1. The anticancer effect of Salvia pisidica essential oil through promotion intrinsic and extrinsic apoptosis pathways in human cancer cell lines
- Author
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Semiz, G., Mutlu, D., Günal, B., Semiz, A., and Arslan, S.
- Subjects
myrtenol ,Cytotoxic ,carvone ,cineole ,terpenoid derivative ,delta cadinene ,MCF-7 cell line ,Essential oil ,alpha cubebene ,hepatocellular carcinoma cell line ,camphor ,caryaphyllene oxide ,plant medicinal product ,gamma gurjunene ,Salvia ,drug determination ,viridiflorol ,prostate carcinoma cell line ,mycene ,MTT assay ,camphene ,alpha gurjunene ,alpha gualene ,apoptosis ,longifolene ,terpineol ,gamma elemene ,verbenone ,mass fragmentography ,piperitone ,unclassified drug ,real time polymerase chain reaction ,HEK293 cell line ,phellendral ,antitumorigenic activity ,jasmone ,cytotoxicity ,Apoptotic ,beta humulene ,gamma cadinene ,GC-MS ,eugenol ,cancer cell line ,pinene ,colon carcinoma cell line ,in vitro study ,Salvia pisidica ,carvacrol ,embryo ,antineoplastic activity ,absinthol ,lipocortin 5 ,Article ,image cytometry ,Caco-2 cell line ,breast cancer cell line ,chemical composition ,4 cymene 7 ol ,controlled study ,human ,pinocarvone ,beta pinene ,cadalene ,human cell ,borneol ,intrinsic activity ,carveol ,copaene ,ocimene ,cuminaldehyde ,LNCaP cell line ,terpinene ,Complementary and alternative medicine ,aromadendrene ,limonene - Abstract
Introduction: Species of the Salvia genus are aromatic herbs and traditionally used for many medicinal purposes in Anatolia. The genus has produced an extraordinarily large number of beneficial secondary metabolites belonging to numerous chemical groups. Salvia pisidica is an endemic plant of southwest Anatolia, the dried parts of which are used as herbal tea and local materia medica. Methods: The composition of endemic S. pisidica essential oil was determined through GC-MS studies. In vitro cytotoxicity activity and apoptosis analysis were investigated by MTT assay, q-PCR and Annexin V staining experiments. Results: Our results showed that 1,8 cineole (19.15 %) camphor (18.12 %) and γ-gurjunene (8.58 %) were the major constituents of the essential oil. It showed cytotoxic effects on the human breast cancer MCF-7, colon carcinoma Caco-2, hepatocyte carcinoma HepG2, and prostate carcinoma LnCap. The results of q-PCR and image-cytometer analysis showed that the essential oil caused induction of apoptosis by intrinsic and extrinsic pathways in all cancer cell lines. Conclusion: S. pisidica essential oil demonstrates considerable cytotoxic activity due to an apoptosis-related mechanism, suggesting that their bioactive components could be used as natural anticancer substances. © 2023 Elsevier GmbH
- Published
- 2023