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Novel bioprinted 3D model to human fibrosis investigation.

Authors :: Petrachi T
Portone A
Arnaud GF
Ganzerli F
Bergamini V
Resca E
Accorsi L
Ferrari A
Delnevo A
Rovati L
Marra C
Chiavelli C
Dominici M
Veronesi E
Source :: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2023 Sep; Vol. 165, pp. 115146. Date of Electronic Publication: 2023 Jul 17.
Publication Year :: 2023
Abstract: Fibrosis is shared in multiple diseases with progressive tissue stiffening, organ failure and limited therapeutic options. This unmet need is also due to the lack of adequate pre-clinical models to mimic fibrosis and to be challenged novel by anti-fibrotic therapeutic venues. Here using bioprinting, we designed a novel 3D model where normal human healthy fibroblasts have been encapsulated in type I collagen. After stimulation by Transforming Growth factor beta (TGFβ), embedded cells differentiated into myofibroblasts and enhanced the contractile activity, as confirmed by the high level of α - smooth muscle actin (αSMA) and F-actin expression. As functional assays, SEM analysis revealed that after TGFβ stimulus the 3D microarchitecture of the scaffold was dramatically remolded with an increased fibronectin deposition with an abnormal collagen fibrillar pattern. Picrius Sirius Red staining additionally revealed that TGFβ stimulation enhanced of two logarithm the collagen fibrils neoformation in comparison with control. These data indicate that by bioprinting technology, it is possible to generate a reproducible and functional 3D platform to mimic fibrosis as key tool for drug discovery and impacting on animal experimentation and reducing costs and time in addressing fibrosis.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Tiziana Petrachi, Alberto Portone, Gaelle Francoise Arnaud, Francesco Ganzerli, Valentina Bergamini, Elisa Resca, Luca Accorsi, Alberto Ferrari, Annalisa Delnevo, Luigi Rovati, Caterina Marra, Chiara Chiavelli, Massimo Dominici and Elena Veronesi DECLARE NO competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)