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The zebrafish galectins Drgal1-L2 and Drgal3-L1 bind in vitro to the infectious hematopoietic necrosis virus (IHNV) glycoprotein and reduce viral adhesion to fish epithelial cells
- Source :
- Developmental & Comparative Immunology. 55:241-252
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- The infectious hematopoietic necrosis virus (IHNV; Rhabdoviridae, Novirhabdovirus) infects teleost fish, such as salmon and trout, and is responsible for significant losses in the aquaculture industry and in wild fish populations. Although IHNV enters the host through the skin at the base of the fins, the viral adhesion and entry mechanisms are not fully understood. In recent years, evidence has accumulated in support of the key roles played by protein-carbohydrate interactions between host lectins secreted to the extracellular space and virion envelope glycoproteins in modulating viral adhesion and infectivity. In this study, we assessed in vitro the potential role(s) of zebrafish (Danio rerio) proto type galectin-1 (Drgal1-L2) and a chimera galectin-3 (Drgal3-L1) in IHNV adhesion to epithelial cells. Our results suggest that the extracellular Drgal1-L2 and Drgal3-L1 interact directly and in a carbohydrate-dependent manner with the IHNV glycosylated envelope and glycans on the epithelial cell surface, significantly reducing viral adhesion.
- Subjects :
- 0301 basic medicine
DIFFERENTIAL RECOGNITION
Viral Envelope Proteins
ADAPTIVE IMMUNITY
MOLECULAR CHARACTERIZATION
Zebrafish
Cells, Cultured
chemistry.chemical_classification
Infectivity
Novirhabdovirus
DANIO-RERIO
Virulence
biology
Cell biology
1107 Immunology
Host-Pathogen Interactions
Galectin
ANGUILLA-JAPONICA
PERKINSUS-MARINUS
Life Sciences & Biomedicine
Infectious hematopoietic necrosis virus
animal structures
Galectins
Recombinant Fusion Proteins
Immunology
Viral adhesion
Virus Attachment
IHNV envelope glycoprotein
Article
03 medical and health sciences
Chimera (genetics)
Rhabdoviridae Infections
Animals
Infectious hematopoietic necrosis virus (IHNV)
Science & Technology
030102 biochemistry & molecular biology
OYSTER CRASSOSTREA-VIRGINICA
Epithelial Cells
Zebrafish Proteins
Rhabdoviridae
SKIN MUCUS
biology.organism_classification
Virology
030104 developmental biology
chemistry
MONOCLONAL-ANTIBODIES
HOST-CELLS
Glycoprotein
Zoology
Developmental Biology
Subjects
Details
- ISSN :
- 0145305X
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- Developmental & Comparative Immunology
- Accession number :
- edsair.doi.dedup.....8f77933371b5fc46b685daffee4964d4