12 results on '"Shawn Jackson"'
Search Results
2. 4-H GROW: A Path for a Successful 4-H in School Program
- Author
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Michael Shawn Jackson and Andrew Toelle
- Subjects
4-H ,4-H in School ,4-H School Enrichment ,Agriculture (General) ,S1-972 ,Plant culture ,SB1-1110 ,Biology (General) ,QH301-705.5 - Abstract
4-H in School programs can reach large groups of youth in a narrow age range, yet they are difficult for many 4-H agents to implement. Building on Ask IFAS publication 4H324, the GROW model is a practical guide for agents to follow to increase the likelihood of beginning and sustaining a successful 4-H in School program. This model addresses who agents can reach out to when in the initial phases of beginning a 4-H in School program and steps to follow as it is being developed. The model also outlines an organizational plan to eliminate miscommunication and unexpected mishaps by offering a clear understanding of expectations for all participants involved. Finally, the model identifies tips to make a school enrichment program operate smoothly based on feedback from teachers and 4-H Agents with experience working with 4-H school enrichment programs.
- Published
- 2023
3. Adaptive multilevel median filtering of image sequences.
- Author
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Andreas E. Savakis and Shawn Jackson
- Published
- 2005
- Full Text
- View/download PDF
4. Novel Design for a Simple and Affordable End-Effector for an Upper-Limb Prosthesis
- Author
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Victoria Volk, Jodi Prosise, and Shawn Jackson
- Subjects
Computer science ,law ,business.industry ,medicine.medical_treatment ,medicine ,Upper limb prosthesis ,Customer requirements ,Robot end effector ,business ,Prosthesis ,Cleanability ,Computer hardware ,law.invention - Abstract
An affordable prosthetic arm was designed to be used in underprivileged areas to allow more upper-limb amputees to have access to prosthetics that can help them regain their independence. The primary user of the prosthesis will be the amputee(s) who receive the device; beneficiaries include the amputee’s family and the community involved in the manufacturing of future prostheses. The customer requirements include cost-effectiveness, the ability to withstand humidity, cleanability, low maintenance, long life, lightweight, and the ability to perform simple tasks independently. A new end-effector was created that meets the range-of-motion required for the user to perform his/her required tasks. The end-effector is comprised of a PVC cap, PVC coupling, wooden ball holding the tool, and two wooden stop rings. The device screws onto the forearm PVC pipe and locks into place. Four end-effectors were created: toothbrush, fork, spoon, and pen. A change-out station was created to allow the user to independently attach and detach the different end-effectors. The total cost of the device was $20.66.
- Published
- 2017
5. The zebrafish galectins Drgal1-L2 and Drgal3-L1 bind in vitro to the infectious hematopoietic necrosis virus (IHNV) glycoprotein and reduce viral adhesion to fish epithelial cells
- Author
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Chiguang Feng, Stuart M. Haslam, Mihai Nita-Lazar, Vikram N. Vakharia, Hafiz Ahmed, Anne Dell, Barbara Giomarelli, Shawn Jackson, Chinnarajan Ravindran, Gerardo R. Vasta, Nuria González-Montalbán, Justin Mancini, Arun Ammayappan, Ana de las Heras-Sánchez, Gang Wu, and Biotechnology and Biological Sciences Research Council (BBSRC)
- Subjects
0301 basic medicine ,DIFFERENTIAL RECOGNITION ,Viral Envelope Proteins ,ADAPTIVE IMMUNITY ,MOLECULAR CHARACTERIZATION ,Zebrafish ,Cells, Cultured ,chemistry.chemical_classification ,Infectivity ,Novirhabdovirus ,DANIO-RERIO ,Virulence ,biology ,Cell biology ,1107 Immunology ,Host-Pathogen Interactions ,Galectin ,ANGUILLA-JAPONICA ,PERKINSUS-MARINUS ,Life Sciences & Biomedicine ,Infectious hematopoietic necrosis virus ,animal structures ,Galectins ,Recombinant Fusion Proteins ,Immunology ,Viral adhesion ,Virus Attachment ,IHNV envelope glycoprotein ,Article ,03 medical and health sciences ,Chimera (genetics) ,Rhabdoviridae Infections ,Animals ,Infectious hematopoietic necrosis virus (IHNV) ,Science & Technology ,030102 biochemistry & molecular biology ,OYSTER CRASSOSTREA-VIRGINICA ,Epithelial Cells ,Zebrafish Proteins ,Rhabdoviridae ,SKIN MUCUS ,biology.organism_classification ,Virology ,030104 developmental biology ,chemistry ,MONOCLONAL-ANTIBODIES ,HOST-CELLS ,Glycoprotein ,Zoology ,Developmental Biology - Abstract
The infectious hematopoietic necrosis virus (IHNV; Rhabdoviridae, Novirhabdovirus) infects teleost fish, such as salmon and trout, and is responsible for significant losses in the aquaculture industry and in wild fish populations. Although IHNV enters the host through the skin at the base of the fins, the viral adhesion and entry mechanisms are not fully understood. In recent years, evidence has accumulated in support of the key roles played by protein-carbohydrate interactions between host lectins secreted to the extracellular space and virion envelope glycoproteins in modulating viral adhesion and infectivity. In this study, we assessed in vitro the potential role(s) of zebrafish (Danio rerio) proto type galectin-1 (Drgal1-L2) and a chimera galectin-3 (Drgal3-L1) in IHNV adhesion to epithelial cells. Our results suggest that the extracellular Drgal1-L2 and Drgal3-L1 interact directly and in a carbohydrate-dependent manner with the IHNV glycosylated envelope and glycans on the epithelial cell surface, significantly reducing viral adhesion.
- Published
- 2016
6. High-Dose Continuous Infusion Plus Pulse Interleukin-2 and Famotidine in Melanoma
- Author
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Nawazish Khan, Shawn Jackson, Mikhail Vinogradov, W. Chris Taylor, Walter D.Y. Quan, and Maria Ramirez
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Adult ,Male ,Interleukin 2 ,Cancer Research ,Lung Neoplasms ,Skin Neoplasms ,Lymphocytosis ,Continuous infusion ,Immunology ,Antineoplastic Agents ,Bone Neoplasms ,chemical and pharmacologic phenomena ,Pharmacology ,Bolus (medicine) ,In vivo ,medicine ,Humans ,Immunology and Allergy ,Radiology, Nuclear Medicine and imaging ,Infusions, Intravenous ,Pulse ,Melanoma ,Aged ,Lymphokine-activated killer cell ,Pulse (signal processing) ,business.industry ,Liver Neoplasms ,General Medicine ,Middle Aged ,Famotidine ,medicine.disease ,Oncology ,Interleukin-2 ,Drug Therapy, Combination ,Female ,Lymph Nodes ,medicine.symptom ,business ,T-Lymphocytes, Cytotoxic ,medicine.drug - Abstract
High-dose, continuous infusion interleukin-2 (IL-2) regimens generate greater Lymphokine Activated Killer cell (LAK) cytotoxicity in vitro and a higher rebound lymphocytosis in vivo than do bolus IL-2 regimens. Lymphocytes initially activated by continuous infusion IL-2 then subsequently pulsed with IL-2 have increased cytotoxicity against cancer cells. Famotidine may enhance the lysis of tumors by cytotoxic lymphocytes. Fourteen patients with melanoma were treated with famotidine 20 mg intravenously twice per day and continuous infusion IL-2 (18 MIU/sq m/24 hours) for 72 hours, followed by a 24-hour rest, then IL-2 18 MIU/sq m over 15-30 minutes for 1 dose (12 patients) or daily for 3 doses (2 patients). Most common toxicities were fever, nausea/emesis, hypophosphatemia, hypomagnesemia, and rigors. Nine partial responses (64% response rate; 95% Confidence Interval: 39%-84%) have been seen. Median survival has not been reached at greater than 10 months. Two patients responding to therapy showed an increase in detectable CD 56(+) cells in serial subcutaneous or lymph node biopsies, while 1 patient undergoing progression of disease had no such infiltrate. High-dose, 72-hour continuous infusion plus pulse interleukin-2 with famotidine has activity in melanoma. CD 56(+) cells may play a role in responding patients.
- Published
- 2004
7. Immune Thrombocytopenic Purpura as the Presenting Feature of Primary Small Bowel Adenocarcinoma
- Author
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Seth J Page, Elie Chalhoub, Shawn Jackson, and Eric R Wiedower
- Subjects
medicine.medical_specialty ,Gastrointestinal tumors ,business.industry ,Incidence (epidemiology) ,Small bowel adenocarcinoma ,Malignancy ,medicine.disease ,Gastroenterology ,Thrombocytopenic purpura ,Immune system ,immune system diseases ,Feature (computer vision) ,hemic and lymphatic diseases ,Internal medicine ,Medicine ,business - Abstract
Introduction • Small bowel adenocarcinoma is a rare malignancy with only 6,969 cases reported annually in the US, accounting for approximately 2% of gastrointestinal tumors and less than 0.4% of all malignancies. • Immune thrombocytopenic purpura (ITP) is a common hematological disorder with an incidence of two cases per 100,000 people. While ITP has many clinical manifestations, it uncommonly is the presenting feature of a malignancy.
- Published
- 2011
8. Ontogenic analysis of Xenopus γδ T cells: expressed T cell receptor (TCR) γ and δ chains and IL-17 family members (160.8)
- Author
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Shawn Jackson, Ada Undieh, Harold Steiner, Zuly Parra, Robert Miller, Yuko Ohta, and Martin Flajnik
- Subjects
Immunology ,Immunology and Allergy - Abstract
γδ TCR that have structural differences from conventional γδ TCR in mammals have been observed in non-eutherian vertebrates. Three different types of Vδ regions are present in Xenopus tropicalis, similar to Vδ, Vα, and VHδ (derived from the V regions of Ig H chains). Semi-quantitative PCR analyses in adult and larval Xenopus laevis show that expression levels of TCRδC and TCRγC, as well as of TCRδV and TCRγV repertoires, vary between tissues. Comparative analyses of adult and larval CDR3 sequences suggest ontogenic-, tissue- and perhaps V family-specific expression. We have detected changes at the VDJ junctions including N-nucleotide addition, nucleotide trimming, and homologous recombination events. γδ T cell populations in mammals differentially express either IL-17 or IFNγ. In mammals, six IL-17 forms have been described (A-E), the least well characterized of which is IL-17D. Most non-mammalian IL-17 homologs, including those in chicken, fish, lamprey, nematode, oyster, sea urchin, and tunicate, and especially, Xenopus, are the IL-17D form. We have cloned two types of IL-17A/F, IL-17B, and IL-17D from X. laevis. Semi-quantitative PCR analyses in adult and larval Xenopus show that the expression of these IL-17 family members vary during ontogeny and between tissues. Study of γδ TCR and IL-17 family expression in Xenopus will illuminate evolutionary and developmental changes in γδ T cell antigen recognition and effector strategies.
- Published
- 2012
9. Evolution of the T cell receptor: expression of non-conventional TCRδ chains in Xenopus (170.7)
- Author
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Shawn Jackson, Ada Undieh, Harold Steiner, Zuly Parra, Robert Miller, Yuko Ohta, and Martin Flajnik
- Subjects
Immunology ,Immunology and Allergy - Abstract
γδ T cell receptors (TCR) that have structural differences from conventional γδ T cells in mammals have been observed in non-eutherian vertebrates. Three different types of V regions are present in the frog Xenopus tropicalis genomic TCRδ locus (Eur. J. Immunol. 40: 2319, 2010), most similar to Vδ, Vα, and VHδ (evolutionarily derived from the V regions of Ig H chains). We have begun a survey of the tissue distribution of TCRδ expression in X. laevis tadpoles and adults by 5’ RACE PCR. The deduced amino acid sequences predicted from X. tropicalis genome sequences were aligned with those from X. laevis TCRδ V RACE clones, revealing similar Vδ, Vα, and VHδ families in X. laevis. Analyses of the TCRδ RACE nucleotide sequences have identified the putative D and J gene segments in X. laevis as well. Although we find representatives of all TCRδ V sub-families in all tested adult tissues, the distribution of the data suggests that the expressed repertoire is skewed in some tissues, especially in the skin, where a majority of recovered sequences were of the Vα type. Interestingly, analyses of the Vδ type indicate a lack of N-nucleotide addition in the CDR3 in adults, suggestive of a population of T cells that is generated during larval life that persists through metamorphosis. Continued study of these unique TCR in Xenopus will illuminate apparent plasticity in TCR evolution, and shed light on how γδ TCR recognize antigen in all vertebrates.
- Published
- 2011
10. Galectin binding to and expression modulation by viruses in zebrafish (89.51)
- Author
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Shawn Jackson, Ana de las Heras Sanchez, Hafiz Ahmed, Arun Ammayappan, Vikram Vakharia, and Gerardo Vasta
- Subjects
Immunology ,Immunology and Allergy - Abstract
The galectin family of β-galactoside-binding proteins is evolutionarily conserved. Galectins are known to interact with and affect the infectivity and/or pathogenicity of several viruses. We hypothesize that galectin interactions with viruses influence anti-viral immune responses. We investigate the interactions of zebrafish galectins with the glycosylated (G) protein of the rhabdovirus IHNV. We (a) characterized the carbohydrates present on IHNV proteins, and (b) identified direct interactions between zebrafish galectins (Drgal) and IHNV. We assessed the effects of immune challenge on (c) galectin and (d) cytokine expression in zebrafish. Several IHNV proteins exhibit complex glycosylation patterns. One or multiple IHNV proteins exhibit terminally linked mannose, sialic acid terminally linked to galactose, galactose-β(1-3)-GalNAc, sialic acid terminally linked to galactose or GalNAc, and galactose-β(1-4)-GlcNAc. The proto type Drgal1-L2, the chimera type Drgal3-L1 and the tandem-repeat type Drgal9-L2 all appear to bind to one or more IHNV proteins. All tested Drgal appear to bind to the IHNV G protein. ZFL cells endogenously express mRNA encoding Drgal1-L2, -L3, and -L4, Drgal3-L1 and -L2, and Drgal9-L1 mRNA. Drgal mRNA expression in ZFL cells was not induced by treatment with polyI:C. ZFL cells were found to endogenously express mRNA encoding Mx, TNFα, and IFNαβ. Treatment of these cells with polyI:C induced the expression of mRNA encoding IL-10 and IFNγ2.
- Published
- 2010
11. Galectin expression and modulation by viruses in fish cell lines (89.54)
- Author
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Shawn Jackson, Ana de las Heras Sanchez, Hafiz Ahmed, Vikram Vakharia, and Gerardo Vasta
- Subjects
Immunology ,Immunology and Allergy - Abstract
The galectin family of β-galactoside-binding proteins is evolutionarily conserved. In homeotherm vertebrates, galectin-virus interactions can affect viral infectivity and/or pathogenicity. We hypothesize that in teleost fish, galectin interactions with viral proteins modulate viral infectivity. We examined fish cell lines for expression of galectin-like molecules, and assessed changes in expression of identified galectins as well as of cytokines in response to viral infection. Minnow (EPC), trout (RTG-2), bluegill (BF-2), salmon (CHSE-214, ASK), zebrafish (ZFL), and catfish (COO, 42TA, G14D, 28S.3, 1G8, and 3B11) cells were tested. A proto-type galectin-like protein is expressed in EPC, RTG-2, and ZFL cell lines, and a chimera-type in EPC and RTG-2 cell lines. All tested cell lines express mRNA that exhibits high conservation with the zebrafish Drgal1-L2 proto-type galectin. Ten of the 12 cell lines express Drgal1-L3-like mRNA, 7 express Drgal1-L1-like mRNA, and 6 express Drgal1-L4-like mRNA. With the exception of 3B11, all tested cell lines express Drgal3-L1-like mRNA. Eight of the 12 express Drgal3-L2-like mRNA. Two of the 12 cell lines expressed Drgal9-like mRNA. Following infection of EPC cells with IHNV or IPNV, we observe modulations in expression of proto-type and tandem-repeat-type galectin-like molecules. We see modulations in expression of various cytokines, including type I and II interferons and TNF family members.
- Published
- 2010
12. High-Dose Continuous Infusion Plus Pulse Interleukin-2 and Famotidine in Melanoma.
- Author
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Walter Quan, Maria Ramirez, W. Chris Taylor, Mikhail Vinogradov, Nawazish Khan, and Shawn Jackson
- Published
- 2004
- Full Text
- View/download PDF
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