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One of the central issues in cognition is identifying universal and culturally specific patterns of thought. In this study, we examined how one aspect of culture, a linguistic part of speech known asclassifiers, are related to categorization of solid objects. In Experiment 1, we used a numeral classifier elicitation task to examine the classifiers used by speakers of Hmong, Japanese, and Mandarin Chinese (N = 34) with 135 nouns that referred to solid objects. In Experiment 2, adult speakers of English, Japanese, Mandarin Chinese, and Hmong (N = 64) rated the similarity of 39 pictured objects that depicted a subset of the nouns. All groups classified the objects into natural kinds and artifacts, with the category of humans anchoring both divisions. The main difference that emerged from the study was that speakers of Japanese and English rated humans and animals as more similar to each other than Hmong speakers; Mandarin speakers' ratings of the similarity between humans and animals fell in between those of Hmong and English speakers. However, the pattern of categorization of humans and animals found among speakers of the classifier languages contradicted their patterns of classifier use. The findings help to tease apart the effects of language from other cultural factors that impact cognition.

Purpose. To investigate changes in corneal densitometry (CD) and visual quality following small incision lenticule extraction (SMILE) and laser epithelial keratomileusis (LASEK) in patients with mild-to-moderate myopia. Methods. A retrospective analysis was performed on 24 and 25 patients (46 eyes each) who underwent SMILE and LASEK, respectively, for mild-to-moderate myopia. The visual quality and CD values were recorded. Using the Pentacam Scheimpflug system, CD values were collected in three concentric optical zones at the depths of the anterior, central, and posterior layers. Efficacy, safety, predictability, corneal wavefront aberrations, and QoV scores were measured to evaluate visual quality. A correlation analysis was performed between changes in CD and clinical characteristics. Results. There were no statistical differences in efficacy and safety indices between the two groups. At 3 months postoperatively, a pronounced reduction in several zones was observed in the LASEK group ( p < 0.05 ), whereas no obvious change was observed in the SMILE group. There were obvious changes in the CD values in several zones in the SMILE and LASEK groups ( p < 0.05 ) after 1 year. The magnitude of the CD changes in the anterior and central corneal layers was smaller in the SMILE group than in the LASEK group (all p < 0.05 ). Lower HOAs, spherical aberration, and horizontal comas of the anterior and whole corneal surfaces were observed in the SMILE group. QoV scores were similar between the two groups. Conclusion. CD decreased in the SMILE and LASEK groups after 1 year; there was a smaller reduction in SMILE than in LASEK. SMILE and LASEK did not differ significantly in terms of safety and effectiveness in correcting mild-to-moderate myopia.

Aim: We aimed to establish and validate a simple and sensitive UPLC–MS/MS method for the determination of UNC1999, a dual inhibitor against EZH1 and EZH2 in plasma samples. Materials & methods: UNC1999 in rat plasma was processed with protein precipitation method and then separated on a C18column and detected under positive ionization mode. The method presented good linearity over the range of 1.0–2000 ng/ml with good accuracy and precision. UNC1999 was absorbed slowly and achieved a maximum concentration of 118.8 ± 12.0 ng/ml 1.5 h after oral administration. Conclusion: The method provides a favorable character in selectivity, linearity, accuracy, precision, recovery, matrix effects and stabilities and was suitable for describing the pharmacokinetic profile of UNC1999.

The demand for in-situ detection of latent fingerprints (LFPs) in ways of high sensitivity, high selectivity, high contrast, low cost and user-friendly is still urgent. To overcome this challenge, a moisture-stable, red-emitting fluoride phosphor K3AlF6:Mn4+ (KAF:Mn4+) with an organic hydrophobic skin was prepared. The phosphor has a uniform and superfine morphology with excellent luminescence properties. More importantly, this non-ultraviolet (UV) or non-near infrared (NIR) induced phosphor was proved to be an ideal fluorescent label for LFP imaging, which is both friendly for touch DNA analysis and compatible to forensic light sources. The well-defined ridge details with little background interference on various surfaces were presented by the oleic acid (OA) modified KAF:Mn4+ (KAF:Mn4+-OA) phosphor in few seconds using the powder dusting method. To confirm the high selectivity of KAF:Mn4+-OA for LFP imaging, an efficient quantitative evaluation method is proposed with the aid of ImageJ & Origin software. Due to the superiority of the Mn4+-doped fluoride for the rapid imaging of LFPs in terms of low-cost, high compatibility and good availability, it is expected to be a promising candidate for forensic science as well as fluorescence imaging in other fields instead of rare earth luminescent materials.

Correlations in gene expression are used to infer functional and regulatory relationships between genes. However, correlations are often calculated across different cell types or perturbations, causing genes with unrelated functions to be correlated. Here, we demonstrate that correlated modules can be better captured by measuring correlations of steady-state gene expression fluctuations in single cells. We report a high-precision single-cell RNA-seq method called MALBAC-DT to measure the correlation between any pair of genes in a homogenous cell population. Using this method, we were able to identify numerous cell-type specific and functionally enriched correlated gene modules. We confirmed through knockdown that a module enriched for p53 signaling predicted p53 regulatory targets more accurately than a consensus of ChIP-seq studies and that steady-state correlations were predictive of transcriptome-wide response patterns to perturbations. This approach provides a powerful way to advance our functional understanding of the genome.

Dry eye disease (DED) is the most common disease affecting vision and quality of life. PM2.5 was a potential risk of DED. Herein, we conducted animal exposure and cell-based studies to evaluate the pathogenic effect of PM2.5 exposure on the ocular surface and DED etiological mechanisms. C57 mice were exposed to filtered air and PM2.5 aerosol. We assessed health conditions and inflammation of the ocular surface by corneal fluorescein staining and immunohistochemistry. In parallel, cultured human corneal epithelial cells (HCETs) were treated with PM2.5, followed by characterization of cell viability, intracellular ATP level, mitochondrial activities, and expression level of DED relevant mRNA and proteins. In mice, PM2.5 exposure induced severe superficial punctate keratopathy and inflammation in their cornea. In HCETs, cell proliferation and ROS generation followed dose-response and time-dependent manner; meanwhile, mitochondrial ROS (mtROS) level increased and mitochondrial membrane potential (MMP) level decreased. Inflammation cascade was triggered even after short-term exposure. The reduction of ATP production was alleviated with Nrf2 overexpression, NF-κB P65 knockdown, or ROS clearance. Nrf2 overexpression and P65 knockdown reduced inflammatory reaction through decreasing expression of P65 and increasing of Nrf2, respectively. They partly alleviated changes of ROS/mtROS/MMP. This research proved that PM2.5 would cause DED-related inflammation reaction on corneal epithelial cells and further explored its mechanism: ROS from mitochondrial dysfunctions of corneal epithelial cells after PM2.5 exposure partly inhibited the expression of anti-inflammatory protein Nrf2 led the activation of inflammatory protein P65 and its downstream molecules, which finally caused inflammation reaction. Graphical abstract

Proliferative vitreoretinopathy (PVR) is an intractable condition after rhegmatogenous retinal detachment (RD), which is the primary cause of failure in retinal reattachment surgery. This study aimed to investigate the effects of chicken ovalbumin upstream promoter transcriptional factor 1 (COUP-TF1) in the development of proliferative vitreoretinopathy (PVR) both in vitro and in vivo. Adult retinal pigment epithelium cell line was used for in-vitro experiments. Immunocytochemistry assay, real-time quantitative polymerase chain reaction, and Western blot were used to measure the expression of COUP-TF1, alpha-smooth muscle actin (α-SMA), and E-cadherin. Epithelial-mesenchymal transition (EMT) was observed through cell counting kit-8 assay, wound healing tests, and the expression changes of related proteins. PVR rabbit models were established and evaluated by the images of fundus and vitreous cavity, pathological sections, and COUP-TF1 expression. As shown by our results, the proliferation and migration of the COUP-TF1 knockdown cells were reduced compared with the control cells with or without transforming growth factor-β1 (TGF-β1) treatment. After TGF-β1 treatment, α-SMA expression was upregulated in ARPE-19 cells but kept the same in COUP-TF1 knockdown cells. E-cadherin expression was down-regulated in all the groups but the extent of the decrease in COUP-TF1 knockdown cells was smaller. EMT was attenuated in ARPE-19 cells after COUP-TF1 was knocked down. In the in-vivo experiment, PVR severity was attenuated and the retinal detachment rate decreased on the 14th and 28th day in COUP-TF1 knockdown group. In conclusion, COUP-TF1 is related to the development of PVR, and COUP-TF1 knockdown attenuates the progression of PVR. This suggests that COUP-TF1 can be a promising candidate for the treatment of PVR.

Stretching a coiled carbon nanotube (CNT) yarn can provide large, reversible electrochemical capacitance changes, which convert mechanical energy to electricity. Here, it is shown that the performance of these "twistron" harvesters can be increased by optimizing the alignment of precursor CNT forests, plastically stretching the precursor twisted yarn, applying much higher tensile loads during precoiling twist than for coiling, using electrothermal pulse annealing under tension, and incorporating reduced graphene oxide nanoplates. The peak output power for a 1 and a 30 Hz sinusoidal deformation are 0.73 and 3.19 kW kg

Senescent cells have been demonstrated to have lower cellular NAD+ levels and are involved in the development of various age-related diseases, including age-related macular degeneration (AMD). Sodium iodate (NaIO3) has been primarily used as an oxidant to establish a model of dry AMD. Results of previous studies have showed that NaIO3 induced retinal tissue senescence in vivo. However, the role of NaIO3 and the mechanism by which it induces retinal pigment epithelium (RPE) senescence remains unknown. In this study, RPE cell senescence was confirmed to be potentially induced by NaIO3. The results showed that the number of senescence-associated-β-galactosidase (SA-β-gal-)-positive cells and the protein levels of p16 and p21 increased after NaIO3 treatment. Additionally, the senescent RPE cells underwent oxidative stress and NAD+ depletion. Furthermore, significant DNA damage and mitochondrial dysfunction were also detected in senescent RPE cells. The antioxidant N-acetylcysteine (NAC) could alleviate cellular senescence only by a minimal degree, whereas supplementation with nicotinamide mononucleotide (NMN) strongly ameliorated RPE senescence through the alleviation of DNA damage and the maintenance of mitochondrial function. The protective effects of NMN were demonstrated to rely on undisturbed Sirt1 signaling. Moreover, both the expression of senescence markers of RPE and subretinal inflammatory cell infiltration were decreased by NMN treatment in vivo. Our results indicate that RPE senescence induced by NaIO3 acquired several key features of AMD. More importantly, NMN may potentially be used to treat RPE senescence and senescence-associated pre-AMD changes by restoring the NAD+ levels in cells and tissues.

Aim. Chronic inflammation is crucial for age-related macular degeneration (AMD) pathogenesis. However, the mechanism involved in activating inflammation remains unclear. This study is aimed at investigating whether nuclear factor erythrocyte-associated factor 2 (Nrf2) negatively regulated the Nod-like receptor protein 3 (NLRP3) inflammasomes through the thioredoxin 1 (Trx1)/thioredoxin interaction protein (TXNIP) complex. Methods. We determined the optimal hydrogen peroxide (H2O2) concentration, time, and changes in reactive oxygen species (ROS) levels. We also constructed animal models using blue LED irradiation. Then, the expression of Nrf2, TXNIP, Trx1, NLRP3, and inflammation-related factors and proteins, along with the changes in retinal thickness and functional status, was analyzed. Results. The oxidative stress model was established after 1 h intervention with 100 μM H2O2. Nrf2 reduced ROS production, protected the ultrastructure of mitochondria, increased the thickness of the ONL layer, and increased the amplitude of a- and b-wave amplitudes in ERG. Trx1 knockdown increased the production of ROS, damaged the ultrastructure of mitochondria, reduced the thickness of the other ONL layer, and reduced the amplitudes of a- and b-waves in the electroretinogram (ERG). Thus, TXNIP in the cytoplasm activated the inflammasomes. Conclusions. Nrf2 showed antioxidant and anti-inflammatory activity in the H2O2-induced cell stress model and blue LED-induced retinal light damage model. TXNIP transferred from the nucleus to the cytoplasm, activated NLRP3, and aggravated the retinal injury in both the cell stress model and the animal blue LED model. In contrast, Trx1 knockout promoted this process. This study revealed the possible role of the thioredoxin system in developing AMD while also providing newer insights for the future treatment of AMD.

The isolation and structural characterization of three new monometallic uranium metallofullerenes, U@D2(21)-C84, U@Cs(15)-C86, and U@C1(11)-C86, allowed us to complete an interconversion map for al...

An oxyfluoride red phosphor Cs2MoO2F4:Mn4+ was synthesized via a facile co-precipitation route with a certain molecular ratio of CsF and MoO3. X-ray diffraction analysis and its Rietveld refinement reveal that Cs2MoO2F4:Mn4+ crystallized in an orthorhombic structure with the Amam (63) space group. Upon blue light excitation, Cs2MoO2F4:Mn4+ exhibits a series of sharp red emission lines around ∼634 nm and the zero-phonon line (ZPL) is visible at 619 nm. The optimal doping amount of Mn4+ in Cs2MoO2F4 is 1.12%, and the decay curves show a good fit with the single exponential decay model. The fluorescence lifetime of the synthesized phosphors is relatively short and calculated as 3.18 to 2.46 ms, the Mn4+ ions in Cs2MoO2F4 experience a strong crystal field strength with a Dq/B of ∼4.87, and the distinct nephelauxetic ratio β1 is determined to be ∼1.0226. The thermal quenching mechanism of Mn4+ was also studied. Furthermore, by using the as-synthesized Cs2MoO2F4:Mn4+ phosphor as a red component and β-SiALON as a green light component, a WLED was fabricated with a high luminous efficacy of 114.70 lm·W−1 and wide color gamut of 109.1% of the National Television Standard Committee (NTSC) value. Hence, the Cs2MoO2F4:Mn4+ phosphor with a short fluorescence lifetime could potentially be an efficient red compensator for application in rapid-response backlight displays.

The crucial effect of vascular endothelial growth factor (VEGF)-induced vascular angiogenesis has been well known in corneal neovascularization (CNV). This research aimed to determine the underlying value and mechanism of Meg3 on CNV in vivo and in vitro. In an alkali-burned mouse model, length and area of new vessels were increased along with thinning of corneal epithelium, accompanied by the overexpression of Meg3. Notably, subconjunctival injection of shMeg3 suppressed the degree of injury in cornea, causing expression of the angiogenesis markers--VEGF-A and CD31 decreased. In VEGF-induced human umbilical vein endothelial cells (HUVECs), knockdown of Meg3 antagonized the enhancement of viability, proliferation, wound healing ability and angiogenesis by VEGF. The proteins expression of VEGF-A, CD31, SDF-1/CXCR4 as well as phosphoraylation-Smad2/3 pathways, which were related to angiogenesis, were reduced with Meg3 deficiency. Overall, knockdown of Meg3 alleviated formation of neovascularization in alkali-burned corneas and reduced VEGF-induced angiogenesis by inhibiting SDF-1/CXCR4 and Smad2/3 signaling in vitro.

Deterioration of the ecological environment in the upper and middle reaches of the Yellow River in China substantially impacts the growth and development of aquatic organisms in the drainage basin. This paper builds a conceptual model by applying flow components and fish ecological requirements relation with a relevant object of main fish in the upper and middle reaches of the Yellow River. The paper utilized the flow restoration method by employing the River2D model (two-dimensional model of river hydrodynamics and fish habitat), and a one-dimensional hydrodynamics HEC-RAS (hydrologic engineering center’s-river analysis system). The calculation result showed that the runoff condition required for Silurus lanzhouensis survival is that the monthly lowest flow in a year is 150 m3·s−1, and the lowest flow for suitable flow from April to October is 150 m3·s−1, and 300 m3·s−1 from November to March. The research result is closer to the actual condition and has more outstanding operability. Meanwhile, the results proposed the coupling method of ecological water requirement for the mainstream of the Yellow River. Moreover, the results portrayed the ecological flow process according to the upper envelope of minimum and maximum ecological water requirements of each fracture surface. It is regarded that the ecological flow process is deemed as the initial value of the reservoir regulation model.

Dy-based single-molecule magnets (SMMs) are of great interest due to their ability to exhibit very large thermal barriers to relaxation and therefore high blocking temperatures. One interesting line of investigation is Dy-encapsulating endohedral clusterfullerenes, in which a carbon cage protects magnetic Dy3+ ions against decoherence by environmental noise and allows for the stabilization of bonding and magnetic interactions that would be difficult to achieve in other molecular architectures. Recent studies of such materials have focused on clusters with two Dy atoms, since ferromagnetic exchange between Dy atoms is known to reduce the rate of magnetic relaxation via quantum tunneling. Here, two new dysprosium-containing mixed-metallic sulfide clusterfullerenes, DyScS@Cs(6)–C82 and DyScS@C3v(8)–C82, have been successfully synthesized, isolated and characterized by mass spectrometry, Vis-NIR, cyclic voltammetry, single crystal X-ray diffractometry, and magnetic measurements. Crystallographic analyses show that the conformation of the encapsulated cluster inside the fullerene cages is notably different than in the Dy2X@Cs(6)–C82 and Dy2X@C3v(8)–C82 (X = S, O) analogues. Remarkably, both isomers of DyScS@C82 show open magnetic hysteresis and slow magnetic relaxation, even at zero field. Their magnetic blocking temperatures are around 7.3 K, which are among the highest values reported for clusterfullerene SMMs. The SMM properties of DyScS@C82 far outperform those of the dilanthanide analogues Dy2S@C82, in contrast to the trend observed for carbide and nitride Dy clusterfullerenes., Extremely high magnetic blocking temperatures (∼7.3 K) were observed for DyScS endohedral fullerene single-molecule magnets.

Fullerene carbon cages can encapsulate a wide variety of atoms, ions, clusters, or small molecules inside, resulting in stable compounds with unusual structures and electronic properties. These compounds are collectively defined as endohedral fullerenes. The most studied endohedral fullerenes are those containing metal atoms or ions inside, and these are referred to as endohedral metallofullerenes (EMFs). For EMFs, the inner isolated space of the fullerene cages can lead to the stabilization of unique clusters, which are otherwise not synthetically accessible. This offers an excellent environment and opportunity for investigating the nature of previously unobserved metal-metal, metal-non-metal, and metal-fullerene interactions, which are of fundamental interest and importance. Up until now, most of the work in this field has been mainly focused on the rare-earth metals and related elements (groups II, III, and IV). The encapsulation of other elements of the periodic table could potentially lead to totally new structures and bonding motifs and to material properties beyond those of the existing EMFs. Actinides were originally explored as encapsulated elements in fullerenes when Smalley et al. ( Science 1992 , 257 , 1661 ) reported mass spectral evidence of actinide endohedral fullerenes back in 1992. However, the full characterization of these actinide endohedral fullerenes, including single crystal X-ray diffractometric analyses, was not reported until very recently, in 2017. In this Account, we highlight some recent advances made in the field of EMF compounds, focusing primarily on the molecular and electronic structures of novel actinide-based EMFs, new evidence for the formation mechanisms of EMFs, and the influence of the entrapped species on the reactivity and regiochemistry of EMF compounds. We recently reported that some monometallic actinide EMFs represent the first examples of tetravalent metals encapsulated inside fullerenes that exhibit considerably stronger host-guest interactions when compared to those observed for the lanthanide EMFs. These unusually strong metal-cage interactions, along with very high mobilities of the actinides inside the fullerene cages at high temperatures, result in the stabilization of unexpected non-IPR (isolated pentagon rule) fullerene cages encapsulating only one metal ion. Strikingly, such covalent stabilization factors had never been previously observed, although Sm@C2v(19138)-C76 was the first reported mono-EMF with a non-IPR cage, see details below. In addition, we showed that a long sought-after actinide-actinide bond was obtained upon encapsulation of U2 inside an Ih(7)-C80 fullerene cage. More interestingly, we demonstrated that actinide multiple bonds, which are very difficult to prepare by conventional synthetic methods, are stabilized when trapped inside fullerene cages. A totally unexpected and previously unreported uranium carbide cluster, U═C═U, was fully characterized inside an EMF, U2C@Ih(7)-C80, which, for the first time, clearly exhibits two unsupported axial U═C double bonds that are ∼2.03 A long. We also discovered that synthetic bis-porphyrin nanocapsules exhibit exquisitely selective complexation of some of these uranium endohedral compounds, providing the basis for a nonchromatographic EMF purification method for actinide EMFs. Regarding EMF formation mechanisms, we suggested that novel carbide EMF structures, that is, Sc2C2@Cs(hept)-C88, are likely key intermediates in a bottom-up fullerene growth process. Additionally, the structural correlation between chiral carbon cages during a bottom-up growth process was shown to be enantiomer-dependent. The influence of the encapsulated clusters on the chemical reactivity of EMFs is discussed at the end, which showed that the regioselectivities of multiple additions to the fullerene cages are remarkably controlled by the encapsulated metal clusters.

A novel actinide endohedral fullerene with an unexpected chiral cage, Th@C1(11)-C86, was synthesized and characterized. DFT calculations suggest that this low symmetry cage was favoured as a consequence of the strong interaction between Th and the cage, which makes the predictions by the ionic model less reliable for these endohedral mono-metallofullerenes.

For the first time, actinide endohedral metallofullerenes (EMFs) with non-isolated-pentagon-rule (non-IPR) carbon cages, U@C80, Th@C80, and U@C76, have been successfully synthesized and fully characterized by mass spectrometry, single crystal X-ray diffractometry, UV–vis–NIR and Raman spectroscopy, and cyclic voltammetry. Crystallographic analysis revealed that the U@C80 and Th@C80 share the same non-IPR cage of C1(28324)-C80, and U@C76 was assigned to non-IPR U@C1(17418)-C76. All of these cages are chiral and have never been reported before. Further structural analyses show that enantiomers of C1(17418)-C76 and C1(28324)-C80 share a significant continuous portion of the cage and are topologically connected by only two C2 insertions. DFT calculations show that the stabilization of these unique non-IPR fullerenes originates from a four-electron transfer, a significant degree of covalency, and the resulting strong host–guest interactions between the actinide ions and the fullerene cages. Moreover, because t...