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12. Yeast extract improves growth in rainbow trout (Oncorhynchus mykiss) fed a fishmeal-free diet and modulates the hepatic and distal intestine transcriptomic profile

Author

Frohn, Laura, primary, Peixoto, Diogo, additional, Guyomar, Cervin, additional, Teixeira, Carla, additional, Terrier, Frédéric, additional, Aguirre, Pierre, additional, Haddad, Sarah Maman, additional, Bobe, Julien, additional, Costas, Benjamin, additional, Richard, Nadège, additional, Pinel, Karine, additional, and Skiba-Cassy, Sandrine, additional

Published
2023
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14. Exploring the effects of dietary inulin in rainbow trout fed a high-starch, 100% plant-based diet

Author

Defaix, Raphaël, primary, Lokesh, Jep, additional, Frohn, Laura, additional, Bechec, Mickael Le, additional, Pigot, Thierry, additional, Véron, Vincent, additional, Surget, Anne, additional, Biasutti, Sandra, additional, Terrier, Frédéric, additional, Skiba-Cassy, Sandrine, additional, Roy, Jérôme, additional, Panserat, Stéphane, additional, and Ricaud, Karine Brugirard, additional

Published
2023
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15. Interaction between genetics and inulin affects host metabolism in rainbow trout fed a sustainable all plant-based diet.

Author

Lokesh, Jep, Delaygues, Marine, Defaix, Raphaël, Le Bechec, Mickael, Pigot, Thierry, Dupont-Nivet, Mathilde, Kerneis, Thierry, Labbé, Laurent, Goardon, Lionel, Terrier, Frédéric, Panserat, Stéphane, and Ricaud, Karine

Subjects
BIOLOGICAL models, GENETICS, GUT microbiome, ANIMAL experimentation, LIVER, PLANT-based diet, TREATMENT effectiveness, GENE expression, STREPTOCOCCUS, FISHES, DESCRIPTIVE statistics, INTESTINAL mucosa, INULIN, INTESTINES, ADIPOSE tissues
Abstract

Inulin affects nutrition and metabolism in many animals. Although inulin is widely used in the diet of teleosts, its mechanism of action is unknown. Here, we investigated the effect of inulin (2 %) on the intestinal microbiome and metabolism in rainbow trout (Oncorhynchus mykiss) selected for growth and survival when fed a 100 % plant-based diet (suave) and a control line (temoin). Metabolic responses to the two factors (line and inulin) in liver, intestine, muscle and adipose were tissue-specific, with line and interaction between the two factors influencing overall expression in liver. In the intestine, inulin and line and in muscle, line influenced the expression of metabolic genes. Microbiota between the mucus and digestive contents was significantly different, with genera from Proteobacteria being more abundant in the mucus, whereas genera from the Firmicutes and Planctomycetes being more abundant in contents. Effect of inulin and interaction between factors on the microbiome was evident in contents. The significant taxa of control and inulin-fed groups differed greatly with Streptococcus and Weissella being significantly abundant in the inulin-fed group. There was a general trend showing higher levels of all SCFA in temoin group with propionic acid levels being significantly higher. An operational taxonomic unit (OTU) belonging to the Ruminococcaceae was significantly abundant in suave. The tissue-specific correlations between OTU and gene expression may indicate the link between microbiome and metabolism. Together, these results suggest that line and inulin impact the gene expression in a tissue-specific manner, possibly driven by specific OTUs enriched in inulin-fed groups and suave. [ABSTRACT FROM AUTHOR]

Published
2023
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16. Realised genetic gains on growth, survival, feed conversion ratio and quality traits after ten generations of multi-trait selection in rainbow trout Oncorhynchus mykiss, fed a standard diet or a “future” fish-free and soy-free diet

Author

Vandeputte, Marc, primary, Corraze, Geneviève, additional, Doerflinger, Jérôme, additional, Enez, Florian, additional, Clota, Frédéric, additional, Terrier, Frédéric, additional, Horat, Mathilde, additional, Larroquet, Laurence, additional, Petit, Vincent, additional, Haffray, Pierrick, additional, Skiba-Cassy, Sandrine, additional, and Dupont-Nivet, Mathilde, additional

Published
2022
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17. Molecular programming of the hepatic lipid metabolism via a parental high carbohydrate and low protein diet in rainbow trout

Author

Callet, Thérèse, primary, Li, Hongyan, additional, Heraud, Cécile, additional, Larroquet, Laurence, additional, Lanuque, Anthony, additional, Sandres, Franck, additional, Terrier, Frédéric, additional, Surget, Anne, additional, Corraze, Geneviève, additional, Panserat, Stéphane, additional, and Marandel, Lucie, additional

Published
2022
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18. Realised genetic gains on growth, survival, feed conversion ratio and quality traits after ten generations of multi-trait selection in rainbow trout Oncorhynchus mykiss, fed a standard diet or a “future” fish-free and soy-free diet

Author

Vandeputte, Marc, Corraze, Geneviève, Doerflinger, Jérôme, Enez, Florian, Clota, Frederic, Terrier, Frédéric, Horat, Mathilde, Larroquet, Laurence, Petit, Vincent, Haffray, Pierrick, Skiba-cassy, Sandrine, Dupont-nivet, Mathilde, Vandeputte, Marc, Corraze, Geneviève, Doerflinger, Jérôme, Enez, Florian, Clota, Frederic, Terrier, Frédéric, Horat, Mathilde, Larroquet, Laurence, Petit, Vincent, Haffray, Pierrick, Skiba-cassy, Sandrine, and Dupont-nivet, Mathilde

Abstract

There is limited scientific evidence on the real impact of selective breeding in aquaculture on the medium term, while the composition of aquafeeds is rapidly evolving towards plant-based raw materials. We compared a rainbow trout line selected in freshwater for fillet production (improved growth, carcass yield and fillet fat) for ten generations (G10) with an unselected control line from the same base population (G0). We crossed G10 and G0 neomales to the same G10 females, thus creating a Selected and a Control group expected to diverge by half the true difference between G0 and G10. Those were grown to 1.6 kg, and two feeds were compared across the two lines from 264 to 374 days post-hatching. One was a commercial standard, the second was a “future” feed devoid of fishmeal, fish oil and soy-based products, with microalgae as a source of docosahexaenoic acid (DHA). After doubling the difference between the Selected and the Control to estimate the true performance of G0, we saw that G10 was improved relative to G0 for body weight (+61%), feed conversion ratio (−17 to −20%), fillet fat (+28–53%) and carcass yield (+4.2%), but not for fillet yield. Survival was not affected by selection. Both feeds had a similar performance in terms of growth, but the future feed showed a higher FCR, probably due to a feed intake measurement issue. Fish had a good EPA+DHA content (>1.2 g/100 g wet weight) with both feeds, partly linked to endogenous synthesis of these fatty acids. There was little if any genotype by feed interaction. This study shows that selective breeding can produce fast growing, feed efficient and thus provide opportunity for more sustainable fish culture. We showed that highly nutritious fish can be produced with good growth performance without using any fish meal, fish oil or soy-based product.

Published
2022
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19. A parental low protein diet did not affect growth but modulate lipid metabolism in rainbow trout

Author

Callet, Thérèse, Li, H, Heraud, Cécile, Larroquet, Laurence, Lanuque, Anthony, Sandres, Franck, Terrier, Frédéric, Surget, Anne, Panserat, Stéphane, Marandel, Lucie, Nutrition, Métabolisme, Aquaculture (NuMéA), Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institute of Hydrobiology [Wuhan], Chinese Academy of Sciences [Beijing] (CAS), University of Chinese Academy of Sciences [Beijing] (UCAS), and European Aquaculture Society

Subjects
[SDV]Life Sciences [q-bio], [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

21. Réponse à 10 générations de sélection multicaractères chez la truite chez Aqualande

Author

Vandeputte, Marc, Corraze, Geneviève, Enez, Florian, Haffray, Pierrick, Doerflinger, Jérôme, Horat, Mathilde, Petit, Vincent, Terrier, Frédéric, Clota, Frédéric, Larroquet, Laurence, Espirat, Thierry, Skiba-Cassy, Sandrine, Dupont-Nivet, Mathilde, MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), Génétique Animale et Biologie Intégrative (GABI), Université Paris-Saclay-AgroParisTech-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Nutrition, Métabolisme, Aquaculture (NuMéA), Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Aqualande, and 3AS Station Expérimentale d'Aquaculture Ifremer, Chemin de Maguelone, 34250, Palavas-les-Flots, France

Subjects
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies, [SDV.SA.STP]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of fishery, [SDV.SA.ZOO]Life Sciences [q-bio]/Agricultural sciences/Zootechny, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

22. Effect of selenium sources in plant-based diets on antioxidant status and oxidative stress-related parameters in rainbow trout juveniles under chronic stress exposure

Author

Fontagné-Dicharry, Stéphanie, primary, Véron, Vincent, additional, Larroquet, Laurence, additional, Godin, Simon, additional, Wischhusen, Pauline, additional, Aguirre, Pierre, additional, Terrier, Frédéric, additional, Richard, Nadège, additional, Bueno, Maïté, additional, Bouyssière, Brice, additional, Antony Jesu Prabhu, Philip, additional, Tacon, Philippe, additional, and Kaushik, Sadasivam J., additional

Published
2020
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23. Suplementing aquafeeds with new alternative ingredients: a promising solution to improve plant-based diet efficiency in rainbow trout

Author

Skiba-Cassy, Sandrine, BUREL, Christine, Kerneis, Thierry, Labbé, Laurent, Terrier, Frédéric, Corraze, Geneviève, Richard, Nadège, Marchand, Y., Dupont-Nivet, Mathilde, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Phileo Lesaffre Animal Care, Coopérative Le Gouessant [Lamballe], Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, We thank FUI (Fonds Unique Interministériel) 2014 for financial support of the NINAqua project, European Aquaculture Society (EAS). BEL., and Le Gouessant

Subjects
oncorhynchus mykiss, alimentation végétale, alimentation animale, animal nutrition, growth, [SDV.BA]Life Sciences [q-bio]/Animal biology, animal feeding, nutrition animale, supplémentation alimentaire, rainbow trout, croissance, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

Session Nutrition: Additives and Ingredients; Suplementing aquafeeds with new alternative ingredients: a promising solution to improve plant-based diet efficiency in rainbow trout. Aquaculture Europe 2019

Published
2019

24. QTLs linked to survival and growth of rainbow trout fed a 100% plant-based diet in rainbow trout since the first meal

Author

Dechamp, Nicolas, D'Ambrosio, Jonathan, Terrier, Frédéric, Cachelou, Frédéric, Bestin, Anastasia, Quillet, Edwige, Skiba-Cassy, Sandrine, Médale, Françoise, Phocas, Florence, Dupont-Nivet, Mathilde, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Pisciculture Labedan, Viviers de Sarrance, Syndicat des Sélectionneurs Avicoles et Aquacoles Français, ANR Bioadapt: project 13-ADAP-001-01-Agreenfish, Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), and European Aquaculture Society (EAS). BEL.

Subjects
oncorhynchus mykiss, survie, alimentation végétale, animal nutrition, growth, [SDV.BA]Life Sciences [q-bio]/Animal biology, nutrition animale, selenium, rainbow trout, survival, croissance, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, feeding
Abstract

eposter; QTLs linked to survival and growth of rainbow trout fed a 100% plant-based diet in rainbow trout since the first meal. Aquaculture Europe 2019

Published
2019

25. Incorporation d'une fraction de levure riche en protéines dans une formule tout végétal chez la truite arc-en-ciel : impact sur les performances zootechniques et sur la santé intestinale

Author

Richard, Nadège, Fernandez-Boo, Sergio, Machado, Marina, Beutin, Catherine, Terrier, Frédéric, Costas, Benjamin, Skiba-Cassy, Sandrine, Phileo Lesaffre Animal Care, Centro Interdisciplinar de Investigaçao Marinha e Ambiental (CIIMA), Lesaffre International, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), and Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA.

Subjects
fish, salmonidae, trout, oncorhynchus mykiss, [SDV.BA]Life Sciences [q-bio]/Animal biology, yeast, rainbow trout, animal performance, poisson, rainbow, alimentation végétale, salmonids, performance zootechnique, levure, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, truite arc en ciel
Abstract

Session : Nutrition et Qualité; Incorporation d'une fraction de levure riche en protéines dans une formule tout végétal chez la truite arc-en-ciel : impact sur les performances zootechniques et sur la santé intestinale. 6. Journées de la Recherche Filière Piscicole

Published
2019

26. Nouveaux ingrédients et amélioration génétique : des solutions prometteuses pour améliorer les performances de croissance de la truite arc-en-ciel sur aliment végétal

Author

Skiba-Cassy, Sandrine, BUREL, Christine, Kerneis, Thierry, Labbé, Laurent, Terrier, Frédéric, Corraze, Geneviève, Richard, Nadège, Guimas, Laurence, Marchand, Y., Dupont-Nivet, Mathilde, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Phileo Lesaffre Animal Care, Copalis Industrie, Coopérative Le Gouessant [Lamballe], Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA., and Le Gouessant

Subjects
fish, salmonidae, amélioration génétique, trout, oncorhynchus mykiss, alimentation animale, [SDV.BA]Life Sciences [q-bio]/Animal biology, performance de croissance, rainbow trout, poisson, aquaculture, rainbow, animal nutrition, salmonids, novelty foods, animal feeding, nutrition animale, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, truite arc en ciel, aliment nouveau
Abstract

Session : Génétique et Sélection; Nouveaux ingrédients et amélioration génétique : des solutions prometteuses pour améliorer les performances de croissance de la truite arc-en-ciel sur aliment végétal. 6. Journées de la Recherche Filière Piscicole

Published
2019

27. Alimentation végétale supplémentée en DHA d'origine micro-algale au cours du cycle de reproduction de la truite arc-en-ciel : conséquences métaboliques chez les descendants

Author

Frohn, Laura, Panserat, Stephane, Cardona, Emilie, Larroquet, Laurence, Corraze, Geneviève, Labbé, Laurent, Terrier, Frédéric, Bobe, Julien, Skiba, Sandrine, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), FEAMP NutriEgg, Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA.

Subjects
fish, salmonidae, micro-algue, trout, oncorhynchus mykiss, alimentation animale, [SDV.BA]Life Sciences [q-bio]/Animal biology, acide docosahexaénoique, cycle de reproduction, rainbow trout, Docosahexaenoic acid, poisson, rainbow, alimentation végétale, salmonids, animal nutrition, animal feeding, nutrition animale, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, truite arc en ciel
Abstract

Session : Nutrition et Qualité; Alimentation végétale supplémentée en DHA d'origine micro-algale au cours du cycle de reproduction de la truite arc-en-ciel : conséquences métaboliques chez les descendants. 6. Journées de la Recherche Filière Piscicole

Published
2019

28. Impacts des traitements technologiques sur la valeur nutritionnelle de la graine de féverole pour la truite arc-en-ciel

Author

BUREL, Christine, Chesneau, Guillaume, Guillevic, Mathieu, Germain, Antoine, Terrier, Frédéric, Sandres, Franck, Lanuque, Anthony, Surget, Anne, Larroquet, Laurence, Pegourie, Gaia, Juin, Herve, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Valorex SAS, Elevage Alternatif et Santé des Monogastriques (UE EASM), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, trout, rainbow, féverole, valeur nutritionnelle des aliments, graine, traitement industriel, truite arc en ciel
Abstract

Impacts des traitements technologiques sur la valeur nutritionnelle de la graine de féverole pour la truite arc-en-ciel. 2. Rencontres Francophones sur les Légumineuses (RFL2)

Published
2018

29. NMR-based metabolomics workflow for characterization and effect assessment of alternative plant-based diets onplasma and microbiote in rainbow trout

Author

Deborde, Catherine, Madji Hounoum, Blandine, Jacob, Daniel, Maucourt, Mickael, Roques, Simon, Terrier, Frédéric, Gatesoupe, Francois Joël, Corraze, Geneviève, Médale, Françoise, Skiba-Cassy, Sandrine, Moing, Annick, Fauconneau, Benoit, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Phileo Lesaffre Animal Care, Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, ProdInra, Archive Ouverte, and Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)

Subjects
fish, approche métabolomique, trout, oncorhynchus mykiss, [SDV.BA] Life Sciences [q-bio]/Animal biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, rmn métabolique, rainbow trout, fish feeding, poisson, aquaculture, rainbow, animal nutrition, nutrition animale, micromass, alimentation des poisson, métabolisme, plasma, métabolomique, truite arc en ciel
Abstract

UMR BFP - Equipe Métabolisme; NMR-based metabolomics workflow for characterization and effect assessment of alternative plant-based diets onplasma and microbiote in rainbow trout. EUROMAR - European Magnetic Resonance Meeting

Published
2018

30. Effects of different dietary selenium sources on antioxidant status and oxidative stress-related parameters in rainbow trout juveniles fed plant ingredients

Author

Fontagne-Dicharry, Stephanie, Véron, Vincent, Godin, Simon, Larroquet, Laurence, Aguirre, Pierre, Terrier, Frédéric, Wischhusen, Pauline, Bueno, Maïté, Bouyssière, Brice, Richard, Nadège, Tacon, Philippe, Antony Jesu Prabhu, Philip, Kaushik, Sadasivam J., Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Centre National de la Recherche Scientifique (CNRS), Phileo Lesaffre Animal Care, and Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
endocrine system, oncorhynchus mykiss, animal structures, urogenital system, alimentation animale, animal diseases, [SDV.BA]Life Sciences [q-bio]/Animal biology, education, stress oxydatif, rainbow trout, protéine végétale, défense anti oxydante, plant protein, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, animal nutrition, oxidative stress, animal feeding, nutrition animale, selenium, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

Résumé; Effects of different dietary selenium sources on antioxidant status and oxidative stress-related parameters in rainbow trout juveniles fed plant ingredients. 18. ISFNF International Symposium on Fish Nutrition and Feeding "40 years of research in fish nutrition"

Published
2018

31. NMR-based metabolomics workflow for quality and effect assessment of alternative plant-based diets in rainbow trout

Author

DEBORDE, Catherine, MADJI HOUNOUM, Blandine, JACOB, Daniel, MAUCOURT, Mickael, ROQUES, Simon, TERRIER, Frédéric, CORRAZE, Geneviève, MÉDALE, Françoise, SKIBA-CASSY, Sandrine, MOING, Annick, FAUCONNEAU, Benoit, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Platetorme Métabolome Bordeaux, Institut National de la Recherche Agronomique (INRA), Nutrition, Métabolisme, Aquaculture (NuMéA), and Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA)

Subjects
[CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract

International audience

Published
2018

32. New sustainable protein sources in rainbow trout plant-based diets: consequences on growth performances, fatty acid content and immune-status

Author

Skiba-Cassy, Sandrine, BUREL, Christine, Terrier, Frédéric, Fauconneau, Benoit, Seiliez, Iban, Costas, Benjamin, Richard, Nadege, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Interdisciplinary Centre of Marine and Environmental research (CIIMAR), Phileo Lesaffre Animal Care, and European Aquaculture Society (EAS).

Subjects
Animal biology, oncorhynchus mykiss, statut immunitaire, [SDV.BA]Life Sciences [q-bio]/Animal biology, aquaculture durable, aliment à base de plante, yeast, performance de croissance, rainbow trout, teneur en acide gras, protéine, Biologie animale, protein, levure
Abstract

Résumé; The aquaculture sector has been subjected to a huge and sustained development during the last decades. The production of aquafeeds based on fishmeal and fish oils has been a key element in this development. However, the stability of the fishmeal and fish oil production is compromising the sustainable development of aquaculture. To face the decreasing availability of fishmeal and fish oil, the development of the aquaculture sector implied a search for new ingredient sources. As such, plant ingredients are nowadays being commonly incorporated in fish feed, allowing to spare consequent amount of fish meal and fish oil. However, the total replacement of fishmeal and fish oil has not been reached due to negative impacts on feed intake, metabolic and health status, flesh quality and growth performance slowdown. Single cell protein sources such as yeast protein products could be an alternative solution to complement and mitigate detrimental effects of plant proteins. This was tested in a trial in which rainbow trout juveniles were fed with a plant-based diet (devoid of fishmeal and fish oil), containing 0, 5, 10 or 15% of a yeast protein product (from Phileo Lesaffre Animal Care) for 12 weeks. In addition to yeast, microalgae containing high level of dososahexaenoic acid (DHA, 22:6 n-3) were added to each diet to cover n-3 long chain highly unsaturated fatty acids requirement of the fish. Mean bodyweight of fish was determined every 3 weeks and at the end of the feeding trial, whole body composition and fatty profiles were determined. Results indicated that yeast protein supplementation may upgrade growth performance of trout fed plant-based diets totally devoid of fishmeal and fish oil by improving feed efficiency and nitrogen retention. Surprisingly, it seems that yeast protein may also improve flesh quality by increasing DHA retention. Studies of cellular and molecular mechanisms are in progress to clarify the role of yeast protein on long chain highly unsaturated fatty acid biosynthesis. Immune status and gut health of the fish were also assessed through histological analyses of distal intestine and mRNA expression level measurements of immune-related genes in distal intestine. Those analyses were complemented by measurement of some plasma immune-related parameters. Results of this study revealed that inclusion of yeast proteins in a plant-based diet enables to improve rainbow trout gut health and global immune status. In conclusion, the present study highlights the potential benefits of adding yeast protein products in rainbow trout diet devoid of fishmeal and fish oil.

Published
2018

33. Agreenfish : adaptations aux transitions alimentaires en aquaculture: les caractériser et les favoriser

Author

Dupont-Nivet, Mathilde, Callet, Thérèse, Geurden, Inge, BUREL, Christine, Prunet, Patrick, Colson, Violaine, Lignot, Jehan-Hervé, BOREY, Marion, Lauga, Béatrice, Danion, Morgane, Geffroy, Benjamin, Terrier, Frédéric, Kerneis, Thierry, Le Calvez, Jean-Michel, Guyvarc'h, François, Goardon, Lionel, Labbé, Laurent, Quillet, Edwige, Médale, Françoise, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Université de Montpellier (UM), Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Centre National de la Recherche Scientifique (CNRS), Nielsen Company, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), ANR Agreenfish, Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Archive Ouverte

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2017

34. Feeding value of insect meals in trout and poultry: digestibility and effect on quality of products

Author

BUREL, Christine, Lessire, Michel, Juin, Herve, Rousseau, Philippe, Hallouis, Jean-Marc, Aguirre, Pierre, Terrier, Frédéric, Surget, Anne, Pegourie, Gaia, Meteau, Karine, Roy, Roselyne, Médale, Françoise, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Recherches Avicoles (SRA), Institut National de la Recherche Agronomique (INRA), Elevage Alternatif et Santé des Monogastriques (UE EASM), ANR DESIRABLE, and Unité de Recherches Avicoles (URA)

Subjects
Animal biology, fish, poultry, animal feed, insect meal, flour, farine, alimentation animale, digestibilité, qualité, valeur nutritionnelle des aliments, chicken, [SDV.BA]Life Sciences [q-bio]/Animal biology, fungi, poulet, insecta, poisson, digestibility, quality, Alimentation et Nutrition, Biologie animale, Food and Nutrition, animal feeding, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

Feeding value of insect meals in trout and poultry: digestibility and effect on quality of products. Insectinov 2 - Production d'insectes : Alimentation - Applications - Nouvelles filières industrielles

Published
2017

35. Valeur nutritionnelle de farine de tenebrion (Tenebrio molitor) et de farine d'hermetia (Hermetia illucens) dégraissées pour la truite arc-en-ciel (Oncorhynchus mykiss)

Author

BUREL, Christine, Aguirre, Pierre, Surget, Anne, Pegourie, Gaia, Terrier, Frédéric, Sandres, Franck, Lanuque, Anthony, Hubert, Antoine, Mezdour, Samir, Médale, Françoise, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Auteur indépendant, Ingénierie, Procédés, Aliments (GENIAL), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, ANR-ALID Systèmes Alimentaires Durables : projet DESIRABLE, and Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA.

Subjects
fish, oncorhynchus mykiss, hermetia illucens, valeur nutritionnelle des aliments, digestibilité, alimentation animale, [SDV.BA]Life Sciences [q-bio]/Animal biology, tenebrio, tenebrion, rainbow trout, poisson, digestibility, animal nutrition, animal feeding, nutrition animale, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

Résumé; Valeur nutritionnelle de farine de tenebrion (Tenebrio molitor) et de farine d'hermetia (Hermetia illucens) dégraissées pour la truite arc-en-ciel (Oncorhynchus mykiss). 5. Journées de la Recherche Filière Piscicole

Published
2016

36. Lipogenesis is mainly regulated by protein through a TORC1 dependent manner in rainbow trout liver and primary cell culture of hepatocytes

Author

Dai, Wei Wei, Panserat, Stéphane, Kaushik, Sadasivam, Terrier, Frédéric, Plagnes Juan, Elisabeth, Seiliez, Iban, Skiba-Cassy, Sandrine, Nutrition, Métabolisme, Aquaculture (NUMEA), Institut National de la Recherche Agronomique (INRA), and European Aquaculture Society (EAS). BEL.

Subjects
[SDV.BA]Life Sciences [q-bio]/Animal biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

38. Evaluation of fish oil replacement by coconut oil on feed intake, growth and fatty acid utilisation in rainbow trout

Author

Figueiredo-Silva, A.C., Kaushik, Sadasivam, Terrier, Frédéric, Geurden, Inge, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], PRISE ALIMENTAIRE, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2010

39. Rainbow trout regulates lean body growth but does not adjust feed intake of diets non-limiting in protein with varying lipid levels

Author

Geurden, Inge, Schrama, J.W., Terrier, Frédéric, Médale, Françoise, Kaushik, S.J., Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Aquaculture and Fisheries Group, Wageningen Institute of Animal Science, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], FEED INTAKE, [SDV]Life Sciences [q-bio], PRISE ALIMENTAIRE, [INFO]Computer Science [cs], DP/DE, DIETARY LIPIDS, [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, SALMONIDE
Abstract

International audience

Published
2010

40. Responses of rainbow trout clonal lines to plant-based feed evidence genotype x diet interactions

Author

Médale, Françoise, Dupont-Nivet, Mathilde, Rimoldi, S., Yilmaz, Hatice Asuman, Terrier, Frédéric, Corraze, Geneviève, Quillet, Edwige, Geurden, Inge, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, and ProdInra, Archive Ouverte

Subjects
[SDV] Life Sciences [q-bio], BODY COMPOSITION, NUTRIENT UTILIZATION, GENETIQUE ANIMALE, [SDV]Life Sciences [q-bio], FISH MEAL AND FISH OIL SUBSTITUTION
Abstract

absent

Published
2010

42. Ingestion, croissance et efficacité alimentaire chez la truite arc-en-ciel nourrie avec un aliment sans farine de poisson

Author

Geurden, Inge, Kaushik, Sadasivam, Terrier, Frédéric, Médale, Françoise, ProdInra, Migration, Nutrition, Aquaculture et Génomique (NUAGE), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2007

44. Hepatic fatty acid biosynthesis is more responsive to protein than carbohydrate in rainbow trout during acute stimulations.

Author

Weiwei Dai, Panserat, Stéphane, Kaushik, Sadasivam, Terrier, Frédéric, Plagnes-Juan, Elisabeth, Seiliez, Iban, and Skiba-Cassy, Sandrine

Abstract

The link between dietary carbohydrate/protein and de novo lipogenesis (DNL) remains debatable in carnivorous fish. We aimed to evaluate and compare the response of hepatic lipogenic gene expression to dietary carbohydrate intake/glucose and dietary protein intake/amino acids (AAs) during acute stimulations using both in vivo and in vitro approaches. For the in vivo trial, three different diets and a controlled-feeding method were employed to supply fixed amount of dietary protein or carbohydrate in a single meal; for the in vitro trial, primary hepatocytes were stimulated with a low or high level of glucose (3 mM or 20 mM) and a low or high level of AAs (one-fold or four-fold concentrated AAs). In vitro data showed that a high level of AAs upregulated the expression of enzymes involved in DNL [fatty acid synthase (FAS) and ATP citrate lyase (ACLY)], lipid bioconversion [elongation of very long chain fatty acids like-5 (Elovl5), Elovl2, Δ6 fatty acyl desaturase (D6D) and stearoyl-CoA desaturase-1 (SCD1)], NADPH production [glucose-6-phosphate dehydrogenase (G6PDH) and malic enzyme (ME)], and transcriptional factor sterol regulatory element binding protein 1-like, while a high level of glucose only elevated the expression of ME. Data in trout liver also showed that high dietary protein intake induced higher lipogenic gene expression (FAS, ACLY, and Elovl2) regardless of dietary carbohydrate intake, while high carbohydrate intake markedly suppressed the expression of acetyl-CoA carboxylase (ACC) and Elovl5. Overall, we conclude that, unlike rodents or humans, hepatic fatty acid biosynthetic gene expression in rainbow trout is more responsive to dietary protein intake/AAs than dietary carbohydrate intake/glucose during acute stimulations. This discrepancy probably represents one important physiological and metabolic difference between carnivores and omnivores. [ABSTRACT FROM AUTHOR]

Published
2016
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46. Acute rapamycin treatment improved glucose tolerance through inhibition of hepatic gluconeogenesis in rainbow trout (Oncorhynchus mykiss).

Author

Weiwei Dai, Panserat, Stéphane, Terrier, Frédéric, Seiliez, Iban, and Skiba-Cassy, Sandrine

Subjects
RAPAMYCIN, GLUCOSE tolerance tests, REGULATION of gluconeogenesis, STEELHEAD trout, INTRAPERITONEAL injections
Abstract

Our aim was to investigate the potential role of TOR (target of rapamycin) signaling pathway in the regulation of hepatic glucose metabolism in rainbow trout. Fasted fish were first treated with a single intraperitoneal injection of rapamycin or vehicle and then submitted to a second intraperitoneal administration of glucose 4 h later. Our results revealed that intraperitoneal administration of glucose induced hyperglycemia for both vehicle and rapamycin treatments, which peaked at 2 h. Plasma glucose level in vehicle-treated fish was significantly higher than in rapamycin-treated fish at 8 and 17 h, whereas it remained at the basal level in rapamycin-treated fish. Glucose administration significantly enhanced the phosphorylation of Akt and ribosomal protein S6 kinase (S6K1) in vehicle-treated fish, while rapamycin completely abolished the activation of S6K1 in rapamycin-treated fish, without inhibiting the phosphorylation of Akt on Thr-308 or Ser-473. Despite the lack of significant variation in phosphoenolpyruvate carboxykinase mRNA abundance, mRNA abundance for glucokinase (GK), glucose 6-phosphatase (G6Pase) I and II, and fructose 1,6-bisphosphatase (FBPase) was reduced by rapamycin 17 h after glucose administration. The inhibition effect of rapamycin on GK and FBPase was further substantiated at the activity level. The suppression of GK gene expression and activity by rapamycin provided the first in vivo evidence in fish that glucose regulates hepatic GK gene expression and activity through a TORC1-dependent manner. Unlike in mammals, we observed that acute rapamycin treatment improved glucose tolerance through the inhibition of hepatic gluconeogenesis in rainbow trout. [ABSTRACT FROM AUTHOR]

Published
2014
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47. Post-prandial regulation of hepatic glucokinase and lipogenesis requires the activation of TORC1 signalling in rainbow trout (Oncorhynchus mykiss).

Author

Dai, Weiwei, Panserat, Stéphane, Mennigen, Jan A., Terrier, Frédéric, Dias, Karine, Seiliez, Iban, and Skiba-Cassy, Sandrine

Subjects
RAINBOW trout, GLUCOKINASE, LIPOGENESIS in fishes, CELLULAR signal transduction, GENE expression in fishes, PHOSPHORYLATION, PHYSIOLOGY, FISHES
Abstract

To assess the potential involvement of TORC1 (target of rapamycin complex 1) signalling in the regulation of post-prandial hepatic lipid and glucose metabolism-related gene expression in trout, we employed intraperitoneal administration of rapamycin to achieve an acute inhibition of the TOR pathway. Our results reveal that rapamycin inhibits the phosphorylation of TORC1 and its downstream effectors (S6K1, S6 and 4E-BP1), without affecting Akt and the Akt substrates Forkhead-box Class O1 (FoxO1) and glycogen synthase kinase 3a/ß (GSK 3a/ß). These results indicate that acute administration of rapamycin in trout leads to the inhibition of TORC1 activation. No effect is observed on the expression of genes involved in gluconeogenesis, glycolysis and fatty acid oxidation, but hepatic TORC1 inhibition results in decreased sterol regulatory element binding protein 1c (SREBP1c) gene expression and suppressed fatty acid synthase (FAS) and glucokinase (GK) at gene expression and activity levels, indicating that FAS and GK activity is controlled at a transcriptional level in a TORC1-dependent manner. This study demonstrates for the first time in fish that post-prandial regulation of hepatic lipogenesis and glucokinase in rainbow trout requires the activation of TORC1 signalling. [ABSTRACT FROM AUTHOR]

Published
2013
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48. Link between lipid metabolism and voluntary food intake in rainbow trout fed coconut oil rich in medium-chain TAG.

Author

Figueiredo-Silva, A. Cláudia, Kaushik, Sadasivam, Terrier, Frédéric, Schrama, Johan W., Médale, Françoise, and Geurden, Inge

Subjects
ANALYSIS of variance, ANIMAL experimentation, BLOOD sugar, BODY composition, CHOLECYSTOKININ, FATTY acids, FISHES, INGESTION, POLYMERASE chain reaction, PROBABILITY theory, RESEARCH funding, SATISFACTION, TRIGLYCERIDES, VEGETABLE oils, DATA analysis software, GENE expression profiling, NUTRITIONAL status
Abstract

We examined the long-term effect of feeding coconut oil (CO; rich in lauric acid, C12) on voluntary food intake and nutrient utilisation in rainbow trout (Oncorhynchus mykiss), with particular attention to the metabolic use (storage or oxidation) of ingested medium-chain TAG. Trout were fed for 15 weeks one of the four isoproteic diets containing fish oil (FO) or CO as fat source (FS), incorporated at 5 % (low fat, LF) or 15 % (high fat, HF). Fat level or FS did not modify food intake (g/kg0·8 per d), despite higher intestinal cholecystokinin-T mRNA in trout fed the HF-FO diet. The HF diets relative to the LF ones induced higher growth and adiposity, whereas the replacements of FO by CO resulted in similar growth and adiposity. This, together with the substantial retention of C12 (57 % of intake), suggests the relatively low oxidation of ingested C12. The down-regulation of carnitine palmitoyl-transferase-1 (CPT-1) confirms the minor dependency of medium-chain fatty acids (MCFA) on CPT-1 to enter the mitochondria. However, MCFA did not up-regulate mitochondrial oxidation evaluated using hepatic hydroxyacyl-CoA dehydrogenase as a marker, in line with their high retention in body lipids. At a low lipid level, MCFA increased mRNA levels of fatty acid synthase, elongase and stearoyl-CoA desaturase in liver, showing the hepatic activation of fatty acid synthesis pathways by MCFA, reflected by increased 16 : 0, 18 : 0, 16 : 1, 18 : 1 body levels. The high capacity of trout to incorporate and transform C12, rather than to readily oxidise C12, contrasts with data in mammals and may explain the absence of a satiating effect of CO in rainbow trout. [ABSTRACT FROM AUTHOR]

Published
2012
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49. Discarded blue mussel (<italic>Mytilus edulis</italic>): a feed ingredient that maintains growth performance of juvenile gilthead seabream (<italic>Sparus aurata</italic>) while fishmeal and fish oil are removed.

Author

Jaeger, Christophe, Corraze, Geneviève, Gayet, Vincent, Larroquet, Laurence, Surget, Anne, Terrier, Frédéric, and Aubin, Joël

Subjects
*ESSENTIAL fatty acids, *SPARUS aurata, *ESSENTIAL amino acids, *DOCOSAHEXAENOIC acid, *FISH oils, *FISH feeds, *EICOSAPENTAENOIC acid
Abstract

The total substitution of fishmeal (FM) and fish oil (FO) with sustainable feed ingredients, while maintaining acceptable growth performances and fish composition, is a challenge. Mussels have a high protein content, with essential amino acid and fatty acid (FA) profiles similar to those of FM. This study aimed to assess the potential to rear gilthead seabream (Sparus aurata) on a plant-based feed (without FM and FO) to which mussels were added. Subsequent effects on growth performance, protein content, and FA concentrations were studied. Juvenile gilthead seabream were reared for 46 days in water temperature of 21°C, in a recirculated aquaculture system composed of three fish tanks, each corresponding to one treatment: in the FMFO treatment, 342 fish were fed a commercial feed that contained FM and FO; in the plant-based feed (PBF) treatment, 345 fish were fed an experimental feed that contained only plant-based ingredients; in the mussel feed (MF) treatment, 342 fish were fed the feed from PBF supplemented with fresh mussel flesh from which the shells had been removed. Fish weighed a mean of 6.7 ± 1.3 g at stocking in each treatment. At harvest, the mean weight of fish from FMFO and MF were significantly different (23.1 ± 4.3 and 23.1 ± 5.2 g, respectively) than that from PBF (15.5 ± 3.6 g) (p < .05). Compared to the feed from FMFO, the feed from PBF decreased the percentages of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and saturated FA (14:0, 16:0) in fish, but increased those of 18:1, 18:2 n-6 and 18:3 n-3 FA. Compared to the feed from PBF, the feed from MF increased the EPA+DHA concentration in fish by 21%. Thus, adding fresh mussels to a plant-based feed yielded similar growth performances and survival rate as a commercial feed. It also improved the FA composition of fish compared to that of fish fed a plant-based feed. [ABSTRACT FROM AUTHOR]

Published
2024
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50. Hepatic fatty acid biosynthesis is more responsive to protein than carbohydrate in rainbow trout during acute stimulations.

Author

Dai W, Panserat S, Kaushik S, Terrier F, Plagnes-Juan E, Seiliez I, and Skiba-Cassy S

Subjects
Amino Acids metabolism, Animal Nutritional Physiological Phenomena, Animals, Cells, Cultured, Dietary Carbohydrates administration & dosage, Dietary Proteins administration & dosage, Fish Proteins genetics, Fish Proteins metabolism, Gene Expression Regulation, Enzymologic, Glucose metabolism, Hepatocytes drug effects, Hepatocytes enzymology, Insulin metabolism, Mechanistic Target of Rapamycin Complex 1, Multiprotein Complexes metabolism, Nutritional Status, Oncorhynchus mykiss genetics, Primary Cell Culture, Protein Kinase Inhibitors pharmacology, Signal Transduction, Sirolimus pharmacology, TOR Serine-Threonine Kinases antagonists & inhibitors, TOR Serine-Threonine Kinases metabolism, Time Factors, Dietary Carbohydrates metabolism, Dietary Proteins metabolism, Fatty Acids biosynthesis, Hepatocytes metabolism, Lipogenesis drug effects, Lipogenesis genetics, Oncorhynchus mykiss metabolism
Abstract

The link between dietary carbohydrate/protein and de novo lipogenesis (DNL) remains debatable in carnivorous fish. We aimed to evaluate and compare the response of hepatic lipogenic gene expression to dietary carbohydrate intake/glucose and dietary protein intake/amino acids (AAs) during acute stimulations using both in vivo and in vitro approaches. For the in vivo trial, three different diets and a controlled-feeding method were employed to supply fixed amount of dietary protein or carbohydrate in a single meal; for the in vitro trial, primary hepatocytes were stimulated with a low or high level of glucose (3 mM or 20 mM) and a low or high level of AAs (one-fold or four-fold concentrated AAs). In vitro data showed that a high level of AAs upregulated the expression of enzymes involved in DNL [fatty acid synthase (FAS) and ATP citrate lyase (ACLY)], lipid bioconversion [elongation of very long chain fatty acids like-5 (Elovl5), Elovl2, Δ6 fatty acyl desaturase (D6D) and stearoyl-CoA desaturase-1 (SCD1)], NADPH production [glucose-6-phosphate dehydrogenase (G6PDH) and malic enzyme (ME)], and transcriptional factor sterol regulatory element binding protein 1-like, while a high level of glucose only elevated the expression of ME. Data in trout liver also showed that high dietary protein intake induced higher lipogenic gene expression (FAS, ACLY, and Elovl2) regardless of dietary carbohydrate intake, while high carbohydrate intake markedly suppressed the expression of acetyl-CoA carboxylase (ACC) and Elovl5. Overall, we conclude that, unlike rodents or humans, hepatic fatty acid biosynthetic gene expression in rainbow trout is more responsive to dietary protein intake/AAs than dietary carbohydrate intake/glucose during acute stimulations. This discrepancy probably represents one important physiological and metabolic difference between carnivores and omnivores., (Copyright © 2016 the American Physiological Society.)

Published
2016
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