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LncRNA Meg3 knockdown reduces corneal neovascularization and VEGF-induced vascular endothelial angiogenesis via SDF-1/CXCR4 and Smad2/3 pathway
- Source :
- Experimental eye research. 222
- Publication Year :
- 2021
-
Abstract
- The crucial effect of vascular endothelial growth factor (VEGF)-induced vascular angiogenesis has been well known in corneal neovascularization (CNV). This research aimed to determine the underlying value and mechanism of Meg3 on CNV in vivo and in vitro. In an alkali-burned mouse model, length and area of new vessels were increased along with thinning of corneal epithelium, accompanied by the overexpression of Meg3. Notably, subconjunctival injection of shMeg3 suppressed the degree of injury in cornea, causing expression of the angiogenesis markers--VEGF-A and CD31 decreased. In VEGF-induced human umbilical vein endothelial cells (HUVECs), knockdown of Meg3 antagonized the enhancement of viability, proliferation, wound healing ability and angiogenesis by VEGF. The proteins expression of VEGF-A, CD31, SDF-1/CXCR4 as well as phosphoraylation-Smad2/3 pathways, which were related to angiogenesis, were reduced with Meg3 deficiency. Overall, knockdown of Meg3 alleviated formation of neovascularization in alkali-burned corneas and reduced VEGF-induced angiogenesis by inhibiting SDF-1/CXCR4 and Smad2/3 signaling in vitro.
- Subjects :
- Receptors, CXCR4
Neovascularization, Pathologic
Vascular Endothelial Growth Factors
Neovascularization, Physiologic
Smad2 Protein
Alkalies
Sensory Systems
Cellular and Molecular Neuroscience
Ophthalmology
Eye Burns
Mice
Human Umbilical Vein Endothelial Cells
Animals
Humans
Corneal Neovascularization
RNA, Long Noncoding
Smad3 Protein
Corneal Injuries
Subjects
Details
- ISSN :
- 10960007
- Volume :
- 222
- Database :
- OpenAIRE
- Journal :
- Experimental eye research
- Accession number :
- edsair.doi.dedup.....dc443f669113e240e11fc5711291d791