Back to Search
Start Over
Radio-protective effect of cinnamic acid, a phenolic phytochemical, on genomic instability induced by X-rays in human blood lymphocytes in vitro
- Source :
- Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 770:72-79
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- The present study was designed to determine the protective activity of cinnamic acid against induction by X-rays of genomic instability in normal human blood lymphocytes. This radio-protective activity was assessed by use of the cytokinesis-block micronucleus test and the alkaline comet assay, with human blood lymphocytes isolated from two healthy donors. A Siemens Mevatron MD2 (Siemens AG, USA, 1994) linear accelerator was used for the irradiation with 1 or 2 Gy. Treatment of the lymphocytes with cinnamic acid prior to irradiation reduced the number of micronuclei when compared with that in control samples. Treatment with cinnamic acid without irradiation did not increase the number of micronuclei and did not show a cytostatic effect in the lymphocytes. The results of the alkaline comet assay revealed that cinnamic acid reduces the DNA damage induced by X-rays, showing a significant radio-protective effect. Cinnamic acid decreased the frequency of irradiation-induced micronuclei by 16-55% and reduced DNA breakage by 17-50%, as determined by the alkaline comet assay. Cinnamic acid may thus act as a radio-protective compound, and future studies may focus on elucidating the mechanism by which cinnamic acid offers radioprotection.
- Subjects :
- Dose-response relationship, radiation
Male
Genome instability
Radioprotection
Micronucleus tests
Health, Toxicology and Mutagenesis
Phytochemicals
Plant medicinal product
Assay
Nuclear division
Micronuclei
Toxicology
Antioxidants
chemistry.chemical_compound
Gamma-radiation
Lymphocytes
High Performance Liquid Chromatography
Palmatine
Berberine
Priority journal
DNA strand breakage
Alkaline comet assay
Biotechnology & applied microbiology
Human blood
Radiation dose
Genetics & heredity
Phenol derivative
Lipid-peroxidation
Normal human
X ray
Phytochemical
Biochemistry
Induced DNA-damage
Micronucleus test
Dichlorofluorescein
Female
Lymphocyte
Cinnamic acid derivative
Derivatives
Human
Genomic instability
Adult
DNA damage
Generation
Alkaline Comet Assay
Article
Cinnamic acid
Linear accelerator
X-ray radiation
Phenols
X-rays
Genetics
Humans
Comet assay
Cytokinesis
Chromosomal damage
Flavonoids
Radiation-protective agents
Radiation protection
Cytostasis
Drug effects
Radiation response
In vitro study
In vitro
Human cell
Micronucleus
chemistry
Cinnamates
Radioprotective agent
Toxicity testing
Acetoacetic acid
Irradiation
Reactive oxygen metabolite
Controlled study
Subjects
Details
- ISSN :
- 13835718
- Volume :
- 770
- Database :
- OpenAIRE
- Journal :
- Mutation Research/Genetic Toxicology and Environmental Mutagenesis
- Accession number :
- edsair.doi.dedup.....74f960834ab94ddd24a08807160fc3d1