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244 results on '"Sagratini G"'

21. Synthesis and α1-adrenoceptor antagonist activity of tamsulosin analogues

Author

Sagratini G., Angeli P., Buccioni M., Gulini U., Marucci G., Poggesi E., Giardinà D., MELCHIORRE, CARLO, Sagratini G., Angeli P., Buccioni M., Gulini U., Marucci G., Melchiorre C., Poggesi E., and Giardinà D.

Abstract

Tamsulosin (-)-1 is the most utilized α(1)-adrenoceptor antagonist in the benign prostatic hyperplasia therapy owing to its uroselective antagonism and capability in relieving both obstructive and irritative lower urinary tract symptoms. Here we report the synthesis and pharmacological study of the homochiral (-)-1 analogues (-)-2-(-)-5, bearing definite modifications in the 2-substituted phenoxyethylamino group in order to evaluate their influence on the affinity profile for α(1)-adrenoceptor subtypes. The benzyl analogue (-)-3, displaying a preferential antagonist profile for α1A-than α1D-and α1B-adrenoceptors, and a 12-fold higher potency at α1A-adrenoceptors with respect to the α1B subtype, may have improved uroselectivity compared to (-)-1.

Published
2010

37. A preliminary matrix-assisted laser desorption/ionization time-of-flight approach for the characterization of Italian lentil varieties

Author

Caprioli, G., Cristalli, G., Ragazzi, E., Molin, L., Ricciutelli, M., Sagratini, G., Zuo, Y., and Vittori, S.

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used in this study to obtain protein fingerprints of seven different lentil varieties, to characterize their differences and similarities. Two different matrices have been tested in order to obtain reproducible and significant mass spectra. Extraction with water containing 0.1% of trifluoroacetic acid has been used as preparative step to obtain hydrophilic protein samples of lentil seeds. The obtained MALDI protein profiles identified clear differences between the seven studied lentil varieties. Moreover, considering the high complexity of the obtained MALDI spectra, multivariate techniques of data analysis were employed to find further classification details. These multivariate analyses confirmed the possibility of a clear classification of the seven lentil varieties, indicating that the proposed procedure can be a valid taxonomic tool, and a method to certify the origin of lentils, useful for high added value lentils (Italian lentils).

Published
2010

39. Quantification of Soyasaponins I and beta g in Italian Lentil Seeds by Solid-Phase Extraction (SPE) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)

Author

Sagratini, G., Zuo, Y.T., Caprioli, G., Cristalli, G., Giardina, D., Maggi, F., Molin, L., Ricciutelli, M., Traldi, P., and Vittori, S.

Abstract

Lentil saponins are triterpene glycosides, mainly soyasaponins I and beta g (also known as VI), with multiple health-promoting properties. This paper reports the isolation of soyasaponins I and beta g from soybeans as analytical standards and the development of a new analytical procedure for quantification of their content in various cultivars of Italian lentils, by SPE-HPLC-MS. Soyasaponins I and beta g were isolated from soybeans at a purity of >90% and characterized by MS/MS (ion trap) experiments. The determination of soyasaponins in lentils was performed by extraction, SPE purification, and HPLC-MS (single quadrupole) analysis; results were confirmed by MALDI-TOF experiments. Calibration curves for soyasaponin I and beta g showed correlation coefficients of 0.998 and 0.997, respectively. LOD and LOQ values were 0.02 and 0.2 mg kg(-1) for soyasaponins I and 0.1 and 1 mg kg(-1) for soyasaponin beta g. Recoveries calculated at a 100 mg kg(-1) fortification level ranged from 85 to 97%, with n = 10 and RSs of

Published
2009

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