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Development of a UPLC–MS/MS method for determination of a dual EZH1/2 inhibitor UNC1999 in rat plasma
- Source :
- Bioanalysis. 14:67-74
- Publication Year :
- 2022
- Publisher :
- Future Science Ltd, 2022.
-
Abstract
- Aim: We aimed to establish and validate a simple and sensitive UPLC–MS/MS method for the determination of UNC1999, a dual inhibitor against EZH1 and EZH2 in plasma samples. Materials & methods: UNC1999 in rat plasma was processed with protein precipitation method and then separated on a C18column and detected under positive ionization mode. The method presented good linearity over the range of 1.0–2000 ng/ml with good accuracy and precision. UNC1999 was absorbed slowly and achieved a maximum concentration of 118.8 ± 12.0 ng/ml 1.5 h after oral administration. Conclusion: The method provides a favorable character in selectivity, linearity, accuracy, precision, recovery, matrix effects and stabilities and was suitable for describing the pharmacokinetic profile of UNC1999.
- Subjects :
- Male
Indazoles
Chromatography
Plasma samples
Pyridones
Chemistry
Clinical Biochemistry
Dual inhibitor
General Medicine
High-performance liquid chromatography
Piperazines
Rats
Analytical Chemistry
Rats, Sprague-Dawley
Medical Laboratory Technology
Tandem Mass Spectrometry
Benzamides
Animals
Uplc ms ms
General Pharmacology, Toxicology and Pharmaceutics
Chromatography, High Pressure Liquid
Subjects
Details
- ISSN :
- 17576199 and 17576180
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Bioanalysis
- Accession number :
- edsair.doi.dedup.....0190333cf51845ad625dfc467684a397