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Abstract The synthesis of copper nanowires (CuNWs) using modified polyols with hexadecylamine or octadecylamine as capping agents, known for their ease of operation, rapid reaction, and suitability for mass production, often encounters challenges such as the presence of copper particles, numerous copper nanorods, difficulty in purifying organic compounds, and air instability as by-products. In addressing these issues, this study introduces an innovative multiphase ultrasonic-assisted separation method. By utilizing ultrasound to accelerate the dissolution of hexadecylamine and facilitate the separation movement of CuNWs and by-products in hydrophilic and hydrophobic phases, the aim of achieving high purification of CuNWs is realized. Through the introduction of an ultra-thin hexadecylamine layer (~ 3.5 nm) on the surface of CuNWs and leveraging the active adsorption of silver seed crystals, combined with the control CuNWs reaction concentration, silver-coated CuNWs with a dense and uniform surface thickness of 12–15 nm are obtained. By enhancing purity, stability, and cost-effectiveness, this research represents a significant advancement in the field of one-dimensional nanowires and their devices.

Abstract Background Small cell lung cancer (SCLC) stands as one of the most lethal malignancies, characterized by a grim diagnosis and prognosis. The emergence of multi-drug resistance poses a significant hurdle to effective therapy. Although previous studies have implicated the long noncoding RNA LYPLAL1-DT in the tumorigenesis of SCLC, the precise role of the highly expressed LYPLAL1-DT in SCLC chemoresistance and the underlying mechanism remain inadequately understood. Methods cDDP-, VP-16- and PTX-resistant SCLC cells lines were established. The viabilities of SCLC cells were assessed by CCK-8 assay in vitro and xenograft tumor formation assay in vivo. Apoptosis was evaluated by FACS, Western blot and JC-1 fluorescence staining, while autophagy was explored via autophagic flux detection under confocal microscopy and autophagic vacuole investigation under transmission electron microscopy (TEM). The functional role and mechanism of LYPLAL1-DT were further investigated by gain- and loss-of-function assays in vitro. Furthermore, the therapeutic efficacy of the combination of venetoclax and HCQ with cDDP, VP-16 or PTX was evaluated by cell line, cell-derived xenograft (CDX) and patient-derived xenograft (PDX) mice model. Results Our findings revealed that LYPLAL1-DT is upregulated in chemoresistant SCLC cell lines. Gain- and loss-of-function assays demonstrated that LYPLAL1-DT impairs sensitivity to cDDP, VP-16, or PTX both in vitro and in vivo. Overexpression of LYPLAL1-DT significantly enhanced autophagy and inhibited apoptosis in SCLC cells. Further analyses, including RIP and RNA pull-down assays, revealed that LYPLAL1-DT promotes the expression of BCL2 by sponging miR-204-5p and is implicated in the assembly of the autophagy-specific complex (BECN1/PtdIns3K complex). Combining venetoclax and HCQ with cDDP, VP-16, or PTX effectively mitigated chemoresistance in SCLC cells and suppressed tumor growth in CDX and PDX models without inducing obvious toxic effects. Conclusions Our findings demonstrate that upregulation of LYPLAL1-DT sequesters apoptosis through the LYPLAL1-DT/miR-204-5p/BCL2 axis and promotes autophagy by facilitating the assembly of the BECN1/PtdIns3K complex, thereby mediating multi-drug resistance of SCLC. The triple combination of venetoclax, HCQ, in conjunction with cDDP, VP-16 or PTX overcomes refractory SCLC, shedding light on a potential therapeutic target for combating SCLC chemoresistance.

Adiabatic shearing represents a prevalent mode of deformation failure in metals when subjected to impact loading. In this work, dynamic shearing interruption tests considering various shear displacements (i.e., shear strains) were conducted on of the CoCrFeNi high-entropy alloy (HEA) produced by laser powder bed fusion (LPBF) to analyze the microstructural evolution during adiabatic shearing. From the results, it was observed that the columnar grains in the shear region only distorted to different degrees at the lower levels of shear strain. Then, it was also found that the increase in shear strain caused severe distortion and refinement of the columnar grains, ultimately leading to adiabatic shearing band (ASB) at a critical shear strain threshold. During inhomogeneous deformation at high strain rate, the ASB formation was predicated on enough plastic deformation work. The interconnection between microstructural softening mechanisms and macroscopic mechanical behavior during ASB evolution and fracture was also highlighted. The nano-sized equiaxed recrystallized grains within the ASB were formed by rotational dynamic recrystallization (RDRX) rather than migratory recrystallization. Moreover, a large number of nano-twins were formed in these nano-sized recrystallized grains to motivate the mechanism of slip within nano-twins, thus accommodating the continuous plastic deformation. These results are the main reasons for the outstanding resistance to shear deformation of CoCrFeNi HEA produced by LPBF.

Abstract Although quantum dot light‐emitting diodes (QLEDs) are extensively studied nowadays, their charge transport mechanism remains a subject of ongoing debate. Here, the hole transport in blue quantum dots (QDs) (CdZnSe/ZnSe/ZnS/CdZnS/ZnS based) is investigated by combining current‐voltage and transient electroluminescence measurements. The study demonstrates that the hole transport in QD thin films is characterized by a trap‐free space‐charge‐limited current with a zero‐field room temperature mobility of 4.4 × 10−11 m2 V−1 s−1. The zero‐field hole mobility is thermally activated with an activation energy of 0.30 eV. Applying the Extended Gaussian Disorder model provides a consistent description of the QD hole current as a function of voltage and temperature. The QD hole mobility is characterized by a hopping distance of 2.8 nm in a Gaussian broadened density of states with a width of 0.12 eV.

Abstract Icing of seawater droplets is capable of causing catastrophic damage to vessels, buildings, and human life, yet it also holds great potential for enhancing applications such as droplet-based freeze desalination and anti-icing of sea sprays. While large-scale sea ice growth has been investigated for decades, the icing features of small salty droplets remain poorly understood. Here, we demonstrate that salty droplet icing is governed by salt rejection-accompanied ice crystal growth, resulting in freezing dynamics different from pure water. Aided by the observation of brine films emerging on top of frozen salty droplets, we propose a universal definition of freezing duration to quantify the icing rate of droplets having varying salt concentrations. Furthermore, we show that the morphology of frozen salty droplets is governed by ice crystals that sprout from the bottom of the brine film. These crystals grow until they pierce the free interface, which we term ice sprouting. We reveal that ice sprouting is controlled by condensation at the brine film free interface, a mechanism validated through molecular dynamics simulations. Our findings shed light on the distinct physics that govern salty droplet icing, knowledge that is essential for the development of related technologies.

ABSTRACT: Fluoride is one of the essential trace elements for body. However, excessive fluoride poses a major threat to human and animal health. Fluorosis may cause pathological damage of the duodenum, but the underlying mechanism needs to be further studied. This study was to investigate the effects of long-term exposure to sodium fluoride (0, 500, 1,000, 2,000 mg/kg) on the duodenum of chickens. The results showed that after NaF exposure, intestinal epithelial cells were disarranged, necrotic or even exfoliated, goblet cells and mucus secretion were increased, and inflammatory response was induced in duodenal tissue. Oxidative stress, endoplasmic reticulum stress (ERs), and heat shock proteins (HSPs) are an adaptive response, however long-term, excessive changes are detrimental. Fluorosis activates ERs through IRE1, PERK and ATF6 pathways, increases the expression of HSP60, HSP70 and HSP90, and causes apoptosis and oxidative damage in duodenal tissue. In addition, fluorosis can activate the MAPK signaling pathway. This article can provide a reference for exploring the potential duodenal toxicity of sodium fluoride.

Eukaryotic elongation factors (eEFs) are protein factors that mediate the extension of peptide chain, among which eukaryotic elongation factor 1 alpha (eEF1A) is one of the most abundant protein synthesis factors. Previously we showed that the P3 protein of Soybean mosaic virus (SMV), one of the most destructive and successful viral pathogens of soybean, targets a component of the soybean translation elongation complex to facilitate its pathogenesis. Here, we conducted a systematic analyses of the soybean eEF (GmeEF) gene family in soybean and examinedits role in virus resistance. In this study, GmeEF family members were identified and characterized based on sequence analysis. The 42 members, which were unevenly distributed across the 15 chromosomes, were renamed according to their chromosomal locations. The GmeEF members were further divided into 12 subgroups based on conserved motif, gene structure, and phylogenetic analyses. Analysis of the promoter regions showed conspicuous presence of myelocytomatosis (MYC) and ethylene-responsive (ERE) cis-acting elements, which are typically involved in drought and phytohormone response, respectively, and thereby in plant stress response signaling. Transcriptome data showed that the expression of 15 GmeEF gene family members changed significantly in response to SMV infection. To further examine EF1A function in pathogen response, three different Arabidopsis mutants carrying T-DNA insertions in orthologous genes were analyzed for their response to Turnip crinkle virus (TCV) and Cucumber mosaic virus (CMV). Results showed that there was no difference in viral response between the mutants and the wild type plants. This study provides a systematic analysis of the GmeEF gene family through analysis of expression patterns and predicted protein features. Our results lay a foundation for understanding the role of eEF gene in soybean anti-viral response.

Neutrophils are heterogeneous and plastic, with the ability to polarize from antitumour to protumour phenotype and modulate tumour microenvironment components. While some advances have been made, the neutrophil-targeting therapy remains underexplored. Activation of formyl peptide receptors (FPRs) by formylated peptides is needed for local control of infection through the recruitment of activated neutrophils while the potential contribution of antitumour activity remains underexplored. Here, we demonstrate that neutrophils can be harnessed to suppress tumour growth through the action of the formyl peptide (FP) on the formyl peptide receptor (FPR). Mechanistically, FP efficiently recruits neutrophils to produce reactive oxygen species production (ROS), resulting in the direct killing of tumours. Antitumour functions disappeared when neutrophils were depleted by anti-Ly6G antibodies. Interestingly, extensive T-cell activation was observed in mouse tumours treated with FP, showing the potential to alter the immune suppressed tumour microenvironment (TME) and further sensitize mice to anti-PD1 therapy. Transcriptomic and flow cytometry analyses revealed the mechanisms of FP-sensitized anti-PD1 therapy, mainly including stimulated neutrophils and an altered immune-suppressed tumour microenvironment. Collectively, these data establish FP as an effective combination partner for sensitizing anti-PD1 therapy by stimulating tumour-infiltrated neutrophils.

IntroductionThe effect of intergroup contact on cooperation is well documented, but little is known about the cultural moderators of this relationship. Contributing to the literature, we examined whether cultural orientation moderates the effect of positive intergroup contact on cooperation and places emphasis on individualism.MethodsBy creating a gamecooperation situation by the trust game paradigm, 322 Taiwanese youth were involved in the study and completed the positive intergroup contact scale, individual-collectivism scale, and social distance scale.Results(1) positive intergroup contact effectively promotes cooperative behavior; (2) Taiwanese youth who have closer social distance with mainland youth demonstrate higher levels of cooperative behavior after group interactions than larger social distance; and (3) individualism has a significant moderating role in the relationship between positive inter-group contact and cooperation. The effect of positive inter-group contact on cooperation became stronger in the less individualistic group. The effect of social distance on cooperation became stronger in the less individualistic group.Cultural orientation represented by individualism is proved to be one moderato of the intergroup contact-cooperation relationship.

Abstract The transit signal priority leads to the delay of private vehicles in the priority and non-priority phases. To address this problem, a bi-level programming model is proposed based on the dynamic cycle and arrival rate of private vehicles under connected environment. The upper model is built by a delay triangle, with the maximum delay reduction of private vehicles between the decreased delay and increased delay in the experimental period. The lower model is constructed based on the Stackelberg model of game theory, and the objective is to obtain the dynamic cycle. A genetic algorithm (GA) is implemented to solve the proposed model. Based on SUMO, a case study of a self-driving bus in the city of Zhengzhou is conducted to demonstrate the effectiveness of the proposed model. The results from GA and SUMO are consistent, which verifies the effectiveness of the proposed model. The delay of the private vehicles with dynamic signal priority declines by 21.32% on average compared to that without priority. Compared with active signal priority, it declines by 22.63% on average. The proposed method is compared with the method proposed by other papers, and the delay per private vehicle is small. The effectiveness of the proposed method is further illustrated. The proposed methodology is helpful for improving the operation efficiency of intersections with minimum delay.

Abstract Objective To observe and evaluate the effectiveness and safety of Esketamine or Sufentanil combined with Dexmedetomidine for sedation and analgesia in lung tumor percutaneous radiofrequency ablation (PRFA) to provide a clinical basis for the optimization of sedation and analgesia in lung tumor PRFA protocols outside the operating room. Methods In this trial, 44 patients aged 37 to 84 undergoing lung tumor PRFA were enrolled and assigned to Group E (n = 22, Esketamine 0.2 mg/kg) or Group S (n = 22,Sufentanil 0.1 μg/kg ). Dexmedetomidine was infused intravenously as a sedative in both groups. The modified observer’s assessment of alertness and sedation scale (MOAAS), physical movement pain scale, intraoperative vital signs, anesthesia recovery time, radiologist and patient satisfaction rates, incidence of respiratory depression, and incidence of postoperative nausea and vomiting were recorded. Results Although there was no significant difference in the physical movement pain scale, blood oxygen saturation or incidence of perioperative adverse events between the two groups during ablation, the MOAAS, mean arterial pressure (MAP) and heart rate (HR) were higher in Group E than in Group S. The anesthesia recovery time was shorter in Group E than in Group S, and radiologist satisfaction was better in Group E than in Group S, but there was no significant difference between the two groups in terms of patient satisfaction. Conclusion Esketamine or Sufentanil combined with Dexmedetomidine is safe for lung tumor PRFA. However, in elderly patients with multiple underlying diseases, low-dose Esketamine combined with Dexmedetomidine has fewer hemodynamic effects on patients, milder respiratory depression, shorter recovery time, and better radiologist satisfaction because of its better controllability of sedation depth. Trial registration Chinese Clinical Trial Registry (Registration number#ChiCTR ChiCTR21000500 21); Date of Registration: 16/08/2021

Introduction: Pulmonary fibrosis (PF) is a fatal chronic lung disease that causes structural damage and decreased lung function and has a poor prognosis. Currently, there is no medicine that can truly cure PF. Vitamin E (VE) is a group of natural antioxidants with anticancer and antimutagenic properties. There have been a few reports about the attenuation of PF by VE in experimental animals, but the molecular mechanisms are not fully understood.Methods: Bleomycin-induced PF (BLM-PF) mouse model, and cultured mouse primary lung fibroblasts and MLE 12 cells were utilized. Pathological examination of lung sections, immunoblotting, immunofluorescent staining, and real-time PCR were conducted in this study.Results: We confirmed that VE significantly delayed the progression of BLM-PF and increased the survival rates of experimental mice with PF. VE suppressed the pathological activation and fibrotic differentiation of lung fibroblasts and epithelial-mesenchymal transition and alleviated the inflammatory response in BLM-induced fibrotic lungs and pulmonary epithelial cells in vitro. Importantly, VE reduced BLM-induced ferritin expression in fibrotic lungs, whereas VE did not exhibit iron chelation properties in fibroblasts or epithelial cells in vitro. Furthermore, VE protected against mitochondrial dysmorphology and normalized mitochondrial protein expression in BLM-PF lungs. Consistently, VE suppressed apoptosis in BLM-PF lungs and pulmonary epithelial cells in vitro.Discussion: Collectively, VE markedly inhibited BLM-induced PF through a complex mechanism, including improving iron metabolism and mitochondrial structure and function, mitigating inflammation, and decreasing the fibrotic functions of fibroblasts and epithelial cells. Therefore, VE presents a highly potential therapeutic against PF due to its multiple protective effects with few side effects.

Patients with double-mutated CEBPA (CEBPAdm) AML were stratified into favorable risk group, however, few studies have investigated the heterogeneity of different CEBPAdm types in detail. In this study, we analyzed 2211 newly diagnosed AML and identified CEBPAdm in 10.8% of the patients. Within the CEBPAdm cohort, 225 of 239 patients (94.14%) presented with bZIP region mutations (CEBPAdmbZIP) while 14 of 239 patients (5.86%) without bZIP region mutation (CEBPAdmnonbZIP). Analysis of the accompanied molecular mutations showed statistically different incidences of GATA2 mutations between the CEBPAdmbZIP group and the CEBPAdmnonbZIP group (30.29% vs 0%). In the analysis of outcomes, patients with CEBPAdmnonbZIP were associated with shorter overall survival (OS) censored at hematopoietic stem cell transplantation (HSCT) during CR1 compared to those with CEBPAdmbZIP (hazard ratio (HR) = 3.132, 95% confidence interval (CI) = 1.229–7.979, P = .017). Refractory or relapsed AML (R/RAML) patients with CEBPAdmnonbZIP were associated with shorter OS compared to those with CEBPAdmbZIP (HR = 2.881, 95% CI = 1.021–8.131, P = .046). Taken together, AML with CEBPAdmbZIP and CEBPAdmnonbZIP showed different outcomes and might be regarded as distinctive AML entities.

On the southeastern margin of the Ordos Basin, the mineral composition of marine-continent transitional facies deposits is complex. The shale, sandstone, coal, and related lithofacies frequently interact, and the lithology changes rapidly in the vertical direction. Due to the low resolution of conventional logging method and borehole enlargement which is a common while drilling, the commonly used methods for identification of lithology including high-quality shale which is prevailing in marine shale gas evaluation are less effective for the study area. First, deconvolution technology was used to improve the resolution of natural gamma rays, gamma rays without uranium and uranium logging curves. Then, a log cross-plot was used to identify the lithology including shale of marine-continent transitional facies, and the uranium-spontaneous potential curve overlap method was proposed to identify high-quality shale from marine-continent transitional facies. The results show that the deconvolution method can effectively improve the vertical resolution of natural gamma rays, gamma rays without uranium and uranium logging curves. The cross-plot of natural gamma-density logging data has a better effect on identifying the lithology of the marine-continent transitional facies, and the cross-plot of uranium logging data and gamma ray data without uranium can further identify three types of shale lithofacies (calcareous siliceous shale, siliceous clay shale and clay shale). In the marine-continent transitional facies, the newly proposed uranium-spontanous potential overlap method is better than the traditional ΔlogR method inidentifying high-quality shale. This research can provide theoretical support for reservoir evaluation of marine-continent transitional shale gas and improve the accuracy of high-quality shale identification.

The post-heat mechanical property is one of the important indices for the fire-resistance evaluation of fiber-reinforced polymers. At present, the primary approach to improving the post-heat mechanical property of a material involves incorporating inorganic fillers; yet, the enhancement is limited, and is accompanied by a reduction in room-temperature performance and processability. This study prepares glass-fiber-reinforced composites with elevated mechanical properties after heat through utilizing two variants of epoxy resins modified with polysiloxane, phenolic resin, kaolin, and graphite. In comparison to the phenolic samples, the phenylpropylsiloxane-modified epoxy resulted in a 115% rise in post-heat flexural strength and a 70% increase in the room-temperature flexural strength of phenolic composites. On the other hand, dimethylsiloxane-modified epoxy leads to a 117% improvement in post-heat flexural strength but a 44% decrease in the room-temperature flexural strength of phenolic composites. Macroscopic/microscopic morphologies and a residual structure model of the composites after heat reveal that, during high temperature exposure, the pyrolysis products of polysiloxane promote interactions between carbon elements and fillers, thus preserving more residues and improving the dimensional stability as well as the density of materials. Consequently, a notable enhancement is observed in both the post-heat flexural strength and the mass of carbon residue after the incorporation of polysiloxane and fillers into the materials. The pyrolysis products of polysiloxane-modified epoxy play a vital role in enhancing the post-heat flexural strength by promoting carbon retention, carbon fixation, and interactions with fillers, offering novel pathways for the development of advanced composites with superior fire-resistance properties.

Background and aim: Direct posterior reduction and manipulation of the C1-2 joints, accompanied by placement of spacers, is the state-of-the-art technique for treating basilar invagination (BI) and atlantoaxial dislocation (AAD). The hindrance of occiput to reaching up to the true atlantoaxial facets (AAF) during the surgery remains challenging for cage placement. The aim of this study was to explore an objective and precise method of measuring the effect of the hindrance of occiput to reaching up to the true AAF and cage placement during surgery. Method: We collected the clinico-imaging data of 58 patients with BI and AAD (Group A) who underwent surgery in our hospital, and 78 control cohorts (Group B) were retrieved retrospectively. We measured facet-occiput slope angle (FOSA) in midsagittal CT. Patients were positioned prone for surgery based on preoperative flexion O–C2a, and access to the true AAF was observed intraoperatively. The cut-off value of FOSA for the feasibility of cage placement in BI and AAD patients was appointed when access to the true AAF was impossible due to the hindrance of occiput during surgery. Results: The cut-off value of FOSA for the feasibility of cage placement was 34o with an area under the curve AUC of 0.800 (95 % CI: 0.672–0.928, P 34o, reaching up to the true AAF and 3D-printed cage placement was impossible. FOSA was negative in Group A and positive in Group B, significantly larger in females compared to males in both groups and significantly larger postoperatively in Group A. Conclusion: FOSA can objectively measure the feasibility of cage placement when the patient is positioned prone per preoperative flexion O–C2a. A FOSA >34o is contraindication for cage placement.

The extensive utilization of zinc oxide nanoparticles in consumer products and the industry has led to their substantial entry into the soil through air and surface runoff transportation, which causes ecotoxicity in agro-ecosystems and detrimental effects on crop production. Nanobubbles (diameter size < 1 µm) have many advantages, such as a high surface area, rapid mass transfer, and long retention time. In this study, wheat seedlings were irrigated with a 500 mg L−1 zinc oxide nanoparticle solution delivered in the form of nanobubble watering (nanobubble-ZnO-NPs). We found that nanobubble watering improved the growth and nutrient status of wheat exposed to zinc oxide nanoparticles, as evidenced by increased total foliar nitrogen and phosphorus, along with enhanced leaf dry mass per area. This effect can be attributed to nanobubbles disassembling zinc oxide aggregates formed due to soil organic carbon, thereby mitigating nutrient absorption limitations in plants. Furthermore, nanobubbles improved the capability of soil oxygen input, leading to increased root activity and glycolysis efficiency in wheat roots. This work provides valuable insights into the influence of nanobubble watering on soil quality and crop production and offers an innovative approach for agricultural irrigation that enhances the effectiveness and efficiency of water application.

The team-adversary game simulates many real-world scenarios in which a team of agents competes cooperatively against an adversary. However, decision-making in this type of game is a big challenge since the joint action space of the team is combinatorial and exponentially related to the number of team members. It also hampers the existing equilibrium finding algorithms from solving team-adversary games efficiently. To solve this issue caused by the combinatorial action space, we propose a novel framework based on Counterfactual Regret Minimization (CFR) framework: CFR-MIX. Firstly, we propose a new strategy representation to replace the traditional joint action strategy by using the individual action strategies of all the team members, which can significantly reduce the strategy space. To maintain the cooperation between team members, a strategy consistency relationship is proposed. Then, we transform the consistency relationship of the strategy to the regret consistency for computing the equilibrium strategy with the new strategy representation under the CFR framework. To guarantee the regret consistency relationship, a product-form decomposition method over cumulative regret values is proposed. To implement this decomposition method, our CFR-MIX framework employs a mixing layer under the CFR framework to get the final decision strategy for the team, i.e., the Nash equilibrium strategy. Finally, we conduct experiments on games in different domains. Extensive results show that CFR-MIX significantly outperforms state-of-the-art algorithms. We hope it can help the team make decisions in large-scale team-adversary games.

In this work, living diatoms (LDT) were fed Eu3+-complex-polysaccharide aggregates (EIPA), and the living diatoms integrated-EIPAs (LDT-E) in a completely new UV downconversion biophotonic material. The EIPAs were embedded inside siliceous cell walls of living diatoms. When dried, these diatoms (LDT-Es) are luminescent at 612 nm, due to the Eu3+-complex. As UV-downconversion material, LDT-E absorbs UV light in the band of 250–400 nm and converts into useful visible light, thus contributing to UV-protection and potentially to photocurrent generation in photovoltaic devices. This downconversion material from living diatom retains the porous characteristics of the diatom siliceous cells, as well as the diatom organic components. Most important is the absence of a chemical process to generate this biomaterial from natural living diatoms, in order to obtain UV-protection and downconversion.

The shales in the 2nd Member of Permian Shanxi Formation (Shan2 Member) at the Daning-Jixian block at the eastern margin of the Ordos Basin were deposited in a marine–continental transitional environment. The Shan23 sub-member, which is the target interval for transitional shale gas exploration and development in China, is characterized by substantial shale thickness, few and thin interlayers. This interval, however, has been rarely investigated for reservoir characteristics, especially the controlling factors of high-quality reservoirs. In this paper, the reservoir characteristics and main controlling factors of high-quality reservoirs in Shan23 in the Daning-Jixian block were systematically discussed by organic geochemical analysis, mineral compositional analysis, and microscopic reservoir characterization. The results indicate that the lagoon facies shale interval in the upper part of the Shan23 sub-member has a high total organic carbon (TOC) content, a high brittle mineral content, and high BET surface area and BJH pore volume values, indicating that this is the sweet spot for shale gas exploration and development. Pore space in the Shan23 sub-member shale is dominated by clay mineral intercrystalline pores (76.9%), which also contains organic matter pores (18.7%). The SEM observations reveal that the mineral components are different in pore size distribution (PSD) and the organic matter pores and calcite dissolved pores are mainly in meso-scale, the pores in clay minerals and quartz are in meso- and macro-scale, while the pores in feldspar and pyrite exhibit a wide and uniform size distribution. The single-factor analysis shows that the clay mineral content is the dominant factor controlling pore development in the Shan23 sub-member transitional shales, the TOC content has a certain effect on pore development, and the kerogen type has no discernible effect on pore development.

Transit Signal Priority (TSP) is a system designed to grant right-of-way to buses, yet it can lead to delays for private vehicles. With the rapid advancement of network technology, self-driving buses have the capability to efficiently acquire road information and optimize the coordination between vehicle arrival and signal timing. However, the complexity of arterial intersections poses challenges for conventional algorithms and models in adapting to real-time signal priority. In this paper, a novel real-time signal-priority optimization method is proposed for self-driving buses based on the CACC model and the powerful deep Q-network (DQN) algorithm. The proposed method leverages the DQN algorithm to facilitate rapid data collection, analysis, and feedback in self-driving scenarios. Based on the arrival states of both the bus and private vehicles, appropriate actions are chosen to adjust the current-phase green time or switch to the next phase while calculating the duration of the green light. In order to optimize traffic balance, the reward function incorporates an equalization reward term. Through simulation analysis using the SUMO framework with self-driving buses in Zhengzhou, the results demonstrate that the DQN-controlled self-driving TSP optimization method reduces intersection delay by 27.77% and 30.55% compared to scenarios without TSP and with traditional active transit signal priority (ATSP), respectively. Furthermore, the queue length is reduced by 33.41% and 38.21% compared to scenarios without TSP and with traditional ATSP, respectively. These findings highlight the superior control effectiveness of the proposed method, particularly during peak hours and in high-traffic volume scenarios.

The Shanxi Formation Shan-2 Lower Sub-member transitional shale in the eastern Ordos Basin is characterized by high total organic carbon value, wide distribution, and large single-layer/cumulative thickness; it is the key target interval for transitional shale gas exploration. Based on drilling, logging, 3D seismic, and natural energy spectrum data, this study discusses the spatial distribution and controlling factors of complex lithology in the transitional shale stratum. Using the multi-lithological eigenvalue method, the lithologic differential distribution characteristics of the Shan-2 Lower Sub-member were identified through seismic attribute analysis and post-stack seismic parameter inversion. On this basis, the controlling factors of lithology spatial difference distribution are revealed by paleogeomorphology restoration, the sedimentary environmental indicator characteristics of shale, and seismic facies division. The results indicate that the lithology distribution of transitional shale strata in the Shan-2 Lower Sub-member is significantly different, and the geomorphological pattern of interjacent depressions and highlands lays a foundation for the formation of the sedimentary system. The environmental and energy differences of paleo-sedimentary water form a sedimentary environment with frequent facies transitions, which controls the distribution of sediments. Th/U and Th/K radios, which indicate redox conditions and sedimentary water energy during the early sedimentary stage of the Shan-2 Lower Sub-member, show that the geomorphic uplift area is characterized by a high-energy transitional environment of mainly developed sandstone and silty mudstone, while the depression is relatively low-energy under a brackish water reduction environment, mainly composed of both dark and carbonaceous shale. During the late sedimentary stage of the Shan-2 Lower Sub-member, when geomorphic control was weakened, it presented a shallow-water, high-energy, and oxidized transitional environment, with a lithology dominated by the complex interbedding of fine-grained sandstone, silty shale, shale, carbonaceous shale, and coal seam. Therefore, it is inferred that the Shan-2 Lower Sub-member may have developed as a barrier island-lagoon sedimentary model. The early depositional period was dominated by barrier island-lagoon-tidal flat depositional combination, thence the sedimentary environment gradually evolved into a tidal flat-swamp environment.

Based on core description, thin section identification, X-ray diffraction analysis, scanning electron microscopy, low-temperature gas adsorption and high-pressure mercury intrusion porosimetry, the shale lithofacies of Shan23 sub-member of Permian Shanxi Formation in the east margin of Ordos Basin was systematically analyzed in this study. The Shan23 sub-member has six lithofacies, namely, low TOC clay shale (C-L), low TOC siliceous shale (S-L), medium TOC siliceous shale (S-M), medium TOC hybrid shale (M-M), high TOC siliceous shale (S-H), and high TOC clay shale (C-H). Among them, S-H is the best lithofacies, S-M and M-M are the second best. The C-L and C-H lithofacies, mainly found in the upper part of Shan23 sub-member, generally developed in tide-dominated delta facies; the S-L, S-M, S-H and M-M shales occurring in the lower part of Shan23 sub-member developed in tide-dominated estuarine bay facies. The S-H, S-M and M-M shales have good pore structure and largely organic matter pores and mineral interparticle pores, including interlayer pore in clay minerals, pyrite intercrystalline pore, and mineral dissolution pore. C-L and S-L shales have mainly mineral interparticle pores and clay mineral interlayer pores, and a small amount of organic matter pores, showing poorer pore structure. The C-H shale has organic micro-pores and a small number of interlayer fissures of clay minerals, showing good micro-pore structure, and poor meso-pore and macro-pore structure. The formation of favorable lithofacies is jointly controlled by depositional environment and diagenesis. Shallow bay-lagoon depositional environment is conducive to the formation of type II2 kerogen which can produce a large number of organic cellular pores. Besides, the rich biogenic silica is conducive to the preservation of primary pores and enhances the fracability of the shale reservoir.

The marine–continental transitional Permian Shanxi Formation, which is located on the eastern margin of the Ordos Basin, is expected to be another set of economic gas shale for exploration and development in China. In this study, geochemical methods, low-pressure gas adsorption, high-pressure mercury intrusion, and field emission scanning electron microscopy (FE-SEM) imaging were performed to investigate the pore structure characteristics of the second member of the Permian Shanxi gas shale. The results show that the average TOC content of the shale is 5.90%, the average thermal maturity is 2.22% Ro, and these organic-rich shales are enriched in clay minerals. Mesopores are the predominant pore type in these shales, and have slit-like shapes. FE-SEM analysis reveals that the interparticle pores associated with clay minerals are more developed than organic-hosted pores, while a few SEM-invisible micropores may be related to the aromatic structures of kerogen in the shale. Pore development in the studied samples is influenced by the TOC and clay mineral contents. The development of organic-hosted pores in the shale shows obvious differences when compared to representative marine gas shales worldwide, which are ascribed to the differences in kerogen types. Nevertheless, the development of micropores and mesopores in the samples is comparable to that of marine shales, which indicates the presence of qualified space for gas storage in the studied shale. This study will provide crucial theoretical guidance for determining of sweet spots and for the further exploration and development of the transitional shale gas.

A reliable finite element procedure to simulate shear-dominated ductile fractures in large-scale, thin-walled steel structures is still evolving primarily due to the challenges in determining the failure criterion of metal materials under complex stress states. This paper aims to examine the accuracy of the modified Gurson–Tvergaard–Needleman (GTN) model considering the shear failure in simulating the ductile fracture of steel plate structures under quasi-static punch loading. The modified GTN damage models are performed by the ABAQUS user-defined material subroutine (VUMAT). The void-related parameters and shear damage parameters of Xue’s and the N-H modified GTN models are calibrated from test specimens with various geometries corresponding to different stress triaxiality and shearing conditions. The damage evolution associated with shearing of voids in the modified GTN models has strong influences on the stress triaxiality versus plastic strain under complex stress states, especially for the shear-dominated loading conditions. Based on the original GTN model, Xue’s and the N-H modified GTN model with calibrated material parameters, a numerical comparative study examines the ductile fracture of steel non-stiffened plates and stiffened plates under punch loading. Benchmarked against the experimental studies, the numerical simulations demonstrate that the shear-driven void evolution in the modified GTN model imposes significant effects on the load–displacement responses as well as the onset and extension of ductile fractures in steel plates under punch actions. The N-H modified model with calibrated shear damage parameters shows a better correlation with the ductile fractures in steel plates observed in the experiment than the original GTN model and Xue’s modified GTN model. As a result of this study, the modified GTN model considering shear action can be applied for practical applications in the crashworthiness assessment of ship collision and grounding.

The Atr-Check1 pathway is involved in cell cycle and the DNA damage response. Here, the authors show that the Atr-Check1 pathway can inhibit axon regeneration in response to Piezo-mediated mechanosensation, affecting functional recovery.

In filament wound composites, fiber bundles cross each other and form an undulating architecture, which may significantly affect the mechanical behavior of composites. In this study, the tensile mechanical behavior of filament wound laminates was studied experimentally and numerically, and the influences of the bundle thickness and winding angle on the mechanical behavior of the filament wound plates were also explored. In the experiments, tensile tests were carried out on filament wound plates and laminated plates. It was found that, compared to laminated plates, filament wound plates had lower stiffness, greater failure displacement, similar failure loads, and more obvious strain concentration areas. In numerical analysis, mesoscale finite element models, which take into account the fiber bundles’ undulating morphology, were created. The numerical predictions correlated well with the experimental ones. Further numerical studies have shown that the stiffness reduction coefficient of filament wound plates with a winding angle of ±55° decreased from 0.78 to 0.74 as the bundle thickness increased from 0.4 mm to 0.8 mm. The stiffness reduction coefficients of filament wound plates with wound angles of ±15°, ±25°, and ±45° were 0.86, 0.83, and 0.8, respectively.

To investigate the tribological performance of a copper alloy engine bearing under oil lubrication, seawater corrosion and dry sliding wear, three different PI/PAI/EP coatings consisting of 1.5 wt% Ce2O3, 2 wt% Ce2O3, 2.5 wt% Ce2O3 were designed, respectively. These designed coatings were prepared on the surface of CuPb22Sn2.5 copper alloy using a liquid spraying process. The tribological properties of these coatings under different working conditions were tested. The results show that the hardness of the coating decreases gradually with the addition of Ce2O3, and the agglomeration of Ce2O3 is the main reason for the decrease of hardness. The wear amount of the coating increases first and then decreases with the increase of Ce2O3 content under dry sliding wear. The wear mechanism is abrasive wear under the condition of seawater. The wear resistance of the coating decreases with the increase of Ce2O3 content. The wear resistance of the coating with 1.5 wt% Ce2O3 is the best under-seawater corrosion. Although Ce2O3 has corrosion resistance, the coating of 2.5 wt% Ce2O3 has the worst wear resistance under seawater conditions due to severe wear caused by agglomeration. Under oil lubrication conditions, the frictional coefficient of the coating is stable. The lubricating oil film has a good lubrication and protection effect.

Salt acclimation can promote the tolerance of wheat plants to the subsequent salt stress, which may be related to the responses of the photosynthetic apparatus. The chlorophyl (Chl) b-deficient mutant wheat ANK 32B and its wild type (WT) were firstly saltly acclimated with 30 mmol NaCl for 12 days, then subsequently subjected to 6-day salt stress (500 mmol NaCl). The ANK 32B mutant plants had lower Chl b concentration, which was manifested in the lower total Chl concentration, higher ratio of Chl a/b and in reduced photosynthetic activity (Pn). The effect of salt acclimation was manifested mainly after salt stress. Compared to non-acclimated plants, the salt acclimation increased the leaf water potential, osmotic potential (Ψo) and K concentration, while decreased the amount of Na+ and H2O2 in WT and ANK 32B under salt stress, except for Ψo in ANK 32B. In addition, the salt acclimation enhanced the APX (ascorbate peroxidase) activity by 10.55% and 33.69% in WT and ANK 32B under salt stress, respectively. Compared to the genotypes, under salt stress, the Ψo, Fv/Fm, Pn and gs of mutant plants were 5.60, 17.62, 46.73 and 26.41% lower than that of WT, respectively. These results indicated that although the salt acclimation could alleviate the negative consequences of salt stress, it is mainly manifested in the WT, and the ANK 32B plants had lower salt tolerance than WT plants, suggesting that lower Chl b concentration has a negative effect on the salt acclimation induced salt tolerance in wheat.

This work details a newly developed “sandwich” nanoplatform via neutravidin-biotin system for the detection and treatment of inflammation. First, biotinylated- and folate-conjugated optical imaging micelles targeted activated macrophages via folate/folate receptor interactions. Second, multivalent neutravidin proteins in an optimal concentration accumulated on the biotinylated macrophages. Finally, biotinylated anti-inflammatory drug-loaded micelles delivered drugs effectively at the inflammatory sites via a highly specific neutravidin-biotin affinity. Both in vitro and in vivo studies have shown that the “sandwich” pretargeting platform was able to diagnose inflammation by targeting activated macrophages as well as improve the therapeutic efficacy by amplifying the drug delivery to the inflamed tissue. The overall results support that our new pretargeting platform has the potential for inflammatory disease diagnosis and treatment.

Background In intensive and large-scale farms, abnormal estradiol levels in sows can cause reproductive disorders. The high incidence rate of reproductive disturbance will induce the elimination of productive sows in large quantities, and the poor management will bring great losses to the pig farms. The change in estradiol level has an important effect on follicular development and estrus of sows. To solve this practical problem and improve the productive capacity of sows, it is significant to further clarify the regulatory mechanism of estradiol synthesis in porcine granulosa cells (GCs). The most important function of granulosa cells is to synthesize estradiol. Thus, the studies about the complex transcriptome in porcine GCs are significant. As for precursor-messenger RNAs (pre-mRNAs), their post-transcriptional modification, such as alternative polyadenylation (APA) and alternative splicing (AS), together with long non-coding RNAs (lncRNAs), may regulate the functions of granulosa cells. However, the above modification events and their function are unclear within pig granulosa cells. Methods Combined PacBio long-read isoform sequencing (Iso-Seq) was conducted in this work for generating porcine granulosa cells’ transcriptomic data. We discovered new transcripts and possible gene loci via comparison against reference genome. Later, combined Iso-Seq data were adopted to uncover those post-transcriptional modifications such as APA or AS, together with lncRNA within porcine granulosa cells. For confirming that the Iso-Seq data were reliable, we chose four AS genes and analyzed them through RT-PCR. Results The present article illustrated that pig GCs had a complex transcriptome, which gave rise to 8,793 APA, 3,465 AS events, 703 candidate new gene loci, as well as 92 lncRNAs. The results of this study revealed the complex transcriptome in pig GCs. It provided a basis for the interpretation of the molecular mechanism in GCs.

To bolster freshwater supply, artificial groundwater recharge with recycled water has increasingly attracted research attentions and interests. However, artificial groundwater recharge has potential risks to groundwater quality, as recharge water disinfection is frequently used for pathogen inactivation and causes the concerns of disinfection by-products (DBPs). Colloid supplement is a good approach solving this problem, but its roles in mitigating DBPs remains unclear. In this study, we collected 20 groundwater and soil samples from a field-scale groundwater recharge project, and explored the impacts of silica colloids on chloroform migration and groundwater bacterial communities during the recharge process. Water physicochemical variables changed along the recharge time, and colloid supplement significantly reduced chloroform formation and slowed its migration in groundwater. Bacterial communities in groundwater, river water and recharge water were significantly different. Gammaproteobacteria in recharge water (71.7%) was more abundant than in river water (30.5%) and groundwater (33.5%), while Actinobacteria dominated groundwater (40.6%). After recharge, Gammaproteobacteria increased more with colloid supplement (75.7%) than without (52.6%), attributing to its dominance in soils (74.6%). Our results suggested more bacterial lineages released from soils into aquifer by silica colloid supplement, owing to the competitive adsorption encouraging microbial transfer, especially Gram-negative bacteria. Our findings unraveled the effects of colloid supplement on chloroform formation and migration during artificial groundwater recharge, which consequently altered groundwater bacterial communities, and offered valuable suggestions for the safety management of DBPs in aquifer recharge.

Abstract: The shales in the 2nd Member of Shanxi Formation in the east margin of the Ordos Basin were deposited in a marine-nonmarine transitional environment during the Permian. Based on the recent breakthroughs in the shale gas exploration and theoretical understandings on the shale gas of the study area, with a comparison to marine shale gas in the Sichuan Basin and marine-nonmarine transitional shale gas in the U.S., this study presents the geological characteristics and development potential of marine-nonmarine transitional gas in the study area. Four geological features are identified in the 2nd Member of the Shanxi Formation in the study area has: (1) stable sedimentary environment is conductive to deposition of widely distributed organic shale; (2) well-developed micro- and nano- scale pore and fracture systems, providing good storage capacity; (3) high content of brittle minerals such as quartz, leading to effectively reservoir fracturing; and (4) moderate reservoir pressure and relatively high gas content, allowing efficient development of shale gas. The 2nd Member of Shanxi Formation in the east margin of Ordos Basin is rich in shale gas resource. Three favorable zones, Yulin-Linxian, Shiloubei-Daning-Jixian, and Hancheng-Huangling are developed, with a total area of 1.28×104 km2 and resources between 1.8×1012 and 2.9×1012 m3, indicating a huge exploration potential. Tests of the 2nd Member of Shanxi Formation in vertical wells show that the favorable intervals have stable gas production and high reserves controlled by single well, good recoverability and fracability. This shale interval has sufficient energy, stable production capacity, and good development prospects, as evidenced by systematic well testing. The east margin of the Ordos Basin has several shale intervals in the Shanxi and Taiyuan formations, and several coal seams interbedded, so collaborative production of different types of natural gas in different intervals can be considered. The study results can provide reference for shale gas exploration and development and promote the rapid exploitation of shale gas in China.

Cartilage injury affects millions of people throughout the world, and at this time there is no cure. While transplantation of stem cells has shown some success in the treatment of injured cartilage, such treatment is limited by limited cell sources and safety concerns. To overcome these drawbacks, a microscaffolds system was developed capable of targeting, reducing the inflammatory response and recruiting endogenous progenitor cells to cartilage-defect. Erythropoietin (EPO)-loaded-hyaluronic acid (HA) microscaffolds (HA + EPO) were fabricated and characterized. HA-microscaffolds showed good cell-compatibility and could target chondrocytes via CD44 receptors. HA + EPO was designed to slowly release EPO while recruiting progenitor cells. Finally, the ability of HA + EPO to repair cartilage-defects was assessed using a rabbit model of full-thickness cartilage-defect. Our results showed that the intra-articular administration of EPO, HA, and EPO + HA reduced the number of inflammatory cells inside the synovial-fluid, while EPO + HA had the greatest anti-inflammatory effects. Furthermore, among all groups, EPO + HA achieved the greatest progenitor cell recruitment and subsequent chondrogenesis. The results of this work support that, by targeting and localizing the release of growth-factors, HA + EPO can reduce inflammatory responses and promote progenitor cells responses. This new platform represents an alternative treatment to stem-cell transplantation for the treatment of cartilage injury. Keywords: Hyaluronic acid microscaffolds, Erythropoietin, Progenitor cells recruitment, Chondrogenesis, Cartilage injury

Marine–continental transitional (hereinafter referred to as transitional) Permian shales are important targets for shale gas in China because of the considerable volumes of shale gas resources present in them. In this study, transitional shale samples from the Permian Shanxi Formation in the Daning–Jixian block along the eastern margin of the Ordos Basin were collected to investigate the effects of organic and inorganic compositions on the development of their pore structures through organic petrographic analysis, X–ray diffraction, scanning electron microscope (SEM) observation, gas (N2 and CO2) adsorption, high-pressure mercury injection (HPMI), and methane adsorption experiments. The organic petrographic analysis reveals that the Permian Shanxi shale comprises Type-II2-III kerogens, and the average vitrinite reflectance (Ro) is 2.3% at the overmature stage or in the dry gas window. The shale interval at the bottom of the lagoon facies is considered the most favorable interval throughout the entire section because of its high total organic carbon (TOC) content (4.19–43.9%; an average of 16.9%) and high brittle mineral content (38.3–73.2%; an average of 55.8%). N2 and CO2 gas adsorption and HPMI tests reveal the pore size distribution characteristics of the shale. The full pore size distribution by the gas adsorption and HPMI test reveals that micropores (

Shanxi Formation Shan 23 Submember transitional shale in Eastern Ordos Basin is characterized by high TOC value, wide distribution, and large single-layer/cumulative thickness. In this study, based on section division of Shan 23 Submember, petrographic, mineralogical, and high-resolution geochemical analyses were integrated to reveal sedimentary environment, detrital influx, paleoclimate, paleosalinity, and paleoredox conditions. Results indicate that Shan 23 Submember is divided into four sections (Shan 23-1, Shan 23-2, Shan 23-3, and Shan 23-4). The upper part of the Shan 23-1 section is dominated by bay facies, which is characterized by high TOC value (2.75%–10.96%, avg. 6.98%), low detrital influx proxies (Zr, 97–527 ppm, avg. 310 ppm; Ti, 1985–7591 ppm, avg. 3938 ppm), relatively dry paleoclimate condition (CIA*, 41.96–92.58, avg. 75.55; Sr/Cu, 6.23–14.49, avg. 8.87), high paleosalinity proxies (Sr/Ba, 0.39–1.29, avg. 0.62), and relatively anoxic reduction condition (UEF, 0.83–3.00, avg. 1.67; MoEF, 3.95–27.00, avg. 15.56). By comparison, the other three sections are dominated by a combination of transitional facies, including barrier island, lagoon, tidal flat, and swamp. In this interval, shale is deposited in lagoon facies. The paleoclimate gradually tends to be warm and humid, which results in increasing the chemical weathering intensity. Meanwhile, the detrital influx increases, and the paleoredox condition tends to be oxic. The above conditions are not conducive to the preservation of organic matter in lagoon facies shale.

Solar flares are solar storm events driven by the magnetic field in the solar activity area. Solar flare, often associated with solar proton event or CME, has a negative impact on ratio communication, aviation, and aerospace. Therefore, its forecasting has attracted much attention from the academic community. Due to the limitation of the unbalanced distribution of the observation data, most techniques failed to effectively learn complex magnetic field characteristics, leading to poor forecasting performance. Through the statistical analysis of solar flare magnetic map data observed by SDO/HMI from 2010 to 2019, we find that unsupervised clustering algorithms have high accuracy in identifying the sunspot group in which the positive samples account for the majority. Furthermore, for these identified sunspot groups, the ensemble model that integrates the capability of boosting and convolutional neural network (CNN) achieves high-precision prediction of whether the solar flares will occur in the next 48 hours. Based on the above findings, a two-stage solar flare early warning system is established in this paper. The F1 score of our method is 0.5639, which shows that it is superior to the traditional methods such as logistic regression and support vector machine (SVM).

Thin-ply composite failure modes also significantly differ from conventional ply composite failure modes, with the final failure mechanism switching from irregular progressive failure to direct fracture characterized by a uniform fracture with the reduction of the ply thickness. When open holes and bolt joints are involved, thin-ply-laminated composites exhibit more complex stress states, damage evolution, and failure modes. Compared to the experimental study of thin-ply-laminated composite-bolted joints, there are few reports about numerical analysis. In order to understand the damage evolution and failure mechanism of thin-ply-laminated composites jointed by single-lap bolt, a progressive damage model based on three-dimensional (3D) LaRC failure criterion combined with cohesive element is constructed. Through an energy-based damage evolution method, this model can capture some significant mechanical characteristics in thin-ply-laminated structures, such as the in situ effect, delamination inhibition, and fiber compressive kinking failure. The comparisons between the numerical predictions and experimental observations are made to verify the accuracy of the proposed model. It is found that the predicted stress-displacement curves, failure modes, damage morphologies, etc., are consistent with the experimental results, indicating that the presented progressive damage analysis method displays excellent accuracy. The predicted stress at the onset of delamination is 50% higher than that of the conventional thick materials, which is also consistent with experimental results. Moreover, the numerical model provides evidence that the microstructure of thin-ply-laminated composite performs better in uniformity, which is more conducive to inhibiting the intra-layer damage and the expansion of delamination damage between layers. This study on the damage inhibition mechanism of thin-ply provides a potential analytical tool for evaluating damage tolerance and bearing capabilities in thin-ply-laminated composite-bolted joints.

The liver kinase B1 (LKB1), also known as serine/threonine kinase 11 (STK11) and Par-4 in C. elegans, has been identified as a master kinase of AMPKs and AMPK-related kinases. LKB1 plays a crucial role in cell growth, metabolism, polarity, and tumor suppression. By interacting with the downstream signals of SAD, NUAK, MARK, and other kinases, LKB1 is critical to regulating neuronal polarization and axon branching during development. It also regulates Schwann cell function and the myelination of peripheral axons. Regulating LKB1 activity has become an attractive strategy for repairing an injured nervous system. LKB1 upregulation enhances the regenerative capacity of adult CNS neurons and the recovery of locomotor function in adult rodents with CNS axon injury. Here, we update the major cellular and molecular mechanisms of LKB1 in regulating neuronal polarization and neural development, and the implications thereof for promoting neural repair, axon regeneration, and functional recovery in adult mammals.

An advanced modeling strategy for notched ceramic matrix composite coupons with patch reinforcement was proposed to investigate the failure mechanisms. This model considered the tailored fiber–placed (TFP) yarn details obtained from the design phase and the embedded element concept which was used to successfully overcome the meshing difficulties. Inter-ply “glue” layers were simulated using the surface-based contact cohesive element method, so the delamination due to interfacial material discontinuity and damage can be well reproduced and analyzed. For composite ply, the energy-based composite progressive damage model that is independent of the mesh size was applied. Virtual test campaign was performed with a variety of geometrical and material parameters, and the damage and failure mechanisms based on the stress analysis can be revealed to support the design optimization of patch reinforcement.

Promoting the combination of robust regeneration of damaged axons and synaptic reconnection of these growing axon populations with appropriate neuronal targets represents a major therapeutic goal following spinal cord injury (SCI). A key impediment to achieving this important aim includes an intrinsic inability of neurons to extend axons in adult CNS, particularly in the context of the chronically-injured spinal cord. We tested whether an inhibitory peptide directed against phosphatase and tensin homolog (PTEN: a central inhibitor of neuron-intrinsic axon growth potential) could restore inspiratory diaphragm function by reconnecting critical respiratory neural circuitry in a rat model of chronic cervical level 2 (C2) hemisection SCI. We found that systemic delivery of PTEN antagonist peptide 4 (PAP4) starting at 8 weeks after C2 hemisection promoted substantial, long-distance regeneration of injured bulbospinal rostral Ventral Respiratory Group (rVRG) axons into and through the lesion and back toward phrenic motor neurons (PhMNs) located in intact caudal C3-C5 spinal cord. Despite this robust rVRG axon regeneration, PAP4 stimulated only minimal recovery of diaphragm function. Furthermore, re-lesion through the hemisection site completely removed PAP4-induced functional improvement, demonstrating that axon regeneration through the lesion was responsible for this partial functional recovery. Interestingly, there was minimal formation of putative excitatory monosynaptic connections between regrowing rVRG axons and PhMN targets, suggesting that (1) limited rVRG-PhMN synaptic reconnectivity was responsible at least in part for the lack of a significant functional effect, (2) chronically-injured spinal cord presents an obstacle to achieving synaptogenesis between regenerating axons and post-synaptic targets, and (3) addressing this challenge is a potentially-powerful strategy to enhance therapeutic efficacy in the chronic SCI setting. In conclusion, our study demonstrates a non-invasive and transient pharmacological approach in chronic SCI to repair the critically-important neural circuitry controlling diaphragmatic respiratory function, but also sheds light on obstacles to circuit plasticity presented by the chronically-injured spinal cord.

Carbon fiber reinforced plastic (CFRP) is the main material of aircraft skin. Preparing superhydrophobic anti-icing/deicing surface on the CFRP is of great importance for aircraft flight safety. In this work, a variety of multi-scale micro-nano structures were imprinted on CFRP by femtosecond laser processing, and a transition from hydrophilic to superhydrophobic CFRP was realized. After being optimized by different geometries and laser conditions, the water contact angle, which is tested at 24.3 °C and 34% humidity, increased from 88 ± 2° (pristine) to 149 ± 3° (100 μm groove) and 153 ± 3° (80 μm grid). A further anti-icing test at −10 °C (measured on the cooling platform) and 28% humidity showed that the freezing time was increased from 78 ± 10 s (pristine) to 282 ± 25 s (80 μm grid). Most importantly, the tensile tests showed that the femtosecond laser processing method did not deteriorate the mechanical properties of CFRP. This work provides great significance for aircraft passive deicing technology.

Paralysis following spinal cord injury (SCI) is due to failure of axonal regeneration. It is believed that axon growth is inhibited by the presence of several types of inhibitory molecules in central nervous system (CNS), including the chondroitin sulfate proteoglycans (CSPGs). Many studies have shown that digestion of CSPGs with chondroitinase ABC (ChABC) can enhance axon growth and functional recovery after SCI. However, due to the complexity of the mammalian CNS, it is still unclear whether this involves true regeneration or only collateral sprouting by uninjured axons, whether it affects the expression of CSPG receptors such as protein tyrosine phosphatase sigma (PTPσ), and whether it influences retrograde neuronal apoptosis after SCI. In the present study, we assessed the roles of CSPGs in the regeneration of spinal-projecting axons from brainstem neurons, and in the process of retrograde neuronal apoptosis. Using the fluorochrome-labeled inhibitor of caspase activity (FLICA) method, apoptotic signaling was seen primarily in those large, individually identified reticulospinal (RS) neurons that are known to be “bad-regenerators.” Compared to uninjured controls, the number of all RS neurons showing polycaspase activity increased significantly at 2, 4, 8, and 11 weeks post-transection (post-TX). ChABC application to a fresh TX site reduced the number of polycaspase-positive RS neurons at 2 and 11 weeks post-TX, and also reduced the number of active caspase 3-positive RS neurons at 4 weeks post-TX, which confirmed the beneficial role of ChABC treatment in retrograde apoptotic signaling. ChABC treatment also greatly promoted axonal regeneration at 10 weeks post-TX. Correspondingly, PTPσ mRNA expression was reduced in the perikaryon. Previously, PTPσ mRNA expression was shown to correlate with neuronal apoptotic signaling at 2 and 10 weeks post-TX. In the present study, this correlation persisted after ChABC treatment, which suggests that PTPσ may be involved more generally in signaling axotomy-induced retrograde neuronal apoptosis. Moreover, ChABC treatment caused Akt activation (pAkt-308) to be greatly enhanced in brain post-TX, which was further confirmed in individually identified RS neurons. Thus, CSPG digestion not only enhances axon regeneration after SCI, but also inhibits retrograde RS neuronal apoptosis signaling, possibly by reducing PTPσ expression and enhancing Akt activation.

Chondroitin sulfate proteoglycans (CSPGs), up-regulated in and around the lesion after traumatic spinal cord injury (SCI), are key extracellular matrix inhibitory molecules that limit axon growth and consequent recovery of function. CSPG-mediated inhibition occurs via interactions with axonal receptors, including leukocyte common antigen- related (LAR) phosphatase. We tested the effects of a novel LAR inhibitory peptide in rats after hemisection at cervical level 2, a SCI model in which bulbospinal inspiratory neural circuitry originating in the medullary rostral ventral respiratory group (rVRG) becomes disconnected from phrenic motor neuron (PhMN) targets in cervical spinal cord, resulting in persistent partial-to-complete diaphragm paralysis. LAR peptide was delivered by a soaked gelfoam, which was placed directly over the injury site immediately after C2 hemisection and replaced at 1 week post-injury. Axotomized rVRG axons originating in ipsilateral medulla or spared rVRG fibers originating in contralateral medulla were separately assessed by anterograde tracing via AAV2-mCherry injection into rVRG. At 8 weeks post-hemisection, LAR peptide significantly improved ipsilateral hemidiaphragm function, as assessed in vivo with electromyography recordings. LAR peptide promoted robust regeneration of ipsilateral-originating rVRG axons into and through the lesion site and into intact caudal spinal cord to reach PhMNs located at C3-C5 levels. Furthermore, regenerating rVRG axons re-established putative monosynaptic connections with their PhMNs targets. In addition, LAR peptide stimulated robust sprouting of both modulatory serotonergic axons and contralateral-originating rVRG fibers within the PhMN pool ipsilateral/caudal to the hemisection. Our study demonstrates that targeting LAR-based axon growth inhibition promotes multiple forms of respiratory neural circuit plasticity and provides a new peptide-based therapeutic strategy to ameliorate the devastating respiratory consequences of SCI.

Warm compression tests were carried out on low carbon and low alloy steel at temperatures of 600–850 °C and stain rates of 0.01–10 s−1. The evolution of microstructure and texture was studied using a scanning electron microscope and electron backscattered diffraction. The results indicated that cementite spheroidization occurred and greatly reduced at 750 °C due to a phase transformation. Dynamic recrystallization led to a transition from {112} texture to {111} texture. Below 800 °C, the intensity and variation of texture with deformation temperature is more significant than that above 800 °C. The contents of the {111} texture and {111} texture were equivalent above 800 °C, resulting in the better uniformity of γ-fiber texture. Nucleation of //ND-oriented grains increased, leading to the strengthening of //ND texture. Microstructure analysis revealed that the uniform and refined grains can be obtained after deformation at 800 °C and 850 °C. The texture variation reflected the fact that 800 °C was the critical value for temperature sensitivity of warm deformation. At a large strain rate, the lowest dislocation density appeared after deformation at 800 °C. Therefore, 800 °C is a suitable temperature for the warm forming application, where the investigated material is easy to deform and evolves into a uniform and refined microstructure.

Axon growth inhibitors generated by reactive glial scars play an important role in failure of axon regeneration after CNS injury in mature mammals. Among the inhibitory factors, chondroitin sulfate proteoglycans (CSPGs) are potent suppressors of axon regeneration and are important molecular targets for designing effective therapies for traumatic brain injury or spinal cord injury (SCI). CSPGs bind with high affinity to several transmembrane receptors, including two members of the leukocyte common antigen related (LAR) subfamily of receptor protein tyrosine phosphatases (RPTPs). Recent studies demonstrate that multiple intracellular signaling pathways downstream of these two RPTPs mediate the growth-inhibitory actions of CSPGs. A better understanding of these signaling pathways may facilitate development of new and effective therapies for CNS disorders characterized by axonal disconnections. This review will focus on recent advances in the downstream signaling pathways of scar-mediated inhibition and their potential as the molecular targets for CNS repair.