, max Smax was 5.4kg/m, max qmax was 2.01/min.Both Smax and qmax were not beyond the standard. Gas concentrations were not overrun. Gas emission decreased in the roadway afterward. The regional measurement eliminated the risk of coal and gas outburst in effect. Heading face promoted 423m forward safely. The max January footage could reach 120m.Achieve driving of heading face safely and fast.

Roadway insulation and active cooling supporting structure is a complex system, and the mechanical properties play an important roal in its safety. Analyse the shotcrete layer pattern heat insulation structure by Midas-GTS software, calculation results shows that tensile stress zone appears in the top and bottom of heat insulation layer, while the will compressive stress area appears in two sides of the roadway insulation layer; in the case of 500m buried depth, heat insulation layer material, such as vitreous micro-bead, can well meet the engineering mechanical strength requirements.

Working principle of an uninterrupted shift transmission (UST) based on overrunning mechanism and its driveline dynamic model, as well as shift control strategy are studied. For shift quality optimization, the throttle control parameters are calculated based on simplex method and genetic algorithm. Using the control strategy and optimized parameters, simulation results show that jerks and clutch slip during shift are controlled well, satisfactory shift quality is obtained without power flow interruption.

In view of the measurement difficulties of indexes recommended by the 50 Items Experience of Coal Mine Gas Prevention in the process of the nonoutburst coal seam upgrade, this paper took the No.8 coal seam of Huainan Mining Group as research object. Discussed the suitability of indexes and corresponding critical values, put forward method in determining the indexes and its critical values by analysis and investigation of the gas geological condition and the-spot tracking near position where an outburst occurred combined with laboratory experiment, and established the indexes and its critical values of nonoutburst coal seam upgrade in No.8 coal seam of Huainan Mining Group. The results show that it is suitable to take gas content and tectonic soft coal thickness easily to gain in routine production as primary upgrade indexes that its critical values are 7.5 m3/t and 0.8 m, respectively. In addition, take f value and Δp value as auxiliary indexes.

Myocardial ischaemia/reperfusion (MI/R) injury is characterized by metabolic and ultrastructural changes, which lead to irreversible injury. Several mechanisms have been postulated for the pathogenesis of MI/R injury although little is known regarding the role of myocardial gene expression.In this study, suppression subtractive hybridization (SSH) was employed to systematically isolate and screen the differentially expressed genes in the MI/R injury rat model. The characteristics of these specific genes were analysed using bioinformatics. Our results showed that among the 119 identified genes, 54 genes were expressed at higher levels and 65 genes were at lower levels compared with the control group.These genes are closely associated with energy metabolism, iron transport, signalling transduction and may provide important clues for the elucidation of the mechanisms of MI/R injury. Our results further indicated that myocardial injury is likely the result of summation of functional impairment of multiple genes rather than the result of damage to a single critical gene.

Jatropha curcas is the most promising energy tree, and soil moisture is the key factor which affects the seedling quality and water use efficiency of J. curcas. With aims to evaluate the effect of different irrigation amount on growth, morphological characteristics and water use of J. curcas, a pot experiment was conducted with four irrigation amounts, i. e., W1:472.49 mm, W2: 228.79 mm, W3:154.18 mm and W4:106.93 mm, respectively. Compared with W1 treatment, the leaf area and stem cross-section area of base significantly decreased in W2, W3 and W4 treatments, but Huber value significantly increased, which could improve the efficiency of water transfer from root to shoot, thus enhance the capability of resistance to drought stress. Compared with W, treatment, the healthy index of J. curcas seedlings decreased slightly in W2 treatment but significantly decreased in W3 and W4 treatments. Hence, the irrigation amount from 228.79 to 472.49 mm was beneficial to increase the healthy index of J. curcas seedlings. Compared with W1 treatment, irrigation water was saved by 67.4% in W3 treatment, and the total dry mass and evapotranspiration significantly decreased by 17.4% and 68.6%, and the irrigation water use efficiency and total water use efficiency increased by 153.2% and 163.2%, respectively. In the condition of this study, the irrigation amount of 154.18 mm was beneficial to increase water use efficiency.