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Human amniotic fluid stem cells seeded in fibroin scaffold produce in vivo mineralized matrix.
- Source :
-
Tissue engineering. Part A [Tissue Eng Part A] 2011 Nov; Vol. 17 (21-22), pp. 2833-43. Date of Electronic Publication: 2011 Aug 24. - Publication Year :
- 2011
-
Abstract
- This study investigated the potential of amniotic fluid stem cells (AFSCs) to synthesize mineralized extracellular matrix (ECM) within different porous scaffolds of collagen, poly-D,L-lactic acid (PDLLA), and silk fibroin. The AFSCs were initially differentiated by using an osteogenic medium in two-dimensional culture, and expression of specific bone proteins and the physiologic mineral production by the AFSCs were analyzed. In particular, during differentiation process, AFSCs expressed proteins like Runt-related transcription factor 2 (Runx2), Osterix, Osteopontin, and Osteocalcin with a sequential expression, analogous to those occurring during osteoblast differentiation, and produced extracellular calcium stores. AFSCs were then cultured on three-dimensional (3D) scaffolds and evaluated for their ability to differentiate into osteoblastic cells in vivo. Stem cells were cultured in vitro for 1 week in collagen, fibroin, and PDLLA scaffolds. The effect of predifferentiation of the stem cells in scaffolds on the subsequent bone formation in vivo was determined in a rat subcutaneous model. With the addition of a third dimension, osteogenic differentiation and mineralized ECM production by AFSCs were significantly higher. This study demonstrated the strong potential of AFSCs to produce 3D mineralized bioengineered constructs in vivo and suggests that fibroin may be an effective scaffold material for functional repair of critical size bone defects.
- Subjects :
- Animals
Blotting, Western
Bombyx
Cell Differentiation physiology
Cells, Cultured
Collagen chemistry
Core Binding Factor Alpha 1 Subunit metabolism
Fluorescent Antibody Technique
Humans
Microscopy, Atomic Force
Microscopy, Confocal
Osteocalcin metabolism
Osteogenesis physiology
Osteopontin metabolism
Sp7 Transcription Factor
Transcription Factors metabolism
Amniotic Fluid cytology
Fibroins chemistry
Silk chemistry
Stem Cells cytology
Tissue Scaffolds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1937-335X
- Volume :
- 17
- Issue :
- 21-22
- Database :
- MEDLINE
- Journal :
- Tissue engineering. Part A
- Publication Type :
- Academic Journal
- Accession number :
- 21864161
- Full Text :
- https://doi.org/10.1089/ten.tea.2011.0062