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3. Intrinsic Superchirality in Planar Plasmonic Metasurfaces

Author

Palermo, Giovanna, Rippa, Massimo, Aceti, Dante M., Guglielmelli, Alexa, Valente, Liliana, Sagnelli, Domenico, D’Avino, Amalia, Guilcapi, Bryan, Maccaferri, Nicolò, Petti, Lucia, and Strangi, Giuseppe

Abstract

Plasmonic metasurfaces with spatial symmetry breaking are crucial materials with significant applications in fields such as polarization-controlled photonic devices and nanophotonic platforms for chiral sensing. In this paper, we introduce planar plasmonic metasurfaces, less than one-tenth of a wavelength thick, featuring nanocavities formed by three equilateral triangles. This configuration creates uniform, thin metasurfaces. Through a combination of experimental measurements and numerical modeling, we demonstrate the inherent superchirality of these plasmonic metasurfaces. We address the challenge of achieving a strong enhancement of optical chirality in the visible spectrum, reaching levels comparable to those of 3D chiral metasurfaces.

Published
2024
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4. Micro‐ and Nano‐Structured Bacteria Growth Media for Planar Bio‐Photonics

Author

Caligiuri, Vincenzo, primary, Leone, Francesca, additional, Favale, Olga, additional, De Santo, Maria, additional, Bruno, Mauro Daniel Luigi, additional, Mileti, Olga, additional, Pane, Alfredo, additional, Patra, Aniket, additional, Petti, Lucia, additional, Guzman‐Puyol, Susana, additional, Heredia‐Guerrero, José Alejandro, additional, Krahne, Roman, additional, Baldino, Noemi, additional, Bartolino, Roberto, additional, Galluccio, Michele, additional, Annesi, Ferdinanda, additional, and De Luca, Antonio, additional

Published
2023
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6. Micro‐ and Nano‐Structured Bacteria Growth Media for Planar Bio‐Photonics.

Author

Caligiuri, Vincenzo, Leone, Francesca, Favale, Olga, De Santo, Maria, Bruno, Mauro Daniel Luigi, Mileti, Olga, Pane, Alfredo, Patra, Aniket, Petti, Lucia, Guzman‐Puyol, Susana, Heredia‐Guerrero, José Alejandro, Krahne, Roman, Baldino, Noemi, Bartolino, Roberto, Galluccio, Michele, Annesi, Ferdinanda, and De Luca, Antonio

Subjects
PHOTONIC crystals, BIOMATERIALS, BACTERIAL growth, QUASICRYSTALS, BACTERIA, QUANTUM optics
Abstract

Bio‐inspired and biodegradable quantum optics scenarios constitute a pathway toward environmentally friendly front‐end technologies. Such an inspiring perspective necessitates the replacement of classic gain materials with a biological counterpart like photoluminescent bacteria. It is easy to imagine that, in this case, a planar and cell‐viable substitute of classic bulk solid‐states resonators can be highly beneficial. In this paper a micro‐ and nano‐photonic structuration of both a standard and a functionalized version of a typical bacterial growth medium (Luria‐Bertani Agar – LBA) is successfully realized. Three structures belonging to the categories of photonic crystals are replicated, such as quasi‐crystals and meta‐surfaces, demonstrating how the proposed media can be used as templates for high‐end photonic applications. The optical quality of the replicated structures is confirmed by far‐field diffraction measurements. The structured growth media allow for a broad control of the surface wettability by accessing a so‐called Wenzel state, in which the original hydrophilicity of a material is increased due to the photonic structuration. Finally, the suitability of the nano‐structured LBA as a plasmonic platform is evidenced. The proposed micro‐and nano‐structured photonic growth media constitute the first, fundamental step toward quantum optical frameworks from biological media. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Photomobile films based on liquid crystal polymer-carbon black composites

Author

Loffredo, Fausta, primary, De Girolamo Del Mauro, Anna, additional, Villani, Fulvia, additional, Caso, Maria Federica, additional, Fasolino, Tommaso, additional, Miscioscia, Riccardo, additional, Vestri, Ambra, additional, Sagnelli, Domenico, additional, D'Avino, Amalia, additional, Petti, Lucia, additional, and Nenna, Giuseppe, additional

Published
2023
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15. Engineered plasmonic Thue-Morse nanostructures for LSPR detection of the pesticide Thiram

Author

Rippa Massimo, Castagna Riccardo, Pannico Marianna, Musto Pellegrino, Tkachenko Volodymyr, Zhou Jun, and Petti Lucia

Subjects
plasmonic, nanostructures, sensors, lspr, thiram, Physics, QC1-999
Abstract

In this paper, the size- and shape-dependent spectral characteristics of plasmonic nanostructures based on the Thue-Morse (ThMo) sequence are investigated in theory and experiment. We designed, fabricated, and characterized nine different Au nanopillars (NPs) lattices to evaluate their use as nanosensors based on localized surface plasmon resonances (LSPR). The extinction spectra and the bulk refractive index sensitivity (m) are compared to three selected shapes of the NPs (square, circular, and triangular) with different minimum interparticle distance. The maximum m of 275 nm/RIU is obtained for a ThMo pattern with square NPs. Finally, a detection limit of 260 pM (62 pg/ml) of Thiram pesticide has been achieved using an LSPR nanosensor based on an optimized ThMo pattern with triangular NPs employing a phase-sensitive setup to increase the figure-of-merit (FOM) of the sensor.

Published
2017
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17. Large oncosomes overexpressing integrin alpha-V promote prostate cancer adhesion and invasion via AKT activation

Author

Ciardiello, Chiara, Leone, Alessandra, Lanuti, Paola, Roca, Maria S., Moccia, Tania, Minciacchi, Valentina R., Minopoli, Michele, Gigantino, Vincenzo, De Cecio, Rossella, Rippa, Massimo, Petti, Lucia, Capone, Francesca, Vitagliano, Carlo, Milone, Maria R., Pucci, Biagio, Lombardi, Rita, Iannelli, Federica, Di Gennaro, Elena, Bruzzese, Francesca, Marchisio, Marco, Carriero, Maria V., Di Vizio, Dolores, and Budillon, Alfredo

Published
2019
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32. Photo-Responsivity Improvement of Photo-Mobile Polymers Actuators Based on a Novel LCs/Azobenzene Copolymer and ZnO Nanoparticles Network

Author

Sagnelli, Domenico, primary, Calabrese, Marcella, additional, Kaczmarczyk, Olga, additional, Rippa, Massimo, additional, Vestri, Ambra, additional, Marchesano, Valentina, additional, Kortsen, Kristoffer, additional, Cuzzucoli Crucitti, Valentina, additional, Villani, Fulvia, additional, Loffredo, Fausta, additional, Borriello, Carmela, additional, Nenna, Giuseppe, additional, Cocca, Mariacristina, additional, Ambrogi, Veronica, additional, Matczyszyn, Katarzyna, additional, Simoni, Francesco, additional, and Petti, Lucia, additional

Published
2021
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41. Plasmonic Photomobile Polymer Films

Author

Castagna, Riccardo, primary, Rippa, Massimo, additional, Simoni, Francesco, additional, Villani, Fulvia, additional, Nenna, Giuseppe, additional, and Petti, Lucia, additional

Published
2020
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43. Additional file 7: of Large oncosomes overexpressing integrin alpha-V promote prostate cancer adhesion and invasion via AKT activation

Author

Ciardiello, Chiara, Leone, Alessandra, Lanuti, Paola, Roca, Maria, Moccia, Tania, Minciacchi, Valentina, Minopoli, Michele, Gigantino, Vincenzo, Cecio, Rossella De, Rippa, Massimo, Petti, Lucia, Capone, Francesca, Vitagliano, Carlo, Milone, Maria, Pucci, Biagio, Lombardi, Rita, Iannelli, Federica, Gennaro, Elena Di, Bruzzese, Francesca, Marchisio, Marco, Carriero, Maria, Vizio, Dolores Di, and Budillon, Alfredo

Abstract

Figure S6. Kinetic of xenograft tumor growth in nude mice and tumor sample analysis. (A) Hematoxylin and eosin and (B) tumor volume curves of xenograft indicated tumors in nude mice. Negative ÎąV-integrin staining on mice (C) normal lymph node (D) lung ((E) xenograft tumor sample (DU145R80 group) incubated only with secondary antibody. (PDF 186 kb)

Published
2019
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44. Additional file 3: of Large oncosomes overexpressing integrin alpha-V promote prostate cancer adhesion and invasion via AKT activation

Author

Ciardiello, Chiara, Leone, Alessandra, Lanuti, Paola, Roca, Maria, Moccia, Tania, Minciacchi, Valentina, Minopoli, Michele, Gigantino, Vincenzo, Cecio, Rossella De, Rippa, Massimo, Petti, Lucia, Capone, Francesca, Vitagliano, Carlo, Milone, Maria, Pucci, Biagio, Lombardi, Rita, Iannelli, Federica, Gennaro, Elena Di, Bruzzese, Francesca, Marchisio, Marco, Carriero, Maria, Vizio, Dolores Di, and Budillon, Alfredo

Subjects
urologic and male genital diseases, neoplasms
Abstract

Figure S2. Surface integrin profile in DU145 compared to DU145R80 cells. Integrins profile on both DU145 and DU145R80 cell surface. Results are representative of a single experiment performed in triplicate. SD are reported. At least three experiments yielded similar results. (PDF 109 kb)

Published
2019
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45. Additional file 6: of Large oncosomes overexpressing integrin alpha-V promote prostate cancer adhesion and invasion via AKT activation

Author

Ciardiello, Chiara, Leone, Alessandra, Lanuti, Paola, Roca, Maria, Moccia, Tania, Minciacchi, Valentina, Minopoli, Michele, Gigantino, Vincenzo, Cecio, Rossella De, Rippa, Massimo, Petti, Lucia, Capone, Francesca, Vitagliano, Carlo, Milone, Maria, Pucci, Biagio, Lombardi, Rita, Iannelli, Federica, Gennaro, Elena Di, Bruzzese, Francesca, Marchisio, Marco, Carriero, Maria, Vizio, Dolores Di, and Budillon, Alfredo

Abstract

Figure S5. LO induce metalloproteinases activity. (A) LO145 or LOR80 treatment effect on matrix metalloproteinase 9 (MMP9) mRNA expression in DU145 recipient cells. Results are expressed as fold changes of LO-treated DU145 compared to PBS treated DU145 (indicated as =1). The values are the means ± S.D. from at least three independent experiments. (B) Gel zymography assay performed on supernatants from DU145 treated with PBS as vehicle, LO145, LOR80 and 0.5% EtOh-treated DU145 as positive control. (C) Levels of MMPs, as indicated in figures, in DU145 supernatants after treatment with: LO145 (20 μg/mL), LO145 + mAb anti αV-integrin 1:500, LOR80 (20 μg/mL), LOR80 + mAb anti αV-integrin 1:500. MMPs levels were determined using a Bio-Plex array reader. Data are reported as % of decreased levels of single MMPs upon LO pre-exposure with the αV-integrin blocking antibody vs the expression evaluated in LO-treated DU145 (indicated as =1). The values are the means ± S.D. from a single experiment performed in triplicates. (D) Fluorescent gelatin matrix exposed to LOR80, compared to LO145. PBS (CTR) was used as negative control. Proteolitic spots are visualized. Proteases inhibitors cocktail (P.I.) to impair gelatine degradation by LOR80. (E) Size distribution of degradation spots, induced by LO and counted by Image J. (F) Zones of proteolytic clearance in the fluorescent gelatin, expressed as percentage of degradation spots. (PDF 159 kb)

Published
2019
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46. Additional file 2: of Large oncosomes overexpressing integrin alpha-V promote prostate cancer adhesion and invasion via AKT activation

Author

Ciardiello, Chiara, Leone, Alessandra, Lanuti, Paola, Roca, Maria, Moccia, Tania, Minciacchi, Valentina, Minopoli, Michele, Gigantino, Vincenzo, Cecio, Rossella De, Rippa, Massimo, Petti, Lucia, Capone, Francesca, Vitagliano, Carlo, Milone, Maria, Pucci, Biagio, Lombardi, Rita, Iannelli, Federica, Gennaro, Elena Di, Bruzzese, Francesca, Marchisio, Marco, Carriero, Maria, Vizio, Dolores Di, and Budillon, Alfredo

Abstract

Figure S1. Serum free culturing conditions do not induce apoptosis in DU145R80. (A) Apoptosis, evaluated by Annexin-V binding on DU145R80 cells, cultured for 24â h in complete media (CTR), serum free media (SFM) or PBS. The values, expressed as fold changes of control, are the means Âą S.D. from at least three independent experiments. (PDF 84 kb)

Published
2019
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47. Additional file 1: of Large oncosomes overexpressing integrin alpha-V promote prostate cancer adhesion and invasion via AKT activation

Author

Ciardiello, Chiara, Leone, Alessandra, Lanuti, Paola, Roca, Maria, Moccia, Tania, Minciacchi, Valentina, Minopoli, Michele, Gigantino, Vincenzo, Cecio, Rossella De, Rippa, Massimo, Petti, Lucia, Capone, Francesca, Vitagliano, Carlo, Milone, Maria, Pucci, Biagio, Lombardi, Rita, Iannelli, Federica, Gennaro, Elena Di, Bruzzese, Francesca, Marchisio, Marco, Carriero, Maria, Vizio, Dolores Di, and Budillon, Alfredo

Abstract

Supplementary Material and Methods. (DOCX 23 kb)

Published
2019
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48. Additional file 5: of Large oncosomes overexpressing integrin alpha-V promote prostate cancer adhesion and invasion via AKT activation

Author

Ciardiello, Chiara, Leone, Alessandra, Lanuti, Paola, Roca, Maria, Moccia, Tania, Minciacchi, Valentina, Minopoli, Michele, Gigantino, Vincenzo, Cecio, Rossella De, Rippa, Massimo, Petti, Lucia, Capone, Francesca, Vitagliano, Carlo, Milone, Maria, Pucci, Biagio, Lombardi, Rita, Iannelli, Federica, Gennaro, Elena Di, Bruzzese, Francesca, Marchisio, Marco, Carriero, Maria, Vizio, Dolores Di, and Budillon, Alfredo

Abstract

Figure S4. Invasion of diverse prostate recipient cells upon LO exposure (A) Invasion was performed on DU145 cells treated, as indicated in figure, with LO145 untreated or pre-exposed to blocking anti- ÎąV-integrin antibody or to CTR Ab (anti-pAKT antibody ineffective on non-permeabilized intact cells). Results, shown as % of invading cells compared to the vehicle (reported as bar at 100%), are representative of a single experiment performed in triplicates. Several experiments yielded similar results. (B) Invasion was performed at 16â h on both epithelial normal prostate cells (EPN) and PCa LNCaP cells, untreated/treated with LO from both DU145 and DU145R80 cells. Results, reported as fold change of treated cells compared to the vehicle, are representative of a single experiment performed in triplicate and several experiments yielded similar results. (PDF 42 kb)

Published
2019
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49. Additional file 4: of Large oncosomes overexpressing integrin alpha-V promote prostate cancer adhesion and invasion via AKT activation

Author

Ciardiello, Chiara, Leone, Alessandra, Lanuti, Paola, Roca, Maria, Moccia, Tania, Minciacchi, Valentina, Minopoli, Michele, Gigantino, Vincenzo, Cecio, Rossella De, Rippa, Massimo, Petti, Lucia, Capone, Francesca, Vitagliano, Carlo, Milone, Maria, Pucci, Biagio, Lombardi, Rita, Iannelli, Federica, Gennaro, Elena Di, Bruzzese, Francesca, Marchisio, Marco, Carriero, Maria, Vizio, Dolores Di, and Budillon, Alfredo

Abstract

Figure S3. AKT activation upon LOR80 exposure is essential to LO-induced recipient cell adhesion. (A) Western blot analysis for the indicated proteins (30 μg/line) on DU145 cells treated/untreated with either LO145 or LOR80 for 15 min and 60 min. GAPDH served as loading control. (B) Western blot analysis for the indicated proteins (30 μg/line) on DU145 treated/untreated with 50 ng/mL EGF, following pretreatment with GDC-0068 for 6 h or 16 h. Ponceau served as loading control. (C) Adhesion was measured at 1 h in LNCaP cells, untreated/pre-treated for 16 h with the AKT inhibitor GDC-0068 and then treated with either LO145 or LOR80 or PBS as vehicle/control (not shown in the histogram, considered as 100%). Efficacy of treatment was analyzed as % of adherent cells. (PDF 104 kb)

Published
2019
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50. Additional file 8: of Large oncosomes overexpressing integrin alpha-V promote prostate cancer adhesion and invasion via AKT activation

Author

Ciardiello, Chiara, Leone, Alessandra, Lanuti, Paola, Roca, Maria, Moccia, Tania, Minciacchi, Valentina, Minopoli, Michele, Gigantino, Vincenzo, Cecio, Rossella De, Rippa, Massimo, Petti, Lucia, Capone, Francesca, Vitagliano, Carlo, Milone, Maria, Pucci, Biagio, Lombardi, Rita, Iannelli, Federica, Gennaro, Elena Di, Bruzzese, Francesca, Marchisio, Marco, Carriero, Maria, Vizio, Dolores Di, and Budillon, Alfredo

Abstract

Table S1. Clinicopathological features of PCa patients shown in Fig. 6 (B-C panels). (DOCX 56 kb)

Published
2019
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