34 results on '"Resca, E"'
Search Results
2. Ferutinin promotes proliferation and osteoblastic differentiation in human amniotic fluid and dental pulp stem cells
- Author
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Zavatti, M., Resca, E., Bertoni, L., Maraldi, T., Guida, M., Carnevale, G., Ferrari, A., and De Pol, A.
- Published
- 2013
- Full Text
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3. A new Approach to Investigate Biofilm Formation in Medical Devices
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Resca, E, Petrachi, T, Piccinno, Ms, Gavioli, G, Dominici, M, and Veronesi, E
- Published
- 2017
4. Enrichment in c-Kit improved differentiation potential of amniotic membrane progenitor/stem cells
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Resca, E., primary, Zavatti, M., additional, Maraldi, T., additional, Bertoni, L., additional, Beretti, F., additional, Guida, M., additional, La Sala, G.B., additional, Guillot, P.V., additional, David, A.L., additional, Sebire, N.J., additional, De Pol, A., additional, and De Coppi, P., additional
- Published
- 2015
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5. Multipotent stem cells in vitro differentiation on 3-D scaffolds
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Palumbo, Carla, Riccio, M., Resca, E., Maraldi, Tullia, Bertoni, Laura, Sena, Paola, and DE POL, Anto
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Multipotent stem cells. 3-D scaffolds. Osteogenic differentiation - Published
- 2009
6. Influence of ferutinin on bone mass and its side effects in ovariectomized rats
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Ferretti, Marzia, Palumbo, Carla, Bretoni, L., Cavani, Francesco, Resca, E., Benincasa, Marta, Carnevale, Gianluca, Zavatti, Manuela, Montanari, C., Benelli, A., Zanoli, P., and Marotti, Gastone
- Subjects
Ferutinin. Bone mass. Uterus. Ovariectomized (OVX) rats - Published
- 2008
7. Human dental pulp stem cells (HDPSC) versus osteoblast-like cells: comparison for capability of adhesion, growth and bone matrix formation on differently-shaped surfaces of biomaterials
- Author
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Palumbo, Carla, Riccio, M., Resca, E., Bretoni, L., Ferretti, Marzia, Cavani, Francesco, Bruzzesi, G., Baldini, Andrea, and DE POL, Anto
- Subjects
Human dental pulp stem cells (HDPSC). Osteoblast-like cells. Osteogenic differentiation. Biomaterials - Published
- 2008
8. Phytoestrogen effects on bone mass in ovariectomized rats: preliminary histomorphometric analysis
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Ferretti, Marzia, Palumbo, Carla, Cavani, Francesco, Bertoni, Laura, Resca, E., Carnevale, Gianluca, Zavatti, Manuela, Montanari, C., Benelli, A., Zanoli, P., and Marotti, Gastone
- Subjects
Phytoestrogen. Bone mass. Ovariectomized (OVX) rats - Published
- 2007
9. Placenta as a reservoir of stem cells: an underutilized resource?
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Pipino, C., primary, Shangaris, P., additional, Resca, E., additional, Zia, S., additional, Deprest, J., additional, Sebire, N. J., additional, David, A. L., additional, Guillot, P. V., additional, and De Coppi, P., additional
- Published
- 2012
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10. Role of NOX4 nuclear localization in Human Stem Cells
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Guida, M., primary, Riccio, M., additional, Resca, E., additional, De Pol, A., additional, and Maraldi⁎, T., additional
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- 2012
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11. Human dental pulp stem cells produce mineralized matrix in 2D and 3D cultures
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Riccio, M., primary, Resca, E., additional, Maraldi, T., additional, Pisciotta, A., additional, Ferrari, A., additional, Bruzzesi, G., additional, and De Pol, A., additional
- Published
- 2010
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12. Enrichment in c-Kit+ enhances mesodermal and neural differentiation of human chorionic placental cells.
- Author
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Resca, E., Zavatti, M., Bertoni, L., Maraldi, T., De Biasi, S., Pisciotta, A., Nicoli, A., La Sala, G.B., Guillot, P.V., David, A.L., Sebire, N.J., De Coppi, P., and De Pol, A.
- Abstract
Abstract: Objective: Human term placenta (HTP) has attracted increasing attention as an alternative source of stem cells for regenerative medicine since the amniochorionic membrane harbors stem cells populations that are easily accessible, abundantly available without ethical objections. In the chorionic side of HTP we found a progenitor perivascular “niche” in which rare cells co-express Oct-4 and c-Kit. We investigated the stem cell characteristics and differentiation potential of a chorionic derived population enriched in c-Kit
+ cells and compared this to the unenriched population. Study design: Cells, isolated from the chorion of HTP, were expanded and enriched in c-Kit+ cells (Chorionic Stem Cells-CSC). Histological staining, immunofluorescence, Western blot and flow cytometry were used to verify the stem cells characteristics of the populations and to compare the differentiation capability towards mesodermal and neural lineages in vitro. Results: The expression of the pluripotent marker Oct-4 was greater in the CSCs compared to the unselected cells (Chorionic Cell-CC) but both Oct-4 and c-Kit expression decreased during passages. After differentiation, CSC displayed stronger chondrogenic and osteogenic potential and a greater adipogenic forming capacity compared to unselected ones. CSC differentiated better into immature oligodendrocytes while CC showed a neuronal progenitor differentiation potential. Moreover, both populations were able to differentiate in hepatogenic lineage. Conclusion: CSC display improved Oct-4 expression and a high differentiation potential into mesodermal lineages and oligodendrocytes. [Copyright &y& Elsevier]- Published
- 2013
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13. Hybrid biofabricated blood vessel for medical devices testing.
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Portone A, Ganzerli F, Petrachi T, Resca E, Bergamini V, Accorsi L, Ferrari A, Sbardelatti S, Rovati L, Mari G, Dominici M, and Veronesi E
- Abstract
Current in vitro and in vivo tests applied to assess the safety of medical devices retain several limitations, such as an incomplete ability to faithfully recapitulate human features, and to predict the response of human tissues together with non-trivial ethical aspects. We here challenged a new hybrid biofabrication technique that combines bioprinting and Fast Diffusion-induced Gelation strategy to generate a vessel-like structure with the attempt to spatially organize fibroblasts, smooth-muscle cells, and endothelial cells. The introduction of Fast Diffusion-induced Gelation minimizes the endothelial cell mortality during biofabrication and produce a thin endothelial layer with tunable thickness. Cell viability, Von Willebrand factor, and CD31 expression were evaluated on biofabricated tissues, showing how bioprinting and Fast Diffusion-induced Gelation can replicate human vessels architecture and complexity. We then applied biofabricated tissue to study the cytotoxicity of a carbothane catheter under static condition, and to better recapitulate the effect of blood flow, a novel bioreactor named CuBiBox (Customized Biological Box) was developed and introduced in a dynamic modality. Collectively, we propose a novel bioprinted platform for human in vitro biocompatibility testing, predicting the impact of medical devices and their materials on vascular systems, reducing animal experimentation and, ultimately, accelerating time to market., Competing Interests: No potential conflict of interest was reported by the author(s)., (© 2024 The Author(s). Published by National Institute for Materials Science in partnership with Taylor & Francis Group.)
- Published
- 2024
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14. Novel bioprinted 3D model to human fibrosis investigation.
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Petrachi T, Portone A, Arnaud GF, Ganzerli F, Bergamini V, Resca E, Accorsi L, Ferrari A, Delnevo A, Rovati L, Marra C, Chiavelli C, Dominici M, and Veronesi E
- Subjects
- Animals, Humans, Fibrosis, Cell Differentiation physiology, Extracellular Matrix metabolism, Fibroblasts metabolism, Collagen Type I metabolism, Transforming Growth Factor beta metabolism
- Abstract
Fibrosis is shared in multiple diseases with progressive tissue stiffening, organ failure and limited therapeutic options. This unmet need is also due to the lack of adequate pre-clinical models to mimic fibrosis and to be challenged novel by anti-fibrotic therapeutic venues. Here using bioprinting, we designed a novel 3D model where normal human healthy fibroblasts have been encapsulated in type I collagen. After stimulation by Transforming Growth factor beta (TGFβ), embedded cells differentiated into myofibroblasts and enhanced the contractile activity, as confirmed by the high level of α - smooth muscle actin (αSMA) and F-actin expression. As functional assays, SEM analysis revealed that after TGFβ stimulus the 3D microarchitecture of the scaffold was dramatically remolded with an increased fibronectin deposition with an abnormal collagen fibrillar pattern. Picrius Sirius Red staining additionally revealed that TGFβ stimulation enhanced of two logarithm the collagen fibrils neoformation in comparison with control. These data indicate that by bioprinting technology, it is possible to generate a reproducible and functional 3D platform to mimic fibrosis as key tool for drug discovery and impacting on animal experimentation and reducing costs and time in addressing fibrosis., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Tiziana Petrachi, Alberto Portone, Gaelle Francoise Arnaud, Francesco Ganzerli, Valentina Bergamini, Elisa Resca, Luca Accorsi, Alberto Ferrari, Annalisa Delnevo, Luigi Rovati, Caterina Marra, Chiara Chiavelli, Massimo Dominici and Elena Veronesi DECLARE NO competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Published
- 2023
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15. Label-Free Optical Sensing and Medical Grade Resins: An Advanced Approach to Investigate Cell-Material Interaction and Biocompatibility.
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Bergamini V, Resca E, Portone A, Petrachi T, Ganzerli F, Truzzi S, Mari G, Rovati L, Dominici M, and Veronesi E
- Abstract
The Corning Epic
® label-free (ELF) system is an innovative technology widely used in drug discovery, immunotherapy, G-protein-associated studies, and biocompatibility tests. Here, we challenge the use of ELF to further investigate the biocompatibility of resins used in manufacturing of blood filters, a category of medical devices representing life-saving therapies for the increasing number of patients with kidney failure. The biocompatibility assays were carried out by developing a cell model aimed at mimicking the clinical use of the blood filters and complementing the existing cytotoxicity assay requested by ISO10993-5. Experiments were performed by putting fibroblasts in both direct contact with two types of selected resins, and indirect contact by means of homemade customized well inserts that were precisely designed and developed for this technology. For both types of contact, fibroblasts were cultured in medium and human plasma. ELF tests confirmed the biocompatibility of both resins, highlighting a statistically significant different biological behavior of a polyaromatic resin compared to control and ion-exchanged resin, when materials were in indirect contact and soaking with plasma. Overall, the ELF test is able to mimic clinical scenarios and represents a promising approach to investigate biocompatibility, showing peculiar biological behaviors and suggesting the activation of specific intracellular pathways.- Published
- 2023
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16. A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings.
- Author
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Gallingani T, Resca E, Dominici M, Gavioli G, Laurita R, Liguori A, Mari G, Ortolani L, Pericolini E, Sala A, Laghi G, Petrachi T, Arnauld GF, Accorsi L, Rizzoli R, Colombo V, Gherardi M, and Veronesi E
- Subjects
- Mice, Animals, Coated Materials, Biocompatible chemistry, Anti-Bacterial Agents pharmacology, Biofilms, Silver chemistry, Metal Nanoparticles
- Abstract
In industrialized countries, health care associated infections, the fourth leading cause of disease, are a major health issue. At least half of all cases of nosocomial infections are associated with medical devices. Antibacterial coatings arise as an important approach to restrict the nosocomial infection rate without side effects and the development of antibiotic resistance. Beside nosocomial infections, clot formation affects cardiovascular medical devices and central venous catheters implants. In order to reduce and prevent such infection, we develop a plasma-assisted process for the deposition of nanostructured functional coatings on flat substrates and mini catheters. Silver nanoparticles (Ag NPs) are synthesized exploiting in-flight plasma-droplet reactions and are embedded in an organic coating deposited through hexamethyldisiloxane (HMDSO) plasma assisted polymerization. Coating stability upon liquid immersion and ethylene oxide (EtO) sterilization is assessed through chemical and morphological analysis carried out by means of Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). In the perspective of future clinical application, an in vitro analysis of anti-biofilm effect has been done. Moreover, we employed a murine model of catheter-associated infection which further highlighted the performance of Ag nanostructured films in counteract biofilm formation. The anti-clot performances coupled by haemo- and cytocompatibility assays have also been performed., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Gallingani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2023
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17. Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance.
- Author
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Piccinno MS, Petrachi T, Pignatti M, Murgia A, Grisendi G, Candini O, Resca E, Bergamini V, Ganzerli F, Portone A, Mastrolia I, Chiavelli C, Castelli I, Bernabei D, Tagliazucchi M, Bonetti E, Lolli F, De Santis G, Dominici M, and Veronesi E
- Subjects
- Animals, Green Fluorescent Proteins metabolism, Humans, Mice, Mice, Inbred NOD, Mice, SCID, Rabbits, Adipose Tissue, Mesenchymal Stem Cells metabolism
- Abstract
The resorption rate of autologous fat transfer (AFT) is 40-60% of the implanted tissue, requiring new surgical strategies for tissue reconstruction. We previously demonstrated in a rabbit model that AFT may be empowered by adipose-derived mesenchymal stromal/stem cells (AD-MSCs), which improve graft persistence by exerting proangiogenic/anti-inflammatory effects. However, their fate after implantation requires more investigation. We report a xenograft model of adipose tissue engineering in which NOD/SCID mice underwent AFT with/without human autologous AD-MSCs and were monitored for 180 days (d). The effect of AD-MSCs on AFT grafting was also monitored by evaluating the expression of CD31 and F4/80 markers. Green fluorescent protein-positive AD-MSCs (AD-MSC-GFP) were detected in fibroblastoid cells 7 days after transplantation and in mature adipocytes at 60 days, indicating both persistence and differentiation of the implanted cells. This evidence also correlated with the persistence of a higher graft weight in AFT-AD-MSC compared to AFT alone treated mice. An observation up to 180 d revealed a lower resorption rate and reduced lipidic cyst formation in the AFT-AD-MSC group, suggesting a long-term action of AD-MSCs in support of AFT performance and an anti-inflammatory/proangiogenic activity. Together, these data indicate the protective role of adipose progenitors in autologous AFT tissue resorption., Competing Interests: The authors declare no conflict of interest.
- Published
- 2022
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18. Microfragmented adipose tissue is associated with improved ex vivo performance linked to HOXB7 and b-FGF expression.
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Casari G, Resca E, Giorgini A, Candini O, Petrachi T, Piccinno MS, Foppiani EM, Pacchioni L, Starnoni M, Pinelli M, De Santis G, Selleri F, Catani F, Dominici M, and Veronesi E
- Subjects
- Adipose Tissue, Cell Differentiation, Cells, Cultured, Synovial Fluid, Genes, Homeobox, Mesenchymal Stem Cells
- Abstract
Introduction: Adipose tissue (AT) has become a source of mesenchymal stromal/stem cells (MSC) for regenerative medicine applications, in particular skeletal disorders. Several enzymatic or mechanical procedures have been proposed to process AT with the aim to isolate cells that can be locally implanted. How AT is processed may impact its properties. Thus, we compared AT processed by centrifugation (C-AT) to microfragmentation (MF-AT). Focusing on MF-AT, we subsequently assessed the impact of synovial fluid (SF) alone on both MF-AT and isolated AT-MSC to better understand their cartilage repair mechanisms., Materials and Methods: MF-AT and C-AT from the same donors were compared by histology and qRT-PCR immediately after isolation or as ex vivo cultures using a micro-tissue pellet system. The in vitro impact of SF on MF-AT and AT-MSC was assessed by histological staining and molecular analysis., Results: The main AT histological features (i.e., increased extracellular matrix and cellularity) of the freshly isolated or ex vivo-cultured MF-AT persisted compared to C-AT, which rapidly deteriorated during culture. Based on our previous studies of HOX genes in MSC, we investigated the involvement of Homeobox Protein HOX-B7 (HOXB7) and its target basic Fibroblast Growth Factor (bFGF) in the molecular mechanism underlying the improved performance of MF-AT. Indeed, both these biomarkers were more prominent in freshly isolated MF-AT compared to C-AT. SF alone preserved the AT histological features of MF-AT, together with HOXB7 and bFGF expression. Increased cell performance was also observed in isolated AT-MSC after SF treatment concomitant with enhanced HOXB7 expression, although there was no apparent association with bFGF., Conclusions: Our findings show that MF has a positive effect on the maintenance of AT histology and may trigger the expression of trophic factors that improve tissue repair by processed AT., (© 2021. The Author(s).)
- Published
- 2021
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19. Assessing Biocompatibility of Face Mask Materials during COVID-19 Pandemic by a Rapid Multi-Assays Strategy.
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Petrachi T, Ganzerli F, Cuoghi A, Ferrari A, Resca E, Bergamini V, Accorsi L, Burini F, Pasini D, Arnaud GF, Piccini M, Aldrovandi L, Mari G, Tomasi A, Rovati L, Dominici M, and Veronesi E
- Subjects
- Humans, Masks, SARS-CoV-2, Textiles, COVID-19, Pandemics
- Abstract
During the coronavirus disease 2019 (COVID-19) pandemic, scientific authorities strongly suggested the use of face masks (FMs). FM materials (FMMs) have to satisfy the medical device biocompatibility requirements as indicated in the technical standard EN ISO 10993-1:2018. The biologic evaluation must be confirmed by in vivo tests to verify cytotoxicity, sensitisation, and skin irritation. Some of these tests require an extensive period of time for their execution, which is incompatible with an emergency situation. In this study, we propose to verify the safety of FMMs combining the assessment of 3-[4,5-dimethylthiazolyl-2]-2,5-diphenyltetrazolium bromide (MTT) with quantification of nitric oxide (NO) and interleukin-6 (IL-6), as predictive markers of skin sensitisation or irritation based on human primary fibroblasts. Two hundred and forty-two FMMs were collected and classified according to spectrometer IR in polypropylene, paper, cotton, polyester, polyethylene terephthalate, 3-dimensional printing, and viscose. Of all FMMs tested, 50.8% passed all the assays, 48% failed at least one, and only 1.2% failed all. By a low cost, rapid and highly sensitive multi assays strategy tested on human skin fibroblasts against a large variety of FMMs, we propose a strategy to promptly evaluate biocompatibility in wearable materials.
- Published
- 2021
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20. Microscopic and chemical characterization of PVC tube used for dialysis lines: A new approach.
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Petrachi T, Arnaud GF, Roncioni S, Resca E, Veronesi E, Dominici M, Tomasi A, and Cuoghi A
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- Humans, Materials Testing methods, Plastics chemistry, Plastics therapeutic use, Platelet Aggregation, Equipment Safety methods, Polyvinyl Chloride adverse effects, Polyvinyl Chloride chemistry, Polyvinyl Chloride therapeutic use, Renal Dialysis instrumentation, Spectroscopy, Fourier Transform Infrared methods, Surface Properties
- Abstract
Polyvinylchloride is universally agreed upon to be the material of choice for tubings and for containers for medical application. Many alterations of the chemical/physical surface conditions, mainly due to an altered extrusion process, could influence its biocompatibility by promoting platelet aggregation. Biocompatibility and safety of the medical device must be preserved, also monitoring the migration of additives within polyvinylchloride during the diffusion process. A large variety of methods are used to verify the correct composition and extrusion of polyvinylchloride but, generally, they need long experimental time and are expensive. The aim of the study is to propose a simple, economic and rapid approach based on Fourier transform-infrared spectroscopy and Coomassie Blue staining. The method has been used to detect chemical and morphological defects caused by an altered extrusion process on 20/75 polyvinylchloride tubings in a blind test. This approach positively identified altered samples in 80% of the cases. The suggested approach represents a reliable and versatile method to detect and monitor surface defects by an easy, inexpensive and reproducible method.
- Published
- 2021
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21. Label-free toxicology screening of primary human mesenchymal cells and iPS-derived neurons.
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Piccinno MS, Petrachi T, Resca E, Strusi V, Bergamini V, Mulas GA, Mari G, Dominici M, and Veronesi E
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- Drug Evaluation, Preclinical instrumentation, Drug Evaluation, Preclinical methods, Humans, Induced Pluripotent Stem Cells pathology, Mesenchymal Stem Cells pathology, Neurons pathology, Primary Cell Culture, Cell Differentiation, Induced Pluripotent Stem Cells metabolism, Mesenchymal Stem Cells metabolism, Methylmethacrylate toxicity, Neurons metabolism
- Abstract
The high-throughput, label-free Corning Epic assay has applications in drug discovery, pharmacogenomics, cell receptor signaling, cell migration, and viral titration. The utility of Epic technology for biocompatibility testing has not been well established. In manufacturing of medical devices, in vitro and in vivo biocompatibility assessments are mandatory, according to ISO 10993. The new medical device regulation MDR 745/2017 specifies that ex vivo assays that can closely recapitulate in vivo scenarios are needed to better evaluate biomedical devices. We propose herein that Epic technology-which enables detection of variations in cell mass distribution-is suitable for biocompatibility screening of compounds. In this study, we challenged primary human osteoblasts, endothelial cells, and neurons derived from induced pluripotent stem cells with specific concentrations of methyl methacrylate (MMA). Polymeric MMA has long been applied in cranioplasty, where it makes contact with multiple cell types. Application of Epic technology yielded real-time cytotoxicity profiles for all considered cell types. The results were compared with those from microscopic observation of the same culture plate used in the Epic analyses. The Epic assay should be further examined for its utility for cell biology, genomics, and proteomics companion assays. Our results suggest that Epic technology can be applied to biocompatibility evaluation of human cells in medical device development., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2018
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22. An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study.
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Petrachi T, Resca E, Piccinno MS, Biagi F, Strusi V, Dominici M, and Veronesi E
- Subjects
- Feasibility Studies, Gentian Violet chemistry, Bacteriological Techniques methods, Biofilms growth & development, Equipment and Supplies microbiology, Microscopy methods
- Abstract
Biofilms are assemblages of bacterial cells irreversibly associated with a surface where moisture is present. In particular, they retain a relevant impact on public health since through biofilms bacteria are able to survive and populate biomedical devices causing severe nosocomial infections that are generally resistant to antimicrobial agents. Therefore, controlling biofilm formation is a mandatory feature during medical device manufacturing and during their use. In this study, combining a crystal violet staining together with advanced stereomicroscopy, we report an alternative rapid protocol for both qualitative and semi-quantitative biofilm determination having high specificity, high repeatability, and low variability. The suggested approach represents a reliable and versatile method to detect, monitor, and measure biofilm colonization by an easy, more affordable, and reproducible method., Competing Interests: The authors declare no conflict of interest.
- Published
- 2017
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23. Human biliary tree stem/progenitor cells immunomodulation: Role of hepatocyte growth factor.
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Maraldi T, Guida M, Beretti F, Resca E, Carpino G, Cardinale V, Gentile R, Ardizzoni A, Murgia A, Alvaro D, Gaudio E, and De Pol A
- Abstract
Aim: Human biliary tree stem/progenitor cells (hBTSC) are multipotent epithelial stem cells with the potential for allogenic transplant in liver, biliary tree, and pancreatic diseases. Human mesenchymal stem cells, but also epithelial stem cells, are able to modulate immune responses with different types of secretion molecules., Methods: The initial aim of the present study was to develop for the first time a culture protocol in order to expand hBTSC in vitro through passages, allowing to maintain a similar stem cell and secretome profile. Furthermore, we investigated the secretome profile of the hBTSC to assess the production of molecules capable of affecting immune feedback., Results: We found that hepatocyte growth factor produced by hBTSC exerts its cytoprotective role inducing apoptosis in human immune cells, such as lymphocytes., Conclusions: The present study, therefore, supports the hypothesis that hBTSC can be useful for the purpose of regenerative medicine, as they can be banked and expanded, and they can secrete immunoregulatory factors., (© 2016 The Japan Society of Hepatology.)
- Published
- 2017
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24. NADPH oxidase-4 and MATER expressions in granulosa cells: Relationships with ovarian aging.
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Maraldi T, Resca E, Nicoli A, Beretti F, Zavatti M, Capodanno F, Morini D, Palomba S, La Sala GB, and De Pol A
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- Female, Granulosa Cells enzymology, Humans, Mitochondrial Proteins, NADPH Oxidase 4, Nuclear Proteins, Autoantigens metabolism, Cellular Senescence, Granulosa Cells metabolism, NADPH Oxidases metabolism, Ovary physiology
- Abstract
Aims: Relevant roles in follicular development and ovulation are played by maternal antigen that embryos require (MATER), product of a maternal effect gene, and by reactive oxygen species (ROS), indispensable for the induction of ovulatory genes. At the moment, the relationship between these two biological systems and their involvement in the ovarian aging have not been still clarified. The aim of the current experimental study was to analyse the age-related changes of the MATER and NOX proteins., Materials and Methods: MATER and ROS homeostasis was studied in granulosa cells (GCs) and cumulus cells (CCs) of infertile patients who undergone oocyte retrieval for in vitro fertilization cycles using Western blot and confocal immunofluorescence analysis. Samples were obtained from subjects with age≥40years (cases) and with age≤37years (controls)., Key Findings: The expression pattern of MATER and NOX observed in GCs was not different from that observed in CCs. High levels of both proteins were detected in the control samples. A significant lower expression of both MATER and NOX4 was observed in the case versus control samples., Significance: The expression of MATER and NOX4 proteins are closely related to the follicular development and ovulation with particular regard for ovarian aging., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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25. Critical-size bone defect repair using amniotic fluid stem cell/collagen constructs: effect of oral ferutinin treatment in rats.
- Author
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Zavatti M, Bertoni L, Maraldi T, Resca E, Beretti F, Guida M, La Sala GB, and De Pol A
- Subjects
- Animals, Bridged Bicyclo Compounds pharmacology, Cell Differentiation drug effects, Cell Survival drug effects, Estrogen Receptor alpha metabolism, Humans, Male, Osteocalcin metabolism, Rats, Receptors, G-Protein-Coupled metabolism, Amniotic Fluid cytology, Benzoates pharmacology, Bone Development drug effects, Collagen pharmacology, Cycloheptanes pharmacology, Sesquiterpenes pharmacology, Stem Cell Transplantation
- Abstract
Aims: This study aims to evaluate the bone regeneration in a rat calvarias critical size bone defect treated with a construct consisting of collagen type I and human amniotic fluid stem cells (AFSCs) after oral administration of phytoestrogen ferutinin., Main Methods: In 12 week old male rats (n=10), we performed two symmetric full-thickness cranial defects on each parietal region, and a scaffold was implanted into each cranial defect. The rats were divided into four groups: 1) collagen scaffold, 2) collagen scaffold+ferutinin at a dose of 2mg/kg/5 mL, 3) collagen scaffold + AFSCs, and 4) collagen scaffold + AFSCs + ferutinin. The rats were sacrificed after 4 weeks, and the calvariae were removed, fixed, embedded in paraffin and cut into 7 μm thick sections. Histomorphometric measures, immunohistochemical and immunofluorescence analyses were performed on the paraffin sections., Key Findings: The histomorphometric analysis on H&E stained sections showed a significant increase in the regenerated area of the 4th group compared with the other groups. Immunohistochemistry performed with a human anti-mitochondrial antibody showed the presence of AFSCs 4 weeks after the transplant. Immunofluorescence analysis revealed the presence of osteocalcin and estrogen receptors (ERα and GPR30) in all groups, with a greater expression of all markers in samples where the scaffold was treated with AFSCs and the rats were orally administered ferutinin., Significance: Our results demonstrated that the oral administration of ferutinin is able to improve the bone regeneration of critical-size bone defects in vivo that is obtained with collagen-AFSCs constructs., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2015
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26. Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells.
- Author
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Maraldi T, Guida M, Zavatti M, Resca E, Bertoni L, La Sala GB, and De Pol A
- Subjects
- Cell Differentiation, Cell Proliferation, Cells, Cultured, Humans, NADPH Oxidase 4, Amniotic Fluid immunology, NADPH Oxidases genetics, NADPH Oxidases metabolism, Stem Cells metabolism
- Abstract
Human amniotic fluid stem cells (AFSC) are an attractive source for cell therapy due to their multilineage differentiation potential and accessibility advantages. However the clinical application of human stem cells largely depends on their capacity to expand in vitro, since there is an extensive donor-to-donor heterogeneity. Reactive oxygen species (ROS) and cellular oxidative stress are involved in many physiological and pathophysiological processes of stem cells, including pluripotency, proliferation, differentiation, and stress resistance. The mode of action of ROS is also dependent on the localization of their target molecules. Thus, the modifications induced by ROS can be separated depending on the cellular compartments they affect. NAD(P)H oxidase family, particularly Nox4, has been known to produce ROS in the nucleus. In the present study we show that Nox4 nuclear expression (nNox4) depends on the donor and it correlates with the expression of transcription factors involved in stemness regulation, such as Oct4, SSEA-4, and Sox2. Moreover nNox4 is linked with the nuclear localization of redox sensitive transcription factors, as Nrf2 and NF-κB, and with the differentiation potential. Taken together, these results suggest that nNox4 regulation may have important effects in stem cell capability through modulation of transcription factors and DNA damage.
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- 2015
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27. Human amniotic fluid stem cells: neural differentiation in vitro and in vivo.
- Author
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Maraldi T, Bertoni L, Riccio M, Zavatti M, Carnevale G, Resca E, Guida M, Beretti F, La Sala GB, and De Pol A
- Subjects
- Amniotic Fluid metabolism, Animals, Cell Differentiation physiology, Humans, In Vitro Techniques, Neurons metabolism, Rats, Regenerative Medicine, Stem Cells metabolism, Amniotic Fluid cytology, Neurons cytology, Stem Cells cytology
- Abstract
The successful integration of stem cells after their implantation into the brain has become a central issue in modern neuroscience. In this study, we test the neural differentiation potential of c-Kit(+)/Oct-4(+) human amniotic fluid stem cells (hAFSCs) in vitro and their survival and integration in vivo. hAFSCs were induced towards neural differentiation and specific markers (GFAP, β-III tubulin, CNPase, MAP2, NeuN, synapsines, S100, PMP22) were detected by immunofluorescence and Western blot analysis. Glial proteins were expressed as early as 2 weeks after the initial differentiation stimulus, whereas neuronal markers started to appear from the third week of differentiation under culturing conditions of high cell density. This timeline suggested that glial cells possessed a promoting role in the differentiation of hAFSCs towards a neuronal fate. hAFSCs were then implanted into the lateral ventricle of the brain of 1-day-old rats, since neuronal development occurs up to 1 month after birth in this animal model. Our data showed that hAFSCs survived for up to 6 weeks post-implantation, were integrated into various areas of the central nervous system and migrated away from the graft giving rise to mature neurons and oligodendrocytes. We conclude that hAFSCs are able to differentiate and integrate into nervous tissue during development in vivo.
- Published
- 2014
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28. Inhibition of nuclear Nox4 activity by plumbagin: effect on proliferative capacity in human amniotic stem cells.
- Author
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Guida M, Maraldi T, Resca E, Beretti F, Zavatti M, Bertoni L, La Sala GB, and De Pol A
- Subjects
- Adult, Cell Proliferation drug effects, DNA Damage, Female, Fluorescent Antibody Technique, Humans, NADPH Oxidase 4, NADPH Oxidases metabolism, Protein Transport drug effects, Reactive Oxygen Species metabolism, Serum metabolism, Stem Cells drug effects, Subcellular Fractions drug effects, Subcellular Fractions metabolism, Amniotic Fluid cytology, Cell Nucleus enzymology, NADPH Oxidases antagonists & inhibitors, Naphthoquinones pharmacology, Stem Cells cytology, Stem Cells enzymology
- Abstract
Human amniotic fluid stem cells (AFSC) with multilineage differentiation potential are novel source for cell therapy. However, in vitro expansion leads to senescence affecting differentiation and proliferative capacities. Reactive oxygen species (ROS) have been involved in the regulation of stem cell pluripotency, proliferation, and differentiation. Redox-regulated signal transduction is coordinated by spatially controlled production of ROS within subcellular compartments. NAD(P)H oxidase family, in particular Nox4, has been known to produce ROS in the nucleus; however, the mechanisms and the meaning of this function remain largely unknown. In the present study, we show that Nox4 nuclear expression (nNox4) increases during culture passages up to cell cycle arrest and the serum starvation causes the same effect. With the decrease of Nox4 activity, obtained with plumbagin, a decline of nuclear ROS production and of DNA damage occurs. Moreover, plumbagin exposure reduces the binding between nNox4 and nucleoskeleton components, as Matrin 3. The same effect was observed also for the binding with phospho-ERK, although nuclear ERK and P-ERK are unchanged. Taken together, we suggest that nNox4 regulation may have important pathophysiologic effects in stem cell proliferation through modulation of nuclear signaling and DNA damage.
- Published
- 2013
- Full Text
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29. Placenta as a reservoir of stem cells: an underutilized resource?
- Author
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Pipino C, Shangaris P, Resca E, Zia S, Deprest J, Sebire NJ, David AL, Guillot PV, and De Coppi P
- Subjects
- Adult, Animals, Cell Differentiation, Epithelial Cells cytology, Epithelial Cells metabolism, Female, Humans, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Mice, Placenta cytology, Pluripotent Stem Cells cytology, Pluripotent Stem Cells metabolism, Pregnancy, Rats, Stem Cells metabolism, Health Resources statistics & numerical data, Placenta metabolism, Stem Cells cytology
- Abstract
Introduction: Both embryonic and adult tissues are sources of stem cells with therapeutic potential but with some limitations in the clinical practice such as ethical considerations, difficulty in obtaining and tumorigenicity. As an alternative, the placenta is a foetal tissue that can be obtained during gestation and at term, and it represents a reservoir of stem cells with various potential., Sources of Data: We reviewed the relevant literature concerning the main stem cells that populate the placenta., Areas of Agreement: Recently, the placenta has become useful source of stem cells that offer advantages in terms of proliferation and plasticity when compared with adult cells and permit to overcome the ethical and safety concern inherent in embryonic stem cells. In addition, the placenta has the advantage of containing epithelia, haematopoietic and mesenchymal stem cells. These stem cells possess immunosuppressive properties and have the capacity of suppress in vivo inflammatory responses., Areas of Controversy: Some studies describe a subpopulation of placenta stem cells expressing pluripotency markers, but for other studies, it is not clear whether pluripotent stem cells are present during gestation beyond the first few weeks. Particularly, the expression of some pluripotency markers such as SSEA-3, TRA-1-60 and TRA-1-81 has been reported by us, but not by others., Growing Points: Placenta stem cells could be of great importance after delivery for banking for autologous and allogeneic applications. The beneficial effects of these cells may be due to secretion of bioactive molecules that act through paracrine actions promoting beneficial effects., Areas Timely for Developing Research: Understanding the role of placenta stem cells during pregnancy and their paracrine actions could help in the study of some diseases that affect the placenta during pregnancy.
- Published
- 2013
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30. Human amniotic fluid stem cells seeded in fibroin scaffold produce in vivo mineralized matrix.
- Author
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Maraldi T, Riccio M, Resca E, Pisciotta A, La Sala GB, Ferrari A, Bruzzesi G, Motta A, Migliaresi C, Marzona L, and De Pol A
- Subjects
- Animals, Blotting, Western, Bombyx, Cell Differentiation physiology, Cells, Cultured, Collagen chemistry, Core Binding Factor Alpha 1 Subunit metabolism, Fluorescent Antibody Technique, Humans, Microscopy, Atomic Force, Microscopy, Confocal, Osteocalcin metabolism, Osteogenesis physiology, Osteopontin metabolism, Sp7 Transcription Factor, Transcription Factors metabolism, Amniotic Fluid cytology, Fibroins chemistry, Silk chemistry, Stem Cells cytology, Tissue Scaffolds chemistry
- Abstract
This study investigated the potential of amniotic fluid stem cells (AFSCs) to synthesize mineralized extracellular matrix (ECM) within different porous scaffolds of collagen, poly-D,L-lactic acid (PDLLA), and silk fibroin. The AFSCs were initially differentiated by using an osteogenic medium in two-dimensional culture, and expression of specific bone proteins and the physiologic mineral production by the AFSCs were analyzed. In particular, during differentiation process, AFSCs expressed proteins like Runt-related transcription factor 2 (Runx2), Osterix, Osteopontin, and Osteocalcin with a sequential expression, analogous to those occurring during osteoblast differentiation, and produced extracellular calcium stores. AFSCs were then cultured on three-dimensional (3D) scaffolds and evaluated for their ability to differentiate into osteoblastic cells in vivo. Stem cells were cultured in vitro for 1 week in collagen, fibroin, and PDLLA scaffolds. The effect of predifferentiation of the stem cells in scaffolds on the subsequent bone formation in vivo was determined in a rat subcutaneous model. With the addition of a third dimension, osteogenic differentiation and mineralized ECM production by AFSCs were significantly higher. This study demonstrated the strong potential of AFSCs to produce 3D mineralized bioengineered constructs in vivo and suggests that fibroin may be an effective scaffold material for functional repair of critical size bone defects.
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- 2011
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31. Brain lipid-binding protein: a marker of differentiation in neuroblastic tumors.
- Author
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Retrosi G, Sebire NJ, Bishay M, Kiely EM, Anderson J, De Coppi P, Resca E, Rampling D, Bier N, Mills K, Eaton S, and Pierro A
- Subjects
- Biopsy, Needle, Brain Neoplasms diagnosis, Child, Child, Preschool, Diagnosis, Differential, Fatty Acid-Binding Protein 7, Female, Ganglioneuroblastoma diagnosis, Ganglioneuroblastoma pathology, Ganglioneuroma diagnosis, Humans, Immunohistochemistry, Male, Neuroblastoma diagnosis, Sensitivity and Specificity, Biomarkers, Tumor metabolism, Brain Neoplasms pathology, Carrier Proteins metabolism, Ganglioneuroma pathology, Neuroblastoma pathology, Tumor Suppressor Proteins metabolism
- Abstract
Purpose: Neuroblastoma (NB), ganglioneuroblastoma (GNB), and ganglioneuroma (GN) are neuroblastic tumours (NT) of sympathetic nervous system origin. Brain lipid-binding protein (BLBP) has potential morphogenic activity during nervous system development but has not been studied in these tumours. We analyzed the expression of BLBP in NT according to histological subtypes and extent of differentiation., Methods: Thirty cases of NT (10 each of NB, intermixed GNB, and GN) were identified from the histopathology archive of a single center. Tissue sections were obtained from representative paraffin blocks and immunohistochemistry for BLBP performed., Results: Brain lipid-binding protein was not expressed in any NB case. In all cases of GN, BLBP was strongly expressed in the cytoplasm of mature ganglion cells but negative in Schwannian stroma. In the intermixed GNB, there was similar strong BLBP immunoreactivity in the cytoplasm of fully differentiated and differentiating ganglion cells but no BLBP expression in immature neuroblasts., Conclusion: Brain lipid-binding protein is strongly expressed in mature and maturing ganglion cells in NT (GN and GNB), whereas it is absent in poorly differentiated neuroblasts of GNB and NB. Cytoplasmic expression of BLBP in NT increases as the cells undergo neural differentiation and is therefore associated with the extent of tumour differentiation and favorable histology., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2011
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32. Influence of ferutinin on bone metabolism in ovariectomized rats. II: role in recovering osteoporosis.
- Author
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Ferretti M, Bertoni L, Cavani F, Zavatti M, Resca E, Carnevale G, Benelli A, Zanoli P, and Palumbo C
- Subjects
- Animals, Benzoates pharmacology, Body Weight drug effects, Bone Density Conservation Agents pharmacology, Bone and Bones metabolism, Bone and Bones pathology, Bridged Bicyclo Compounds pharmacology, Bridged Bicyclo Compounds therapeutic use, Calcium blood, Cycloheptanes pharmacology, Disease Models, Animal, Drug Evaluation, Preclinical, Estrogens deficiency, Female, Femur drug effects, Femur metabolism, Femur pathology, Lumbar Vertebrae drug effects, Lumbar Vertebrae metabolism, Lumbar Vertebrae pathology, Magnesium blood, Osteoporosis pathology, Osteoporosis physiopathology, Ovariectomy, Phosphorus blood, Rats, Rats, Sprague-Dawley, Sesquiterpenes pharmacology, Benzoates therapeutic use, Bone Density Conservation Agents therapeutic use, Bone and Bones drug effects, Cycloheptanes therapeutic use, Osteoporosis drug therapy, Sesquiterpenes therapeutic use
- Abstract
The aim of the present investigation, which represents an extension of a previous study, was to investigate the effect of ferutinin in recovering severe osteoporosis due to estrogen deficiency after rat ovariectomy and to compare phytoestrogen effects with those of estrogens commonly used in hormone replacement therapy (HRT) by women with postmenopausal osteoporosis. The animal model used was the Sprague-Dawley ovariectomized rat. Ferutinin was orally administered (2 mg kg(-1) per day) for 30 or 60 days starting from 2 months after ovariectomy (i.e. when osteoporosis was clearly evident) and its effects were compared with those of estradiol benzoate (1.5 microg per rat twice a week, subcutaneously injected) vs. vehicle-treated ovariectomized (OVX) and sham-operated (SHAM) rats. Histomorphometric analyses were performed on trabecular bone of lumbar vertebrae (4th and 5th) and distal femoral epiphysis, as well as on cortical bone of femoral diaphysis. Bone histomorphometric analyses showed that ferutinin seems to display the same effects on bone mass recorded with estradiol benzoate, thus suggesting that it could enhance the recovery of bone loss due to severe estrogen deficiency in OVX rats. On this basis, the authors propose listing ferutinin among the substances representing a potential alternative for the treatment of postmenopausal osteoporosis, which occurs as a result of estrogen deficiency.
- Published
- 2010
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33. Leptin increases growth of primary ossification centers in fetal mice.
- Author
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Bertoni L, Ferretti M, Cavani F, Zavatti M, Resca E, Benelli A, and Palumbo C
- Subjects
- Animals, Bone and Bones drug effects, Cell Differentiation drug effects, Cell Proliferation drug effects, Female, Maternal-Fetal Exchange physiology, Mice, Organogenesis drug effects, Osteogenesis physiology, Pregnancy, Prenatal Exposure Delayed Effects, Bone and Bones embryology, Leptin pharmacology, Osteogenesis drug effects
- Abstract
The effect of peripheral leptin on fetal primary ossification centers during the early phases of bone histogenesis was investigated by administration of leptin to pregnant mice. Fourteen pregnant mice were divided into two groups. The treated pregnant group was subcutaneously injected in the intrascapular region with supraphysiologic doses (2 mg kg(-1)) of leptin (Vinci Biochem, Firenze, Italy) in a volume of 0.1 mL per 10 g body weight, at the 7th, 9th and 11th day of gestation. The control group was treated with physiological solution in the same manner and same times as the treated group. The new-born mice were killed 1 day after birth and the primary ossification centers were stained with Alizarin Red S after diaphanizing the soft tissues in 1% potassium hydroxide. The development of both endochondral and intramembranous ossification centers was morphometrically analysed in long bones. The results showed that the ossification centers of mice born by mothers treated with leptin grow more rapidly in both length and cross-sectional area compared with mice born by the untreated mothers. As the development of long bones depends on endochondral ossification occurring at proximal and distal epiphyseal plates as well as on intramembranous ossification along the periosteal surface, it appears that leptin activates the differentiation and proliferation of both chondrocytes and osteoblasts. The role of leptin as a growth factor of cartilage and bone is discussed in the light of the data reported in the literature.
- Published
- 2009
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- View/download PDF
34. Influence of ferutinin on bone metabolism in ovariectomized rats. I: role in preventing osteoporosis.
- Author
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Palumbo C, Ferretti M, Bertoni L, Cavani F, Resca E, Casolari B, Carnevale G, Zavatti M, Montanari C, Benelli A, and Zanoli P
- Subjects
- Animals, Benzoates chemistry, Body Weight drug effects, Bone and Bones cytology, Bridged Bicyclo Compounds chemistry, Bridged Bicyclo Compounds pharmacology, Cycloheptanes chemistry, Estradiol pharmacology, Female, Organ Size drug effects, Osteoporosis blood, Rats, Rats, Sprague-Dawley, Sesquiterpenes chemistry, Benzoates pharmacology, Bone and Bones drug effects, Bone and Bones metabolism, Cycloheptanes pharmacology, Osteoporosis prevention & control, Ovariectomy, Sesquiterpenes pharmacology
- Abstract
Phytoestrogens play a role in maintaining bone mass in the post-menopausal period for their putative function as osteoprotective agents. The aim of the present study was to investigate the influence of Ferutinin, a phytoestrogen found in the plants of Ferula genus, on bone loss in ovariectomized rats. Such an animal model can simulate the various clinical syndromes deriving from osteoporosis. The effect of the daily oral administration of ferutinin to ovariectomized rats (dosed at 2 mg/kg per day for 30 and 60 days) was compared to that of estradiol benzoate (subcutaneously administered at the dose of 1.5 microg/rat twice a week). After the sacrifice, histomorphometrical analyses were performed on trabecular bone of L4-L5 vertebrae and distal femoral metaphysis, as well as on cortical bone of femoral diaphysis; biochemical parameters (bone mineral components and markers) were also evaluated from the rat serum. The histomorphometrical analyses of trabecular and cortical bone from lumbar vertebrae and femur showed that ferutinin has the same antiosteoporotic effect of estradiol benzoate on bone mass, and in some cases is even stronger. This fact suggests that it could prevent osteoporosis caused by severe estrogen deficiency in ovariectomized rats. The possibility of using ferutinin as an alternative to the commonly employed hormonal replacing therapy in post-menopausal women is discussed.
- Published
- 2009
- Full Text
- View/download PDF
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